Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,325,025 (A)5→6 coding (897/1185 nt) atoB → acetyl‑CoA acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,325,0201.A100.0% 724.5 / NA 191Q298K (CAA→AAA) atoBacetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (93/98);  total (93/98)

Only 100 of 209 total aligned reads displayed.

CAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑C‑AAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCG  >  NC_000913/2324928‑2325107
                                                                                              |                                                                                       
cagcagGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑c                                                                                          >  1:4022843/1‑93 (MQ=255)
cagcagGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑c                                                                                          <  2:1187162/93‑1 (MQ=255)
  gcagGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑C‑aa                                                                                       >  2:1248497/1‑93 (MQ=255)
     ggCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑C‑aaaaa                                                                                    >  1:5940671/1‑93 (MQ=255)
         ttACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑C‑aaaaaagcg                                                                                <  1:1458009/93‑5 (MQ=255)
         ttACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑C‑aaaaaagcg                                                                                <  1:294938/93‑5 (MQ=255)
           aCCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGtt                                                                               >  1:1231130/1‑93 (MQ=255)
            ccccccTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTa                                                                              <  2:5681051/93‑1 (MQ=255)
             cccccTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTAc                                                                             >  1:417958/1‑93 (MQ=255)
             cccccTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTAc                                                                             >  2:485478/1‑93 (MQ=255)
             cccccTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTAc                                                                             <  2:782013/93‑1 (MQ=255)
             cccccTGGCTCGCATTAAAAGTTATGCCAGCGGGGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTAc                                                                             >  1:4986766/1‑93 (MQ=255)
              ccccTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACa                                                                            >  1:3229137/1‑93 (MQ=255)
              ccccTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACa                                                                            >  1:6124771/1‑93 (MQ=255)
              ccccTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACa                                                                            <  1:3775754/93‑1 (MQ=255)
               cccTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACaa                                                                           >  2:2387930/1‑93 (MQ=255)
               cccTGGCTCGCATGAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGGATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACaa                                                                           <  1:4275764/93‑1 (MQ=255)
                ccTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAAc                                                                          <  2:1743108/93‑1 (MQ=255)
                 cTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACt                                                                         <  1:4798859/93‑1 (MQ=255)
                 cTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACt                                                                         >  2:5873846/1‑93 (MQ=255)
                   ggCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTgg                                                                       <  1:988441/93‑1 (MQ=255)
                   ggCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTgg                                                                       >  2:529064/1‑93 (MQ=255)
                   ggCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTgg                                                                       >  2:212835/1‑93 (MQ=255)
                     cTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCg                                                                     <  2:6150724/93‑1 (MQ=255)
                        gCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCgggg                                                                  <  2:1315502/93‑1 (MQ=255)
                          aTTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCt                                                                <  2:4014837/93‑1 (MQ=255)
                           ttAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTg                                                               >  1:679112/1‑93 (MQ=255)
                             aaaaGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCa                                                             <  2:6029593/93‑1 (MQ=255)
                             aaaaGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCa                                                             >  1:6221987/1‑93 (MQ=255)
                               aaGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAAc                                                           <  1:1906201/93‑1 (MQ=255)
                               aaGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAAc                                                           <  2:1696602/93‑1 (MQ=255)
                                aGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGTCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACt                                                          <  1:6043509/93‑1 (MQ=255)
                                aGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACt                                                          <  2:3730550/93‑1 (MQ=255)
                                  ttATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTgg                                                        <  2:5476519/93‑1 (MQ=255)
                                  ttATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTgc                                                        >  2:5188564/1‑92 (MQ=255)
                                            ggTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGa                                              <  1:5777051/93‑1 (MQ=255)
                                             gTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGAt                                             >  1:948278/1‑93 (MQ=255)
                                              tGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGAtc                                            >  2:5591694/1‑93 (MQ=255)
                                               ggCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGAtct                                           >  2:588367/1‑93 (MQ=255)
                                               ggCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGAtct                                           <  1:531257/93‑1 (MQ=255)
                                               ggCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGAGCTGCAACTTGCGGATAATGAtct                                           >  2:1806890/1‑93 (MQ=255)
                                                gCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGAtctc                                          <  1:1042181/93‑1 (MQ=255)
                                                 cGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCa                                         <  2:3288882/93‑1 (MQ=255)
                                                  gTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCAt                                        <  1:5274449/93‑1 (MQ=255)
                                                   tGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCAtt                                       <  1:6242814/93‑1 (MQ=255)
                                                    gCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTg                                      >  1:3468305/1‑93 (MQ=255)
                                                    gCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTg                                      <  2:4139024/93‑1 (MQ=255)
                                                      cccccGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAg                                    >  1:5511988/1‑93 (MQ=255)
                                                       ccccGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAgg                                   <  1:5681914/93‑1 (MQ=255)
                                                       ccccGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAgg                                   >  2:5001147/1‑93 (MQ=255)
                                                        cccGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGc                                  <  1:5994213/93‑1 (MQ=255)
                                                        cccGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGc                                  <  1:5569931/93‑1 (MQ=255)
                                                         ccGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCt                                 >  2:4920162/1‑93 (MQ=255)
                                                         ccGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCt                                 >  2:468371/1‑93 (MQ=255)
                                                          cGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTa                                <  1:1321473/93‑1 (MQ=255)
                                                           gCATTGATGGGTATGGGGCTAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAGCTGGCAGATATTGATCTCATTGAGGCTaa                               <  1:3276333/93‑1 (MQ=255)
                                                           gCATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTaa                               <  2:1460695/93‑1 (MQ=255)
                                                            cATTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAAt                              <  2:3677114/93‑1 (MQ=255)
                                                             aTTGATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATg                             >  1:4099507/1‑93 (MQ=255)
                                                                gATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAg                          <  1:1764183/93‑1 (MQ=255)
                                                                gATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAg                          >  1:3504884/1‑93 (MQ=255)
                                                                gATGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAg                          >  2:4988375/1‑93 (MQ=255)
                                                                 aTGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGc                         <  1:748452/93‑1 (MQ=255)
                                                                 aTGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGc                         <  1:4476541/93‑1 (MQ=255)
                                                                 aTGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGc                         >  1:3820285/1‑93 (MQ=255)
                                                                  tGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCa                        <  1:453409/93‑1 (MQ=255)
                                                                  tGGGTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCa                        >  1:4667130/1‑93 (MQ=255)
                                                                    ggTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCAtt                      <  2:4352556/93‑1 (MQ=255)
                                                                    ggTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCAtt                      >  1:6257648/1‑93 (MQ=255)
                                                                     gTATGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCAttt                     <  2:3570141/93‑1 (MQ=255)
                                                                        tGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGGTAATGAAGCATTtgct                  >  1:2630088/1‑93 (MQ=255)
                                                                        tGGGGCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTtgct                  <  1:5877729/93‑1 (MQ=255)
                                                                         ggggCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTtgctg                 <  1:214297/93‑1 (MQ=255)
                                                                          gggCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTtgctgc                >  1:5178178/1‑93 (MQ=255)
                                                                          gggCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTtgctgc                <  2:3393057/93‑1 (MQ=255)
                                                                            gCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGcac              <  2:5175147/93‑1 (MQ=255)
                                                                            gCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGcac              >  2:4744626/1‑93 (MQ=255)
                                                                            gCCAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGcac              <  1:3329212/93‑1 (MQ=255)
                                                                             ccAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATATCATTGAGGCTAATGAAGCATTTGCTGcaca             <  2:3229137/93‑1 (MQ=255)
                                                                              cAGTACCTGCCACGAA‑AAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTCATCTCATTGAGGCTAATGAAGCATTTGCTGCACAg            <  2:876335/93‑1 (MQ=255)
                                                                              cAGTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAg            <  2:3165380/93‑1 (MQ=255)
                                                                                gTACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGtt          >  2:3532678/1‑93 (MQ=255)
                                                                                 tACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTc         >  2:1199086/1‑93 (MQ=255)
                                                                                 tACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTc         <  2:1042084/93‑1 (MQ=255)
                                                                                 tACCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTc         <  2:2925133/93‑1 (MQ=255)
                                                                                  aCCTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTcc        <  1:4140498/93‑1 (MQ=255)
                                                                                   ccTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCt       <  1:907375/93‑1 (MQ=255)
                                                                                    cggCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCtt      >  1:6090676/3‑93 (MQ=255)
                                                                                    cTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCtt      >  2:629846/1‑93 (MQ=255)
                                                                                    cTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCtt      >  1:4454983/1‑93 (MQ=255)
                                                                                    cTGCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAAGCTAATGAAGCATTTGCTGCACAGTTCCtt      >  1:2461025/1‑93 (MQ=255)
                                                                                     tGCCACG‑GAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTg     >  2:5763981/1‑93 (MQ=255)
                                                                                      gCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGc    >  1:3001968/1‑93 (MQ=255)
                                                                                      gCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGc    >  1:2264446/1‑93 (MQ=255)
                                                                                      gCCACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGc    >  1:2220352/1‑93 (MQ=255)
                                                                                       ccACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGcc   <  2:2454443/93‑1 (MQ=255)
                                                                                       ccACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGcc   <  1:1332201/93‑1 (MQ=255)
                                                                                        cACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCg  >  2:2000056/1‑93 (MQ=255)
                                                                                        cACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCg  >  1:3134287/1‑93 (MQ=255)
                                                                                        cACG‑CAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCg  >  1:3689833/1‑93 (MQ=255)
                                                                                              |                                                                                       
CAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACG‑C‑AAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCG  >  NC_000913/2324928‑2325107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGC‑AAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAAAAAC  >  NC_000913/2324932‑2325118
                                                                                              |                                                                                             
AGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGC‑AAAAA                                                                                               >  SRR4302533.5976595/1‑94 (MQ=60)
   CCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGC‑AAAAAagc                                                                                            >  SRR4302533.6107311/1‑91 (MQ=60)
     TTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGT                                                                                           <  SRR4302533.1466800/94‑1 (MQ=60)
     TTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGT                                                                                           <  SRR4302533.296739/94‑1 (MQ=60)
      TACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTT                                                                                          >  SRR4302533.1238500/1‑94 (MQ=60)
        CCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTAC                                                                                        >  SRR4302533.420464/1‑94 (MQ=60)
        CCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGGGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTAC                                                                                        >  SRR4302533.5017003/1‑94 (MQ=60)
         CCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACA                                                                                       >  SRR4302533.6161689/1‑94 (MQ=60)
         CCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACA                                                                                       <  SRR4302533.1721071/94‑1 (MQ=60)
         CCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACA                                                                                       >  SRR4302533.3249047/1‑94 (MQ=60)
          CCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAA                                                                                      <  SRR4302533.3798898/94‑1 (MQ=60)
           ccggtgcttgcattaaaagttagtgcatcggcGGCGTTCCCCCCGCATTGAGTGTTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAAC                                                                                     <  SRR4302533.4989600/62‑1 (MQ=33)
           CCCTGGCTCGCATGAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGGATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAAC                                                                                     <  SRR4302533.4301483/94‑1 (MQ=60)
             CTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTG                                                                                   <  SRR4302533.4827928/94‑1 (MQ=60)
              TGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGG                                                                                  <  SRR4302533.1315015/94‑1 (MQ=60)
              TGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGG                                                                                  >  SRR4302533.5464318/1‑94 (MQ=60)
              TGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGG                                                                                  <  SRR4302533.6267333/94‑1 (MQ=60)
               GGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGC                                                                                 <  SRR4302533.994225/94‑1 (MQ=60)
               GGCTCGCATTAAAAGTTATGCCAGCAGTGGCGTGCCCCCCGCATTGATGGGTATGGGTCCAGTCCCTGCCACGCAAAAAAGCGTTACAACTGGC                                                                                 <  SRR4302533.6121361/94‑1 (MQ=60)
                GCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGCCCAGTCCCTGCCACGCAAAAAAGCGTTACAACTGGCG                                                                                <  SRR4302533.5392293/94‑1 (MQ=60)
                 CTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGG                                                                               >  SRR4302533.4748392/1‑94 (MQ=60)
                 CTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGG                                                                               >  SRR4302533.360874/1‑94 (MQ=60)
                 CTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGG                                                                               >  SRR4302533.5997790/1‑94 (MQ=60)
                  TCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGG                                                                              >  SRR4302533.2268185/1‑94 (MQ=60)
                  TCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGG                                                                              >  SRR4302533.3031390/1‑94 (MQ=60)
                    GCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGC                                                                            >  SRR4302533.3273217/1‑94 (MQ=60)
                    GCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGC                                                                            >  SRR4302533.454973/1‑94 (MQ=60)
                     CATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCT                                                                           <  SRR4302533.6052843/94‑1 (MQ=60)
                      ATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTG                                                                          >  SRR4302533.683126/1‑94 (MQ=60)
                        TAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCA                                                                        >  SRR4302533.6259279/1‑94 (MQ=60)
                         AAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAA                                                                       >  SRR4302533.890785/1‑94 (MQ=60)
                         AAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAA                                                                       >  SRR4302533.6698/1‑94 (MQ=60)
                          AAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAAC                                                                      <  SRR4302533.5701969/94‑1 (MQ=60)
                           AAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACT                                                                     <  SRR4302533.1917828/94‑1 (MQ=60)
                            AGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGTCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTG                                                                    <  SRR4302533.6080124/94‑1 (MQ=60)
                             GTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGG                                                                   <  SRR4302533.4601349/94‑1 (MQ=60)
                               TATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCG                                                                 >  SRR4302533.2698937/1‑94 (MQ=60)
                                  GCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGAT                                                              <  SRR4302533.221297/94‑1 (MQ=60)
                                    CAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATAT                                                            <  SRR4302533.934704/94‑1 (MQ=60)
                                    CAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATAT                                                            <  SRR4302533.5918549/94‑1 (MQ=60)
                                       CGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGA                                                         <  SRR4302533.3303699/94‑1 (MQ=60)
                                       CGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGA                                                         >  SRR4302533.985726/1‑94 (MQ=60)
                                        GGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGAT                                                        <  SRR4302533.5811943/94‑1 (MQ=60)
                                        GGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGAT                                                        >  SRR4302533.2369748/1‑94 (MQ=60)
                                        GGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGAT                                                        >  SRR4302533.953850/1‑94 (MQ=60)
                                          TGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCT                                                      <  SRR4302533.6157812/94‑1 (MQ=60)
                                          TGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCT                                                      <  SRR4302533.1330779/94‑1 (MQ=60)
                                           GGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTC                                                     <  SRR4302533.534417/94‑1 (MQ=60)
                                            GCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCA                                                    <  SRR4302533.5101114/94‑1 (MQ=60)
                                            GCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCA                                                    <  SRR4302533.1048309/94‑1 (MQ=60)
                                              GTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATT                                                  <  SRR4302533.5036454/94‑1 (MQ=60)
                                              GTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATT                                                  <  SRR4302533.5306074/94‑1 (MQ=60)
                                               TGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTG                                                 <  SRR4302533.6280185/94‑1 (MQ=60)
                                               TGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTG                                                 >  SRR4302533.3489715/1‑94 (MQ=60)
                                                 CCCCCCGCATTGATGGGTATGGGTCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAG                                               <  SRR4302533.5310042/94‑1 (MQ=60)
                                                 CCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAG                                               <  SRR4302533.3847279/94‑1 (MQ=60)
                                                 CCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAG                                               >  SRR4302533.5545263/1‑94 (MQ=60)
                                                 CCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTCACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAG                                               <  SRR4302533.3404082/94‑1 (MQ=60)
                                                  CCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGG                                              >  SRR4302533.4256779/1‑94 (MQ=60)
                                                   CCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGC                                             <  SRR4302533.5716233/94‑1 (MQ=60)
                                                    CCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGTCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCT                                            <  SRR4302533.412754/94‑1 (MQ=60)
                                                    CCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCT                                            <  SRR4302533.6030517/94‑1 (MQ=60)
                                                    CCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCT                                            <  SRR4302533.5603597/94‑1 (MQ=60)
                                                     CCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTA                                           >  SRR4302533.4195532/1‑94 (MQ=60)
                                                     CCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTA                                           >  SRR4302533.6206068/1‑94 (MQ=60)
                                                      CGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAA                                          <  SRR4302533.1329404/94‑1 (MQ=60)
                                                       GCATTGATGGGTATGGGGCTAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAGCTGGCAGATATTGATCTCATTGAGGCTAAT                                         <  SRR4302533.3296567/94‑1 (MQ=60)
                                                        CATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATG                                        >  SRR4302533.4124457/1‑94 (MQ=60)
                                                        CATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATG                                        >  SRR4302533.2996755/1‑94 (MQ=60)
                                                         ATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGA                                       >  SRR4302533.2776618/1‑94 (MQ=60)
                                                          TTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAA                                      <  SRR4302533.7166/94‑1 (MQ=60)
                                                           TGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAG                                     <  SRR4302533.5601195/94‑1 (MQ=60)
                                                           TGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAG                                     <  SRR4302533.1003472/94‑1 (MQ=60)
                                                           TGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAG                                     >  SRR4302533.3526491/1‑94 (MQ=60)
                                                            GATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGC                                    >  SRR4302533.3843645/1‑94 (MQ=60)
                                                            GATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGC                                    <  SRR4302533.1774925/94‑1 (MQ=60)
                                                             ATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCA                                   >  SRR4302533.4695406/1‑94 (MQ=60)
                                                             ATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCA                                   <  SRR4302533.752858/94‑1 (MQ=60)
                                                             ATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCA                                   <  SRR4302533.4503617/94‑1 (MQ=60)
                                                             ATGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCA                                   >  SRR4302533.1886649/1‑94 (MQ=60)
                                                              TGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCAT                                  <  SRR4302533.456120/94‑1 (MQ=60)
                                                              TGGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCAT                                  <  SRR4302533.6295027/94‑1 (MQ=60)
                                                               GGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATT                                 <  SRR4302533.5291078/94‑1 (MQ=60)
                                                               GGGTATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATT                                 >  SRR4302533.6295070/1‑94 (MQ=60)
                                                                  TATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGC                              >  SRR4302533.5741241/1‑94 (MQ=60)
                                                                   ATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGGTAATGAAGCATTTGCT                             >  SRR4302533.2646251/1‑94 (MQ=60)
                                                                   ATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCT                             <  SRR4302533.3245451/94‑1 (MQ=60)
                                                                   ATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCT                             <  SRR4302533.3813969/94‑1 (MQ=60)
                                                                   ATGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCT                             <  SRR4302533.2515774/94‑1 (MQ=60)
                                                                    TGGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTG                            <  SRR4302533.5913258/94‑1 (MQ=60)
                                                                     GGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGC                           <  SRR4302533.215646/94‑1 (MQ=60)
                                                                     GGGGCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGC                           >  SRR4302533.5209450/1‑94 (MQ=60)
                                                                        GCCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACA                        <  SRR4302533.3349788/94‑1 (MQ=60)
                                                                         CCAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAG                       >  SRR4302533.4985447/1‑94 (MQ=60)
                                                                          CAGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGT                      >  SRR4302533.3876963/1‑94 (MQ=60)
                                                                           AGTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTT                     >  SRR4302533.5847933/1‑94 (MQ=60)
                                                                            GTACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGCTATTGATCTCATTGAGGCTAATGAAGCATTTGTTGCACAGTTC                    >  SRR4302533.3779099/1‑94 (MQ=60)
                                                                              ACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGATGCTAATGAAGCATTTGCTGCACAGTTCCT                  >  SRR4302533.4124426/1‑94 (MQ=60)
                                                                              ACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCT                  >  SRR4302533.1096954/1‑94 (MQ=60)
                                                                              ACCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCT                  <  SRR4302533.4165609/94‑1 (MQ=60)
                                                                               CCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTT                 >  SRR4302533.444052/1‑94 (MQ=60)
                                                                               CCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTT                 <  SRR4302533.3502951/94‑1 (MQ=60)
                                                                               CCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTT                 <  SRR4302533.912696/94‑1 (MQ=60)
                                                                               CCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTT                 >  SRR4302533.4481920/1‑94 (MQ=60)
                                                                               CCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTT                 <  SRR4302533.4500011/94‑1 (MQ=60)
                                                                               CCTGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAAGCTAATGAAGCATTTGCTGCACAGTTCCTT                 >  SRR4302533.2476211/1‑94 (MQ=60)
                                                                               CCGGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTT                 >  SRR4302533.6127489/1‑94 (MQ=60)
                                                                                 TGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGC               >  SRR4302533.2234059/1‑94 (MQ=60)
                                                                                 TGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGC               >  SRR4302533.2278458/1‑94 (MQ=60)
                                                                                 TGCCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGC               >  SRR4302533.3020326/1‑94 (MQ=60)
                                                                                   CCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCG             <  SRR4302533.1340195/94‑1 (MQ=60)
                                                                                   CCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCG             >  SRR4302533.3153557/1‑94 (MQ=60)
                                                                                   CCACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCG             >  SRR4302533.3712502/1‑94 (MQ=60)
                                                                                    CACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGT            >  SRR4302533.2726797/1‑94 (MQ=60)
                                                                                     ACGCAAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTT           <  SRR4302533.3450970/94‑1 (MQ=60)
                                                                                       gcaAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGG         <  SRR4302533.3251245/91‑1 (MQ=60)
                                                                                       gcaAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGG         >  SRR4302533.439857/4‑94 (MQ=60)
                                                                                        caAAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGG        <  SRR4302533.3328415/92‑1 (MQ=60)
                                                                                        A‑AAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGA       <  SRR4302533.4643229/94‑1 (MQ=60)
                                                                                          AAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAA      >  SRR4302533.629569/1‑94 (MQ=60)
                                                                                          AAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAA      >  SRR4302533.1482946/1‑94 (MQ=60)
                                                                                          AAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAA      <  SRR4302533.1674797/94‑1 (MQ=60)
                                                                                          AAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAA      <  SRR4302533.173836/94‑1 (MQ=60)
                                                                                           AAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAAA     >  SRR4302533.1455594/1‑94 (MQ=60)
                                                                                             AAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAAAAA   <  SRR4302533.1757119/94‑1 (MQ=60)
                                                                                              AGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAAAAAC  >  SRR4302533.5605163/1‑94 (MQ=60)
                                                                                              |                                                                                             
AGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCCAGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGC‑AAAAAGCGTTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTTGCTGCACAGTTCCTTGCCGTTGGGAAAAAC  >  NC_000913/2324932‑2325118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑