Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,324,469 (G)6→7 coding (341/1185 nt) atoB → acetyl‑CoA acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,324,4631.G98.6% 438.6 / ‑1.1 138coding (335/1185 nt)atoBacetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base G (54/82);  total (55/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 196 total aligned reads displayed.

ATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTA  >  NC_000913/2324371‑2324550
                                                                                             |                                                                                         
  tCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑gg                                                                                         >  1:5549605/1‑93 (MQ=255)
  tCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑gg                                                                                         <  1:1143576/93‑1 (MQ=255)
  tCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑gg                                                                                         <  1:4081043/93‑1 (MQ=255)
   cACGGTCAATAAAGTATGTGGTTCGGTTCTTAAAAGTGTGGCGCTAGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggg                                                                                        <  2:1912871/93‑1 (MQ=255)
   cACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggg                                                                                        >  2:5891559/1‑93 (MQ=255)
   cACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggg                                                                                        <  1:2633967/93‑1 (MQ=255)
   cACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggg                                                                                        <  1:1919526/93‑1 (MQ=255)
    aCGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑gggg                                                                                       <  1:1051799/93‑1 (MQ=255)
    aCGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑gggg                                                                                       <  1:5349260/93‑1 (MQ=255)
    aCGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑gggg                                                                                       <  2:629667/93‑1 (MQ=255)
      ggTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggggg‑g                                                                                    <  2:406951/93‑1 (MQ=255)
      ggTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggggg‑g                                                                                    <  2:6041644/93‑1 (MQ=255)
      ggTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggggg‑g                                                                                    >  1:4013842/1‑93 (MQ=255)
       gTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggggg‑gg                                                                                   >  1:5900872/1‑92 (MQ=255)
        tCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggggg‑ggt                                                                                  <  1:932321/93‑3 (MQ=255)
        tCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggggg‑ggt                                                                                  <  1:4275363/93‑3 (MQ=255)
        tCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggggg‑ggg                                                                                  >  1:2115407/1‑91 (MQ=255)
         cAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggggg‑gggg                                                                                 >  1:5438507/1‑90 (MQ=255)
          aaTAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑ggggg‑ggggt                                                                                >  1:3859485/1‑89 (MQ=255)
               aGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGaaa                                                                           >  2:1414358/1‑93 (MQ=255)
                gTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGGGG‑GGATTGGaaaa                                                                          >  1:2924870/1‑93 (MQ=255)
                 tATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGaaaaa                                                                         >  2:3670633/1‑92 (MQ=255)
                 tATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGAAAat                                                                         >  1:4264375/1‑93 (MQ=255)
                  aTGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAata                                                                        <  1:3051352/93‑1 (MQ=255)
                  aTGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAata                                                                        <  2:4929864/93‑1 (MQ=255)
                  aTGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGGAAata                                                                        >  2:840366/1‑93 (MQ=255)
                  aTGTGGTTCGCGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAata                                                                        <  1:775569/93‑1 (MQ=255)
                         tCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGttt                                                                 <  1:5508003/93‑1 (MQ=255)
                         tCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGttt                                                                 <  2:1043290/93‑1 (MQ=255)
                         tCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGttt                                                                 <  1:1597540/93‑1 (MQ=255)
                         tCGGGTCTTAAAAGCGTGGCGCTTGCCGCGCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGttt                                                                 <  2:1395367/93‑1 (MQ=255)
                          cGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGAAAATATGAGTTTa                                                                >  1:5845938/1‑93 (MQ=255)
                           gggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGG‑TGGAAAATATTAGGTTAg                                                               >  2:1237145/1‑93 (MQ=255)
                           gggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGGAAAAATGAGGTTaa                                                               >  2:3288695/1‑92 (MQ=255)
                            ggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGc                                                              <  2:3118317/93‑1 (MQ=255)
                              tCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGG‑TGGGAAATATGAGGTTAGcac                                                            >  2:3581210/1‑91 (MQ=255)
                              tCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGAAAATATGAGTTTAGccc                                                            >  2:4663305/1‑93 (MQ=255)
                               cTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGAAAATATGAGGTTAGgccc                                                           >  1:2159742/1‑93 (MQ=255)
                                ttAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGccccc                                                          <  2:446199/93‑1 (MQ=255)
                                ttAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGccccc                                                          <  2:5301796/93‑1 (MQ=255)
                                ttAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGGAAAAATGAGGTTAGacccc                                                          >  2:4324909/1‑93 (MQ=255)
                                 tAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCt                                                         <  2:5413966/93‑1 (MQ=255)
                                  aaaaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGGAAATATGAGGTTAGCCCCCta                                                        >  1:659775/1‑93 (MQ=255)
                                  aaaaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGAAAATATGAGTTTAGGCCCCtc                                                        >  1:1066990/1‑92 (MQ=255)
                                    aaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGAAAATATGAGGTTAGCCCCCtact                                                      >  1:897301/1‑93 (MQ=255)
                                     aGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGGAAATATGAGTTTAGCCCCCtactt                                                     >  1:4132239/1‑93 (MQ=255)
                                     aGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGAAAATATGAGTTTAGCCCCCtactt                                                     >  2:1940863/1‑93 (MQ=255)
                                      gtgtGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCCTTGTGGCGGGGGG‑GGA‑TGGAAAATATGAGATTAGCCCCCtactta                                                    >  1:4480168/1‑93 (MQ=255)
                                        gtgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCtacttact                                                  <  2:2534278/93‑1 (MQ=255)
                                        gtgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCtacttact                                                  <  2:4998824/93‑1 (MQ=255)
                                          ggCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCg                                                >  2:1993721/1‑93 (MQ=255)
                                           gcgcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGa                                               <  1:5630166/93‑1 (MQ=255)
                                             gcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATg                                             >  2:4838920/1‑93 (MQ=255)
                                             gcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATg                                             <  1:3343696/93‑1 (MQ=255)
                                             gcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGGGGCGGA‑TGGAAAATATGAGTTTAGCCCCCTTCTTACTCGAtt                                             >  1:5321240/1‑92 (MQ=255)
                                              cTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGc                                            <  2:146009/93‑1 (MQ=255)
                                              cTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGc                                            <  2:2569810/93‑1 (MQ=255)
                                              cTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGGAAATATGAGTTTAGCCCCCTACTTACTCGATGc                                            >  1:5659413/1‑93 (MQ=255)
                                              cTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGAAAATATGAGGTTAGGCCCCTACTTACTCCATGc                                            >  1:363229/1‑93 (MQ=255)
                                               ttGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGGAAATATGAGTTTAGCCCCCTACTTACTCGATGCa                                           >  1:4249200/1‑93 (MQ=255)
                                                 gccgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGGAAATATGAGTTTAGCCCCCTACTTACTCGATGCaaa                                         >  2:27816/1‑93 (MQ=255)
                                                  ccgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCaaaa                                        <  1:1548395/93‑1 (MQ=255)
                                                  ccgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCaaaa                                        <  2:4026745/93‑1 (MQ=255)
                                                   cgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAg                                       <  1:5602327/93‑1 (MQ=255)
                                                    gccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGAAAATATGAGTTTAGGCCCCTACTTACTCGATGCaaaaac                                      >  2:5163397/1‑91 (MQ=255)
                                                    gccCAGGCCATACAGGCAGAACAGGCACAGAGCATGGTGGC‑GGGGGAGTA‑TGAAAAATATGAGTTTAGCCCAATACTTTCTCGTTGCAAAAGc                                      <  2:892068/93‑1 (MQ=255)
                                                     cccAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCa                                     <  1:5025802/93‑1 (MQ=255)
                                                      ccAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCAc                                    <  1:1382227/93‑1 (MQ=255)
                                                       cAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACg                                   >  2:2257320/1‑93 (MQ=255)
                                                       cAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAACACg                                   >  2:1286534/1‑93 (MQ=255)
                                                       cAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGAAAATATGAGGTTAGCCCCCTACTTAATCGATGCAAAAACaac                                   >  1:2195172/1‑91 (MQ=255)
                                                        aGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGGAAATATGAGTTTAGCACCCTACTTACTCGATGCAAAAGCACGc                                  >  1:4372660/1‑93 (MQ=255)
                                                         ggCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGGAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGct                                 >  2:612893/1‑93 (MQ=255)
                                                         ggCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGGAAATATGAGGTTAGCCCCCTACTTACTCGATGCAAAAGCACGcc                                 >  2:453687/1‑92 (MQ=255)
                                                         ggCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGGAAATATGAGGTTAGCCCCCTACTTACTCGATGCAAAAGAACGcc                                 >  2:1114972/1‑92 (MQ=255)
                                                           ccATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGctct                               <  1:6017229/93‑1 (MQ=255)
                                                            cATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTg                              <  1:6214256/93‑1 (MQ=255)
                                                            cATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTg                              <  1:3460161/93‑1 (MQ=255)
                                                            cATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTg                              <  2:1697055/93‑1 (MQ=255)
                                                             aTTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTgg                             <  1:4973387/93‑1 (MQ=255)
                                                              ttCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGt                            <  2:3272930/93‑1 (MQ=255)
                                                              ttCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGGACGCTCTTGt                            >  2:6174735/1‑93 (MQ=255)
                                                                  ggcaggTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGAAAATATGAGGTTAGCCCCCTACTTACTCGATGCAAAAACACGCTCTGGTTATc                        >  2:3869913/1‑93 (MQ=255)
                                                                   gcaggTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCg                       <  1:5984204/93‑1 (MQ=255)
                                                                     aggTCAGGCGCAGAGCATTGTGGCGGGGGG‑GGA‑TGGGAAATATGGGTTTAGCCCCCTTCTTACTCGATGCAAAAGCAAGCTCTGGTTTTCggt                     >  2:2065746/1‑91 (MQ=255)
                                                                       gTCAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCtt                   <  1:4740624/93‑1 (MQ=255)
                                                                         cAGGCGCAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTgg                 <  2:4135362/93‑1 (MQ=255)
                                                                            gcgcAGAGCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGgaga              <  2:1609453/93‑1 (MQ=255)
                                                                            gcgcAGAGCATTGTGGCGGGGGG‑GGA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGgaga              >  2:444904/1‑93 (MQ=255)
                                                                             cgcAGAGCATTGTGGCGGGGGG‑GGA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCCAAAGCACGCTCTGGTTATCGTCTTGGAGAc             >  2:683765/1‑93 (MQ=255)
                                                                              gcAGAGCATTGTGGCGGGGGG‑GGA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACg            >  2:5774290/1‑93 (MQ=255)
                                                                                agagCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGa          <  1:178448/93‑1 (MQ=255)
                                                                                agagCATTGTGGCGGGGGG‑GGA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCCATGCAAAAGCACGCTCTTGTTATCGTCTTGGAGACGGa          >  2:3356023/1‑93 (MQ=255)
                                                                                 gagCATTGTGGCGGGGGG‑GGA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAc         >  2:5976874/1‑93 (MQ=255)
                                                                                  agCATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACa        <  1:1484562/93‑1 (MQ=255)
                                                                                    cATTGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAgg      <  2:188603/93‑1 (MQ=255)
                                                                                     aTTGTGGCGGGGGG‑GGA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGt     >  1:4605976/1‑93 (MQ=255)
                                                                                      ttGTGGCGGGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGtt    <  2:2347625/93‑1 (MQ=255)
                                                                                      ttGTGGCGGGGGG‑GGA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTTGAGACGGACAgggt    >  1:1179792/1‑91 (MQ=255)
                                                                                      ttGTGGCGGGGGG‑GGA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAgggt    >  1:1624049/1‑91 (MQ=255)
                                                                                             |                                                                                         
ATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑GGGGG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTA  >  NC_000913/2324371‑2324550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCT  >  NC_000913/2324376‑2324562
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CGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑G‑G                                                                                                <  SRR4302533.4264130/94‑1 (MQ=60)
CGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑G‑G                                                                                                >  SRR4302533.4038281/1‑94 (MQ=60)
 GGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑G‑GG                                                                                               >  SRR4302533.5936507/1‑94 (MQ=60)
 GGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑G‑GG                                                                                               <  SRR4302533.3495480/94‑1 (MQ=60)
  GTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑G‑Ggt                                                                                              <  SRR4302533.6317965/94‑3 (MQ=60)
  GTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑G‑Ggg                                                                                              >  SRR4302533.2128453/1‑92 (MQ=60)
   TCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑G‑Ggta                                                                                             <  SRR4302533.937793/94‑4 (MQ=60)
   TCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑G‑Ggta                                                                                             <  SRR4302533.4301081/94‑4 (MQ=60)
   TCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑G‑Gggg                                                                                             >  SRR4302533.5471263/1‑91 (MQ=60)
   TCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTGGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑G‑Ggta                                                                                             <  SRR4302533.945206/94‑4 (MQ=60)
    CAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑G‑Ggggt                                                                                            >  SRR4302533.3883049/1‑90 (MQ=60)
      ATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGG                                                                                          <  SRR4302533.5235185/94‑1 (MQ=60)
          AGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑G‑GGATTGGAAAA                                                                                      >  SRR4302533.2942772/1‑94 (MQ=60)
           GTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GGA‑TGGAAAAT                                                                                     >  SRR4302533.4290060/1‑94 (MQ=60)
           GTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑G‑Gggatgggaaat                                                                                     >  SRR4302533.1723428/1‑83 (MQ=60)
             ATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATAT                                                                                   <  SRR4302533.3070034/94‑1 (MQ=60)
             ATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATAT                                                                                   <  SRR4302533.910363/94‑1 (MQ=60)
             ATGTGGTTCGCGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATAT                                                                                   <  SRR4302533.780126/94‑1 (MQ=60)
              ttgggtttgggttttaaaaggtgggcccttgcccgCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑G‑GTA‑TGGAAAATAT                                                                                   <  SRR4302533.2253562/59‑1 (MQ=60)
              TGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑G‑Ggggtgggaaatatg                                                                                  >  SRR4302533.5429863/1‑80 (MQ=60)
                TGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAG                                                                                <  SRR4302533.3153396/94‑1 (MQ=60)
                 GGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GGA‑TGGGAAATATGAGT                                                                               >  SRR4302533.5818038/1‑94 (MQ=60)
                 GGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GGA‑TGGAAAATATGAGT                                                                               >  SRR4302533.2120474/1‑94 (MQ=60)
                 GGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑G‑Ggggtgggaaatatgagt                                                                               >  SRR4302533.3378947/1‑77 (MQ=60)
                  GTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GGA‑TGGAAAATATGAGTT                                                                              >  SRR4302533.5646387/1‑94 (MQ=60)
                   TTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTT                                                                             <  SRR4302533.5904088/94‑1 (MQ=60)
                    TCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTA                                                                            <  SRR4302533.5541248/94‑1 (MQ=60)
                    TCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTA                                                                            <  SRR4302533.1607213/94‑1 (MQ=60)
                    TCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GGA‑TGGAAAATATGAGTTTA                                                                            >  SRR4302533.5881279/1‑94 (MQ=60)
                      GGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGC                                                                          <  SRR4302533.4320922/94‑1 (MQ=60)
                        GTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCC                                                                        <  SRR4302533.45019/94‑1 (MQ=60)
                        GTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCC                                                                        <  SRR4302533.3038137/94‑1 (MQ=60)
                        GTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCC                                                                        <  SRR4302533.725379/94‑1 (MQ=60)
                        GTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GGA‑TGGGAAATAAGAGTTTAGGCC                                                                        >  SRR4302533.4970944/1‑94 (MQ=60)
                        GTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GGA‑TGGAAAATATGAGGTTTGCCC                                                                        >  SRR4302533.3879363/1‑94 (MQ=60)
                        GTCTTAAAAGTGTGGCGCTTGCCGCCCAGGACATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GGA‑TGGAAAATATGAGTTTAGCCC                                                                        >  SRR4302533.2177665/1‑94 (MQ=60)
                         TCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCC                                                                       <  SRR4302533.1627488/94‑1 (MQ=60)
                         TCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GGA‑TGGAAAATATGAGGTTAGGCCC                                                                       >  SRR4302533.2173038/1‑94 (MQ=60)
                           TTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GGA‑TGGGAAATATGAGTTTAGCCCCCT                                                                     >  SRR4302533.1309942/1‑94 (MQ=60)
                            TAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GGA‑TGGGAAATATGAGGTTAGCCCCCTA                                                                    >  SRR4302533.663663/1‑94 (MQ=60)
                            TAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GGA‑TGGAAAATATGAGTTTAGGCCCCTC                                                                    >  SRR4302533.1073261/1‑94 (MQ=60)
                             AAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GGA‑TGGAAAATATGAGTTTAAGCCCCTAC                                                                   >  SRR4302533.838818/1‑94 (MQ=60)
                              AAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GGA‑TGGAAAATATGAGGTTAGCCCCCTACT                                                                  >  SRR4302533.902568/1‑94 (MQ=60)
                               AAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GGA‑TGGGAAATATGAGTTTAGCCCCCTACTT                                                                 >  SRR4302533.4157325/1‑94 (MQ=60)
                                AGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCCTTGTGGCG‑GGGG‑G‑GGA‑TGGAAAATATGAGATTAGCCCCCTACTTA                                                                >  SRR4302533.4507271/1‑94 (MQ=60)
                                      GCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGAT                                                          <  SRR4302533.5664213/94‑1 (MQ=60)
                                       CGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATG                                                         <  SRR4302533.6042541/94‑1 (MQ=60)
                                       CGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑GCGGA‑TGGAAAATATGAGTTTAGCCCCCTTCTTACTCGATT                                                         >  SRR4302533.5353129/1‑94 (MQ=60)
                                        GCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGC                                                        <  SRR4302533.3364358/94‑1 (MQ=60)
                                        GCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GGA‑TGGGAAATATGAGTTTAGCCCCCTACTTACTCGATGC                                                        >  SRR4302533.5693614/1‑94 (MQ=60)
                                        GCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GGA‑TGGAAAATATGAGGTTAGGCCCCTACTTACTCCATGC                                                        >  SRR4302533.365438/1‑94 (MQ=60)
                                         CTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGGAAATATGAGTTTAGCCCCCTACTTACTCGATGCA                                                       >  SRR4302533.4274843/1‑94 (MQ=60)
                                           TGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAA                                                     <  SRR4302533.1650889/94‑1 (MQ=60)
                                             CCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG                                                   <  SRR4302533.4508178/94‑1 (MQ=60)
                                             CCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG                                                   <  SRR4302533.1540349/94‑1 (MQ=60)
                                             CCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAG                                                   <  SRR4302533.1557726/94‑1 (MQ=60)
                                              CGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGC                                                  <  SRR4302533.5636179/94‑1 (MQ=60)
                                                CCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCAC                                                <  SRR4302533.5056235/94‑1 (MQ=60)
                                                 CCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACG                                               <  SRR4302533.5606552/94‑1 (MQ=60)
                                                 CCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACG                                               <  SRR4302533.1390549/94‑1 (MQ=60)
                                                 CCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GGA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACG                                               >  SRR4302533.5266563/1‑94 (MQ=60)
                                                 CCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GGA‑TGGAAAATATGAGGTTAGCCCCCTACTTAATCGATGCAAAAacaac                                               >  SRR4302533.2208697/1‑89 (MQ=60)
                                                  CAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGC                                              <  SRR4302533.4077933/94‑1 (MQ=60)
                                                  CAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GGG‑TGGGAAATATCAGTTTAGCCCCCTTCTTACTCGGTGCAAAAGCACGC                                              >  SRR4302533.1491608/1‑94 (MQ=60)
                                                  CAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GGA‑TGGGAAATATGAGTTTAGCACCCTACTTACTCGATGCAAAAGCACGC                                              >  SRR4302533.4399054/1‑94 (MQ=60)
                                                   AGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGGGTTTAGCCCCCCACTTAATCGATGCAAAAGAACGCC                                             >  SRR4302533.463628/1‑94 (MQ=60)
                                                      CCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTG                                          <  SRR4302533.6053688/94‑1 (MQ=60)
                                                      CCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GGA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTG                                          >  SRR4302533.5441021/1‑94 (MQ=60)
                                                       CATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGG                                         <  SRR4302533.3890869/94‑1 (MQ=60)
                                                       CATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGG                                         <  SRR4302533.3481520/94‑1 (MQ=60)
                                                       CATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGG                                         <  SRR4302533.1169123/94‑1 (MQ=60)
                                                       CATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGG                                         <  SRR4302533.6251521/94‑1 (MQ=60)
                                                       CATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGG                                         <  SRR4302533.4137450/94‑1 (MQ=60)
                                                        ATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGG‑G‑GT‑‑TGGGAAACATGAGGTTAGCCCCCTACTTACTCGcagtaaaaggacccaatggt                                        >  SRR4302533.4593081/1‑74 (MQ=47)
                                                        ATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGT                                        <  SRR4302533.1562461/94‑1 (MQ=60)
                                                        ATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGT                                        <  SRR4302533.5003543/94‑1 (MQ=60)
                                                              GCAGGTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGT                                  <  SRR4302533.6020446/94‑1 (MQ=60)
                                                                  GTCAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTG                              <  SRR4302533.4769368/94‑1 (MQ=60)
                                                                    CAGGCGCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGA                            <  SRR4302533.5952499/94‑1 (MQ=60)
                                                                       GCGCAGAGCATTGTGGC‑‑GGGGTG‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGAC                         <  SRR4302533.537337/94‑1 (MQ=60)
                                                                        CGCAGAGCATTGTGTCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACG                        <  SRR4302533.5566269/94‑1 (MQ=60)
                                                                         GCAGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGG                       <  SRR4302533.5670269/94‑1 (MQ=60)
                                                                          CAGAGCATTGTGGCG‑GGGG‑G‑GGA‑TGGGAAATATGAGTTTAGCCCCCCACTTACTCGATGCAAAAGCACGCACTGGTTATCGTCTTGGAGgggga                      >  SRR4302533.462966/1‑89 (MQ=60)
                                                                           AGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTTGAGACGGAA                     >  SRR4302533.2811756/1‑94 (MQ=60)
                                                                           AGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAC                     <  SRR4302533.179565/94‑1 (MQ=60)
                                                                           AGAGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGAC                     <  SRR4302533.2154735/94‑1 (MQ=60)
                                                                             AGCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAG                   <  SRR4302533.1493521/94‑1 (MQ=60)
                                                                              GCATTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGG                  <  SRR4302533.1121883/94‑1 (MQ=60)
                                                                               CATTGTGGCG‑GGGG‑G‑GGA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGT                 >  SRR4302533.4633878/1‑94 (MQ=60)
                                                                                ATTGTGGCG‑GGGG‑G‑GGA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTTGAGACGGACAGGGT                >  SRR4302533.1186880/1‑94 (MQ=60)
                                                                                ATTGTGGCG‑GGGG‑G‑GGA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGGT                >  SRR4302533.1633886/1‑94 (MQ=60)
                                                                                 TTGTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTT               <  SRR4302533.270467/94‑1 (MQ=60)
                                                                                   GTGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTAT             <  SRR4302533.5502719/94‑1 (MQ=60)
                                                                                    TGGCG‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATG            <  SRR4302533.3693194/94‑1 (MQ=60)
                                                                                       G‑‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACGTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGT        <  SRR4302533.889530/94‑1 (MQ=60)
                                                                                            GG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGAAGGACAGGGTTATGACGTAAT     >  SRR4302533.1344978/1‑94 (MQ=60)
                                                                                             G‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATC    >  SRR4302533.5654456/1‑94 (MQ=60)
                                                                                               G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCC   <  SRR4302533.6151158/94‑1 (MQ=60)
                                                                                               G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCC   >  SRR4302533.3364902/1‑94 (MQ=60)
                                                                                                 GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCT  >  SRR4302533.6340041/1‑94 (MQ=60)
                                                                                                 GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCT  >  SRR4302533.1152500/1‑94 (MQ=60)
                                                                                                 GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCT  <  SRR4302533.3150856/94‑1 (MQ=60)
                                                                                                 GTA‑GGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCT  <  SRR4302533.1234524/94‑1 (MQ=60)
                                                                                                 |                                                                                              
CGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGC‑‑GGGG‑G‑GTA‑TGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCT  >  NC_000913/2324376‑2324562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑