Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,254,317 T→C T347A (ACG→GCG)  yeiM ← putative transport system permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,254,3170TC100.0% 638.0 / NA 198T347A (ACG→GCG) yeiMputative transport system permease protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (96/102);  total (96/102)

Only 100 of 206 total aligned reads displayed.

AAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGTGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGAT  >  NC_000913/2254228‑2254406
                                                                                         |                                                                                         
aaaTGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCgcc                                                                                        <  2:719908/93‑1 (MQ=255)
       ccAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGtt                                                                                 <  2:628036/93‑1 (MQ=255)
       ccAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGtt                                                                                 <  2:5188442/93‑1 (MQ=255)
       ccAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGtt                                                                                 <  1:5760252/93‑1 (MQ=255)
        cAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGttt                                                                                >  1:1931472/1‑93 (MQ=255)
        cAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCACCCGttt                                                                                <  1:4156541/93‑1 (MQ=255)
         aaCGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTg                                                                               >  2:420398/1‑93 (MQ=255)
           cGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCa                                                                             <  1:795963/93‑1 (MQ=255)
              caacaCCGATAGAACCAAAGTTAGAAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCCCGCCGCCCGTTTGCAGGt                                                                          >  1:965320/1‑93 (MQ=255)
               aacaCCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTa                                                                         <  1:1074197/93‑1 (MQ=255)
               aacaCCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTa                                                                         <  2:1438142/93‑1 (MQ=255)
                acacCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTAt                                                                        >  2:2766895/1‑93 (MQ=255)
                acacCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTAt                                                                        <  1:5036952/93‑1 (MQ=255)
                  acCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGCTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATgg                                                                      >  2:2024522/1‑93 (MQ=255)
                    cGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATgggg                                                                    >  1:5283796/1‑93 (MQ=255)
                    cGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATgggg                                                                    >  1:3715211/1‑93 (MQ=255)
                     gATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGga                                                                   >  2:931428/1‑93 (MQ=255)
                       tAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCTCGTTTGCAGGTATGGGgaga                                                                 >  2:610600/1‑93 (MQ=255)
                       tAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGgaga                                                                 <  2:547856/93‑1 (MQ=255)
                         gAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGaaa                                                               >  2:3792784/1‑93 (MQ=255)
                         aaaCCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGaaa                                                               <  2:4026464/92‑1 (MQ=255)
                           aCCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACt                                                             <  1:1143826/93‑1 (MQ=255)
                           aCCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACt                                                             >  2:1025481/1‑93 (MQ=255)
                             cAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCa                                                           <  2:4710530/93‑1 (MQ=255)
                              aaaGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAg                                                          <  1:3281792/93‑1 (MQ=255)
                              aaaGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAg                                                          <  2:4256840/93‑1 (MQ=255)
                              aaaGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAg                                                          <  2:3861444/93‑1 (MQ=255)
                              aaaGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAg                                                          >  1:5571098/1‑93 (MQ=255)
                               aaGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAgg                                                         <  2:2154474/93‑1 (MQ=255)
                               aaGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAgg                                                         <  1:1528113/93‑1 (MQ=255)
                                aGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGt                                                        >  1:6282870/1‑93 (MQ=255)
                                 gTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTa                                                       <  2:4080198/93‑1 (MQ=255)
                                 gTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTa                                                       >  1:3659285/1‑93 (MQ=255)
                                  ttAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTaa                                                      <  1:3091298/93‑1 (MQ=255)
                                  ttAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTaa                                                      <  1:3551117/93‑1 (MQ=255)
                                    aGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGc                                                    <  2:4807355/93‑1 (MQ=255)
                                       aaaaCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGcgac                                                 <  1:2344335/93‑1 (MQ=255)
                                       aaaaCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGcgac                                                 >  2:4662566/1‑93 (MQ=255)
                                          aCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGaa                                              >  1:523752/1‑93 (MQ=255)
                                          aCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGaa                                              <  1:5424432/93‑1 (MQ=255)
                                          aCCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGaa                                              >  1:4755165/1‑93 (MQ=255)
                                           ccACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAAt                                             <  1:5979105/93‑1 (MQ=255)
                                            cacaAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAAtt                                            >  2:1723086/1‑93 (MQ=255)
                                            cacaAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAAtt                                            <  1:2512472/93‑1 (MQ=255)
                                            cacaAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAAtt                                            >  2:4946355/1‑93 (MQ=255)
                                             ccaAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTc                                           <  2:2096291/92‑1 (MQ=255)
                                               aaaGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGt                                         >  1:5659548/1‑93 (MQ=255)
                                                aaGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGtt                                        <  1:507378/93‑1 (MQ=255)
                                                 aGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTa                                       <  2:4461557/93‑1 (MQ=255)
                                                  acctaaaGGAGATAATCGCAATGGTTTTCAATTCCAGCCCGCCCCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTaa                                      <  2:3887996/89‑1 (MQ=255)
                                                   cgcAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAAt                                     <  2:2727058/93‑1 (MQ=255)
                                                   cgcAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAAt                                     <  1:2085943/93‑1 (MQ=255)
                                                   cgcAAAGGAGATAATCGCAATGGTTTTCACTTACAGCGCACCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAAt                                     <  2:2925086/93‑1 (MQ=255)
                                                    gcAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATc                                    <  2:1857550/93‑1 (MQ=255)
                                                    gcAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATc                                    >  2:2418973/1‑93 (MQ=255)
                                                      aaaGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGc                                  >  1:6071630/1‑93 (MQ=255)
                                                      aaaGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGc                                  >  1:3468696/1‑93 (MQ=255)
                                                       aaGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGcc                                 >  2:5556903/1‑93 (MQ=255)
                                                       aaGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGcc                                 >  1:3111143/1‑93 (MQ=255)
                                                       aaGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGcc                                 <  2:2636025/93‑1 (MQ=255)
                                                         ggAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAg                               <  2:5838260/93‑1 (MQ=255)
                                                          gagaTAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCTGt                              >  2:1176388/1‑93 (MQ=255)
                                                          gagaTAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGt                              >  1:5826701/1‑93 (MQ=255)
                                                          gagaTAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGt                              <  1:4821952/93‑1 (MQ=255)
                                                          gagaTAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGt                              >  2:3298809/1‑93 (MQ=255)
                                                            gaTAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGttt                            <  1:725700/93‑1 (MQ=255)
                                                             aTAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTc                           <  1:5177875/93‑1 (MQ=255)
                                                              tAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCt                          >  1:2272770/1‑93 (MQ=255)
                                                              tAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCt                          >  1:3240283/1‑93 (MQ=255)
                                                                aTCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGc                        >  1:266693/1‑93 (MQ=255)
                                                                  cGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGccc                      <  2:4359386/93‑1 (MQ=255)
                                                                    cAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCaa                    <  2:3896710/93‑1 (MQ=255)
                                                                    cAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCaa                    <  2:1758265/93‑1 (MQ=255)
                                                                     aaTGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAAt                   >  1:943538/1‑93 (MQ=255)
                                                                     aaTGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAAt                   >  2:4549916/1‑93 (MQ=255)
                                                                      aTGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAAta                  >  2:5266267/1‑92 (MQ=255)
                                                                      aTGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATc                  >  2:3121521/1‑93 (MQ=255)
                                                                      aTGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATc                  >  2:89241/1‑93 (MQ=255)
                                                                      aTGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATc                  >  2:5349909/1‑93 (MQ=255)
                                                                      aTGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATc                  >  2:1183138/1‑93 (MQ=255)
                                                                      aTGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATc                  >  2:5208235/1‑93 (MQ=255)
                                                                       tGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCa                 <  2:5017364/93‑1 (MQ=255)
                                                                       tGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCa                 <  2:5968078/93‑1 (MQ=255)
                                                                       tGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCa                 <  2:1700941/93‑1 (MQ=255)
                                                                        ggTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAg                <  1:4598893/93‑1 (MQ=255)
                                                                         gTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAgg               >  1:4464801/1‑93 (MQ=255)
                                                                             tCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTAc           >  1:4962964/1‑93 (MQ=255)
                                                                              cACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTAcc          <  2:2531862/93‑1 (MQ=255)
                                                                              cACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTAcc          <  2:6222166/93‑1 (MQ=255)
                                                                               aCTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTAccc         >  1:366819/1‑93 (MQ=255)
                                                                                 ttCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGc       >  1:2643760/1‑93 (MQ=255)
                                                                                 ttCCAGCGCGCCGCCCGTTGGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGc       <  2:1674082/93‑1 (MQ=255)
                                                                                  tCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCa      >  2:86524/1‑93 (MQ=255)
                                                                                   ccAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCaa     <  1:2944630/93‑1 (MQ=255)
                                                                                   ccAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCaa     <  2:3566929/93‑1 (MQ=255)
                                                                                     aGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACGCGCAAGa   >  1:1502795/1‑93 (MQ=255)
                                                                                     aGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGa   <  1:6083410/93‑1 (MQ=255)
                                                                                     aGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGa   <  1:3832087/93‑1 (MQ=255)
                                                                                      gcgcgcCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGAt  >  2:5648500/1‑93 (MQ=255)
                                                                                      gcgcgcCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGAt  >  1:170386/1‑93 (MQ=255)
                                                                                         |                                                                                         
AAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGTGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGAT  >  NC_000913/2254228‑2254406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGTGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGATTGG  >  NC_000913/2254224‑2254409
                                                                                             |                                                                                            
CCGAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGC                                                                                              <  SRR4302533.2581548/94‑1 (MQ=60)
 CGAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCG                                                                                             >  SRR4302533.979297/1‑94 (MQ=60)
   AAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCC                                                                                           >  SRR4302533.94103/1‑94 (MQ=60)
    AAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCG                                                                                          <  SRR4302533.4016470/94‑1 (MQ=60)
        GCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCG                                                                                      <  SRR4302533.5496931/94‑1 (MQ=60)
          GCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTT                                                                                    >  SRR4302533.5137389/1‑94 (MQ=60)
           CCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTT                                                                                   <  SRR4302533.5795010/94‑1 (MQ=60)
           CCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTT                                                                                   <  SRR4302533.341293/94‑1 (MQ=60)
           CCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTT                                                                                   >  SRR4302533.1943253/1‑94 (MQ=60)
            CAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTG                                                                                  <  SRR4302533.1525006/94‑1 (MQ=60)
            CAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTG                                                                                  >  SRR4302533.4720583/1‑94 (MQ=60)
            CAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTG                                                                                  >  SRR4302533.3685007/1‑94 (MQ=60)
            CAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCACCCGTTTG                                                                                  <  SRR4302533.4181725/94‑1 (MQ=60)
             AACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGC                                                                                 >  SRR4302533.3290854/1‑94 (MQ=60)
               CGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAG                                                                               <  SRR4302533.3388406/94‑1 (MQ=60)
               CGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAG                                                                               <  SRR4302533.800645/94‑1 (MQ=60)
                GACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGG                                                                              <  SRR4302533.4996258/94‑1 (MQ=60)
                 ACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGT                                                                             <  SRR4302533.545574/94‑1 (MQ=60)
                 ACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGT                                                                             <  SRR4302533.5880107/94‑1 (MQ=60)
                 ACAACACCGATAGAACCAAAGTTAGAAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCCCGCCGCCCGTTTGCAGGT                                                                             >  SRR4302533.970984/1‑94 (MQ=60)
                   AACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTAT                                                                           <  SRR4302533.1080530/94‑1 (MQ=60)
                    ACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATG                                                                          <  SRR4302533.954365/94‑1 (MQ=60)
                    ACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATG                                                                          <  SRR4302533.5067454/94‑1 (MQ=60)
                     CACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGG                                                                         <  SRR4302533.233993/94‑1 (MQ=60)
                       CCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGG                                                                       >  SRR4302533.3738018/1‑94 (MQ=60)
                       CCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGG                                                                       >  SRR4302533.5315450/1‑94 (MQ=60)
                            AGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAA                                                                  >  SRR4302533.2664475/1‑94 (MQ=60)
                            AGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAA                                                                  >  SRR4302533.158908/1‑94 (MQ=60)
                             GAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAAC                                                                 >  SRR4302533.4584663/1‑94 (MQ=60)
                              AACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACT                                                                <  SRR4302533.2132148/94‑1 (MQ=60)
                               ACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTC                                                               <  SRR4302533.1150647/94‑1 (MQ=60)
                                 CAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAG                                                             >  SRR4302533.5604771/1‑94 (MQ=60)
                                  AAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGG                                                            <  SRR4302533.3302056/94‑1 (MQ=60)
                                   AAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGT                                                           <  SRR4302533.1537307/94‑1 (MQ=60)
                                   AAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGT                                                           >  SRR4302533.6320364/1‑94 (MQ=60)
                                    AGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTA                                                          >  SRR4302533.3681782/1‑94 (MQ=60)
                                     GTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAA                                                         >  SRR4302533.3527463/1‑94 (MQ=60)
                                      TTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAG                                                        <  SRR4302533.3110307/94‑1 (MQ=60)
                                      TTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAG                                                        <  SRR4302533.3572993/94‑1 (MQ=60)
                                       TAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGC                                                       <  SRR4302533.5269054/94‑1 (MQ=60)
                                       TAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGC                                                       <  SRR4302533.5072954/94‑1 (MQ=60)
                                        AGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCG                                                      <  SRR4302533.2449310/94‑1 (MQ=60)
                                          CAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGAC                                                    <  SRR4302533.128492/94‑1 (MQ=60)
                                           AAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACG                                                   <  SRR4302533.2358844/94‑1 (MQ=60)
                                             AACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAA                                                 >  SRR4302533.526860/1‑94 (MQ=60)
                                             AACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAA                                                 >  SRR4302533.4784021/1‑94 (MQ=60)
                                              ACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAAT                                                <  SRR4302533.5457100/94‑1 (MQ=60)
                                              ACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAAT                                                >  SRR4302533.4981465/1‑94 (MQ=60)
                                               CCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATT                                               <  SRR4302533.6015319/94‑1 (MQ=60)
                                                CACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTC                                              <  SRR4302533.2527951/94‑1 (MQ=60)
                                                 ACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCG                                             >  SRR4302533.3759507/1‑94 (MQ=60)
                                                  CAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGT                                            >  SRR4302533.5693749/1‑94 (MQ=60)
                                                    AAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTA                                          <  SRR4302533.510390/94‑1 (MQ=60)
                                                    AAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTA                                          >  SRR4302533.5634743/1‑94 (MQ=60)
                                                       CGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATC                                       <  SRR4302533.2098789/94‑1 (MQ=60)
                                                        GCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCG                                      >  SRR4302533.2017551/1‑94 (MQ=60)
                                                         CAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGC                                     >  SRR4302533.4924690/1‑94 (MQ=60)
                                                         CAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGC                                     >  SRR4302533.6108362/1‑94 (MQ=60)
                                                         CAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGC                                     >  SRR4302533.3125951/1‑94 (MQ=60)
                                                         CAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGC                                     >  SRR4302533.3490108/1‑94 (MQ=60)
                                                          AAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCC                                    >  SRR4302533.5722903/1‑94 (MQ=60)
                                                          AAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCC                                    >  SRR4302533.3130268/1‑94 (MQ=60)
                                                            AGGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAG                                  >  SRR4302533.4816281/1‑94 (MQ=60)
                                                             GGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGT                                 >  SRR4302533.6290157/1‑94 (MQ=60)
                                                             GGAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGT                                 >  SRR4302533.5861909/1‑94 (MQ=60)
                                                              GAGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTT                                <  SRR4302533.4851171/94‑1 (MQ=60)
                                                               AGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTT                               >  SRR4302533.5264276/1‑94 (MQ=60)
                                                               AGATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGGATGGGGAGAAACTCAGGGAAGCGACGAATTCGTTAATCGCCAGTTT                               >  SRR4302533.3023208/1‑94 (MQ=60)
                                                                GATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTC                              <  SRR4302533.729988/94‑1 (MQ=60)
                                                                 ATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCT                             <  SRR4302533.5209146/94‑1 (MQ=60)
                                                                 ATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCT                             >  SRR4302533.2286836/1‑94 (MQ=60)
                                                                 ATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCT                             <  SRR4302533.5353656/94‑1 (MQ=60)
                                                                 ATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCT                             >  SRR4302533.3260249/1‑94 (MQ=60)
                                                                 ATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCT                             <  SRR4302533.798629/94‑1 (MQ=60)
                                                                 ATAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTACGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGACAGTTTCT                             >  SRR4302533.5811298/1‑94 (MQ=60)
                                                                  TAATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTG                            <  SRR4302533.3623193/94‑1 (MQ=60)
                                                                   AATCGCAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGC                           >  SRR4302533.268313/1‑94 (MQ=60)
                                                                        CAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAAT                      >  SRR4302533.1915462/1‑94 (MQ=60)
                                                                        CAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAAT                      >  SRR4302533.949086/1‑94 (MQ=60)
                                                                        CAATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAAT                      >  SRR4302533.668604/1‑94 (MQ=60)
                                                                          ATGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCA                    <  SRR4302533.294098/94‑1 (MQ=60)
                                                                           TGGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAG                   <  SRR4302533.3207574/94‑1 (MQ=60)
                                                                            GGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGG                  >  SRR4302533.4491806/1‑94 (MQ=60)
                                                                            GGTTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGG                  <  SRR4302533.4626755/94‑1 (MQ=60)
                                                                              TTTTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCT                <  SRR4302533.611473/94‑1 (MQ=60)
                                                                                TTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTAC              >  SRR4302533.4993053/1‑94 (MQ=60)
                                                                                TTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTAC              >  SRR4302533.4430620/1‑94 (MQ=60)
                                                                                TTCACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTAC              >  SRR4302533.2637530/1‑94 (MQ=60)
                                                                                  CACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCC            >  SRR4302533.1003124/1‑94 (MQ=60)
                                                                                  CACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCC            >  SRR4302533.369038/1‑94 (MQ=60)
                                                                                  CACTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCC            >  SRR4302533.3704617/1‑94 (MQ=60)
                                                                                    CTTCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGC          >  SRR4302533.2660004/1‑94 (MQ=60)
                                                                                      TCCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAA        <  SRR4302533.1104051/94‑1 (MQ=60)
                                                                                       CCAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAG       <  SRR4302533.2962600/94‑1 (MQ=60)
                                                                                        CAGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACGCGCAAGA      >  SRR4302533.1511858/1‑94 (MQ=60)
                                                                                         AGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGAT     >  SRR4302533.171458/1‑94 (MQ=60)
                                                                                         AGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGAT     <  SRR4302533.3855511/94‑1 (MQ=60)
                                                                                         AGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGAT     <  SRR4302533.6120193/94‑1 (MQ=60)
                                                                                         AGCGCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGAT     >  SRR4302533.6034025/1‑94 (MQ=60)
                                                                                            GCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGATTGG  >  SRR4302533.6298469/1‑94 (MQ=60)
                                                                                            GCGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGATTGG  <  SRR4302533.1736693/94‑1 (MQ=60)
                                                                                             |                                                                                            
CCGAAAATGCGCCAACGACAACACCGATAGAACCAAAGTTAGCAAAACCACAAAGCGCAAAGGAGATAATCGCAATGGTTTTCACTTCCAGCGTGCCGCCCGTTTGCAGGTATGGGGAGAAACTCAGGTAAGCGACGAATTCGTTAATCGCCAGTTTCTGCCCAATCAGGCTACCCGCAAGATTGG  >  NC_000913/2254224‑2254409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑