Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,372,646 T→C A126A (GCA→GCG)  menE ← o‑succinylbenzoate‑CoA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,372,6460TC100.0% 692.8 / NA 215A126A (GCA→GCG) menEo‑succinylbenzoate‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (111/104);  total (111/104)

Only 100 of 223 total aligned reads displayed.

TAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATT  >  NC_000913/2372557‑2372735
                                                                                         |                                                                                         
tAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGcgcg                                                                                        <  1:153392/93‑1 (MQ=255)
tAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGcgcg                                                                                        >  1:3393870/1‑93 (MQ=255)
   gTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCt                                                                                     >  2:3449404/1‑93 (MQ=255)
    tATGGACAGCGGCTTCCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCtt                                                                                    <  2:2394940/93‑1 (MQ=255)
     aTGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTc                                                                                   >  2:4082015/1‑93 (MQ=255)
        gACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAAc                                                                                <  1:2665592/93‑1 (MQ=255)
        gACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAAc                                                                                <  2:1793572/93‑1 (MQ=255)
         aCAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAAcc                                                                               <  1:3229710/93‑1 (MQ=255)
         aCAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAAcc                                                                               <  1:2229698/93‑1 (MQ=255)
          cAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCa                                                                              >  2:4482853/1‑93 (MQ=255)
           aGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAg                                                                             >  2:3106423/1‑93 (MQ=255)
             cGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCt                                                                           <  1:3001477/93‑1 (MQ=255)
              ggCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTg                                                                          >  1:5248383/1‑93 (MQ=255)
                cTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGaa                                                                        <  2:2699533/93‑1 (MQ=255)
                 tttCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAAt                                                                       <  2:470687/93‑1 (MQ=255)
                  ttCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAAtg                                                                      >  1:476789/1‑93 (MQ=255)
                  ttCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAAtg                                                                      <  2:4087404/93‑1 (MQ=255)
                   tCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAAtgt                                                                     <  2:856438/93‑1 (MQ=255)
                    cGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAAtgtg                                                                    >  1:2876132/1‑93 (MQ=255)
                     ggCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGc                                                                   >  2:1975053/1‑93 (MQ=255)
                      gCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCa                                                                  <  2:3927200/93‑1 (MQ=255)
                       cAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAg                                                                 <  2:497415/93‑1 (MQ=255)
                        aaaCCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGc                                                                <  2:5785524/93‑1 (MQ=255)
                        aaaCCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGc                                                                >  1:1715932/1‑93 (MQ=255)
                         aaCCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCg                                                               <  1:5450871/93‑1 (MQ=255)
                         aaCCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCg                                                               >  2:2275276/1‑93 (MQ=255)
                         aaCCGCTAGAACCCGAGGCCAACGGCATTGAGCACCAACGCGTCACCTGCTACGTAGCAGCATGCGCGCCTTCAACCAGCTTAATGTGCAGCg                                                               >  2:5466983/1‑93 (MQ=255)
                            cGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACg                                                            >  2:1671388/1‑93 (MQ=255)
                             ggTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGt                                                           >  1:3783663/1‑93 (MQ=255)
                             ggTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGt                                                           <  1:1639433/93‑1 (MQ=255)
                             ggTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGt                                                           <  1:2406678/93‑1 (MQ=255)
                              gTAGAACCCGAGGTCAACGTCATTGCGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATATGGAGCGACGtt                                                          >  2:6287217/1‑93 (MQ=255)
                              gTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGACACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGta                                                          >  1:5031941/1‑92 (MQ=255)
                               tAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTa                                                         >  1:578461/1‑93 (MQ=255)
                                aGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTaa                                                        <  2:2263134/93‑1 (MQ=255)
                                aGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTaa                                                        <  2:3217194/93‑1 (MQ=255)
                                aGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTaa                                                        <  1:5615736/93‑1 (MQ=255)
                                aGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCCGAATGTGCAGCGACGTTaa                                                        <  1:2559865/93‑1 (MQ=255)
                                aGAACCCGAGGTCAACGTCATTGACCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTaa                                                        <  2:5858849/93‑1 (MQ=255)
                                  aaCCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATg                                                      <  1:3283626/93‑1 (MQ=255)
                                  aaCCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATg                                                      >  2:4722927/1‑93 (MQ=255)
                                   aCCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGc                                                     <  1:553869/93‑1 (MQ=255)
                                    cccGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGcc                                                    <  1:2384895/93‑1 (MQ=255)
                                    cccGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGcc                                                    <  2:5715523/93‑1 (MQ=255)
                                     ccGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCg                                                   >  2:3652863/1‑93 (MQ=255)
                                      cGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCgg                                                  <  1:4307115/93‑1 (MQ=255)
                                       gAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGa                                                 >  2:2916835/1‑93 (MQ=255)
                                        aGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGaa                                                >  2:5002684/1‑93 (MQ=255)
                                          gTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAAc                                              >  1:4112149/1‑93 (MQ=255)
                                           tCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACg                                             >  2:692503/1‑93 (MQ=255)
                                           tCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACg                                             <  2:4303464/93‑1 (MQ=255)
                                            cAACGTCATTGAGCACAGACGCGTCGGCTGGCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACgt                                            >  1:2103382/1‑93 (MQ=255)
                                            cAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCTTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACgt                                            <  1:3387024/93‑1 (MQ=255)
                                            cAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACgt                                            <  2:297959/93‑1 (MQ=255)
                                             aaCGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACgtg                                           >  1:2837648/1‑93 (MQ=255)
                                             aaCGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACgtg                                           >  2:5397983/1‑93 (MQ=255)
                                             aaCGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACgtg                                           >  1:46941/1‑93 (MQ=255)
                                             aaCGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACgtg                                           >  2:1505643/1‑93 (MQ=255)
                                               cGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGtt                                         <  1:4594703/93‑1 (MQ=255)
                                               cGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGtt                                         <  2:1661470/93‑1 (MQ=255)
                                               cGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGtt                                         <  2:2837814/93‑1 (MQ=255)
                                                 tCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGTCGGAAACGTGtttt                                       >  2:1605900/1‑93 (MQ=255)
                                                 tCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGtttt                                       >  1:1975307/1‑93 (MQ=255)
                                                  cATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTc                                      <  2:4012260/93‑1 (MQ=255)
                                                   aTTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTcc                                     <  1:4386269/93‑1 (MQ=255)
                                                    ttGAGCACAGACGCGTCGGGTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTccc                                    >  2:6166003/1‑93 (MQ=255)
                                                     tGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTcccc                                   <  1:2439811/93‑1 (MQ=255)
                                                      gAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCa                                  <  1:740678/93‑1 (MQ=255)
                                                       aGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCAt                                 <  1:513291/93‑1 (MQ=255)
                                                        gCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATc                                >  2:1028870/1‑93 (MQ=255)
                                                        gCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATc                                >  2:4116629/1‑93 (MQ=255)
                                                        gCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCACCGACGTTAATGCCGGAAACGTGTTTTCCCCATc                                <  2:1919143/93‑1 (MQ=255)
                                                         cacaGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATcc                               <  2:2249942/93‑1 (MQ=255)
                                                          acaGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCg                              >  2:2106120/1‑93 (MQ=255)
                                                            aGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGc                            >  2:4223878/1‑93 (MQ=255)
                                                             gACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCa                           >  1:5994088/1‑93 (MQ=255)
                                                                gcgTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCa                        <  1:1127436/93‑1 (MQ=255)
                                                                gcgTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCa                        <  2:661667/93‑1 (MQ=255)
                                                                    cGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGc                    <  2:5938457/93‑1 (MQ=255)
                                                                     ggCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCa                   >  2:3177725/1‑93 (MQ=255)
                                                                      gCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCaa                  >  1:911878/1‑93 (MQ=255)
                                                                      gCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCaa                  >  2:4562261/1‑93 (MQ=255)
                                                                       cTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCaaa                 >  1:5188871/1‑93 (MQ=255)
                                                                        tGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAAt                >  1:6062034/1‑93 (MQ=255)
                                                                         gCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAAtt               <  1:5112333/93‑1 (MQ=255)
                                                                         gCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAAtt               <  1:5700032/93‑1 (MQ=255)
                                                                            aCGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCa            <  2:1440707/93‑1 (MQ=255)
                                                                             cGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAg           >  2:5118529/1‑93 (MQ=255)
                                                                             cGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAg           >  1:2149685/1‑93 (MQ=255)
                                                                             cGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAg           <  1:514608/93‑1 (MQ=255)
                                                                                 gCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCGAATTGCAGCGTc       >  1:3931811/1‑93 (MQ=255)
                                                                                 gCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTc       >  2:285440/1‑93 (MQ=255)
                                                                                 gCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTc       <  1:2715331/93‑1 (MQ=255)
                                                                                   ggCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAg     <  2:5626662/93‑1 (MQ=255)
                                                                                   ggCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAg     <  1:2209600/93‑1 (MQ=255)
                                                                                   ggCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAg     >  2:3379784/1‑93 (MQ=255)
                                                                                     gaTGCGCCCCTTCAGCCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGAt   <  1:4174261/92‑1 (MQ=255)
                                                                                     cATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGAt   <  1:5399702/93‑1 (MQ=255)
                                                                                      aTGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGAtt  <  2:245248/93‑1 (MQ=255)
                                                                                      aTGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGAtt  <  2:4426115/93‑1 (MQ=255)
                                                                                         |                                                                                         
TAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATT  >  NC_000913/2372557‑2372735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGC  >  NC_000913/2372553‑2372739
                                                                                             |                                                                                             
CTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGC                                                                                               <  SRR4302533.5124538/94‑1 (MQ=60)
 TGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCG                                                                                              >  SRR4302533.1937380/1‑94 (MQ=60)
  GATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGC                                                                                             <  SRR4302533.4985762/94‑1 (MQ=60)
   ATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCG                                                                                            >  SRR4302533.3412872/1‑94 (MQ=60)
   ATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCG                                                                                            >  SRR4302533.3414830/1‑94 (MQ=60)
   ATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCG                                                                                            >  SRR4302533.544703/1‑94 (MQ=60)
    TAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGC                                                                                           <  SRR4302533.154372/94‑1 (MQ=60)
      AGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCT                                                                                         >  SRR4302533.3152470/1‑94 (MQ=60)
         ATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCA                                                                                      >  SRR4302533.5773424/1‑94 (MQ=60)
          TGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAA                                                                                     >  SRR4302533.4678964/1‑94 (MQ=60)
          TGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAA                                                                                     >  SRR4302533.2972495/1‑94 (MQ=60)
            GACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACC                                                                                   <  SRR4302533.2681949/94‑1 (MQ=60)
             ACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCA                                                                                  <  SRR4302533.6058941/94‑1 (MQ=60)
             ACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCA                                                                                  <  SRR4302533.2243460/94‑1 (MQ=60)
             ACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCA                                                                                  <  SRR4302533.3249621/94‑1 (MQ=60)
              CAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAG                                                                                 <  SRR4302533.5613483/94‑1 (MQ=60)
                GCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCT                                                                               >  SRR4302533.2276813/1‑94 (MQ=60)
                 CGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTG                                                                              <  SRR4302533.3019832/94‑1 (MQ=60)
                 CGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTG                                                                              >  SRR4302533.5279932/1‑94 (MQ=60)
                   GCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAA                                                                            >  SRR4302533.1628005/1‑94 (MQ=60)
                    CTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAAT                                                                           <  SRR4302533.2152043/94‑1 (MQ=60)
                    CTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAAT                                                                           >  SRR4302533.2716600/1‑94 (MQ=60)
                     TTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATG                                                                          >  SRR4302533.479635/1‑94 (MQ=60)
                       TCGGCAAGCCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTG                                                                        <  SRR4302533.1523148/94‑1 (MQ=60)
                       TCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTG                                                                        >  SRR4302533.2893832/1‑94 (MQ=60)
                       TCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTG                                                                        >  SRR4302533.2484176/1‑94 (MQ=60)
                           CAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGC                                                                    >  SRR4302533.5624765/1‑94 (MQ=60)
                           CAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGC                                                                    >  SRR4302533.5200559/1‑94 (MQ=60)
                           CAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGC                                                                    >  SRR4302533.1849842/1‑94 (MQ=60)
                           CAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGC                                                                    >  SRR4302533.1726336/1‑94 (MQ=60)
                           CAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGC                                                                    >  SRR4302533.2418953/1‑94 (MQ=60)
                            AAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCG                                                                   >  SRR4302533.5170476/1‑94 (MQ=60)
                             AACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGA                                                                  <  SRR4302533.5483709/94‑1 (MQ=60)
                              ACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGAC                                                                 <  SRR4302533.3640716/94‑1 (MQ=60)
                                CGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGT                                                               >  SRR4302533.2454229/1‑94 (MQ=60)
                                CGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGT                                                               >  SRR4302533.3806831/1‑94 (MQ=60)
                                 GGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTT                                                              <  SRR4302533.2421544/94‑1 (MQ=60)
                                 GGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTT                                                              <  SRR4302533.1649359/94‑1 (MQ=60)
                                 GGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGACACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTA                                                              >  SRR4302533.5062407/1‑94 (MQ=60)
                                  GTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTA                                                             >  SRR4302533.581871/1‑94 (MQ=60)
                                   TANNNNNCNANNTNAANGTNANTGNGCACANANGCGTCGNCTNCCACGTAGCGGCANGNGCGCCTTCAACCNGCTGAATGTGCAGCGACGTTAA                                                            <  SRR4302533.22788/94‑1 (MQ=60)
                                    AGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAAT                                                           <  SRR4302533.5649682/94‑1 (MQ=60)
                                    AGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAAT                                                           <  SRR4302533.5839456/94‑1 (MQ=60)
                                    AGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCCGAATGTGCAGCGACGTTAAT                                                           <  SRR4302533.2575632/94‑1 (MQ=60)
                                     GAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATG                                                          <  SRR4302533.4776430/94‑1 (MQ=60)
                                      AACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGC                                                         <  SRR4302533.3303896/94‑1 (MQ=60)
                                       ACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCC                                                        <  SRR4302533.557153/94‑1 (MQ=60)
                                       ACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCC                                                        <  SRR4302533.6096154/94‑1 (MQ=60)
                                        CCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCG                                                       >  SRR4302533.2034791/1‑94 (MQ=60)
                                        CCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCG                                                       >  SRR4302533.2792846/1‑94 (MQ=60)
                                        CCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCG                                                       <  SRR4302533.2399634/94‑1 (MQ=60)
                                          CGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGGCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGA                                                     >  SRR4302533.807521/1‑94 (MQ=60)
                                          CGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGA                                                     <  SRR4302533.4333037/94‑1 (MQ=60)
                                           GAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAA                                                    >  SRR4302533.3297185/1‑94 (MQ=60)
                                            AGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAA                                                   >  SRR4302533.2191604/1‑94 (MQ=60)
                                             GGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAAC                                                  >  SRR4302533.4137143/1‑94 (MQ=60)
                                               TCAACGTCATTGAGCACAGACGCGTCGGCTGGCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGT                                                >  SRR4302533.2116349/1‑94 (MQ=60)
                                                CAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCTTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTG                                               <  SRR4302533.3407936/94‑1 (MQ=60)
                                                CAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTG                                               >  SRR4302533.47265/1‑94 (MQ=60)
                                                CAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTG                                               <  SRR4302533.2635226/94‑1 (MQ=60)
                                                CAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTG                                               >  SRR4302533.2855113/1‑94 (MQ=60)
                                                CAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTG                                               >  SRR4302533.5544218/1‑94 (MQ=60)
                                                  ACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTT                                             >  SRR4302533.4356366/1‑94 (MQ=60)
                                                   CGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTT                                            <  SRR4302533.338836/94‑1 (MQ=60)
                                                   CGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTT                                            <  SRR4302533.4622537/94‑1 (MQ=60)
                                                    GTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTT                                           >  SRR4302533.1987404/1‑94 (MQ=60)
                                                     TCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTC                                          >  SRR4302533.3852031/1‑94 (MQ=60)
                                                     TCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTC                                          >  SRR4302533.5680149/1‑94 (MQ=60)
                                                      CATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCC                                         >  SRR4302533.4331929/1‑94 (MQ=60)
                                                      CATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCC                                         >  SRR4302533.694707/1‑94 (MQ=60)
                                                       ATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCC                                        <  SRR4302533.4412752/94‑1 (MQ=60)
                                                       ATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCC                                        <  SRR4302533.3192408/94‑1 (MQ=60)
                                                        TTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCC                                       <  SRR4302533.2012607/94‑1 (MQ=60)
                                                         TGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCA                                      <  SRR4302533.2454879/94‑1 (MQ=60)
                                                          GAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCAT                                     <  SRR4302533.745035/94‑1 (MQ=60)
                                                           AGCACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATC                                    <  SRR4302533.516342/94‑1 (MQ=60)
                                                             CACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCG                                  <  SRR4302533.1696123/94‑1 (MQ=60)
                                                             CACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCG                                  >  SRR4302533.2393665/1‑94 (MQ=60)
                                                              ACAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGG                                 <  SRR4302533.4182753/94‑1 (MQ=60)
                                                               CAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGC                                >  SRR4302533.4468848/1‑94 (MQ=60)
                                                               CAGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGC                                >  SRR4302533.4339209/1‑94 (MQ=60)
                                                                AGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCA                               >  SRR4302533.6030392/1‑94 (MQ=60)
                                                                AGACGCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCA                               >  SRR4302533.367050/1‑94 (MQ=60)
                                                                    GCGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAG                           <  SRR4302533.1134134/94‑1 (MQ=60)
                                                                     CGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCTGCGACGTTAATGCAGGAAACGTGTTTTCCCCATCCGGCACCAGA                          >  SRR4302533.514664/1‑94 (MQ=60)
                                                                     CGTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGA                          >  SRR4302533.4058236/1‑94 (MQ=60)
                                                                      GTCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAG                         <  SRR4302533.4981227/94‑1 (MQ=60)
                                                                       TCGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGC                        <  SRR4302533.5141685/94‑1 (MQ=60)
                                                                        CGGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCA                       <  SRR4302533.2712583/94‑1 (MQ=60)
                                                                         GGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAA                      >  SRR4302533.917226/1‑94 (MQ=60)
                                                                         GGCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAA                      >  SRR4302533.4907973/1‑94 (MQ=60)
                                                                          GCTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAA                     >  SRR4302533.5220185/1‑94 (MQ=60)
                                                                           CTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAAT                    >  SRR4302533.6098731/1‑94 (MQ=60)
                                                                           CTGCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAAT                    >  SRR4302533.2867642/1‑94 (MQ=60)
                                                                             GCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTG                  <  SRR4302533.5206808/94‑1 (MQ=60)
                                                                             GCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTG                  <  SRR4302533.5734453/94‑1 (MQ=60)
                                                                             GCCACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTG                  <  SRR4302533.5143226/94‑1 (MQ=60)
                                                                               CACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCA                >  SRR4302533.5236269/1‑94 (MQ=60)
                                                                               CACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCA                <  SRR4302533.2227793/94‑1 (MQ=60)
                                                                                ACGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAG               >  SRR4302533.2162921/1‑94 (MQ=60)
                                                                                 CGTAGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGC              <  SRR4302533.517665/94‑1 (MQ=60)
                                                                                    AGCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCGAATTGCAGCGTC           >  SRR4302533.3955815/1‑94 (MQ=60)
                                                                                     GCGGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCA          <  SRR4302533.2732002/94‑1 (MQ=60)
                                                                                       GGCATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGA        <  SRR4302533.2223227/94‑1 (MQ=60)
                                                                                         CATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATT      <  SRR4302533.5432148/94‑1 (MQ=60)
                                                                                         CATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATT      <  SRR4302533.5351250/94‑1 (MQ=60)
                                                                                          ATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTG     >  SRR4302533.493742/1‑94 (MQ=60)
                                                                                          ATGCGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTG     >  SRR4302533.658277/1‑94 (MQ=60)
                                                                                             CGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGC  >  SRR4302533.6185115/1‑94 (MQ=60)
                                                                                             CGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGC  >  SRR4302533.2009308/1‑94 (MQ=60)
                                                                                             CGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGC  >  SRR4302533.1310414/1‑94 (MQ=60)
                                                                                             |                                                                                             
CTGATAAGTATGGACAGCGGCTTTCGGCAAACCGGTAGAACCCGAGGTCAACGTCATTGAGCACAGACGCGTCGGCTGCCACGTAGCGGCATGTGCGCCTTCAACCAGCTGAATGTGCAGCGACGTTAATGCCGGAAACGTGTTTTCCCCATCCGGCACCAGAGCAAATTGCAGCGTCAGATTGGGC  >  NC_000913/2372553‑2372739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑