Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,299,266 T→C N503S (AAC→AGC)  napA ← putative nitrate reductase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,299,2660TC100.0% 608.1 / NA 189N503S (AAC→AGC) napAputative nitrate reductase 3
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (88/101);  total (88/101)

Only 100 of 204 total aligned reads displayed.

CCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTC  >  NC_000913/2299176‑2299355
                                                                                          |                                                                                         
cccTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGGCGATGATGAAGCTg                                                                                         >  2:318229/1‑93 (MQ=255)
cccTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGGCGATGATGAAGCTg                                                                                         >  2:5846284/1‑93 (MQ=255)
cccTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTg                                                                                         >  1:5605792/1‑93 (MQ=255)
 ccTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATTAAGCTgc                                                                                        >  1:4345870/1‑93 (MQ=255)
 ccTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTgc                                                                                        <  2:3777269/93‑1 (MQ=255)
 ccTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGAAGATGATGAAGCTgg                                                                                        >  2:4502784/1‑92 (MQ=255)
  ctctTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTgcg                                                                                       <  2:3375652/93‑1 (MQ=255)
  ctctTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTgcg                                                                                       <  2:2523402/93‑1 (MQ=255)
  ctctTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAACTgcg                                                                                       >  2:5969263/1‑93 (MQ=255)
  ctctTTCTCTACCCACATGGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTgcg                                                                                       <  2:122508/93‑1 (MQ=255)
   tctTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGgcgcgc                                                                                      >  2:4877901/1‑93 (MQ=255)
   tctTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTgcgc                                                                                      <  1:928062/93‑1 (MQ=255)
   tctTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTgcgc                                                                                      >  2:6030430/1‑93 (MQ=255)
       tctcTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGAt                                                                                  <  2:5338561/93‑1 (MQ=255)
       tctcTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGAt                                                                                  <  2:2922470/93‑1 (MQ=255)
        ctctACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATc                                                                                 <  2:4591094/93‑1 (MQ=255)
         tctACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATcg                                                                                >  2:6231094/1‑93 (MQ=255)
         tctACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATcg                                                                                <  1:143999/93‑1 (MQ=255)
          ctACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATcgc                                                                               <  2:5017392/93‑1 (MQ=255)
           tACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATcgcg                                                                              <  1:1205995/93‑1 (MQ=255)
            aCCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCgcgc                                                                             >  2:2443287/1‑93 (MQ=255)
            aCCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCgcgc                                                                             >  2:2054330/1‑93 (MQ=255)
            aCCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCgcgc                                                                             <  2:1460258/93‑1 (MQ=255)
             cccACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGcc                                                                            <  1:1549430/93‑1 (MQ=255)
               cacaTTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAg                                                                          >  1:5224811/1‑93 (MQ=255)
                 caTTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGcc                                                                        >  1:1144987/1‑93 (MQ=255)
                  aTTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGccc                                                                       >  1:5920189/1‑93 (MQ=255)
                    tGCGGTCAGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCgg                                                                     <  1:1650657/93‑1 (MQ=255)
                     gCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGc                                                                    <  2:1614617/93‑1 (MQ=255)
                     gCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGc                                                                    <  2:5096463/93‑1 (MQ=255)
                       ggTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCAt                                                                  >  2:2036405/1‑93 (MQ=255)
                        gTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATa                                                                 <  2:5445326/93‑1 (MQ=255)
                         tCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATAc                                                                <  2:2769797/93‑1 (MQ=255)
                         tCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATAc                                                                <  1:1085524/93‑1 (MQ=255)
                         tCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATAc                                                                <  2:5929669/93‑1 (MQ=255)
                          cGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACg                                                               <  2:4616382/93‑1 (MQ=255)
                           ggCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCACACGc                                                              >  1:55451/1‑93 (MQ=255)
                            gCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGct                                                             >  1:2873676/1‑93 (MQ=255)
                              aGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGctct                                                           >  1:4416495/1‑93 (MQ=255)
                               ggATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGCGACGATGATGAAGCTGCGCGGAGCGCGCCAGCCCGGCATACGCTCtt                                                          >  2:3640663/1‑93 (MQ=255)
                               ggATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCtt                                                          >  1:310868/1‑93 (MQ=255)
                                gATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTc                                                         <  1:6283630/93‑1 (MQ=255)
                                     aGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGa                                                    >  2:4991861/1‑93 (MQ=255)
                                     aGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGa                                                    <  1:4551962/93‑1 (MQ=255)
                                      gTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGAt                                                   <  1:2334198/93‑1 (MQ=255)
                                       tCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATg                                                  <  1:2860112/93‑1 (MQ=255)
                                          gcggcCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTc                                               <  1:2716599/93‑1 (MQ=255)
                                          gcggcCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTc                                               >  1:2411169/1‑93 (MQ=255)
                                           cggcCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcg                                              <  1:261805/93‑1 (MQ=255)
                                               cAGCCCCCTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcggccc                                          <  1:3899420/93‑1 (MQ=255)
                                                aGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcggcccg                                         >  2:5367613/1‑93 (MQ=255)
                                                 gcgcACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcggcccgg                                        <  2:2188678/93‑1 (MQ=255)
                                                  cgcACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcggcccggc                                       >  1:3308737/1‑93 (MQ=255)
                                                  cgcACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcggcccggc                                       <  1:1585908/93‑1 (MQ=255)
                                                   gcACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTcggcccggcc                                      <  2:5697289/93‑1 (MQ=255)
                                                     actgactgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTg                                    >  1:5463707/1‑93 (MQ=255)
                                                      ctgactgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGc                                   >  2:3272499/1‑93 (MQ=255)
                                                       tgactgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCa                                  >  1:5431606/1‑93 (MQ=255)
                                                        gactgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGGCTGCAt                                 >  2:2687581/1‑93 (MQ=255)
                                                         actgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtg                                >  1:4753371/1‑93 (MQ=255)
                                                          ctgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgt                               >  1:3646748/1‑93 (MQ=255)
                                                           tgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgtt                              <  2:2598099/93‑1 (MQ=255)
                                                           tgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgtt                              >  1:3554980/1‑93 (MQ=255)
                                                           tgTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgtt                              >  1:6005416/1‑93 (MQ=255)
                                                           tgTCGGATACGGATCGGAGACGATAATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgtt                              >  2:1913192/1‑93 (MQ=255)
                                                           tgTCCGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgtt                              <  1:3732553/93‑1 (MQ=255)
                                                            gTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgttg                             >  1:5806597/1‑93 (MQ=255)
                                                            gTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgttg                             >  2:3164956/1‑93 (MQ=255)
                                                            gTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgttg                             <  2:1097723/93‑1 (MQ=255)
                                                            gTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgttg                             >  1:4134444/1‑93 (MQ=255)
                                                             tCGGAGACGTATCGGAGACGATGATGAAGCTGCGCGGATCGCGACAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAtgttgt                            <  1:3024273/93‑1 (MQ=255)
                                                              cGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATgttgtt                           <  2:3146836/93‑1 (MQ=255)
                                                               ggATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGttgttg                          <  2:1277375/93‑1 (MQ=255)
                                                               ggATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGttgttg                          <  2:2670337/93‑1 (MQ=255)
                                                                gATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTgg                         <  1:1269593/93‑1 (MQ=255)
                                                                gATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTgg                         >  1:3145558/1‑93 (MQ=255)
                                                                 aTACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGt                        <  2:5652544/93‑1 (MQ=255)
                                                                   aCGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTac                      >  2:274701/1‑93 (MQ=255)
                                                                   aCGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTac                      <  2:4499200/93‑1 (MQ=255)
                                                                    cGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTaca                     >  1:2265662/1‑93 (MQ=255)
                                                                     ggATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTacac                    >  2:3499070/1‑93 (MQ=255)
                                                                     ggATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTacac                    >  1:674996/1‑93 (MQ=255)
                                                                       aTCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACAt                  <  1:2151231/93‑1 (MQ=255)
                                                                       aTCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACAt                  <  1:323233/93‑1 (MQ=255)
                                                                        tCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGCTGTTGGTACACATg                 >  1:4062647/1‑93 (MQ=255)
                                                                          ggAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGt               <  2:4299465/93‑1 (MQ=255)
                                                                           gagaCGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTc              >  2:4022939/1‑93 (MQ=255)
                                                                           gagaCGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTc              <  1:2799071/93‑1 (MQ=255)
                                                                            agaCGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTcc             >  1:1356910/1‑93 (MQ=255)
                                                                             gaCGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCa            <  2:3207563/93‑1 (MQ=255)
                                                                                gatgatGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTa         >  1:4948023/1‑93 (MQ=255)
                                                                                 atgatgAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTaa        >  2:1339402/1‑93 (MQ=255)
                                                                                  tgatgaAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTaaa       >  2:43671/1‑93 (MQ=255)
                                                                                  tgatgaAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTaaa       <  2:3789063/93‑1 (MQ=255)
                                                                                  tgatgaAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTaaa       <  1:4597238/93‑1 (MQ=255)
                                                                                   gatgaAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAAc      >  1:48216/1‑93 (MQ=255)
                                                                                     tgaAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACAt    <  2:2893871/93‑1 (MQ=255)
                                                                                     tgaAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACAt    >  2:629091/1‑93 (MQ=255)
                                                                                      gaAGCTGCGCGGATAGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACAtt   <  1:5259259/93‑1 (MQ=255)
                                                                                       aaGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTc  <  1:6109837/93‑1 (MQ=255)
                                                                                          |                                                                                         
CCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTC  >  NC_000913/2299176‑2299355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTCAGTT  >  NC_000913/2299174‑2299359
                                                                                            |                                                                                             
CGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCT                                                                                              <  SRR4302533.633492/94‑1 (MQ=60)
CGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCT                                                                                              <  SRR4302533.505783/94‑1 (MQ=60)
CGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCT                                                                                              >  SRR4302533.3160248/1‑94 (MQ=60)
 GCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTG                                                                                             >  SRR4302533.5639665/1‑94 (MQ=60)
 GCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTG                                                                                             <  SRR4302533.1457066/94‑1 (MQ=60)
  CCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATTAAGCTGC                                                                                            >  SRR4302533.4372065/1‑94 (MQ=60)
  CCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGC                                                                                            >  SRR4302533.5561934/1‑94 (MQ=60)
   CCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCG                                                                                           <  SRR4302533.5041785/94‑1 (MQ=60)
     TCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCG                                                                                         <  SRR4302533.933512/94‑1 (MQ=60)
          CTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCG                                                                                    >  SRR4302533.3712731/1‑94 (MQ=60)
           TCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGC                                                                                   <  SRR4302533.144922/94‑1 (MQ=60)
            CTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCG                                                                                  >  SRR4302533.4132764/1‑94 (MQ=60)
             TACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGC                                                                                 <  SRR4302533.1213239/94‑1 (MQ=60)
               CCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCA                                                                               <  SRR4302533.1558769/94‑1 (MQ=60)
               CCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCA                                                                               <  SRR4302533.1606756/94‑1 (MQ=60)
                CCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAG                                                                              >  SRR4302533.5256254/1‑94 (MQ=60)
                  ACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCC                                                                            >  SRR4302533.1151822/1‑94 (MQ=60)
                   CATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCC                                                                           >  SRR4302533.5955973/1‑94 (MQ=60)
                   CATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCC                                                                           <  SRR4302533.2438348/94‑1 (MQ=60)
                     TTGCGGCCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGG                                                                         <  SRR4302533.3886283/94‑1 (MQ=60)
                      TGCGGTCAGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGC                                                                        <  SRR4302533.1660656/94‑1 (MQ=60)
                           TCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCcgaccgggataag                                                                   >  SRR4302533.1100808/1‑81 (MQ=60)
                           TCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACG                                                                   <  SRR4302533.1091925/94‑1 (MQ=60)
                            CGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCACACGC                                                                  >  SRR4302533.55823/1‑94 (MQ=60)
                             GGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCT                                                                 >  SRR4302533.3684008/1‑94 (MQ=60)
                             GGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCT                                                                 >  SRR4302533.2891356/1‑94 (MQ=60)
                             GGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCT                                                                 <  SRR4302533.482326/94‑1 (MQ=60)
                              GCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTC                                                                >  SRR4302533.17202/1‑94 (MQ=60)
                               CAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCT                                                               >  SRR4302533.4443197/1‑94 (MQ=60)
                               CAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCT                                                               <  SRR4302533.3600927/94‑1 (MQ=60)
                                AGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTT                                                              >  SRR4302533.312771/1‑94 (MQ=60)
                                  GATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCA                                                            <  SRR4302533.6321125/94‑1 (MQ=60)
                                      AAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGA                                                        <  SRR4302533.5958910/94‑1 (MQ=60)
                                      AAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGA                                                        >  SRR4302533.6327556/1‑94 (MQ=60)
                                       AGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGAT                                                       <  SRR4302533.4653807/94‑1 (MQ=60)
                                       AGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGAT                                                       <  SRR4302533.4579569/94‑1 (MQ=60)
                                       AGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGAT                                                       <  SRR4302533.5770319/94‑1 (MQ=60)
                                        GTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATG                                                      <  SRR4302533.2348652/94‑1 (MQ=60)
                                         TCGGCGGCCAGCGCACTGACTGTCGGATACTGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGT                                                     <  SRR4302533.2877709/94‑1 (MQ=60)
                                         TCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGT                                                     <  SRR4302533.2877708/94‑1 (MQ=60)
                                           GGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTC                                                   >  SRR4302533.2426061/1‑94 (MQ=60)
                                            GCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCG                                                  <  SRR4302533.2733277/94‑1 (MQ=60)
                                             CGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGG                                                 <  SRR4302533.263392/94‑1 (MQ=60)
                                              GGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGC                                                <  SRR4302533.1376022/94‑1 (MQ=60)
                                               GCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCC                                               >  SRR4302533.528225/1‑94 (MQ=60)
                                                 CAGCCCCCTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCG                                             <  SRR4302533.3923214/94‑1 (MQ=60)
                                                  AGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGG                                            <  SRR4302533.2714885/94‑1 (MQ=60)
                                                  AGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGG                                            >  SRR4302533.1338382/1‑94 (MQ=60)
                                                  AGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGG                                            >  SRR4302533.562700/1‑94 (MQ=60)
                                                   GCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGC                                           >  SRR4302533.3329166/1‑94 (MQ=60)
                                                    CGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCC                                          >  SRR4302533.4866004/1‑94 (MQ=60)
                                                    CGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCC                                          <  SRR4302533.1595463/94‑1 (MQ=60)
                                                    CGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCC                                          >  SRR4302533.1593631/1‑94 (MQ=60)
                                                     GCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCT                                         <  SRR4302533.3230061/94‑1 (MQ=60)
                                                     GCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCT                                         <  SRR4302533.2746576/94‑1 (MQ=60)
                                                     GCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCT                                         <  SRR4302533.4614660/94‑1 (MQ=60)
                                                      CACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTG                                        >  SRR4302533.5496630/1‑94 (MQ=60)
                                                       ACTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGC                                       <  SRR4302533.4430042/94‑1 (MQ=60)
                                                        CTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCA                                      >  SRR4302533.220075/1‑94 (MQ=60)
                                                        CTGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCA                                      >  SRR4302533.5464315/1‑94 (MQ=60)
                                                         TGACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCAT                                     <  SRR4302533.4519076/94‑1 (MQ=60)
                                                          GACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATG                                    >  SRR4302533.4782187/1‑94 (MQ=60)
                                                           ACTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGT                                   >  SRR4302533.3669169/1‑94 (MQ=60)
                                                            CTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTT                                  >  SRR4302533.6041804/1‑94 (MQ=60)
                                                            CTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTT                                  <  SRR4302533.5492897/94‑1 (MQ=60)
                                                            CTGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTT                                  >  SRR4302533.3576889/1‑94 (MQ=60)
                                                             TGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTG                                 <  SRR4302533.1270953/94‑1 (MQ=60)
                                                             TGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTG                                 >  SRR4302533.4159539/1‑94 (MQ=60)
                                                             TGTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTG                                 >  SRR4302533.5841660/1‑94 (MQ=60)
                                                             TGTCCGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTG                                 <  SRR4302533.3755452/94‑1 (MQ=60)
                                                              GTCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGGTCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGTCCGGGCTGCATGTTGT                                >  SRR4302533.2384132/1‑94 (MQ=60)
                                                               TCGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTT                               <  SRR4302533.229056/94‑1 (MQ=60)
                                                               TCGGAGACGTATCGGAGACGATGATGAAGCTGCGCGGATCGCGACAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTT                               <  SRR4302533.3042776/94‑1 (MQ=60)
                                                                CGGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTG                              >  SRR4302533.5928902/1‑94 (MQ=60)
                                                                 GGATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGG                             >  SRR4302533.3164897/1‑94 (MQ=60)
                                                                  GATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGT                            <  SRR4302533.1277190/94‑1 (MQ=60)
                                                                   ATACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTA                           >  SRR4302533.345981/1‑94 (MQ=60)
                                                                     ACGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACA                         >  SRR4302533.2279680/1‑94 (MQ=60)
                                                                      CGGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACAC                        >  SRR4302533.678984/1‑94 (MQ=60)
                                                                       GGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACA                       <  SRR4302533.2835230/94‑1 (MQ=60)
                                                                       GGATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACA                       <  SRR4302533.3131519/94‑1 (MQ=60)
                                                                        GATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACAT                      <  SRR4302533.6158649/94‑1 (MQ=60)
                                                                        GATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACAT                      >  SRR4302533.4334574/1‑94 (MQ=60)
                                                                        GATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACAT                      >  SRR4302533.3957038/1‑94 (MQ=60)
                                                                         ATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATG                     <  SRR4302533.2164479/94‑1 (MQ=60)
                                                                         ATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATG                     <  SRR4302533.325204/94‑1 (MQ=60)
                                                                         ATCGGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGCTGTTGGTACACATG                     >  SRR4302533.4087377/1‑94 (MQ=60)
                                                                            GGAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTC                  >  SRR4302533.294143/1‑94 (MQ=60)
                                                                             GAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCC                 <  SRR4302533.2816287/94‑1 (MQ=60)
                                                                             GAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCC                 >  SRR4302533.1365064/1‑94 (MQ=60)
                                                                             GAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCC                 <  SRR4302533.3872475/94‑1 (MQ=60)
                                                                             GAGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCC                 >  SRR4302533.1213192/1‑94 (MQ=60)
                                                                              AGACGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCA                >  SRR4302533.3310166/1‑94 (MQ=60)
                                                                                 CGATGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTA             >  SRR4302533.4977984/1‑94 (MQ=60)
                                                                                    TGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAAC          <  SRR4302533.750527/94‑1 (MQ=60)
                                                                                    TGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAAC          >  SRR4302533.1474023/1‑94 (MQ=60)
                                                                                    TGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAAC          >  SRR4302533.48550/1‑94 (MQ=60)
                                                                                    TGATGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAAC          <  SRR4302533.4625086/94‑1 (MQ=60)
                                                                                       TGAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATT       <  SRR4302533.306589/94‑1 (MQ=60)
                                                                                        GAAGCTGCGCGGATCGCGCCAGCCCGTCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTC      <  SRR4302533.6065595/94‑1 (MQ=60)
                                                                                        GAAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTC      <  SRR4302533.5380287/94‑1 (MQ=60)
                                                                                        GAAGCTGCGCGGATAGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTC      <  SRR4302533.5290837/94‑1 (MQ=60)
                                                                                         AAGCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTCA     <  SRR4302533.6146720/94‑1 (MQ=60)
                                                                                           GCTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTCAGT   <  SRR4302533.4697410/94‑1 (MQ=60)
                                                                                            CTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTCAGTT  >  SRR4302533.4855869/1‑94 (MQ=60)
                                                                                            |                                                                                             
CGCCCTCTTTCTCTACCCACATTGCGGTCGGCAGGATCAAGTCGGCGGCCAGCGCACTGACTGTCGGATACGGATCGGAGACGATGATGAAGTTGCGCGGATCGCGCCAGCCCGGCATACGCTCTTCATTGATGTTCGGCCCGGCCTGCATGTTGTTGGTACACATGGTCCAGTAAACATTCAGTT  >  NC_000913/2299174‑2299359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑