Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,473,032 A→G K19R (AAG→AGG)  b2361 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,473,0320AG91.4% 1055.6 / 69.7 395K19R (AAG→AGG) b2361hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (15/19);  new base G (188/173);  total (203/192)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

Only 100 of 398 total aligned reads displayed.

ATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTC  >  NC_000913/2472942‑2473121
                                                                                          |                                                                                         
 tGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGct                                                                                        >  2:2922661/1‑92 (MQ=255)
 tGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCa                                                                                        >  1:6249449/1‑93 (MQ=255)
 tGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCa                                                                                        >  1:8160169/1‑93 (MQ=255)
 tGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCa                                                                                        <  2:7723531/93‑1 (MQ=255)
  ggTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCAt                                                                                       <  2:839611/93‑1 (MQ=255)
  ggTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGAGAGGCAt                                                                                       >  1:9317358/1‑93 (MQ=255)
   gTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCAtt                                                                                      <  1:5036052/93‑1 (MQ=255)
    tGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCAttt                                                                                     <  1:1334606/93‑1 (MQ=255)
     gAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCAtttt                                                                                    <  1:5232354/93‑1 (MQ=255)
     gAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCAtttt                                                                                    >  1:7775947/1‑93 (MQ=255)
       gggCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTtta                                                                                  >  2:118891/1‑93 (MQ=255)
         gCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTttatt                                                                                >  1:2991153/1‑93 (MQ=255)
          cTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTttatta                                                                               <  2:3480485/93‑1 (MQ=255)
          cTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTttatta                                                                               <  2:191797/93‑1 (MQ=255)
          cTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTttatta                                                                               <  2:6165509/93‑1 (MQ=255)
           ttCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACATAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTtattat                                                                              >  2:4467818/1‑93 (MQ=255)
           ttCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTtattat                                                                              <  2:7018168/93‑1 (MQ=255)
           ttCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTtattat                                                                              <  1:3564395/93‑1 (MQ=255)
           ttCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTtattat                                                                              <  1:1483361/93‑1 (MQ=255)
            tCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATg                                                                             >  2:2453935/1‑93 (MQ=255)
            tCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATg                                                                             >  2:7602887/1‑93 (MQ=255)
            tCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATg                                                                             <  1:6887602/93‑1 (MQ=255)
            tCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATg                                                                             >  2:7387844/1‑93 (MQ=255)
            tCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATg                                                                             >  2:6840243/1‑93 (MQ=255)
             ccTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGa                                                                            <  1:6651599/93‑1 (MQ=255)
             ccTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGa                                                                            >  1:978885/1‑93 (MQ=255)
             ccTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGa                                                                            >  1:3724045/1‑93 (MQ=255)
              cTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGAc                                                                           >  2:6416439/1‑93 (MQ=255)
              cTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGAc                                                                           >  2:8591440/1‑93 (MQ=255)
              cTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGAc                                                                           >  2:3150169/1‑93 (MQ=255)
               ttttACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACa                                                                          >  1:3158280/1‑93 (MQ=255)
               ttttACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACa                                                                          >  1:8328784/1‑93 (MQ=255)
                tttACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACaa                                                                         <  1:792583/93‑1 (MQ=255)
                tttACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACaa                                                                         <  1:4066682/93‑1 (MQ=255)
                 ttACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAg                                                                        <  1:9051290/93‑1 (MQ=255)
                  tACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGa                                                                       <  2:3015025/93‑1 (MQ=255)
                     ccAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGataa                                                                    >  1:7578064/1‑93 (MQ=255)
                      cAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGataaa                                                                   <  1:4428029/93‑1 (MQ=255)
                      cAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGataaa                                                                   <  2:9224487/93‑1 (MQ=255)
                      cAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGataaa                                                                   <  2:345520/93‑1 (MQ=255)
                       aaaCTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATAACAAGataaat                                                                  <  1:7393860/93‑1 (MQ=255)
                        aaCTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGataaata                                                                 <  2:2073170/93‑1 (MQ=255)
                        aaCTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGataaata                                                                 <  1:437067/93‑1 (MQ=255)
                        aaCTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGataaata                                                                 <  2:4266800/93‑1 (MQ=255)
                        aaCTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGataaata                                                                 <  2:5039994/93‑1 (MQ=255)
                         aCTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGataaataa                                                                <  2:4073577/93‑1 (MQ=255)
                           tCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAg                                                              <  2:324903/93‑1 (MQ=255)
                            cAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAga                                                             <  2:2677348/93‑1 (MQ=255)
                            cAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAga                                                             <  2:5429321/93‑1 (MQ=255)
                               cgcgGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAgacga                                                          <  1:9638119/93‑1 (MQ=255)
                               cgcgGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAgacga                                                          >  2:5644185/1‑93 (MQ=255)
                                gcgGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAgacgac                                                         <  1:2804279/93‑1 (MQ=255)
                                 cgGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACa                                                        >  1:5896948/1‑93 (MQ=255)
                                  gtggCAGGGANNATNCGGGGAGAACAAAATGTNATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGANAt                                                       <  2:4706729/90‑1 (MQ=255)
                                   gTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATc                                                      >  2:62065/1‑93 (MQ=255)
                                   gTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATc                                                      >  2:1752998/1‑93 (MQ=255)
                                     gCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGt                                                    <  1:8642040/93‑1 (MQ=255)
                                       aGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGa                                                  >  1:989162/1‑93 (MQ=255)
                                       aGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGa                                                  <  1:2025316/93‑1 (MQ=255)
                                          gcATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTa                                               >  2:9520057/1‑93 (MQ=255)
                                           cATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGCCAAGATAAATAAAGACGACATCGTGATTaa                                              >  1:5548680/1‑93 (MQ=255)
                                           cATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTaa                                              >  1:3201756/1‑93 (MQ=255)
                                           cATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTaa                                              >  1:1194487/1‑93 (MQ=255)
                                           cATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTaa                                              >  1:7131282/1‑93 (MQ=255)
                                            atatACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAAc                                             >  1:2543653/1‑93 (MQ=255)
                                             tataCGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACg                                            >  1:4250259/1‑93 (MQ=255)
                                             tataCGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACg                                            >  1:6013271/1‑93 (MQ=255)
                                             tataCGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACg                                            >  2:4900047/1‑93 (MQ=255)
                                                aCGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGata                                         <  1:8553537/93‑1 (MQ=255)
                                                aCGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGata                                         <  1:3464099/93‑1 (MQ=255)
                                                  gTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATc                                       <  2:6528148/93‑1 (MQ=255)
                                                  gTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATc                                       >  2:5178580/1‑93 (MQ=255)
                                                  gTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATc                                       <  2:8721198/93‑1 (MQ=255)
                                                  gTGGAGAACAAAATGTCATGTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATc                                       <  2:5295565/93‑1 (MQ=255)
                                                   tGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATcg                                      <  2:9703142/93‑1 (MQ=255)
                                                      agaACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCgg                                   >  2:8828057/1‑93 (MQ=255)
                                                      agaACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCgg                                   >  2:103709/1‑93 (MQ=255)
                                                      agaACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCgg                                   >  1:4095365/1‑93 (MQ=255)
                                                       gaACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGCGATTAACGATATCGCGGt                                  >  1:951146/1‑93 (MQ=255)
                                                        aaCAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGtt                                 <  2:8062816/93‑1 (MQ=255)
                                                            aaaTGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAACGACGACATCGTGATTACCGATATCGCGGTTTccc                             >  2:1076661/1‑93 (MQ=255)
                                                              aTGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCtt                           <  2:7849302/93‑1 (MQ=255)
                                                                  cATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCaa                       <  1:2601250/93‑1 (MQ=255)
                                                                   atttatttAAACTTTTTCCAGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATAGCGCGGTTTCCCTTTCaaa                      >  1:6432877/1‑93 (MQ=255)
                                                                   aTTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCaaa                      <  1:2400018/93‑1 (MQ=255)
                                                                     ttattaAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAata                    <  2:1365928/93‑1 (MQ=255)
                                                                       attaAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATc                  <  2:161928/93‑1 (MQ=255)
                                                                         taAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTg                >  2:5752881/1‑93 (MQ=255)
                                                                         taAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTg                <  2:9415868/93‑1 (MQ=255)
                                                                          aaaaCTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGc               >  2:9089346/1‑93 (MQ=255)
                                                                           aaaCTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGcc              >  1:4706679/1‑93 (MQ=255)
                                                                           aaaCTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGcc              <  1:9169571/93‑1 (MQ=255)
                                                                            aaCTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCg             >  2:3727029/1‑93 (MQ=255)
                                                                               tttttCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCtt          >  1:8864361/1‑93 (MQ=255)
                                                                               tttttCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGGGATTAAGGATATCGCGGTTTCCCTTTCAAATACCTGCCGCtt          >  1:1085699/1‑93 (MQ=255)
                                                                                  ttCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGc       <  2:5900278/93‑1 (MQ=255)
                                                                                      gggAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGt   >  2:6073409/1‑93 (MQ=255)
                                                                                       ggAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCCAATATCTGCCGCTTTGCCGGTc  <  1:1825071/93‑1 (MQ=255)
                                                                                       ggAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTc  <  2:8902955/93‑1 (MQ=255)
                                                                                       ggAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTc  <  1:575495/93‑1 (MQ=255)
                                                                                          |                                                                                         
ATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTC  >  NC_000913/2472942‑2473121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 206 total aligned reads displayed.
CGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCT  >  NC_000913/2472939‑2473125
                                                                                             |                                                                                             
CGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGCACAAAATGTCATTTATTAACACTTTTTCCGGGAG                                                                                               >  SRR4302540.6487853/1‑94 (MQ=60)
CGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAG                                                                                               <  SRR4302540.4293344/94‑1 (MQ=60)
CGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAG                                                                                               <  SRR4302540.664185/94‑1 (MQ=60)
CGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAG                                                                                               >  SRR4302540.4658832/1‑94 (MQ=60)
 GGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGG                                                                                              <  SRR4302540.4280102/94‑1 (MQ=60)
 GGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGG                                                                                              <  SRR4302540.3358202/94‑1 (MQ=60)
  GATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAACCTTTTTCCGGGAGGC                                                                                             <  SRR4302540.7469850/94‑1 (MQ=60)
   ATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCA                                                                                            >  SRR4302540.8254925/1‑94 (MQ=60)
   ATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCA                                                                                            >  SRR4302540.6318990/1‑94 (MQ=60)
   ATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCA                                                                                            <  SRR4302540.2535047/94‑1 (MQ=60)
    TGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCAT                                                                                           <  SRR4302540.8638107/94‑1 (MQ=60)
    TGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGAGAGGCAT                                                                                           >  SRR4302540.9428544/1‑94 (MQ=60)
     TGTGAGGGCTTACTTTTACCCAAACTCAGCGCGGTGCAGATCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATT                                                                                          <  SRR4302540.1572186/94‑1 (MQ=60)
     GGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATT                                                                                          >  SRR4302540.7214881/1‑94 (MQ=60)
      GTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTT                                                                                         <  SRR4302540.5090528/94‑1 (MQ=60)
      GTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTT                                                                                         >  SRR4302540.3097738/1‑94 (MQ=60)
       TGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTT                                                                                        >  SRR4302540.6278247/1‑94 (MQ=60)
       TGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTT                                                                                        <  SRR4302540.1348570/94‑1 (MQ=60)
       TGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTT                                                                                        >  SRR4302540.7866852/1‑94 (MQ=60)
        GAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTT                                                                                       <  SRR4302540.5289422/94‑1 (MQ=60)
          GGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTAT                                                                                     >  SRR4302540.1535373/1‑94 (MQ=60)
          GGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTAT                                                                                     <  SRR4302540.2577458/94‑1 (MQ=60)
          GGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTAT                                                                                     <  SRR4302540.2577457/94‑1 (MQ=60)
          GGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTAT                                                                                     <  SRR4302540.3342971/94‑1 (MQ=60)
           GGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATT                                                                                    >  SRR4302540.3024128/1‑94 (MQ=60)
            GCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTA                                                                                   <  SRR4302540.8446336/94‑1 (MQ=60)
            GCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTA                                                                                   >  SRR4302540.7623303/1‑94 (MQ=60)
            GCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTA                                                                                   >  SRR4302540.8697392/1‑94 (MQ=60)
             CTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTAT                                                                                  <  SRR4302540.2067703/94‑1 (MQ=60)
             CTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTAT                                                                                  <  SRR4302540.4113907/94‑1 (MQ=60)
             CTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTAT                                                                                  >  SRR4302540.4226943/1‑94 (MQ=60)
              TTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATG                                                                                 <  SRR4302540.3603336/94‑1 (MQ=60)
              TTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATG                                                                                 >  SRR4302540.4177758/1‑94 (MQ=60)
              TTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATG                                                                                 <  SRR4302540.1499646/94‑1 (MQ=60)
               TCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGA                                                                                >  SRR4302540.988919/1‑94 (MQ=60)
               TCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGA                                                                                <  SRR4302540.2312040/94‑1 (MQ=60)
               TCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGA                                                                                <  SRR4302540.9886123/94‑1 (MQ=60)
               TCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGA                                                                                <  SRR4302540.1270752/94‑1 (MQ=60)
               TCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGA                                                                                >  SRR4302540.3764676/1‑94 (MQ=60)
               TCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGA                                                                                <  SRR4302540.322874/94‑1 (MQ=60)
               TCCTGTTACACACACTCAGCGCGCTGCCGCGCATATACGTGGAGAACAAAATGCCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGA                                                                                <  SRR4302540.322613/94‑1 (MQ=60)
                CCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGAC                                                                               <  SRR4302540.6725175/94‑1 (MQ=60)
                 CTTTTACCTAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACA                                                                              >  SRR4302540.2719801/1‑94 (MQ=60)
                 CTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACA                                                                              <  SRR4302540.1468761/94‑1 (MQ=60)
                 CTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACA                                                                              >  SRR4302540.10050886/1‑94 (MQ=60)
                 CTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACA                                                                              >  SRR4302540.9706963/1‑94 (MQ=60)
                 CTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACA                                                                              >  SRR4302540.3589450/1‑94 (MQ=60)
                 CTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACA                                                                              >  SRR4302540.3192649/1‑94 (MQ=60)
                 CTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACA                                                                              >  SRR4302540.8425450/1‑94 (MQ=60)
                   TTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAG                                                                            <  SRR4302540.4110526/94‑1 (MQ=60)
                   TTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAG                                                                            <  SRR4302540.800997/94‑1 (MQ=60)
                    TTACGCAAACACAGCGCGGTGCAGCGCATATACGTGGAGAACATAATGTCATTTATTAAACCTTTTTCCGGGAGGCATTTTTATTATGACAAGA                                                                           <  SRR4302540.9160199/94‑1 (MQ=60)
                    TTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGA                                                                           <  SRR4302540.5536423/94‑1 (MQ=60)
                    TTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGA                                                                           <  SRR4302540.9159946/94‑1 (MQ=60)
                     TACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGAT                                                                          >  SRR4302540.6386676/1‑94 (MQ=60)
                      ACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATA                                                                         <  SRR4302540.3558377/94‑1 (MQ=60)
                       CCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAA                                                                        >  SRR4302540.8941689/1‑94 (MQ=60)
                       CCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAA                                                                        >  SRR4302540.7663101/1‑94 (MQ=60)
                       CCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACCTTTTCCGGGAGGCATTTTTATTATGACAAGATAA                                                                        >  SRR4302540.8941401/1‑94 (MQ=60)
                        CCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAA                                                                       >  SRR4302540.1512650/1‑94 (MQ=60)
                        CCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAA                                                                       <  SRR4302540.5372637/94‑1 (MQ=60)
                         CAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAAT                                                                      <  SRR4302540.3226064/94‑1 (MQ=60)
                         CAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAAT                                                                      >  SRR4302540.2023991/1‑94 (MQ=60)
                         CAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAAT                                                                      <  SRR4302540.4476005/94‑1 (MQ=60)
                         CAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAAT                                                                      <  SRR4302540.4476006/94‑1 (MQ=60)
                          AAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATA                                                                     <  SRR4302540.5643877/94‑1 (MQ=60)
                          AAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATAACAAGATAAATA                                                                     <  SRR4302540.7476244/94‑1 (MQ=60)
                          AAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATA                                                                     >  SRR4302540.6625672/1‑94 (MQ=60)
                           AACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAA                                                                    <  SRR4302540.441702/94‑1 (MQ=60)
                           AACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATATATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAA                                                                    <  SRR4302540.8696545/94‑1 (MQ=60)
                            ACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAA                                                                   >  SRR4302540.3757447/1‑94 (MQ=60)
                            ACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAA                                                                   <  SRR4302540.3568132/94‑1 (MQ=60)
                               CAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGAC                                                                <  SRR4302540.4545630/94‑1 (MQ=60)
                               CAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGAC                                                                >  SRR4302540.10008231/1‑94 (MQ=60)
                                  CGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGAC                                                             <  SRR4302540.9751660/94‑1 (MQ=60)
                                  CGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGAC                                                             <  SRR4302540.7629200/94‑1 (MQ=60)
                                  CGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGAC                                                             <  SRR4302540.9751659/94‑1 (MQ=60)
                                  CGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGAC                                                             <  SRR4302540.7629201/94‑1 (MQ=60)
                                  CGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGAC                                                             >  SRR4302540.7909242/1‑94 (MQ=60)
                                  CGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGAC                                                             <  SRR4302540.7664199/94‑1 (MQ=60)
                                  CGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGAC                                                             <  SRR4302540.7664200/94‑1 (MQ=60)
                                  CGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGAA                                                             >  SRR4302540.5124677/1‑94 (MQ=60)
                                   GCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACA                                                            <  SRR4302540.2834565/94‑1 (MQ=60)
                                   GCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACA                                                            >  SRR4302540.5962072/1‑94 (MQ=60)
                                   GCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACA                                                            <  SRR4302540.4692811/94‑1 (MQ=60)
                                     GGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATC                                                          <  SRR4302540.3036733/94‑1 (MQ=60)
                                     GGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATC                                                          <  SRR4302540.2809786/94‑1 (MQ=60)
                                     GGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATC                                                          >  SRR4302540.1380377/1‑94 (MQ=60)
                                     GGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATC                                                          >  SRR4302540.9384838/1‑94 (MQ=60)
                                       TGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGT                                                        <  SRR4302540.10026887/94‑1 (MQ=60)
                                        GCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTG                                                       <  SRR4302540.9537589/94‑1 (MQ=60)
                                        GCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTG                                                       <  SRR4302540.8742426/94‑1 (MQ=60)
                                        GCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTG                                                       >  SRR4302540.841117/1‑94 (MQ=60)
                                          AGCGCATATACGTGGAGAACAAAATGTCATTTATTAAACCTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGAT                                                     <  SRR4302540.9257157/94‑1 (MQ=60)
                                          AGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGAT                                                     >  SRR4302540.6042022/1‑94 (MQ=60)
                                          AGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGAT                                                     <  SRR4302540.2047196/94‑1 (MQ=60)
                                          AGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGAT                                                     >  SRR4302540.445584/1‑94 (MQ=60)
                                            CGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTA                                                   >  SRR4302540.6064759/1‑94 (MQ=60)
                                            CGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTA                                                   <  SRR4302540.7011349/94‑1 (MQ=60)
                                            CGCATATACGTGGAGAACAAAATGTCATTGATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTA                                                   <  SRR4302540.7011348/94‑1 (MQ=60)
                                             GCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGCCAAGATAAATAAAGACGACATCGTGATTAA                                                  >  SRR4302540.5609805/1‑94 (MQ=60)
                                             GCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAA                                                  <  SRR4302540.3988776/94‑1 (MQ=60)
                                             GCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAA                                                  >  SRR4302540.3236525/1‑94 (MQ=60)
                                             GCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAA                                                  >  SRR4302540.6714797/1‑94 (MQ=60)
                                             GCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAA                                                  >  SRR4302540.1206791/1‑94 (MQ=60)
                                             GCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAA                                                  >  SRR4302540.7210722/1‑94 (MQ=60)
                                              CATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAAC                                                 <  SRR4302540.5887084/94‑1 (MQ=60)
                                              CATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAAC                                                 <  SRR4302540.4044583/94‑1 (MQ=60)
                                              CATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAAC                                                 >  SRR4302540.2571075/1‑94 (MQ=60)
                                              CATATACGTGGAGAACAAAATGTCAGTTATTAAAACTTTTTCCGGAAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAAC                                                 >  SRR4302540.4835778/1‑94 (MQ=60)
                                               ATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACG                                                <  SRR4302540.1211018/94‑1 (MQ=60)
                                               ATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACG                                                >  SRR4302540.4296498/1‑94 (MQ=60)
                                               ATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACG                                                >  SRR4302540.451987/1‑94 (MQ=60)
                                               ATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACG                                                >  SRR4302540.6079955/1‑94 (MQ=60)
                                               ATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACG                                                >  SRR4302540.6079956/1‑94 (MQ=60)
                                                 ATACGTGGAGAACAAAATGTCATTTATTAAATCTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGAT                                              <  SRR4302540.9366622/94‑1 (MQ=60)
                                                 ATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGAT                                              <  SRR4302540.293234/94‑1 (MQ=60)
                                                 ATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGAT                                              <  SRR4302540.2866585/94‑1 (MQ=60)
                                                 ATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGAT                                              <  SRR4302540.8801065/94‑1 (MQ=60)
                                                  TACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATA                                             <  SRR4302540.734655/94‑1 (MQ=60)
                                                  TACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATA                                             <  SRR4302540.4309166/94‑1 (MQ=60)
                                                  TACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGAAGACATCGTGATTAACGATA                                             >  SRR4302540.4941525/1‑94 (MQ=60)
                                                   ACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATAT                                            <  SRR4302540.5344167/94‑1 (MQ=60)
                                                   ACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATAT                                            <  SRR4302540.3688672/94‑1 (MQ=60)
                                                   ACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATAT                                            <  SRR4302540.3501806/94‑1 (MQ=60)
                                                    CGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATC                                           >  SRR4302540.9245455/1‑94 (MQ=60)
                                                    CGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATC                                           <  SRR4302540.1950197/94‑1 (MQ=60)
                                                    CGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATC                                           <  SRR4302540.871924/94‑1 (MQ=60)
                                                    CGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATC                                           <  SRR4302540.4122419/94‑1 (MQ=60)
                                                     GTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCG                                          >  SRR4302540.1153530/1‑94 (MQ=60)
                                                     GTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCG                                          <  SRR4302540.8231925/94‑1 (MQ=60)
                                                     GTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCG                                          >  SRR4302540.4197618/1‑94 (MQ=60)
                                                        GAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGG                                       >  SRR4302540.4139549/1‑94 (MQ=60)
                                                        GAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGG                                       >  SRR4302540.5654992/1‑94 (MQ=60)
                                                         AGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGT                                      >  SRR4302540.6404888/1‑94 (MQ=60)
                                                         AGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGT                                      >  SRR4302540.9078500/1‑94 (MQ=60)
                                                         AGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGCGATTAACGATATCGCGGT                                      >  SRR4302540.961029/1‑94 (MQ=60)
                                                           AACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTT                                    <  SRR4302540.6980785/94‑1 (MQ=60)
                                                           AACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTT                                    <  SRR4302540.2909531/94‑1 (MQ=60)
                                                            ACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTC                                   >  SRR4302540.5978680/1‑94 (MQ=60)
                                                            ACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTC                                   <  SRR4302540.8086515/94‑1 (MQ=60)
                                                             CAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCC                                  <  SRR4302540.4467192/94‑1 (MQ=60)
                                                             CAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCC                                  >  SRR4302540.5169061/1‑94 (MQ=60)
                                                               AAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCT                                >  SRR4302540.3192014/1‑94 (MQ=60)
                                                               AAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCT                                <  SRR4302540.4122715/94‑1 (MQ=60)
                                                               AAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCT                                >  SRR4302540.9876338/1‑94 (MQ=60)
                                                               AAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCT                                >  SRR4302540.5225303/1‑94 (MQ=60)
                                                                 ATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTT                              >  SRR4302540.1495425/1‑94 (MQ=60)
                                                                 ATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTT                              <  SRR4302540.9954549/94‑1 (MQ=60)
                                                                 ATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTT                              <  SRR4302540.9954550/94‑1 (MQ=60)
                                                                 ATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTT                              >  SRR4302540.6895915/1‑94 (MQ=60)
                                                                 ATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTT                              <  SRR4302540.2156429/94‑1 (MQ=60)
                                                                   TTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCA                            <  SRR4302540.8242866/94‑1 (MQ=60)
                                                                   GTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCA                            <  SRR4302540.3654296/94‑1 (MQ=60)
                                                                   GTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCA                            <  SRR4302540.4353145/94‑1 (MQ=60)
                                                                     CATTTATTTAAACTTTTTCCAGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATAGCGCGGTTTCCCTTTCAAA                          >  SRR4302540.6504477/1‑94 (MQ=60)
                                                                     CATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAA                          >  SRR4302540.8471763/1‑94 (MQ=60)
                                                                     CATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAA                          <  SRR4302540.2629305/94‑1 (MQ=60)
                                                                     CATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAA                          >  SRR4302540.9282761/1‑94 (MQ=60)
                                                                      ATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAAT                         <  SRR4302540.2425895/94‑1 (MQ=60)
                                                                      ATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAAT                         >  SRR4302540.2755158/1‑94 (MQ=60)
                                                                      ATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAAT                         <  SRR4302540.8297480/94‑1 (MQ=60)
                                                                      ATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAAT                         <  SRR4302540.6764862/94‑1 (MQ=60)
                                                                      ATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAAT                         >  SRR4302540.4416076/1‑94 (MQ=60)
                                                                      ATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAAT                         <  SRR4302540.5885118/94‑1 (MQ=60)
                                                                       TTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATA                        >  SRR4302540.8327974/1‑94 (MQ=60)
                                                                       TTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATA                        >  SRR4302540.9560577/1‑94 (MQ=60)
                                                                       TTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATA                        >  SRR4302540.9560578/1‑94 (MQ=60)
                                                                        TTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATAT                       <  SRR4302540.5947873/94‑1 (MQ=60)
                                                                         TATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATC                      >  SRR4302540.6786261/1‑94 (MQ=60)
                                                                         TATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATC                      >  SRR4302540.7421952/1‑94 (MQ=60)
                                                                         TATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATC                      <  SRR4302540.197443/94‑1 (MQ=60)
                                                                          ATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCT                     <  SRR4302540.9782456/94‑1 (MQ=60)
                                                                           TTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTG                    <  SRR4302540.485472/94‑1 (MQ=60)
                                                                            TAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGC                   >  SRR4302540.5723481/1‑94 (MQ=60)
                                                                            TAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGC                   >  SRR4302540.6246879/1‑94 (MQ=60)
                                                                             AAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCC                  >  SRR4302540.4756974/1‑94 (MQ=60)
                                                                             AAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCC                  >  SRR4302540.3401236/1‑94 (MQ=60)
                                                                              AAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCG                 <  SRR4302540.9279572/94‑1 (MQ=60)
                                                                                 CTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTT              >  SRR4302540.8966935/1‑94 (MQ=60)
                                                                                 CTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTT              >  SRR4302540.1096829/1‑94 (MQ=60)
                                                                                 CTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGGGATTAAGGATATCGCGGTTTCCCTTTCAAATACCTGCCGCTT              >  SRR4302540.1096827/1‑94 (MQ=60)
                                                                                  TTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTT             <  SRR4302540.6433501/94‑1 (MQ=60)
                                                                                  TTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTT             <  SRR4302540.10005417/94‑1 (MQ=60)
                                                                                   TTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTG            <  SRR4302540.8008645/94‑1 (MQ=60)
                                                                                      TCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCG         <  SRR4302540.1213368/94‑1 (MQ=60)
                                                                                       CCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGG        <  SRR4302540.9738071/94‑1 (MQ=60)
                                                                                        CGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGT       >  SRR4302540.9112565/1‑94 (MQ=60)
                                                                                        CGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGT       <  SRR4302540.3470508/94‑1 (MQ=60)
                                                                                        CGGGAAGCATTTTTATTATGACAAGATAAATACAGACGACATCGTGATTAACGATATCGCGGTTTACCTTTCAAATATCTGCCACTTTGCCGtc       >  SRR4302540.7930431/1‑92 (MQ=60)
                                                                                         GGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTC      >  SRR4302540.2536265/1‑94 (MQ=60)
                                                                                         GGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTC      >  SRR4302540.2536264/1‑94 (MQ=60)
                                                                                          GGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCCAATATCTGCCGCTTTGCCGGTCA     <  SRR4302540.1845028/94‑1 (MQ=60)
                                                                                          GGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCA     >  SRR4302540.7464864/1‑94 (MQ=60)
                                                                                          GGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCA     <  SRR4302540.6547139/94‑1 (MQ=60)
                                                                                          GGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCA     <  SRR4302540.581735/94‑1 (MQ=60)
                                                                                           GAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCAT    >  SRR4302540.8294533/1‑94 (MQ=60)
                                                                                           GAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCAT    >  SRR4302540.1999575/1‑94 (MQ=60)
                                                                                            AGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATC   <  SRR4302540.1942577/94‑1 (MQ=60)
                                                                                             GGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCT  <  SRR4302540.3352261/94‑1 (MQ=60)
                                                                                             |                                                                                             
CGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCATCT  >  NC_000913/2472939‑2473125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑