Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,536,994	T→A	S204C (AGC→TGC)	cysM ←	cysteine synthase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,536,994	0	T	A	100.0%	762.2 / NA	205	S204C (AGC→TGC)	cysM	cysteine synthase B
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (85/120); total (85/120)

Only 100 of 225 total aligned reads displayed.

GATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGTCC  >  NC_000913/2536904‑2537083

gatgaATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAAtgctgcagc                                                                                         <  1:567166/93‑1 (MQ=255)

gatgaATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAAtgctgcagc                                                                                         >  1:2623485/1‑93 (MQ=255)

gatgaATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAAtgctgcagc                                                                                         <  1:961098/93‑1 (MQ=255)

gatgaATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAAtgctgcagc                                                                                         >  1:1904897/1‑93 (MQ=255)

  tgaATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGccc                                                                                       <  2:1604744/93‑1 (MQ=255)

  tgaATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGccc                                                                                       <  2:2068884/93‑1 (MQ=255)

   gaATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCct                                                                                      <  2:681093/93‑1 (MQ=255)

   gaATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCct                                                                                      >  2:4405963/1‑93 (MQ=255)

   gaATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCct                                                                                      <  2:519858/93‑1 (MQ=255)

     atatCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCctct                                                                                    <  1:796034/93‑1 (MQ=255)

     atatCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCctct                                                                                    <  2:1297565/93‑1 (MQ=255)

        tCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTcc                                                                                 <  2:4988147/93‑1 (MQ=255)

         ccAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCg                                                                                <  1:2030935/93‑1 (MQ=255)

         ccAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCg                                                                                >  2:3621241/1‑93 (MQ=255)

         ccAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCg                                                                                <  2:2751095/93‑1 (MQ=255)

           aGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGt                                                                              <  2:1534406/93‑1 (MQ=255)

           aGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGt                                                                              >  2:6205/1‑93 (MQ=255)

               ccTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCa                                                                          <  1:6078932/93‑1 (MQ=255)

               ccTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCa                                                                          >  1:5197656/1‑93 (MQ=255)

                cTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCATATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAg                                                                         <  2:5298440/93‑1 (MQ=255)

                cTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAg                                                                         >  1:3157894/1‑93 (MQ=255)

                 tCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAgg                                                                        >  2:3156049/1‑93 (MQ=255)

                 tCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAgg                                                                        <  1:3906547/93‑1 (MQ=255)

                  cATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGc                                                                       <  2:5382841/93‑1 (MQ=255)

                    tCCACCAGAGAAGCGTTGAAAATCAACGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCGGATTGCAGGCCg                                                                     >  2:2603541/1‑93 (MQ=255)

                       accaGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACa                                                                  >  2:2952709/1‑93 (MQ=255)

                        ccaGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACaa                                                                 >  1:5710981/1‑93 (MQ=255)

                         caGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAAt                                                                <  2:2330201/93‑1 (MQ=255)

                         caGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAAt                                                                <  2:970617/93‑1 (MQ=255)

                         caGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCACGCCGACAAt                                                                >  1:33501/1‑93 (MQ=255)

                          agagAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATg                                                               >  1:24308/1‑93 (MQ=255)

                          agagAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATg                                                               <  2:1923916/93‑1 (MQ=255)

                           gagaAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATgg                                                              <  2:2428047/93‑1 (MQ=255)

                            agaAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGt                                                             <  2:3407788/93‑1 (MQ=255)

                             gaAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTc                                                            >  2:2834596/1‑93 (MQ=255)

                             gaAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGGTGCAGGCCGACAATGGTc                                                            >  2:782443/1‑93 (MQ=255)

                              aaGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCa                                                           <  2:5691929/93‑1 (MQ=255)

                              aaGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCa                                                           <  1:1600986/93‑1 (MQ=255)

                               aGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCAc                                                          >  2:4771044/1‑93 (MQ=255)

                               aGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCAc                                                          >  2:2721654/1‑93 (MQ=255)

                               aGCGTTGAAAATCCCCGGCAGATATTCCGTAAGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCCGGTGCATGCCGACTATGGTCAc                                                          >  2:5040814/1‑93 (MQ=255)

                                gCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCAcc                                                         <  1:69536/93‑1 (MQ=255)

                                    tGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGtt                                                     <  2:1232085/93‑1 (MQ=255)

                                     gAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGttt                                                    <  1:4457176/93‑1 (MQ=255)

                                     gAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGttt                                                    <  1:2784860/93‑1 (MQ=255)

                                      aaaaTCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTg                                                   <  1:2564772/93‑1 (MQ=255)

                                      aaaaTCCCCGGCAGAAATTCCGTAGGCCAGCGGCGAATGCCGGCGATGATGCAGCCCTCTTCCCGCTGCAGGCCGAGAATGGGCACCGGGTTg                                                   >  2:5233612/1‑93 (MQ=255)

                                       aaaTCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTgg                                                  <  1:3770346/93‑1 (MQ=255)

                                        aaTCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGa                                                 <  1:225666/93‑1 (MQ=255)

                                            cccGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGt                                             <  2:140192/93‑1 (MQ=255)

                                            cccGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGt                                             >  1:1848900/1‑93 (MQ=255)

                                             ctggCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGtt                                            >  2:4041097/3‑93 (MQ=255)

                                              cGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTc                                           <  1:4823696/93‑1 (MQ=255)

                                                gCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTcgc                                         <  1:341052/93‑1 (MQ=255)

                                                gCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTcgc                                         >  1:1247131/1‑93 (MQ=255)

                                                  aGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCgcgc                                       >  2:608042/1‑93 (MQ=255)

                                                  aGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCgcgc                                       >  1:2751525/1‑93 (MQ=255)

                                                   gATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCa                                      <  2:2003550/93‑1 (MQ=255)

                                                   gATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCa                                      >  2:3929693/1‑93 (MQ=255)

                                                   gATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCa                                      >  1:1524982/1‑93 (MQ=255)

                                                   gATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCa                                      <  1:3966904/93‑1 (MQ=255)

                                                   gATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCa                                      >  1:5916913/1‑93 (MQ=255)

                                                   gATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCa                                      >  1:4485636/1‑93 (MQ=255)

                                                   gATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCa                                      <  1:3646163/93‑1 (MQ=255)

                                                   gATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCa                                      >  2:3538868/1‑93 (MQ=255)

                                                      atTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATaa                                   <  2:5851105/93‑1 (MQ=255)

                                                       ttCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATaag                                  >  1:5037785/1‑92 (MQ=255)

                                                       ttCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATaaa                                  >  2:2284500/1‑93 (MQ=255)

                                                       ttCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATaaa                                  <  2:1320105/93‑1 (MQ=255)

                                                          cGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCg                               <  1:4325583/93‑1 (MQ=255)

                                                          cGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCg                               >  2:4945953/1‑93 (MQ=255)

                                                           gTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGt                              <  1:4114877/93‑1 (MQ=255)

                                                           gTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGt                              <  2:3241435/93‑1 (MQ=255)

                                                           gTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGt                              <  1:2122531/93‑1 (MQ=255)

                                                           gTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGt                              >  1:5449930/1‑93 (MQ=255)

                                                           gTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTAGCGCATAAAGCGt                              >  1:660416/1‑93 (MQ=255)

                                                            tAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTg                             >  1:6207448/1‑93 (MQ=255)

                                                            tAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTg                             <  1:3729591/93‑1 (MQ=255)

                                                                   gcggcgAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGcc                      <  2:3015611/93‑1 (MQ=255)

                                                                   gaggcgAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGATTGGATTGTTCGCGCATAAAGCGTGAGACGcc                      >  1:1238139/3‑93 (MQ=255)

                                                                     ggcgAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCgg                    <  1:6140120/93‑1 (MQ=255)

                                                                      gcgAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGt                   >  1:4167738/1‑93 (MQ=255)

                                                                        gAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGa                 >  1:564072/1‑93 (MQ=255)

                                                                        gAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACCACGGTGa                 >  2:4680246/1‑93 (MQ=255)

                                                                          aTGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATa               <  1:1935091/93‑1 (MQ=255)

                                                                           tGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAg              <  2:937800/93‑1 (MQ=255)

                                                                           tGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAg              >  1:2099396/1‑93 (MQ=255)

                                                                            gCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGt             <  1:169092/93‑1 (MQ=255)

                                                                            gCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGt             >  1:4719243/1‑93 (MQ=255)

                                                                             ccGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTg            <  1:2019795/93‑1 (MQ=255)

                                                                              cGGGAATGCTGCAGCCCTCTTCCGGTTACAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGAGTGAGACGCGCGTGATAGtac           >  2:5597989/1‑91 (MQ=255)

                                                                               gggAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGcc          >  2:6249281/1‑93 (MQ=255)

                                                                                ggAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCg         >  2:3417363/1‑93 (MQ=255)

                                                                                  aaTGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGgt       <  2:5761177/93‑1 (MQ=255)

                                                                                  aaTGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGgt       <  2:1107811/93‑1 (MQ=255)

                                                                                  aaTGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGgt       <  1:2892452/93‑1 (MQ=255)

                                                                                  aaTGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGgt       <  2:3765383/93‑1 (MQ=255)

                                                                                  aaTGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGgt       <  2:4480342/93‑1 (MQ=255)

                                                                                      ctgcagcCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGgtcgtc   <  2:853058/93‑1 (MQ=255)

                                                                                       tgcagcCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGTcc  >  2:4627388/1‑93 (MQ=255)

GATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGTCC  >  NC_000913/2536904‑2537083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCTGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGTCCCCAT  >  NC_000913/2536901‑2537087

GCTGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCA                                                                                               >  SRR4302533.1776516/1‑94 (MQ=60)

  TGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGC                                                                                             >  SRR4302533.2639586/1‑94 (MQ=60)

  TGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGC                                                                                             >  SRR4302533.1916519/1‑94 (MQ=60)

   GATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCC                                                                                            <  SRR4302533.966744/94‑1 (MQ=60)

   GATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCC                                                                                            <  SRR4302533.570523/94‑1 (MQ=60)

   GATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCC                                                                                            <  SRR4302533.1922501/94‑1 (MQ=60)

      GAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTC                                                                                         <  SRR4302533.5549517/94‑1 (MQ=60)

      GAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTC                                                                                         >  SRR4302533.166772/1‑94 (MQ=60)

        ATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTT                                                                                       <  SRR4302533.800716/94‑1 (MQ=60)

        ATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTT                                                                                       <  SRR4302533.2024567/94‑1 (MQ=60)

        ATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTT                                                                                       <  SRR4302533.4435431/94‑1 (MQ=60)

          ATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCC                                                                                     <  SRR4302533.2697799/94‑1 (MQ=60)

          ATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCC                                                                                     >  SRR4302533.3380754/1‑94 (MQ=60)

            CCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGG                                                                                   <  SRR4302533.2043468/94‑1 (MQ=60)

             CAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGT                                                                                  >  SRR4302533.203754/1‑94 (MQ=60)

              AGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTT                                                                                 >  SRR4302533.4151547/1‑94 (MQ=60)

              AGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTT                                                                                 >  SRR4302533.4740709/1‑94 (MQ=60)

                 ACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCA                                                                              <  SRR4302533.2597874/94‑1 (MQ=60)

                 ACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCA                                                                              >  SRR4302533.5229000/1‑94 (MQ=60)

                 ACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCA                                                                              >  SRR4302533.2128131/1‑94 (MQ=60)

                 ACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCA                                                                              <  SRR4302533.1646786/94‑1 (MQ=60)

                  CCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAG                                                                             >  SRR4302533.3177328/1‑94 (MQ=60)

                  CCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAG                                                                             >  SRR4302533.4680225/1‑94 (MQ=60)

                  CCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAG                                                                             <  SRR4302533.6115698/94‑1 (MQ=60)

                  CCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAG                                                                             >  SRR4302533.6291615/1‑94 (MQ=60)

                    TCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGC                                                                           <  SRR4302533.3930410/94‑1 (MQ=60)

                    TCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGC                                                                           >  SRR4302533.2510688/1‑94 (MQ=60)

                     CATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCC                                                                          <  SRR4302533.4213946/94‑1 (MQ=60)

                     CATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCC                                                                          <  SRR4302533.2801123/94‑1 (MQ=60)

                         CACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACA                                                                      >  SRR4302533.2679902/1‑94 (MQ=60)

                          ACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAA                                                                     >  SRR4302533.5745478/1‑94 (MQ=60)

                          ACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAA                                                                     >  SRR4302533.5683660/1‑94 (MQ=60)

                           CCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAAT                                                                    <  SRR4302533.5123820/94‑1 (MQ=60)

                           CCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCACGCCGACAAT                                                                    >  SRR4302533.33729/1‑94 (MQ=60)

                            CAGAGAAGCGTTGAAAATCCCCGGCAGATTTTCCGTAGGCCAGAGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGGAGGACGACCATG                                                                   >  SRR4302533.4496314/1‑94 (MQ=60)

                            CAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATG                                                                   >  SRR4302533.24469/1‑94 (MQ=60)

                               AGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTC                                                                >  SRR4302533.6208747/1‑94 (MQ=60)

                                GAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCA                                                               <  SRR4302533.3283828/94‑1 (MQ=60)

                                 AAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCAC                                                              <  SRR4302533.1610676/94‑1 (MQ=60)

                                   GCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCG                                                            <  SRR4302533.5028305/94‑1 (MQ=60)

                                   GCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCG                                                            <  SRR4302533.69990/94‑1 (MQ=60)

                                    CGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGG                                                           >  SRR4302533.57197/1‑94 (MQ=60)

                                     GTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGT                                                          >  SRR4302533.73713/1‑94 (MQ=60)

                                       TGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTT                                                        >  SRR4302533.3913045/1‑94 (MQ=60)

                                        GAAAATCCCTGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTG                                                       >  SRR4302533.662554/1‑94 (MQ=60)

                                        GAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTG                                                       <  SRR4302533.2801971/94‑1 (MQ=60)

                                        GAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTG                                                       <  SRR4302533.4484129/94‑1 (MQ=60)

                                         AAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGG                                                      <  SRR4302533.2580554/94‑1 (MQ=60)

                                          AAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGA                                                     >  SRR4302533.5359529/1‑94 (MQ=60)

                                          AAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGA                                                     <  SRR4302533.3793462/94‑1 (MQ=60)

                                           AATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGAT                                                    >  SRR4302533.1958945/1‑94 (MQ=60)

                                           AATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGAT                                                    <  SRR4302533.227073/94‑1 (MQ=60)

                                            ATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATT                                                   >  SRR4302533.2955397/1‑94 (MQ=60)

                                             TCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTG                                                  >  SRR4302533.2376759/1‑94 (MQ=60)

                                              CCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGT                                                 >  SRR4302533.1860177/1‑94 (MQ=60)

                                                CCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTC                                               <  SRR4302533.5825384/94‑1 (MQ=60)

                                                 CGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCG                                              <  SRR4302533.4852917/94‑1 (MQ=60)

                                                 CGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCG                                              <  SRR4302533.3758270/94‑1 (MQ=60)

                                                  GGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGC                                             >  SRR4302533.1254579/1‑94 (MQ=60)

                                                  GGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGC                                             <  SRR4302533.3765334/94‑1 (MQ=60)

                                                   GCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCG                                            <  SRR4302533.343136/94‑1 (MQ=60)

                                                   GCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCG                                            <  SRR4302533.1865584/94‑1 (MQ=60)

                                                    CAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGC                                           >  SRR4302533.2768396/1‑94 (MQ=60)

                                                    CAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGC                                           <  SRR4302533.688165/94‑1 (MQ=60)

                                                     AGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCA                                          >  SRR4302533.4512793/1‑94 (MQ=60)

                                                     AGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCA                                          >  SRR4302533.5952673/1‑94 (MQ=60)

                                                     AGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCA                                          >  SRR4302533.1534162/1‑94 (MQ=60)

                                                     AGATATTCCGTAGGCCAGCGGCGAATGCAGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCA                                          <  SRR4302533.6096339/94‑1 (MQ=60)

                                                      GATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCAT                                         <  SRR4302533.3991068/94‑1 (MQ=60)

                                                      GATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCAT                                         <  SRR4302533.3668581/94‑1 (MQ=60)

                                                         ATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAG                                      >  SRR4302533.5068291/1‑94 (MQ=60)

                                                         ATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAA                                      <  SRR4302533.218610/94‑1 (MQ=60)

                                                          TTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAG                                     <  SRR4302533.1340737/94‑1 (MQ=60)

                                                            CCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCG                                   >  SRR4302533.5380510/1‑94 (MQ=60)

                                                             CGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGT                                  >  SRR4302533.5482759/1‑94 (MQ=60)

                                                             CGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGT                                  <  SRR4302533.4351642/94‑1 (MQ=60)

                                                             CGTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTAGCGCATAAAGCGT                                  >  SRR4302533.664306/1‑94 (MQ=60)

                                                              GTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTG                                 <  SRR4302533.2135613/94‑1 (MQ=60)

                                                              GTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTG                                 <  SRR4302533.4139880/94‑1 (MQ=60)

                                                              GTAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTG                                 >  SRR4302533.6244675/1‑94 (MQ=60)

                                                               TAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGA                                <  SRR4302533.1247454/94‑1 (MQ=60)

                                                               TAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGA                                <  SRR4302533.3752470/94‑1 (MQ=60)

                                                               TAGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGA                                <  SRR4302533.3930630/94‑1 (MQ=60)

                                                                AGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAG                               <  SRR4302533.3642309/94‑1 (MQ=60)

                                                                AGGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAG                               <  SRR4302533.4027630/94‑1 (MQ=60)

                                                                 GGCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGA                              >  SRR4302533.582255/1‑94 (MQ=60)

                                                                  GCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGAC                             <  SRR4302533.1760389/94‑1 (MQ=60)

                                                                  GCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGAC                             <  SRR4302533.2870333/94‑1 (MQ=60)

                                                                  GCCAGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGAC                             >  SRR4302533.4765579/1‑94 (MQ=60)

                                                                     AGCGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACCCC                          <  SRR4302533.6125136/94‑1 (MQ=60)

                                                                     AGAGGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGATTGGATTGTTCGCGCATAAAGCGTGAGACGCC                          >  SRR4302533.1245536/1‑94 (MQ=60)

                                                                        GGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGT                       >  SRR4302533.4192995/1‑94 (MQ=60)

                                                                        GGCGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGT                       <  SRR4302533.6177102/94‑1 (MQ=60)

                                                                          CGAATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGA                     >  SRR4302533.567413/1‑94 (MQ=60)

                                                                             ATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAG                  <  SRR4302533.1946896/94‑1 (MQ=60)

                                                                             ATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAG                  >  SRR4302533.2112348/1‑94 (MQ=60)

                                                                             ATGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAG                  >  SRR4302533.2242607/1‑94 (MQ=60)

                                                                              TGCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGT                 >  SRR4302533.4747859/1‑94 (MQ=60)

                                                                               GCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTG                <  SRR4302533.1574546/94‑1 (MQ=60)

                                                                               GCCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTG                <  SRR4302533.170160/94‑1 (MQ=60)

                                                                                CCGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGC               <  SRR4302533.2032236/94‑1 (MQ=60)

                                                                                 CGGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCC              <  SRR4302533.3236731/94‑1 (MQ=60)

                                                                                 CGGGAATGCTGCAGCCCNCTTCCGGTTGCAGGCCGACAATGGTCACCGNTTNGGNTTGTTCGCGCATAAANNNTNAGACGCCGngnannntnnc              >  SRR4302533.4722148/1‑83 (MQ=60)

                                                                                  GGGAATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCG             <  SRR4302533.3744511/94‑1 (MQ=60)

                                                                                     AATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTC          <  SRR4302533.2910232/94‑1 (MQ=60)

                                                                                      ATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCG         >  SRR4302533.970676/1‑94 (MQ=60)

                                                                                      ATGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCG         <  SRR4302533.4160417/94‑1 (MQ=60)

                                                                                       TGCTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGT        <  SRR4302533.92111/94‑1 (MQ=60)

                                                                                         CTGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGTCC      >  SRR4302533.2096989/1‑94 (MQ=60)

                                                                                          TGCAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGTCCC     >  SRR4302533.2293448/1‑94 (MQ=60)

                                                                                            CAGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGTCCCCA   >  SRR4302533.2283294/1‑94 (MQ=60)

                                                                                             AGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGTCCCCAT  <  SRR4302533.1746261/94‑1 (MQ=60)

GCTGATGAATATCCAGCACCTCATCCACCAGAGAAGCGTTGAAAATCCCCGGCAGATATTCCGTAGGCCAGCGGCGAATGCCGGGAATGCTGCTGCCCTCTTCCGGTTGCAGGCCGACAATGGTCACCGGTTTGGATTGTTCGCGCATAAAGCGTGAGACGCCGGTGATAGTGCCGGTCGTCCCCAT  >  NC_000913/2536901‑2537087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`