Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,372,646 T→C A126A (GCA→GCG)  menE ← o‑succinylbenzoate‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,372,6460TC100.0% 671.7 / NA 208A126A (GCA→GCG) menEo‑succinylbenzoate‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (85/123);  total (85/123)

Only 100 of 228 total aligned reads displayed.

ATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATT  >  NC_000913/2372556‑2372735
                                                                                          |                                                                                         
aTAAGTATGGACAGGGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATgcgc                                                                                         <  2:1018214/93‑1 (MQ=255)
aTAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATgcgc                                                                                         >  2:7677177/1‑93 (MQ=255)
aTAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGGAGCGGCATgcgc                                                                                         >  2:3187935/1‑93 (MQ=255)
  aaGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATgcgcgc                                                                                       <  1:6258931/93‑1 (MQ=255)
     tATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCtt                                                                                    <  1:3864772/93‑1 (MQ=255)
      aTGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTc                                                                                   <  1:6690839/93‑1 (MQ=255)
       tGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCa                                                                                  >  2:598402/1‑93 (MQ=255)
       tGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCa                                                                                  >  2:1837641/1‑93 (MQ=255)
         gACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAAc                                                                                >  1:4749829/1‑93 (MQ=255)
          aCAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAAcc                                                                               <  1:3737251/93‑1 (MQ=255)
             gCGGCTTTCGGCAAACCGGTAGAACCCGCGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCCCGTCGCGGCATGAGCACCTTCACCCAGc                                                                            >  1:2008515/1‑93 (MQ=255)
             gCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGc                                                                            <  2:9566434/93‑1 (MQ=255)
             gCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGc                                                                            >  1:8522217/1‑93 (MQ=255)
             gCGGCTTCCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGc                                                                            <  2:6341230/93‑1 (MQ=255)
              cGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCt                                                                           <  1:3358894/93‑1 (MQ=255)
                gCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCCACCAGCTGa                                                                         >  2:9336678/1‑93 (MQ=255)
                gCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGa                                                                         <  1:6760119/93‑1 (MQ=255)
                gCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGa                                                                         >  1:8125976/1‑93 (MQ=255)
                gCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCCTCAACCCGCTGa                                                                         >  2:9026481/1‑93 (MQ=255)
                 cTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGaa                                                                        <  1:9160093/93‑1 (MQ=255)
                 cTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGaa                                                                        >  2:3184394/1‑93 (MQ=255)
                 cTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGaa                                                                        <  1:7933844/93‑1 (MQ=255)
                  tttCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAAt                                                                       >  2:4764411/1‑93 (MQ=255)
                   ttCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAAtg                                                                      <  2:4615166/93‑1 (MQ=255)
                    tCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAAtgt                                                                     >  1:2721191/1‑93 (MQ=255)
                     cGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAAtgtg                                                                    <  2:5571589/93‑1 (MQ=255)
                     cGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGATGAAtgtg                                                                    >  1:9040018/1‑93 (MQ=255)
                      ggCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGc                                                                   <  2:2688016/93‑1 (MQ=255)
                      ggCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGc                                                                   >  2:6881209/1‑93 (MQ=255)
                      ggCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGc                                                                   <  1:4649592/93‑1 (MQ=255)
                      ggCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGc                                                                   <  1:4370024/93‑1 (MQ=255)
                        cAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAg                                                                 >  2:2463537/1‑93 (MQ=255)
                        cAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAg                                                                 <  1:3455579/93‑1 (MQ=255)
                         aaaCCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGc                                                                <  1:5701547/93‑1 (MQ=255)
                          aaCCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCg                                                               <  1:2525690/93‑1 (MQ=255)
                              ggTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGt                                                           >  1:3923475/1‑93 (MQ=255)
                               gTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGtt                                                          <  2:3989862/93‑1 (MQ=255)
                                 aGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTaa                                                        <  1:8227020/93‑1 (MQ=255)
                                 aGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTaa                                                        <  1:8614053/93‑1 (MQ=255)
                                 aGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTaa                                                        <  1:5971250/93‑1 (MQ=255)
                                 aGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTaa                                                        <  2:8547488/93‑1 (MQ=255)
                                 aGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTaa                                                        <  1:3637345/93‑1 (MQ=255)
                                  gAACCCGAGGTCAACGTCATTGAGCGCAGACGCGTCGCCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAAt                                                       <  1:5712702/93‑1 (MQ=255)
                                  gAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAAt                                                       <  1:2038270/93‑1 (MQ=255)
                                  gAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAAt                                                       <  1:294355/93‑1 (MQ=255)
                                  gAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAAt                                                       <  1:5438234/93‑1 (MQ=255)
                                  gAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAAt                                                       >  2:5724267/1‑93 (MQ=255)
                                     cccGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGcc                                                    <  1:10030629/93‑1 (MQ=255)
                                       cGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCgg                                                  >  2:6095664/1‑93 (MQ=255)
                                        gAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGa                                                 <  2:9175221/93‑1 (MQ=255)
                                           gTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAAc                                              <  2:9339053/93‑1 (MQ=255)
                                             cAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACgt                                            <  1:4843593/93‑1 (MQ=255)
                                             cAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACgt                                            <  2:172168/93‑1 (MQ=255)
                                             cAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACgt                                            <  1:746888/93‑1 (MQ=255)
                                             cAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACgg                                            >  1:8343245/1‑92 (MQ=255)
                                              aaCGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACgtg                                           <  1:5490257/93‑1 (MQ=255)
                                              aaCGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACgtg                                           >  2:8659772/1‑93 (MQ=255)
                                              aaCGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACgtg                                           >  2:3475315/1‑93 (MQ=255)
                                               aCGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACgtgt                                          <  1:404719/93‑1 (MQ=255)
                                               aCGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACgtgt                                          >  2:3930034/1‑93 (MQ=255)
                                                cGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGtt                                         >  2:8328856/1‑93 (MQ=255)
                                                cGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGtt                                         <  1:3144583/93‑1 (MQ=255)
                                                cGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGtt                                         <  1:1686577/93‑1 (MQ=255)
                                                  tCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGtttt                                       >  1:4941690/1‑93 (MQ=255)
                                                  tCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGtttt                                       <  1:9355261/93‑1 (MQ=255)
                                                    aTTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTcc                                     >  2:4564034/1‑93 (MQ=255)
                                                     ttGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTccc                                    >  2:9913197/1‑93 (MQ=255)
                                                     ttGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTccc                                    <  1:1814623/93‑1 (MQ=255)
                                                     ttGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTccc                                    >  1:741759/1‑93 (MQ=255)
                                                         gCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATc                                <  2:4980904/93‑1 (MQ=255)
                                                         gCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGGTAATGCCGGAAACGTGGTTTCCCCATc                                >  2:8933286/1‑93 (MQ=255)
                                                          cacaGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATcc                               <  2:8171010/93‑1 (MQ=255)
                                                          cacaGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATcc                               <  2:4086846/93‑1 (MQ=255)
                                                           acaGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCg                              <  1:4168586/93‑1 (MQ=255)
                                                           acaGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCg                              <  2:9850936/93‑1 (MQ=255)
                                                            caGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCgg                             >  1:9508222/1‑93 (MQ=255)
                                                             aGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGc                            >  2:1508682/1‑93 (MQ=255)
                                                             aGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGc                            >  1:7862888/1‑93 (MQ=255)
                                                             aGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGc                            <  1:7100776/93‑1 (MQ=255)
                                                              gACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCa                           >  2:1099852/1‑93 (MQ=255)
                                                                 gcgTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGCAACCa                        >  2:2188768/1‑93 (MQ=255)
                                                                  cgTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCag                       <  2:7758307/93‑1 (MQ=255)
                                                                    tCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCagag                     <  1:6383034/93‑1 (MQ=255)
                                                                    tCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCagag                     <  2:1002576/93‑1 (MQ=255)
                                                                    tCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCagag                     >  1:2157207/1‑93 (MQ=255)
                                                                      ggCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCa                   >  1:8686970/1‑93 (MQ=255)
                                                                       gCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCCCCAGAGCaa                  <  2:1912953/93‑1 (MQ=255)
                                                                       gCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCaa                  >  1:2364625/1‑93 (MQ=255)
                                                                        cTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCaaa                 >  2:6631565/1‑93 (MQ=255)
                                                                        cTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCaaa                 <  2:6683663/93‑1 (MQ=255)
                                                                         tGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAAt                <  1:1220787/93‑1 (MQ=255)
                                                                          gCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAAtt               <  1:2809561/93‑1 (MQ=255)
                                                                           ccACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTg              >  1:1450858/1‑93 (MQ=255)
                                                                             aCGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCa            <  1:7032260/93‑1 (MQ=255)
                                                                                   cGGCATGCACGCCTTCAGCCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCa      <  1:3469869/93‑1 (MQ=255)
                                                                                    ggCAGGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAg     <  1:1290585/93‑1 (MQ=255)
                                                                                      cATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGAt   <  2:2388891/93‑1 (MQ=255)
                                                                                       aTGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGAtt  <  2:9229960/93‑1 (MQ=255)
                                                                                       aTGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGAtt  <  2:9652992/93‑1 (MQ=255)
                                                                                       aTGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGAtt  >  2:5793497/1‑93 (MQ=255)
                                                                                          |                                                                                         
ATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATT  >  NC_000913/2372556‑2372735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGC  >  NC_000913/2372553‑2372739
                                                                                             |                                                                                             
CTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGC                                                                                               >  SRR4302540.2763964/1‑94 (MQ=60)
CTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGC                                                                                               >  SRR4302540.8262754/1‑94 (MQ=60)
CTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGC                                                                                               >  SRR4302540.2702597/1‑94 (MQ=60)
CTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGC                                                                                               >  SRR4302540.2414641/1‑94 (MQ=60)
 TGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCG                                                                                              <  SRR4302540.8365364/94‑1 (MQ=60)
 TGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCG                                                                                              >  SRR4302540.4742447/1‑94 (MQ=60)
  GATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGC                                                                                             >  SRR4302540.6390906/1‑94 (MQ=60)
  GATAAGTAGGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGC                                                                                             <  SRR4302540.8632326/94‑1 (MQ=60)
     AAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCC                                                                                          <  SRR4302540.6328569/94‑1 (MQ=60)
        TATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTC                                                                                       <  SRR4302540.3906780/94‑1 (MQ=60)
         ATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCA                                                                                      >  SRR4302540.8764905/1‑94 (MQ=60)
         ATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCA                                                                                      >  SRR4302540.214509/1‑94 (MQ=60)
         ATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCA                                                                                      <  SRR4302540.6764710/94‑1 (MQ=60)
          TGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAC                                                                                     >  SRR4302540.3497120/1‑94 (MQ=60)
          TGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAA                                                                                     <  SRR4302540.2733501/94‑1 (MQ=60)
           GGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAAC                                                                                    >  SRR4302540.4800519/1‑94 (MQ=60)
            GACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACC                                                                                   <  SRR4302540.8337763/94‑1 (MQ=60)
             ACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCA                                                                                  <  SRR4302540.3778024/94‑1 (MQ=60)
               AGCGGCTTTCGGCAAACCGGTAGAACCCGCGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCCCGTCGCGGCATGAGCACCTTCACCCAGC                                                                                >  SRR4302540.2030242/1‑94 (MQ=60)
               AGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGC                                                                                >  SRR4302540.8621251/1‑94 (MQ=60)
               AGCGGCTTTCGGCAAACCGGTAGAACCCGAGGCCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGC                                                                                >  SRR4302540.969096/1‑94 (MQ=60)
                 CGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTG                                                                              <  SRR4302540.3395676/94‑1 (MQ=60)
                 CGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTG                                                                              >  SRR4302540.627922/1‑94 (MQ=60)
                 CGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTG                                                                              >  SRR4302540.3157868/1‑94 (MQ=60)
                  GGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGA                                                                             >  SRR4302540.8220292/1‑94 (MQ=60)
                  GGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCCGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGA                                                                             >  SRR4302540.8220293/1‑94 (MQ=60)
                   GCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAA                                                                            <  SRR4302540.6834833/94‑1 (MQ=60)
                    CTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAAT                                                                           <  SRR4302540.9270034/94‑1 (MQ=60)
                    CTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAAT                                                                           <  SRR4302540.8026316/94‑1 (MQ=60)
                     TTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATG                                                                          <  SRR4302540.5764433/94‑1 (MQ=60)
                      TTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGT                                                                         >  SRR4302540.2750526/1‑94 (MQ=60)
                       TCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGATGAATGTG                                                                        >  SRR4302540.9148529/1‑94 (MQ=60)
                        CGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGC                                                                       >  SRR4302540.355717/1‑94 (MQ=60)
                        CGGCAAACCGGAAGAACCCGAGGTCAACGTCACTGAGCACAGACGCGCCGGCTGCCACGGAGCGGCAGGCGCGCCCACAACCAGCTGAAcgggc                                                                       >  SRR4302540.7790848/1‑89 (MQ=55)
                         GGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCA                                                                      <  SRR4302540.4417500/94‑1 (MQ=60)
                         GGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCA                                                                      <  SRR4302540.4699358/94‑1 (MQ=60)
                         GGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCA                                                                      <  SRR4302540.886152/94‑1 (MQ=60)
                         GGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCA                                                                      >  SRR4302540.5016577/1‑94 (MQ=60)
                           CAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGC                                                                    <  SRR4302540.3493184/94‑1 (MQ=60)
                           CAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGC                                                                    <  SRR4302540.3493185/94‑1 (MQ=60)
                            AAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCG                                                                   >  SRR4302540.741207/1‑94 (MQ=60)
                            AAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCG                                                                   <  SRR4302540.5764324/94‑1 (MQ=60)
                            AAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCG                                                                   >  SRR4302540.3392531/1‑94 (MQ=60)
                             AACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGA                                                                  <  SRR4302540.2552953/94‑1 (MQ=60)
                              ACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGAC                                                                 <  SRR4302540.9480889/94‑1 (MQ=60)
                                CGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGT                                                               >  SRR4302540.3965934/1‑94 (MQ=60)
                                 GGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGGGCAGCGACGTT                                                              >  SRR4302540.2728963/1‑94 (MQ=60)
                                  GTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTA                                                             <  SRR4302540.798264/94‑1 (MQ=60)
                                  GTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTA                                                             <  SRR4302540.6602109/94‑1 (MQ=60)
                                    AGAGCCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAAT                                                           <  SRR4302540.2046267/94‑1 (MQ=60)
                                    AGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAAT                                                           <  SRR4302540.3677166/94‑1 (MQ=60)
                                    AGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAAT                                                           <  SRR4302540.6037339/94‑1 (MQ=60)
                                    AGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAAT                                                           <  SRR4302540.8714142/94‑1 (MQ=60)
                                    AGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAAT                                                           <  SRR4302540.8322654/94‑1 (MQ=60)
                                    AGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAAT                                                           <  SRR4302540.7965033/94‑1 (MQ=60)
                                     GAACCCGAGGTCAACGTCATTGAGCGCAGACGCGTCGCCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATG                                                          <  SRR4302540.5775586/94‑1 (MQ=60)
                                     GAACCCGAGGTCAACGTCATTGAGCACAGCCGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGATGTTAATG                                                          <  SRR4302540.5498061/94‑1 (MQ=60)
                                     GAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATG                                                          <  SRR4302540.5498059/94‑1 (MQ=60)
                                     GAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATG                                                          <  SRR4302540.2060241/94‑1 (MQ=60)
                                     GAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATG                                                          >  SRR4302540.3710183/1‑94 (MQ=60)
                                     GAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATG                                                          <  SRR4302540.7313665/94‑1 (MQ=60)
                                     GAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATG                                                          <  SRR4302540.3941659/94‑1 (MQ=60)
                                     GAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATG                                                          <  SRR4302540.297259/94‑1 (MQ=60)
                                       ACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCC                                                        <  SRR4302540.6775097/94‑1 (MQ=60)
                                        CCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCG                                                       <  SRR4302540.10147312/94‑1 (MQ=60)
                                        CCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCG                                                       <  SRR4302540.5059017/94‑1 (MQ=60)
                                         CCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGG                                                      >  SRR4302540.8707689/1‑94 (MQ=60)
                                          CGAGGTCACCGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGA                                                     <  SRR4302540.9438580/94‑1 (MQ=60)
                                               TCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGT                                                <  SRR4302540.9234285/94‑1 (MQ=60)
                                               TCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGG                                                >  SRR4302540.8440086/1‑94 (MQ=60)
                                                CAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTG                                               <  SRR4302540.4895476/94‑1 (MQ=60)
                                                CAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTG                                               <  SRR4302540.754845/94‑1 (MQ=60)
                                                CAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTG                                               <  SRR4302540.7123363/94‑1 (MQ=60)
                                                 AACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGT                                              <  SRR4302540.5550701/94‑1 (MQ=60)
                                                  ACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTT                                             <  SRR4302540.408950/94‑1 (MQ=60)
                                                   CGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTT                                            <  SRR4302540.1704871/94‑1 (MQ=60)
                                                   CGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTT                                            <  SRR4302540.3178852/94‑1 (MQ=60)
                                                    GTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTT                                           >  SRR4302540.1205222/1‑94 (MQ=60)
                                                    GTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTT                                           >  SRR4302540.4995020/1‑94 (MQ=60)
                                                    GTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTT                                           >  SRR4302540.4092333/1‑94 (MQ=60)
                                                    GTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTT                                           >  SRR4302540.1384164/1‑94 (MQ=60)
                                                    GTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTT                                           >  SRR4302540.4942776/1‑94 (MQ=60)
                                                     TCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTC                                          <  SRR4302540.9466684/94‑1 (MQ=60)
                                                       ATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCC                                        >  SRR4302540.749677/1‑94 (MQ=60)
                                                       ATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCC                                        >  SRR4302540.7536222/1‑94 (MQ=60)
                                                       ATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCC                                        >  SRR4302540.1539859/1‑94 (MQ=60)
                                                        TTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCC                                       <  SRR4302540.1834435/94‑1 (MQ=60)
                                                         TGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCA                                      <  SRR4302540.1695682/94‑1 (MQ=60)
                                                         TGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCA                                      <  SRR4302540.8598752/94‑1 (MQ=60)
                                                          GAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCAT                                     <  SRR4302540.4640548/94‑1 (MQ=60)
                                                            GCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCC                                   >  SRR4302540.3831935/1‑94 (MQ=60)
                                                             CACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCG                                  >  SRR4302540.2545403/1‑94 (MQ=60)
                                                              CCAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGG                                 <  SRR4302540.5995872/94‑1 (MQ=60)
                                                              ACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGG                                 <  SRR4302540.4213791/94‑1 (MQ=60)
                                                              ACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGG                                 >  SRR4302540.9620862/1‑94 (MQ=60)
                                                               CAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGC                                >  SRR4302540.7954753/1‑94 (MQ=60)
                                                               CAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGC                                >  SRR4302540.2888753/1‑94 (MQ=60)
                                                                AGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCA                               <  SRR4302540.7179786/94‑1 (MQ=60)
                                                                    GCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAG                           <  SRR4302540.8072412/94‑1 (MQ=60)
                                                                      GTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAG                         <  SRR4302540.2299949/94‑1 (MQ=60)
                                                                      GTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAG                         >  SRR4302540.2180428/1‑94 (MQ=60)
                                                                       TCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGC                        <  SRR4302540.6713231/94‑1 (MQ=60)
                                                                       TCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGC                        <  SRR4302540.6454090/94‑1 (MQ=60)
                                                                        CGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCA                       >  SRR4302540.8787835/1‑94 (MQ=60)
                                                                         GGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAA                      >  SRR4302540.2390090/1‑94 (MQ=60)
                                                                          GCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAA                     <  SRR4302540.8845436/94‑1 (MQ=60)
                                                                           CTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGGAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAAT                    <  SRR4302540.5699404/94‑1 (MQ=60)
                                                                            TGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATT                   <  SRR4302540.1233280/94‑1 (MQ=60)
                                                                             GCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTG                  >  SRR4302540.5988322/1‑94 (MQ=60)
                                                                             GCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTG                  <  SRR4302540.7853233/94‑1 (MQ=60)
                                                                             GCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTG                  >  SRR4302540.1466632/1‑94 (MQ=60)
                                                                             GCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTG                  <  SRR4302540.2839902/94‑1 (MQ=60)
                                                                              CCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGC                 >  SRR4302540.401171/1‑94 (MQ=60)
                                                                               CACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCA                <  SRR4302540.366154/94‑1 (MQ=60)
                                                                               CACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCA                <  SRR4302540.4633939/94‑1 (MQ=60)
                                                                                ACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAG               <  SRR4302540.7110411/94‑1 (MQ=60)
                                                                                ACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAG               >  SRR4302540.4239508/1‑94 (MQ=60)
                                                                                 CGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGC              <  SRR4302540.8524157/94‑1 (MQ=60)
                                                                                 CGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGC              >  SRR4302540.4772517/1‑94 (MQ=60)
                                                                                     GCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCA          >  SRR4302540.1750673/1‑94 (MQ=60)
                                                                                      CGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAG         <  SRR4302540.3981514/94‑1 (MQ=60)
                                                                                      CGGCATGCACGCCTTCAGCCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAG         <  SRR4302540.3507648/94‑1 (MQ=60)
                                                                                       GGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGA        <  SRR4302540.1303924/94‑1 (MQ=60)
                                                                                       GGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGA        <  SRR4302540.1876109/94‑1 (MQ=60)
                                                                                       GGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGA        >  SRR4302540.3339123/1‑94 (MQ=60)
                                                                                       GGCAGGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGA        <  SRR4302540.1303925/94‑1 (MQ=60)
                                                                                         CATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATT      <  SRR4302540.9873217/94‑1 (MQ=60)
                                                                                         CATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATT      <  SRR4302540.2167183/94‑1 (MQ=60)
                                                                                         CATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATT      <  SRR4302540.4860515/94‑1 (MQ=60)
                                                                                          ATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTG     >  SRR4302540.8343404/1‑94 (MQ=60)
                                                                                          ATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTG     >  SRR4302540.2913935/1‑94 (MQ=60)
                                                                                          ATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTG     >  SRR4302540.3320741/1‑94 (MQ=60)
                                                                                          ATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTG     >  SRR4302540.7895684/1‑94 (MQ=60)
                                                                                          ATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTG     >  SRR4302540.1243132/1‑94 (MQ=60)
                                                                                          ATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTG     <  SRR4302540.6835898/94‑1 (MQ=60)
                                                                                            GCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGG   <  SRR4302540.8146364/94‑1 (MQ=60)
                                                                                             CGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGGTTTCCCCATCCGGCACCAGAGGCAATTGCAGCGTCAGATTGGGC  >  SRR4302540.3864604/1‑94 (MQ=60)
                                                                                             |                                                                                             
CTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGC  >  NC_000913/2372553‑2372739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑