Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,488,381 A→G I530I (ATT→ATC)  b2373 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,488,3810AG99.7% 984.6 / NA 302I530I (ATT→ATC) b2373hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (102/199);  minor base C (0/1);  total (102/200)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 308 total aligned reads displayed.

TTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCT  >  NC_000913/2488291‑2488470
                                                                                          |                                                                                         
ttGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGCCCACATTAATAATGGTCGGTTTGCGCGACTGGAt                                                                                         <  2:3202564/93‑1 (MQ=255)
ttGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGAt                                                                                         <  2:7574523/93‑1 (MQ=255)
ttGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGAt                                                                                         <  2:8189540/93‑1 (MQ=255)
 tGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATa                                                                                        <  2:8425680/93‑1 (MQ=255)
  gTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATAc                                                                                       <  2:2722491/93‑1 (MQ=255)
   ttttGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATAcc                                                                                      >  1:5989650/1‑93 (MQ=255)
    tttGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCg                                                                                     <  1:4488697/93‑1 (MQ=255)
    tttGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCg                                                                                     <  1:3186887/93‑1 (MQ=255)
     ttGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCgg                                                                                    <  2:1098580/93‑1 (MQ=255)
      tGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCggg                                                                                   >  2:5458881/1‑92 (MQ=255)
       gggTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCggtg                                                                                  <  2:8660465/93‑1 (MQ=255)
          ttAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGtt                                                                               <  1:2628595/93‑1 (MQ=255)
           tAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTa                                                                              <  2:9171307/93‑1 (MQ=255)
            aaGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTaa                                                                             <  1:4712356/93‑1 (MQ=255)
               ttttGTAATATGCCCTCTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGc                                                                          <  2:3842100/93‑1 (MQ=255)
               tttGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGc                                                                          <  1:7591185/93‑1 (MQ=255)
                ttGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCa                                                                         >  1:6338883/1‑93 (MQ=255)
                ttGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCa                                                                         <  2:2029533/93‑1 (MQ=255)
                ttGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCa                                                                         <  1:6511325/93‑1 (MQ=255)
                 tGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCAt                                                                        >  1:68646/1‑93 (MQ=255)
                  ggTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATg                                                                       <  2:1247689/93‑1 (MQ=255)
                  ggTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATg                                                                       >  1:6412859/1‑93 (MQ=255)
                    tAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGAc                                                                     <  2:5207737/93‑1 (MQ=255)
                      atatGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGa                                                                   >  2:6212319/1‑93 (MQ=255)
                      atatGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGa                                                                   >  1:4218821/1‑93 (MQ=255)
                       tatGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGaa                                                                  <  2:6783419/93‑1 (MQ=255)
                       tatGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGaa                                                                  <  1:8827789/93‑1 (MQ=255)
                       tatGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGaa                                                                  <  2:1592563/93‑1 (MQ=255)
                       tatGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGaa                                                                  <  1:1726672/93‑1 (MQ=255)
                         tGGCAACTTGCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGt                                                                <  2:4282010/93‑1 (MQ=255)
                          ggCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGtt                                                               <  1:8478682/93‑1 (MQ=255)
                           gCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTc                                                              <  2:7003878/93‑1 (MQ=255)
                              ccTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATc                                                           <  2:1705617/92‑1 (MQ=255)
                              aCTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATc                                                           <  1:3993139/93‑1 (MQ=255)
                              aCTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATc                                                           <  2:7892790/93‑1 (MQ=255)
                              aCTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATc                                                           <  2:6423322/93‑1 (MQ=255)
                              aCTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATc                                                           <  2:4829564/93‑1 (MQ=255)
                              aCTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATc                                                           >  1:8168795/1‑93 (MQ=255)
                              aCTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGCTAAAGCATGACGAAGTTCATc                                                           >  2:8852992/1‑93 (MQ=255)
                               cTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCt                                                          <  2:8151626/93‑1 (MQ=255)
                               cTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCt                                                          <  2:7645323/93‑1 (MQ=255)
                                tttCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTg                                                         >  1:1304179/1‑93 (MQ=255)
                                tttCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTg                                                         <  1:6995979/93‑1 (MQ=255)
                                 ttCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGt                                                        >  1:863083/1‑93 (MQ=255)
                                 ttCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGt                                                        <  1:3570242/93‑1 (MQ=255)
                                  tCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTc                                                       <  1:8316787/93‑1 (MQ=255)
                                  tCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTc                                                       <  1:2470212/93‑1 (MQ=255)
                                   cAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGGGGTTAAAGCATGACGAAGTTCATCTGTCg                                                      >  1:4932018/1‑93 (MQ=255)
                                    aGTTCCGGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgt                                                     <  2:4176769/93‑1 (MQ=255)
                                     gTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgtg                                                    >  2:8446889/1‑93 (MQ=255)
                                       tCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgtggt                                                  <  1:5124567/93‑1 (MQ=255)
                                        ccTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgtggtg                                                 <  1:1890288/93‑1 (MQ=255)
                                          tgctgcAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGAc                                               >  1:128987/1‑93 (MQ=255)
                                            ctgcAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGt                                             >  2:3033004/1‑93 (MQ=255)
                                              gcAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTta                                           >  1:1060019/1‑93 (MQ=255)
                                              gcAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTta                                           <  1:9920844/93‑1 (MQ=255)
                                              gcAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTta                                           >  1:8746878/1‑93 (MQ=255)
                                                 gtgTCGATGACGCCATGAATAATGGTCGGTTTGCGCGACTGGATACCGGTGTTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAg                                        <  2:1452603/91‑1 (MQ=255)
                                                   gTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGcc                                      <  1:2981009/93‑1 (MQ=255)
                                                    tCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCa                                     <  2:724944/93‑1 (MQ=255)
                                                    tCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGAATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCa                                     <  1:5694103/93‑1 (MQ=255)
                                                     cGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCaa                                    <  2:2253036/93‑1 (MQ=255)
                                                      caTGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAAc                                   <  1:3264227/92‑1 (MQ=255)
                                                       aTGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACg                                  <  2:1725658/93‑1 (MQ=255)
                                                       aTGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACg                                  >  1:3139126/1‑93 (MQ=255)
                                                         gACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGcc                                >  1:4596649/1‑93 (MQ=255)
                                                         gACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGcc                                >  1:34494/1‑93 (MQ=255)
                                                          aCCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGTTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCa                               <  2:7401691/93‑1 (MQ=255)
                                                           ccACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCAc                              >  1:7072304/1‑93 (MQ=255)
                                                            cacaTTAATACTGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCccacgccacg                             >  2:5694700/1‑93 (MQ=255)
                                                             acaTTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGa                            >  2:4461168/1‑93 (MQ=255)
                                                               aTTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGaaa                          <  1:934318/93‑1 (MQ=255)
                                                                  aataatGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGc                       <  2:6903480/93‑1 (MQ=255)
                                                                   ataatGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCa                      <  1:6726456/93‑1 (MQ=255)
                                                                   ataatGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCa                      <  1:7986898/93‑1 (MQ=255)
                                                                   ataatGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCa                      <  2:7141605/93‑1 (MQ=255)
                                                                   ataatGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCa                      >  1:3401699/1‑93 (MQ=255)
                                                                   ataatGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCa                      <  2:7528654/93‑1 (MQ=255)
                                                                    taatGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCac                     >  1:2131345/1‑92 (MQ=255)
                                                                    taatGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCAt                     >  1:9542582/1‑93 (MQ=255)
                                                                       tGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCa                  <  2:7481758/93‑1 (MQ=255)
                                                                       tGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCa                  <  2:9734694/93‑1 (MQ=255)
                                                                          tCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTa               >  1:7181828/1‑93 (MQ=255)
                                                                           cGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTaa              <  1:2261470/93‑1 (MQ=255)
                                                                             gtgtGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTGCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAtt            <  1:7830641/90‑1 (MQ=255)
                                                                             gTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAtt            >  2:2075127/1‑93 (MQ=255)
                                                                                 gcgcGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTc        <  2:3285531/93‑1 (MQ=255)
                                                                                  cgcgACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCa       <  2:513297/93‑1 (MQ=255)
                                                                                  cgcgACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCa       <  1:5860302/93‑1 (MQ=255)
                                                                                  cgcgACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCa       <  1:9177517/93‑1 (MQ=255)
                                                                                   gcgACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATTTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCaa      >  2:929033/1‑92 (MQ=255)
                                                                                    cgACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATa     >  1:3326800/1‑93 (MQ=255)
                                                                                    cgACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATa     >  1:1192437/1‑93 (MQ=255)
                                                                                      ccTGCATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATAcc   <  2:4991820/92‑1 (MQ=255)
                                                                                      aCTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATAcc   <  1:9735172/93‑1 (MQ=255)
                                                                                      aCTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATAcc   <  2:4663734/93‑1 (MQ=255)
                                                                                      aCTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATAcc   <  2:5802981/93‑1 (MQ=255)
                                                                                       cTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCt  <  2:2400955/93‑1 (MQ=255)
                                                                                       cTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCt  >  1:4144076/1‑93 (MQ=255)
                                                                                       cTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCt  >  1:1006188/1‑93 (MQ=255)
                                                                                          |                                                                                         
TTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCT  >  NC_000913/2488291‑2488470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCA  >  NC_000913/2488288‑2488474
                                                                                             |                                                                                             
GACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGGAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGG                                                                                               <  SRR4302540.116492/94‑1 (MQ=60)
GACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGG                                                                                               <  SRR4302540.5777057/94‑1 (MQ=60)
 ACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGA                                                                                              <  SRR4302540.5070651/94‑1 (MQ=60)
 ACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGA                                                                                              >  SRR4302540.585570/1‑94 (MQ=60)
 ACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGA                                                                                              <  SRR4302540.9596650/94‑1 (MQ=60)
  CTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGAT                                                                                             <  SRR4302540.1735682/94‑1 (MQ=60)
   TTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATA                                                                                            <  SRR4302540.4721505/94‑1 (MQ=60)
   TTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATA                                                                                            <  SRR4302540.5963911/94‑1 (MQ=60)
   TTGTTTTGGGTTAAGTTTGGTAATATGGCCACTGTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATA                                                                                            <  SRR4302540.9809156/94‑1 (MQ=60)
    TGTTTTGGGTTAAGTTTGGGAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATAC                                                                                           >  SRR4302540.826795/1‑94 (MQ=60)
     GTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACC                                                                                          >  SRR4302540.6055999/1‑94 (MQ=60)
     GTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACC                                                                                          >  SRR4302540.6056000/1‑94 (MQ=60)
       TTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGG                                                                                        >  SRR4302540.3301270/1‑94 (MQ=60)
       TTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGG                                                                                        <  SRR4302540.3221511/94‑1 (MQ=60)
       TTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGG                                                                                        <  SRR4302540.4537376/94‑1 (MQ=60)
           GGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGT                                                                                    <  SRR4302540.3534532/94‑1 (MQ=60)
            GTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTT                                                                                   >  SRR4302540.3342719/1‑94 (MQ=60)
             TTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTA                                                                                  <  SRR4302540.2657040/94‑1 (MQ=60)
              TAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAA                                                                                 <  SRR4302540.7068051/94‑1 (MQ=60)
               AAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACTACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGGGGTTAAA                                                                                >  SRR4302540.1319412/1‑94 (MQ=60)
               AAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAA                                                                                <  SRR4302540.8953380/94‑1 (MQ=60)
               AAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAA                                                                                <  SRR4302540.4762712/94‑1 (MQ=60)
                AGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAG                                                                               >  SRR4302540.709403/1‑94 (MQ=60)
                AGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAG                                                                               >  SRR4302540.709402/1‑94 (MQ=60)
                AGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAG                                                                               <  SRR4302540.8697602/94‑1 (MQ=60)
                AGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAG                                                                               <  SRR4302540.7213089/94‑1 (MQ=60)
                  TTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCA                                                                             >  SRR4302540.6409481/1‑94 (MQ=60)
                  TTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCA                                                                             <  SRR4302540.7676450/94‑1 (MQ=60)
                   TTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCAT                                                                            <  SRR4302540.6583666/94‑1 (MQ=60)
                   TTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCAT                                                                            >  SRR4302540.69340/1‑94 (MQ=60)
                    TGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATG                                                                           >  SRR4302540.4904957/1‑94 (MQ=60)
                    TGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATG                                                                           <  SRR4302540.7496518/94‑1 (MQ=60)
                    TGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATG                                                                           >  SRR4302540.6484222/1‑94 (MQ=60)
                     TGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGA                                                                          <  SRR4302540.6419461/94‑1 (MQ=60)
                      GTAATATGGCCACGTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGAC                                                                         <  SRR4302540.6058974/94‑1 (MQ=60)
                       TAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACG                                                                        <  SRR4302540.8681960/94‑1 (MQ=60)
                       TAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACG                                                                        >  SRR4302540.3601477/1‑94 (MQ=60)
                       TAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACG                                                                        <  SRR4302540.4559360/94‑1 (MQ=60)
                        AATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGA                                                                       >  SRR4302540.9417774/1‑94 (MQ=60)
                        AATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGA                                                                       <  SRR4302540.3230456/94‑1 (MQ=60)
                        AATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGA                                                                       >  SRR4302540.4264746/1‑94 (MQ=60)
                         ATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAA                                                                      >  SRR4302540.3388922/1‑94 (MQ=60)
                         ATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAA                                                                      >  SRR4302540.1309271/1‑94 (MQ=60)
                          TATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAG                                                                     <  SRR4302540.8929878/94‑1 (MQ=60)
                          TATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAG                                                                     <  SRR4302540.1745308/94‑1 (MQ=60)
                          TATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAG                                                                     <  SRR4302540.8493260/94‑1 (MQ=60)
                          TATGGCCACTTTCAGTTCCTGCTGCAGGGTCGAGGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAG                                                                     <  SRR4302540.9825963/94‑1 (MQ=60)
                           ATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGT                                                                    >  SRR4302540.235051/1‑94 (MQ=60)
                             GGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTC                                                                  <  SRR4302540.8577161/94‑1 (MQ=60)
                               CCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCAT                                                                <  SRR4302540.3609883/94‑1 (MQ=60)
                                CACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATC                                                               >  SRR4302540.9812773/1‑94 (MQ=60)
                                CACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATC                                                               >  SRR4302540.6479549/1‑94 (MQ=60)
                                CACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATC                                                               >  SRR4302540.8263657/1‑94 (MQ=60)
                                CACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGGTCATT                                                               >  SRR4302540.9274127/1‑94 (MQ=60)
                                 ACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCT                                                              <  SRR4302540.4130283/94‑1 (MQ=60)
                                 ACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCT                                                              <  SRR4302540.4036114/94‑1 (MQ=60)
                                  CTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTG                                                             >  SRR4302540.1317711/1‑94 (MQ=60)
                                   TTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGT                                                            <  SRR4302540.5168900/94‑1 (MQ=60)
                                   TTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGT                                                            >  SRR4302540.872252/1‑94 (MQ=60)
                                   TTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGT                                                            <  SRR4302540.7073624/94‑1 (MQ=60)
                                    TTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTC                                                           <  SRR4302540.1935838/94‑1 (MQ=60)
                                    TTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTC                                                           <  SRR4302540.3609242/94‑1 (MQ=60)
                                    TTCAGTTCCTGCTGCAGGGTCCATGACCACATTAAGAATGGTCTGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTC                                                           <  SRR4302540.3609243/94‑1 (MQ=60)
                                     TCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCG                                                          <  SRR4302540.8413332/94‑1 (MQ=60)
                                     TCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCG                                                          <  SRR4302540.2496804/94‑1 (MQ=60)
                                     TCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGGGGTTAAAGCATGACGAAGTTCATCTGTCG                                                          >  SRR4302540.4985202/1‑94 (MQ=60)
                                       AGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTG                                                        >  SRR4302540.3250164/1‑94 (MQ=60)
                                        GTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACAAAGTTCATCTGTCGTGG                                                       >  SRR4302540.71716/1‑94 (MQ=60)
                                         TTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGT                                                      >  SRR4302540.8375594/1‑94 (MQ=60)
                                          TCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTG                                                     <  SRR4302540.5180069/94‑1 (MQ=60)
                                          TCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTG                                                     <  SRR4302540.6492719/94‑1 (MQ=60)
                                           CCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGA                                                    <  SRR4302540.1910982/94‑1 (MQ=60)
                                            CTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGAC                                                   >  SRR4302540.8762898/1‑94 (MQ=60)
                                            CTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGAC                                                   >  SRR4302540.130349/1‑94 (MQ=60)
                                            CTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGAC                                                   >  SRR4302540.130348/1‑94 (MQ=60)
                                            CTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGAC                                                   <  SRR4302540.801988/94‑1 (MQ=60)
                                             TGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACG                                                  >  SRR4302540.2267959/1‑94 (MQ=60)
                                                TGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTA                                               >  SRR4302540.8848381/1‑94 (MQ=60)
                                                TGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTA                                               >  SRR4302540.1070808/1‑94 (MQ=60)
                                                 GCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTAT                                              <  SRR4302540.10036695/94‑1 (MQ=60)
                                                  CAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATA                                             <  SRR4302540.3190036/94‑1 (MQ=60)
                                                   AGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAC                                            >  SRR4302540.6803400/1‑94 (MQ=60)
                                                    GGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGC                                           <  SRR4302540.2603793/94‑1 (MQ=60)
                                                     GGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCC                                          <  SRR4302540.4285961/94‑1 (MQ=60)
                                                      GTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCA                                         >  SRR4302540.1294980/1‑94 (MQ=60)
                                                      GTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCA                                         <  SRR4302540.3013926/94‑1 (MQ=60)
                                                       TCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGAATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAA                                        <  SRR4302540.5756797/94‑1 (MQ=60)
                                                       GCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAA                                        <  SRR4302540.514771/94‑1 (MQ=60)
                                                        CGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAAC                                       <  SRR4302540.878358/94‑1 (MQ=60)
                                                        CGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAAC                                       <  SRR4302540.4354832/94‑1 (MQ=60)
                                                         GATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACG                                      >  SRR4302540.3173362/1‑94 (MQ=60)
                                                         CATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACG                                      <  SRR4302540.3016931/94‑1 (MQ=60)
                                                         CATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACG                                      <  SRR4302540.3299843/94‑1 (MQ=60)
                                                          ATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGC                                     <  SRR4302540.1059543/94‑1 (MQ=60)
                                                          ATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGC                                     <  SRR4302540.7969690/94‑1 (MQ=60)
                                                           TGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCC                                    >  SRR4302540.4646082/1‑94 (MQ=60)
                                                           TGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCC                                    >  SRR4302540.34860/1‑94 (MQ=60)
                                                             ACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCAC                                  <  SRR4302540.5677614/94‑1 (MQ=60)
                                                             ACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCAC                                  >  SRR4302540.7150982/1‑94 (MQ=60)
                                                                ACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAA                               >  SRR4302540.2040346/1‑94 (MQ=60)
                                                                  ATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAAC                             <  SRR4302540.346761/94‑1 (MQ=60)
                                                                  ATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAAC                             >  SRR4302540.9345134/1‑94 (MQ=60)
                                                                  ATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAAC                             <  SRR4302540.6027318/94‑1 (MQ=60)
                                                                  ATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAAC                             <  SRR4302540.944116/94‑1 (MQ=60)
                                                                  ATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAAC                             <  SRR4302540.1114053/94‑1 (MQ=60)
                                                                  ATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAAC                             >  SRR4302540.6594872/1‑94 (MQ=60)
                                                                  ATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAAC                             <  SRR4302540.1419390/94‑1 (MQ=60)
                                                                  ATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAAC                             <  SRR4302540.2608360/94‑1 (MQ=60)
                                                                  ATTAATAATGGTCGGTGTGCGCGTCTGGATACCGGTGGTTAAAGCATGACGAAGTGCATCTGTCGTAGTGACGTTATAGCCAACGCCACGAAAC                             <  SRR4302540.818870/94‑1 (MQ=60)
                                                                   TTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACG                            <  SRR4302540.1278983/94‑1 (MQ=60)
                                                                    TAATAATGGTCGTGTTGCACGACGGGAGACCCGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGC                           <  SRR4302540.6983942/94‑1 (MQ=60)
                                                                    TAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGC                           <  SRR4302540.2834598/94‑1 (MQ=60)
                                                                     AATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCA                          >  SRR4302540.3438783/1‑94 (MQ=60)
                                                                     AATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCA                          >  SRR4302540.9650816/1‑94 (MQ=60)
                                                                     AATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCA                          >  SRR4302540.9650817/1‑94 (MQ=60)
                                                                      ATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGGAACGCAT                         >  SRR4302540.2154219/1‑94 (MQ=60)
                                                                      ATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCAT                         >  SRR4302540.9655496/1‑94 (MQ=60)
                                                                      ATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCAT                         <  SRR4302540.8079901/94‑1 (MQ=60)
                                                                      ATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCAT                         <  SRR4302540.6800709/94‑1 (MQ=60)
                                                                      ATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCAC                         >  SRR4302540.2154218/1‑94 (MQ=60)
                                                                       TAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATC                        <  SRR4302540.3456565/94‑1 (MQ=60)
                                                                        AATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCC                       >  SRR4302540.2438370/1‑94 (MQ=60)
                                                                        AATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCC                       <  SRR4302540.7738268/94‑1 (MQ=60)
                                                                        AATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCC                       <  SRR4302540.4691825/94‑1 (MQ=60)
                                                                         ATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCA                      >  SRR4302540.2681854/1‑94 (MQ=60)
                                                                         ATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCA                      <  SRR4302540.6521963/94‑1 (MQ=60)
                                                                          TGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCAT                     <  SRR4302540.5678884/94‑1 (MQ=60)
                                                                           GGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATT                    <  SRR4302540.5772601/94‑1 (MQ=60)
                                                                            GTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTA                   >  SRR4302540.7261895/1‑94 (MQ=60)
                                                                             TCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAA                  <  SRR4302540.7409479/94‑1 (MQ=60)
                                                                             TCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAA                  <  SRR4302540.5875503/94‑1 (MQ=60)
                                                                              CGGTTTGCGCGACTGGATACCGTTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAT                 >  SRR4302540.1789335/1‑94 (MQ=60)
                                                                              CGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAT                 <  SRR4302540.10029933/94‑1 (MQ=60)
                                                                              CGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAT                 <  SRR4302540.8125148/94‑1 (MQ=60)
                                                                              CGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAT                 <  SRR4302540.2285732/94‑1 (MQ=60)
                                                                              CGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAT                 <  SRR4302540.4110849/94‑1 (MQ=60)
                                                                               GGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATT                >  SRR4302540.3365263/1‑94 (MQ=60)
                                                                                GTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTT               >  SRR4302540.7701392/1‑94 (MQ=60)
                                                                                GTGTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTGCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTT               <  SRR4302540.7922138/94‑1 (MQ=60)
                                                                                 TTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTG              <  SRR4302540.4569434/94‑1 (MQ=60)
                                                                                  TTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGT             <  SRR4302540.4880929/94‑1 (MQ=60)
                                                                                    GCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCA           <  SRR4302540.1063375/94‑1 (MQ=60)
                                                                                     CGCGACTGGATCCCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCAT          <  SRR4302540.3217149/94‑1 (MQ=60)
                                                                                     CGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCCTTCATTTGTCAA          >  SRR4302540.8919524/1‑94 (MQ=60)
                                                                                     CGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCAT          <  SRR4302540.10000080/94‑1 (MQ=60)
                                                                                     CGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCAT          >  SRR4302540.8919523/1‑94 (MQ=60)
                                                                                     CGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCAT          <  SRR4302540.5925110/94‑1 (MQ=60)
                                                                                     CGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCAT          <  SRR4302540.7589026/94‑1 (MQ=60)
                                                                                     CGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCAT          <  SRR4302540.9287590/94‑1 (MQ=60)
                                                                                     CGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCAT          <  SRR4302540.3217150/94‑1 (MQ=60)
                                                                                     CGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCAT          <  SRR4302540.6761826/94‑1 (MQ=60)
                                                                                     CGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGGGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCAT          >  SRR4302540.1895200/1‑94 (MQ=60)
                                                                                      GCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATA         >  SRR4302540.1204726/1‑94 (MQ=60)
                                                                                      GCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATA         >  SRR4302540.3363309/1‑94 (MQ=60)
                                                                                       CGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATAC        <  SRR4302540.4237591/94‑1 (MQ=60)
                                                                                       CGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATAC        <  SRR4302540.4237590/94‑1 (MQ=60)
                                                                                       CGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATAC        <  SRR4302540.3132915/94‑1 (MQ=60)
                                                                                         ACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCT      <  SRR4302540.9849478/94‑1 (MQ=60)
                                                                                         ACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCT      >  SRR4302540.1016392/1‑94 (MQ=60)
                                                                                         ACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCT      >  SRR4302540.4188924/1‑94 (MQ=60)
                                                                                          CTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTT     <  SRR4302540.6889139/94‑1 (MQ=60)
                                                                                          CTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTT     <  SRR4302540.1144406/94‑1 (MQ=60)
                                                                                          CTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTT     <  SRR4302540.8433644/94‑1 (MQ=60)
                                                                                          CTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTT     <  SRR4302540.4808867/94‑1 (MQ=60)
                                                                                            GGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGC   >  SRR4302540.593273/1‑94 (MQ=60)
                                                                                             GATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCA  <  SRR4302540.4320120/94‑1 (MQ=60)
                                                                                             |                                                                                             
GACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGCA  >  NC_000913/2488288‑2488474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑