Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,501,888 +C coding (618/2496 nt) b2383 ← putative PTS system enzyme IIA component, enzyme I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,501,8841.C99.4% 553.8 / ‑3.9 174coding (622/2496 nt)b2383putative PTS system enzyme IIA component, enzyme I
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base C (70/103);  total (71/103)
Fisher's exact test for biased strand distribution p-value = 4.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

Only 100 of 196 total aligned reads displayed.

AATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑CCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGT  >  NC_000913/2501792‑2501971
                                                                                             |                                                                                        
aaTGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAg                                                                                           <  1:3220178/93‑1 (MQ=255)
 aTGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑c                                                                                         >  1:3872354/1‑93 (MQ=255)
  tGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑cc                                                                                        >  1:1394153/1‑93 (MQ=255)
  tGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑cc                                                                                        >  1:447774/1‑93 (MQ=255)
     cGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑ccc‑cc                                                                                    <  1:897767/93‑2 (MQ=255)
       cTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑ccc‑cctg                                                                                  >  2:2211307/1‑90 (MQ=255)
       cTAACACGTTCGCGCAGATAATCGCTGACAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑ccc‑cctg                                                                                  >  1:1945222/1‑90 (MQ=255)
        tAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑ccc‑cctga                                                                                 <  2:2273883/93‑5 (MQ=255)
          acacGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATg                                                                                <  1:3733250/93‑1 (MQ=255)
          acacGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATg                                                                                >  2:4456427/1‑93 (MQ=255)
          acacGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATg                                                                                >  2:2055800/1‑93 (MQ=255)
           cacGTCCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGc                                                                               <  2:462727/93‑1 (MQ=255)
            acGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCt                                                                              >  2:552246/1‑93 (MQ=255)
            acGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCt                                                                              >  1:2289756/1‑93 (MQ=255)
                tCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTc                                                                          <  1:5834491/93‑1 (MQ=255)
                   cgcAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGt                                                                       <  1:4222224/93‑1 (MQ=255)
                   cgcAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGt                                                                       <  1:1744716/93‑1 (MQ=255)
                     cAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGtca                                                                     <  2:2405586/93‑1 (MQ=255)
                     cAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGtca                                                                     <  2:1643824/93‑1 (MQ=255)
                     cAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGtca                                                                     >  2:5343368/1‑93 (MQ=255)
                      aGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGtcat                                                                    >  2:1872819/1‑93 (MQ=255)
                       gATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGtcatc                                                                   >  2:2622902/1‑93 (MQ=255)
                       gATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGtcatc                                                                   <  2:1959710/93‑1 (MQ=255)
                        aTAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGtcatca                                                                  >  1:4837389/1‑93 (MQ=255)
                         tAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAg                                                                 <  2:3758683/93‑1 (MQ=255)
                         tAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAg                                                                 >  2:824283/1‑93 (MQ=255)
                          aaTCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAgg                                                                <  1:4973428/93‑1 (MQ=255)
                            tCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCg                                                              >  2:4402326/1‑93 (MQ=255)
                            tCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCg                                                              >  1:2975219/1‑93 (MQ=255)
                              gCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGAc                                                            >  1:3534406/1‑93 (MQ=255)
                               cTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACg                                                           >  1:3662269/1‑93 (MQ=255)
                               cTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACg                                                           <  1:6136229/93‑1 (MQ=255)
                               cTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACg                                                           <  2:3967383/93‑1 (MQ=255)
                                tGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACgg                                                          <  1:3416202/93‑1 (MQ=255)
                                tGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACgg                                                          >  1:1404000/1‑93 (MQ=255)
                                  gcagaggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGat                                                        <  2:4705507/93‑1 (MQ=255)
                                  gcagaggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGat                                                        <  1:1472458/93‑1 (MQ=255)
                                   cagaggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGata                                                       >  1:972660/1‑93 (MQ=255)
                                     gaggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATAtt                                                     >  2:1143235/1‑93 (MQ=255)
                                     gaggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATAtt                                                     <  1:6081151/93‑1 (MQ=255)
                                       ggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGc                                                   <  2:1815225/93‑1 (MQ=255)
                                       ggcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGc                                                   <  1:5523647/93‑1 (MQ=255)
                                        gcagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGcc                                                  <  2:831672/93‑1 (MQ=255)
                                         cagaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCt                                                 >  2:3451661/1‑93 (MQ=255)
                                          agaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTg                                                >  2:2836130/1‑93 (MQ=255)
                                           gaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGc                                               <  2:3562673/93‑1 (MQ=255)
                                           gaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGc                                               <  1:2888544/93‑1 (MQ=255)
                                           gaAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGc                                               <  1:2941386/93‑1 (MQ=255)
                                            aaaGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCa                                              <  1:5208931/93‑1 (MQ=255)
                                                   ggCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATtcat                                       >  1:2857735/1‑93 (MQ=255)
                                                    gcgcAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATtcatc                                      >  1:5697526/1‑93 (MQ=255)
                                                       cAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CCGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCatcatc                                   <  1:3613670/93‑1 (MQ=255)
                                                        aaaCCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATcc                                  >  2:5011162/1‑93 (MQ=255)
                                                        aaaCCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATcc                                  <  2:4508783/93‑1 (MQ=255)
                                                        aaaCCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATcc                                  >  1:4720073/1‑93 (MQ=255)
                                                         aaCCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCt                                 <  1:3077064/93‑1 (MQ=255)
                                                         aaCCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCt                                 >  2:4246262/1‑93 (MQ=255)
                                                         aaCCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCt                                 <  1:4012791/93‑1 (MQ=255)
                                                          aCCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGACTGCAAATTCATCATCCTg                                >  2:5753280/1‑93 (MQ=255)
                                                            cTGCTCCATATTGCTGATGATGGCCGCCCCCAG‑CCCACTGATGCTGTTCTGTCATCAGGCGAAGGATATTGCCTGCACATTCATCATGCTGaa                              >  2:2432216/1‑93 (MQ=255)
                                                            cTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGaa                              <  1:3174382/93‑1 (MQ=255)
                                                            cTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGaa                              <  1:2415636/93‑1 (MQ=255)
                                                                tCCATATTGCTGATGATCGCCGCCCCCCGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATGTTGCCTGCAAATTCATCATCCTGAATCAg                          >  2:4186509/1‑93 (MQ=255)
                                                                   atatTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGa                       >  1:3314442/1‑93 (MQ=255)
                                                                   atatTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGa                       >  2:3847163/1‑93 (MQ=255)
                                                                   atatTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGa                       <  2:605668/93‑1 (MQ=255)
                                                                   atatTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGa                       >  1:5313739/1‑93 (MQ=255)
                                                                   atacTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGa                       <  2:2991477/93‑1 (MQ=255)
                                                                    tatTGCTGATGTTCGCCTCCTCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCGGCAAATTCATCATCCTGAATCAGCGAc                      <  2:3610301/93‑1 (MQ=255)
                                                                    tatTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGAc                      >  2:5987423/1‑93 (MQ=255)
                                                                    tatTGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGAc                      >  2:1518346/1‑93 (MQ=255)
                                                                       tGCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACaaa                   <  1:940817/93‑1 (MQ=255)
                                                                        gCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAAt                  <  1:3624869/93‑1 (MQ=255)
                                                                        gCTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAAt                  <  2:5144893/93‑1 (MQ=255)
                                                                         cTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATg                 <  2:5652942/93‑1 (MQ=255)
                                                                         cTGATGATCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATg                 <  2:2184483/93‑1 (MQ=255)
                                                                          tgatgaTCGCCTCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATgg                <  1:5177871/93‑1 (MQ=255)
                                                                          tgatgaTCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATgg                <  1:4849660/93‑1 (MQ=255)
                                                                          tgatgaTCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATgg                <  2:4788772/93‑1 (MQ=255)
                                                                           gatgatCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATtgg               >  1:4608810/1‑93 (MQ=255)
                                                                           gatgatCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATggg               >  2:1839315/1‑93 (MQ=255)
                                                                           gatgatCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATggg               <  1:4160055/93‑1 (MQ=255)
                                                                           gatgatCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATggg               <  2:3261185/93‑1 (MQ=255)
                                                                           gatgatCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATggg               <  1:469043/93‑1 (MQ=255)
                                                                           gatgatCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATggg               >  1:755776/1‑93 (MQ=255)
                                                                           gatgatCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATggg               >  1:5975839/1‑93 (MQ=255)
                                                                            atgatCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGgc              <  1:655396/93‑1 (MQ=255)
                                                                              gatCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGgcgc            <  2:2918543/93‑1 (MQ=255)
                                                                               atCGCCGCCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCt           <  2:5214129/93‑1 (MQ=255)
                                                                                 cgccgcCCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGa         <  2:5933003/93‑1 (MQ=255)
                                                                                  gccgccCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAg        <  2:2727094/93‑1 (MQ=255)
                                                                                  gccgccCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAg        <  1:1641464/93‑1 (MQ=255)
                                                                                    cgccCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGa      <  2:4929876/93‑1 (MQ=255)
                                                                                     gccCCCAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGAt     <  2:4894872/93‑1 (MQ=255)
                                                                                       ccccAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAg   >  1:5422696/1‑93 (MQ=255)
                                                                                       ccccAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAg   >  1:4542290/1‑93 (MQ=255)
                                                                                       ccccAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAg   >  1:5098733/1‑93 (MQ=255)
                                                                                        cccAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGt  >  1:151901/1‑93 (MQ=255)
                                                                                        cccAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGt  >  1:6259278/1‑93 (MQ=255)
                                                                                        cccAGCCCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCACCCGGAATCAGCGACAAATGGGCGCTGAGGATAGt  <  2:3321035/93‑1 (MQ=255)
                                                                                             |                                                                                        
AATGTCGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑CCC‑CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGT  >  NC_000913/2501792‑2501971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑CC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCG  >  NC_000913/2501797‑2501981
                                                                                             |                                                                                             
CGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑CC‑CCct                                                                                             <  SRR4302533.903037/94‑3 (MQ=60)
CGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑CC‑CCct                                                                                             <  SRR4302533.6160433/94‑3 (MQ=60)
 GCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑CCTCCTG                                                                                             >  SRR4302533.2239464/1‑94 (MQ=60)
 GCTAACACGTTCGCGCAGATAATCGCTGACAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑CC‑CCctg                                                                                            >  SRR4302533.1957085/1‑91 (MQ=60)
  CTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGA                                                                                            >  SRR4302533.5547950/1‑94 (MQ=60)
   TAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGAT                                                                                           >  SRR4302533.5335456/1‑94 (MQ=60)
     ACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGC                                                                                         <  SRR4302533.3756152/94‑1 (MQ=60)
      CACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCT                                                                                        >  SRR4302533.2303934/1‑94 (MQ=60)
        CGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGT                                                                                      >  SRR4302533.3153762/1‑94 (MQ=60)
         GTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTT                                                                                     >  SRR4302533.5087038/1‑94 (MQ=60)
           TCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCT                                                                                   <  SRR4302533.5869750/94‑1 (MQ=60)
              CGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTC                                                                                <  SRR4302533.4247753/94‑1 (MQ=60)
              CGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTC                                                                                <  SRR4302533.4523821/94‑1 (MQ=60)
              CGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTC                                                                                <  SRR4302533.1755325/94‑1 (MQ=60)
                CAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCAT                                                                              >  SRR4302533.2908076/1‑94 (MQ=60)
                CAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCAT                                                                              >  SRR4302533.2973829/1‑94 (MQ=60)
                CAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCAT                                                                              <  SRR4302533.4491940/94‑1 (MQ=60)
                 AGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATC                                                                             <  SRR4302533.3589618/94‑1 (MQ=60)
                  GATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCA                                                                            >  SRR4302533.4866706/1‑94 (MQ=60)
                   ATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAG                                                                           <  SRR4302533.5884558/94‑1 (MQ=60)
                   ATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAG                                                                           <  SRR4302533.4164006/94‑1 (MQ=60)
                    TAATCGCTGGCAGAGGCAGAAAGTTTGTCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGG                                                                          <  SRR4302533.2378045/94‑1 (MQ=60)
                    TAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGG                                                                          >  SRR4302533.6323514/1‑94 (MQ=60)
                     AATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGC                                                                         <  SRR4302533.5003584/94‑1 (MQ=60)
                      ATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCG                                                                        >  SRR4302533.2993423/1‑94 (MQ=60)
                        CGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGAC                                                                      >  SRR4302533.3556187/1‑94 (MQ=60)
                         GCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACG                                                                     <  SRR4302533.3787125/94‑1 (MQ=60)
                         GCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACG                                                                     >  SRR4302533.3684783/1‑94 (MQ=60)
                         GCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACG                                                                     <  SRR4302533.516763/94‑1 (MQ=60)
                          CTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGG                                                                    >  SRR4302533.1412498/1‑94 (MQ=60)
                          CTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGG                                                                    <  SRR4302533.6173198/94‑1 (MQ=60)
                           TGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGA                                                                   <  SRR4302533.3437308/94‑1 (MQ=60)
                             GCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATA                                                                 >  SRR4302533.978371/1‑94 (MQ=60)
                             GCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATA                                                                 <  SRR4302533.1481357/94‑1 (MQ=60)
                             GCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATA                                                                 <  SRR4302533.6113999/94‑1 (MQ=60)
                              CAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATAT                                                                >  SRR4302533.186182/1‑94 (MQ=60)
                              CAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATAT                                                                <  SRR4302533.351586/94‑1 (MQ=60)
                               AGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATT                                                               >  SRR4302533.661678/1‑94 (MQ=60)
                                GAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTG                                                              <  SRR4302533.6117925/94‑1 (MQ=60)
                                  GGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCC                                                            <  SRR4302533.5556997/94‑1 (MQ=60)
                                   GCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCT                                                           <  SRR4302533.5266162/94‑1 (MQ=60)
                                    CAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTG                                                          <  SRR4302533.6270480/94‑1 (MQ=60)
                                      GAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCA                                                        <  SRR4302533.2906300/94‑1 (MQ=60)
                                      GAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCA                                                        <  SRR4302533.2959349/94‑1 (MQ=60)
                                       AAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAA                                                       <  SRR4302533.5240308/94‑1 (MQ=60)
                                          GTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATT                                                    <  SRR4302533.995291/94‑1 (MQ=60)
                                           TTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTC                                                   <  SRR4302533.4878170/94‑1 (MQ=60)
                                             TGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCAT                                                 >  SRR4302533.2875317/1‑94 (MQ=60)
                                              GGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATC                                                <  SRR4302533.789855/94‑1 (MQ=60)
                                              GGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATC                                                >  SRR4302533.5731943/1‑94 (MQ=60)
                                               GCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCA                                               >  SRR4302533.5396832/1‑94 (MQ=60)
                                                CGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCAT                                              >  SRR4302533.1733490/1‑94 (MQ=60)
                                                  CAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCC                                            >  SRR4302533.4748693/1‑94 (MQ=60)
                                                  CAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCCGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCC                                            <  SRR4302533.3635906/94‑1 (MQ=60)
                                                    AACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTG                                          <  SRR4302533.4037224/94‑1 (MQ=60)
                                                    AACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTG                                          <  SRR4302533.3095948/94‑1 (MQ=60)
                                                    AACCTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTG                                          <  SRR4302533.3217564/94‑1 (MQ=60)
                                                       CTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAAT                                       <  SRR4302533.2430559/94‑1 (MQ=60)
                                                       CTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAAT                                       <  SRR4302533.3057021/94‑1 (MQ=60)
                                                       CTGCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAAT                                       <  SRR4302533.3193916/94‑1 (MQ=60)
                                                         GCTCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCA                                     >  SRR4302533.1485310/1‑94 (MQ=60)
                                                           TCCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGC                                   >  SRR4302533.4742671/1‑94 (MQ=60)
                                                            CCATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCG                                  <  SRR4302533.4696526/94‑1 (MQ=60)
                                                             CATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGA                                 >  SRR4302533.5345580/1‑94 (MQ=60)
                                                             CATATTGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGA                                 >  SRR4302533.3334905/1‑94 (MQ=60)
                                                                  TGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAAT                            <  SRR4302533.946343/94‑1 (MQ=60)
                                                                  TGCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAAT                            <  SRR4302533.5795932/94‑1 (MQ=60)
                                                                   GCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATG                           <  SRR4302533.3647173/94‑1 (MQ=60)
                                                                   GCTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATG                           <  SRR4302533.3647172/94‑1 (MQ=60)
                                                                    CTGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGG                          <  SRR4302533.3930003/94‑1 (MQ=60)
                                                                     TGATGATCGCCTCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGG                         <  SRR4302533.5209142/94‑1 (MQ=60)
                                                                     TGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATTGG                         >  SRR4302533.4636722/1‑94 (MQ=60)
                                                                     TGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGG                         >  SRR4302533.6012014/1‑94 (MQ=60)
                                                                     TGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGG                         >  SRR4302533.760223/1‑94 (MQ=60)
                                                                     TGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGG                         <  SRR4302533.4879049/94‑1 (MQ=60)
                                                                     TGATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGG                         >  SRR4302533.3262294/1‑94 (MQ=60)
                                                                      GATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGC                        <  SRR4302533.4185268/94‑1 (MQ=60)
                                                                      GATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGC                        >  SRR4302533.3703992/1‑94 (MQ=60)
                                                                      GATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGC                        <  SRR4302533.471845/94‑1 (MQ=60)
                                                                      GATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGC                        <  SRR4302533.1972916/94‑1 (MQ=60)
                                                                      GATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGC                        <  SRR4302533.4595838/94‑1 (MQ=60)
                                                                       ATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCG                       >  SRR4302533.1480043/1‑94 (MQ=60)
                                                                       ATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCG                       <  SRR4302533.659264/94‑1 (MQ=60)
                                                                       ATGATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCG                       >  SRR4302533.5694505/1‑94 (MQ=60)
                                                                         GATCGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCT                     <  SRR4302533.911168/94‑1 (MQ=60)
                                                                            CGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAG                  <  SRR4302533.3407152/94‑1 (MQ=60)
                                                                            CGCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAG                  >  SRR4302533.247593/1‑94 (MQ=60)
                                                                             GCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGG                 <  SRR4302533.1651408/94‑1 (MQ=60)
                                                                             GCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGG                 <  SRR4302533.1598327/94‑1 (MQ=60)
                                                                             GCCGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGG                 <  SRR4302533.5233798/94‑1 (MQ=60)
                                                                               CGCCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGAT               >  SRR4302533.2888810/1‑94 (MQ=60)
                                                                                 CCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAG             >  SRR4302533.4569797/1‑94 (MQ=60)
                                                                                 CCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAG             >  SRR4302533.5129537/1‑94 (MQ=60)
                                                                                 CCCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAG             >  SRR4302533.5455327/1‑94 (MQ=60)
                                                                                  CCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGT            >  SRR4302533.152872/1‑94 (MQ=60)
                                                                                  CCCCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGT            >  SRR4302533.6296704/1‑94 (MQ=60)
                                                                                    CCAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTT          <  SRR4302533.1439075/94‑1 (MQ=60)
                                                                                     CAGCCC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTT         <  SRR4302533.3677549/94‑1 (MQ=60)
                                                                                          C‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTT    <  SRR4302533.2224943/94‑1 (MQ=60)
                                                                                          C‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTT    <  SRR4302533.2703704/94‑1 (MQ=60)
                                                                                             CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCG  <  SRR4302533.288637/94‑1 (MQ=60)
                                                                                             CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCG  <  SRR4302533.3503356/94‑1 (MQ=60)
                                                                                             CTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCG  >  SRR4302533.322386/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGCTAACACGTTCGCGCAGATAATCGCTGGCAGAGGCAGAAAGTTTGGCGCAAACCTGCTCCATATTGCTGATGATCGCCGCCCCCAG‑CC‑CCTGATGCTGTTCTGTCATCAGGCGACGGATATTGCCTGCAAATTCATCATCCTGAATCAGCGACAAATGGGCGCTGAGGATAGTTTTGCTTTCG  >  NC_000913/2501797‑2501981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑