Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,299,266 T→C N503S (AAC→AGC)  napA ← putative nitrate reductase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,299,2660TC98.3% 554.3 / ‑2.2 179N503S (AAC→AGC) napAputative nitrate reductase 3
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (79/97);  total (81/98)
Fisher's exact test for biased strand distribution p-value = 5.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 193 total aligned reads displayed.

CCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTC  >  NC_000913/2299176‑2299355
                                                                                          |                                                                                         
cccTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTg                                                                                         >  2:6310407/1‑93 (MQ=255)
 ccTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTgg                                                                                        >  2:3296217/1‑92 (MQ=255)
  ctctTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTgcg                                                                                       >  1:8419207/1‑93 (MQ=255)
   tctTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTgcgc                                                                                      <  2:1378059/93‑1 (MQ=255)
      ttCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGa                                                                                   <  1:7029310/93‑1 (MQ=255)
        ctctACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGGGGAtg                                                                                 >  2:2126954/1‑92 (MQ=255)
        ctctACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATc                                                                                 <  1:96996/93‑1 (MQ=255)
         tctACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATcg                                                                                >  1:9159919/1‑93 (MQ=255)
         tctACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATcg                                                                                <  1:8710828/93‑1 (MQ=255)
           tACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATcgcg                                                                              >  2:6800938/1‑93 (MQ=255)
           tACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGACACGGATCGGAGGCGAAGATGAAGCTGCGCGGATcgcc                                                                              >  1:384338/1‑92 (MQ=255)
            aCCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCgcgc                                                                             <  2:8034583/93‑1 (MQ=255)
             cccACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGcc                                                                            <  1:6100990/93‑1 (MQ=255)
             cccACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGcc                                                                            <  1:5828938/93‑1 (MQ=255)
              ccACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCa                                                                           >  1:8141726/1‑93 (MQ=255)
               cacaTTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAg                                                                          <  1:6419417/93‑1 (MQ=255)
               cacaTTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAg                                                                          <  2:2691345/93‑1 (MQ=255)
               aacaTTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAg                                                                          >  2:100034/2‑93 (MQ=255)
                  aTTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGccc                                                                       <  1:3936753/93‑1 (MQ=255)
                  aTTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGccc                                                                       <  1:3625884/93‑1 (MQ=255)
                  aTTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGccc                                                                       >  1:4543227/1‑93 (MQ=255)
                   ttGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCg                                                                      <  1:8156397/93‑1 (MQ=255)
                      cGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCa                                                                   <  2:9012007/93‑1 (MQ=255)
                      cGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCa                                                                   <  2:5294059/93‑1 (MQ=255)
                      cGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCa                                                                   >  1:535949/1‑93 (MQ=255)
                       ggTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCAt                                                                  <  2:1786740/93‑1 (MQ=255)
                       ggTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCAt                                                                  <  1:7792736/93‑1 (MQ=255)
                        gTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATa                                                                 <  2:4665565/93‑1 (MQ=255)
                        gTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATa                                                                 >  2:8215627/1‑93 (MQ=255)
                            gCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGAACGCGCCAGCCCGGGATACGct                                                             >  2:2452660/1‑93 (MQ=255)
                             cAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCCGCCCGGCATACGctc                                                            >  2:5228122/1‑93 (MQ=255)
                             cAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGctc                                                            <  2:9806433/93‑1 (MQ=255)
                             cAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGctc                                                            >  2:5778072/1‑93 (MQ=255)
                             cAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGccc                                                            >  2:5841114/1‑91 (MQ=255)
                             cAGGATCAAGTCGGCGGCCAGCGCACGGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGctc                                                            <  1:7388075/93‑1 (MQ=255)
                              aGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGctct                                                           <  2:8397029/93‑1 (MQ=255)
                              aGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGctct                                                           >  2:2554546/1‑93 (MQ=255)
                               ggATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCtt                                                          >  1:4880191/1‑93 (MQ=255)
                                gATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTc                                                         >  1:649402/1‑93 (MQ=255)
                                gATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTc                                                         >  2:7501260/1‑93 (MQ=255)
                                   cAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCAtt                                                      >  1:9096500/1‑93 (MQ=255)
                                   cAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACACTCTTCAtt                                                      <  1:4776131/93‑1 (MQ=255)
                                    aaGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATATGCTCTTCATTg                                                     >  2:8942506/1‑93 (MQ=255)
                                    aaGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTg                                                     <  1:2630080/93‑1 (MQ=255)
                                       tCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATATGCTCTTCATTGATg                                                  >  1:8526209/1‑93 (MQ=255)
                                       tCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATg                                                  >  1:3670856/1‑93 (MQ=255)
                                        cggcggCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGt                                                 <  2:5486780/93‑1 (MQ=255)
                                        cggcggCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGt                                                 <  1:2820686/93‑1 (MQ=255)
                                        cggcggCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGt                                                 <  1:9522270/93‑1 (MQ=255)
                                         ggcggcCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGtt                                                <  1:2903880/93‑1 (MQ=255)
                                         ggcggcCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGtt                                                <  2:4138052/93‑1 (MQ=255)
                                          gcggcCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCCTACGCTCTTCATTGATTTTc                                               >  1:180227/1‑93 (MQ=255)
                                          gcggcCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTc                                               <  2:4940754/93‑1 (MQ=255)
                                          gcggcCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTc                                               <  1:5579967/93‑1 (MQ=255)
                                          gcggcCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTc                                               <  2:985502/93‑1 (MQ=255)
                                           cggtCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcg                                              <  2:6776320/93‑1 (MQ=255)
                                            ggcCAGCGCGCTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcgg                                             <  1:5866148/93‑1 (MQ=255)
                                              ccAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCCGCATACGCTCTTCATTGATGTTcggcc                                           >  1:4974538/1‑93 (MQ=255)
                                                aGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcggcccg                                         >  1:2069674/1‑93 (MQ=255)
                                                   gcACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcggcccggcc                                      <  2:9146743/93‑1 (MQ=255)
                                                    cACTGACTGTCGGATACGGATGGGAGTCGATGGTGAAGTTGCGCGGCTGGCGCCACGCCGGCAGCAGCGTTTCCGTGATGTTcggaccggccc                                     >  1:6431043/1‑92 (MQ=255)
                                                    cACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCt                                     <  2:4562291/93‑1 (MQ=255)
                                                     actgactgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTg                                    >  1:4791548/1‑93 (MQ=255)
                                                       tgactgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCa                                  <  2:652585/93‑1 (MQ=255)
                                                        gactgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAt                                 >  2:1726347/1‑93 (MQ=255)
                                                           tgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgtt                              >  2:850378/1‑93 (MQ=255)
                                                            gTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgttg                             >  1:9448065/1‑93 (MQ=255)
                                                            gTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgttg                             >  1:5505561/1‑93 (MQ=255)
                                                            gTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgttg                             <  1:888993/93‑1 (MQ=255)
                                                            gTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgttg                             <  1:9542553/93‑1 (MQ=255)
                                                             tCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgttgt                            <  2:35207/93‑1 (MQ=255)
                                                             tCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgttgt                            <  2:2897623/93‑1 (MQ=255)
                                                                gATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTgg                         >  2:7355913/1‑93 (MQ=255)
                                                                gATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTgg                         >  1:6327084/1‑93 (MQ=255)
                                                                gATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTgg                         <  1:6919092/93‑1 (MQ=255)
                                                                 aTACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGt                        >  1:1941331/1‑93 (MQ=255)
                                                                  tACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTa                       <  2:3700273/93‑1 (MQ=255)
                                                                     ggATCGGAGCCGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTacac                    <  1:3356127/93‑1 (MQ=255)
                                                                     ggATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTacac                    <  2:1127052/93‑1 (MQ=255)
                                                                      gATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTAcaca                   >  1:4448836/1‑93 (MQ=255)
                                                                           gagaCGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTc              >  1:7517017/1‑93 (MQ=255)
                                                                             gaCGATGATGAGGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCa            >  1:244878/1‑93 (MQ=255)
                                                                               cGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGt          <  1:7304804/93‑1 (MQ=255)
                                                                                gatgatGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTa         <  2:400376/93‑1 (MQ=255)
                                                                                 atgatgAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTaa        >  1:1395678/1‑93 (MQ=255)
                                                                                 atgatgAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTaa        >  2:2318403/1‑93 (MQ=255)
                                                                                 atgatgAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTaa        >  1:1585052/1‑93 (MQ=255)
                                                                                 atgatgAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTaa        >  1:2249940/1‑93 (MQ=255)
                                                                                  tgatgaAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTaaa       <  1:1158248/93‑1 (MQ=255)
                                                                                   gatgaAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAAc      <  1:1099532/93‑1 (MQ=255)
                                                                                    atgaAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACa     <  1:4245173/93‑1 (MQ=255)
                                                                                    atgaAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACa     <  1:4177432/93‑1 (MQ=255)
                                                                                    atgaAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACa     >  2:4461406/1‑93 (MQ=255)
                                                                                     tgaAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACAt    <  2:4396319/93‑1 (MQ=255)
                                                                                      taaGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACAtt   <  2:6954252/92‑1 (MQ=255)
                                                                                      gaAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACAtt   <  2:6708341/93‑1 (MQ=255)
                                                                                      gaAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACAtt   <  2:2477934/93‑1 (MQ=255)
                                                                                       aaGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTc  <  1:1479410/93‑1 (MQ=255)
                                                                                       aaGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTc  >  2:6288841/1‑93 (MQ=255)
                                                                                       aaGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTc  <  2:507897/93‑1 (MQ=255)
                                                                                          |                                                                                         
CCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTC  >  NC_000913/2299176‑2299355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTCAGTT  >  NC_000913/2299175‑2299359
                                                                                           |                                                                                             
GCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTG                                                                                             >  SRR4302540.4564955/1‑94 (MQ=60)
  CCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCG                                                                                           >  SRR4302540.8516936/1‑94 (MQ=60)
  CCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCG                                                                                           <  SRR4302540.3796748/94‑1 (MQ=60)
  CCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCG                                                                                           <  SRR4302540.656702/94‑1 (MQ=60)
   CTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGC                                                                                          <  SRR4302540.8714654/94‑1 (MQ=60)
       TTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGAT                                                                                      <  SRR4302540.7107415/94‑1 (MQ=60)
         CTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCG                                                                                    <  SRR4302540.98023/94‑1 (MQ=60)
         CTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCG                                                                                    >  SRR4302540.9269860/1‑94 (MQ=60)
         CTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCG                                                                                    <  SRR4302540.6411093/94‑1 (MQ=60)
          TCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGC                                                                                   <  SRR4302540.8811948/94‑1 (MQ=60)
           CTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCG                                                                                  <  SRR4302540.9552467/94‑1 (MQ=60)
           CTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGACACGGATCGGAGGCGAAGATGAAGCTGCGCGGATCGCC                                                                                  >  SRR4302540.388308/1‑94 (MQ=60)
            TACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGC                                                                                 >  SRR4302540.9238113/1‑94 (MQ=60)
              CCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCA                                                                               <  SRR4302540.6168919/94‑1 (MQ=60)
              CCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCA                                                                               <  SRR4302540.5893376/94‑1 (MQ=60)
              CCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCA                                                                               >  SRR4302540.8236227/1‑94 (MQ=60)
               CCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAG                                                                              <  SRR4302540.7545231/94‑1 (MQ=60)
                CACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGC                                                                             <  SRR4302540.6490867/94‑1 (MQ=60)
                CACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGC                                                                             <  SRR4302540.6490868/94‑1 (MQ=60)
                  CATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCC                                                                           >  SRR4302540.8605314/1‑94 (MQ=60)
                  CATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCC                                                                           >  SRR4302540.4592390/1‑94 (MQ=60)
                   ATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCG                                                                          <  SRR4302540.3979297/94‑1 (MQ=60)
                   ATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCG                                                                          <  SRR4302540.3665569/94‑1 (MQ=60)
                    TTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGG                                                                         <  SRR4302540.8251093/94‑1 (MQ=60)
                      GCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCA                                                                       >  SRR4302540.541754/1‑94 (MQ=60)
                        GGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATA                                                                     <  SRR4302540.1589233/94‑1 (MQ=60)
                        GGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATA                                                                     <  SRR4302540.7883827/94‑1 (MQ=60)
                          TCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACG                                                                   <  SRR4302540.4871082/94‑1 (MQ=60)
                           CGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGC                                                                  <  SRR4302540.9709183/94‑1 (MQ=60)
                            GGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCT                                                                 >  SRR4302540.774512/1‑94 (MQ=60)
                              CAGGATCAAGTCGGCGGCCAGCGCACGGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCT                                                               <  SRR4302540.7470392/94‑1 (MQ=60)
                               AGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTT                                                              >  SRR4302540.4932587/1‑94 (MQ=60)
                                GGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTC                                                             >  SRR4302540.656312/1‑94 (MQ=60)
                                   TCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATT                                                          >  SRR4302540.9205797/1‑94 (MQ=60)
                                    CAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTG                                                         >  SRR4302540.1971476/1‑94 (MQ=60)
                                    CAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTG                                                         <  SRR4302540.3577698/94‑1 (MQ=60)
                                    CAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACACTCTTCATTG                                                         <  SRR4302540.4827162/94‑1 (MQ=60)
                                     AAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGA                                                        <  SRR4302540.2658546/94‑1 (MQ=60)
                                     AAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGA                                                        >  SRR4302540.1410553/1‑94 (MQ=60)
                                      AGTCGGCGGCCAGCGCACTGACTGTCGGATACGGACCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGAT                                                       <  SRR4302540.4842215/94‑1 (MQ=60)
                                       GTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATATGCTCTTCATTGATG                                                      >  SRR4302540.8625279/1‑94 (MQ=60)
                                       GTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATG                                                      <  SRR4302540.3911142/94‑1 (MQ=60)
                                       GTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATG                                                      >  SRR4302540.3711009/1‑94 (MQ=60)
                                       GTCGGCGGCCAGCGCACTGACTCTCGCATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATG                                                      <  SRR4302540.24772/94‑1 (MQ=60)
                                         cggcgtgcacCGCACTGACGGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTT                                                    <  SRR4302540.2851152/84‑1 (MQ=60)
                                         CGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTT                                                    <  SRR4302540.9635027/94‑1 (MQ=60)
                                         CGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTT                                                    <  SRR4302540.9635026/94‑1 (MQ=60)
                                         CGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTT                                                    <  SRR4302540.2851154/94‑1 (MQ=60)
                                          GGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCCTACGCTCTTCATTGATTTTC                                                   >  SRR4302540.182019/1‑94 (MQ=60)
                                          GGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTC                                                   <  SRR4302540.2935578/94‑1 (MQ=60)
                                           GCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCG                                                  <  SRR4302540.5641387/94‑1 (MQ=60)
                                             GGCCAGCGCGCTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGC                                                <  SRR4302540.5931002/94‑1 (MQ=60)
                                              GCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCCGCATACGCTCTTCATTGATGTTCGGCC                                               >  SRR4302540.5028375/1‑94 (MQ=60)
                                              GCCAGCGCACTGACTGTCGGACACGGATCTGAGACGACGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTtggtc                                               >  SRR4302540.1057731/1‑89 (MQ=60)
                                               CCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCC                                              >  SRR4302540.5506781/1‑94 (MQ=60)
                                                CAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCG                                             >  SRR4302540.2091934/1‑94 (MQ=60)
                                                  GCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGGTGTTCGGCCCGGC                                           >  SRR4302540.4637679/1‑94 (MQ=60)
                                                   CGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCC                                          >  SRR4302540.4202392/1‑94 (MQ=60)
                                                    GCACTGACTGTCGGATACGGATGGGAGTCGATGGTGAAGTTGCGCGGctggcgccacgccggcagcagcgtttccgtgatgttcggaccggccc                                         >  SRR4302540.6502623/1‑47 (MQ=60)
                                                    GCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCT                                         <  SRR4302540.1777936/94‑1 (MQ=60)
                                                    GCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCT                                         >  SRR4302540.7466661/1‑94 (MQ=60)
                                                     CACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTG                                        >  SRR4302540.4842797/1‑94 (MQ=60)
                                                      ACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTTATGTTCGGCCCGGCCGGC                                       >  SRR4302540.2149108/1‑94 (MQ=60)
                                                      ACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGC                                       >  SRR4302540.1438875/1‑94 (MQ=60)
                                                            TGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTG                                 >  SRR4302540.562522/1‑94 (MQ=60)
                                                            TGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTG                                 >  SRR4302540.5566193/1‑94 (MQ=60)
                                                            TGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTG                                 >  SRR4302540.9560227/1‑94 (MQ=60)
                                                            TGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTG                                 >  SRR4302540.9560225/1‑94 (MQ=60)
                                                            TGTCGGATACGGATCGGAGACGATGAGGAAGCGGCGCGGAGCGCGCCAGCCCGGCATACGCTCTTCATTGAGGTTCGGCCCGGCCTGCATGTTG                                 <  SRR4302540.6290420/94‑1 (MQ=60)
                                                             GTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGT                                <  SRR4302540.898438/94‑1 (MQ=60)
                                                             GTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGT                                <  SRR4302540.9655467/94‑1 (MQ=60)
                                                               TGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTG                              <  SRR4302540.6115311/94‑1 (MQ=60)
                                                               CGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTG                              >  SRR4302540.6509482/1‑94 (MQ=60)
                                                                GGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGG                             >  SRR4302540.6397545/1‑94 (MQ=60)
                                                                GGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGG                             >  SRR4302540.4936345/1‑94 (MQ=60)
                                                                 GATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGT                            >  SRR4302540.1962473/1‑94 (MQ=60)
                                                                 GATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGT                            <  SRR4302540.6996081/94‑1 (MQ=60)
                                                                   TACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTAC                          <  SRR4302540.423021/94‑1 (MQ=60)
                                                                      GGATCGGAGCCGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACA                       <  SRR4302540.3392901/94‑1 (MQ=60)
                                                                      GGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACA                       >  SRR4302540.4497052/1‑94 (MQ=60)
                                                                           GGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTC                  >  SRR4302540.7601064/1‑94 (MQ=60)
                                                                             AGACGATGATGAGGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCA                >  SRR4302540.247243/1‑94 (MQ=60)
                                                                             AGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCA                >  SRR4302540.5280913/1‑94 (MQ=60)
                                                                              GACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAG               <  SRR4302540.2924438/94‑1 (MQ=60)
                                                                                CGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTA             <  SRR4302540.7386349/94‑1 (MQ=60)
                                                                                CGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTA             >  SRR4302540.7290082/1‑94 (MQ=60)
                                                                                CGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTA             <  SRR4302540.7040814/94‑1 (MQ=60)
                                                                                 GATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAA            >  SRR4302540.1410644/1‑94 (MQ=60)
                                                                                 GATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAA            >  SRR4302540.2274098/1‑94 (MQ=60)
                                                                                 GATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAA            >  SRR4302540.2274096/1‑94 (MQ=60)
                                                                                 GATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAA            >  SRR4302540.1602365/1‑94 (MQ=60)
                                                                                   TGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAAC          <  SRR4302540.1170292/94‑1 (MQ=60)
                                                                                   TGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAAC          <  SRR4302540.9117244/94‑1 (MQ=60)
                                                                                   TGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAAC          >  SRR4302540.10052588/1‑94 (MQ=60)
                                                                                   TGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAAC          >  SRR4302540.8431219/1‑94 (MQ=60)
                                                                                    GATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACA         <  SRR4302540.1110854/94‑1 (MQ=60)
                                                                                     ATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACAT        <  SRR4302540.4222782/94‑1 (MQ=60)
                                                                                     ATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACAT        <  SRR4302540.4291357/94‑1 (MQ=60)
                                                                                     ATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACAT        <  SRR4302540.4404030/94‑1 (MQ=60)
                                                                                      TGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATT       >  SRR4302540.8947299/1‑94 (MQ=60)
                                                                                      TGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATT       <  SRR4302540.2980925/94‑1 (MQ=60)
                                                                                      TGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAAAATT       >  SRR4302540.5895591/1‑94 (MQ=60)
                                                                                       GAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTA      >  SRR4302540.1882478/1‑94 (MQ=60)
                                                                                        AAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTCA     <  SRR4302540.1495612/94‑1 (MQ=60)
                                                                                        AAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTCA     >  SRR4302540.10009308/1‑94 (MQ=60)
                                                                                           CTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTCAGTT  >  SRR4302540.1113842/1‑94 (MQ=60)
                                                                                           CTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTCAGTT  <  SRR4302540.4756360/94‑1 (MQ=60)
                                                                                           |                                                                                             
GCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTCAGTT  >  NC_000913/2299175‑2299359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑