Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,552,312 T→C V297V (GTA→GTG)  yfeG ← putative ARAC‑type regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,552,3120TC98.9% 814.3 / ‑2.1 261V297V (GTA→GTG) yfeGputative ARAC‑type regulatory protein
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (137/121);  total (139/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

Only 100 of 274 total aligned reads displayed.

GGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGAC  >  NC_000913/2552222‑2552401
                                                                                          |                                                                                          
ggCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCAc                                                                                          <  1:4586144/93‑1 (MQ=255)
ggCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCAc                                                                                          <  2:3511089/93‑1 (MQ=255)
 gCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACg                                                                                         >  2:6196252/1‑93 (MQ=255)
 gCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACg                                                                                         <  2:4485588/93‑1 (MQ=255)
  cAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACgg                                                                                        <  2:1053300/93‑1 (MQ=255)
    aaTTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCg                                                                                      >  1:1492404/1‑93 (MQ=255)
    aaTTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCg                                                                                      >  2:6962513/1‑93 (MQ=255)
     aTTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCATTTCGCGGCGCACGGCGt                                                                                     >  1:8374405/1‑93 (MQ=255)
     aTTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGt                                                                                     >  2:2564530/1‑93 (MQ=255)
      ttGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGtt                                                                                    <  2:242752/93‑1 (MQ=255)
         ccccAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAg                                                                                 >  2:1211819/1‑93 (MQ=255)
            cAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCg                                                                              >  1:6748298/1‑93 (MQ=255)
             aGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGa                                                                             <  2:677410/93‑1 (MQ=255)
             aGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGa                                                                             >  2:5252780/1‑93 (MQ=255)
              gATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGaa                                                                            <  2:8721662/93‑1 (MQ=255)
              gATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGAACGGCGTTCAGGCGag                                                                            >  1:3513460/1‑92 (MQ=255)
                 gCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGc                                                                         <  2:9208615/93‑1 (MQ=255)
                 gCCAGAAACCCCACTGGATGGCGGCGTCTTTTAACGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGc                                                                         <  1:6983760/93‑1 (MQ=255)
                    aGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCCGTTCGCGGCGCACGGCGTTCAGGCGAATGCGtt                                                                      >  1:9704772/1‑93 (MQ=255)
                    aGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGtt                                                                      >  1:3063566/1‑93 (MQ=255)
                    aGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGtt                                                                      <  1:2076157/93‑1 (MQ=255)
                     gAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGttt                                                                     <  2:9315296/93‑1 (MQ=255)
                      aaTCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTc                                                                    <  1:9228792/93‑1 (MQ=255)
                         ccccACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGc                                                                 <  2:7639221/93‑1 (MQ=255)
                           ccACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCa                                                               >  1:49680/1‑93 (MQ=255)
                           ccACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCa                                                               <  1:5224972/93‑1 (MQ=255)
                            cACTGCATGGCGGCGTCTTTTACCGTCATACTTGGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑c                                                             <  2:1485687/93‑1 (MQ=255)
                              cTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑cgc                                                           >  2:9473155/1‑93 (MQ=255)
                               tGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑cgcg                                                          >  2:4398625/1‑93 (MQ=255)
                               tGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑cgcg                                                          >  2:102253/1‑93 (MQ=255)
                                gCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGt                                                         <  2:7210827/93‑1 (MQ=255)
                                gCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGt                                                         <  2:5600266/93‑1 (MQ=255)
                                 cATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGtt                                                        <  2:8617799/93‑1 (MQ=255)
                                 cATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGtt                                                        <  1:3026776/93‑1 (MQ=255)
                                 cATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGtt                                                        <  2:1759884/93‑1 (MQ=255)
                                  ctGGCGGCGTCTTTTACCGTCATACTTTGCAACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTc                                                       <  1:6927262/92‑1 (MQ=255)
                                  aTGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTc                                                       >  2:3413886/1‑93 (MQ=255)
                                   tGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCg                                                      >  1:2641970/1‑93 (MQ=255)
                                    ggcggcGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCgg                                                     <  1:2752798/93‑1 (MQ=255)
                                     gcggcgTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCggg                                                    <  2:3020334/93‑1 (MQ=255)
                                      cggcgTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGc                                                   <  1:8325415/93‑1 (MQ=255)
                                         cgTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCaa                                                <  1:5955465/93‑1 (MQ=255)
                                         cgTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCaa                                                <  1:1701628/93‑1 (MQ=255)
                                         cgTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCaa                                                <  2:6986384/93‑1 (MQ=255)
                                          gTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAAt                                               >  1:9441193/1‑93 (MQ=255)
                                           tCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATg                                              >  2:5098814/1‑93 (MQ=255)
                                           tCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATg                                              >  1:5401382/1‑93 (MQ=255)
                                           tCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATg                                              >  1:2328094/1‑93 (MQ=255)
                                             ttttACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGcc                                            >  2:3839808/1‑93 (MQ=255)
                                             ttttACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGcc                                            >  2:7164792/1‑93 (MQ=255)
                                              tttACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCt                                           >  2:8674718/1‑93 (MQ=255)
                                              tttACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCt                                           >  2:8577913/1‑93 (MQ=255)
                                              tttACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCt                                           >  2:7371854/1‑93 (MQ=255)
                                               ttACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTa                                          >  2:8808528/1‑93 (MQ=255)
                                               ttACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTa                                          <  1:9176334/93‑1 (MQ=255)
                                                tACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTaa                                         <  1:1407836/93‑1 (MQ=255)
                                                 aCCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTaaa                                        <  1:443258/93‑1 (MQ=255)
                                                   cGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAAt                                      >  2:649413/1‑93 (MQ=255)
                                                     tCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAg                                    >  1:7626900/1‑93 (MQ=255)
                                                     tCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAg                                    >  1:5494418/1‑93 (MQ=255)
                                                     tCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAg                                    >  2:6693913/1‑93 (MQ=255)
                                                     tCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAg                                    >  1:6581095/1‑93 (MQ=255)
                                                     tCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAg                                    >  2:8139611/1‑93 (MQ=255)
                                                     tCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAg                                    >  1:4757916/1‑93 (MQ=255)
                                                      cATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGc                                   >  1:7397474/1‑93 (MQ=255)
                                                       aTACTTTGCGACCACGGACTTTTCAGTTGGCGGCGAAGGGGGTTAAGGGGAATGAGTTT‑AGCCACCGTGTTCGGCCCAGTGATTAAAATAGCg                                  >  2:1841975/1‑93 (MQ=255)
                                                        tACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAAAAGCGt                                 >  1:9032296/1‑93 (MQ=255)
                                                        tACTTTGCGACCACGGACTTATCAGTTCGCGGAGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGt                                 >  1:5708171/1‑93 (MQ=255)
                                                         aCTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTg                                <  2:1262290/93‑1 (MQ=255)
                                                          cTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGa                               >  1:2282600/1‑93 (MQ=255)
                                                           tttGCTACCACGGGCTTATCAGTTTGCCGCGGACAGCGGTCAGGCGAATGCGTTTCAACCA‑CGCGTTCGTGCCAATACCTAAAATACCGTGaa                              >  2:4830883/1‑93 (MQ=255)
                                                             tGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAAc                            <  2:3353885/93‑1 (MQ=255)
                                                             tGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAAc                            <  1:9691911/93‑1 (MQ=255)
                                                                gACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTCCGGGCCAATGCCTAAAATAGTGTGAAAcgcg                         >  2:8984668/1‑93 (MQ=255)
                                                                 aCCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGt                        >  1:9811125/1‑93 (MQ=255)
                                                                 aCCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGt                        >  2:5881027/1‑93 (MQ=255)
                                                                   cACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑TGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGttt                      <  2:6596393/93‑1 (MQ=255)
                                                                   cACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGttt                      <  2:7330493/93‑1 (MQ=255)
                                                                   cACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGttt                      >  1:1847785/1‑93 (MQ=255)
                                                                   cACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGttt                      <  1:6159197/93‑1 (MQ=255)
                                                                    aCGGACTTATCAGTTCGCAGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGtttt                     <  2:2119659/93‑1 (MQ=255)
                                                                      ggACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCCATGCCTAAAAGAGCGTGAAACGCGTTTTGt                   >  2:3025642/1‑93 (MQ=255)
                                                                      ggACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGt                   <  2:2864818/93‑1 (MQ=255)
                                                                       gACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTa                  >  1:8896220/1‑93 (MQ=255)
                                                                        aCTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAg                 <  1:10018795/93‑1 (MQ=255)
                                                                        aCTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAg                 <  1:4621083/93‑1 (MQ=255)
                                                                        aCTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCC‑ATGGCTAAAATAGCGTCAAACGGCGTTTGTaac                >  2:5811248/1‑91 (MQ=255)
                                                                          ttATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCg               >  2:8192284/1‑93 (MQ=255)
                                                                          ttATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCg               >  1:1172641/1‑93 (MQ=255)
                                                                          ttATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCg               >  1:6066259/1‑93 (MQ=255)
                                                                           tATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGt              <  2:1671367/93‑1 (MQ=255)
                                                                           tATCAGTTCGCGGCGCACGGGGTTCAGGCGAATGCGTTTCAGCAA‑CCCGTTCGGCCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCgg              >  2:7834800/1‑92 (MQ=255)
                                                                                gTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTgcggc         >  1:5872673/1‑93 (MQ=255)
                                                                                 ttCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTgcggcg        >  1:5794061/1‑93 (MQ=255)
                                                                                 ttCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTgcggcg        >  1:3337654/1‑93 (MQ=255)
                                                                                 ttCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTgcggcg        >  1:7250436/1‑93 (MQ=255)
                                                                                    gcggcgCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCt     >  2:8327426/1‑93 (MQ=255)
                                                                                    gcggcgCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCt     >  1:518192/1‑93 (MQ=255)
                                                                                      ggcgCACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGa   >  1:3965843/1‑93 (MQ=255)
                                                                                       gcgcACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGAc  <  1:3652682/93‑1 (MQ=255)
                                                                                          |                                                                                          
GGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCA‑CGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGAC  >  NC_000913/2552222‑2552401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACATGC  >  NC_000913/2552219‑2552405
                                                                                             |                                                                                             
CGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGG                                                                                               >  SRR4302540.1250569/1‑94 (MQ=60)
CGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGC                                                                                               >  SRR4302540.993548/1‑94 (MQ=60)
 GTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATCCTTTGCGACCACGGACTTATCAGTTCGCGGCGCA                                                                                              <  SRR4302540.3780274/94‑1 (MQ=60)
 GTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCA                                                                                              <  SRR4302540.1739965/94‑1 (MQ=60)
 GTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCA                                                                                              >  SRR4302540.8145516/1‑94 (MQ=60)
   GGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACG                                                                                            <  SRR4302540.5938482/94‑1 (MQ=60)
   GGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACG                                                                                            <  SRR4302540.4635544/94‑1 (MQ=60)
    GCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGG                                                                                           >  SRR4302540.3316016/1‑94 (MQ=60)
      AAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCG                                                                                         >  SRR4302540.7470980/1‑94 (MQ=60)
      AAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCG                                                                                         >  SRR4302540.1508820/1‑94 (MQ=60)
      AAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTACGCGGCGCACGGCG                                                                                         >  SRR4302540.6852211/1‑94 (MQ=60)
       AATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCATTTCGCGGCGCACGGCGT                                                                                        >  SRR4302540.8471585/1‑94 (MQ=60)
       AATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGT                                                                                        >  SRR4302540.193742/1‑94 (MQ=60)
        ATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTT                                                                                       >  SRR4302540.7172701/1‑94 (MQ=60)
        ATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTT                                                                                       <  SRR4302540.526601/94‑1 (MQ=60)
         TTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTC                                                                                      <  SRR4302540.4642395/94‑1 (MQ=60)
          TGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCA                                                                                     >  SRR4302540.2197197/1‑94 (MQ=60)
            CCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGG                                                                                   >  SRR4302540.9171097/1‑94 (MQ=60)
              CCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCG                                                                                 >  SRR4302540.6822861/1‑94 (MQ=60)
              CCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCG                                                                                 >  SRR4302540.464233/1‑94 (MQ=60)
              CCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCG                                                                                 >  SRR4302540.232271/1‑94 (MQ=60)
                AGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGAACGGCGTTCAGGCGAG                                                                               >  SRR4302540.3551732/1‑94 (MQ=60)
                 GATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAAT                                                                              >  SRR4302540.8472484/1‑94 (MQ=60)
                 GATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAAT                                                                              >  SRR4302540.762561/1‑94 (MQ=60)
                  ATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATG                                                                             >  SRR4302540.5918287/1‑94 (MQ=60)
                   TGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGC                                                                            <  SRR4302540.10090414/94‑1 (MQ=60)
                    GCCAGAAACCCCACTGGATGGCGGCGTCTTTTAACGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCG                                                                           <  SRR4302540.7061317/94‑1 (MQ=60)
                      CAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCCGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTT                                                                         >  SRR4302540.9818845/1‑94 (MQ=60)
                      CAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTT                                                                         >  SRR4302540.3097175/1‑94 (MQ=60)
                       AGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTT                                                                        <  SRR4302540.2098471/94‑1 (MQ=60)
                         AATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCA                                                                      >  SRR4302540.1769647/1‑94 (MQ=60)
                         AATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCA                                                                      <  SRR4302540.9339427/94‑1 (MQ=60)
                          ATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAG                                                                     <  SRR4302540.3440687/94‑1 (MQ=60)
                          ATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAG                                                                     >  SRR4302540.4500034/1‑94 (MQ=60)
                            CCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCC                                                                   <  SRR4302540.5412208/94‑1 (MQ=60)
                             CCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCC                                                                  >  SRR4302540.6693531/1‑94 (MQ=60)
                             CCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA                                                                  <  SRR4302540.5864400/94‑1 (MQ=60)
                             CCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA                                                                  >  SRR4302540.50177/1‑94 (MQ=60)
                             CCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCA                                                                  <  SRR4302540.6031111/94‑1 (MQ=60)
                              CCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCAC                                                                 >  SRR4302540.1119086/1‑94 (MQ=60)
                              CCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCAC                                                                 <  SRR4302540.2239764/94‑1 (MQ=60)
                              CCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCAC                                                                 <  SRR4302540.5281943/94‑1 (MQ=60)
                               CACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACG                                                                >  SRR4302540.1373187/1‑94 (MQ=60)
                               CACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACG                                                                >  SRR4302540.1373186/1‑94 (MQ=60)
                               CACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGAGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACG                                                                >  SRR4302540.6066948/1‑94 (MQ=60)
                               CACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTGATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACG                                                                <  SRR4302540.4179545/94‑1 (MQ=60)
                                ACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGC                                                               >  SRR4302540.843841/1‑94 (MQ=60)
                                ACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGC                                                               >  SRR4302540.843840/1‑94 (MQ=60)
                                 CTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCG                                                              >  SRR4302540.983061/1‑94 (MQ=60)
                                    CATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTC                                                           <  SRR4302540.6659816/94‑1 (MQ=60)
                                    CATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTC                                                           >  SRR4302540.8718122/1‑94 (MQ=60)
                                    CATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTC                                                           <  SRR4302540.5099164/94‑1 (MQ=60)
                                    CATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTC                                                           <  SRR4302540.3060019/94‑1 (MQ=60)
                                    CATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTC                                                           <  SRR4302540.6883697/94‑1 (MQ=60)
                                     CTGGCGGCGTCTTTTACCGTCATACTTTGCAACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCG                                                          <  SRR4302540.7004353/94‑1 (MQ=60)
                                     ATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCG                                                          >  SRR4302540.2670616/1‑94 (MQ=60)
                                     ATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCG                                                          <  SRR4302540.5782031/94‑1 (MQ=60)
                                      TGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGG                                                         <  SRR4302540.1430935/94‑1 (MQ=60)
                                       GGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGG                                                        <  SRR4302540.2782554/94‑1 (MQ=60)
                                         CGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCTGCGCACGGCGTTCAGGTGAATGCGTATCTTCCACGCGTTCGGGCC                                                      >  SRR4302540.9042026/1‑94 (MQ=60)
                                         CGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCC                                                      <  SRR4302540.8422044/94‑1 (MQ=60)
                                          GGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCA                                                     >  SRR4302540.5530531/1‑94 (MQ=60)
                                            CGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAAT                                                   <  SRR4302540.7287742/94‑1 (MQ=60)
                                            CGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAAT                                                   <  SRR4302540.1720050/94‑1 (MQ=60)
                                            CGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAAT                                                   >  SRR4302540.597691/1‑94 (MQ=60)
                                            CGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAAT                                                   <  SRR4302540.6021328/94‑1 (MQ=60)
                                            CGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAAT                                                   >  SRR4302540.9553301/1‑94 (MQ=60)
                                             GTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATG                                                  >  SRR4302540.2912168/1‑94 (MQ=60)
                                             GTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATG                                                  >  SRR4302540.5460765/1‑94 (MQ=60)
                                             GTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATG                                                  >  SRR4302540.2353169/1‑94 (MQ=60)
                                             CTCTTTTCCCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCATGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATG                                                  <  SRR4302540.9414808/94‑1 (MQ=60)
                                              TCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGC                                                 <  SRR4302540.9540421/94‑1 (MQ=60)
                                               CTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCC                                                >  SRR4302540.7594051/1‑94 (MQ=60)
                                                TTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCT                                               >  SRR4302540.2602601/1‑94 (MQ=60)
                                                TTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCT                                               <  SRR4302540.1736125/94‑1 (MQ=60)
                                                  TTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAA                                             <  SRR4302540.9286398/94‑1 (MQ=60)
                                                   TACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAA                                            <  SRR4302540.1422994/94‑1 (MQ=60)
                                                    ACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAA                                           <  SRR4302540.447983/94‑1 (MQ=60)
                                                      CGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATA                                         <  SRR4302540.5245978/94‑1 (MQ=60)
                                                      CGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATA                                         <  SRR4302540.4881382/94‑1 (MQ=60)
                                                       GTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAG                                        >  SRR4302540.6653991/1‑94 (MQ=60)
                                                       GTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAG                                        >  SRR4302540.5554907/1‑94 (MQ=60)
                                                       GTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAG                                        >  SRR4302540.4808688/1‑94 (MQ=60)
                                                       GTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAG                                        >  SRR4302540.7712678/1‑94 (MQ=60)
                                                        TCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGC                                       >  SRR4302540.6580889/1‑94 (MQ=60)
                                                        TCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGC                                       >  SRR4302540.7479893/1‑94 (MQ=60)
                                                         CATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCG                                      <  SRR4302540.6338181/94‑1 (MQ=60)
                                                          ATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAAAAGCGT                                     >  SRR4302540.9140688/1‑94 (MQ=60)
                                                          ATACTTTGCGACCACGGACTTATCAGTTCGCGGAGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGT                                     >  SRR4302540.5771012/1‑94 (MQ=60)
                                                           TACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTG                                    >  SRR4302540.7347761/1‑94 (MQ=60)
                                                            ACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGA                                   >  SRR4302540.4750915/1‑94 (MQ=60)
                                                            ACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGA                                   >  SRR4302540.2307128/1‑94 (MQ=60)
                                                             CTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAA                                  >  SRR4302540.9868478/1‑94 (MQ=60)
                                                              TTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAA                                 >  SRR4302540.2972030/1‑94 (MQ=60)
                                                              TTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAA                                 <  SRR4302540.9645683/94‑1 (MQ=60)
                                                                TGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACG                               <  SRR4302540.9805897/94‑1 (MQ=60)
                                                                  CGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCG                             >  SRR4302540.3134285/1‑94 (MQ=60)
                                                                  CGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCG                             >  SRR4302540.2746619/1‑94 (MQ=60)
                                                                   GACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGT                            >  SRR4302540.9925947/1‑94 (MQ=60)
                                                                     CCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTT                          <  SRR4302540.5503472/94‑1 (MQ=60)
                                                                     CCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTT                          >  SRR4302540.1868049/1‑94 (MQ=60)
                                                                      CACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTT                         <  SRR4302540.6227822/94‑1 (MQ=60)
                                                                      CACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTT                         <  SRR4302540.3675470/94‑1 (MQ=60)
                                                                       ACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTG                        <  SRR4302540.9306593/94‑1 (MQ=60)
                                                                        CGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGT                       <  SRR4302540.3079177/94‑1 (MQ=60)
                                                                         GGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTA                      >  SRR4302540.9309712/1‑94 (MQ=60)
                                                                         GGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTA                      >  SRR4302540.8999199/1‑94 (MQ=60)
                                                                         GGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAAGGCCTAAAATAGCGTGAAACGCGTTTTGTA                      >  SRR4302540.3507258/1‑94 (MQ=60)
                                                                          GACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAG                     <  SRR4302540.1631482/94‑1 (MQ=60)
                                                                          GACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAG                     <  SRR4302540.4185755/94‑1 (MQ=60)
                                                                          GACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAG                     <  SRR4302540.1987548/94‑1 (MQ=60)
                                                                           GCTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGC                    <  SRR4302540.10072326/94‑1 (MQ=60)
                                                                           ACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGC                    <  SRR4302540.7011255/94‑1 (MQ=60)
                                                                           ACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGC                    <  SRR4302540.4670618/94‑1 (MQ=60)
                                                                           ACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGC                    <  SRR4302540.10135406/94‑1 (MQ=60)
                                                                            CTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCACTGCCTAAAATAGCGTGAAACGCGTTTTGTAGCG                   >  SRR4302540.9808955/1‑94 (MQ=60)
                                                                            CTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCG                   >  SRR4302540.6133707/1‑94 (MQ=60)
                                                                            CTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCG                   >  SRR4302540.6133706/1‑94 (MQ=60)
                                                                            CTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCG                   >  SRR4302540.5064510/1‑94 (MQ=60)
                                                                            CTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCG                   <  SRR4302540.5681173/94‑1 (MQ=60)
                                                                            CTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCG                   >  SRR4302540.1184774/1‑94 (MQ=60)
                                                                             TTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGT                  <  SRR4302540.2549028/94‑1 (MQ=60)
                                                                             TTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGT                  >  SRR4302540.2734685/1‑94 (MQ=60)
                                                                              TATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTG                 <  SRR4302540.9067164/94‑1 (MQ=60)
                                                                               ATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGC                >  SRR4302540.7844198/1‑94 (MQ=60)
                                                                                TCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCG               <  SRR4302540.3934424/94‑1 (MQ=60)
                                                                                  AGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGC             >  SRR4302540.4512636/1‑94 (MQ=60)
                                                                                  AGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGC             >  SRR4302540.5937585/1‑94 (MQ=60)
                                                                                   GTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCG            >  SRR4302540.7331339/1‑94 (MQ=60)
                                                                                   GTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCG            >  SRR4302540.3374305/1‑94 (MQ=60)
                                                                                   GTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCG            >  SRR4302540.3627099/1‑94 (MQ=60)
                                                                                   GTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCG            >  SRR4302540.9669062/1‑94 (MQ=60)
                                                                                   GTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCG            <  SRR4302540.9519352/94‑1 (MQ=60)
                                                                                   GTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCG            <  SRR4302540.4829265/94‑1 (MQ=60)
                                                                                   GTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCG            >  SRR4302540.5858085/1‑94 (MQ=60)
                                                                                      CGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCT         >  SRR4302540.523748/1‑94 (MQ=60)
                                                                                       GCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTG        >  SRR4302540.1463215/1‑94 (MQ=60)
                                                                                        CGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGA       >  SRR4302540.4008597/1‑94 (MQ=60)
                                                                                        CGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGA       >  SRR4302540.5560999/1‑94 (MQ=60)
                                                                                          GCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACA     <  SRR4302540.3692689/94‑1 (MQ=60)
                                                                                           CGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGTTGACAT    <  SRR4302540.4930186/94‑1 (MQ=60)
                                                                                           CGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACAT    <  SRR4302540.5355266/94‑1 (MQ=60)
                                                                                           CGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACAT    >  SRR4302540.8975750/1‑94 (MQ=60)
                                                                                             CACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACATGC  >  SRR4302540.5103347/1‑94 (MQ=60)
                                                                                             CACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACATGC  >  SRR4302540.5103348/1‑94 (MQ=60)
                                                                                             CACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACATGC  <  SRR4302540.3746971/94‑1 (MQ=60)
                                                                                             CACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACATGC  <  SRR4302540.7099365/94‑1 (MQ=60)
                                                                                             CACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACATGC  <  SRR4302540.568806/94‑1 (MQ=60)
                                                                                             |                                                                                             
CGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACATGC  >  NC_000913/2552219‑2552405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑