Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,345,161	T→C	C759C (TGT→TGC)	nrdA →	ribonucleoside diphosphate reductase 1 alpha subunit, B1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,345,161	0	T	C	99.2%	856.7 / ‑3.6	264	C759C (TGT→TGC)	nrdA	ribonucleoside diphosphate reductase 1 alpha subunit, B1
Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (107/155); total (107/157)
Fisher's exact test for biased strand distribution p-value = 5.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 273 total aligned reads displayed.

GTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGTAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTC  >  NC_000913/2345071‑2345250

gTATTATCAGACCACCCGTGACGGCGCTGAAGACGCACAGGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCaa                                                                                         <  2:8954557/93‑1 (MQ=255)

gTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCaa                                                                                         >  2:7380923/1‑93 (MQ=255)

  attatCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGa                                                                                       <  2:865141/93‑1 (MQ=255)

  attatCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGa                                                                                       >  1:8617932/1‑93 (MQ=255)

   ttatCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGAt                                                                                      >  2:4606991/1‑93 (MQ=255)

   ttatCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGAt                                                                                      >  2:1662703/1‑93 (MQ=255)

    tatCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATc                                                                                     <  1:6353780/93‑1 (MQ=255)

      tCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGCTGGCTGCGAAAGCGGCGCATGCAAGATCTg                                                                                   >  2:9668370/1‑93 (MQ=255)

      tCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTg                                                                                   <  1:9274096/93‑1 (MQ=255)

       cAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGAGGCGCATGCAAGCTCTGa                                                                                  >  1:3510982/1‑93 (MQ=255)

        aGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGat                                                                                 >  1:2226454/1‑93 (MQ=255)

        aGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGat                                                                                 >  2:9680340/1‑93 (MQ=255)

            cacCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTg                                                                             >  2:1570415/1‑93 (MQ=255)

             acCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTga                                                                            >  2:2789996/1‑93 (MQ=255)

             acCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTga                                                                            <  1:6649219/93‑1 (MQ=255)

              cccGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTgag                                                                           >  2:9866171/1‑93 (MQ=255)

               ccGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTgaga                                                                          <  1:4532996/93‑1 (MQ=255)

               ccGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTgaga                                                                          <  2:6079858/93‑1 (MQ=255)

               ccGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTgaga                                                                          <  2:5835195/93‑1 (MQ=255)

               ccGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTgaga                                                                          <  1:4307307/93‑1 (MQ=255)

               ccGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTgaga                                                                          <  2:750902/93‑1 (MQ=255)

                cGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGAt                                                                         >  1:2107617/1‑93 (MQ=255)

                cGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGAt                                                                         >  2:3946406/1‑93 (MQ=255)

                 gTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATg                                                                        <  2:1286481/93‑1 (MQ=255)

                 gTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATg                                                                        >  2:503248/1‑93 (MQ=255)

                  tGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGc                                                                       <  1:4847152/93‑1 (MQ=255)

                  tGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGc                                                                       >  2:3338727/1‑93 (MQ=255)

                      ggCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGa                                                                   <  1:544511/93‑1 (MQ=255)

                      ggCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGa                                                                   <  1:3092328/93‑1 (MQ=255)

                       gcgcTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGAt                                                                  <  2:6716671/93‑1 (MQ=255)

                        ggcTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATg                                                                 <  2:2578255/92‑1 (MQ=255)

                         gcTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgc                                                                >  2:3638258/1‑93 (MQ=255)

                           tGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgcgg                                                              <  1:7349774/93‑1 (MQ=255)

                            gAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgcggc                                                             <  2:8208102/93‑1 (MQ=255)

                            gAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgcggc                                                             <  1:2824830/93‑1 (MQ=255)

                            gAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgcggc                                                             <  1:6816788/93‑1 (MQ=255)

                            gAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgcggc                                                             >  1:4788694/1‑93 (MQ=255)

                             aaGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGTAAGATCTGATATTGAGATGCCGGATgcggcg                                                            <  2:6655672/93‑1 (MQ=255)

                               gACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTa                                                          <  1:7549514/93‑1 (MQ=255)

                                aCGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTaa                                                         <  2:298018/93‑1 (MQ=255)

                                 cGCACAAGACGATCTGGTGCCGTCAATCCAGGACGAAGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTaaa                                                        <  1:7154187/93‑1 (MQ=255)

                                   cacaAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACg                                                      >  2:4062998/1‑93 (MQ=255)

                                    acaAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGc                                                     <  1:1039043/93‑1 (MQ=255)

                                     caAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGcc                                                    >  2:2913701/1‑93 (MQ=255)

                                     caAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGcc                                                    >  2:2270220/1‑93 (MQ=255)

                                       aGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCtt                                                  <  1:403875/93‑1 (MQ=255)

                                       aGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCtt                                                  >  1:4321858/1‑93 (MQ=255)

                                       aGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCtt                                                  >  1:3638971/1‑93 (MQ=255)

                                        gACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGTTATTGAGATGCCGGATGCGGCGTAAACGCCTTa                                                 >  1:3961270/1‑93 (MQ=255)

                                        gACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTa                                                 <  2:8306745/93‑1 (MQ=255)

                                         aCGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTAt                                                <  2:7003828/93‑1 (MQ=255)

                                         aCGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTAt                                                <  2:6398140/93‑1 (MQ=255)

                                         aCGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTAt                                                >  2:3408748/1‑93 (MQ=255)

                                          cGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATc                                               <  1:4367725/93‑1 (MQ=255)

                                          cGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATc                                               <  1:8680553/93‑1 (MQ=255)

                                          cGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCACGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATc                                               <  1:916700/93‑1 (MQ=255)

                                           taTCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATcc                                              <  2:9310769/92‑1 (MQ=255)

                                           gATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATcc                                              <  2:8117612/93‑1 (MQ=255)

                                           gATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATcc                                              <  2:8739737/93‑1 (MQ=255)

                                           gATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATcc                                              <  2:650820/93‑1 (MQ=255)

                                             tCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCgg                                            <  2:4819296/93‑1 (MQ=255)

                                              cTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGc                                           <  1:1378391/93‑1 (MQ=255)

                                                 gTGCCGACAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGCAAACGCCTTATCCGGCCtc                                        >  2:1508434/1‑92 (MQ=38)

                                                   gCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACg                                      <  2:5586461/93‑1 (MQ=255)

                                                      gTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCt                                   <  2:7130824/93‑1 (MQ=255)

                                                       tCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTc                                  >  1:4453273/1‑93 (MQ=255)

                                                          aTCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGt                               >  1:8043687/1‑93 (MQ=255)

                                                           tCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGtt                              <  1:7955572/93‑1 (MQ=255)

                                                            ccAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGttt                             <  1:3743688/93‑1 (MQ=255)

                                                             cAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTg                            >  1:9745691/1‑93 (MQ=255)

                                                             cAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGATTg                            >  2:3238167/1‑93 (MQ=255)

                                                              aGGGCGATGGCTGCGAAAGCTGCACATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTGATCCGGCCTACGGCGCGGTTTGt                           <  2:3440499/93‑1 (MQ=25)

                                                               ggACGATGGCTGCGTGCGCGGCGCTTGCGTGCTCTGATGTTGCGATGCCGGCTGCGGCGTCCACGCCTTGTCCGGCCTCCGGCTCGGTTTGTa                          <  1:7686908/93‑1 (MQ=16)

                                                               ggACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTa                          >  1:8265904/1‑93 (MQ=255)

                                                               ggACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCCGTTTGTa                          >  1:8938469/1‑93 (MQ=255)

                                                                 aCGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAgg                        >  2:3160321/1‑93 (MQ=255)

                                                                  cGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGc                       >  1:4567865/1‑93 (MQ=255)

                                                                   gATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGcc                      <  1:6190132/93‑1 (MQ=255)

                                                                   gATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGcc                      >  2:2030672/1‑93 (MQ=255)

                                                                   gATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGcc                      <  2:9748418/93‑1 (MQ=255)

                                                                    aTGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAAGCCt                     >  2:282731/1‑93 (MQ=255)

                                                                       gCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGAt                  <  2:1842866/93‑1 (MQ=255)

                                                                       gCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGAt                  >  2:761367/1‑93 (MQ=255)

                                                                       gCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGAt                  >  1:1326853/1‑93 (MQ=255)

                                                                        cTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATa                 <  2:3539459/93‑1 (MQ=255)

                                                                         tGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATaa                <  1:3909072/93‑1 (MQ=255)

                                                                         tGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATaa                <  2:19226/93‑1 (MQ=255)

                                                                         tGCGAAAGCGGCGCATGCAAGATATGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATaa                <  2:5539981/93‑1 (MQ=255)

                                                                            gAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAc             >  2:8421384/1‑93 (MQ=255)

                                                                            gAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAc             <  1:6035223/93‑1 (MQ=255)

                                                                             aaaGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAcg            <  1:8352953/93‑1 (MQ=255)

                                                                             aaaGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAcg            <  1:3471642/93‑1 (MQ=255)

                                                                              aaGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAcgc           >  1:5970042/1‑93 (MQ=255)

                                                                                  ggcgCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCa       <  2:1055066/93‑1 (MQ=255)

                                                                                  ggcgCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCa       <  2:5269520/93‑1 (MQ=255)

                                                                                  ggcgCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCa       <  2:6177933/93‑1 (MQ=255)

                                                                                    cgcATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTCTCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGc     <  2:4773816/93‑1 (MQ=255)

                                                                                     gcATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCg    <  2:4284752/93‑1 (MQ=255)

                                                                                      cATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGt   >  1:9788202/1‑93 (MQ=255)

                                                                                       aTGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTc  >  1:940176/1‑93 (MQ=255)

GTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGTAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTC  >  NC_000913/2345071‑2345250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACTGTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGTAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCAT  >  NC_000913/2345068‑2345254

ACTGTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGC                                                                                               <  SRR4302540.8470669/94‑1 (MQ=60)

ACTGTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGC                                                                                               <  SRR4302540.5652472/94‑1 (MQ=60)

 CTGTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCA                                                                                              >  SRR4302540.3160802/1‑94 (MQ=60)

 CTGTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCA                                                                                              <  SRR4302540.6281381/94‑1 (MQ=60)

 CTGTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCA                                                                                              >  SRR4302540.4541335/1‑94 (MQ=60)

 CTGTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCA                                                                                              >  SRR4302540.297670/1‑94 (MQ=60)

  TGTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAA                                                                                             >  SRR4302540.4084130/1‑94 (MQ=60)

  TGTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAA                                                                                             <  SRR4302540.9516840/94‑1 (MQ=60)

   GTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAG                                                                                            <  SRR4302540.4586151/94‑1 (MQ=60)

    TATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGA                                                                                           >  SRR4302540.8718046/1‑94 (MQ=60)

      TTATCAGACCACCCGTGACGGCGCTGAAGCCGCGCAAGGCGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATC                                                                                         <  SRR4302540.606661/94‑1 (MQ=60)

      TTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATC                                                                                         <  SRR4302540.5964484/94‑1 (MQ=60)

      TTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATC                                                                                         <  SRR4302540.9847267/94‑1 (MQ=60)

       TATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCT                                                                                        <  SRR4302540.6424539/94‑1 (MQ=60)

       TATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCT                                                                                        <  SRR4302540.3293857/94‑1 (MQ=60)

         TCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGA                                                                                      <  SRR4302540.9384979/94‑1 (MQ=60)

         TCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGA                                                                                      >  SRR4302540.3549230/1‑94 (MQ=60)

         TCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGAGGCGCATGCAAGCTCTGA                                                                                      >  SRR4302540.3549229/1‑94 (MQ=60)

          CAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGAT                                                                                     >  SRR4302540.2250443/1‑94 (MQ=60)

            GAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATAT                                                                                   <  SRR4302540.8654610/94‑1 (MQ=60)

            GAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATAT                                                                                   >  SRR4302540.7663115/1‑94 (MQ=60)

             AACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATT                                                                                  <  SRR4302540.6323826/94‑1 (MQ=60)

              ACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTG                                                                                 >  SRR4302540.7762811/1‑94 (MQ=60)

                ACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAG                                                                               <  SRR4302540.6722773/94‑1 (MQ=60)

                  CCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGAT                                                                             >  SRR4302540.4510392/1‑94 (MQ=60)

                  CCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGAT                                                                             <  SRR4302540.4354187/94‑1 (MQ=60)

                  CCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGAT                                                                             <  SRR4302540.4582057/94‑1 (MQ=60)

                  CCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGAT                                                                             >  SRR4302540.2130235/1‑94 (MQ=60)

                  CCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGAT                                                                             >  SRR4302540.9129937/1‑94 (MQ=60)

                  CCGTGACGGCCCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGAT                                                                             <  SRR4302540.3060421/94‑1 (MQ=60)

                     TGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCC                                                                          <  SRR4302540.4899082/94‑1 (MQ=60)

                     TGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCC                                                                          <  SRR4302540.4899076/94‑1 (MQ=60)

                       GCGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGG                                                                        <  SRR4302540.3960128/94‑1 (MQ=60)

                       ACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGG                                                                        >  SRR4302540.9105666/1‑94 (MQ=60)

                         GGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGAT                                                                      <  SRR4302540.3126243/94‑1 (MQ=60)

                         GGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGAT                                                                      >  SRR4302540.6105928/1‑94 (MQ=60)

                         GGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGAT                                                                      >  SRR4302540.9861786/1‑94 (MQ=60)

                         GGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGAT                                                                      <  SRR4302540.4790110/94‑1 (MQ=60)

                         GGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGAT                                                                      <  SRR4302540.550435/94‑1 (MQ=60)

                           CGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGC                                                                    <  SRR4302540.6047336/94‑1 (MQ=60)

                            GCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCG                                                                   >  SRR4302540.2358059/1‑94 (MQ=60)

                            GCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCG                                                                   >  SRR4302540.2149485/1‑94 (MQ=60)

                              TGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCCAGATCTGATATTGAGATGCCGGATGCGGC                                                                 >  SRR4302540.4839903/1‑94 (MQ=60)

                              TGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGC                                                                 >  SRR4302540.4839902/1‑94 (MQ=60)

                              TGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGC                                                                 <  SRR4302540.7431767/94‑1 (MQ=60)

                               GAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCG                                                                >  SRR4302540.9496705/1‑94 (MQ=60)

                               GAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCG                                                                <  SRR4302540.6892239/94‑1 (MQ=60)

                               GAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCG                                                                <  SRR4302540.2855352/94‑1 (MQ=60)

                                 AGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTA                                                              <  SRR4302540.7154472/94‑1 (MQ=60)

                                  GACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAA                                                             <  SRR4302540.7634134/94‑1 (MQ=60)

                                   ACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAA                                                            >  SRR4302540.6469568/1‑94 (MQ=60)

                                    CGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAAC                                                           <  SRR4302540.4358241/94‑1 (MQ=60)

                                    CGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAAC                                                           <  SRR4302540.6952358/94‑1 (MQ=60)

                                    CGCACAAGACGATCTGGTGCCGTCAATCCAGGACGAAGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAAC                                                           <  SRR4302540.7233918/94‑1 (MQ=60)

                                      CACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGC                                                         <  SRR4302540.6551167/94‑1 (MQ=60)

                                       ACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCC                                                        <  SRR4302540.1049608/94‑1 (MQ=60)

                                       ACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCC                                                        <  SRR4302540.8536906/94‑1 (MQ=60)

                                       ACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCC                                                        >  SRR4302540.9494914/1‑94 (MQ=60)

                                         AAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTT                                                      >  SRR4302540.4368913/1‑94 (MQ=60)

                                         AAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTT                                                      >  SRR4302540.3678815/1‑94 (MQ=60)

                                          AGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGTTATTGAGATGCCGGATGCGGCGTAAACGCCTTA                                                     >  SRR4302540.4003993/1‑94 (MQ=60)

                                          AGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTA                                                     <  SRR4302540.408091/94‑1 (MQ=60)

                                           GACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTAT                                                    <  SRR4302540.827920/94‑1 (MQ=60)

                                           GACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTAT                                                    <  SRR4302540.2907119/94‑1 (MQ=60)

                                             CGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCC                                                  <  SRR4302540.4415186/94‑1 (MQ=60)

                                             CGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCC                                                  <  SRR4302540.8781365/94‑1 (MQ=60)

                                             CGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCACGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCC                                                  <  SRR4302540.926380/94‑1 (MQ=60)

                                              GATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCG                                                 <  SRR4302540.8842458/94‑1 (MQ=60)

                                               ATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGG                                                >  SRR4302540.843345/1‑94 (MQ=60)

                                               ATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGG                                                <  SRR4302540.9563780/94‑1 (MQ=60)

                                                 CTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCC                                              >  SRR4302540.3604188/1‑94 (MQ=60)

                                                 CTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCC                                              <  SRR4302540.1393072/94‑1 (MQ=60)

                                                 CTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCC                                              <  SRR4302540.6237148/94‑1 (MQ=60)

                                                    GTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAC                                           <  SRR4302540.1113254/94‑1 (MQ=60)

                                                        CGTGAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGACATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCT                                       <  SRR4302540.4388074/94‑1 (MQ=60)

                                                        CGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCT                                       <  SRR4302540.4023739/94‑1 (MQ=60)

                                                        CGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCT                                       >  SRR4302540.1815667/1‑94 (MQ=60)

                                                         GTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTC                                      <  SRR4302540.6797659/94‑1 (MQ=60)

                                                         GTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTC                                      >  SRR4302540.4501548/1‑94 (MQ=60)

                                                         GTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTC                                      <  SRR4302540.4478633/94‑1 (MQ=60)

                                                          TCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCG                                     >  SRR4302540.3841380/1‑94 (MQ=60)

                                                          TCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCG                                     <  SRR4302540.10075110/94‑1 (MQ=60)

                                                           CAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG                                    >  SRR4302540.6726992/1‑94 (MQ=60)

                                                           CAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG                                    <  SRR4302540.761141/94‑1 (MQ=60)

                                                           CAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG                                    >  SRR4302540.5006924/1‑94 (MQ=60)

                                                           CAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGG                                    >  SRR4302540.2035738/1‑94 (MQ=60)

                                                            AATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGT                                   >  SRR4302540.8949500/1‑94 (MQ=60)

                                                            AATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGT                                   >  SRR4302540.8137142/1‑94 (MQ=60)

                                                            AATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGT                                   >  SRR4302540.7862816/1‑94 (MQ=60)

                                                              TCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTT                                 <  SRR4302540.8779287/94‑1 (MQ=60)

                                                              TCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTT                                 <  SRR4302540.8048309/94‑1 (MQ=60)

                                                               CCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTT                                >  SRR4302540.282601/1‑94 (MQ=60)

                                                               CCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTG                                >  SRR4302540.3752013/1‑94 (MQ=60)

                                                               CCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTG                                >  SRR4302540.9860063/1‑94 (MQ=60)

                                                               CCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTG                                <  SRR4302540.3784546/94‑1 (MQ=60)

                                                                 AGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTA                              >  SRR4302540.8362262/1‑94 (MQ=60)

                                                                 AGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTA                              <  SRR4302540.4502340/94‑1 (MQ=60)

                                                                 AGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTA                              >  SRR4302540.8361950/1‑94 (MQ=60)

                                                                 AGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTA                              <  SRR4302540.8269290/94‑1 (MQ=60)

                                                                 AGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTA                              <  SRR4302540.8046814/94‑1 (MQ=60)

                                                                 AGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCCGTTTGTA                              >  SRR4302540.9043247/1‑94 (MQ=60)

                                                                  GGACGATGGCTGCTAAAGCGGCGCATGCAAGATCTGATACTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAG                             <  SRR4302540.3887553/94‑1 (MQ=60)

                                                                  GGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAG                             >  SRR4302540.4498626/1‑94 (MQ=60)

                                                                    ACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGC                           >  SRR4302540.4617182/1‑94 (MQ=60)

                                                                      GATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCT                         >  SRR4302540.4079674/1‑94 (MQ=60)

                                                                      GATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCT                         <  SRR4302540.6259051/94‑1 (MQ=60)

                                                                       ATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTG                        >  SRR4302540.4807218/1‑94 (MQ=60)

                                                                       ATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTG                        <  SRR4302540.3527396/94‑1 (MQ=60)

                                                                         GGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGAT                      >  SRR4302540.1340702/1‑94 (MQ=60)

                                                                           CTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAA                    >  SRR4302540.3709359/1‑94 (MQ=60)

                                                                            TGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAG                   <  SRR4302540.3951440/94‑1 (MQ=60)

                                                                             GCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGA                  <  SRR4302540.1341220/94‑1 (MQ=60)

                                                                             GCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGA                  <  SRR4302540.3187905/94‑1 (MQ=60)

                                                                              CGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAC                 <  SRR4302540.7459517/94‑1 (MQ=60)

                                                                              CGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAC                 <  SRR4302540.4404883/94‑1 (MQ=60)

                                                                               GAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACG                >  SRR4302540.1354116/1‑94 (MQ=60)

                                                                               GAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACG                >  SRR4302540.5397697/1‑94 (MQ=60)

                                                                               GAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACG                >  SRR4302540.9327175/1‑94 (MQ=60)

                                                                               GAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACG                <  SRR4302540.6102214/94‑1 (MQ=60)

                                                                               GAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGGTTGTAGGGCTGGTAAGACC                >  SRR4302540.4206791/1‑94 (MQ=60)

                                                                                AAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGC               <  SRR4302540.8449897/94‑1 (MQ=60)

                                                                                AAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGC               <  SRR4302540.3509440/94‑1 (MQ=60)

                                                                                AAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGC               >  SRR4302540.6036120/1‑94 (MQ=60)

                                                                                AAACCGGCGCATGCAAGATCTGATATTGAGATCCCGCATGCGGCGTAAACGCCTTACCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGC               <  SRR4302540.3509441/94‑1 (MQ=60)

                                                                                    CGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGGTAAGACGCGCCC           >  SRR4302540.7400156/1‑94 (MQ=60)

                                                                                    CGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCA           <  SRR4302540.8119473/94‑1 (MQ=60)

                                                                                       CGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCG        >  SRR4302540.9870698/1‑94 (MQ=60)

                                                                                        GCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGT       >  SRR4302540.9902879/1‑94 (MQ=60)

                                                                                         CATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTC      >  SRR4302540.950005/1‑94 (MQ=60)

                                                                                          ATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCG     >  SRR4302540.10061071/1‑94 (MQ=60)

                                                                                          ATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGGTTGTAGGCCTGATAAGACGCGCCAGCGTCG     >  SRR4302540.2490057/1‑94 (MQ=60)

                                                                                            GCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCA   <  SRR4302540.2830792/94‑1 (MQ=60)

                                                                                            GCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCA   >  SRR4302540.3920792/1‑94 (MQ=60)

                                                                                             CAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCAT  >  SRR4302540.8873468/1‑94 (MQ=60)

                                                                                             CAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCAT  >  SRR4302540.2716176/1‑94 (MQ=60)

                                                                                             CAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCAT  >  SRR4302540.7600440/1‑94 (MQ=60)

ACTGTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGTAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCAT  >  NC_000913/2345068‑2345254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`