Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,505,495 C→T Q140Q (CAG→CAA)  b2386 ← putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,505,4950CT99.2% 416.5 / NA 120Q140Q (CAG→CAA) b2386putative transport protein
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (97/21);  total (99/21)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

AATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAAT  >  NC_000913/2505405‑2505584
                                                                                           |                                                                                         
aataatCAGCGCAGCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTg                                                                                         <  2:3727656/93‑1 (MQ=255)
aataatCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGCTg                                                                                         >  1:469977/1‑93 (MQ=255)
 ataatCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTgg                                                                                        >  2:4819106/1‑93 (MQ=255)
 ataatCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTgg                                                                                        <  2:1060598/93‑1 (MQ=255)
 ataatCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGCTGCGTCGGTTgg                                                                                        <  1:3403439/93‑1 (MQ=255)
 agaatCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTgg                                                                                        <  2:5346895/91‑1 (MQ=255)
  taatCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGACGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGGCGGTTggg                                                                                       >  2:3882694/1‑93 (MQ=255)
    atCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTa                                                                                     >  2:2147396/1‑93 (MQ=255)
     tCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAg                                                                                    >  1:2957661/1‑93 (MQ=255)
      cAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGa                                                                                   <  1:4729791/93‑1 (MQ=255)
        gcgcACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACa                                                                                 <  2:3789625/93‑1 (MQ=255)
        gcgcACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACa                                                                                 <  1:1967749/93‑1 (MQ=255)
        gcgcACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACa                                                                                 <  1:3485144/93‑1 (MQ=255)
        gcgcACCAATGAAGGAGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GCTCAGCAGTTGTGTCGGTTGGGTAGACa                                                                                 >  2:1248544/1‑93 (MQ=255)
        gcgcACCAATCAAGGTGGACGGCACCGGCGAAGAGTGCGCCCATTGCATAGTTCTGGAATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACa                                                                                 <  2:5097410/93‑1 (MQ=255)
         cgcACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACAt                                                                                <  1:457753/93‑1 (MQ=255)
          gcACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATc                                                                               >  2:807625/1‑93 (MQ=255)
          gcACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATc                                                                               <  2:4865831/93‑1 (MQ=255)
          gcACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATc                                                                               >  2:6297164/1‑93 (MQ=255)
           cACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCa                                                                              >  1:6092686/1‑93 (MQ=255)
             ccAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAAc                                                                            <  1:489622/93‑1 (MQ=255)
              cAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAAcc                                                                           <  1:1865575/93‑1 (MQ=255)
              cAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAAcc                                                                           >  2:1356014/1‑93 (MQ=255)
              cAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAAcc                                                                           >  2:1745921/1‑93 (MQ=255)
               aaTGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAAccc                                                                          >  2:4873012/1‑93 (MQ=255)
                aTGAAGGTGGACGGCACCGGCGAATAGGGCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACccg                                                                         >  2:5670974/1‑93 (MQ=255)
                 tGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACccgc                                                                        >  1:2113056/1‑93 (MQ=255)
                  gAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACccgcc                                                                       >  2:3805217/1‑93 (MQ=255)
                   aaGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACccgccg                                                                      >  1:3972238/1‑93 (MQ=255)
                    aGGTGGACGACACCGGCGAAGGGGTCGCCCATTGCATGGTGCGGGGATCGAG‑GTTCAGCGGTTGCGGCGGTTGGGTAGACATCACCCCGCCGa                                                                     <  2:329733/93‑1 (MQ=255)
                    aGGGGGCCGGCCCGGGCAAAGCGGCCCCCATTTGAATGGTGCTGGGATCGAA‑GTTCAGCATTTGCGTCGGTGGGGAAGAAACCAACCCGCCGa                                                                     <  2:3240789/93‑1 (MQ=255)
                     ggTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGAt                                                                    >  1:6064030/1‑93 (MQ=255)
                     ggTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGAt                                                                    >  1:4398817/1‑93 (MQ=255)
                     ggTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGAt                                                                    <  2:510948/93‑1 (MQ=255)
                      gTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATa                                                                   >  1:5164547/1‑93 (MQ=255)
                       tGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATaa                                                                  >  1:6290261/1‑93 (MQ=255)
                         gACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATaaaa                                                                >  2:2032580/1‑93 (MQ=255)
                          ggggCACCGGGGAAGAGGTCGCCGATTGTATAGTGCTGGGATCGAA‑GTTCAGCATTTGCGTCGGTGGGGTAGACATCAACCCGCCGATAAAAc                                                               <  2:6249409/91‑1 (MQ=255)
                             gCACCGGCGAAGGGGTCGCCCATTGCAGGGTGCGGGGATCGAA‑GTACAGCAGTTGCGTGGGTGGGGAAGACATCACCCCGCCGATAAAACCAg                                                            <  2:1222455/93‑1 (MQ=255)
                              cACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGc                                                           >  1:1162993/1‑93 (MQ=255)
                               aCCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGcc                                                          >  1:1351026/1‑93 (MQ=255)
                               aCCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGcc                                                          >  1:5108237/1‑93 (MQ=255)
                                 cGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCgg                                                        >  2:5736497/1‑93 (MQ=255)
                                   gCGAAGAGGTCGCCCATTGCATAGTGCTGGGCTCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGa                                                      >  1:2259422/1‑93 (MQ=255)
                                   gCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGa                                                      >  1:5354951/1‑93 (MQ=255)
                                   gCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGGAGACATCAACCCGCCGATAAAACCAGCCGGGa                                                      >  1:3114131/1‑93 (MQ=255)
                                     gAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAAc                                                    >  1:4813521/1‑93 (MQ=255)
                                     gAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAAc                                                    >  2:5300738/1‑93 (MQ=255)
                                      aaGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACg                                                   >  2:3830824/1‑93 (MQ=255)
                                       agagGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGc                                                  >  1:863901/1‑93 (MQ=255)
                                       agagGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGc                                                  >  2:622525/1‑93 (MQ=255)
                                         agGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCa                                                >  1:1495585/1‑93 (MQ=255)
                                           gTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGt                                              >  2:5760199/1‑93 (MQ=255)
                                           gTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGt                                              >  2:5851209/1‑93 (MQ=255)
                                           gTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGt                                              >  1:465997/1‑93 (MQ=255)
                                             cGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGttt                                            >  1:2438742/1‑93 (MQ=255)
                                              gCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTg                                           >  2:5059786/1‑93 (MQ=255)
                                               cccATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGc                                          >  2:1505787/1‑93 (MQ=255)
                                               cccATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGc                                          >  2:442396/1‑93 (MQ=255)
                                               cccATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGc                                          >  1:5889165/1‑93 (MQ=255)
                                                ccATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGcc                                         >  2:2515606/1‑93 (MQ=255)
                                                ccATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGcc                                         >  2:2023301/1‑93 (MQ=255)
                                                ccATTGAATAGTGGGGGGATAGAA‑GTTCAGCTGTTGCGTGGGTTGGGAAGACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGcc                                         <  1:4416384/93‑1 (MQ=255)
                                                caaTTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGcc                                         >  2:3296999/3‑93 (MQ=255)
                                                 cATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCa                                        >  1:87653/1‑93 (MQ=255)
                                                 cATTGAATAGTGCGGGGATGGAA‑GTTCAGCAGTTGCGTCGGTTGGGAAAACACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCa                                        <  2:1423385/93‑1 (MQ=255)
                                                  tttGAATGGTGCT‑GGATTGAATGTTCGGCGTTTGCGTGGGTGGGGAAAACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCAc                                       <  2:4718750/92‑1 (MQ=255)
                                                   ttGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCAcc                                      >  1:5827587/1‑93 (MQ=255)
                                                   ttGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCAcc                                      >  2:4382317/1‑93 (MQ=255)
                                                   ttGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCAcc                                      >  1:3805744/1‑93 (MQ=255)
                                                      cATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGAt                                   >  1:3907275/1‑93 (MQ=255)
                                                       aTAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATa                                  >  1:5984006/1‑93 (MQ=255)
                                                        tAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATag                                 >  2:5307713/1‑93 (MQ=255)
                                                         ggTGCTGGGAGGGAA‑TTCCAGCAGTTGGGTGGGTGGGGGAGACACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATaga                                <  1:3755274/92‑1 (MQ=255)
                                                          gTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATagag                               >  2:2045156/1‑93 (MQ=255)
                                                          gTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATagag                               >  2:5518875/1‑93 (MQ=255)
                                                           tGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGt                              >  1:1670390/1‑93 (MQ=255)
                                                            ggtggaatggaa‑GTTAAGCTGTTGGGTGGGTTGGTAAAACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGtt                             <  2:2151868/91‑1 (MQ=255)
                                                            gCTTGGATCTAA‑GTTCAGCAGTTGCGTAGGGTGGGTAGACATCAACCCTCCGGTAAAACCATCCGTGAACGCCAGTTGGCCACCGATAGAGtt                             >  2:3245499/1‑93 (MQ=255)
                                                              tGGGATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCg                           >  2:4059765/1‑93 (MQ=255)
                                                               gggATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGc                          >  2:4326311/1‑93 (MQ=255)
                                                                 gATCGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCAGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCca                        >  1:4431538/1‑93 (MQ=255)
                                                                  aTCGAA‑GTTCAGCAGTTGCGTCGGTTGGGGAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGGTCGcata                       >  1:5203710/1‑90 (MQ=255)
                                                                    caaa‑GTTCAGCAGTGGCGGCGGTGGGGAAGACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACaa                     <  2:4801735/91‑1 (MQ=255)
                                                                    cGAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACaa                     >  2:2496112/1‑93 (MQ=255)
                                                                     gAA‑GTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACaaa                    >  2:4953407/1‑93 (MQ=255)
                                                                       aggTTCAGCTGTGGCGGGGGTGGGGTAAACCCCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACaaaa                   <  1:5472255/91‑1 (MQ=255)
                                                                       a‑GTACAGCGGTGGCGGGGGTTGGGAAGACATCAGCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAag                  <  1:5994995/93‑1 (MQ=255)
                                                                         gTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAagc                 >  1:1145545/1‑93 (MQ=255)
                                                                          ttCAGCAGTTGCGTGGGTTGGGTAAACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAagca                <  1:2454880/93‑1 (MQ=255)
                                                                          ttCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAagca                >  2:3403475/1‑93 (MQ=255)
                                                                           tCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAagcag               >  2:4856620/1‑93 (MQ=255)
                                                                               cagTTGTGTGGGTGGGATAGACACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCaaa           <  1:745906/93‑1 (MQ=255)
                                                                                agTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAAc          >  1:5124188/1‑93 (MQ=255)
                                                                                 gtggCGTTGGTTGGTTAGACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACa         <  2:6148040/90‑1 (MQ=255)
                                                                                 gTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACa         >  2:2937365/1‑93 (MQ=255)
                                                                                 gTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACa         >  2:1182773/1‑93 (MQ=255)
                                                                                    gCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCg      >  2:2770984/1‑93 (MQ=255)
                                                                                      gTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGa    >  1:1662585/1‑93 (MQ=255)
                                                                                       tCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGaa   >  2:411601/1‑93 (MQ=255)
                                                                                           |                                                                                         
AATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAA‑GTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAAT  >  NC_000913/2505405‑2505584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCA  >  NC_000913/2505402‑2505588
                                                                                             |                                                                                             
AGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGT                                                                                               >  SRR4302533.2394951/1‑94 (MQ=60)
AGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTCGGATCGAAGCTCAGGAGCTGCGTCGGT                                                                                               >  SRR4302533.3953058/1‑94 (MQ=60)
  AAATAATCAGCGCACCAATGAAGGTGGACGGCCCCGGCGAAGAGGTCGCACATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTG                                                                                             <  SRR4302533.3129653/94‑1 (MQ=60)
  AAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTG                                                                                             <  SRR4302533.6151988/94‑1 (MQ=60)
  AAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTG                                                                                             >  SRR4302533.472785/1‑94 (MQ=60)
   AATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGG                                                                                            <  SRR4302533.3333027/94‑1 (MQ=60)
    ATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGCTGCGTCGGTTGGG                                                                                           <  SRR4302533.3424453/94‑1 (MQ=60)
       ATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAG                                                                                        >  SRR4302533.2975737/1‑94 (MQ=60)
         CAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGAC                                                                                      <  SRR4302533.4758466/94‑1 (MQ=60)
          AGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACA                                                                                     <  SRR4302533.1867871/94‑1 (MQ=60)
           GCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACAT                                                                                    <  SRR4302533.3506638/94‑1 (MQ=60)
           GCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACAT                                                                                    <  SRR4302533.1979777/94‑1 (MQ=60)
            CGCACCAATGAAGGTGGATGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATC                                                                                   >  SRR4302533.831132/1‑94 (MQ=60)
            CGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATC                                                                                   <  SRR4302533.460490/94‑1 (MQ=60)
             GCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCA                                                                                  >  SRR4302533.6129502/1‑94 (MQ=60)
                CCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACC                                                                               <  SRR4302533.492550/94‑1 (MQ=60)
                 CAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCC                                                                              <  SRR4302533.2113612/94‑1 (MQ=60)
                 CAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCC                                                                              <  SRR4302533.1876953/94‑1 (MQ=60)
                   ATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGC                                                                            >  SRR4302533.2126090/1‑94 (MQ=60)
                     GAAGGTGGACGGCACCGGCGAAGAGGTCGGCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCG                                                                          >  SRR4302533.3915951/1‑94 (MQ=60)
                     GAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCG                                                                          >  SRR4302533.3996428/1‑94 (MQ=60)
                       AGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCGGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGAT                                                                        >  SRR4302533.1623223/1‑94 (MQ=60)
                       AGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGAT                                                                        >  SRR4302533.4425373/1‑94 (MQ=60)
                       AGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGAT                                                                        >  SRR4302533.6100737/1‑94 (MQ=60)
                       AGGGGGACGGCACCGGCGAAGAGGTCCCCCATTGCATGGTGCGGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCACCCCGCCGAT                                                                        <  SRR4302533.5790596/94‑1 (MQ=60)
                        GGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATA                                                                       >  SRR4302533.5195733/1‑94 (MQ=60)
                         GTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAA                                                                      >  SRR4302533.6327774/1‑94 (MQ=60)
                               GGCGCCGGGGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAATTGCAGCTGTTGCGTGGTTGGGGTAGACATCACCCCGCCGATAAAACCAG                                                                <  SRR4302533.1160846/94‑1 (MQ=44)
                               GGCGCCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGGCGGTTGGGTAGACATCAACCCGCCGATAAAACCAG                                                                <  SRR4302533.3085931/94‑1 (MQ=60)
                                GCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGC                                                               >  SRR4302533.1169969/1‑94 (MQ=60)
                                 CCCCGGCGAGGAGGTCGCCCATGGCATAGTGCTGGAATCGAAGTTCAGCAGTGGCGTGGGTGGGGTAGACATCACCCCGCCGATAAAACCAGCC                                                              <  SRR4302533.714181/94‑1 (MQ=60)
                                 CACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCC                                                              >  SRR4302533.5139102/1‑94 (MQ=60)
                                 CACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCC                                                              >  SRR4302533.1359151/1‑94 (MQ=60)
                                  CCCGGCGAGGAGGCCGCCCATGGCATGGTGCTGGGATCAAATTTCAGCAGTTGCGTGGTTTGGGAAGACACCACCCCGCCGATAAAACCAGCCG                                                             <  SRR4302533.3000268/94‑1 (MQ=21)
                                   cgggggaagaggccgccatTTGCATAGTGCGGGGATCGAAGTGCAGCATTTGCGTGGGTGGGGAAAACATCAACCCGCCGATAAAACCAGCCGG                                                            <  SRR4302533.3793402/75‑1 (MQ=30)
                                   CCGGCGAAGAGGCCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGG                                                            >  SRR4302533.4377872/1‑94 (MQ=60)
                                     GGCGAAGAGGTCGCCCATTGCATAGTGCTGGGCTCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGA                                                          >  SRR4302533.2273399/1‑94 (MQ=60)
                                     GGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGA                                                          >  SRR4302533.5387096/1‑94 (MQ=60)
                                     GGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGGAGACATCAACCCGCCGATAAAACCAGCCGGGA                                                          >  SRR4302533.3133282/1‑94 (MQ=60)
                                      GCGAAGAGCTCCCCATTTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTGGGTTGGGTAGACATCACCCCGCCGATAAAACCAGCCGGGAA                                                         <  SRR4302533.3937314/94‑1 (MQ=60)
                                       gaAAGAGGTCGCCCTTTGCATAGTGCTGGAATCAAGGTTCAGCAGTTGCGTCGGTTGGGTAGACATCACCCCGCCGATAAAACCAGCCGGGAAC                                                        <  SRR4302533.4233874/92‑1 (MQ=60)
                                       CGAAGAGGTCGCCCATTGCATGGTGCTGGGATCGAAGTTCAGCAGTTGCGTGGGTGGGGTAGACATCACCCCGCCGATAAAACCAGCCGGGAAC                                                        <  SRR4302533.1117446/94‑1 (MQ=60)
                                       CGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAAC                                                        >  SRR4302533.4842693/1‑94 (MQ=60)
                                        GAAGAGGTCGCCAATTGAATGGGGCTGGGATAGAAGTTAAGCGGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACG                                                       <  SRR4302533.1409760/94‑1 (MQ=60)
                                         AAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGC                                                      >  SRR4302533.868963/1‑94 (MQ=60)
                                         AAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGC                                                      >  SRR4302533.5134514/1‑94 (MQ=60)
                                           GAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCA                                                    >  SRR4302533.3747667/1‑94 (MQ=60)
                                           GAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCA                                                    >  SRR4302533.1504596/1‑94 (MQ=60)
                                             ggcccCCCATTGAATAGTGCTGGGATCGAAGTTCAGGTGTTGCGTGGTTTGGGTAGACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGT                                                  <  SRR4302533.799134/89‑1 (MQ=60)
                                             GGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGT                                                  >  SRR4302533.468781/1‑94 (MQ=60)
                                             GGTCCCCCATTGCATGGTGTTGGGATCGAATTCCAGCAGTGGCGTCGGTTGGGTAGACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGT                                                  <  SRR4302533.5944161/94‑1 (MQ=60)
                                               TCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTT                                                >  SRR4302533.2453802/1‑94 (MQ=60)
                                                 GCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGC                                              >  SRR4302533.5924746/1‑94 (MQ=60)
                                                   CCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCA                                            >  SRR4302533.88214/1‑94 (MQ=60)
                                                   CCATTGAATAGTGGGGGGATAGAAGTTCAGCTGTTGCGTGGGTTGGGAAGACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCA                                            <  SRR4302533.4443075/94‑1 (MQ=53)
                                                     ATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACC                                          >  SRR4302533.5862799/1‑94 (MQ=60)
                                                     ATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACC                                          >  SRR4302533.3829031/1‑94 (MQ=60)
                                                      TTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCG                                         >  SRR4302533.6140859/1‑94 (MQ=60)
                                                        GCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGAT                                       >  SRR4302533.3931140/1‑94 (MQ=60)
                                                         CATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATA                                      >  SRR4302533.6020246/1‑94 (MQ=60)
                                                          ATAGTGCTGGGATCGAAGTTCAGCGTTTGCGTGGTTTGGGAAGACACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAG                                     <  SRR4302533.4659242/94‑1 (MQ=60)
                                                             GTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGT                                  >  SRR4302533.1680485/1‑94 (MQ=60)
                                                                 TGGGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGC                              >  SRR4302533.1200103/1‑94 (MQ=60)
                                                                   GGATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCAGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCA                            >  SRR4302533.4458328/1‑94 (MQ=60)
                                                                    GATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCAC                           >  SRR4302533.479919/1‑94 (MQ=60)
                                                                    GATCGAAGTTCAGCAGTTGCGTCGGTTGGGGAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGgtcgcata                           >  SRR4302533.5235068/1‑86 (MQ=60)
                                                                     ATCGAAGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACA                          >  SRR4302533.581595/1‑94 (MQ=60)
                                                                         agtTTCAGTAGTTGCGTCGGTGGGGTAGACACCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAG                      <  SRR4302533.5985694/91‑1 (MQ=60)
                                                                          AGTTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGC                     >  SRR4302533.1152383/1‑94 (MQ=60)
                                                                            TTCAGCAGTTGCGTGGGTTGGGTAAACATCACCCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAG                   <  SRR4302533.2470030/94‑1 (MQ=60)
                                                                            TTCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACACACGCAG                   >  SRR4302533.232606/1‑94 (MQ=60)
                                                                             TCAGCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGC                  >  SRR4302533.6096417/1‑94 (MQ=60)
                                                                                GCAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAACAGCAGCAAA               >  SRR4302533.2834781/1‑94 (MQ=60)
                                                                                 CAGTTGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAAC              >  SRR4302533.5155145/1‑94 (MQ=60)
                                                                                     TGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCG          >  SRR4302533.526222/1‑94 (MQ=60)
                                                                                     TGCGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCG          >  SRR4302533.5161071/1‑94 (MQ=60)
                                                                                       CGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGA        >  SRR4302533.4696490/1‑94 (MQ=60)
                                                                                       CGTCGGTTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGA        >  SRR4302533.1672635/1‑94 (MQ=60)
                                                                                           GGTTGGGGAGACATCAGCCCGCCGATAAACCCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGG    <  SRR4302533.656909/94‑1 (MQ=60)
                                                                                             TTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCA  >  SRR4302533.350956/1‑94 (MQ=60)
                                                                                             |                                                                                             
AGAAATAATCAGCGCACCAATGAAGGTGGACGGCACCGGCGAAGAGGTCGCCCATTGCATAGTGCTGGGATCGAAGTTCAGCAGTTGCGTCGGCTGGGTAGACATCAACCCGCCGATAAAACCAGCCGGGAACGCCAGTTTGCCACCGATAGAGTTCGCCACAAAAGCAGCAAACATCGGAATGGCA  >  NC_000913/2505402‑2505588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑