Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,473,032 A→G K19R (AAG→AGG)  b2361 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,473,0320AG100.0% 696.3 / NA 213K19R (AAG→AGG) b2361hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (109/104);  total (109/104)

Only 100 of 215 total aligned reads displayed.

ATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTC  >  NC_000913/2472942‑2473121
                                                                                          |                                                                                         
aTGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGc                                                                                         >  1:4344486/1‑93 (MQ=255)
   gTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCAtt                                                                                      >  1:3025104/1‑93 (MQ=255)
       gggCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTtta                                                                                  >  2:1711803/1‑93 (MQ=255)
        ggCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTttat                                                                                 <  1:3725530/93‑1 (MQ=255)
         gCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTttatt                                                                                <  1:4041713/93‑1 (MQ=255)
         gCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTttatt                                                                                >  1:5297030/1‑93 (MQ=255)
          cTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTttatta                                                                               >  2:5579705/1‑93 (MQ=255)
          cTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTttatta                                                                               >  1:5933210/1‑93 (MQ=255)
           ttCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATCTACGGGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTtattat                                                                              >  2:1826971/1‑93 (MQ=255)
           ttCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTtattat                                                                              <  2:2834791/93‑1 (MQ=255)
           ttCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTtattat                                                                              >  1:3150373/1‑93 (MQ=255)
           ttCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTtattat                                                                              <  2:2271198/93‑1 (MQ=255)
            tCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATg                                                                             >  1:868639/1‑93 (MQ=255)
            tCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATg                                                                             <  2:3066657/93‑1 (MQ=255)
            tCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATg                                                                             <  2:2582764/93‑1 (MQ=255)
                tttACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACaa                                                                         <  1:816330/93‑1 (MQ=255)
                tttACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACaa                                                                         >  2:2143557/1‑93 (MQ=255)
                tttACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGACATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACaa                                                                         <  1:4352596/93‑1 (MQ=255)
                    cccAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGata                                                                     <  2:3567423/93‑1 (MQ=255)
                    cccAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGata                                                                     >  1:4891844/1‑93 (MQ=255)
                     ccAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGataa                                                                    <  2:3222521/93‑1 (MQ=255)
                     ccAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGataa                                                                    <  2:4850944/93‑1 (MQ=255)
                        aaCTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGataaata                                                                 <  1:3901386/93‑1 (MQ=255)
                         aCTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGataaataa                                                                <  1:941576/93‑1 (MQ=255)
                          cTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGAtaaataaa                                                               <  1:2979430/93‑1 (MQ=255)
                          cTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGAtaaataaa                                                               <  2:1529345/93‑1 (MQ=255)
                          cTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGAtaaataaa                                                               <  1:1754113/93‑1 (MQ=255)
                           tCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAg                                                              >  2:3979705/1‑93 (MQ=255)
                            cAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAga                                                             <  1:2895805/93‑1 (MQ=255)
                            cAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAga                                                             >  2:23334/1‑93 (MQ=255)
                            cAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAga                                                             >  1:1452139/1‑93 (MQ=255)
                            cAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAga                                                             <  2:3380013/93‑1 (MQ=255)
                             aGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAgac                                                            >  1:2429921/1‑93 (MQ=255)
                             aGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAgac                                                            <  2:255023/93‑1 (MQ=255)
                             aGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAgac                                                            <  1:5793654/93‑1 (MQ=255)
                              gcgcGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAgacg                                                           >  1:1110214/1‑93 (MQ=255)
                                 cgGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAGAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACa                                                        <  2:5962852/93‑1 (MQ=255)
                                 cgGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACa                                                        >  2:2543931/1‑93 (MQ=255)
                                 cgGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACa                                                        <  1:2377866/93‑1 (MQ=255)
                                 cgGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACa                                                        <  1:4992098/93‑1 (MQ=255)
                                  ggTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACAt                                                       >  1:2077153/1‑93 (MQ=255)
                                  ggTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACAt                                                       <  2:870983/93‑1 (MQ=255)
                                  ggTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACAt                                                       <  1:5521271/93‑1 (MQ=255)
                                  ggTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACAt                                                       <  1:5117528/93‑1 (MQ=255)
                                    tGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCg                                                     <  2:4783585/93‑1 (MQ=255)
                                        gcgcATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGAt                                                 <  2:4631341/93‑1 (MQ=255)
                                        gcgcATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGAt                                                 >  2:5677833/1‑93 (MQ=255)
                                         cgcATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGAtt                                                <  2:6052336/93‑1 (MQ=255)
                                          gcATATACGTGGAGAACAAAATGTCATTTATTAAACCTTTTTCCGGGAGGCATTTTTA‑TATGACAAGATAAATAAAGACGACATCGTGATTaa                                              <  1:380659/93‑1 (MQ=255)
                                            atatACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAAc                                             >  1:5224313/1‑93 (MQ=255)
                                             tataCGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACg                                            >  1:3522836/1‑93 (MQ=255)
                                             tataCGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACg                                            >  1:3479123/1‑93 (MQ=255)
                                             tataCGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACg                                            >  1:2163026/1‑93 (MQ=255)
                                              ataCGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGa                                           >  1:1559895/1‑93 (MQ=255)
                                                aCGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGata                                         <  2:3932989/93‑1 (MQ=255)
                                                 cGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGatat                                        <  1:612260/93‑1 (MQ=255)
                                                 cGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGatat                                        <  2:5739003/93‑1 (MQ=255)
                                                  gTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATc                                       <  2:6235823/93‑1 (MQ=255)
                                                   tGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATcg                                      >  1:4468348/1‑93 (MQ=255)
                                                    ggAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATcgc                                     >  1:101222/1‑93 (MQ=255)
                                                    ggAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATcgc                                     >  2:2012602/1‑93 (MQ=255)
                                                    ggAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATcgc                                     <  2:5482827/93‑1 (MQ=255)
                                                    ggAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATcgc                                     >  1:3992128/1‑93 (MQ=255)
                                                      agaACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCgg                                   <  1:1002996/93‑1 (MQ=255)
                                                      agaACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCgg                                   >  2:6248835/1‑93 (MQ=255)
                                                      agaACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCgg                                   <  2:3354893/93‑1 (MQ=255)
                                                      agaACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCgg                                   <  2:871191/93‑1 (MQ=255)
                                                      agaACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCgg                                   >  2:1900119/1‑93 (MQ=255)
                                                       gaACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGt                                  <  2:520054/93‑1 (MQ=255)
                                                         aCAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGttt                                >  1:763954/1‑93 (MQ=255)
                                                          cAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTc                               >  1:1831590/1‑93 (MQ=255)
                                                          cAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTc                               >  2:4290135/1‑93 (MQ=255)
                                                           aaaaTGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTcc                              >  2:3284242/1‑93 (MQ=255)
                                                            aaaTGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTccc                             <  1:1643478/93‑1 (MQ=255)
                                                             aaTGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCt                            >  1:1958881/1‑93 (MQ=255)
                                                             aaTGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCt                            <  2:5365427/93‑1 (MQ=255)
                                                               tGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCttt                          >  1:837623/1‑93 (MQ=255)
                                                               tGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCttt                          >  2:1003630/1‑93 (MQ=255)
                                                               tGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCttt                          >  2:714329/1‑93 (MQ=255)
                                                                 tCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCa                        >  2:6267327/1‑93 (MQ=255)
                                                                 tCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCa                        >  1:2330395/1‑93 (MQ=255)
                                                                 tCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCa                        <  1:4778417/93‑1 (MQ=255)
                                                                 tCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCa                        >  1:465355/1‑93 (MQ=255)
                                                                  cATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCaa                       >  1:121625/1‑93 (MQ=255)
                                                                     ttattaAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAata                    <  2:3824661/93‑1 (MQ=255)
                                                                      tattaAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAatat                   >  2:302374/1‑93 (MQ=255)
                                                                       attaAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATc                  <  1:1188574/93‑1 (MQ=255)
                                                                          aaaaCTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGc               <  1:533018/93‑1 (MQ=255)
                                                                            aaCTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCg             <  2:3517516/93‑1 (MQ=255)
                                                                               tttttCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGCCATCGTAATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCtt          <  2:5889205/93‑1 (MQ=255)
                                                                               tttttCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCtt          <  1:2997700/93‑1 (MQ=255)
                                                                               tttttCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCtt          <  1:2541849/93‑1 (MQ=255)
                                                                               tttttCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCtt          >  2:5014952/1‑93 (MQ=255)
                                                                               tttttCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCtt          <  1:1211618/93‑1 (MQ=255)
                                                                                ttttCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCttt         <  2:2651829/93‑1 (MQ=255)
                                                                                   tCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGcc      <  1:3651601/93‑1 (MQ=255)
                                                                                       ggAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTc  <  1:2809984/93‑1 (MQ=255)
                                                                                       ggAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTc  <  2:5244954/93‑1 (MQ=255)
                                                                                       ggAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTc  >  1:5632650/1‑93 (MQ=255)
                                                                                       ggAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTc  >  2:1603572/1‑93 (MQ=255)
                                                                                          |                                                                                         
ATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTC  >  NC_000913/2472942‑2473121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCAT  >  NC_000913/2472939‑2473123
                                                                                             |                                                                                           
CGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAG                                                                                             >  SRR4302533.5959318/1‑94 (MQ=60)
CGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAG                                                                                             <  SRR4302533.3836722/94‑1 (MQ=60)
CGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAG                                                                                             <  SRR4302533.2609567/94‑1 (MQ=60)
CGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAG                                                                                             >  SRR4302533.5266560/1‑94 (MQ=60)
  GATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGC                                                                                           >  SRR4302533.4370668/1‑94 (MQ=60)
   ATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCA                                                                                          <  SRR4302533.2406427/94‑1 (MQ=60)
    TGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCAT                                                                                         >  SRR4302533.5121756/1‑94 (MQ=60)
     GGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATT                                                                                        >  SRR4302533.3043610/1‑94 (MQ=60)
     GGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATT                                                                                        <  SRR4302533.1209485/94‑1 (MQ=60)
     GGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATT                                                                                        <  SRR4302533.1594463/94‑1 (MQ=60)
      GTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTT                                                                                       <  SRR4302533.1055956/94‑1 (MQ=60)
         AGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTA                                                                                    >  SRR4302533.5868956/1‑94 (MQ=60)
          GGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTAT                                                                                   <  SRR4302533.4120475/94‑1 (MQ=60)
          GGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTAT                                                                                   <  SRR4302533.4440351/94‑1 (MQ=60)
           GGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATT                                                                                  <  SRR4302533.3748388/94‑1 (MQ=60)
           GGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATT                                                                                  >  SRR4302533.5328755/1‑94 (MQ=60)
            GCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTA                                                                                 >  SRR4302533.5969079/1‑94 (MQ=60)
            GCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTA                                                                                 <  SRR4302533.4066312/94‑1 (MQ=60)
             CTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTAT                                                                                <  SRR4302533.4941812/94‑1 (MQ=60)
             CTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTAT                                                                                >  SRR4302533.3169744/1‑94 (MQ=60)
              TTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATG                                                                               >  SRR4302533.873735/1‑94 (MQ=60)
                CCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGAC                                                                             >  SRR4302533.5173106/1‑94 (MQ=60)
                   TTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAG                                                                          <  SRR4302533.821114/94‑1 (MQ=60)
                   TTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGACATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAG                                                                          <  SRR4302533.4378862/94‑1 (MQ=60)
                   TTTACCCAAACTCAGCGCGGTGCAGCCCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAG                                                                          <  SRR4302533.838530/94‑1 (MQ=60)
                      ACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATA                                                                       >  SRR4302533.4921483/1‑94 (MQ=60)
                        CCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAA                                                                     >  SRR4302533.5359495/1‑94 (MQ=60)
                         CAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAAT                                                                    >  SRR4302533.2954804/1‑94 (MQ=60)
                         CAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAAT                                                                    >  SRR4302533.814313/1‑94 (MQ=60)
                           AACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAA                                                                  <  SRR4302533.3925206/94‑1 (MQ=60)
                            ACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAA                                                                 <  SRR4302533.947115/94‑1 (MQ=60)
                             CTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAG                                                                <  SRR4302533.1220222/94‑1 (MQ=60)
                             CTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAG                                                                >  SRR4302533.3135694/1‑94 (MQ=60)
                             CTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAG                                                                <  SRR4302533.1764797/94‑1 (MQ=60)
                             CTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAG                                                                <  SRR4302533.2997661/94‑1 (MQ=60)
                              TCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGA                                                               >  SRR4302533.1460901/1‑94 (MQ=60)
                               CAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGAC                                                              <  SRR4302533.4044189/94‑1 (MQ=60)
                               CAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGAC                                                              <  SRR4302533.2913613/94‑1 (MQ=60)
                               CAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGAC                                                              >  SRR4302533.2444941/1‑94 (MQ=60)
                                AGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACG                                                             <  SRR4302533.5828656/94‑1 (MQ=60)
                                AGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACG                                                             <  SRR4302533.1523614/94‑1 (MQ=60)
                                AGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACG                                                             >  SRR4302533.1116799/1‑94 (MQ=60)
                                  CGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGAC                                                           >  SRR4302533.1447107/1‑94 (MQ=60)
                                   GCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACA                                                          >  SRR4302533.6105163/1‑94 (MQ=60)
                                    CGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACAT                                                         >  SRR4302533.2089937/1‑94 (MQ=60)
                                    CGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACAT                                                         <  SRR4302533.235861/94‑1 (MQ=60)
                                    CGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACAT                                                         <  SRR4302533.5022363/94‑1 (MQ=60)
                                    CGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACAT                                                         <  SRR4302533.2392578/94‑1 (MQ=60)
                                    CGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACAT                                                         <  SRR4302533.3818726/94‑1 (MQ=60)
                                     GGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATC                                                        <  SRR4302533.5148452/94‑1 (MQ=60)
                                     GGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATC                                                        <  SRR4302533.5554605/94‑1 (MQ=60)
                                       TGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGT                                                      >  SRR4302533.1844659/1‑94 (MQ=60)
                                         CAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGA                                                    >  SRR4302533.3027661/1‑94 (MQ=60)
                                         CAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGA                                                    >  SRR4302533.6029263/1‑94 (MQ=60)
                                         CAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGA                                                    >  SRR4302533.5272095/1‑94 (MQ=60)
                                           GCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATT                                                  <  SRR4302533.2132094/94‑1 (MQ=60)
                                            CGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTA                                                 <  SRR4302533.928489/94‑1 (MQ=60)
                                             GCATATACGTGGAGAACAAAATGTCATTTATTAAACCTTTTTCCGGGAGGCATTTTTA‑TATGACAAGATAAATAAAGACGACATCGTGATTAAC                                               <  SRR4302533.382952/94‑1 (MQ=60)
                                              CATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAAC                                               >  SRR4302533.5255742/1‑94 (MQ=60)
                                               ATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACG                                              >  SRR4302533.3544551/1‑94 (MQ=60)
                                               ATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACG                                              >  SRR4302533.3500589/1‑94 (MQ=60)
                                               ATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACG                                              >  SRR4302533.2176337/1‑94 (MQ=60)
                                                TATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGA                                             >  SRR4302533.1569291/1‑94 (MQ=60)
                                                  TACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATA                                           >  SRR4302533.2312766/1‑94 (MQ=60)
                                                  TACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATA                                           <  SRR4302533.2547575/94‑1 (MQ=60)
                                                   ACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATAT                                          >  SRR4302533.4489462/1‑94 (MQ=60)
                                                   ACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATAT                                          <  SRR4302533.1009045/94‑1 (MQ=60)
                                                    CGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATC                                         <  SRR4302533.3598153/94‑1 (MQ=60)
                                                    CGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATC                                         >  SRR4302533.1787792/1‑94 (MQ=60)
                                                    CGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATC                                         <  SRR4302533.615860/94‑1 (MQ=60)
                                                     GTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCG                                        >  SRR4302533.4495373/1‑94 (MQ=60)
                                                      TGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGC                                       >  SRR4302533.4016438/1‑94 (MQ=60)
                                                      TGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGC                                       >  SRR4302533.101851/1‑94 (MQ=60)
                                                        GAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGG                                     >  SRR4302533.5794140/1‑94 (MQ=60)
                                                         AGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGT                                    >  SRR4302533.3993454/1‑94 (MQ=60)
                                                         AGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGT                                    <  SRR4302533.1008858/94‑1 (MQ=60)
                                                          GAACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTT                                   >  SRR4302533.3332718/1‑94 (MQ=60)
                                                           AACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTT                                  >  SRR4302533.768455/1‑94 (MQ=60)
                                                           AACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTT                                  <  SRR4302533.2881363/94‑1 (MQ=60)
                                                            ACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTC                                 >  SRR4302533.1842770/1‑94 (MQ=60)
                                                            ACAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTC                                 >  SRR4302533.1128584/1‑94 (MQ=60)
                                                             CAAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCC                                >  SRR4302533.3678207/1‑94 (MQ=60)
                                                              AAAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCC                               >  SRR4302533.3538430/1‑94 (MQ=60)
                                                               AAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCT                              <  SRR4302533.1653428/94‑1 (MQ=60)
                                                               AAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCT                              >  SRR4302533.1970838/1‑94 (MQ=60)
                                                               AAATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCT                              >  SRR4302533.1977693/1‑94 (MQ=60)
                                                                AATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTT                             >  SRR4302533.697271/1‑94 (MQ=60)
                                                                AATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTT                             <  SRR4302533.2915428/94‑1 (MQ=60)
                                                                 ATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTT                            <  SRR4302533.956856/94‑1 (MQ=60)
                                                                 ATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTT                            >  SRR4302533.842530/1‑94 (MQ=60)
                                                                 ATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTT                            >  SRR4302533.3859559/1‑94 (MQ=60)
                                                                 ATGTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTT                            <  SRR4302533.3014145/94‑1 (MQ=60)
                                                                   GTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCA                          >  SRR4302533.2344824/1‑94 (MQ=60)
                                                                   GTCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCA                          >  SRR4302533.468133/1‑94 (MQ=60)
                                                                    TCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAA                         >  SRR4302533.5490552/1‑94 (MQ=60)
                                                                    TCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAA                         >  SRR4302533.122392/1‑94 (MQ=60)
                                                                    TCATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAA                         <  SRR4302533.4807361/94‑1 (MQ=60)
                                                                     CATTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAA                        >  SRR4302533.5562122/1‑94 (MQ=60)
                                                                       TTTATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATA                      <  SRR4302533.2216976/94‑1 (MQ=60)
                                                                         TATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATC                    >  SRR4302533.4527945/1‑94 (MQ=60)
                                                                         TATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATC                    <  SRR4302533.1533686/94‑1 (MQ=60)
                                                                          ATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCT                   >  SRR4302533.1666363/1‑94 (MQ=60)
                                                                          ATTAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCT                   <  SRR4302533.1195709/94‑1 (MQ=60)
                                                                            TAAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGC                 >  SRR4302533.850073/1‑94 (MQ=60)
                                                                             AAAACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCC                <  SRR4302533.536187/94‑1 (MQ=60)
                                                                                ACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCT             >  SRR4302533.5202950/1‑94 (MQ=60)
                                                                                ACTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCT             >  SRR4302533.4728157/1‑94 (MQ=60)
                                                                                 CTTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTT            >  SRR4302533.4377030/1‑94 (MQ=60)
                                                                                  TTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTT           <  SRR4302533.3016029/94‑1 (MQ=60)
                                                                                  TTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTT           <  SRR4302533.2557496/94‑1 (MQ=60)
                                                                                  TTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTT           <  SRR4302533.1218908/94‑1 (MQ=60)
                                                                                  TTTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTT           >  SRR4302533.2623391/1‑94 (MQ=60)
                                                                                   TTTTCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTG          <  SRR4302533.3695193/94‑1 (MQ=60)
                                                                                      TCCGGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCG       <  SRR4302533.3674052/94‑1 (MQ=60)
                                                                                         GGGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTC    >  SRR4302533.5666711/1‑94 (MQ=60)
                                                                                         GGGAGGCATTTTTATTATGACAAGATAAATAAAGACCACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGTCGGTT    >  SRR4302533.4900308/1‑94 (MQ=60)
                                                                                          GGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCGAATATCTGCCGCTTTGCCGGTCA   >  SRR4302533.2554564/1‑94 (MQ=60)
                                                                                          GGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCA   <  SRR4302533.2827275/94‑1 (MQ=60)
                                                                                          GGAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCA   >  SRR4302533.5942724/1‑94 (MQ=60)
                                                                                           GAGGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCAT  >  SRR4302533.5250017/1‑94 (MQ=60)
                                                                                             |                                                                                           
CGGATGGTGAGGGCTTCCTTTTACCCAAACTCAGCGCGGTGCAGCGCATATACGTGGAGAACAAAATGTCATTTATTAAAACTTTTTCCGGGAAGCATTTTTATTATGACAAGATAAATAAAGACGACATCGTGATTAACGATATCGCGGTTTCCCTTTCAAATATCTGCCGCTTTGCCGGTCAT  >  NC_000913/2472939‑2473123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑