Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,501,888 +C coding (618/2496 nt) b2383 ← putative PTS system enzyme IIA component, enzyme I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,501,8841.C99.0% 617.0 / ‑1.6 197coding (622/2496 nt)b2383putative PTS system enzyme IIA component, enzyme I
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base C (98/97);  total (100/97)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 235 total aligned reads displayed.

AATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑CC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGT  >  NC_000913/2501792‑2501971
                                                                                             |                                                                                        
aaTGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAg                                                                                           >  2:7412509/1‑93 (MQ=255)
 aTGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑c                                                                                         <  1:7038548/93‑1 (MQ=255)
 aTGTCGCTAACACGTTCGCGCAGATAAGCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑c                                                                                         <  1:6010949/93‑1 (MQ=255)
     cGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑cc‑ccc                                                                                    >  2:4619191/1‑92 (MQ=255)
     cGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑cc‑ccc                                                                                    >  1:2278184/1‑92 (MQ=255)
     cGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑cc‑ccc                                                                                    <  1:4786155/93‑2 (MQ=255)
      gCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑cc‑ccct                                                                                   <  2:6301005/93‑3 (MQ=255)
      gCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑cc‑ccct                                                                                   <  2:4935330/93‑3 (MQ=255)
       cTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑cc‑ccctg                                                                                  >  1:9106514/1‑90 (MQ=255)
        tAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑cc‑ccctga                                                                                 <  1:5171570/93‑5 (MQ=255)
        tAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑cc‑ccctga                                                                                 >  2:5319302/1‑89 (MQ=255)
        tAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑cc‑ccctga                                                                                 >  2:2320157/1‑89 (MQ=255)
        tAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑cc‑ccctga                                                                                 >  2:8377080/1‑89 (MQ=255)
          acacGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATg                                                                                >  2:282701/1‑93 (MQ=255)
          acacGTTCGCGCAAATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATg                                                                                <  1:6498179/93‑1 (MQ=255)
               ttCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGtt                                                                           >  2:8479189/1‑93 (MQ=255)
               ttCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGtt                                                                           >  1:2849140/1‑93 (MQ=255)
                   cgcAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGt                                                                       >  2:9114632/1‑93 (MQ=255)
                    gcAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGtc                                                                      <  2:7303610/93‑1 (MQ=255)
                      aGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGtcat                                                                    <  1:8511953/93‑1 (MQ=255)
                      aGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGtcat                                                                    <  1:1702453/93‑1 (MQ=255)
                      aGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGtcat                                                                    <  2:9013052/93‑1 (MQ=255)
                       gATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGtcatc                                                                   >  2:1206754/1‑93 (MQ=255)
                       gATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGtcatc                                                                   >  2:6534689/1‑93 (MQ=255)
                        aTAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGtcatca                                                                  >  1:8090432/1‑93 (MQ=255)
                           aTCGCTGGCATAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGc                                                               >  1:9886678/1‑93 (MQ=255)
                           aTCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGc                                                               >  1:5939571/1‑93 (MQ=255)
                            tCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCg                                                              <  1:2385786/93‑1 (MQ=255)
                            tCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCg                                                              >  2:1347028/1‑93 (MQ=255)
                             cGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGa                                                             <  2:1967155/93‑1 (MQ=255)
                              gCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGAc                                                            <  1:7299758/93‑1 (MQ=255)
                              gCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGAc                                                            >  2:5841392/1‑93 (MQ=255)
                              gCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGAc                                                            >  1:4362581/1‑93 (MQ=255)
                              gCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGAc                                                            >  1:3103724/1‑93 (MQ=255)
                                tGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACgg                                                          >  2:3571007/1‑93 (MQ=255)
                                tGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACgg                                                          >  2:4313211/1‑93 (MQ=255)
                                tGGCAGAGGCAGAAAGTTTGGAGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACgg                                                          >  1:9514901/1‑93 (MQ=255)
                                 ggcagaggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑CCACCTGATGCTGTTCTGTCATCAGGCGACGGa                                                         >  2:897139/1‑93 (MQ=255)
                                  gcagaggcagaAAGTTTGGCCCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGat                                                        <  2:2691541/93‑1 (MQ=255)
                                   cagaggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCTCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGata                                                       <  1:4091546/93‑1 (MQ=255)
                                   cagaggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGata                                                       <  2:6247052/93‑1 (MQ=255)
                                    agaggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGatat                                                      <  1:365884/93‑1 (MQ=255)
                                     gaggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATAtt                                                     >  2:872147/1‑93 (MQ=255)
                                     gaggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATAtt                                                     >  2:7237627/1‑93 (MQ=255)
                                       ggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGc                                                   <  2:6243220/93‑1 (MQ=255)
                                       ggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGc                                                   >  2:6351132/1‑93 (MQ=255)
                                        gcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGcc                                                  >  2:1422710/1‑93 (MQ=255)
                                         cagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCt                                                 <  2:5594426/93‑1 (MQ=255)
                                         cagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTAATCAGGCGACGGATATTGCCt                                                 <  2:6025519/93‑1 (MQ=255)
                                          agaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTg                                                >  2:8206989/1‑93 (MQ=255)
                                          agaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTg                                                >  1:1515242/1‑93 (MQ=255)
                                           gaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGc                                               <  1:5519027/93‑1 (MQ=255)
                                               gTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAAt                                           >  2:2912988/1‑93 (MQ=255)
                                               gTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCACGCGACGGATATTGACTGCAAAt                                           >  2:1107497/1‑93 (MQ=255)
                                                tttGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAAtt                                          >  1:4856642/1‑93 (MQ=255)
                                                gttcgcgcAAACCTGCTTCATATTGCTGATGATCGCCCTCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAAtt                                          <  1:6735028/89‑1 (MQ=255)
                                                  tGGCGCAAACCTGCTTCATATTGCTGAGGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCCGGCAACAGGCGACGGATATTGCCTGCAAATtca                                        <  2:5262125/93‑1 (MQ=255)
                                                  tGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATtca                                        <  2:6680411/93‑1 (MQ=255)
                                                   ggCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATtcat                                       <  1:4811240/93‑1 (MQ=255)
                                                   ggCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATtcat                                       <  1:10023951/93‑1 (MQ=255)
                                                     cgcAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATtcatca                                     <  1:1021307/93‑1 (MQ=255)
                                                     cgcAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATtcatca                                     <  2:6796460/93‑1 (MQ=255)
                                                     cgcAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATtcatca                                     <  1:9553160/93‑1 (MQ=255)
                                                      gcAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTcatcat                                    <  1:6166676/93‑1 (MQ=255)
                                                      gcAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTcatcat                                    >  2:939521/1‑93 (MQ=255)
                                                       cAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCatcatc                                   >  2:904791/1‑93 (MQ=255)
                                                       cAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGAGACGGATATTGCCTGCAAATTCatcatc                                   >  1:2470532/1‑93 (MQ=255)
                                                        aaaCCTGCTCCATATTGCTGATGATCGCCTCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATcc                                  <  1:9783346/93‑1 (MQ=255)
                                                        aaaCCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATcc                                  <  2:3824226/93‑1 (MQ=255)
                                                        aaaCCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATcc                                  >  2:1649865/1‑93 (MQ=255)
                                                         aaCCTGCTCCATATTGCTGATTGTCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCt                                 >  1:1832866/1‑93 (MQ=255)
                                                         aaCCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCt                                 >  2:3307517/1‑93 (MQ=255)
                                                         aaCCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCt                                 >  2:5838339/1‑93 (MQ=255)
                                                          aCCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTg                                >  1:8433528/1‑93 (MQ=255)
                                                           ccTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGa                               <  1:7400396/93‑1 (MQ=255)
                                                            cTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGaa                              >  2:9328715/1‑93 (MQ=255)
                                                               cTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCa                           >  1:3199999/1‑93 (MQ=255)
                                                               cTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCa                           <  1:4607174/93‑1 (MQ=255)
                                                               cTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCa                           <  1:6621388/93‑1 (MQ=255)
                                                                 ccATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGc                         <  1:9346670/93‑1 (MQ=255)
                                                                   atatTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGa                       >  2:3502603/1‑93 (MQ=255)
                                                                    tatTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGAc                      >  2:3855378/1‑93 (MQ=255)
                                                                     atTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACa                     <  2:9729345/93‑1 (MQ=255)
                                                                       tGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACaaa                   <  1:5422930/93‑1 (MQ=255)
                                                                        gCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAAt                  <  1:7584181/93‑1 (MQ=255)
                                                                         cTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATg                 <  1:3358009/93‑1 (MQ=255)
                                                                         cTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATg                 <  2:5713459/93‑1 (MQ=255)
                                                                           gatgatCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATggg               >  2:4057395/1‑93 (MQ=255)
                                                                            atgatCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGgc              <  2:2248395/93‑1 (MQ=255)
                                                                              gatCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGgcgc            <  1:714673/93‑1 (MQ=255)
                                                                              gatCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGgcgc            >  1:2974750/1‑93 (MQ=255)
                                                                              gatCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGgcgc            >  1:2001473/1‑93 (MQ=255)
                                                                              gatCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGgcgc            <  2:145941/93‑1 (MQ=255)
                                                                                tCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTg          >  1:4450479/1‑93 (MQ=255)
                                                                                tCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTg          <  2:7938681/93‑1 (MQ=255)
                                                                                  gccgccCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGGGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAg        >  1:2815735/1‑93 (MQ=255)
                                                                                  gccgccCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAg        <  1:8377081/93‑1 (MQ=255)
                                                                                       ccccAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAg   <  1:8293147/93‑1 (MQ=255)
                                                                                       ccccAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAg   <  2:8102287/93‑1 (MQ=255)
                                                                                        cccAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGt  >  2:9179703/1‑93 (MQ=255)
                                                                                             |                                                                                        
AATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑CC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGT  >  NC_000913/2501792‑2501971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑C‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCG  >  NC_000913/2501795‑2501981
                                                                                               |                                                                                             
GTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑C‑CCC                                                                                               >  SRR4302540.6644034/1‑94 (MQ=60)
 TCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑C‑CCCC                                                                                              >  SRR4302540.7618886/1‑94 (MQ=60)
 TCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑C‑CCCC                                                                                              >  SRR4302540.2302652/1‑94 (MQ=60)
  CGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑C‑CCCct                                                                                             >  SRR4302540.9986350/1‑92 (MQ=60)
  CGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑C‑CCCct                                                                                             >  SRR4302540.5801039/1‑92 (MQ=60)
  CGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑C‑CCCct                                                                                             <  SRR4302540.4837325/94‑3 (MQ=60)
  CACTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑C‑CCCct                                                                                             >  SRR4302540.2435203/1‑92 (MQ=60)
   GCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑C‑CCCctg                                                                                            >  SRR4302540.9215950/1‑91 (MQ=60)
    CTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGA                                                                                            <  SRR4302540.3000711/94‑1 (MQ=60)
     TAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGAT                                                                                           <  SRR4302540.6737533/94‑1 (MQ=60)
     TAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGAT                                                                                           <  SRR4302540.5227850/94‑1 (MQ=60)
      AACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATG                                                                                          >  SRR4302540.4195131/1‑94 (MQ=60)
      AACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATG                                                                                          >  SRR4302540.4463395/1‑94 (MQ=60)
       ACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGC                                                                                         <  SRR4302540.6570427/94‑1 (MQ=60)
       ACACGTTCGCGCAAATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGC                                                                                         <  SRR4302540.6570428/94‑1 (MQ=60)
        CACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCT                                                                                        >  SRR4302540.6138546/1‑94 (MQ=60)
         ACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTG                                                                                       <  SRR4302540.455750/94‑1 (MQ=60)
         ACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTG                                                                                       >  SRR4302540.2708713/1‑94 (MQ=60)
           GTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTT                                                                                     >  SRR4302540.6320042/1‑94 (MQ=60)
           GTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTT                                                                                     >  SRR4302540.8460554/1‑94 (MQ=60)
           GTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTT                                                                                     >  SRR4302540.2879998/1‑94 (MQ=60)
                   AGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATC                                                                             <  SRR4302540.1720879/94‑1 (MQ=60)
                   AGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATC                                                                             <  SRR4302540.8610879/94‑1 (MQ=60)
                    GATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCA                                                                            >  SRR4302540.8184337/1‑94 (MQ=60)
                     ATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCTCCCAGCC‑CCCTGATGCTGTTCTGTCATCAG                                                                           <  SRR4302540.1607558/94‑1 (MQ=60)
                     ATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCCGCC‑CCCTGATGCTGTTCTGTCATCAG                                                                           >  SRR4302540.7083737/1‑94 (MQ=60)
                     ATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAG                                                                           <  SRR4302540.359451/94‑1 (MQ=60)
                      TAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGG                                                                          >  SRR4302540.2012545/1‑94 (MQ=60)
                       AATCGCTGGCATAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGC                                                                         >  SRR4302540.10002121/1‑94 (MQ=60)
                       AATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGC                                                                         >  SRR4302540.6005230/1‑94 (MQ=60)
                       AATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGCTCTGTCATCAGGC                                                                         >  SRR4302540.6005532/1‑94 (MQ=60)
                        ATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCG                                                                        >  SRR4302540.2389899/1‑94 (MQ=60)
                         TCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGA                                                                       >  SRR4302540.7038861/1‑94 (MQ=60)
                         TCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGA                                                                       <  SRR4302540.2411496/94‑1 (MQ=60)
                          CGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGAC                                                                      >  SRR4302540.3137708/1‑94 (MQ=60)
                          CGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGAC                                                                      >  SRR4302540.4410009/1‑94 (MQ=60)
                           GCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACG                                                                     <  SRR4302540.7381240/94‑1 (MQ=60)
                            CTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGG                                                                    <  SRR4302540.8570951/94‑1 (MQ=60)
                            CTGGCAGAGGCAGAAAGTTTGGAGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGG                                                                    >  SRR4302540.9627598/1‑94 (MQ=60)
                              GGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGAT                                                                  <  SRR4302540.8271540/94‑1 (MQ=60)
                              GGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGAT                                                                  >  SRR4302540.443664/1‑94 (MQ=60)
                                CAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCTCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATAT                                                                <  SRR4302540.4135687/94‑1 (MQ=60)
                                CAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATAT                                                                >  SRR4302540.3368259/1‑94 (MQ=60)
                                 AGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCTCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATT                                                               <  SRR4302540.3703169/94‑1 (MQ=60)
                                 AGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATT                                                               >  SRR4302540.5957743/1‑94 (MQ=60)
                                 AGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATT                                                               <  SRR4302540.369652/94‑1 (MQ=60)
                                 AGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATT                                                               <  SRR4302540.5161232/94‑1 (MQ=60)
                                    GGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCC                                                            >  SRR4302540.2617465/1‑94 (MQ=60)
                                      CAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTG                                                          >  SRR4302540.1532001/1‑94 (MQ=60)
                                        GAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCA                                                        <  SRR4302540.5579813/94‑1 (MQ=60)
                                        GAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCA                                                        >  SRR4302540.7482112/1‑94 (MQ=60)
                                           AGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAAT                                                     >  SRR4302540.9306307/1‑94 (MQ=60)
                                            GTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATT                                                    >  SRR4302540.4908704/1‑94 (MQ=60)
                                             gttcGCGCAAACCTGCTTCATATTGCTGATGATCGCCCTCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTC                                                   <  SRR4302540.6809408/90‑1 (MQ=60)
                                              TTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCA                                                  >  SRR4302540.2294934/1‑94 (MQ=60)
                                                GGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATC                                                <  SRR4302540.10140592/94‑1 (MQ=60)
                                                GGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATC                                                <  SRR4302540.4862738/94‑1 (MQ=60)
                                                 GCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCA                                               <  SRR4302540.4778632/94‑1 (MQ=60)
                                                  CGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCAT                                              <  SRR4302540.9666153/94‑1 (MQ=60)
                                                  CGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCAT                                              <  SRR4302540.1031669/94‑1 (MQ=60)
                                                  CGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCAT                                              >  SRR4302540.5413509/1‑94 (MQ=60)
                                                   GCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATC                                             <  SRR4302540.6235387/94‑1 (MQ=60)
                                                   GCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGAGACGGATATTGCCTGCAAATTCATCATC                                             >  SRR4302540.2497127/1‑94 (MQ=60)
                                                     AAACCTGCTCCATATTGCTGATTGTCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCT                                           >  SRR4302540.1852918/1‑94 (MQ=60)
                                                     AAACCTGCTCCATATTGCTGATGATCGCCTCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCT                                           <  SRR4302540.9897993/94‑1 (MQ=60)
                                                     AAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCT                                           <  SRR4302540.9897992/94‑1 (MQ=60)
                                                      AACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTG                                          >  SRR4302540.8531437/1‑94 (MQ=60)
                                                        CCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAA                                        <  SRR4302540.7482842/94‑1 (MQ=60)
                                                        CCTGCTCCATATTGCTCATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAA                                        <  SRR4302540.2879087/94‑1 (MQ=60)
                                                         CTGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAAT                                       >  SRR4302540.8304041/1‑94 (MQ=60)
                                                          TGCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATC                                      >  SRR4302540.5190925/1‑94 (MQ=60)
                                                           GCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCA                                     >  SRR4302540.3234751/1‑94 (MQ=60)
                                                           GCTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCA                                     <  SRR4302540.485431/94‑1 (MQ=60)
                                                            CTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAG                                    <  SRR4302540.9815237/94‑1 (MQ=60)
                                                            CTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAG                                    <  SRR4302540.6694704/94‑1 (MQ=60)
                                                            CTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAG                                    <  SRR4302540.4656654/94‑1 (MQ=60)
                                                            CTCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAG                                    <  SRR4302540.9651726/94‑1 (MQ=60)
                                                            CTCCATATTGCTGATGATCGCCGCCCCCAG‑CGCCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCCTCATCCTGAATCAG                                    >  SRR4302540.4823134/1‑94 (MQ=60)
                                                             TCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGC                                   >  SRR4302540.1013023/1‑94 (MQ=60)
                                                             TCCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGC                                   >  SRR4302540.3180452/1‑94 (MQ=60)
                                                              CCATATTGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCG                                  <  SRR4302540.9458011/94‑1 (MQ=60)
                                                                    TGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAAT                            <  SRR4302540.1350445/94‑1 (MQ=60)
                                                                    TGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAAT                            <  SRR4302540.3055318/94‑1 (MQ=60)
                                                                    TGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAAT                            <  SRR4302540.7471307/94‑1 (MQ=60)
                                                                    TGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAAT                            <  SRR4302540.5482566/94‑1 (MQ=60)
                                                                    TGCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAAT                            <  SRR4302540.5482565/94‑1 (MQ=60)
                                                                     GCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATG                           <  SRR4302540.3040063/94‑1 (MQ=60)
                                                                     GCTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATG                           <  SRR4302540.7669330/94‑1 (MQ=60)
                                                                      CTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGG                          <  SRR4302540.3394790/94‑1 (MQ=60)
                                                                      CTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGG                          <  SRR4302540.9358356/94‑1 (MQ=60)
                                                                      CTGATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGG                          <  SRR4302540.7744403/94‑1 (MQ=60)
                                                                        GATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGC                        >  SRR4302540.7118947/1‑94 (MQ=60)
                                                                         ATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCG                       >  SRR4302540.8499811/1‑94 (MQ=60)
                                                                         ATGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCG                       >  SRR4302540.890646/1‑94 (MQ=60)
                                                                          TGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGC                      >  SRR4302540.2023145/1‑94 (MQ=60)
                                                                          TGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGC                      >  SRR4302540.3007632/1‑94 (MQ=60)
                                                                          TGATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGC                      <  SRR4302540.7282957/94‑1 (MQ=60)
                                                                           GATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCT                     <  SRR4302540.722345/94‑1 (MQ=60)
                                                                            ATCGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTG                    >  SRR4302540.4498721/1‑94 (MQ=60)
                                                                              CGCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGGGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAG                  >  SRR4302540.2846151/1‑94 (MQ=60)
                                                                               GCCGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGG                 <  SRR4302540.8474288/94‑1 (MQ=60)
                                                                                 CGCCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGAT               >  SRR4302540.2047398/1‑94 (MQ=60)
                                                                                   cccctagcC‑CCCTGATGCTGTTCTGTCATCAGGCGACGAATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAG             <  SRR4302540.247307/86‑1 (MQ=60)
                                                                                   CCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAG             <  SRR4302540.1640598/94‑1 (MQ=60)
                                                                                   CCCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAG             <  SRR4302540.1761084/94‑1 (MQ=60)
                                                                                    CCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGT            <  SRR4302540.8389450/94‑1 (MQ=60)
                                                                                    CCCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGT            >  SRR4302540.4897970/1‑94 (MQ=60)
                                                                                     CCCAGCC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTT           >  SRR4302540.7597243/1‑94 (MQ=60)
                                                                                        agcC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTG        <  SRR4302540.5985666/91‑1 (MQ=60)
                                                                                         gcC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGC       >  SRR4302540.3750013/3‑94 (MQ=60)
                                                                                         gcC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGC       <  SRR4302540.475751/92‑1 (MQ=60)
                                                                                         gcC‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGC       >  SRR4302540.4165912/3‑94 (MQ=60)
                                                                                           C‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTT     >  SRR4302540.923948/1‑94 (MQ=60)
                                                                                           C‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTT     <  SRR4302540.2050308/94‑1 (MQ=60)
                                                                                             CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCACATTCATAATCCTGAATCATCGACAAATGGGCGCTGAGGTTAGTTTTGgttc    >  SRR4302540.512398/1‑90 (MQ=60)
                                                                                             CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTTAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTT    <  SRR4302540.5750740/94‑1 (MQ=60)
                                                                                             CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTT    >  SRR4302540.8246264/1‑94 (MQ=60)
                                                                                             CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTT    >  SRR4302540.512397/1‑94 (MQ=60)
                                                                                              CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTC   <  SRR4302540.9121835/94‑1 (MQ=60)
                                                                                              CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTC   >  SRR4302540.1409668/1‑94 (MQ=60)
                                                                                              CCTGATGCTGTGCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTC   <  SRR4302540.8792388/94‑1 (MQ=60)
                                                                                               CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCG  >  SRR4302540.4012707/1‑94 (MQ=60)
                                                                                               CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCG  >  SRR4302540.4796145/1‑94 (MQ=60)
                                                                                               |                                                                                             
GTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑C‑CCCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCG  >  NC_000913/2501795‑2501981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑