Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,536,994	T→A	S204C (AGC→TGC)	cysM ←	cysteine synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,536,994	0	T	A	91.4%	737.0 / 49.5	223	S204C (AGC→TGC)	cysM	cysteine synthase B
Reads supporting (aligned to +/- strand): ref base T (6/13); new base A (95/108); total (102/121)
Fisher's exact test for biased strand distribution p-value = 2.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 235 total aligned reads displayed.

GATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGTCC  >  NC_000913/2536904‑2537083

  tgaATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGccc                                                                                       >  1:4851378/1‑93 (MQ=255)

  tgaATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGccc                                                                                       >  1:6745447/1‑93 (MQ=255)

  tgaATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGccc                                                                                       <  1:5213918/93‑1 (MQ=255)

    aaTATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCctc                                                                                     <  2:4096036/93‑1 (MQ=255)

    aaTATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCctc                                                                                     <  1:5905679/93‑1 (MQ=255)

     atatCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCctct                                                                                    <  1:7136111/93‑1 (MQ=255)

     atatCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCctct                                                                                    <  1:7710973/93‑1 (MQ=255)

       atCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTc                                                                                  <  2:3227369/93‑1 (MQ=255)

       atCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTc                                                                                  <  1:8535369/93‑1 (MQ=255)

        tCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTcc                                                                                 <  2:660291/93‑1 (MQ=255)

        tCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTcc                                                                                 >  1:1665369/1‑93 (MQ=255)

         ccAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCg                                                                                >  2:8167102/1‑93 (MQ=255)

          cAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCgg                                                                               <  1:3547319/93‑1 (MQ=255)

              aCCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGc                                                                           <  2:5884103/93‑1 (MQ=255)

              aCCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGc                                                                           >  2:3685440/1‑93 (MQ=255)

               ccTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCa                                                                          >  1:4655254/1‑93 (MQ=255)

               ccTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCa                                                                          <  2:6463591/93‑1 (MQ=255)

               ccTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCa                                                                          <  1:1186131/93‑1 (MQ=255)

               ccTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCa                                                                          <  2:6771423/93‑1 (MQ=255)

               ccTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCa                                                                          <  1:1557782/93‑1 (MQ=255)

                cTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAg                                                                         >  1:3282573/1‑93 (MQ=255)

                 tCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAgg                                                                        >  1:3395010/1‑93 (MQ=255)

                 tCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAgg                                                                        >  2:640884/1‑93 (MQ=255)

                  cATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGc                                                                       <  2:3359670/93‑1 (MQ=255)

                   aTCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGcc                                                                      <  2:3480608/93‑1 (MQ=255)

                   aTCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGcc                                                                      >  1:2490586/1‑93 (MQ=255)

                      caccaGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGAc                                                                   <  2:715926/93‑1 (MQ=255)

                         caGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAAt                                                                <  2:8064771/93‑1 (MQ=255)

                           gagaAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATgg                                                              >  1:5366954/1‑93 (MQ=255)

                           gagaAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATgg                                                              <  1:1336470/93‑1 (MQ=255)

                           gagaAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATgg                                                              >  1:6042394/1‑93 (MQ=255)

                           gagaAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATgg                                                              >  1:9709793/1‑93 (MQ=255)

                             gaAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTc                                                            >  2:9236637/1‑93 (MQ=255)

                               aGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCAc                                                          >  2:4328078/1‑93 (MQ=255)

                               aGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCAc                                                          <  1:6369731/93‑1 (MQ=255)

                                gCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCAcc                                                         <  2:1690081/93‑1 (MQ=255)

                                gCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCAcc                                                         <  1:7731361/93‑1 (MQ=255)

                                 cGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCg                                                        <  1:1012752/93‑1 (MQ=255)

                                   ttGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGGCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGt                                                      >  1:6684808/1‑93 (MQ=255)

                                   ttGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGt                                                      >  1:4169422/1‑93 (MQ=255)

                                   ttGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGt                                                      >  1:4061614/1‑93 (MQ=255)

                                    tGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGtt                                                     <  1:7293805/93‑1 (MQ=255)

                                    tGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGtt                                                     <  1:7523373/93‑1 (MQ=255)

                                     gAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGttt                                                    <  1:7933805/93‑1 (MQ=255)

                                      aaaaTCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTg                                                   <  1:5066229/93‑1 (MQ=255)

                                        aaTCCCCGGCAGATATTCCGTAGGCCAGCGGCGCATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGa                                                 <  2:8955518/93‑1 (MQ=255)

                                         aTCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGAt                                                <  1:9373410/93‑1 (MQ=255)

                                          tCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGAtt                                               <  1:6911661/93‑1 (MQ=255)

                                             ccGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGtt                                            <  1:1405286/93‑1 (MQ=255)

                                              cGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTc                                           <  2:7435837/93‑1 (MQ=255)

                                              cGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTc                                           <  1:9318835/93‑1 (MQ=255)

                                              cGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTc                                           <  1:3788967/93‑1 (MQ=255)

                                               ggCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTcg                                          >  1:3369179/1‑93 (MQ=255)

                                               ggCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTcg                                          <  2:9548407/93‑1 (MQ=255)

                                                 cAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTcgcg                                        >  2:4844018/1‑93 (MQ=255)

                                                   gATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCa                                      >  1:4264998/1‑93 (MQ=255)

                                                   gATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCa                                      <  1:2735797/93‑1 (MQ=255)

                                                   gATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCa                                      >  1:3817425/1‑93 (MQ=255)

                                                    atatTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCAt                                     <  1:5619602/93‑1 (MQ=255)

                                                     tatTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATa                                    <  2:9341299/93‑1 (MQ=255)

                                                      ggtCCGTAGGGGAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGGCGACAATGGTCCCCGGTTTGGATTGTTCGCGCATaa                                   <  2:5275004/91‑1 (MQ=255)

                                                      atTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATaa                                   >  2:8361352/1‑93 (MQ=255)

                                                       ttCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATaaa                                  >  1:7381148/1‑93 (MQ=255)

                                                        tCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAg                                 <  2:1646574/93‑1 (MQ=255)

                                                          cGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCg                               <  1:8696909/93‑1 (MQ=255)

                                                          cGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCg                               >  1:9905052/1‑93 (MQ=255)

                                                          cGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCg                               >  2:357718/1‑93 (MQ=255)

                                                          cGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCg                               >  2:3740174/1‑93 (MQ=255)

                                                            tAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTg                             <  2:6882457/93‑1 (MQ=255)

                                                            tAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTg                             <  2:2539075/93‑1 (MQ=255)

                                                               gCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTgaga                          <  2:7614833/93‑1 (MQ=255)

                                                               gCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTgaga                          <  1:9259052/93‑1 (MQ=255)

                                                                ccAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGAc                         <  2:9472125/93‑1 (MQ=255)

                                                                ccAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGAc                         >  1:4324827/1‑93 (MQ=255)

                                                                 cAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACg                        <  2:7793711/93‑1 (MQ=255)

                                                                 cAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACg                        >  2:743029/1‑93 (MQ=255)

                                                                  aGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGc                       >  2:9162138/1‑93 (MQ=255)

                                                                     ggcgAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCgg                    >  1:8751976/1‑93 (MQ=255)

                                                                     ggcgAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCgg                    <  2:1210252/93‑1 (MQ=255)

                                                                     ggcgAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACACCgg                    >  1:5989393/1‑93 (MQ=255)

                                                                      gcgAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCggg                   >  2:3112406/1‑92 (MQ=255)

                                                                      gcgAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGt                   >  2:243612/1‑93 (MQ=255)

                                                                       cgAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTg                  <  2:4869130/93‑1 (MQ=255)

                                                                       cgAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTg                  >  1:2418308/1‑93 (MQ=255)

                                                                       cgAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTg                  >  1:1977340/1‑93 (MQ=255)

                                                                          aTGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATa               >  2:7661938/1‑93 (MQ=255)

                                                                          aTGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATa               <  1:6822358/93‑1 (MQ=255)

                                                                           tGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAg              <  2:2049345/93‑1 (MQ=255)

                                                                           tGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAg              >  2:8711501/1‑93 (MQ=255)

                                                                             ccGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTg            >  1:8284252/1‑93 (MQ=255)

                                                                               gggAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGcc          <  2:4919475/93‑1 (MQ=255)

                                                                               ggaaaTGCTGCAGCCCTCTTCCGGTGGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGcc          <  2:131477/90‑1 (MQ=255)

                                                                                 gAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCgg        >  1:4537840/1‑93 (MQ=255)

                                                                                  aaTGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGgt       <  2:9663018/93‑1 (MQ=255)

                                                                                   aTGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGgtc      >  1:8124033/1‑93 (MQ=255)

                                                                                    agctgcagcCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGgtcg     <  1:9983684/92‑1 (MQ=255)

                                                                                     gctgcagcCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGgtcgt    >  1:9598191/1‑93 (MQ=255)

                                                                                      ctgcagcCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTTCCGgtcgtc   >  1:2864051/1‑93 (MQ=255)

                                                                                       tgcagcCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGTcc  >  1:3055170/1‑93 (MQ=255)

                                                                                       tgcagcCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGTcc  <  2:5840903/93‑1 (MQ=255)

GATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGTCC  >  NC_000913/2536904‑2537083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGTCCCCAT  >  NC_000913/2536902‑2537087

CTGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAG                                                                                              >  SRR4302540.6408976/1‑94 (MQ=60)

CTGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAG                                                                                              >  SRR4302540.8040887/1‑94 (MQ=60)

CTGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAG                                                                                              >  SRR4302540.6638186/1‑94 (MQ=60)

CTGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAG                                                                                              <  SRR4302540.5388469/94‑1 (MQ=60)

CTGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAG                                                                                              <  SRR4302540.5388470/94‑1 (MQ=60)

 TGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGC                                                                                             >  SRR4302540.6619284/1‑94 (MQ=60)

 TGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAACGCCGGGAATGCTGCAGC                                                                                             >  SRR4302540.8785973/1‑94 (MQ=60)

   ATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCC                                                                                           >  SRR4302540.4903364/1‑94 (MQ=60)

   ATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCC                                                                                           >  SRR4302540.6819981/1‑94 (MQ=60)

    TGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCT                                                                                          <  SRR4302540.5270736/94‑1 (MQ=60)

      AATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCT                                                                                        <  SRR4302540.9474088/94‑1 (MQ=60)

      AATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCT                                                                                        <  SRR4302540.5970913/94‑1 (MQ=60)

       ATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTT                                                                                       <  SRR4302540.7215615/94‑1 (MQ=60)

       ATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTT                                                                                       <  SRR4302540.6008843/94‑1 (MQ=60)

       ATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTT                                                                                       <  SRR4302540.1742053/94‑1 (MQ=60)

       ATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTT                                                                                       <  SRR4302540.7799645/94‑1 (MQ=60)

        TATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTC                                                                                      <  SRR4302540.7446433/94‑1 (MQ=60)

        TATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTC                                                                                      >  SRR4302540.8555774/1‑94 (MQ=60)

         ATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCC                                                                                     >  SRR4302540.1683496/1‑94 (MQ=60)

         ATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCC                                                                                     <  SRR4302540.8278351/94‑1 (MQ=60)

         ATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCC                                                                                     <  SRR4302540.8634536/94‑1 (MQ=60)

            CAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGT                                                                                  <  SRR4302540.3586066/94‑1 (MQ=60)

            CAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGT                                                                                  <  SRR4302540.2899470/94‑1 (MQ=60)

              GCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTG                                                                                <  SRR4302540.3352366/94‑1 (MQ=60)

               CACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGC                                                                               >  SRR4302540.9374275/1‑94 (MQ=60)

                ACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCA                                                                              >  SRR4302540.4705074/1‑94 (MQ=60)

                ACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCA                                                                              >  SRR4302540.5450359/1‑94 (MQ=60)

                 CCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTATTCCGGTTGCAG                                                                             >  SRR4302540.1204005/1‑94 (MQ=60)

                 CCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAG                                                                             <  SRR4302540.4266207/94‑1 (MQ=60)

                 CCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAG                                                                             >  SRR4302540.3318451/1‑94 (MQ=60)

                 CCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAG                                                                             <  SRR4302540.2785906/94‑1 (MQ=60)

                 CCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAG                                                                             >  SRR4302540.5846472/1‑94 (MQ=60)

                 CCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAG                                                                             <  SRR4302540.1758562/94‑1 (MQ=60)

                 CCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAG                                                                             <  SRR4302540.1574902/94‑1 (MQ=60)

                 CCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAG                                                                             <  SRR4302540.1198367/94‑1 (MQ=60)

                  CTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGG                                                                            >  SRR4302540.3432043/1‑94 (MQ=60)

                    CATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCC                                                                          >  SRR4302540.5909742/1‑94 (MQ=60)

                    CATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCC                                                                          >  SRR4302540.2517410/1‑94 (MQ=60)

                     ATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCG                                                                         >  SRR4302540.4437839/1‑94 (MQ=60)

                     ATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCG                                                                         >  SRR4302540.428499/1‑94 (MQ=60)

                     ATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCG                                                                         <  SRR4302540.1000125/94‑1 (MQ=60)

                     ATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCACCCCTCTTCCGGTTGCAGGCCG                                                                         >  SRR4302540.7624690/1‑94 (MQ=60)

                       CCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGAC                                                                       >  SRR4302540.756189/1‑94 (MQ=60)

                       CCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGAC                                                                       <  SRR4302540.3847462/94‑1 (MQ=60)

                         ACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAA                                                                     >  SRR4302540.519977/1‑94 (MQ=60)

                           CAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATG                                                                   <  SRR4302540.406831/94‑1 (MQ=60)

                            AGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGG                                                                  >  SRR4302540.5425926/1‑94 (MQ=60)

                            AGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGG                                                                  >  SRR4302540.5425927/1‑94 (MQ=60)

                            AGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGG                                                                  >  SRR4302540.6109505/1‑94 (MQ=60)

                            AGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGG                                                                  >  SRR4302540.9823910/1‑94 (MQ=60)

                            AGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGG                                                                  >  SRR4302540.4170168/1‑94 (MQ=60)

                            AGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGG                                                                  >  SRR4302540.2664963/1‑94 (MQ=60)

                             GAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGT                                                                 <  SRR4302540.1350462/94‑1 (MQ=60)

                                 AGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACC                                                             <  SRR4302540.6440664/94‑1 (MQ=60)

                                  GCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCG                                                            <  SRR4302540.3929067/94‑1 (MQ=60)

                                  GCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCG                                                            <  SRR4302540.7821615/94‑1 (MQ=60)

                                  GCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCG                                                            <  SRR4302540.7821617/94‑1 (MQ=60)

                                  GCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCG                                                            <  SRR4302540.9711137/94‑1 (MQ=60)

                                   CGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGG                                                           <  SRR4302540.1023016/94‑1 (MQ=60)

                                    GTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGGCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGT                                                          >  SRR4302540.6758629/1‑94 (MQ=60)

                                    GTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGT                                                          >  SRR4302540.2446929/1‑94 (MQ=60)

                                    GTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGT                                                          >  SRR4302540.4105402/1‑94 (MQ=60)

                                    GTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGT                                                          >  SRR4302540.4214643/1‑94 (MQ=60)

                                    GTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGT                                                          >  SRR4302540.1363534/1‑94 (MQ=60)

                                     TTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTT                                                         <  SRR4302540.7511009/94‑1 (MQ=60)

                                      TGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTT                                                        <  SRR4302540.7375206/94‑1 (MQ=60)

                                      TGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTT                                                        <  SRR4302540.7607535/94‑1 (MQ=60)

                                       TAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTG                                                       <  SRR4302540.681601/94‑1 (MQ=60)

                                       GAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTG                                                       <  SRR4302540.8026277/94‑1 (MQ=60)

                                        AAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGG                                                      <  SRR4302540.5121006/94‑1 (MQ=60)

                                         AAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCTGACGATGGTCACCGGTTTGGA                                                     >  SRR4302540.4915407/1‑94 (MQ=60)

                                           ATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATT                                                   <  SRR4302540.1004692/94‑1 (MQ=60)

                                           ATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATT                                                   <  SRR4302540.6069000/94‑1 (MQ=60)

                                           ATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATT                                                   <  SRR4302540.9485001/94‑1 (MQ=60)

                                           ATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGAAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTCGATT                                                   >  SRR4302540.5123471/1‑94 (MQ=60)

                                            TCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTG                                                  <  SRR4302540.6988564/94‑1 (MQ=60)

                                            TCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTG                                                  <  SRR4302540.4895457/94‑1 (MQ=60)

                                               CCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTC                                               <  SRR4302540.1420419/94‑1 (MQ=60)

                                               CCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTC                                               <  SRR4302540.1420420/94‑1 (MQ=60)

                                               CCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGCGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTC                                               <  SRR4302540.1420421/94‑1 (MQ=60)

                                                CGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCG                                              <  SRR4302540.9430032/94‑1 (MQ=60)

                                                CGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCG                                              >  SRR4302540.3406021/1‑94 (MQ=60)

                                                CGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCG                                              <  SRR4302540.3830281/94‑1 (MQ=60)

                                                  GCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCG                                            <  SRR4302540.7228314/94‑1 (MQ=60)

                                                    AGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCA                                          >  SRR4302540.4311383/1‑94 (MQ=60)

                                                    AGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTCGCATTGTTCGCGCA                                          >  SRR4302540.1642793/1‑94 (MQ=60)

                                                    AGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCA                                          >  SRR4302540.3858987/1‑94 (MQ=60)

                                                     GATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCAT                                         <  SRR4302540.2765328/94‑1 (MQ=60)

                                                      ATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATA                                        <  SRR4302540.5681434/94‑1 (MQ=60)

                                                        ATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAA                                      >  SRR4302540.7463397/1‑94 (MQ=60)

                                                          TCCGTAGGCCCGGGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGC                                    <  SRR4302540.3582064/94‑1 (MQ=60)

                                                           CCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCG                                   >  SRR4302540.10020771/1‑94 (MQ=60)

                                                            CGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGT                                  <  SRR4302540.8797870/94‑1 (MQ=60)

                                                            CGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGT                                  >  SRR4302540.7224108/1‑94 (MQ=60)

                                                            CGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGT                                  <  SRR4302540.8797871/94‑1 (MQ=60)

                                                             GTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTG                                 >  SRR4302540.9626473/1‑94 (MQ=60)

                                                               AGGGCAGCGGCGAATCCCGGGAATGCTGCGGCCCTCTTCCGGTTGCAGGCCGACAAGGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAG                               <  SRR4302540.10072395/94‑1 (MQ=60)

                                                               AGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAG                               <  SRR4302540.10072394/94‑1 (MQ=60)

                                                               AGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCATCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAG                               <  SRR4302540.9784394/94‑1 (MQ=60)

                                                                GGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGA                              >  SRR4302540.4313739/1‑94 (MQ=60)

                                                                GCCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGA                              <  SRR4302540.6393725/94‑1 (MQ=60)

                                                                 GCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGAC                             <  SRR4302540.7205733/94‑1 (MQ=60)

                                                                 GCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGAC                             <  SRR4302540.9369828/94‑1 (MQ=60)

                                                                 GCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGAC                             <  SRR4302540.7808337/94‑1 (MQ=60)

                                                                 GCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGAC                             >  SRR4302540.4371916/1‑94 (MQ=60)

                                                                 GCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGAC                             <  SRR4302540.4656450/94‑1 (MQ=60)

                                                                   CAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGC                           >  SRR4302540.6848687/1‑94 (MQ=60)

                                                                   CAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGC                           >  SRR4302540.6848685/1‑94 (MQ=60)

                                                                    AGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCC                          >  SRR4302540.2655463/1‑94 (MQ=60)

                                                                     GCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCG                         <  SRR4302540.7847594/94‑1 (MQ=60)

                                                                     GCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCG                         <  SRR4302540.8105877/94‑1 (MQ=60)

                                                                      CGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGG                        >  SRR4302540.8853517/1‑94 (MQ=60)

                                                                      CGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGG                        >  SRR4302540.8605362/1‑94 (MQ=60)

                                                                      CGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACACCGG                        >  SRR4302540.6055736/1‑94 (MQ=60)

                                                                        GCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACCATGGTCCCCGGTTTGGATTGTTCGCGCATAAAGCGAGAGATGCCGGTG                      >  SRR4302540.6227462/1‑94 (MQ=60)

                                                                        GCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTG                      >  SRR4302540.1998790/1‑94 (MQ=60)

                                                                        GCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTG                      >  SRR4302540.2444372/1‑94 (MQ=60)

                                                                          GAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGAT                    <  SRR4302540.3425382/94‑1 (MQ=60)

                                                                          GAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGAT                    >  SRR4302540.3647243/1‑94 (MQ=60)

                                                                            ATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAG                  <  SRR4302540.6897887/94‑1 (MQ=60)

                                                                             TGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGT                 <  SRR4302540.5131738/94‑1 (MQ=60)

                                                                             TGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGT                 >  SRR4302540.1117377/1‑94 (MQ=60)

                                                                              GCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTG                >  SRR4302540.8380473/1‑94 (MQ=60)

                                                                               CCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGC               >  SRR4302540.345120/1‑94 (MQ=60)

                                                                               CCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGC               >  SRR4302540.9318322/1‑94 (MQ=60)

                                                                                CGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCC              >  SRR4302540.316012/1‑94 (MQ=60)

                                                                                CGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCC              >  SRR4302540.316011/1‑94 (MQ=60)

                                                                                CGGGAATGCCGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCC              <  SRR4302540.466200/94‑1 (MQ=60)

                                                                                  GGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGG            >  SRR4302540.4586958/1‑94 (MQ=60)

                                                                                    AATGCTGCTGCCCTCTTCCGGTTGCAGGACGACAATGGTCACCAGGTTGGATTGGTCGCGCATAAAACGTGAGACGACGGTGATAGTGCCGGTG          >  SRR4302540.1131684/1‑94 (MQ=60)

                                                                                    AATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTC          >  SRR4302540.8218324/1‑94 (MQ=60)

                                                                                    AATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTC          <  SRR4302540.9020638/94‑1 (MQ=60)

                                                                                     ATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCG         >  SRR4302540.6973947/1‑94 (MQ=60)

                                                                                     ATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCG         >  SRR4302540.2065678/1‑94 (MQ=60)

                                                                                      TGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGT        >  SRR4302540.9711435/1‑94 (MQ=60)

                                                                                      AGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGT        <  SRR4302540.10100085/94‑1 (MQ=60)

                                                                                       GCTGCAGCCCTCTTCCGGTTGCAGGCCGCCAATGGCCACCGGTTTGGAGTGTTCGCGCATCACGCGTGAGACGGCGGTGctgtgtcgggtcgtc       >  SRR4302540.2895119/1‑79 (MQ=49)

                                                                                       GCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTTCCGGTCGTC       >  SRR4302540.2895120/1‑94 (MQ=60)

                                                                                        CTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGTCC      >  SRR4302540.3088691/1‑94 (MQ=60)

                                                                                           CAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGTCCCCA   <  SRR4302540.5617000/94‑1 (MQ=60)

                                                                                            AGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGTCCCCAT  <  SRR4302540.1988461/94‑1 (MQ=60)

                                                                                            AGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGTCCCCAT  <  SRR4302540.3843238/94‑1 (MQ=60)

CTGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGTCCCCAT  >  NC_000913/2536902‑2537087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`