Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,325,025 (A)5→6 coding (897/1185 nt) atoB → acetyl‑CoA acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,325,0201.A92.0% 638.2 / 41.4 187coding (892/1185 nt)atoBacetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base . (6/9);  new base A (78/94);  total (84/103)
Fisher's exact test for biased strand distribution p-value = 7.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 207 total aligned reads displayed.

CAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGC‑A‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCG  >  NC_000913/2324928‑2325107
                                                                                             |                                                                                         
 agcagGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGC‑a                                                                                          >  2:3694575/1‑93 (MQ=255)
  gcagGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGC‑a‑a                                                                                        >  1:4405239/1‑93 (MQ=255)
     ggCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGC‑a‑aaa‑a                                                                                    <  1:6296013/93‑1 (MQ=255)
        cTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGC‑a‑aaa‑aagc                                                                                 <  1:5716809/93‑4 (MQ=255)
         ttACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGC‑a‑aaa‑aagcg                                                                                >  1:9089051/1‑89 (MQ=255)
         ttACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGC‑a‑aaa‑aagcg                                                                                >  1:4587687/1‑89 (MQ=255)
         ttACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGCATGGGGCCAGTACCTGCCACGC‑a‑aaa‑aagcg                                                                                >  1:5411509/1‑89 (MQ=255)
           aCCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGtt                                                                               <  1:957396/93‑1 (MQ=255)
            ccccccTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTa                                                                              <  1:605691/93‑1 (MQ=255)
              ccccTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACa                                                                            <  1:8575094/93‑1 (MQ=255)
               cccTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACaa                                                                           >  2:3546450/1‑93 (MQ=255)
               cccTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGC‑A‑AAA‑AGCGTTACAAc                                                                          >  2:9488116/1‑93 (MQ=255)
                ccTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAAc                                                                          <  2:9852440/93‑1 (MQ=255)
                ccTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAAc                                                                          <  2:4325263/93‑1 (MQ=255)
                ccTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAAc                                                                          >  1:2066988/1‑93 (MQ=255)
                  tGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTg                                                                        >  2:5754425/1‑93 (MQ=255)
                  tGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTg                                                                        <  2:7641203/93‑1 (MQ=255)
                  cggCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTg                                                                        <  1:8359123/92‑1 (MQ=255)
                   ggCTCGCATTAAAAGTTATGCCAGCGGTGGCGTTCCCCCCGCATTGATGGGTATGGGGCCAGTTCCTGCCACGCAA‑AAA‑AGCGTTACAACTgg                                                                       <  2:127961/93‑1 (MQ=255)
                   ggCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTgg                                                                       <  1:7351777/93‑1 (MQ=255)
                    gCTCGCATTAAAAGTTATGCCAGCGGGGGGGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGc                                                                      <  2:1467960/93‑1 (MQ=255)
                     cTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCg                                                                     >  2:2328862/1‑93 (MQ=255)
                     cTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCg                                                                     >  2:5468214/1‑93 (MQ=255)
                      tCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCgg                                                                    >  1:6654261/1‑93 (MQ=255)
                       cGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCggg                                                                   >  1:5938254/1‑93 (MQ=255)
                           ttAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTg                                                               >  1:5108150/1‑93 (MQ=255)
                           ttAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTg                                                               >  1:1485332/1‑93 (MQ=255)
                            tAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGc                                                              <  1:8747852/93‑1 (MQ=255)
                            tAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGc                                                              >  2:4299657/1‑93 (MQ=255)
                            tAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGc                                                              <  1:8105153/93‑1 (MQ=255)
                              aaaGTTATGCCAGCGGTGGCTTGCCCCCCGCATTGATGGGTATGGGGCCATTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCaa                                                            <  2:7042120/93‑1 (MQ=255)
                              aaaGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCaa                                                            <  1:4087710/93‑1 (MQ=255)
                              aaaGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCaa                                                            >  2:3169417/1‑93 (MQ=255)
                               aaGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAAc                                                           <  2:503474/93‑1 (MQ=255)
                                aGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACt                                                          <  2:4087462/93‑1 (MQ=255)
                                 gTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTg                                                         <  2:9402407/93‑1 (MQ=255)
                                 gTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGC‑A‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTgg                                                        <  1:624507/93‑1 (MQ=255)
                                  ttATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTgg                                                        <  1:7900036/93‑1 (MQ=255)
                                       ccAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGat                                                   >  1:1330783/1‑93 (MQ=255)
                                         aGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGatat                                                 <  1:9268618/93‑1 (MQ=255)
                                         aGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGCACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGCCGGatat                                                 >  1:1708843/1‑93 (MQ=255)
                                          gCGGTGGCGGGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATAtt                                                <  2:2786720/93‑1 (MQ=255)
                                           cGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTg                                               >  2:7617190/1‑93 (MQ=255)
                                           cGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGAACCTGCCACGCAA‑AAA‑AGCGTTACAACTTGCTGGGCTGCAACTGGCGGgtaata                                               >  2:5306194/1‑90 (MQ=255)
                                             gTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGAt                                             <  1:4333151/93‑1 (MQ=255)
                                               ggCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGAtct                                           <  1:5489604/93‑1 (MQ=255)
                                                 cGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCa                                         <  2:8952544/93‑1 (MQ=255)
                                                  gTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCAt                                        <  1:7596674/93‑1 (MQ=255)
                                                  gTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCAt                                        <  1:8968546/93‑1 (MQ=255)
                                                  gTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCAt                                        <  1:5429519/93‑1 (MQ=255)
                                                   tGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCAtt                                       <  2:8625690/93‑1 (MQ=255)
                                                     ccccccGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGa                                     >  2:6260244/1‑93 (MQ=255)
                                                     ccccccGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGa                                     >  1:1212749/1‑93 (MQ=255)
                                                      cccccGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAg                                    >  1:3700874/1‑93 (MQ=255)
                                                       ccccGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAgg                                   <  2:2258827/93‑1 (MQ=255)
                                                        cccGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGc                                  <  2:3912689/93‑1 (MQ=255)
                                                        cccGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGc                                  >  2:2285071/1‑93 (MQ=255)
                                                        cccGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGc                                  >  1:3595463/1‑93 (MQ=255)
                                                         ccGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCt                                 >  1:3218303/1‑93 (MQ=255)
                                                          cGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTa                                <  1:9813257/93‑1 (MQ=255)
                                                           gCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTaa                               <  2:4705599/93‑1 (MQ=255)
                                                            cATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGGAACTGGCGGATATTGATCTCATTGAGGCTAAt                              >  1:2538545/1‑93 (MQ=255)
                                                            cATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAAt                              <  1:2979218/93‑1 (MQ=255)
                                                            cATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAAt                              <  1:7732163/93‑1 (MQ=255)
                                                                gATGGGTATGGGGCCAGTACCTGCCACGC‑A‑AAATAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAg                          <  1:9669362/93‑1 (MQ=255)
                                                                gATGGGTATGGGGCCAGTACCTGCCACGC‑A‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGc                         <  2:4627833/93‑1 (MQ=255)
                                                                 aTGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGc                         >  1:1190197/1‑93 (MQ=255)
                                                                  tGGTTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCa                        <  1:4794473/93‑1 (MQ=255)
                                                                  tGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCa                        <  1:2658324/93‑1 (MQ=255)
                                                                  tGGGTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCa                        >  2:8137202/1‑93 (MQ=255)
                                                                    ggTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCAtt                      <  1:5175612/93‑1 (MQ=255)
                                                                     gTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCAttt                     <  2:638405/93‑1 (MQ=255)
                                                                     gTATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCAttt                     <  2:5403221/93‑1 (MQ=255)
                                                                      tATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTtg                    >  2:2411654/1‑93 (MQ=255)
                                                                      tATGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTtg                    <  1:7080810/93‑1 (MQ=255)
                                                                       aTGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTtgc                   <  2:4141058/93‑1 (MQ=255)
                                                                        tGGGGCCAGTACCTGCCACGCAA‑AAA‑AGCGTCACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTtgct                  >  1:7216905/1‑93 (MQ=255)
                                                                            gCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGcac              <  2:1657283/93‑1 (MQ=255)
                                                                            gCCAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGcac              <  2:7803362/93‑1 (MQ=255)
                                                                             ccAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGcaca             <  1:6827545/93‑1 (MQ=255)
                                                                             ccAGTACCTGCCACGC‑A‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAg            <  1:281241/93‑1 (MQ=255)
                                                                              cAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAg            <  1:2395011/93‑1 (MQ=255)
                                                                              cAGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAg            >  2:7882941/1‑93 (MQ=255)
                                                                               aGTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGt           >  2:4919058/1‑93 (MQ=255)
                                                                                gTACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGtt          >  1:9720228/1‑93 (MQ=255)
                                                                                 tACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTc         >  2:2814405/1‑93 (MQ=255)
                                                                                 tACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTc         >  2:7275010/1‑93 (MQ=255)
                                                                                 tACCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTc         >  2:3005415/1‑93 (MQ=255)
                                                                                 tACCTGCCACGC‑A‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTcc        >  2:9263914/1‑93 (MQ=255)
                                                                                  aCCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTcc        <  1:1048350/93‑1 (MQ=255)
                                                                                  aCCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTcc        >  1:4434007/1‑93 (MQ=255)
                                                                                  aCCTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTcc        <  1:9958942/93‑1 (MQ=255)
                                                                                   ccTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCt       <  2:1271596/93‑1 (MQ=255)
                                                                                   ccTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCt       <  2:2586760/93‑1 (MQ=255)
                                                                                    cTGCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCAAAGTTCCtt      >  2:4774942/1‑93 (MQ=255)
                                                                                    cTGCCACGC‑A‑AAA‑AGCGTTACAACTGGAGGGGCTGCAACTGGGGGATATTGATCTCATTGAGGCAAATGAAGGCTTTGCCGCACGGGTCCTTg     >  2:7421061/1‑93 (MQ=255)
                                                                                     tGCCACGC‑AGAAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTg     <  1:4075330/93‑1 (MQ=255)
                                                                                     tGCCACGC‑A‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGc    >  1:4954407/1‑93 (MQ=255)
                                                                                      gCCACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGc    >  2:5825135/1‑93 (MQ=255)
                                                                                        cACGCAA‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCg  >  1:7426794/1‑93 (MQ=255)
                                                                                             |                                                                                         
CAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGC‑A‑AAA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCG  >  NC_000913/2324928‑2325107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGC‑A‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAAAAAC  >  NC_000913/2324932‑2325118
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AGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGC‑A‑A‑AA‑A                                                                                               >  SRR4302540.5128952/1‑94 (MQ=60)
 GGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGC‑A‑A‑AA‑AA                                                                                              <  SRR4302540.6366112/94‑1 (MQ=60)
  GCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGC‑A‑A‑AA‑Aag                                                                                             >  SRR4302540.208189/1‑92 (MQ=60)
    CTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCG                                                                                            <  SRR4302540.5779737/94‑1 (MQ=60)
    CTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCG                                                                                            >  SRR4302540.4637093/1‑94 (MQ=60)
    CTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCG                                                                                            >  SRR4302540.9198240/1‑94 (MQ=60)
    CTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGCATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCG                                                                                            >  SRR4302540.5471008/1‑94 (MQ=60)
       ACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTA                                                                                         <  SRR4302540.967317/94‑1 (MQ=60)
        CCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTAC                                                                                        <  SRR4302540.612169/94‑1 (MQ=60)
          CCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAA                                                                                      >  SRR4302540.7077419/1‑94 (MQ=60)
          CCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAA                                                                                      <  SRR4302540.8674729/94‑1 (MQ=60)
           CCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAAC                                                                                     >  SRR4302540.2089222/1‑94 (MQ=60)
            CCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGC‑A‑A‑AA‑AGCGTTACAACTG                                                                                   <  SRR4302540.8267577/94‑1 (MQ=60)
             CTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTG                                                                                   <  SRR4302540.6246753/94‑1 (MQ=60)
             CTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTG                                                                                   >  SRR4302540.6731795/1‑94 (MQ=60)
              TGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGG                                                                                  <  SRR4302540.868218/94‑1 (MQ=60)
              CGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGG                                                                                  <  SRR4302540.8456125/94‑1 (MQ=60)
               GGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGC                                                                                 <  SRR4302540.7433781/94‑1 (MQ=60)
               GGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGC                                                                                 >  SRR4302540.7825754/1‑94 (MQ=60)
                 CTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGG                                                                               >  SRR4302540.6727860/1‑94 (MQ=60)
                 CTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGG                                                                               >  SRR4302540.4491970/1‑94 (MQ=60)
                  TCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGG                                                                              >  SRR4302540.4893343/1‑94 (MQ=60)
                  TCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGG                                                                              >  SRR4302540.6003897/1‑94 (MQ=60)
                  TCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCCCATTGATGGGTATGGGGCCAGTACCGGCCACGCAA‑A‑AA‑AGCGTTAACACTGGCGGG                                                                              >  SRR4302540.6003898/1‑94 (MQ=60)
                     CATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCT                                                                           <  SRR4302540.5925881/94‑1 (MQ=60)
                     CATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCT                                                                           >  SRR4302540.2614642/1‑94 (MQ=60)
                     CATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCT                                                                           <  SRR4302540.1192037/94‑1 (MQ=60)
                      ATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTG                                                                          >  SRR4302540.5163443/1‑94 (MQ=60)
                      ATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTG                                                                          >  SRR4302540.1479530/1‑94 (MQ=60)
                      ATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTG                                                                          >  SRR4302540.1501641/1‑94 (MQ=60)
                       TTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGC‑A‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCA                                                                        <  SRR4302540.7441598/94‑1 (MQ=60)
                        TAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCA                                                                        <  SRR4302540.8849366/94‑1 (MQ=60)
                        TAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCA                                                                        >  SRR4302540.5296401/1‑94 (MQ=60)
                        TAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCA                                                                        <  SRR4302540.8199226/94‑1 (MQ=60)
                         AAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAA                                                                       >  SRR4302540.8457614/1‑94 (MQ=60)
                          AAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAAC                                                                      <  SRR4302540.4131793/94‑1 (MQ=60)
                          AAAGTTAGGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAAC                                                                      <  SRR4302540.564105/94‑1 (MQ=60)
                           AAGTTATGCCAGCGGTGGCGTGCCCCCCGCATGGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACT                                                                     <  SRR4302540.4760524/94‑1 (MQ=60)
                             GTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGC‑A‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGC                                                                  <  SRR4302540.631154/94‑1 (MQ=60)
                              TTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGC                                                                  <  SRR4302540.5827610/94‑1 (MQ=60)
                              TTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGC                                                                  <  SRR4302540.7992184/94‑1 (MQ=60)
                                ATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGG                                                                <  SRR4302540.9512049/94‑1 (MQ=60)
                                  GCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGAT                                                              >  SRR4302540.1344692/1‑94 (MQ=60)
                                    CAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATAT                                                            <  SRR4302540.9876449/94‑1 (MQ=60)
                                    CAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGCACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGCCGGATAT                                                            >  SRR4302540.1727306/1‑94 (MQ=60)
                                     AGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATT                                                           <  SRR4302540.9379462/94‑1 (MQ=60)
                                     AGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATT                                                           <  SRR4302540.8324118/94‑1 (MQ=60)
                                        GGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGC‑A‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATC                                                       >  SRR4302540.4152270/1‑94 (MQ=60)
                                         GTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATC                                                       >  SRR4302540.1289365/1‑94 (MQ=60)
                                         GTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATC                                                       <  SRR4302540.4380320/94‑1 (MQ=60)
                                           GGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTC                                                     <  SRR4302540.5550040/94‑1 (MQ=60)
                                           GGCGCGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTC                                                     >  SRR4302540.4453167/1‑94 (MQ=60)
                                              GTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATT                                                  <  SRR4302540.7682019/94‑1 (MQ=60)
                                              GTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATT                                                  <  SRR4302540.9074927/94‑1 (MQ=60)
                                              GTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATT                                                  <  SRR4302540.5489236/94‑1 (MQ=60)
                                                GCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGA                                                >  SRR4302540.654047/1‑94 (MQ=60)
                                                GCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGA                                                >  SRR4302540.3262121/1‑94 (MQ=60)
                                                GCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGA                                                >  SRR4302540.1225181/1‑94 (MQ=60)
                                                 CCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAG                                               >  SRR4302540.3741273/1‑94 (MQ=60)
                                                 CCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAG                                               >  SRR4302540.3739722/1‑94 (MQ=60)
                                                 CCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAG                                               <  SRR4302540.3164589/94‑1 (MQ=60)
                                                  CCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGG                                              >  SRR4302540.5224350/1‑94 (MQ=60)
                                                   CCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGC                                             >  SRR4302540.3634750/1‑94 (MQ=60)
                                                   CCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGC                                             >  SRR4302540.2913863/1‑94 (MQ=60)
                                                    CCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCT                                            >  SRR4302540.3253261/1‑94 (MQ=60)
                                                      CGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAA                                          <  SRR4302540.9928100/94‑1 (MQ=60)
                                                       GCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGGAACTGGCGGATATTGATCTCATTGAGGCTAAT                                         >  SRR4302540.2565919/1‑94 (MQ=60)
                                                       GCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAAT                                         >  SRR4302540.1552272/1‑94 (MQ=60)
                                                       GCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAAT                                         >  SRR4302540.1217951/1‑94 (MQ=60)
                                                       GCATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGAAACTGGCGGATATTGATCTCATTGATGATAAT                                         >  SRR4302540.2210391/1‑94 (MQ=60)
                                                        CATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATG                                        <  SRR4302540.7822425/94‑1 (MQ=60)
                                                        CATTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATG                                        <  SRR4302540.3012127/94‑1 (MQ=60)
                                                          TTGATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAA                                      >  SRR4302540.3757749/1‑94 (MQ=60)
                                                            GATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGC                                    >  SRR4302540.4415995/1‑94 (MQ=60)
                                                            GATGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGC                                    >  SRR4302540.1202466/1‑94 (MQ=60)
                                                            GATGGGTATGGGGCCAGTACCTGCCACGC‑A‑A‑AATAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGC                                    <  SRR4302540.9783164/94‑1 (MQ=60)
                                                              TGGTTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCAT                                  <  SRR4302540.4845762/94‑1 (MQ=60)
                                                              TGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCAT                                  <  SRR4302540.274832/94‑1 (MQ=60)
                                                              TGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCAT                                  >  SRR4302540.4130723/1‑94 (MQ=60)
                                                              TGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCAT                                  <  SRR4302540.2687170/94‑1 (MQ=60)
                                                              TGGGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCAT                                  <  SRR4302540.9228372/94‑1 (MQ=60)
                                                               GGGTATGGGGCCAGTACCTGCCACGC‑A‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTT                                <  SRR4302540.7401347/94‑1 (MQ=60)
                                                                GGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTT                                <  SRR4302540.5231941/94‑1 (MQ=60)
                                                                GGTATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTT                                <  SRR4302540.631876/94‑1 (MQ=60)
                                                                  TATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGC                              <  SRR4302540.7159621/94‑1 (MQ=60)
                                                                   ATGGGGCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTCACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCT                             >  SRR4302540.7297346/1‑94 (MQ=60)
                                                                        GCCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACA                        >  SRR4302540.7208004/1‑94 (MQ=60)
                                                                         CCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAG                       <  SRR4302540.6903145/94‑1 (MQ=60)
                                                                         CCAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAG                       <  SRR4302540.9707920/94‑1 (MQ=60)
                                                                         CCAGTACCTGCCACGC‑A‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGT                      <  SRR4302540.284001/94‑1 (MQ=60)
                                                                         CCAGTACCTGCCACGC‑A‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGT                      <  SRR4302540.3894149/94‑1 (MQ=60)
                                                                          CAGTTCCTGCCACGG‑A‑ACAA‑AGCGTTACCACAGGCGGGGCTGCAACTGTCTGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGT                      <  SRR4302540.2420818/94‑1 (MQ=60)
                                                                          CAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGT                      <  SRR4302540.2420817/94‑1 (MQ=60)
                                                                          CAGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGT                      >  SRR4302540.5542073/1‑94 (MQ=60)
                                                                           AGTACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTT                     >  SRR4302540.9834421/1‑94 (MQ=60)
                                                                             TACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCC                   >  SRR4302540.4482049/1‑94 (MQ=60)
                                                                              ACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCT                  <  SRR4302540.1059010/94‑1 (MQ=60)
                                                                              ACCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCT                  <  SRR4302540.10075166/94‑1 (MQ=60)
                                                                               CCTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTT                 <  SRR4302540.10069976/94‑1 (MQ=60)
                                                                                CTGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTG                <  SRR4302540.7725413/94‑1 (MQ=60)
                                                                                CTGCCACGC‑A‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGC               >  SRR4302540.5007939/1‑94 (MQ=60)
                                                                                 TGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGTCTGCAACTGGCGGATATTGATTTCATTTGGGCTAATGAAGCATTTGCTGCACATTTCCTTGC               >  SRR4302540.123039/1‑94 (MQ=60)
                                                                                 TGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGC               >  SRR4302540.8969414/1‑94 (MQ=60)
                                                                                 TGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGC               >  SRR4302540.5294160/1‑94 (MQ=60)
                                                                                 TGCCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGC               <  SRR4302540.7602738/94‑1 (MQ=60)
                                                                                 TGCCACGC‑AGA‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGC               <  SRR4302540.4119257/94‑1 (MQ=60)
                                                                                   CCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGTCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCG             <  SRR4302540.9824938/94‑1 (MQ=60)
                                                                                   CCACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCG             >  SRR4302540.7509530/1‑94 (MQ=60)
                                                                                    CACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGT            <  SRR4302540.8125113/94‑1 (MQ=60)
                                                                                    CACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGT            >  SRR4302540.8726348/1‑94 (MQ=60)
                                                                                     ACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTT           <  SRR4302540.196250/94‑1 (MQ=60)
                                                                                     ACGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTT           >  SRR4302540.6556992/1‑94 (MQ=60)
                                                                                      CGCAA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTG          <  SRR4302540.2815441/94‑1 (MQ=60)
                                                                                       gcaA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGG         <  SRR4302540.1004781/91‑1 (MQ=60)
                                                                                       gcaA‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGG         <  SRR4302540.1201506/91‑1 (MQ=60)
                                                                                        A‑A‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGA       >  SRR4302540.1458729/1‑94 (MQ=60)
                                                                                        A‑A‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGA       >  SRR4302540.8430544/1‑94 (MQ=60)
                                                                                        A‑A‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGA       >  SRR4302540.7450156/1‑94 (MQ=60)
                                                                                        A‑A‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGA       >  SRR4302540.8430543/1‑94 (MQ=60)
                                                                                        A‑A‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGA       >  SRR4302540.6361100/1‑94 (MQ=60)
                                                                                          A‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAA      >  SRR4302540.5091519/1‑94 (MQ=60)
                                                                                          A‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAA      <  SRR4302540.773387/94‑1 (MQ=60)
                                                                                            A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAAA     >  SRR4302540.3909560/1‑94 (MQ=60)
                                                                                            A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAAA     <  SRR4302540.7342768/94‑1 (MQ=60)
                                                                                            A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAAA     <  SRR4302540.5877849/94‑1 (MQ=60)
                                                                                            A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAAA     <  SRR4302540.7342769/94‑1 (MQ=60)
                                                                                              AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAAAA    <  SRR4302540.1418144/94‑1 (MQ=60)
                                                                                               A‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAAAAA   <  SRR4302540.9331020/94‑1 (MQ=60)
                                                                                                 AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAAAAAC  <  SRR4302540.3162204/94‑1 (MQ=60)
                                                                                                 |                                                                                             
AGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGC‑A‑A‑AA‑AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAAAAAC  >  NC_000913/2324932‑2325118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑