Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,393,734 A→G G61G (GGT→GGC)  nuoJ ← NADH dehydrogenase I chain J

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,393,7340AG99.6% 773.1 / ‑4.9 243G61G (GGT→GGC) nuoJNADH dehydrogenase I chain J
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (126/116);  total (126/117)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.49e-01

Only 100 of 253 total aligned reads displayed.

CTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCACCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAAATAATCAGGTA  >  NC_000913/2393644‑2393826
                                                                                          |                                                                                            
cTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGcc                                                                                            >  1:939434/1‑93 (MQ=255)
    ggTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCcgcg                                                                                        >  1:7306817/1‑93 (MQ=255)
     gTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGCTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGt                                                                                       >  1:1566280/1‑93 (MQ=255)
      tttCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTa                                                                                      <  2:1762970/93‑1 (MQ=255)
      tttCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTa                                                                                      <  2:9221454/93‑1 (MQ=255)
      tttCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTa                                                                                      >  1:4793897/1‑93 (MQ=255)
       ttCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAg                                                                                     <  1:5620871/93‑1 (MQ=255)
       ttCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAg                                                                                     <  1:5277218/93‑1 (MQ=255)
        tCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGa                                                                                    <  1:1004542/93‑1 (MQ=255)
        tCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGa                                                                                    <  1:4119666/93‑1 (MQ=255)
          aGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACg                                                                                  <  2:7165731/93‑1 (MQ=255)
           gCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGa                                                                                 >  1:9295375/1‑93 (MQ=255)
           gCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGa                                                                                 <  2:8252944/93‑1 (MQ=255)
            ccACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGAt                                                                                <  1:8513391/93‑1 (MQ=255)
             cACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATa                                                                               <  2:2663305/93‑1 (MQ=255)
              aCTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATaa                                                                              >  1:140821/1‑93 (MQ=255)
               cTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGACCAGCACCATAATGGCGCCCGCGTAGACGATAAt                                                                             >  1:9720384/1‑93 (MQ=255)
               cTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAAt                                                                             <  2:6271595/93‑1 (MQ=255)
               cTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAAt                                                                             <  2:9538124/93‑1 (MQ=255)
                tGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAAtt                                                                            <  1:974223/93‑1 (MQ=255)
                tGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAAtt                                                                            >  2:5791874/1‑93 (MQ=255)
                 ggCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAAttt                                                                           <  2:4839575/93‑1 (MQ=255)
                  gCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTc                                                                          >  1:2015095/1‑93 (MQ=255)
                  gCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTc                                                                          >  1:6105637/1‑93 (MQ=255)
                  gCGTTCCTGTTCGATTTCTGAACCGCCCAGGCTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGAAGACGATAATTTc                                                                          >  1:9407500/1‑93 (MQ=255)
                   cGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTcc                                                                         <  1:8468612/93‑1 (MQ=255)
                     ttCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAg                                                                       >  2:6855183/1‑93 (MQ=255)
                     ttCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAg                                                                       >  2:4073210/1‑93 (MQ=255)
                     ttCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAg                                                                       <  1:6228865/93‑1 (MQ=255)
                     ttCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAg                                                                       >  1:6012014/1‑93 (MQ=255)
                     ttCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGCAGACGATAATTTCCAg                                                                       >  2:1059589/1‑93 (MQ=255)
                      tCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAgc                                                                      >  1:801274/1‑93 (MQ=255)
                        cTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAgcgc                                                                    >  2:7481536/1‑93 (MQ=255)
                         tGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCa                                                                   >  2:8052582/1‑93 (MQ=255)
                          gTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCAc                                                                  >  1:8095739/1‑93 (MQ=255)
                          gTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCAc                                                                  >  2:8072948/1‑93 (MQ=255)
                          gTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCAc                                                                  <  2:7754765/93‑1 (MQ=255)
                           ttCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCAcg                                                                 >  1:4560351/1‑92 (MQ=255)
                           ttCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCAcc                                                                 <  1:3318284/93‑1 (MQ=255)
                             cGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCgg                                                               <  2:4218906/93‑1 (MQ=255)
                              gATTTCTGAACCGCCCAGGTTGAGCGTCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGc                                                              >  1:2735698/1‑93 (MQ=255)
                              gATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGc                                                              >  2:2156605/1‑93 (MQ=255)
                              gATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGc                                                              >  2:3033700/1‑93 (MQ=255)
                                tttCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGa                                                            <  1:7067158/93‑1 (MQ=255)
                                   cTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGt                                                         >  1:9568256/1‑93 (MQ=255)
                                    tGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTa                                                        <  2:3553493/93‑1 (MQ=255)
                                    tGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTa                                                        <  2:4168171/93‑1 (MQ=255)
                                     gAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTaa                                                       >  2:210282/1‑93 (MQ=255)
                                     gAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTaa                                                       >  1:2279138/1‑93 (MQ=255)
                                     gAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGAACCATAATGGCGCCCGCGTAACCGATAATTTCCAGCCCCCGGGCGGAGTaa                                                       >  1:5761572/1‑93 (MQ=255)
                                      aaCCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTacg                                                      <  2:7026882/93‑3 (MQ=255)
                                      aaCCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAg                                                      <  2:1404666/93‑1 (MQ=255)
                                      aaCCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAg                                                      <  1:9202079/93‑1 (MQ=255)
                                          gCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGcc                                                  >  2:3342894/1‑93 (MQ=255)
                                           cccAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGccc                                                 <  2:930552/93‑1 (MQ=255)
                                           cccAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGccc                                                 <  1:1683880/93‑1 (MQ=255)
                                            ccAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCa                                                >  2:4480316/1‑93 (MQ=255)
                                            ccAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCa                                                <  2:2396315/93‑1 (MQ=255)
                                             cAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAg                                               <  1:9596322/93‑1 (MQ=255)
                                              aGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAgg                                              >  2:1871160/1‑92 (MQ=255)
                                              aGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGt                                              <  2:5988864/93‑1 (MQ=255)
                                               ggTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTg                                             >  2:2910555/1‑93 (MQ=255)
                                                gTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTga                                            <  2:7445120/93‑1 (MQ=255)
                                                  tGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTgaga                                          <  2:2554142/93‑1 (MQ=255)
                                                    aGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGagaag                                        <  2:1551276/93‑1 (MQ=255)
                                                     gCATCATCACCACGAACTCGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGagaaga                                       >  2:8960077/1‑93 (MQ=255)
                                                     gCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCAACGGCGAACAAAACGCCCAGTCagaaga                                       >  1:4818719/1‑93 (MQ=255)
                                                      catcatCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAgaagaa                                      <  2:5432288/93‑1 (MQ=255)
                                                       atcatcACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAac                                     <  2:6544151/93‑1 (MQ=255)
                                                       atcatcACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAac                                     >  2:5030664/1‑93 (MQ=255)
                                                        tcatcaCCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAgaagaaac                                    >  1:4415058/1‑91 (MQ=255)
                                                        tcatcaCCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAaca                                    <  2:7412036/93‑1 (MQ=255)
                                                        tcatcaCCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAaca                                    >  2:6803629/1‑93 (MQ=255)
                                                        tcatcaCCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAaca                                    >  2:1642427/1‑93 (MQ=255)
                                                        tcatcaCCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAaca                                    >  1:2989205/1‑93 (MQ=255)
                                                         catcaCCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAacac                                   <  1:7621216/93‑1 (MQ=255)
                                                         catcaCCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGAAAGCGCCCAGTGAGAAGAacac                                   >  1:6274705/1‑93 (MQ=255)
                                                           tcaCCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACAccc                                 >  1:7716913/1‑93 (MQ=255)
                                                            caccacGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACAcccc                                <  2:643725/93‑1 (MQ=255)
                                                             accacGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCg                               >  2:7046435/1‑93 (MQ=255)
                                                                acgaacacgaacAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAACGAAGCGCCCAGTGAGAAGAACACCCCGCaa                            >  1:2469939/1‑93 (MQ=255)
                                                                 cgaacacgaacaGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGaaa                           <  1:4318423/93‑1 (MQ=255)
                                                                  gaacacgaacaGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAAt                          >  1:9843982/1‑93 (MQ=255)
                                                                  gaacacgaacaGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAAt                          >  1:5805441/1‑93 (MQ=255)
                                                                   aacacgaacaGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATc                         >  2:891365/1‑93 (MQ=255)
                                                                    acacgaacaGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAAtag                        >  2:3651178/1‑91 (MQ=255)
                                                                    acacgaacaGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCg                        >  1:8245769/1‑93 (MQ=255)
                                                                    acacgaacaGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCg                        >  2:5553584/1‑93 (MQ=255)
                                                                    acacgaacaGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCg                        >  2:3922539/1‑93 (MQ=255)
                                                                      acgaacaGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGcc                      <  1:5325718/93‑1 (MQ=255)
                                                                       cgaacaGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCca                     >  1:8714717/1‑93 (MQ=255)
                                                                       cgaacaGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCca                     >  2:1985748/1‑93 (MQ=255)
                                                                       cgaacaGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCca                     <  2:9717150/93‑1 (MQ=255)
                                                                           caGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCcagcag                 <  1:5574930/93‑1 (MQ=255)
                                                                            aGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACTGGCGAAGTAAGCGACCAGTGAGAAGAACACCCCGGAAATCGCCagcagc                >  1:7920882/1‑93 (MQ=255)
                                                                               aCCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGaa             >  2:6618124/1‑93 (MQ=255)
                                                                               aCCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGaa             >  2:1195829/1‑93 (MQ=255)
                                                                                 cATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAaat           <  1:1908399/93‑1 (MQ=255)
                                                                                   tAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAaataa         >  2:9841533/1‑93 (MQ=255)
                                                                                    aaTGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAaataat        <  2:7550461/93‑1 (MQ=255)
                                                                                          |                                                                                            
CTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCACCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAAATAATCAGGTA  >  NC_000913/2393644‑2393826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCACCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAAATAATCAGGTA  >  NC_000913/2393641‑2393826
                                                                                             |                                                                                            
CACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCG                                                                                              <  SRR4302540.3261656/94‑1 (MQ=60)
 ACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGC                                                                                             >  SRR4302540.2912490/1‑94 (MQ=60)
  CCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCC                                                                                            >  SRR4302540.7967135/1‑94 (MQ=60)
  CCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCC                                                                                            >  SRR4302540.949262/1‑94 (MQ=60)
   CTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCC                                                                                           >  SRR4302540.2840531/1‑94 (MQ=60)
   CTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCC                                                                                           <  SRR4302540.7496360/94‑1 (MQ=60)
   CTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCC                                                                                           >  SRR4302540.7616040/1‑94 (MQ=60)
     GCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGC                                                                                         <  SRR4302540.8593995/94‑1 (MQ=60)
      CGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCG                                                                                        >  SRR4302540.7388390/1‑94 (MQ=60)
      CGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCG                                                                                        >  SRR4302540.5681115/1‑94 (MQ=60)
      CGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCACCCGCG                                                                                        <  SRR4302540.5826633/94‑1 (MQ=60)
       GGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGCTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGT                                                                                       >  SRR4302540.1583464/1‑94 (MQ=60)
        GTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTA                                                                                      >  SRR4302540.4845178/1‑94 (MQ=60)
         TTTCAGCCACTGGCGTTCCTGTGCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAG                                                                                     <  SRR4302540.8232414/94‑1 (MQ=60)
          TTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGA                                                                                    <  SRR4302540.5682713/94‑1 (MQ=60)
          TTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGA                                                                                    <  SRR4302540.5334838/94‑1 (MQ=60)
          TTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGA                                                                                    <  SRR4302540.3218452/94‑1 (MQ=60)
           TCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGAC                                                                                   <  SRR4302540.1985881/94‑1 (MQ=60)
           TCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGAC                                                                                   <  SRR4302540.1014735/94‑1 (MQ=60)
           TCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGAC                                                                                   <  SRR4302540.4164147/94‑1 (MQ=60)
            CAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACG                                                                                  >  SRR4302540.9944257/1‑94 (MQ=60)
             nnnccCTNGNGTTCCTGTTCNNNNTNTGAACCGCCCAGGTTCAGCATCATCACCACGAACACGAACAGCCCCATAATGGCGCCCGCGTAGACGA                                                                                 <  SRR4302540.2518575/89‑1 (MQ=60)
             AGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGA                                                                                 >  SRR4302540.9406430/1‑94 (MQ=60)
               CCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATA                                                                               <  SRR4302540.8612328/94‑1 (MQ=60)
                CACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAA                                                                              <  SRR4302540.117281/94‑1 (MQ=60)
                CACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAA                                                                              >  SRR4302540.142288/1‑94 (MQ=60)
                 ACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGACCAGCACCATAATGGCGCCCGCGTAGACGATAAT                                                                             >  SRR4302540.9834578/1‑94 (MQ=60)
                 ACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAAT                                                                             >  SRR4302540.9834761/1‑94 (MQ=60)
                  CTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATT                                                                            >  SRR4302540.5209356/1‑94 (MQ=60)
                   TGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTT                                                                           >  SRR4302540.8979837/1‑94 (MQ=60)
                   TGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTT                                                                           <  SRR4302540.984229/94‑1 (MQ=60)
                    GGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTC                                                                          >  SRR4302540.2036878/1‑94 (MQ=60)
                    GGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTC                                                                          >  SRR4302540.6173634/1‑94 (MQ=60)
                    GGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTC                                                                          >  SRR4302540.9519377/1‑94 (MQ=60)
                    GGCGTTCCTGTTCGATTTCTGAACCGCCCAGGCTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGAAGACGATAATTTC                                                                          >  SRR4302540.9519378/1‑94 (MQ=60)
                     GCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCC                                                                         <  SRR4302540.2129517/94‑1 (MQ=60)
                      CGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCA                                                                        <  SRR4302540.8566948/94‑1 (MQ=60)
                      CGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCA                                                                        >  SRR4302540.832590/1‑94 (MQ=60)
                       GTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAG                                                                       >  SRR4302540.6078683/1‑94 (MQ=60)
                        TTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATACTGGCGCCCGCGTAGACGATAATTTCCAGC                                                                      >  SRR4302540.247534/1‑94 (MQ=60)
                        TTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGC                                                                      >  SRR4302540.809783/1‑94 (MQ=60)
                        TTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGC                                                                      <  SRR4302540.6298138/94‑1 (MQ=60)
                           CTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCA                                                                   >  SRR4302540.4353063/1‑94 (MQ=60)
                           CTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCA                                                                   >  SRR4302540.9714475/1‑94 (MQ=60)
                            TGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCAC                                                                  >  SRR4302540.8189706/1‑94 (MQ=60)
                             GTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACG                                                                 >  SRR4302540.4609628/1‑94 (MQ=60)
                             GTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACC                                                                 <  SRR4302540.8666020/94‑1 (MQ=60)
                             GTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACC                                                                 <  SRR4302540.9926403/94‑1 (MQ=60)
                              TTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCG                                                                >  SRR4302540.8435401/1‑94 (MQ=60)
                              TTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCG                                                                <  SRR4302540.3354674/94‑1 (MQ=60)
                               TCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGG                                                               >  SRR4302540.9550452/1‑94 (MQ=60)
                                CGATTTCTGAACCGCCCAGGTTGAGCGTCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGC                                                              >  SRR4302540.2765228/1‑94 (MQ=60)
                                   TTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAA                                                           <  SRR4302540.4764018/94‑1 (MQ=60)
                                   TTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAA                                                           <  SRR4302540.7145760/94‑1 (MQ=60)
                                    TTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAG                                                          <  SRR4302540.6213818/94‑1 (MQ=60)
                                     TCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGT                                                         >  SRR4302540.9681321/1‑94 (MQ=60)
                                       TGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAA                                                       >  SRR4302540.2303621/1‑94 (MQ=60)
                                       TGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGAACCATAATGGCGCCCGCGTAACCGATAATTTCCAGCccccgggcggagtaa                                                       >  SRR4302540.5825186/1‑79 (MQ=60)
                                        GAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAG                                                      >  SRR4302540.2194696/1‑94 (MQ=60)
                                         AACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGC                                                     <  SRR4302540.4858689/94‑1 (MQ=60)
                                         AACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGC                                                     <  SRR4302540.9312490/94‑1 (MQ=60)
                                             GCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCC                                                 >  SRR4302540.5198621/1‑94 (MQ=60)
                                              CCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCA                                                >  SRR4302540.8597564/1‑94 (MQ=60)
                                              CCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCA                                                <  SRR4302540.1702151/94‑1 (MQ=60)
                                              CCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCA                                                <  SRR4302540.1702152/94‑1 (MQ=60)
                                               CCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAG                                               >  SRR4302540.6044280/1‑94 (MQ=60)
                                                CAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCGGCGTAGACGATAATTTCGAGCGCACCGGCGAAGTAcggccccagt                                              >  SRR4302540.4577293/1‑84 (MQ=60)
                                                CAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGT                                              <  SRR4302540.9709556/94‑1 (MQ=60)
                                                  GGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGA                                            >  SRR4302540.6161769/1‑94 (MQ=60)
                                                   GTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAG                                           <  SRR4302540.5361339/94‑1 (MQ=60)
                                                   GTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGAAAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAG                                           <  SRR4302540.5792033/94‑1 (MQ=60)
                                                     TGAGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAA                                         <  SRR4302540.2698165/94‑1 (MQ=60)
                                                       AGCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCAACGGCGAACAAAACGCCCAGTCAGAAGA                                       >  SRR4302540.4870300/1‑94 (MQ=60)
                                                        GCATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAA                                      <  SRR4302540.969893/94‑1 (MQ=60)
                                                         CATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAAC                                     <  SRR4302540.5887881/94‑1 (MQ=60)
                                                         CATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAAC                                     <  SRR4302540.3181809/94‑1 (MQ=60)
                                                         CATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAAC                                     >  SRR4302540.5497198/1‑94 (MQ=60)
                                                          ATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAAac                                    >  SRR4302540.4462905/1‑92 (MQ=60)
                                                          ATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACA                                    >  SRR4302540.3022169/1‑94 (MQ=60)
                                                          ATCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCAAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACA                                    >  SRR4302540.704021/1‑94 (MQ=60)
                                                           TCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACAC                                   <  SRR4302540.8951149/94‑1 (MQ=60)
                                                           TCATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGAAAGCGCCCAGTGAGAAGAACAC                                   >  SRR4302540.6344511/1‑94 (MQ=60)
                                                            CATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACC                                  <  SRR4302540.7706918/94‑1 (MQ=60)
                                                             ATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCC                                 >  SRR4302540.7806075/1‑94 (MQ=60)
                                                             ATCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCC                                 >  SRR4302540.7806076/1‑94 (MQ=60)
                                                              TCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCC                                <  SRR4302540.7354583/94‑1 (MQ=60)
                                                              TCACCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCC                                >  SRR4302540.9654709/1‑94 (MQ=60)
                                                                 CCACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCCGA                             >  SRR4302540.541508/1‑94 (MQ=60)
                                                                  CACGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAACGAAGCGCCCAGTGAGAAGAACACCCCGCAA                            >  SRR4302540.2496528/1‑94 (MQ=60)
                                                                    CGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAAT                          >  SRR4302540.9959057/1‑94 (MQ=60)
                                                                    CGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAAT                          <  SRR4302540.4365432/94‑1 (MQ=60)
                                                                    CGAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAAT                          >  SRR4302540.5869592/1‑94 (MQ=60)
                                                                     GAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATC                         >  SRR4302540.1462796/1‑94 (MQ=60)
                                                                     GAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATC                         <  SRR4302540.939631/94‑1 (MQ=60)
                                                                     GAACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATC                         <  SRR4302540.9375978/94‑1 (MQ=60)
                                                                      AACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCG                        >  SRR4302540.8341608/1‑94 (MQ=60)
                                                                      AACACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCG                        <  SRR4302540.7873552/94‑1 (MQ=60)
                                                                        CACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCC                      >  SRR4302540.4428109/1‑94 (MQ=60)
                                                                         ACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCA                     >  SRR4302540.8815887/1‑94 (MQ=60)
                                                                         ACGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCA                     <  SRR4302540.5384051/94‑1 (MQ=60)
                                                                          CGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAACTCGCCAC                    >  SRR4302540.1795532/1‑94 (MQ=60)
                                                                          CGAACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAG                    >  SRR4302540.1795531/1‑94 (MQ=60)
                                                                            AACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCA                  <  SRR4302540.8114366/94‑1 (MQ=60)
                                                                             ACAGCCCCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAG                 <  SRR4302540.3461824/94‑1 (MQ=60)
                                                                             ACAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAG                 >  SRR4302540.814275/1‑94 (MQ=60)
                                                                              CAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACTGGCGAAGTAAGCGACCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGC                >  SRR4302540.8013199/1‑94 (MQ=60)
                                                                              CAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGC                >  SRR4302540.4226770/1‑94 (MQ=60)
                                                                              CAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGC                >  SRR4302540.6475557/1‑94 (MQ=60)
                                                                              CAGCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGC                <  SRR4302540.5636306/94‑1 (MQ=60)
                                                                                GCACCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGA              >  SRR4302540.9708143/1‑94 (MQ=60)
                                                                                   CCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAAAT           >  SRR4302540.4563472/1‑94 (MQ=60)
                                                                                   CCATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAAAA           >  SRR4302540.1496045/1‑94 (MQ=60)
                                                                                    CATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAAATA          <  SRR4302540.1929250/94‑1 (MQ=60)
                                                                                     ATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAAATAA         >  SRR4302540.9454430/1‑94 (MQ=60)
                                                                                     ATAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAAATAA         <  SRR4302540.4856099/94‑1 (MQ=60)
                                                                                      TAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAAATAAT        <  SRR4302540.5718873/94‑1 (MQ=60)
                                                                                      TAATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAAATAAT        <  SRR4302540.8014149/94‑1 (MQ=60)
                                                                                        ATGGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAAATAATCA      >  SRR4302540.6183428/1‑94 (MQ=60)
                                                                                          GGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAAATAATCAGG    <  SRR4302540.6337912/94‑1 (MQ=60)
                                                                                          GGCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAAATAATCAGG    <  SRR4302540.2705419/94‑1 (MQ=60)
                                                                                           GCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAAATAATCAGGT   <  SRR4302540.2827721/94‑1 (MQ=60)
                                                                                           GCGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAAATAATCAGGT   >  SRR4302540.150138/1‑94 (MQ=60)
                                                                                            CGCCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAAATAATCAGGTA  <  SRR4302540.7564346/94‑1 (MQ=60)
                                                                                             |                                                                                            
CACCTGCGGTTTCAGCCACTGGCGTTCCTGTTCGATTTCTGAACCGCCCAGGTTGAGCATCATCACCACGAACACGAACAGCACCATAATGGCACCCGCGTAGACGATAATTTCCAGCGCACCGGCGAAGTAAGCGCCCAGTGAGAAGAACACCCCGGAAATCGCCAGCAGCGAAATAATCAGGTA  >  NC_000913/2393641‑2393826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑