Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,505,495	C→T	Q140Q (CAG→CAA)	b2386 ←	putative transport protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,505,495	0	C	T	100.0%	619.7 / NA	180	Q140Q (CAG→CAA)	b2386	putative transport protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (152/28); total (152/28)

Only 100 of 216 total aligned reads displayed.

AATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAA  >  NC_000913/2505405‑2505583

 ataatCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTgg                                                                                       <  2:468027/93‑1 (MQ=255)

 ataatCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTgg                                                                                       >  2:5316056/1‑93 (MQ=255)

  taatCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTggg                                                                                      >  2:9461068/1‑93 (MQ=255)

   aatCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGt                                                                                     <  2:403122/93‑1 (MQ=255)

    atCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTGAGCAGTTGCGTCGGTTgggca                                                                                    >  2:1520990/1‑91 (MQ=255)

    atCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTa                                                                                    >  1:845148/1‑93 (MQ=255)

     tCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAg                                                                                   >  2:2972309/1‑93 (MQ=255)

     tCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAg                                                                                   <  1:9761703/93‑1 (MQ=255)

      cAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGa                                                                                  <  2:492860/93‑1 (MQ=255)

      cAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGa                                                                                  <  1:9299090/93‑1 (MQ=255)

      cAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCACCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGa                                                                                  <  1:8146842/93‑1 (MQ=255)

       aGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGAc                                                                                 <  1:1534701/93‑1 (MQ=255)

       aGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGAc                                                                                 >  2:1174541/1‑93 (MQ=255)

        gcgcACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACa                                                                                >  2:7747070/1‑93 (MQ=255)

        gcgcACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACa                                                                                >  1:7299248/1‑93 (MQ=255)

        gcgcACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACa                                                                                >  1:8981331/1‑93 (MQ=255)

           cACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCa                                                                             >  2:4804985/1‑93 (MQ=255)

            aCCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCaa                                                                            <  1:6016459/93‑1 (MQ=255)

             ccAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAAc                                                                           >  2:6850494/1‑93 (MQ=255)

             ccAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAAc                                                                           <  1:6048819/93‑1 (MQ=255)

              cAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAAcc                                                                          >  1:7383926/1‑93 (MQ=255)

              cAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATAAAcc                                                                          >  2:2722332/1‑93 (MQ=255)

                aTGAAGGTGGACGGCACCGGCGAAGAGGGCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGGAGACATCAACccg                                                                        >  1:4891441/1‑93 (MQ=255)

                 tGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCCACccgc                                                                       >  2:2743058/1‑93 (MQ=255)

                 tGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACccgc                                                                       <  2:754988/93‑1 (MQ=255)

                 tGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACccgc                                                                       >  2:2122946/1‑93 (MQ=255)

                  gAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACccgcc                                                                      >  1:1421995/1‑93 (MQ=255)

                  gAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACccgcc                                                                      <  1:3158562/93‑1 (MQ=255)

                   aaGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACccgccg                                                                     >  2:9122190/1‑93 (MQ=255)

                    aGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGa                                                                    >  1:439749/1‑93 (MQ=255)

                        ggACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATaaa                                                                >  1:3864233/1‑93 (MQ=255)

                        ggACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATaaa                                                                >  1:4514912/1‑93 (MQ=255)

                            ggCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCa                                                            >  2:26289/1‑93 (MQ=255)

                             gCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAg                                                           >  1:3799565/1‑93 (MQ=255)

                              cACGGGCGAAGAGGTCGCCCTTTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTGGGTTGGGTAGACATCACCCCGCCGATAAAACCAGc                                                          <  2:1467655/93‑1 (MQ=255)

                                ccGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCg                                                        >  1:5941915/1‑93 (MQ=255)

                                 cGGGGAGGAGGTCGCCCATTGCATAGTGCGGGGATCGAAGTTCAGCGGTTGCGTGGGTTGGGTAGACATCACCCCGCCGATAAAACCAGCCgg                                                       <  2:4593768/93‑1 (MQ=255)

                                  ggCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCggg                                                      >  1:3720993/1‑93 (MQ=255)

                                   gaaaaGAGGTCCCCCATTAAATGGTGCGGGGATCAAAGTTCAGCATTGGGTTGGGTTGGGAAGAAATCACCCCGCCGATAAAACCAGCCGGGa                                                     <  2:3313639/90‑1 (MQ=255)

                                   gCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGa                                                     >  1:5794705/1‑93 (MQ=255)

                                   gCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGa                                                     >  1:3448012/1‑93 (MQ=255)

                                    cGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGaa                                                    >  2:3404310/1‑93 (MQ=255)

                                      aaGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACg                                                  >  2:2369769/1‑93 (MQ=255)

                                       agagGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGc                                                 >  1:7769326/1‑93 (MQ=255)

                                          ggTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAg                                              >  1:2071627/1‑93 (MQ=255)

                                            tCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGtt                                            >  1:330948/1‑93 (MQ=255)

                                              gCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTg                                          >  1:1662759/1‑93 (MQ=255)

                                               cccATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCTGCCGATAAAACCAGCCGGGAACGACAGTTTGc                                         >  1:1755139/1‑93 (MQ=255)

                                                ccATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGcc                                        >  2:1981242/1‑93 (MQ=255)

                                                ccATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGcc                                        >  1:5154311/1‑93 (MQ=255)

                                                 cATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAAGCAGCCGGGAACGCCAGTTTGCCa                                       >  1:7909838/1‑93 (MQ=255)

                                                 cATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCa                                       >  1:6340562/1‑93 (MQ=255)

                                                 cATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCa                                       >  1:6169274/1‑93 (MQ=255)

                                                  aTTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCAc                                      >  2:3182400/1‑93 (MQ=255)

                                                  aTTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCAGGGAACGCCAGTTTGCCAc                                      >  1:2555875/1‑93 (MQ=255)

                                                  aTTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGAGTCGGTTGGGGAGACATCAACCCGCCGATAAAACTAGCCGGGGACGCCAGTGTGCCAc                                      >  1:3956821/1‑93 (MQ=255)

                                                   ttGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCAcc                                     >  1:5455504/1‑93 (MQ=255)

                                                      cATTGTGGTGGAATGGAAGTTCAGCAGTGGCGTTGTTGGGGTAAACACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGAt                                  <  1:2274242/93‑1 (MQ=255)

                                                         aGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATaga                               >  1:2950699/1‑93 (MQ=255)

                                                         aGTGCGGGAATCAAGTTTCAGCAGTTGCGTGGGTTGGGTAGACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATaga                               <  2:1671325/93‑1 (MQ=255)

                                                          gTGCTGGAATCAAAGTTCAGCGGTTGCGTCGTTGGGGTAGACACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATagag                              <  1:4480841/93‑1 (MQ=255)

                                                           tGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGt                             >  1:6960/1‑93 (MQ=255)

                                                            gcggggATCGAAGTTCAGCGGTTGGGTGGTTGGGGAAGACACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGtt                            <  2:9716454/90‑1 (MQ=255)

                                                            gCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCAGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGtt                            >  1:8245454/1‑93 (MQ=255)

                                                             gggggAGCGAGGTTCAGCTGTGGGGGTGTTTGGGTAGACAACACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTc                           <  1:6444883/91‑1 (MQ=255)

                                                             cTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTc                           >  2:4383939/1‑93 (MQ=255)

                                                             cTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTc                           >  2:5835918/1‑93 (MQ=255)

                                                               gggATCAAGTTTAAGCGGTTGCGTGGGTGGGGAAGACACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGc                         <  2:5985170/93‑1 (MQ=255)

                                                                ggATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGcc                        >  2:8481038/1‑93 (MQ=255)

                                                                ggATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGcc                        >  1:3322230/1‑93 (MQ=255)

                                                                 gATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCca                       >  2:2255372/1‑93 (MQ=255)

                                                                  aTCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCcac                      >  1:805363/1‑93 (MQ=255)

                                                                  aTCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCcac                      >  2:9679475/1‑93 (MQ=255)

                                                                   tCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCTATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCcaca                     >  2:7306421/1‑93 (MQ=255)

                                                                     gAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGTCACaaa                   >  2:7506222/1‑93 (MQ=255)

                                                                     gAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACaaa                   >  2:1256143/1‑93 (MQ=255)

                                                                      aaGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACaaaa                  >  2:7721474/1‑93 (MQ=255)

                                                                      aaGTTCACCAGTTGCGTGGGTGGGGTAGCCACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACaaaa                  <  2:4845807/93‑1 (MQ=255)

                                                                        gTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAagc                >  1:8537114/1‑93 (MQ=255)

                                                                         ttCAGCGGTGGGGTCGGTTGGGTAGACACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAagca               <  2:202494/93‑1 (MQ=255)

                                                                           cagcagTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAagcagc             >  2:6154244/1‑93 (MQ=255)

                                                                           cagcagTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAagcagc             >  2:6180216/1‑93 (MQ=255)

                                                                           cagcagTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTAGCCACAAAagcagc             >  1:1065634/1‑93 (MQ=255)

                                                                            agcggTTGCGTGGGTTGGGTAAACACGCCCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAgcagca            <  1:6660177/93‑1 (MQ=255)

                                                                             gcagTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCaa           >  1:5836128/1‑93 (MQ=255)

                                                                               agTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAAc         >  2:3937442/1‑93 (MQ=255)

                                                                               agTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAAc         >  2:9062622/1‑93 (MQ=255)

                                                                                gTTGCGTGGGTTGGGAAGCAACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACa        <  1:9805269/93‑1 (MQ=255)

                                                                                gTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACa        >  1:9161061/1‑93 (MQ=255)

                                                                                 ttGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAt       >  1:9540933/1‑93 (MQ=255)

                                                                                 ttGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAt       >  2:5223664/1‑93 (MQ=255)

                                                                                 ttGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAt       >  2:5023676/1‑93 (MQ=255)

                                                                                 ttGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAt       >  1:7221897/1‑93 (MQ=255)

                                                                                 ttGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAt       >  1:4780567/1‑93 (MQ=255)

                                                                                   gCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCg     >  2:1062487/1‑93 (MQ=255)

                                                                                   gCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCg     >  1:1883132/1‑93 (MQ=255)

                                                                                      tCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGaa  >  2:4142031/1‑93 (MQ=255)

                                                                                      tCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGaa  >  1:8916310/1‑93 (MQ=255)

                                                                                      tCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGaa  >  1:6561103/1‑93 (MQ=255)

                                                                                      tCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGATTGCCACCGATAGAGATCGCCACAAAAGCAGCAAACATCGGaa  >  2:9776032/1‑93 (MQ=255)

AATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAA  >  NC_000913/2505405‑2505583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGG  >  NC_000913/2505402‑2505586

AGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGT                                                                                             <  SRR4302540.4732816/94‑1 (MQ=60)

AGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGT                                                                                             >  SRR4302540.9112914/1‑94 (MQ=60)

 GAAATAATCAGCGCACCAATGAAGGTGGACGGCCCCGGCGAAGAGGTCGCACATTGAATAGTGCTGGGATCGAAGTTCAGCAGTTGCATCGGTT                                                                                            <  SRR4302540.9059221/94‑1 (MQ=60)

     TAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGT                                                                                        <  SRR4302540.9591122/94‑1 (MQ=60)

      AATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTA                                                                                       >  SRR4302540.854115/1‑94 (MQ=60)

       ATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAG                                                                                      <  SRR4302540.4642328/94‑1 (MQ=60)

       ATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAG                                                                                      >  SRR4302540.9385563/1‑94 (MQ=60)

        TCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGA                                                                                     <  SRR4302540.9876200/94‑1 (MQ=60)

         CAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGAC                                                                                    <  SRR4302540.9410178/94‑1 (MQ=60)

         CAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGAC                                                                                    <  SRR4302540.1668327/94‑1 (MQ=60)

         CAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGAC                                                                                    <  SRR4302540.5656526/94‑1 (MQ=60)

         CAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGAC                                                                                    <  SRR4302540.5656527/94‑1 (MQ=60)

         CAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCACCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGAC                                                                                    <  SRR4302540.8241416/94‑1 (MQ=60)

          AGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACA                                                                                   <  SRR4302540.1551680/94‑1 (MQ=60)

          AGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACA                                                                                   >  SRR4302540.9088404/1‑94 (MQ=60)

          AGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACA                                                                                   >  SRR4302540.7380720/1‑94 (MQ=60)

              CACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAA                                                                               <  SRR4302540.7457356/94‑1 (MQ=60)

               ACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAAC                                                                              >  SRR4302540.4976590/1‑94 (MQ=60)

               ACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAAC                                                                              >  SRR4302540.4327656/1‑94 (MQ=60)

               ACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAAC                                                                              <  SRR4302540.5905991/94‑1 (MQ=60)

               ACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAAC                                                                              <  SRR4302540.6083185/94‑1 (MQ=60)

                CCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACC                                                                             <  SRR4302540.6116029/94‑1 (MQ=60)

                CCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACC                                                                             <  SRR4302540.6116028/94‑1 (MQ=60)

                CCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACC                                                                             >  SRR4302540.7466199/1‑94 (MQ=60)

                  AATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCG                                                                           >  SRR4302540.4512288/1‑94 (MQ=60)

                  AATGAAGGTGGACGGCACCGGCGAAGAGGGCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGGAGACATCAACCCG                                                                           >  SRR4302540.4944038/1‑94 (MQ=60)

                   ATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGC                                                                          >  SRR4302540.6628672/1‑94 (MQ=60)

                   ATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGC                                                                          >  SRR4302540.3789265/1‑94 (MQ=60)

                    TGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCC                                                                         >  SRR4302540.7945900/1‑94 (MQ=60)

                    TGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCC                                                                         >  SRR4302540.1437375/1‑94 (MQ=60)

                     GAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCG                                                                        <  SRR4302540.3192932/94‑1 (MQ=60)

                      AAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGA                                                                       >  SRR4302540.444427/1‑94 (MQ=60)

                      AAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGA                                                                       <  SRR4302540.988643/94‑1 (MQ=60)

                      AAGGTGGACGCCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGA                                                                       <  SRR4302540.8417578/94‑1 (MQ=60)

                       AGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGAT                                                                      >  SRR4302540.8072858/1‑94 (MQ=60)

                         GTGGACGGCACGGGCGAAGAGGTCGCCCATTGCATAGTGCGGGGATCGAAGTTCAGCAGTTGCGTGGGTTGGGTAGACATCACCCCGCCGATAA                                                                    <  SRR4302540.712689/94‑1 (MQ=60)

                         GTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAA                                                                    >  SRR4302540.1687898/1‑94 (MQ=60)

                         GTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAA                                                                    >  SRR4302540.6694256/1‑94 (MQ=60)

                          gaggCGGCACCGGCGAAGAGGGCCCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTGGGTTGGGTAGACATCACCCCGCCGATAAA                                                                   <  SRR4302540.1116286/90‑1 (MQ=60)

                          TGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAA                                                                   >  SRR4302540.3906237/1‑94 (MQ=60)

                          TGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAA                                                                   >  SRR4302540.8236448/1‑94 (MQ=60)

                          TGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAA                                                                   >  SRR4302540.4563823/1‑94 (MQ=60)

                          TGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAA                                                                   >  SRR4302540.2914984/1‑94 (MQ=60)

                            GACGGCACGGGGGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCGGTTGCGTGGGTGGGGTAGACATCAACCCGCCGATAAAAC                                                                 <  SRR4302540.611322/94‑1 (MQ=60)

                              CGGCGCCGGCGAAGAGGTCCCCCTTTGCATAGTGCGGGGATCGAAGTTCAGCAGTTGCGTGGGTTGGGTAGACATCACCCCGCCGATAAAACCA                                                               <  SRR4302540.9334496/94‑1 (MQ=60)

                              CGGCACGGGCGAAGAGGCCGCCATTGGAATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTGGTTTGGGAAGACATCACCCCGCCGATAAAACCA                                                               <  SRR4302540.3768721/94‑1 (MQ=36)

                              CGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCA                                                               >  SRR4302540.7398483/1‑94 (MQ=60)

                              CGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCA                                                               >  SRR4302540.3866944/1‑94 (MQ=60)

                               GGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAG                                                              >  SRR4302540.3840961/1‑94 (MQ=60)

                               GGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAG                                                              >  SRR4302540.6036925/1‑94 (MQ=60)

                                GCCCCGGCGAAGGGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCTTCGTTTGGGTAGACATCACCCCGCCGATAAAACCAGC                                                             <  SRR4302540.405203/94‑1 (MQ=60)

                                GCAGCGGCGAGGAGGCCGCCCATTGAATAGTGCGGGGATCGAAGTTCAGCAGTTGCGTGGGTTGGGTAGACATCACCCCGCCGATAAAACCAGC                                                             <  SRR4302540.7847514/94‑1 (MQ=60)

                                GCACCGCCGAAGAGGTCCCCCATGGCATAGTGCTGGGATCGAAGTCCAGCAGTTGCGTCGGTTGGGTAGACATCACCCCGCCGATAAAACCAGC                                                             <  SRR4302540.8429366/94‑1 (MQ=60)

                                 gcccggggaaggggtccCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTGGTTTGGGTAGACATCACCCCGCCGATAAAACCAGCC                                                            <  SRR4302540.3443576/77‑1 (MQ=60)

                                 CCCCGGCGAAGGGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGTTGGGGTAGACATCACCCCGCCGATAAAACCAGCC                                                            <  SRR4302540.4145788/94‑1 (MQ=60)

                                 CACGGGCGAAGAGGCCCCCCATTGCATAGTGCGGGGATCGAAGTTCAGCGGTTGCGGGGTTTGGGTAGACATAACCCCGCCGATAAAACCAGCC                                                            <  SRR4302540.2027157/94‑1 (MQ=34)

                                  ACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCG                                                           >  SRR4302540.6007603/1‑94 (MQ=60)

                                   CCGGCGAAGGGGTCGCCCATTGCATGGTGCTGGAATCAAAGTTCAGCAGTTGCGTCGGTGGGTTAAACATCACCCCGCCGATAAAACCAGCCGG                                                          <  SRR4302540.2021515/94‑1 (MQ=53)

                                    CGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGG                                                         >  SRR4302540.3761592/1‑94 (MQ=60)

                                     GGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGA                                                        >  SRR4302540.3485513/1‑94 (MQ=60)

                                     GGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGA                                                        >  SRR4302540.5858737/1‑94 (MQ=60)

                                     GGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGGAGACATCAACCCGCCGATAAAACCAGCCGGGA                                                        >  SRR4302540.6465819/1‑94 (MQ=60)

                                       ggaagagtcccccctTTGCATGGTGCTGGGATCGAAGTTCAGCGTTTGCGTCGTTGGGGTAGACATCACCCCGCCGATAAAACCAGCCGGGAAC                                                      <  SRR4302540.5691094/79‑1 (MQ=49)

                                       CGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGAGATCAACCCGCCGATAAAACCAGCCGGGAAC                                                      >  SRR4302540.4547727/1‑94 (MQ=60)

                                       CGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAAC                                                      >  SRR4302540.9258454/1‑94 (MQ=60)

                                        GAAGAGGTCGCCTATTGCATGGTGCGGGGATGGAGTTTCAGCAGTTGCGTCGGTGGGGTAGACATCACCCCGCCGATAAAACCAGCCGGGAACG                                                     <  SRR4302540.556505/94‑1 (MQ=53)

                                         AAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGC                                                    >  SRR4302540.7860158/1‑94 (MQ=60)

                                           GAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCG                                                  >  SRR4302540.3628281/1‑94 (MQ=60)

                                           GAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCA                                                  >  SRR4302540.5242791/1‑94 (MQ=60)

                                            AGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAG                                                 >  SRR4302540.2093906/1‑94 (MQ=60)

                                            AGGTCCCCCATTGCATGGGGGGGGGATCGAAGTTAAGCAGTTGCGTCGGTTGGGTAGACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAG                                                 <  SRR4302540.3710422/94‑1 (MQ=60)

                                             GGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGT                                                >  SRR4302540.2116437/1‑94 (MQ=60)

                                              gcccccagttggATAGTGCTGGGATCGAAGTTCAGCGGTGGCGTGGGTTGGGTAGACACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTT                                               <  SRR4302540.6434274/82‑1 (MQ=60)

                                              GTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTT                                               >  SRR4302540.334271/1‑94 (MQ=60)

                                               TCGCCCATTGCATGGTGCGGGGATCGAAGTTCAGCAGTTGCGTGGGTTGGGTAGACACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTT                                              <  SRR4302540.3412955/94‑1 (MQ=60)

                                               TCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTT                                              >  SRR4302540.6456596/1‑94 (MQ=60)

                                                CGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTG                                             >  SRR4302540.2017506/1‑94 (MQ=60)

                                                CGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTG                                             >  SRR4302540.3365360/1‑94 (MQ=60)

                                                CGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTG                                             >  SRR4302540.1680863/1‑94 (MQ=60)

                                                 GCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCTGCCGATAAAACCAGCCGGGAACGACAGTTTGC                                            >  SRR4302540.1774159/1‑94 (MQ=60)

                                                  CCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCC                                           >  SRR4302540.5210250/1‑94 (MQ=60)

                                                   CCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAAGCAGCCGGGAACGCCAGTTTGCCA                                          >  SRR4302540.8002072/1‑94 (MQ=60)

                                                   CCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCA                                          >  SRR4302540.6238012/1‑94 (MQ=60)

                                                   CCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCA                                          >  SRR4302540.6411174/1‑94 (MQ=60)

                                                   CCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCA                                          >  SRR4302540.1802822/1‑94 (MQ=60)

                                                    CATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCAC                                         >  SRR4302540.7349459/1‑94 (MQ=60)

                                                    CATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCAGGGAACGCCAGTTTGCCAC                                         >  SRR4302540.2583411/1‑94 (MQ=60)

                                                    CATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGAGTCGGTTGGGGAGACATCAACCCGCCGATAAAACTAGCCGGGGACGCCAGTGTGCCAC                                         >  SRR4302540.3999512/1‑94 (MQ=60)

                                                    CATTGCATAGTGCTGGGATCGAAGTACAGCGGTTGCGGTGGTTGGGTAAACACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCAC                                         <  SRR4302540.4468981/94‑1 (MQ=60)

                                                     tttgaatagggggGGGATGGAAGTTCAGCATTTGCGTCGTTGGGGTAGACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACC                                        <  SRR4302540.1660092/81‑1 (MQ=60)

                                                     atgcgatagggcgggtATCGAAGTTAAGCAGTTGGGTCGTTGGGGTAGACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACC                                        <  SRR4302540.6142002/78‑1 (MQ=60)

                                                     ATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACC                                        >  SRR4302540.3203312/1‑94 (MQ=60)

                                                     ATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACC                                        >  SRR4302540.5515554/1‑94 (MQ=60)

                                                       TGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGA                                      >  SRR4302540.7759998/1‑94 (MQ=60)

                                                        GCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGAT                                     >  SRR4302540.8421162/1‑94 (MQ=60)

                                                         CATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATA                                    >  SRR4302540.1397664/1‑94 (MQ=60)

                                                           TAGTGTGGGGATGGAAGTGCAGCAGTTGCGTGGGTTGGGTAGACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGA                                  <  SRR4302540.6276888/94‑1 (MQ=60)

                                                           TAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGA                                  >  SRR4302540.2983183/1‑94 (MQ=60)

                                                            AGCGCGGGGAGCGAATTTCAGCAGTTGCGTGGGTTGGGTAGACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAG                                 <  SRR4302540.6688781/94‑1 (MQ=60)

                                                             GTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGT                                >  SRR4302540.8559949/1‑94 (MQ=60)

                                                             GTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGT                                >  SRR4302540.7037/1‑94 (MQ=60)

                                                             GTGCTGGAATCAAAGTTCAGCGGTTGCGTCGTTGGGGTAGACACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGT                                <  SRR4302540.4529434/94‑1 (MQ=60)

                                                              TGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTT                               >  SRR4302540.863025/1‑94 (MQ=60)

                                                              TGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTT                               >  SRR4302540.6174172/1‑94 (MQ=60)

                                                              TGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTT                               >  SRR4302540.2983751/1‑94 (MQ=60)

                                                              TGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCAGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTT                               >  SRR4302540.8341290/1‑94 (MQ=60)

                                                                 TGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGC                            >  SRR4302540.9851240/1‑94 (MQ=60)

                                                                  GGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCGGGCAACGCCAGTTTCCCACCGATCGAGTTCGCA                           >  SRR4302540.3358674/1‑94 (MQ=60)

                                                                  GGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCC                           >  SRR4302540.3358675/1‑94 (MQ=60)

                                                                  GGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGGAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCC                           >  SRR4302540.9601304/1‑94 (MQ=60)

                                                                    GATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCAC                         >  SRR4302540.813920/1‑94 (MQ=60)

                                                                     ATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACA                        >  SRR4302540.3378238/1‑94 (MQ=60)

                                                                         AATTTCAGCAGTTGCGTCGTTTGGGTAGACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAG                    <  SRR4302540.5867296/94‑1 (MQ=60)

                                                                          AGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGC                   >  SRR4302540.8636315/1‑94 (MQ=60)

                                                                          AGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGC                   >  SRR4302540.8321976/1‑94 (MQ=60)

                                                                           GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCA                  >  SRR4302540.4894404/1‑94 (MQ=60)

                                                                            TTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAG                 >  SRR4302540.9035938/1‑94 (MQ=60)

                                                                            TTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGGTCGCCACAAAAGCAC                 >  SRR4302540.6912710/1‑94 (MQ=60)

                                                                             TCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTAGCCACAAAAGCAGC                >  SRR4302540.1076490/1‑94 (MQ=60)

                                                                               AGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAA              >  SRR4302540.5900659/1‑94 (MQ=60)

                                                                                 CAGTGGCGTGGGTTGGGTAGACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAAC            <  SRR4302540.2568830/94‑1 (MQ=60)

                                                                                  AGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACA           >  SRR4302540.9271009/1‑94 (MQ=60)

                                                                                   GTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAT          >  SRR4302540.4831659/1‑94 (MQ=60)

                                                                                   GTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAT          >  SRR4302540.9653835/1‑94 (MQ=60)

                                                                                   GTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAT          >  SRR4302540.7269795/1‑94 (MQ=60)

                                                                                   GTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACAT          >  SRR4302540.7302398/1‑94 (MQ=60)

                                                                                     TGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCG        >  SRR4302540.1903762/1‑94 (MQ=60)

                                                                                        GTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAA     >  SRR4302540.9019576/1‑94 (MQ=60)

                                                                                        GTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAA     >  SRR4302540.6633838/1‑94 (MQ=60)

                                                                                          CGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATG   >  SRR4302540.5553396/1‑94 (MQ=60)

                                                                                           GGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGG  >  SRR4302540.9507488/1‑94 (MQ=60)

AGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGG  >  NC_000913/2505402‑2505586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`