Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,488,381 A→G I530I (ATT→ATC)  b2373 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,488,3810AG100.0% 523.8 / NA 160I530I (ATT→ATC) b2373hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (59/101);  total (59/101)

Only 100 of 165 total aligned reads displayed.

TTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCT  >  NC_000913/2488291‑2488470
                                                                                          |                                                                                         
ttGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGAt                                                                                         <  2:3071552/93‑1 (MQ=255)
ttGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGAt                                                                                         <  2:4791697/93‑1 (MQ=255)
ttGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGAt                                                                                         <  1:1962170/93‑1 (MQ=255)
  gTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCAAATTAATAATGGTCGGTTTGCGCGACTGGATAc                                                                                       <  1:58110/93‑1 (MQ=255)
  gTTTTGGGCTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATAc                                                                                       >  1:668153/1‑93 (MQ=255)
   ttttGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATAcc                                                                                      <  2:910125/93‑1 (MQ=255)
   ttttGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATAcc                                                                                      >  1:2056618/1‑93 (MQ=255)
     ttGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCgg                                                                                    >  1:453575/1‑93 (MQ=255)
     ttGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCgg                                                                                    >  1:5038897/1‑93 (MQ=255)
      tGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCggt                                                                                   >  2:5800062/1‑93 (MQ=255)
      tGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCggt                                                                                   <  1:3320555/93‑1 (MQ=255)
         gTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCggtggt                                                                                >  1:4448264/1‑93 (MQ=255)
          ttAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGtt                                                                               <  1:3408974/93‑1 (MQ=255)
          ttAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGtt                                                                               <  2:1068963/93‑1 (MQ=255)
             aGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTaaa                                                                            >  2:5690747/1‑93 (MQ=255)
               tttGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGc                                                                          <  2:2834207/93‑1 (MQ=255)
               tttGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGc                                                                          <  2:3322105/93‑1 (MQ=255)
                ttGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCa                                                                         <  2:6285498/93‑1 (MQ=255)
                 tgctAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCAt                                                                        <  1:1438503/90‑1 (MQ=255)
                 tGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCAt                                                                        <  1:1548492/93‑1 (MQ=255)
                  ggTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATg                                                                       <  2:2428846/93‑1 (MQ=255)
                  ggTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATg                                                                       <  2:5316905/93‑1 (MQ=255)
                   gTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGa                                                                      >  2:291889/1‑93 (MQ=255)
                   gTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGa                                                                      >  2:1109193/1‑93 (MQ=255)
                   gTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGa                                                                      <  1:3658263/93‑1 (MQ=255)
                     aaTATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACg                                                                    >  2:5954403/1‑93 (MQ=255)
                     aaTATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACg                                                                    <  1:3508620/93‑1 (MQ=255)
                      atatGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGa                                                                   <  1:1078468/93‑1 (MQ=255)
                      atatGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGa                                                                   <  2:119544/93‑1 (MQ=255)
                      atatGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGATTGCGCGACTGGATACCGGTGGTTAAAGCATGACGa                                                                   >  2:981171/1‑93 (MQ=255)
                       tatGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGaa                                                                  >  2:6068785/1‑93 (MQ=255)
                       tatGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGaa                                                                  <  2:3897959/93‑1 (MQ=255)
                        atGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAg                                                                 <  1:5098920/93‑1 (MQ=255)
                        atGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAg                                                                 <  2:453575/93‑1 (MQ=255)
                        atGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAg                                                                 <  1:810733/93‑1 (MQ=255)
                         tGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGt                                                                <  1:4314938/93‑1 (MQ=255)
                           gCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACNAAGNTc                                                              >  1:1531777/1‑93 (MQ=255)
                            ccACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCa                                                             <  2:726089/93‑1 (MQ=255)
                             cACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCAt                                                            >  2:5172283/1‑93 (MQ=255)
                             cACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCAt                                                            <  1:438650/93‑1 (MQ=255)
                             cACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCAt                                                            <  1:3454382/93‑1 (MQ=255)
                              aCTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATc                                                           <  2:2016349/93‑1 (MQ=255)
                              aCTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATc                                                           >  1:607321/1‑93 (MQ=255)
                                  tCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTc                                                       <  2:2583289/93‑1 (MQ=255)
                                   cAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCg                                                      <  2:4526855/93‑1 (MQ=255)
                                   cAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCg                                                      >  1:3953432/1‑93 (MQ=255)
                                    aGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgt                                                     >  1:4520937/1‑93 (MQ=255)
                                    aGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgt                                                     <  2:3281532/93‑1 (MQ=255)
                                    aGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgt                                                     >  2:1895037/1‑93 (MQ=255)
                                     gTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgtg                                                    >  2:504695/1‑93 (MQ=255)
                                      ttCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgtgg                                                   >  2:663935/1‑93 (MQ=255)
                                       tCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCgtggt                                                  <  2:3540049/93‑1 (MQ=255)
                                          tgctgcAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGAc                                               >  1:5362519/1‑93 (MQ=255)
                                            ctgcAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGt                                             <  1:1002548/93‑1 (MQ=255)
                                             tgcAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGtt                                            <  1:597207/93‑1 (MQ=255)
                                               cAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTtat                                          <  1:1835357/93‑1 (MQ=255)
                                               cAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTtat                                          <  1:4988144/93‑1 (MQ=255)
                                                aGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTtata                                         <  1:1682493/93‑1 (MQ=255)
                                                   gTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGcc                                      >  1:735226/1‑93 (MQ=255)
                                                     cGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCaa                                    <  1:609762/93‑1 (MQ=255)
                                                     cGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCaa                                    >  1:2191786/1‑93 (MQ=255)
                                                      gATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAAc                                   <  2:4348966/93‑1 (MQ=255)
                                                        tGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGc                                 >  2:1052089/1‑93 (MQ=255)
                                                        tGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGc                                 <  2:207229/93‑1 (MQ=255)
                                                        tGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGc                                 >  2:558039/1‑93 (MQ=255)
                                                          aCCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCa                               <  2:2742212/93‑1 (MQ=255)
                                                             acaTTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGa                            <  1:2458812/93‑1 (MQ=255)
                                                               aTTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGaaa                          >  1:730782/1‑93 (MQ=255)
                                                                ttAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAAc                         >  1:2895667/1‑93 (MQ=255)
                                                                ttAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAAc                         <  1:4075061/93‑1 (MQ=255)
                                                                 taataaTGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACg                        <  2:2710891/93‑1 (MQ=255)
                                                                  aataatGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGc                       >  1:5675500/1‑93 (MQ=255)
                                                                  aataatGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGc                       >  2:3425125/1‑93 (MQ=255)
                                                                   ataatGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCa                      >  1:1254392/1‑93 (MQ=255)
                                                                   ataatGGTCGGTTTGCGAGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCa                      <  2:4348806/93‑1 (MQ=255)
                                                                     aatGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGTCACGAAACGCATc                    <  2:6191533/93‑1 (MQ=255)
                                                                     aatGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATc                    <  1:2344823/93‑1 (MQ=255)
                                                                       tGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCa                  <  1:351250/93‑1 (MQ=255)
                                                                       tGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCa                  <  1:2724590/93‑1 (MQ=255)
                                                                         gTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCAtt                <  2:4456155/93‑1 (MQ=255)
                                                                         gTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCAtt                <  1:2029263/93‑1 (MQ=255)
                                                                         gTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCAtt                <  1:3159387/93‑1 (MQ=255)
                                                                           cGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTaa              <  2:3652888/93‑1 (MQ=255)
                                                                            ggTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAt             <  1:4625224/93‑1 (MQ=255)
                                                                            ggTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAt             <  2:2929064/93‑1 (MQ=255)
                                                                             gTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATTCATTAAtt            >  1:2309017/1‑93 (MQ=255)
                                                                             gTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAtt            >  2:2174613/1‑93 (MQ=255)
                                                                             gTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAtt            <  2:1917347/93‑1 (MQ=255)
                                                                             gTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAtt            >  1:1025950/1‑93 (MQ=255)
                                                                              tttGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAttt           <  1:1592871/93‑1 (MQ=255)
                                                                              tttGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAAttt           >  1:5512937/1‑93 (MQ=255)
                                                                              tttGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAAAGCCACGAAACGCATCCATTAAttt           >  2:1325691/1‑93 (MQ=255)
                                                                                  cgcgACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCa       <  1:2542948/93‑1 (MQ=255)
                                                                                   gcgACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCAt      <  2:5392489/93‑1 (MQ=255)
                                                                                   gcgACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCAt      <  2:242743/93‑1 (MQ=255)
                                                                                    cgACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCAtc     >  2:737985/1‑92 (MQ=255)
                                                                                    cgACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATa     <  2:4753060/93‑1 (MQ=255)
                                                                                     gACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATAc    >  2:1563737/1‑93 (MQ=255)
                                                                                      gcTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATAcc   <  1:3849506/92‑1 (MQ=255)
                                                                                      aCTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATAcc   <  1:2532500/93‑1 (MQ=255)
                                                                                          |                                                                                         
TTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCT  >  NC_000913/2488291‑2488470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGC  >  NC_000913/2488288‑2488473
                                                                                             |                                                                                            
GACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGG                                                                                              <  SRR4302533.1792268/94‑1 (MQ=60)
  CTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGAT                                                                                            <  SRR4302533.2746148/94‑1 (MQ=60)
  CTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGAT                                                                                            <  SRR4302533.2898257/94‑1 (MQ=60)
   TTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATA                                                                                           <  SRR4302533.1974147/94‑1 (MQ=60)
    TGTTTTGGGCTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATAC                                                                                          >  SRR4302533.672102/1‑94 (MQ=60)
     GTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACC                                                                                         >  SRR4302533.2069303/1‑94 (MQ=60)
     GTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCAAATTAATAATGGTCGGTTTGCGCGACTGGATACC                                                                                         <  SRR4302533.58495/94‑1 (MQ=60)
      TTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCG                                                                                        <  SRR4302533.133515/94‑1 (MQ=60)
       TTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGG                                                                                       >  SRR4302533.456286/1‑94 (MQ=60)
       TTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGG                                                                                       >  SRR4302533.5069406/1‑94 (MQ=60)
        TTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGT                                                                                      <  SRR4302533.1500/94‑1 (MQ=60)
         TGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTG                                                                                     <  SRR4302533.3341066/94‑1 (MQ=60)
           GGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGT                                                                                   >  SRR4302533.4475167/1‑94 (MQ=60)
           GGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGT                                                                                   <  SRR4302533.5557189/94‑1 (MQ=60)
             TTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTA                                                                                 <  SRR4302533.3430032/94‑1 (MQ=60)
                  TTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCA                                                                            <  SRR4302533.6041914/94‑1 (MQ=60)
                  TTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCCGTTTGCGCGACTGGATACCGGTGGATAAACCA                                                                            >  SRR4302533.3478242/1‑94 (MQ=60)
                    TGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATG                                                                          <  SRR4302533.1557823/94‑1 (MQ=60)
                    TGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATG                                                                          <  SRR4302533.2715268/94‑1 (MQ=60)
                    TGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGGTAAAGCATG                                                                          >  SRR4302533.620267/1‑94 (MQ=60)
                    TGCTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATG                                                                          <  SRR4302533.1447185/94‑1 (MQ=60)
                      GTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGAC                                                                        <  SRR4302533.3680757/94‑1 (MQ=60)
                        AATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGA                                                                      >  SRR4302533.3274194/1‑94 (MQ=60)
                        AATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGA                                                                      <  SRR4302533.3530252/94‑1 (MQ=60)
                         ATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAA                                                                     <  SRR4302533.1084823/94‑1 (MQ=60)
                           ATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGT                                                                   <  SRR4302533.815487/94‑1 (MQ=60)
                           ATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGT                                                                   <  SRR4302533.5129727/94‑1 (MQ=60)
                           ATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGT                                                                   <  SRR4302533.2396257/94‑1 (MQ=60)
                            TGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTT                                                                  <  SRR4302533.4340917/94‑1 (MQ=60)
                             GGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACNAAGNTC                                                                 >  SRR4302533.1540982/1‑94 (MQ=60)
                                CACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATC                                                              >  SRR4302533.610896/1‑94 (MQ=60)
                                CACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATC                                                              <  SRR4302533.441265/94‑1 (MQ=60)
                                CACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATC                                                              <  SRR4302533.3475705/94‑1 (MQ=60)
                                 ACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCT                                                             >  SRR4302533.4507471/1‑94 (MQ=60)
                                    TTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTC                                                          <  SRR4302533.4330857/94‑1 (MQ=60)
                                     TCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCG                                                         >  SRR4302533.3977537/1‑94 (MQ=60)
                                      CAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGT                                                        >  SRR4302533.4548311/1‑94 (MQ=60)
                                      CAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGT                                                        >  SRR4302533.1600846/1‑94 (MQ=60)
                                       AGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTG                                                       >  SRR4302533.1992120/1‑94 (MQ=60)
                                         TTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGT                                                     <  SRR4302533.806576/94‑1 (MQ=60)
                                            CTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGAC                                                  >  SRR4302533.5394726/1‑94 (MQ=60)
                                            CTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGAC                                                  >  SRR4302533.710020/1‑94 (MQ=60)
                                             TGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACG                                                 <  SRR4302533.2881150/94‑1 (MQ=60)
                                              GCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGT                                                <  SRR4302533.4571106/94‑1 (MQ=60)
                                               CTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTT                                               <  SRR4302533.1008401/94‑1 (MQ=60)
                                                TGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTA                                              <  SRR4302533.600729/94‑1 (MQ=60)
                                                  CAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATA                                            <  SRR4302533.1846556/94‑1 (MQ=60)
                                                  CAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATA                                            <  SRR4302533.1043074/94‑1 (MQ=60)
                                                  CAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATA                                            <  SRR4302533.5018389/94‑1 (MQ=60)
                                                   AGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAG                                           <  SRR4302533.227617/94‑1 (MQ=60)
                                                   AGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAG                                           >  SRR4302533.5197060/1‑94 (MQ=60)
                                                   AGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAG                                           <  SRR4302533.1692683/94‑1 (MQ=60)
                                                     GGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCC                                         >  SRR4302533.739551/1‑94 (MQ=60)
                                                       TCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAA                                       >  SRR4302533.2205292/1‑94 (MQ=60)
                                                        CGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAAC                                      <  SRR4302533.5896387/94‑1 (MQ=60)
                                                        CGATGACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAAC                                      <  SRR4302533.613348/94‑1 (MQ=60)
                                                             ACCACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCAC                                 <  SRR4302533.16151/94‑1 (MQ=60)
                                                                ACATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAA                              <  SRR4302533.2473984/94‑1 (MQ=60)
                                                                 CATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAA                             >  SRR4302533.735087/1‑94 (MQ=60)
                                                                  ATTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAAC                            >  SRR4302533.2913475/1‑94 (MQ=60)
                                                                   TTAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACG                           <  SRR4302533.4099869/94‑1 (MQ=60)
                                                                    TAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGC                          >  SRR4302533.5709778/1‑94 (MQ=60)
                                                                    TAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGC                          <  SRR4302533.3454541/94‑1 (MQ=60)
                                                                    TAATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGC                          >  SRR4302533.248970/1‑94 (MQ=60)
                                                                     AATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCA                         >  SRR4302533.1261893/1‑94 (MQ=60)
                                                                      ATAATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCAT                        <  SRR4302533.3826642/94‑1 (MQ=60)
                                                                        AATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCC                      <  SRR4302533.1884045/94‑1 (MQ=60)
                                                                        AATGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCC                      <  SRR4302533.2359334/94‑1 (MQ=60)
                                                                          TGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCAT                    <  SRR4302533.353392/94‑1 (MQ=60)
                                                                          TGGTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCAT                    <  SRR4302533.2741307/94‑1 (MQ=60)
                                                                            GTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATGCATTA                  <  SRR4302533.510247/94‑1 (MQ=60)
                                                                            GTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTA                  <  SRR4302533.2041788/94‑1 (MQ=60)
                                                                            GTCGGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTA                  <  SRR4302533.3178831/94‑1 (MQ=60)
                                                                               GGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATTCATTAATT               >  SRR4302533.2323322/1‑94 (MQ=60)
                                                                               GGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATT               <  SRR4302533.4653258/94‑1 (MQ=60)
                                                                               GGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATT               >  SRR4302533.1031979/1‑94 (MQ=60)
                                                                               GGTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATT               >  SRR4302533.4072863/1‑94 (MQ=60)
                                                                                GTTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTT              >  SRR4302533.5546216/1‑94 (MQ=60)
                                                                                 TTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTG             <  SRR4302533.3677852/94‑1 (MQ=60)
                                                                                 TTTGCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTG             <  SRR4302533.1602509/94‑1 (MQ=60)
                                                                                    GCGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCA          >  SRR4302533.4939274/1‑94 (MQ=60)
                                                                                     CGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCAT         <  SRR4302533.2558597/94‑1 (MQ=60)
                                                                                     CGCGACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCAT         <  SRR4302533.4430244/94‑1 (MQ=60)
                                                                                        GACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACC      <  SRR4302533.2111324/94‑1 (MQ=60)
                                                                                         ACTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCT     <  SRR4302533.2548089/94‑1 (MQ=60)
                                                                                          CTGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTT    >  SRR4302533.1234359/1‑94 (MQ=60)
                                                                                           TGGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTG   <  SRR4302533.2454277/94‑1 (MQ=60)
                                                                                            GGATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGC  >  SRR4302533.2455052/1‑94 (MQ=60)
                                                                                             |                                                                                            
GACTTGTTTTGGGTTAAGTTTGGTAATATGGCCACTTTCAGTTCCTGCTGCAGGGTCGATGACCACATTAATAATGGTCGGTTTGCGCGACTGAATACCGGTGGTTAAAGCATGACGAAGTTCATCTGTCGTGGTGACGTTATAGCCAACGCCACGAAACGCATCCATTAATTTGTCATACCTTGC  >  NC_000913/2488288‑2488473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑