Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,552,312 T→C V297V (GTA→GTG)  yfeG ← putative ARAC‑type regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,552,3120TC100.0% 758.5 / NA 238V297V (GTA→GTG) yfeGputative ARAC‑type regulatory protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (105/133);  total (105/133)

Only 100 of 242 total aligned reads displayed.

GGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGAC  >  NC_000913/2552222‑2552401
                                                                                          |                                                                                         
    aaTTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCg                                                                                     >  1:1695926/1‑93 (MQ=255)
     aTTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGt                                                                                    >  2:5712471/1‑93 (MQ=255)
     aTTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGt                                                                                    <  1:3165003/93‑1 (MQ=255)
      ttGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGtt                                                                                   >  2:2520039/1‑93 (MQ=255)
      ttGCCCCAGACGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTTCGACCACGGACTTATCAGTTCGCGGCGCACGGCGtt                                                                                   >  2:3251547/1‑93 (MQ=255)
        gCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCa                                                                                 <  2:5171730/93‑1 (MQ=255)
        gCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCa                                                                                 <  2:1770446/93‑1 (MQ=255)
        gCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCa                                                                                 >  2:4733758/1‑93 (MQ=255)
         ccccAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAg                                                                                <  2:2396906/93‑1 (MQ=255)
          cccAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCATGGCGTTCAgg                                                                               <  2:1314338/93‑1 (MQ=255)
          cccAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAgg                                                                               <  1:541129/93‑1 (MQ=255)
          cccAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAgg                                                                               >  2:4563166/1‑93 (MQ=255)
          cccAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAgg                                                                               <  1:636428/93‑1 (MQ=255)
           ccAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGc                                                                              <  1:3599867/93‑1 (MQ=255)
            cAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCg                                                                             >  2:673314/1‑93 (MQ=255)
            cAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCg                                                                             <  1:6218637/93‑1 (MQ=255)
             aGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGCCTTATACGTTCGCGGCGCCAGCCGCTCAGGCGa                                                                            >  1:330528/1‑93 (MQ=255)
             aGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGa                                                                            <  2:5395517/93‑1 (MQ=255)
              gATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGaa                                                                           <  1:5671868/93‑1 (MQ=255)
              gATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGaa                                                                           <  1:695893/93‑1 (MQ=255)
              gATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGaa                                                                           >  1:4331209/1‑93 (MQ=255)
               aTGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAAt                                                                          <  1:4754111/93‑1 (MQ=255)
                tGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATg                                                                         <  1:2735618/93‑1 (MQ=255)
                tGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATg                                                                         <  2:4815192/93‑1 (MQ=255)
                tGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATg                                                                         <  1:1788314/93‑1 (MQ=255)
                tGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATg                                                                         <  2:3626298/93‑1 (MQ=255)
                 gCCAGCATCCCCACTGCATGGCCGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGc                                                                        <  2:5112649/93‑1 (MQ=255)
                 gCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGc                                                                        <  1:646928/93‑1 (MQ=255)
                   cAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGt                                                                      <  1:2466617/93‑1 (MQ=255)
                   cAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGt                                                                      >  1:5812985/1‑93 (MQ=255)
                    aGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGtt                                                                     >  1:240885/1‑93 (MQ=255)
                      aaTCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTc                                                                   >  1:2544371/1‑93 (MQ=255)
                      aaTCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTc                                                                   <  1:52021/93‑1 (MQ=255)
                      aaTCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTc                                                                   <  1:2339833/93‑1 (MQ=255)
                       aTCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCa                                                                  >  2:3538875/1‑93 (MQ=255)
                         ccccACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGc                                                                <  1:2954337/93‑1 (MQ=255)
                          cccACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAgac                                                               <  1:413614/93‑3 (MQ=255)
                          cccACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGcc                                                               <  2:1005283/93‑1 (MQ=255)
                          cccACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGcc                                                               >  1:628799/1‑93 (MQ=255)
                           ccACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCa                                                              <  1:502472/93‑1 (MQ=255)
                            cACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCAc                                                             >  2:1215002/1‑93 (MQ=255)
                               tGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGTGTTCAGGCGAATGCGTTTCAGCCAcgcg                                                          >  2:1572660/1‑93 (MQ=255)
                                gCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGt                                                         >  2:1808245/1‑93 (MQ=255)
                                gCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGt                                                         <  1:4285245/93‑1 (MQ=255)
                                 cATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGtt                                                        <  1:167863/93‑1 (MQ=255)
                                 cATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGtt                                                        <  2:6065845/93‑1 (MQ=255)
                                      cggcgTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGc                                                   >  1:5082403/1‑93 (MQ=255)
                                      cggcgTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGc                                                   <  2:5340302/93‑1 (MQ=255)
                                       ggcgTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGcc                                                  >  2:3933985/1‑93 (MQ=255)
                                        gcgTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGTCa                                                 >  2:2676506/1‑93 (MQ=255)
                                        gcgTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCa                                                 >  2:451147/1‑93 (MQ=255)
                                         cgTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCaa                                                <  1:3418387/93‑1 (MQ=255)
                                         cgTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCaa                                                >  1:4635022/1‑93 (MQ=255)
                                         cgTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCaa                                                <  1:6269478/93‑1 (MQ=255)
                                            cTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGc                                             <  2:732305/93‑1 (MQ=255)
                                                 aCCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTaaa                                        >  1:1235172/1‑93 (MQ=255)
                                                   cGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAAt                                      <  1:3864423/93‑1 (MQ=255)
                                                   cGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGGTCAGGCGAATGCGTTTTAGCCACGCGTTCGGGGCAATGCCTACAAt                                      >  2:2243862/1‑93 (MQ=255)
                                                    gTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATa                                     <  2:5550815/93‑1 (MQ=255)
                                                     tCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAg                                    <  2:5736359/93‑1 (MQ=255)
                                                      cATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGc                                   <  2:4113786/93‑1 (MQ=255)
                                                      cATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGc                                   >  1:6019025/1‑93 (MQ=255)
                                                      cATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGc                                   <  2:5629658/93‑1 (MQ=255)
                                                       aTACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCg                                  <  2:4215488/93‑1 (MQ=255)
                                                       aTACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCg                                  >  1:3943045/1‑93 (MQ=255)
                                                        ttcTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGt                                 <  1:4518717/91‑1 (MQ=255)
                                                         aCTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTg                                <  1:883555/93‑1 (MQ=255)
                                                           tttGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGaa                              >  1:1657574/1‑93 (MQ=255)
                                                           tttGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGaa                              >  1:3721494/1‑93 (MQ=255)
                                                            ttGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGaaa                             <  2:2902295/93‑1 (MQ=255)
                                                            ttGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGaaa                             <  1:2195166/93‑1 (MQ=255)
                                                            ttGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGaaa                             <  2:5831244/93‑1 (MQ=255)
                                                               cGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAAcgc                          <  2:5088264/93‑1 (MQ=255)
                                                               cGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAAcgc                          <  1:1031809/93‑1 (MQ=255)
                                                                 aCCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGt                        >  1:2166939/1‑93 (MQ=255)
                                                                  ccACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGtt                       <  2:4056833/93‑1 (MQ=255)
                                                                  ccACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGtt                       <  1:4629173/93‑1 (MQ=255)
                                                                  ccACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGtt                       <  2:1895786/93‑1 (MQ=255)
                                                                   cACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGttt                      >  1:4000417/1‑93 (MQ=255)
                                                                    aCGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGtttt                     <  2:3070603/93‑1 (MQ=255)
                                                                    aCGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGtttt                     <  2:4442122/93‑1 (MQ=255)
                                                                     cGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTg                    <  1:1169329/93‑1 (MQ=255)
                                                                      ggACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGt                   >  2:3836909/1‑93 (MQ=255)
                                                                      ggACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGt                   <  1:2096251/93‑1 (MQ=255)
                                                                      ggACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGt                   <  2:2279129/93‑1 (MQ=255)
                                                                       gACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTa                  <  1:1360418/93‑1 (MQ=255)
                                                                        aCTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAg                 <  2:4271568/93‑1 (MQ=255)
                                                                          ttATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCg               >  1:2702458/1‑93 (MQ=255)
                                                                          ttATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCg               >  2:1989372/1‑93 (MQ=255)
                                                                           tATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGt              <  2:6112882/93‑1 (MQ=255)
                                                                             tCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTgc            >  2:3317043/1‑93 (MQ=255)
                                                                             tCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTgc            <  1:1450366/93‑1 (MQ=255)
                                                                              cAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTgcg           >  2:2581123/1‑93 (MQ=255)
                                                                              cAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTgcg           <  1:2245107/93‑1 (MQ=255)
                                                                                gTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTgcggc         <  1:2835594/93‑1 (MQ=255)
                                                                                gTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTgcggc         <  1:58838/93‑1 (MQ=255)
                                                                                gTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTgcggc         <  1:1725374/93‑1 (MQ=255)
                                                                                   cgcgGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCggcggc      >  2:3948922/1‑93 (MQ=255)
                                                                                    gcggcgCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCt     <  1:758598/93‑1 (MQ=255)
                                                                                       gcgcACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGAc  <  1:4923231/93‑1 (MQ=255)
                                                                                          |                                                                                         
GGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGAC  >  NC_000913/2552222‑2552401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACATGC  >  NC_000913/2552219‑2552405
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CGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGC                                                                                               >  SRR4302533.4883947/1‑94 (MQ=60)
 GTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCA                                                                                              <  SRR4302533.3319853/94‑1 (MQ=60)
 GTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCA                                                                                              >  SRR4302533.2658419/1‑94 (MQ=60)
 GTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCA                                                                                              >  SRR4302533.2387003/1‑94 (MQ=60)
   GGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACG                                                                                            <  SRR4302533.2439020/94‑1 (MQ=60)
    GCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGG                                                                                           <  SRR4302533.4321998/94‑1 (MQ=60)
     CAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGC                                                                                          >  SRR4302533.3903423/1‑94 (MQ=60)
      AAGTTGCCCGAGATGCCAGAATCCCCACTGCATGGCGTCTTCTTTTACCGTCATCCTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCG                                                                                         <  SRR4302533.2191801/94‑1 (MQ=60)
      AAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCG                                                                                         >  SRR4302533.6347683/1‑94 (MQ=60)
      AAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCG                                                                                         >  SRR4302533.1706198/1‑94 (MQ=60)
        ATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTT                                                                                       <  SRR4302533.3184480/94‑1 (MQ=60)
           GCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAG                                                                                    >  SRR4302533.1555497/1‑94 (MQ=60)
            CCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGG                                                                                   <  SRR4302533.2489789/94‑1 (MQ=60)
             CCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGC                                                                                  <  SRR4302533.544349/94‑1 (MQ=60)
             CCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGC                                                                                  <  SRR4302533.640175/94‑1 (MQ=60)
             CCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGC                                                                                  >  SRR4302533.3291268/1‑94 (MQ=60)
              CCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCG                                                                                 <  SRR4302533.2925954/94‑1 (MQ=60)
              CCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCG                                                                                 <  SRR4302533.3358691/94‑1 (MQ=60)
              CCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCG                                                                                 >  SRR4302533.4358222/1‑94 (MQ=60)
              CCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCG                                                                                 <  SRR4302533.3622014/94‑1 (MQ=60)
               CAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGA                                                                                <  SRR4302533.6255922/94‑1 (MQ=60)
                AGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAA                                                                               >  SRR4302533.4357305/1‑94 (MQ=60)
                 GATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAAT                                                                              <  SRR4302533.5706121/94‑1 (MQ=60)
                 GATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAAT                                                                              <  SRR4302533.700007/94‑1 (MQ=60)
                  ATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATG                                                                             <  SRR4302533.4782929/94‑1 (MQ=60)
                   TGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGC                                                                            <  SRR4302533.1799186/94‑1 (MQ=60)
                   TGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGC                                                                            <  SRR4302533.2752400/94‑1 (MQ=60)
                    GCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCG                                                                           <  SRR4302533.650739/94‑1 (MQ=60)
                    GCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCG                                                                           <  SRR4302533.650740/94‑1 (MQ=60)
                     CCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGT                                                                          >  SRR4302533.5848093/1‑94 (MQ=60)
                      CAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTT                                                                         <  SRR4302533.2481833/94‑1 (MQ=60)
                      CAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTT                                                                         >  SRR4302533.242381/1‑94 (MQ=60)
                        GAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTC                                                                       >  SRR4302533.2560029/1‑94 (MQ=60)
                         AATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCA                                                                      <  SRR4302533.2354322/94‑1 (MQ=60)
                         AATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCA                                                                      <  SRR4302533.52372/94‑1 (MQ=60)
                         AATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCA                                                                      <  SRR4302533.138465/94‑1 (MQ=60)
                          ATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAG                                                                     >  SRR4302533.2178398/1‑94 (MQ=60)
                            CCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCC                                                                   >  SRR4302533.632499/1‑94 (MQ=60)
                            CCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCC                                                                   <  SRR4302533.2972405/94‑1 (MQ=60)
                            CCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCC                                                                   >  SRR4302533.1854444/1‑94 (MQ=60)
                             CCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGACA                                                                  <  SRR4302533.416096/94‑1 (MQ=60)
                              CCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCAC                                                                 <  SRR4302533.505458/94‑1 (MQ=60)
                                  TGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGT                                                             >  SRR4302533.460763/1‑94 (MQ=60)
                                   GCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTT                                                            <  SRR4302533.4310993/94‑1 (MQ=60)
                                   GCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTT                                                            <  SRR4302533.2790692/94‑1 (MQ=60)
                                    CATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTC                                                           <  SRR4302533.168924/94‑1 (MQ=60)
                                     ATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCG                                                          <  SRR4302533.506971/94‑1 (MQ=60)
                                      TGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGG                                                         >  SRR4302533.1672123/1‑94 (MQ=60)
                                      TGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGG                                                         <  SRR4302533.765330/94‑1 (MQ=60)
                                        GCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGC                                                       >  SRR4302533.5113122/1‑94 (MQ=60)
                                         CGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCC                                                      <  SRR4302533.738514/94‑1 (MQ=60)
                                          GGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCA                                                     >  SRR4302533.4164228/1‑94 (MQ=60)
                                           GCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAA                                                    >  SRR4302533.4663108/1‑94 (MQ=60)
                                           GCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAA                                                    >  SRR4302533.1308684/1‑94 (MQ=60)
                                            CGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAAT                                                   <  SRR4302533.3439510/94‑1 (MQ=60)
                                            CGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAAT                                                   >  SRR4302533.1097076/1‑94 (MQ=60)
                                            CGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAAT                                                   <  SRR4302533.3140111/94‑1 (MQ=60)
                                            CGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAAT                                                   <  SRR4302533.6306938/94‑1 (MQ=60)
                                             GTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATG                                                  <  SRR4302533.2770982/94‑1 (MQ=60)
                                             GTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGATCGGGCCAATG                                                  >  SRR4302533.918512/1‑94 (MQ=60)
                                              TCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGC                                                 >  SRR4302533.4698454/1‑94 (MQ=60)
                                               CTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCC                                                <  SRR4302533.5403135/94‑1 (MQ=60)
                                               CTTTTACCGACATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCC                                                <  SRR4302533.4855943/94‑1 (MQ=60)
                                                  TTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAA                                             >  SRR4302533.2389423/1‑94 (MQ=60)
                                                   TACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAA                                            >  SRR4302533.1242555/1‑94 (MQ=60)
                                                     CCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAAT                                          <  SRR4302533.5703370/94‑1 (MQ=60)
                                                     CCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAAT                                          >  SRR4302533.222648/1‑94 (MQ=60)
                                                      CGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATA                                         >  SRR4302533.6310317/1‑94 (MQ=60)
                                                      CGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATA                                         <  SRR4302533.5019882/94‑1 (MQ=60)
                                                      CGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATA                                         <  SRR4302533.3888013/94‑1 (MQ=60)
                                                        TCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGC                                       >  SRR4302533.6055499/1‑94 (MQ=60)
                                                        TCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGC                                       <  SRR4302533.3801720/94‑1 (MQ=60)
                                                         CATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCG                                      >  SRR4302533.3967101/1‑94 (MQ=60)
                                                         CATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCG                                      <  SRR4302533.837248/94‑1 (MQ=60)
                                                         CATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCG                                      <  SRR4302533.4687201/94‑1 (MQ=60)
                                                         CATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCG                                      >  SRR4302533.1340677/1‑94 (MQ=60)
                                                          ATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGT                                     <  SRR4302533.2553078/94‑1 (MQ=60)
                                                          ATACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGT                                     >  SRR4302533.3459678/1‑94 (MQ=60)
                                                          ATACTTTGCGACCACGGACTTATCAGTTCGCCGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGATCGGGCCAATGCCTAAAATAGCGC                                     >  SRR4302533.4029786/1‑94 (MQ=60)
                                                           TTCTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTG                                    <  SRR4302533.4546083/94‑1 (MQ=60)
                                                           TACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTG                                    <  SRR4302533.2694946/94‑1 (MQ=60)
                                                            ACTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGA                                   <  SRR4302533.888743/94‑1 (MQ=60)
                                                             CTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAA                                  >  SRR4302533.3744333/1‑94 (MQ=60)
                                                             CTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAA                                  >  SRR4302533.1667605/1‑94 (MQ=60)
                                                             CTTTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAA                                  >  SRR4302533.1210606/1‑94 (MQ=60)
                                                               TTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAAC                                <  SRR4302533.3167236/94‑1 (MQ=60)
                                                               TTGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAAC                                <  SRR4302533.2208691/94‑1 (MQ=60)
                                                                TGCGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACG                               <  SRR4302533.5171959/94‑1 (MQ=60)
                                                                  CGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCG                             >  SRR4302533.3679547/1‑94 (MQ=60)
                                                                  CGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCG                             <  SRR4302533.1499041/94‑1 (MQ=60)
                                                                  CGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCG                             <  SRR4302533.1037877/94‑1 (MQ=60)
                                                                  CGACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCG                             >  SRR4302533.229101/1‑94 (MQ=60)
                                                                   GACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGT                            <  SRR4302533.6312619/94‑1 (MQ=60)
                                                                   GACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGT                            >  SRR4302533.5828151/1‑94 (MQ=60)
                                                                   GACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGT                            >  SRR4302533.2180266/1‑94 (MQ=60)
                                                                   GACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGT                            >  SRR4302533.2192462/1‑94 (MQ=60)
                                                                   GACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGT                            >  SRR4302533.3802285/1‑94 (MQ=60)
                                                                    ACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTT                           >  SRR4302533.694688/1‑94 (MQ=60)
                                                                    ACCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTT                           >  SRR4302533.644990/1‑94 (MQ=60)
                                                                     CCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTT                          >  SRR4302533.4024775/1‑94 (MQ=60)
                                                                     CCACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTT                          <  SRR4302533.4657230/94‑1 (MQ=60)
                                                                       CCGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTG                        <  SRR4302533.3977495/94‑1 (MQ=60)
                                                                       ACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTG                        >  SRR4302533.2743208/1‑94 (MQ=60)
                                                                       ACGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTG                        <  SRR4302533.5795739/94‑1 (MQ=60)
                                                                        CGGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGT                       <  SRR4302533.1176347/94‑1 (MQ=60)
                                                                         GGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTA                      >  SRR4302533.172105/1‑94 (MQ=60)
                                                                         GGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTA                      <  SRR4302533.2109157/94‑1 (MQ=60)
                                                                         GGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTA                      >  SRR4302533.6159967/1‑94 (MQ=60)
                                                                         GGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGAGTGAAACGCGTTTTGTA                      >  SRR4302533.1729187/1‑94 (MQ=60)
                                                                         GGACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCAGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTA                      >  SRR4302533.5918836/1‑94 (MQ=60)
                                                                          GACTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAG                     <  SRR4302533.1368591/94‑1 (MQ=60)
                                                                            CTTATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCG                   >  SRR4302533.2719035/1‑94 (MQ=60)
                                                                              TATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTG                 <  SRR4302533.401599/94‑1 (MQ=60)
                                                                              TATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTG                 >  SRR4302533.4059235/1‑94 (MQ=60)
                                                                               ATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGC                >  SRR4302533.1761750/1‑94 (MQ=60)
                                                                               ATCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGC                >  SRR4302533.3383999/1‑94 (MQ=60)
                                                                                TCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCG               <  SRR4302533.6050330/94‑1 (MQ=60)
                                                                                TCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCG               <  SRR4302533.6281040/94‑1 (MQ=60)
                                                                                TCAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCG               <  SRR4302533.1459118/94‑1 (MQ=60)
                                                                                 CAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGG              <  SRR4302533.2258980/94‑1 (MQ=60)
                                                                                 CAGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGG              >  SRR4302533.4195853/1‑94 (MQ=60)
                                                                                  AGTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGC             >  SRR4302533.5413287/1‑94 (MQ=60)
                                                                                   GTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCG            <  SRR4302533.59225/94‑1 (MQ=60)
                                                                                   GTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCG            <  SRR4302533.2388104/94‑1 (MQ=60)
                                                                                   GTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCG            <  SRR4302533.2853042/94‑1 (MQ=60)
                                                                                   GTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCG            <  SRR4302533.1735844/94‑1 (MQ=60)
                                                                                   GTTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCG            <  SRR4302533.2053473/94‑1 (MQ=60)
                                                                                    TTCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGG           <  SRR4302533.4617988/94‑1 (MQ=60)
                                                                                     TCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGC          <  SRR4302533.1702774/94‑1 (MQ=60)
                                                                                     TCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGC          <  SRR4302533.6066060/94‑1 (MQ=60)
                                                                                     TCGCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGGTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGC          >  SRR4302533.4662635/1‑94 (MQ=60)
                                                                                       GCGGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTG        <  SRR4302533.763064/94‑1 (MQ=60)
                                                                                         GGCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGAC      <  SRR4302533.5104502/94‑1 (MQ=60)
                                                                                          GCGCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACA     <  SRR4302533.4953058/94‑1 (MQ=60)
                                                                                            GCACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACATG   >  SRR4302533.1312811/1‑94 (MQ=60)
                                                                                             CACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACATGC  >  SRR4302533.4678847/1‑94 (MQ=60)
                                                                                             CACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACATGC  >  SRR4302533.2827791/1‑94 (MQ=60)
                                                                                             CACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACATGC  >  SRR4302533.993842/1‑94 (MQ=60)
                                                                                             |                                                                                             
CGTGGCAAATTGCCCCAGATGCCAGAATCCCCACTGCATGGCGGCGTCTTTTACCGTCATACTTTGCGACCACGGACTTATCAGTTCGCGGCGTACGGCGTTCAGGCGAATGCGTTTCAGCCACGCGTTCGGGCCAATGCCTAAAATAGCGTGAAACGCGTTTTGTAGCGTGCGGCGGCTGACATGC  >  NC_000913/2552219‑2552405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑