Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,345,161 T→C C759C (TGT→TGC)  nrdA → ribonucleoside diphosphate reductase 1 alpha subunit, B1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,345,1610TC100.0% 826.5 / NA 251C759C (TGT→TGC) nrdAribonucleoside diphosphate reductase 1 alpha subunit, B1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (124/127);  total (124/127)

Only 100 of 257 total aligned reads displayed.

GTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGTAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTC  >  NC_000913/2345071‑2345250
                                                                                          |                                                                                         
ttattatCAGAACCCCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTTCGAAAGCGGCGCATGCaa                                                                                         <  2:4319333/92‑1 (MQ=255)
gTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCaa                                                                                         >  2:5181735/1‑93 (MQ=255)
 tattatCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAg                                                                                        <  2:3548495/93‑1 (MQ=255)
 tattatCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAg                                                                                        >  1:3947834/1‑93 (MQ=255)
 tattatCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAg                                                                                        <  2:4723471/93‑1 (MQ=255)
  attatCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGa                                                                                       <  2:3766729/93‑1 (MQ=255)
  attatCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGa                                                                                       <  2:3190704/93‑1 (MQ=255)
   ttatCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGAt                                                                                      >  1:4138684/1‑93 (MQ=255)
    tatCAGACCACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATc                                                                                     <  2:2353521/93‑1 (MQ=255)
      tCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTg                                                                                   <  2:6076650/93‑1 (MQ=255)
      tCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTg                                                                                   <  1:1398867/93‑1 (MQ=255)
       cAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGa                                                                                  <  2:1265359/93‑1 (MQ=255)
       cAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGa                                                                                  <  1:3574106/93‑1 (MQ=255)
        aGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGat                                                                                 >  2:1737713/1‑93 (MQ=255)
         gAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGata                                                                                <  1:1680624/93‑1 (MQ=255)
         gAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGata                                                                                <  2:3301047/93‑1 (MQ=255)
          aaCACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGatat                                                                               <  1:530898/93‑1 (MQ=255)
           acacCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATAtt                                                                              >  2:5242715/1‑93 (MQ=255)
           acacCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATAtt                                                                              >  2:777208/1‑93 (MQ=255)
            cacCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTg                                                                             <  1:1110702/93‑1 (MQ=255)
               ccGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTgaga                                                                          >  1:680243/1‑93 (MQ=255)
               ccGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTgaga                                                                          <  1:850428/93‑1 (MQ=255)
               ccGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTgaga                                                                          <  1:4382489/93‑1 (MQ=255)
               ccGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTgaga                                                                          <  2:853621/93‑1 (MQ=255)
               ccGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTgaga                                                                          <  2:5659787/93‑1 (MQ=255)
                cGTGACGGCGCTGAAGACGCACCAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCCAGATCTGATATTGAGAt                                                                         >  2:4008911/1‑93 (MQ=255)
                cGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGAt                                                                         >  2:3648403/1‑93 (MQ=255)
                cGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGAt                                                                         >  1:3207880/1‑93 (MQ=255)
                  tGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGc                                                                       <  2:1387041/93‑1 (MQ=255)
                  tGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGc                                                                       >  2:1228480/1‑93 (MQ=255)
                  tGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGc                                                                       >  1:1779125/1‑93 (MQ=255)
                  tGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGc                                                                       >  1:3427301/1‑93 (MQ=255)
                       gcgcTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGAt                                                                  <  2:3820/93‑1 (MQ=255)
                        cgcTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATg                                                                 <  1:5291775/93‑1 (MQ=255)
                         gcTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgc                                                                >  2:2707894/1‑93 (MQ=255)
                         gcTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgc                                                                <  1:3911879/93‑1 (MQ=255)
                         gcTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgc                                                                <  2:2062067/93‑1 (MQ=255)
                          cTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgcg                                                               >  1:1027601/1‑93 (MQ=255)
                          cTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgcg                                                               >  2:1142965/1‑93 (MQ=255)
                           tGAAGACGCACAATACGATCTGGTGCCGTCAGTCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGGCGGATgcgg                                                              <  2:4524526/93‑1 (MQ=255)
                           tGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgcgg                                                              >  1:4345269/1‑93 (MQ=255)
                           tGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgcgg                                                              >  2:4731373/1‑93 (MQ=255)
                             aaGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATgcggcg                                                            >  2:3939192/1‑93 (MQ=255)
                                aCGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTaa                                                         <  1:4878345/93‑1 (MQ=255)
                                 cGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTaaa                                                        <  1:6003495/93‑1 (MQ=255)
                                 cGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTaaa                                                        <  1:604974/93‑1 (MQ=255)
                                  gCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAAc                                                       >  2:2217851/1‑93 (MQ=255)
                                  gCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAAc                                                       >  1:4439379/1‑93 (MQ=255)
                                   cacaAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACg                                                      >  2:1030560/1‑93 (MQ=255)
                                     caAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAGGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGcc                                                    >  2:1113294/1‑93 (MQ=255)
                                     caAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGcc                                                    <  1:150177/93‑1 (MQ=255)
                                     caAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGcc                                                    <  2:3379838/93‑1 (MQ=255)
                                     caAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGcc                                                    <  1:5884789/93‑1 (MQ=255)
                                      aaGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCt                                                   >  1:4606685/1‑93 (MQ=255)
                                       aGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCtt                                                  >  2:4870714/1‑93 (MQ=255)
                                        gACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTa                                                 <  1:1748858/93‑1 (MQ=255)
                                         aCGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATTCCGGATGCGGCGTAAACGCCTTAt                                                >  1:571972/1‑93 (MQ=255)
                                         aCGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTAt                                                >  1:5097087/1‑93 (MQ=255)
                                          cGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATc                                               <  1:4896778/93‑1 (MQ=255)
                                          cGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATc                                               <  2:6274029/93‑1 (MQ=255)
                                            aTCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCg                                             >  2:699981/1‑93 (MQ=255)
                                            aTCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCCTAAACGCCTTATCCg                                             <  1:3105077/93‑1 (MQ=255)
                                              cTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGc                                           >  1:2483703/1‑93 (MQ=255)
                                              cTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGc                                           <  1:2241458/93‑1 (MQ=255)
                                               tGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                          <  1:405696/93‑1 (MQ=255)
                                               tGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGcc                                          >  1:5505259/1‑93 (MQ=255)
                                                ggTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                         <  1:5820578/93‑1 (MQ=255)
                                                ggTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                         <  2:368187/93‑1 (MQ=255)
                                                ggTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                         >  2:435434/1‑93 (MQ=255)
                                                ggTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                         <  2:4047418/93‑1 (MQ=255)
                                                   gCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACg                                      <  2:5937677/93‑1 (MQ=255)
                                                    ccGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACgg                                     <  1:6103333/93‑1 (MQ=255)
                                                     cGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGc                                    <  1:200966/93‑1 (MQ=255)
                                                     cGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGc                                    >  2:3616615/1‑93 (MQ=255)
                                                      gTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCt                                   >  2:1711420/1‑93 (MQ=255)
                                                       tCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACTCCTTATCCGGCCTACGGCTc                                  <  1:4942255/93‑1 (MQ=255)
                                                         aaTCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCgg                                <  2:536088/93‑1 (MQ=255)
                                                          aTCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGt                               <  1:5464007/93‑1 (MQ=255)
                                                               ggACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTa                          <  2:4624816/93‑1 (MQ=255)
                                                                  cGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTCGGc                       >  2:2895292/1‑93 (MQ=255)
                                                                      ggCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGa                   <  1:3927346/93‑1 (MQ=255)
                                                                      ggCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGa                   <  1:5220174/93‑1 (MQ=255)
                                                                         tGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATaa                <  1:329561/93‑1 (MQ=255)
                                                                          gCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATCAg               >  2:2736581/1‑93 (MQ=255)
                                                                            gAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGGCTGATAAGAc             >  2:4129634/1‑93 (MQ=255)
                                                                            caaaGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAc             <  2:1409350/92‑1 (MQ=255)
                                                                             aaaGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAcg            <  1:2137637/93‑1 (MQ=255)
                                                                              aaGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAcgc           <  2:1486914/93‑1 (MQ=255)
                                                                              aaGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAcgc           <  1:279832/93‑1 (MQ=255)
                                                                               aGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAcgcg          >  1:2971359/1‑93 (MQ=255)
                                                                               aGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAcgcg          <  2:586534/93‑1 (MQ=255)
                                                                                gcggcgCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACgcgc         >  1:251677/1‑93 (MQ=255)
                                                                                gcggcgCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAAgcgc         >  1:2645920/1‑93 (MQ=255)
                                                                                  ggcgCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCa       >  1:3635011/1‑93 (MQ=255)
                                                                                  ggcgCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCa       <  1:3294912/93‑1 (MQ=255)
                                                                                  ggcgCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAAGCGCCa       >  2:251718/1‑93 (MQ=255)
                                                                                     gcATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCg    >  2:3274787/1‑93 (MQ=255)
                                                                                     gcATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCg    >  2:4598059/1‑93 (MQ=255)
                                                                                      cATGCAAGACCTGATATTGAGATGCCGGATGCGGCATACACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGt   <  1:1852351/93‑1 (MQ=18)
                                                                                       aTGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTc  <  1:6148549/93‑1 (MQ=255)
                                                                                          |                                                                                         
GTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGTAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTC  >  NC_000913/2345071‑2345250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTGTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGTAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCAT  >  NC_000913/2345068‑2345254
                                                                                             |                                                                                             
ACTGTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGC                                                                                               >  SRR4302533.1007247/1‑94 (MQ=60)
 CTGTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCA                                                                                              <  SRR4302533.5991772/94‑1 (MQ=60)
 CTGTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCA                                                                                              >  SRR4302533.1574203/1‑94 (MQ=60)
  TGTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAA                                                                                             <  SRR4302533.75044/94‑1 (MQ=60)
   GTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAG                                                                                            >  SRR4302533.3150477/1‑94 (MQ=60)
   GTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAG                                                                                            >  SRR4302533.3971910/1‑94 (MQ=60)
   GTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAG                                                                                            >  SRR4302533.2918397/1‑94 (MQ=60)
   GTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAG                                                                                            >  SRR4302533.3971909/1‑94 (MQ=60)
    TATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGA                                                                                           <  SRR4302533.4099412/94‑1 (MQ=60)
     ATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGAT                                                                                          >  SRR4302533.4163789/1‑94 (MQ=60)
      TTATCAGAACCCCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATC                                                                                         <  SRR4302533.1786517/94‑1 (MQ=60)
      TTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATC                                                                                         <  SRR4302533.2023876/94‑1 (MQ=60)
        ATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTG                                                                                       >  SRR4302533.3498026/1‑94 (MQ=60)
        ATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTG                                                                                       <  SRR4302533.3311007/94‑1 (MQ=60)
         TCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGA                                                                                      <  SRR4302533.1407298/94‑1 (MQ=60)
          CAGAACCCCCGGGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGAT                                                                                     <  SRR4302533.1124151/94‑1 (MQ=60)
          CAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGAT                                                                                     <  SRR4302533.3596136/94‑1 (MQ=60)
          CAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGAT                                                                                     <  SRR4302533.343665/94‑1 (MQ=60)
          CAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGAT                                                                                     >  SRR4302533.4402886/1‑94 (MQ=60)
            GAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATAT                                                                                   <  SRR4302533.1690806/94‑1 (MQ=60)
             AACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATT                                                                                  >  SRR4302533.5979522/1‑94 (MQ=60)
             AACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATT                                                                                  <  SRR4302533.534056/94‑1 (MQ=60)
              ACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTG                                                                                 <  SRR4302533.5145315/94‑1 (MQ=60)
               CACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGA                                                                                <  SRR4302533.4067726/94‑1 (MQ=60)
               CACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGA                                                                                >  SRR4302533.3071360/1‑94 (MQ=60)
               CACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGA                                                                                <  SRR4302533.5144981/94‑1 (MQ=60)
               CACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGA                                                                                <  SRR4302533.1117291/94‑1 (MQ=60)
                 CCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGA                                                                              >  SRR4302533.684266/1‑94 (MQ=60)
                  CCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGAT                                                                             >  SRR4302533.3227634/1‑94 (MQ=60)
                  CCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGAT                                                                             <  SRR4302533.4408948/94‑1 (MQ=60)
                  CCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGAT                                                                             <  SRR4302533.608676/94‑1 (MQ=60)
                  CCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGAT                                                                             <  SRR4302533.855405/94‑1 (MQ=60)
                  CCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGAT                                                                             <  SRR4302533.2272034/94‑1 (MQ=60)
                   CGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATG                                                                            >  SRR4302533.1399762/1‑94 (MQ=60)
                    GTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGC                                                                           >  SRR4302533.1789957/1‑94 (MQ=60)
                    GTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGC                                                                           <  SRR4302533.3728633/94‑1 (MQ=60)
                    GTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGC                                                                           <  SRR4302533.3619184/94‑1 (MQ=60)
                    GTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGC                                                                           >  SRR4302533.1667891/1‑94 (MQ=60)
                    GTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGC                                                                           >  SRR4302533.3448472/1‑94 (MQ=60)
                      GACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCG                                                                         <  SRR4302533.5252738/94‑1 (MQ=60)
                        CGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGA                                                                       <  SRR4302533.3298526/94‑1 (MQ=60)
                         GGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGAT                                                                      <  SRR4302533.4243104/94‑1 (MQ=60)
                         GGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGAT                                                                      >  SRR4302533.825868/1‑94 (MQ=60)
                          GCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATG                                                                     <  SRR4302533.3632084/94‑1 (MQ=60)
                          GCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATG                                                                     <  SRR4302533.65185/94‑1 (MQ=60)
                           CGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGC                                                                    >  SRR4302533.5899129/1‑94 (MQ=60)
                           CGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGC                                                                    <  SRR4302533.5323457/94‑1 (MQ=60)
                            GCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCG                                                                   <  SRR4302533.3935769/94‑1 (MQ=60)
                            GCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCG                                                                   >  SRR4302533.1033636/1‑94 (MQ=60)
                             CTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGG                                                                  >  SRR4302533.4371456/1‑94 (MQ=60)
                             CTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGG                                                                  >  SRR4302533.5520947/1‑94 (MQ=60)
                              TGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGC                                                                 >  SRR4302533.223578/1‑94 (MQ=60)
                              TGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGC                                                                 >  SRR4302533.2320288/1‑94 (MQ=60)
                              TGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGC                                                                 <  SRR4302533.1810824/94‑1 (MQ=60)
                                AAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGT                                                               >  SRR4302533.2612356/1‑94 (MQ=60)
                                 AGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTA                                                              <  SRR4302533.6086524/94‑1 (MQ=60)
                                   ACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAA                                                            >  SRR4302533.261026/1‑94 (MQ=60)
                                   ACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAA                                                            <  SRR4302533.4907899/94‑1 (MQ=60)
                                    CGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAAC                                                           >  SRR4302533.4466214/1‑94 (MQ=60)
                                    CGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAAC                                                           <  SRR4302533.608527/94‑1 (MQ=60)
                                    CGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAAC                                                           <  SRR4302533.6039871/94‑1 (MQ=60)
                                     GCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACG                                                          <  SRR4302533.88624/94‑1 (MQ=60)
                                     GCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACG                                                          >  SRR4302533.4596660/1‑94 (MQ=60)
                                      CACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGC                                                         >  SRR4302533.5343987/1‑94 (MQ=60)
                                        CAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCT                                                       <  SRR4302533.5920351/94‑1 (MQ=60)
                                        CAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCT                                                       <  SRR4302533.5877483/94‑1 (MQ=60)
                                        CAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCT                                                       >  SRR4302533.4634588/1‑94 (MQ=60)
                                        CAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCT                                                       <  SRR4302533.151142/94‑1 (MQ=60)
                                         AAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTT                                                      <  SRR4302533.4414723/94‑1 (MQ=60)
                                           GACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATTCCGGATGCGGCGTAAACGCCTTAT                                                    >  SRR4302533.575348/1‑94 (MQ=60)
                                           GACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTAT                                                    <  SRR4302533.1759505/94‑1 (MQ=60)
                                           GACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTAT                                                    >  SRR4302533.5127881/1‑94 (MQ=60)
                                           GACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTAT                                                    >  SRR4302533.2528621/1‑94 (MQ=60)
                                           GACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTAT                                                    >  SRR4302533.4665217/1‑94 (MQ=60)
                                             CGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCC                                                  <  SRR4302533.4948517/94‑1 (MQ=60)
                                             CGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCC                                                  >  SRR4302533.1098502/1‑94 (MQ=60)
                                             CGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCC                                                  <  SRR4302533.4926446/94‑1 (MQ=60)
                                               ATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCCTAAACGCCTTATCCGG                                                <  SRR4302533.3124164/94‑1 (MQ=60)
                                                TCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGC                                               >  SRR4302533.2499014/1‑94 (MQ=60)
                                                 CTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCC                                              <  SRR4302533.2255314/94‑1 (MQ=60)
                                                 CTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCC                                              >  SRR4302533.5538477/1‑94 (MQ=60)
                                                 CTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCC                                              >  SRR4302533.3936159/1‑94 (MQ=60)
                                                 CTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCC                                              <  SRR4302533.600768/94‑1 (MQ=60)
                                                  TGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCT                                             <  SRR4302533.408135/94‑1 (MQ=60)
                                                   GGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTA                                            <  SRR4302533.5855738/94‑1 (MQ=60)
                                                   GGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTA                                            >  SRR4302533.3906866/1‑94 (MQ=60)
                                                     TGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACG                                          >  SRR4302533.5334218/1‑94 (MQ=60)
                                                     TGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACG                                          <  SRR4302533.5764899/94‑1 (MQ=60)
                                                     TGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACG                                          <  SRR4302533.935712/94‑1 (MQ=60)
                                                     TGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACG                                          <  SRR4302533.396090/94‑1 (MQ=60)
                                                     TGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACG                                          >  SRR4302533.2091195/1‑94 (MQ=60)
                                                      GCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGG                                         >  SRR4302533.658218/1‑94 (MQ=60)
                                                       CCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGC                                        <  SRR4302533.6140175/94‑1 (MQ=60)
                                                       CCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGC                                        <  SRR4302533.3315638/94‑1 (MQ=60)
                                                        CGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCT                                       <  SRR4302533.1438779/94‑1 (MQ=60)
                                                        CGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCT                                       <  SRR4302533.202240/94‑1 (MQ=60)
                                                         GTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTC                                      >  SRR4302533.1771377/1‑94 (MQ=60)
                                                         GTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTC                                      >  SRR4302533.5289750/1‑94 (MQ=60)
                                                          TCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACTCCTTATCCGGCCTACGGCTCG                                     <  SRR4302533.4972172/94‑1 (MQ=60)
                                                             ATCCAGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTT                                  <  SRR4302533.5496933/94‑1 (MQ=60)
                                                                 AGGACGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTA                              <  SRR4302533.5747280/94‑1 (MQ=60)
                                                                     CGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCC                          >  SRR4302533.6073807/1‑94 (MQ=60)
                                                                     CGATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCC                          <  SRR4302533.4398244/94‑1 (MQ=60)
                                                                      GATGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCT                         >  SRR4302533.6166427/1‑94 (MQ=60)
                                                                        TGGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGA                       <  SRR4302533.162623/94‑1 (MQ=60)
                                                                         GGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCTGTTTGTAGGCCTGGT                      >  SRR4302533.2953963/1‑94 (MQ=60)
                                                                         GGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGAT                      >  SRR4302533.4151063/1‑94 (MQ=60)
                                                                         GGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGAT                      <  SRR4302533.5251591/94‑1 (MQ=60)
                                                                         GGCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGAT                      <  SRR4302533.3951321/94‑1 (MQ=60)
                                                                          GCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATA                     <  SRR4302533.5492142/94‑1 (MQ=60)
                                                                          GCTGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATA                     <  SRR4302533.4686502/94‑1 (MQ=60)
                                                                            TGCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAG                   <  SRR4302533.331573/94‑1 (MQ=60)
                                                                             GCGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGA                  <  SRR4302533.5014429/94‑1 (MQ=60)
                                                                              CGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAC                 >  SRR4302533.3318586/1‑94 (MQ=60)
                                                                              CGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAC                 >  SRR4302533.4448645/1‑94 (MQ=60)
                                                                              CGAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAC                 <  SRR4302533.2760139/94‑1 (MQ=60)
                                                                               GAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACG                >  SRR4302533.1880780/1‑94 (MQ=60)
                                                                               GAAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACG                <  SRR4302533.4932954/94‑1 (MQ=60)
                                                                                AAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGC               >  SRR4302533.5549987/1‑94 (MQ=60)
                                                                                AAAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGC               <  SRR4302533.2150820/94‑1 (MQ=60)
                                                                                 AAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCG              <  SRR4302533.281538/94‑1 (MQ=60)
                                                                                 AAGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCG              >  SRR4302533.2989533/1‑94 (MQ=60)
                                                                                  AGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGC             >  SRR4302533.253227/1‑94 (MQ=60)
                                                                                  AGCGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGAAGCGC             >  SRR4302533.2662179/1‑94 (MQ=60)
                                                                                    CGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCA           >  SRR4302533.4925745/1‑94 (MQ=60)
                                                                                    CGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCA           >  SRR4302533.3657365/1‑94 (MQ=60)
                                                                                    CGGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCA           <  SRR4302533.1898834/94‑1 (MQ=60)
                                                                                     GGCGCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAG          <  SRR4302533.3315247/94‑1 (MQ=60)
                                                                                        GCATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGGTTGTAGGCCTGATAAGACGCGCCAGCGT       >  SRR4302533.300658/1‑94 (MQ=60)
                                                                                         CATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTC      <  SRR4302533.1745461/94‑1 (MQ=60)
                                                                                         CATGCAAGACCTGATATTGAGATGCCGGATGCGGCATACACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTC      <  SRR4302533.1863646/94‑1 (MQ=57)
                                                                                          ATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCG     <  SRR4302533.6185563/94‑1 (MQ=60)
                                                                                          ATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCG     >  SRR4302533.1982343/1‑94 (MQ=60)
                                                                                          ATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCG     >  SRR4302533.4814753/1‑94 (MQ=60)
                                                                                          ATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCG     >  SRR4302533.1322507/1‑94 (MQ=60)
                                                                                          ATGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGGTTGTAGGCCTGATAAGAAGCGCCAGCGTCG     >  SRR4302533.2580775/1‑94 (MQ=60)
                                                                                           TGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCGC    <  SRR4302533.5878698/94‑1 (MQ=60)
                                                                                           TGCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCGC    >  SRR4302533.3168529/1‑94 (MQ=60)
                                                                                            GCAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCA   <  SRR4302533.5630571/94‑1 (MQ=60)
                                                                                             CAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGGTTGTAGGCCTGATAAGACGCGCCAGCGTCGCAT  >  SRR4302533.5398865/1‑94 (MQ=60)
                                                                                             |                                                                                             
ACTGTATTATCAGAACACCCGTGACGGCGCTGAAGACGCACAAGACGATCTGGTGCCGTCAATCCAGGACGATGGCTGCGAAAGCGGCGCATGTAAGATCTGATATTGAGATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGCTCGGTTTGTAGGCCTGATAAGACGCGCCAGCGTCGCAT  >  NC_000913/2345068‑2345254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑