Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,324,469 (G)6→7 coding (341/1185 nt) atoB → acetyl‑CoA acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,324,4631.G98.1% 510.6 / 1.4 161coding (335/1185 nt)atoBacetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base . (0/3);  new base G (60/98);  total (60/101)
Fisher's exact test for biased strand distribution p-value = 2.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 224 total aligned reads displayed.

ATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTA  >  NC_000913/2324371‑2324550
                                                                                              |                                                                                        
aTTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGc                                                                                           <  1:7438023/93‑1 (MQ=255)
aTTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGc                                                                                           <  1:2023294/93‑1 (MQ=255)
 ttCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑g                                                                                        >  2:4729840/1‑93 (MQ=255)
  tCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑gg                                                                                       <  2:8652664/93‑1 (MQ=255)
   cACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑ggg                                                                                      <  2:1579321/93‑1 (MQ=255)
    aCGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑gggg                                                                                     >  1:8217083/1‑93 (MQ=255)
    aCGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑gggg                                                                                     <  1:4720112/93‑1 (MQ=255)
     cGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑ggggg                                                                                    >  1:5814843/1‑93 (MQ=255)
      ggTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑gggggg                                                                                   >  2:9426625/1‑93 (MQ=255)
      ggTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑gggggg                                                                                   <  1:6683123/93‑1 (MQ=255)
      ggTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑gggggg                                                                                   <  2:7715024/93‑1 (MQ=255)
      ggTCAATAAAGTATGTGGTGCGGGTCTTAAAAGTGCGGCGCTTCCCGCCCCGGGCATTCCGGCAGGTCAGGCGCGGAGCATT‑GTGgg‑‑gggggg                                                                                   <  2:2228566/93‑1 (MQ=255)
       gTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑ggggggg                                                                                  >  1:2371363/1‑92 (MQ=255)
        tCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑gggggggt                                                                                 <  2:8371202/93‑3 (MQ=255)
         cAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑gggggggta                                                                                <  2:6105842/93‑4 (MQ=255)
          aaTAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑gggggggtat                                                                               <  1:1398194/93‑5 (MQ=255)
          aaTAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑gggggggggt                                                                               >  2:432669/1‑89 (MQ=255)
          aaTAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑ggggggggat                                                                               >  1:4948582/1‑89 (MQ=255)
            tAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATgg                                                                              <  1:6338074/93‑1 (MQ=255)
                  aTGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAata                                                                        <  1:103050/93‑1 (MQ=255)
                   tgtgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAatat                                                                       <  2:7411658/93‑1 (MQ=255)
                    gtgGTTCGGGTCTTGAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGGAAGGAAAATATg                                                                      >  2:3031161/1‑93 (MQ=255)
                     tgGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGa                                                                     <  1:9304483/93‑1 (MQ=255)
                       gTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGt                                                                   <  2:3728020/93‑1 (MQ=255)
                        ttCTGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGtt                                                                  <  1:8013827/93‑1 (MQ=255)
                        ttCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGtt                                                                  <  1:3952994/93‑1 (MQ=255)
                         tCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGttt                                                                 <  2:1426909/93‑1 (MQ=255)
                         tCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGttt                                                                 <  1:6084676/93‑1 (MQ=255)
                         tCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGttt                                                                 <  1:8962484/93‑1 (MQ=255)
                           gggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGGATGGAAAATATGAGGTTAg                                                               >  1:5522618/1‑93 (MQ=255)
                             gTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGcc                                                             <  1:4299412/93‑1 (MQ=255)
                             gTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGGATGGAAAAAATGAGTTTAGcc                                                             >  1:8355174/1‑93 (MQ=255)
                              tCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGccc                                                            <  2:2792401/93‑1 (MQ=255)
                               cTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGGATGGGAAATATGAGTTTAGgccc                                                           >  1:3100082/1‑93 (MQ=255)
                                  agaaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCta                                                        <  1:515760/91‑1 (MQ=255)
                                  aaaaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCta                                                        <  1:3244963/93‑1 (MQ=255)
                                  aaaaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGGCTGGAAAATATGAGTTTAGCCCCCtc                                                        >  2:7005582/1‑92 (MQ=255)
                                  aaaaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGGATGGAAAAAATGAGTTTAGccccccc                                                        >  1:2965111/1‑91 (MQ=255)
                                   aaaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCtac                                                       <  2:5377955/93‑1 (MQ=255)
                                   aaaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGGATGGGAAATATGAGTTTAGGCCCCtac                                                       >  2:7946926/1‑93 (MQ=255)
                                    aaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCtact                                                      <  1:8704033/93‑1 (MQ=255)
                                      gtgtGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGGATGGAAAATATGAGGTTAGCCCCCtactta                                                    >  2:8865281/1‑93 (MQ=255)
                                           gcgcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGa                                               <  2:3865781/93‑1 (MQ=255)
                                             gcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGGATGGGAAATATGAGTTTAGCCCCCTACTTACTCGATg                                             >  1:4082496/1‑93 (MQ=255)
                                             gcTTGCCCCCCAGGCCATTCAGGCAGGGCAGGCGCGGAGCATTGGTGCG‑‑GGGGGGTATGGAAAAGATGAGTTTAGCCCCCTACTTACTCGATg                                             <  1:7238210/93‑1 (MQ=255)
                                              cTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGc                                            >  2:8180777/1‑93 (MQ=255)
                                              cTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGGATGGAAAAAATGAGTTTAGCCCCCTACTTACTCGATGc                                            >  2:7321299/1‑93 (MQ=255)
                                               ttGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑GGGGGGGATGGGAAAAATGAGATTAGGCCCCTACTTTCTCGATGCaa                                          >  1:3790280/1‑93 (MQ=255)
                                                tGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCaa                                          <  1:1710317/93‑1 (MQ=255)
                                                  ccgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGGATGGGAAATATGAGGTTAGCCCCCTACTTACTCGATGCaaaa                                        >  1:686021/1‑93 (MQ=255)
                                                    gccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAga                                      >  2:5470151/1‑92 (MQ=255)
                                                     cccAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCa                                     <  2:6938488/93‑1 (MQ=255)
                                                     cccAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCa                                     <  1:10009915/93‑1 (MQ=255)
                                                      ccAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCAc                                    <  1:8855557/93‑1 (MQ=255)
                                                      ccAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCAc                                    <  1:7740808/93‑1 (MQ=255)
                                                        aGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGc                                  <  1:2997805/93‑1 (MQ=255)
                                                        aGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGc                                  >  2:6510418/1‑93 (MQ=255)
                                                        aGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGGATGGAAAATATGAGGTTAGCCCCCTACTTACTCGATGCAAAAGCACGc                                  >  2:1777717/1‑93 (MQ=255)
                                                          gCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGACG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGctc                                <  1:9712512/93‑1 (MQ=255)
                                                           ccATTCAGGCAGGTCAGGCGCAGCGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGctct                               <  2:1964972/93‑1 (MQ=255)
                                                           ccATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGctct                               <  1:538398/93‑1 (MQ=255)
                                                             aTTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTgg                             <  2:4825859/93‑1 (MQ=255)
                                                             aTTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTgg                             >  1:7618812/1‑93 (MQ=255)
                                                             aTTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTgg                             >  2:3660378/1‑93 (MQ=255)
                                                             aTTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTgg                             >  1:354702/1‑93 (MQ=255)
                                                              ttCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGt                            <  1:2079716/93‑1 (MQ=255)
                                                              ttCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGt                            >  2:9338800/1‑93 (MQ=255)
                                                               tCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGtt                           <  2:1187749/93‑1 (MQ=255)
                                                               tCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGGACGCTCTggat                           >  2:168529/1‑91 (MQ=255)
                                                                caggcaggTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTa                          <  2:1742358/93‑1 (MQ=255)
                                                                caggcaggTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTa                          <  1:9097644/93‑1 (MQ=255)
                                                                  ggcaggTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATc                        >  1:6581189/1‑93 (MQ=255)
                                                                   gcaggTCAGGCGCAGAACATT‑GTGGCG‑GGGGGGGATGGGAAAAATTAGTTTTGCCCCCCACTTTCTCGATGCAAAAAAACGCTCTGGTTTTCg                       >  2:5816094/1‑93 (MQ=255)
                                                                    caggTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGt                      <  2:2852166/93‑1 (MQ=255)
                                                                      ggTCAGGCGCAGAGCATT‑GTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCt                    >  2:5771946/1‑93 (MQ=255)
                                                                         cAGGCGCAGAGCATT‑GTGGCGGGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTg                  <  1:1096691/93‑1 (MQ=255)
                                                                         cAGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTgg                 <  1:6179084/93‑1 (MQ=255)
                                                                          aGGCGCGTAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGga                <  2:7275167/93‑1 (MQ=255)
                                                                          aGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGga                <  2:9016053/93‑1 (MQ=255)
                                                                          aGGCGCAGAGCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGga                <  2:6602786/93‑1 (MQ=255)
                                                                          aGGCGCAGAGCATT‑GTGGCG‑GGGGGGCATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGga                >  2:9703763/1‑93 (MQ=255)
                                                                            gcgcAGAGCATT‑GTGGCG‑GGGGGGTATGCAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGgaga              <  1:717229/93‑1 (MQ=255)
                                                                               cAGAGCATT‑GTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGGCgg           >  2:9273011/1‑93 (MQ=255)
                                                                                agagCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGa          <  2:5967287/93‑1 (MQ=255)
                                                                                agagCATT‑GTGGCG‑GGGGGGGATGGAGAAAATGAGTTTAGGCCCCTACTTACTCGATGTCAATACAAGCTCTGGTTATCGTCTTGGAGACCGa          >  2:9418727/1‑93 (MQ=255)
                                                                                agagCATT‑GTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGa          >  1:6540269/1‑93 (MQ=255)
                                                                                 gagCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACgggc         >  2:1252119/1‑91 (MQ=255)
                                                                                 gagCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAc         <  1:260595/93‑1 (MQ=255)
                                                                                 gagCATT‑GTGGCG‑GGGGGGGATGGGAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAc         >  1:5594673/1‑93 (MQ=255)
                                                                                 gagCATT‑GTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAc         >  2:4460605/1‑93 (MQ=255)
                                                                                 gagCATT‑GTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGGCTTGGAGACggga         >  2:5688044/1‑91 (MQ=255)
                                                                                  agCATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACa        <  2:7608425/93‑1 (MQ=255)
                                                                                  agCATT‑GTGGCG‑GGGGGGCGTGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCACAAGCACGCTCCGGTTATCGTCTTCGACGCGGACa        >  2:4469717/1‑93 (MQ=255)
                                                                                   gCATT‑GTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTAGTTATCGTCTTTGAGACGGACAg       >  2:5517945/1‑93 (MQ=255)
                                                                                   acATT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAg       <  1:1844351/92‑1 (MQ=255)
                                                                                     aTT‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGt     <  1:8120354/93‑1 (MQ=255)
                                                                                      tt‑GTGGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGtt    <  1:9953500/93‑1 (MQ=255)
                                                                                      tt‑GTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTTGAGACGGACAGGtt    >  2:4888739/1‑93 (MQ=255)
                                                                                      tt‑GTGGCG‑GGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGtt    >  2:3336923/1‑93 (MQ=255)
                                                                                         gtgGCG‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTa  <  1:1239166/93‑1 (MQ=255)
                                                                                              |                                                                                        
ATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑GGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTA  >  NC_000913/2324371‑2324550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCT  >  NC_000913/2324377‑2324562
                                                                                                |                                                                                              
GGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑GG‑GGGG‑G                                                                                              >  SRR4302540.164602/1‑94 (MQ=60)
GGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑GG‑GGGG‑G                                                                                              >  SRR4302540.2396913/1‑94 (MQ=60)
GGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑GG‑GGGG‑G                                                                                              <  SRR4302540.6756937/94‑1 (MQ=60)
 GTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑GG‑GGGGgt                                                                                              <  SRR4302540.6135348/94‑3 (MQ=60)
  TCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑GG‑GGGGgga                                                                                             >  SRR4302540.398286/1‑91 (MQ=60)
   CAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑GG‑GGGGggat                                                                                            >  SRR4302540.5002032/1‑90 (MQ=60)
    AATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATG                                                                                           <  SRR4302540.1413210/94‑1 (MQ=60)
     ATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGG                                                                                          <  SRR4302540.6413778/94‑1 (MQ=60)
     ATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑GG‑GGGGggatgg                                                                                          >  SRR4302540.6912178/1‑88 (MQ=60)
     ATAAAGTATGTGGTTCGGGTCTTAAAAGTGCGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑GG‑GGGGggatgg                                                                                          >  SRR4302540.5098218/1‑88 (MQ=60)
      TAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGA                                                                                         <  SRR4302540.6408658/94‑1 (MQ=60)
      TAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑GG‑GGGGgggtgga                                                                                         >  SRR4302540.6733570/1‑87 (MQ=60)
          GTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑GG‑GGGGggatggaaaaa                                                                                     >  SRR4302540.1071711/1‑83 (MQ=60)
           TATGTGGTTCGGGTCTTAAAAGTGTGGCGCCTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑GG‑GGGGggagtgaaaaat                                                                                    >  SRR4302540.9001064/1‑82 (MQ=60)
            ATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATAT                                                                                   <  SRR4302540.104150/94‑1 (MQ=60)
            ATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATAT                                                                                   <  SRR4302540.6537205/94‑1 (MQ=60)
            ATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAAAAT                                                                                   >  SRR4302540.6165058/1‑94 (MQ=60)
            ATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGAAAATAT                                                                                   >  SRR4302540.6688287/1‑94 (MQ=60)
            ATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑GG‑GGGGggagtgaaaatat                                                                                   >  SRR4302540.8401699/1‑81 (MQ=60)
               TGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAG                                                                                <  SRR4302540.9415611/94‑1 (MQ=60)
               TGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAG                                                                                <  SRR4302540.2717566/94‑1 (MQ=60)
                 GTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGAAAATATGAGTT                                                                              >  SRR4302540.8311722/1‑94 (MQ=60)
                  TTCTGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTT                                                                             <  SRR4302540.8107041/94‑1 (MQ=60)
                  TTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTT                                                                             <  SRR4302540.3995651/94‑1 (MQ=60)
                   TCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTA                                                                            <  SRR4302540.2241718/94‑1 (MQ=60)
                   TCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTA                                                                            <  SRR4302540.6152390/94‑1 (MQ=60)
                   TCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTA                                                                            <  SRR4302540.923167/94‑1 (MQ=60)
                   TCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTA                                                                            <  SRR4302540.639388/94‑1 (MQ=60)
                   TCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTA                                                                            <  SRR4302540.9068568/94‑1 (MQ=60)
                    CGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAG                                                                           <  SRR4302540.3664184/94‑1 (MQ=60)
                    CGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGAAAATATGAGGTTAG                                                                           >  SRR4302540.5583430/1‑94 (MQ=60)
                      GGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGAAAAAATGAGTTTAGCC                                                                         >  SRR4302540.8452146/1‑94 (MQ=60)
                       GTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCC                                                                        <  SRR4302540.4346209/94‑1 (MQ=60)
                        TCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGGAAATATGAGTTTAGGCCC                                                                       >  SRR4302540.3134042/1‑94 (MQ=60)
                        TCTTAAAAGTGTGGCGCTTCCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCC                                                                       <  SRR4302540.6617820/94‑1 (MQ=60)
                         CTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCGGGG‑GGGG‑GATGGAAAAAATGAGTTTAGCCCC                                                                       >  SRR4302540.1409052/1‑94 (MQ=60)
                          TTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCT                                                                     <  SRR4302540.8791168/94‑1 (MQ=60)
                           TAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGAAAAAATGAGTTTAGCCCCCcc                                                                    >  SRR4302540.2997830/1‑92 (MQ=60)
                            AGAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTAC                                                                   <  SRR4302540.521290/94‑1 (MQ=60)
                            AAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTAC                                                                   <  SRR4302540.9122153/94‑1 (MQ=60)
                            AAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTAC                                                                   <  SRR4302540.3280290/94‑1 (MQ=60)
                             aaagttgtcgtcgTGCCGCCGAGGCCATTCAGGCAGGGCAGGCGCAGAGCAGT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACT                                                                  <  SRR4302540.8781190/81‑1 (MQ=60)
                             AAAGTGTGGCTGTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑GG‑GGGGTTATGGAAAATATGAGTTTAGCCCCCTACT                                                                  <  SRR4302540.5549207/94‑1 (MQ=60)
                             AAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACT                                                                  <  SRR4302540.3024004/94‑1 (MQ=60)
                             AAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACT                                                                  <  SRR4302540.426168/94‑1 (MQ=60)
                              AAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTT                                                                 <  SRR4302540.8805068/94‑1 (MQ=60)
                               AGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGGAAATATGAGGTTAGGCCCCTACTTC                                                                >  SRR4302540.661991/1‑94 (MQ=60)
                               AGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑GG‑GGGGgtgggggaaacacgggttgcggcccctactta                                                                >  SRR4302540.2181942/1‑62 (MQ=60)
                                GTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTAC                                                               <  SRR4302540.3923067/94‑1 (MQ=60)
                                   TGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCG                                                            <  SRR4302540.9166051/94‑1 (MQ=60)
                                      CGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGGAAATATGAGTTTAGCCCCCTACTTACTCGATG                                                         >  SRR4302540.4126515/1‑94 (MQ=60)
                                      CGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATG                                                         >  SRR4302540.774360/1‑94 (MQ=60)
                                      CGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATG                                                         >  SRR4302540.4126514/1‑94 (MQ=60)
                                       GCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGC                                                        <  SRR4302540.8410631/94‑1 (MQ=60)
                                       GCTTGCCCCCCAGGCCATTCAGGCAGGGCAGGCGCGGAGCATTGGTGCG‑‑GG‑GGGG‑TATGGAAAAGATGAGTTTAGCCCCCTACTTACTCGATGC                                                        <  SRR4302540.7318941/94‑1 (MQ=60)
                                        CTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCA                                                       <  SRR4302540.5315966/94‑1 (MQ=60)
                                        CTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑GG‑GGGG‑GATGGGAAAAATGAGATTAGGCCCCTACTTTCTCGATGCAA                                                      >  SRR4302540.3831603/1‑94 (MQ=60)
                                         TTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGGAAATATGAGTTTAGCCCCCTACTTACTCGATGCAA                                                      >  SRR4302540.2308138/1‑94 (MQ=60)
                                         TTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAA                                                      >  SRR4302540.812346/1‑94 (MQ=60)
                                          TGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAA                                                     <  SRR4302540.1728796/94‑1 (MQ=60)
                                           GCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGGAAATATGAGGTTAGCCCCCTACTTACTCGATGCAAAA                                                    >  SRR4302540.693382/1‑94 (MQ=60)
                                            CCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG                                                   <  SRR4302540.7431921/94‑1 (MQ=60)
                                               CCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCAC                                                <  SRR4302540.10126473/94‑1 (MQ=60)
                                               CCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGAAAATATTAGTTTAGCCCCCTACTTACTCGATGCAAAAGCAC                                                >  SRR4302540.7666007/1‑94 (MQ=60)
                                               CCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCAC                                                >  SRR4302540.304273/1‑94 (MQ=60)
                                               CCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGAAAATATGAGGTTAGCCCCCCACTTACTCGATGCAAAAGGAC                                                >  SRR4302540.499923/1‑94 (MQ=60)
                                                CCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACG                                               <  SRR4302540.3149067/94‑1 (MQ=60)
                                                CCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACG                                               <  SRR4302540.7831180/94‑1 (MQ=60)
                                                CCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACG                                               <  SRR4302540.8958016/94‑1 (MQ=60)
                                                CCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGAAAATATGAGGTTAGCCCCCCCCTTACTCGCTGCAAAAacacc                                               >  SRR4302540.1778810/1‑89 (MQ=60)
                                                CCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑GG‑GGGGgggagggaaaaaatagggtatgccccgtacttccctgacaccaaacagc                                               >  SRR4302540.1778531/1‑45 (MQ=60)
                                                 CAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TGTGGGAAATATGAGTTTAGCCCCCTTCTTACTCGATGCAAAAGCACGC                                              >  SRR4302540.4443981/1‑94 (MQ=60)
                                                  AGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCT                                             <  SRR4302540.3030835/94‑1 (MQ=60)
                                                    GCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGACG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCT                                           <  SRR4302540.9826654/94‑1 (MQ=60)
                                                     CCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTG                                          <  SRR4302540.7877646/94‑1 (MQ=60)
                                                     CCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTG                                          <  SRR4302540.544222/94‑1 (MQ=60)
                                                     CCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTG                                          <  SRR4302540.357653/94‑1 (MQ=60)
                                                      CATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGG                                         >  SRR4302540.7704478/1‑94 (MQ=60)
                                                      CATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGG                                         >  SRR4302540.358341/1‑94 (MQ=60)
                                                      CATTCAGGCAGGGCAGGCGCAGAGCATT‑GTGGC‑‑GGTGGGG‑GGTGGGAAATATGATTTTAGGACCCTACTTACTCGATTCAAAAACACGCcctcg                                         >  SRR4302540.358342/1‑89 (MQ=28)
                                                       ATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGAAAAAATGAGTTTAGCCCCCCACTTACTCGATGCAAAAGCACGCTCTTGT                                        >  SRR4302540.6893948/1‑94 (MQ=60)
                                                        TTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATTGAAAATATGAGGTTAGCCCCCTAGTTACTCTATGCACAAGCACGCTCTGGTT                                       >  SRR4302540.5725924/1‑94 (MQ=60)
                                                        TTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTT                                       <  SRR4302540.2102063/94‑1 (MQ=60)
                                                        TTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTT                                       <  SRR4302540.6531358/94‑1 (MQ=60)
                                                          CAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATTGTAAAAATGAGTTTATCCCCCTTTTTACTCGATTAAAAAGCACGCTTTCGTTAT                                     >  SRR4302540.6968407/1‑94 (MQ=30)
                                                          CAGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTAT                                     <  SRR4302540.9206953/94‑1 (MQ=60)
                                                           AGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC                                    <  SRR4302540.1543659/94‑1 (MQ=60)
                                                           AGGCAGGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATC                                    >  SRR4302540.6654089/1‑94 (MQ=60)
                                                                GGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTT                               <  SRR4302540.1109950/94‑1 (MQ=60)
                                                                GGTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTT                               <  SRR4302540.9960875/94‑1 (MQ=60)
                                                                 GTCAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTG                              <  SRR4302540.5616548/94‑1 (MQ=60)
                                                                 GTCAGGCGCAGAGCAGT‑GTGGCG‑GG‑GGGG‑GATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTG                              <  SRR4302540.7227326/94‑1 (MQ=60)
                                                                   CAGGCGCAGAGCATT‑GTGGCGGGG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGG                             <  SRR4302540.1107979/94‑1 (MQ=60)
                                                                   CAGGCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGA                            <  SRR4302540.6247918/94‑1 (MQ=60)
                                                                      GCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGAC                         <  SRR4302540.8972201/94‑1 (MQ=60)
                                                                      GCGCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGCAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGAC                         <  SRR4302540.724923/94‑1 (MQ=60)
                                                                        GCAGAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGG                       <  SRR4302540.7295623/94‑1 (MQ=60)
                                                                         CAGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGGCGGA                      >  SRR4302540.9764065/1‑94 (MQ=60)
                                                                         CAGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGA                      >  SRR4302540.6612849/1‑94 (MQ=60)
                                                                          AGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGGAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAC                     >  SRR4302540.5656229/1‑94 (MQ=60)
                                                                          AGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAC                     >  SRR4302540.5656228/1‑94 (MQ=60)
                                                                          AGAGCATT‑GTGGCG‑GG‑GGGG‑GATGGAAAATATGAGGTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGgcggga                     >  SRR4302540.5036174/1‑88 (MQ=60)
                                                                           GAGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACA                    <  SRR4302540.263135/94‑1 (MQ=60)
                                                                            AGCATT‑GTGGCG‑GG‑GGGG‑TATGGGAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAG                   >  SRR4302540.207047/1‑94 (MQ=60)
                                                                            AGCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAG                   <  SRR4302540.8847693/94‑1 (MQ=60)
                                                                             GCATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGG                  <  SRR4302540.7714786/94‑1 (MQ=60)
                                                                             ACATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGG                  <  SRR4302540.1864559/94‑1 (MQ=60)
                                                                               ATT‑GTGGCG‑GG‑GGGG‑TATGTAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTT                <  SRR4302540.2509614/94‑1 (MQ=60)
                                                                               ATT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTT                <  SRR4302540.8214593/94‑1 (MQ=60)
                                                                               ATT‑GTGGCG‑GG‑GGGG‑GATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTT                >  SRR4302540.8443273/1‑94 (MQ=60)
                                                                                TT‑GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTT               <  SRR4302540.10069688/94‑1 (MQ=60)
                                                                                   GTGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTAT             <  SRR4302540.1251816/94‑1 (MQ=60)
                                                                                    TGGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATG            <  SRR4302540.9297478/94‑1 (MQ=60)
                                                                                     GGCG‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGA           <  SRR4302540.1862495/94‑1 (MQ=60)
                                                                                       gcgGG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGAC          >  SRR4302540.3186297/4‑94 (MQ=60)
                                                                                       G‑‑GG‑GGGG‑TATGGACAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACCT        <  SRR4302540.9073642/94‑1 (MQ=60)
                                                                                        cgGG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACG         >  SRR4302540.4067629/3‑94 (MQ=60)
                                                                                             GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAAT     >  SRR4302540.6258387/1‑94 (MQ=60)
                                                                                             GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGGTTATGACGTAAT     >  SRR4302540.8627330/1‑94 (MQ=60)
                                                                                              GGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATC    <  SRR4302540.7363597/94‑1 (MQ=60)
                                                                                               GG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCC   >  SRR4302540.6319938/1‑94 (MQ=60)
                                                                                               GG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCC   >  SRR4302540.6325877/1‑94 (MQ=60)
                                                                                               GG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCC   <  SRR4302540.5221908/94‑1 (MQ=60)
                                                                                                G‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCT  >  SRR4302540.8393674/1‑94 (MQ=60)
                                                                                                G‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCT  >  SRR4302540.8926858/1‑94 (MQ=60)
                                                                                                G‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCT  >  SRR4302540.2069078/1‑94 (MQ=60)
                                                                                                G‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCT  <  SRR4302540.8714267/94‑1 (MQ=60)
                                                                                                G‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTCATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCT  <  SRR4302540.4709876/94‑1 (MQ=60)
                                                                                                |                                                                                              
GGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATT‑GTGGC‑‑GG‑GGGG‑TATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCT  >  NC_000913/2324377‑2324562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑