Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,574,803	A→G	L532P (CTG→CCG)	b2463 ←	putative multimodular enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,574,803	0	A	G	99.2%	383.7 / ‑4.9	125	L532P (CTG→CCG)	b2463	putative multimodular enzyme
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (111/12); total (113/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.78e-01

Only 100 of 197 total aligned reads displayed.

TCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGA  >  NC_000913/2574713‑2574891

tCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGgc                                                                                        <  2:9693901/93‑1 (MQ=255)

tCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGgc                                                                                        >  2:3382632/1‑93 (MQ=255)

tCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGgc                                                                                        >  2:2738740/1‑93 (MQ=255)

  aTGATAATCCCCCACCGTACCGCAAATCATTTCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGgcgc                                                                                      <  2:2778568/93‑1 (MQ=255)

  aTGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGgcgc                                                                                      <  1:7130447/93‑1 (MQ=255)

   tGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGcc                                                                                     <  2:7303231/93‑1 (MQ=255)

   tGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGcc                                                                                     >  2:6580084/1‑93 (MQ=255)

   tGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGcc                                                                                     >  1:5011693/1‑93 (MQ=255)

    gATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGccc                                                                                    >  1:6304089/1‑93 (MQ=255)

      tAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGc                                                                                  >  2:1788450/1‑93 (MQ=255)

         tCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTgc                                                                               >  1:7035182/1‑93 (MQ=255)

         ccacccaccGTACCGCAAATCATTGCATCGGCTCCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTgc                                                                               <  1:9961303/92‑1 (MQ=255)

           acccaccGTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCtcgcc                                                                             >  1:7735850/1‑89 (MQ=255)

            cccaccGTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGcc                                                                            >  2:6631693/1‑93 (MQ=255)

            cccaccGTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGcc                                                                            >  1:566830/1‑93 (MQ=255)

            cccaccGTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGcc                                                                            >  1:6951022/1‑93 (MQ=255)

             ccaccGTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCt                                                                           >  2:3206627/1‑93 (MQ=255)

             ccaccGTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGCTACTGATCGGCGCCCGCTGCGCCt                                                                           >  2:759067/1‑93 (MQ=255)

              caccGTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTg                                                                          >  1:6566678/1‑93 (MQ=255)

               accGTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGt                                                                         >  1:9101568/1‑93 (MQ=255)

                ccGTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGtt                                                                        >  2:9069899/1‑93 (MQ=255)

                ccGTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGtt                                                                        >  1:5297029/1‑93 (MQ=255)

                ccGTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGtt                                                                        >  2:1509165/1‑93 (MQ=255)

                ccGTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGtt                                                                        >  1:7335006/1‑93 (MQ=255)

                  gTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTcc                                                                      >  2:4993242/1‑93 (MQ=255)

                       gCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGt                                                                 >  2:240577/1‑93 (MQ=255)

                        cAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTg                                                                >  1:4005291/1‑93 (MQ=255)

                         aaaTCCTTGCATCGGC‑TCCCAACAGTTAAACCTTGAGGACGCCCAACACTGTGGGTTAACAAATGGGCGCCCGTTGCGCCTGTTCCTGAGTGa                                                               <  2:5350905/93‑1 (MQ=255)

                         aaaTCATTGCATCGGCTCCCCCAC‑GCTGACCCATGATCGCGCCGATCACTGTGGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGa                                                               <  2:1011324/93‑1 (MQ=255)

                         aaaTCATTGCATCGGCTCCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGTTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGa                                                               <  2:1640199/93‑1 (MQ=255)

                          aaTCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGAc                                                              >  1:2653508/1‑93 (MQ=255)

                                 gCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGa                                                       >  1:4388139/1‑93 (MQ=255)

                                  cctCGGCTCCCCCAC‑GCTAACCCATGACCCCGCCGACCACTGTCGGTTTACTGACCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGAc                                                      <  2:8380606/91‑1 (MQ=255)

                                    tCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGc                                                    >  1:3657052/1‑93 (MQ=255)

                                    tCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGc                                                    >  1:1283498/1‑93 (MQ=255)

                                      ggCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCtt                                                  >  2:8429164/1‑93 (MQ=255)

                                      ggCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCtt                                                  >  2:9397296/1‑93 (MQ=255)

                                       gCTCCCCCAC‑GCGGACCCATGATCCCGCCGATCCCTGTCGGGTTACTGACCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTc                                                 <  2:3165616/93‑1 (MQ=255)

                                        ctccccccc‑GCTGAACCATGACCGCGCGGATCACTGTGGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCa                                                <  1:5084582/90‑1 (MQ=255)

                                        ctcccccAC‑GGTAAACCATGACCGCGCCTATCTCTGGCGGTTTACTGGGCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCa                                                <  1:3063164/90‑1 (MQ=255)

                                           ccccAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCAtca                                             >  1:2133735/1‑91 (MQ=255)

                                             ccAC‑GCTGACCCAGGATCGCGCCGATCGCTGTGGGGTTACGGAGCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATc                                           <  1:7663245/93‑1 (MQ=255)

                                             ccAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATc                                           >  1:4834146/1‑93 (MQ=255)

                                             ccAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATc                                           >  2:5968221/1‑93 (MQ=255)

                                              cAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCt                                          >  2:1908194/1‑93 (MQ=255)

                                               aC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTg                                         >  2:213146/1‑93 (MQ=255)

                                               aC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTg                                         >  1:5547414/1‑93 (MQ=255)

                                               aC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTg                                         >  1:9808112/1‑93 (MQ=255)

                                                   cTGAACCATGATCGCGCGGTTCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGaa                                      <  2:5511977/93‑1 (MQ=255)

                                                      acccATGATCGCGCCGATCACTGTGGGTTTACTGTTCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTa                                   <  2:6884837/91‑1 (MQ=255)

                                                      aaCCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGGAGtt                                   >  2:82212/1‑92 (MQ=255)

                                                      aaCCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTa                                   <  2:3522851/93‑1 (MQ=255)

                                                       aCCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTAt                                  >  2:1286143/1‑93 (MQ=255)

                                                       aCCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTAt                                  >  2:3818848/1‑93 (MQ=255)

                                                        ccATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTAtt                                 >  2:8819239/1‑93 (MQ=255)

                                                        ccATGATCGCCCCGATCACTGGCGGGTTACGGGTGGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTAtt                                 <  2:813996/93‑1 (MQ=255)

                                                        ccATCACCGCCCCGACCACTACGGGTTTCCTGACCGCCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTAtt                                 <  1:6522195/93‑1 (MQ=255)

                                                         cATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTc                                >  2:6999298/1‑93 (MQ=255)

                                                         cATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCCCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTc                                <  1:7028938/93‑1 (MQ=255)

                                                           tgcgcgcgCCGATCCCGGTCGGGTTACTGATCGGCCCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCgg                              <  1:3189854/89‑1 (MQ=255)

                                                           tcaccgcgCCGATCACTGTCGGTTTACTGATGGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCgg                              <  1:9319537/89‑1 (MQ=255)

                                                           tGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCgg                              >  1:6123233/1‑93 (MQ=255)

                                                           tGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCgg                              >  2:9623284/1‑93 (MQ=255)

                                                           tGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTCCCTGAGTGACGCCGCGACGCTTCACGATCCGGAAGCACTCgg                              >  2:8956349/1‑93 (MQ=255)

                                                           ggATCCCGCCGACCGTTGTGGGGTTCCTGACGGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCgg                              <  1:3947599/92‑1 (MQ=255)

                                                               cgcgCCGATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCa                          >  1:8013476/1‑93 (MQ=255)

                                                                 cgcCGATCCCTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGt                        <  1:1262384/93‑1 (MQ=255)

                                                                  gcCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTa                       >  1:954779/1‑93 (MQ=255)

                                                                    cGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTAct                     >  1:2341354/1‑93 (MQ=255)

                                                                     gATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTActc                    >  2:1470050/1‑93 (MQ=255)

                                                                      ctcgcTGTGGGGTTACTGGCCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTActct                   <  2:2585966/89‑1 (MQ=255)

                                                                      accACTGTGGGTTTCCTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTActct                   <  2:8309378/91‑1 (MQ=255)

                                                                      aTCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGNAAGTATTCGGTCCAGTActct                   >  1:7605789/1‑93 (MQ=255)

                                                                      aTCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTActct                   >  1:7737210/1‑93 (MQ=255)

                                                                      aTCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTActct                   >  1:6431392/1‑93 (MQ=255)

                                                                       cctttGTCGGTTTCCTGACCGCCCCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCtt                  <  1:1890527/89‑1 (MQ=255)

                                                                         ccTGTCGGGTTACTGAGCGGCCCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTa                <  1:8590615/92‑1 (MQ=255)

                                                                          cTGTGGGTTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTaa               <  1:6798728/93‑1 (MQ=255)

                                                                          cTGTCGTGTTACTGAGCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTaa               <  1:8377969/93‑1 (MQ=255)

                                                                          cTGTCGGTTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTaa               <  1:10021357/93‑1 (MQ=255)

                                                                          cTGTCGGGTTACTGATGGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTaa               <  2:3249128/93‑1 (MQ=255)

                                                                          cTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTaa               >  2:188665/1‑93 (MQ=255)

                                                                           tgtgGGGTTGCTGATGGGCTCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTaaa              <  2:1052106/93‑1 (MQ=255)

                                                                           tGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTaaa              >  1:176392/1‑93 (MQ=255)

                                                                           tGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTaaa              >  2:9256588/1‑93 (MQ=255)

                                                                             tCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAgc            >  2:797236/1‑93 (MQ=255)

                                                                              cGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAgcg           >  2:7077151/1‑93 (MQ=255)

                                                                               gggTTCCTGACCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAgcgc          <  2:5253094/93‑1 (MQ=255)

                                                                               gggTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAgcgc          >  1:7323966/1‑93 (MQ=255)

                                                                               gggTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAgcgc          <  1:1595860/93‑1 (MQ=255)

                                                                               gggTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAgcgc          >  1:6671564/1‑93 (MQ=255)

                                                                               gggTTACTGATCGGCGCCCGCTGCGCCTGTTACTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAgcgc          >  2:5209119/1‑93 (MQ=255)

                                                                                ggTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGcgcg         >  1:9430355/1‑93 (MQ=255)

                                                                                ggTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGcgcg         >  1:6344589/1‑93 (MQ=255)

                                                                                ggTTACTGAGCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGAACTCTTTAAAGcgcg         <  2:4934317/93‑1 (MQ=255)

                                                                                  ttACTGACCGCCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGcgga       <  1:8133032/93‑1 (MQ=255)

                                                                                   tACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGcggat      <  2:1138774/93‑1 (MQ=255)

                                                                                    aCTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGcggatc     <  1:9822830/93‑1 (MQ=255)

                                                                                      tGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGcggatcgg   >  1:8696471/1‑93 (MQ=255)

                                                                                      tGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGcggatcgg   >  2:5892891/1‑93 (MQ=255)

TCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCAC‑GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGA  >  NC_000913/2574713‑2574891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCG  >  NC_000913/2574710‑2574895

TGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGATCCATGATCGCGCCGATCACTGTCGGGTTACTGATCG                                                                                              <  SRR4302540.2771508/94‑1 (MQ=60)

TGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCGTGATCGCGCCGATCACTGTCGGGTTACTGATCG                                                                                              <  SRR4302540.4240798/94‑1 (MQ=60)

TGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCG                                                                                              >  SRR4302540.10047955/1‑94 (MQ=60)

TGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCG                                                                                              >  SRR4302540.4661922/1‑94 (MQ=60)

 GTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGG                                                                                             >  SRR4302540.6223289/1‑94 (MQ=60)

  TTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGC                                                                                            <  SRR4302540.8378925/94‑1 (MQ=60)

  TTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGC                                                                                            <  SRR4302540.4295451/94‑1 (MQ=60)

   TCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCG                                                                                           >  SRR4302540.3552231/1‑94 (MQ=60)

     ATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCC                                                                                         <  SRR4302540.7209876/94‑1 (MQ=60)

     ATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCC                                                                                         >  SRR4302540.5065956/1‑94 (MQ=60)

      TGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCC                                                                                        >  SRR4302540.6374287/1‑94 (MQ=60)

        aaaacccccccccgtcCCGCAAACCATTCCACCGGCTTCCCCACGCTGAACCATGACCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGC                                                                                      <  SRR4302540.7731235/78‑1 (MQ=60)

        ATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGC                                                                                      >  SRR4302540.8720100/1‑94 (MQ=60)

           ATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGC                                                                                   >  SRR4302540.7113373/1‑94 (MQ=60)

            CCACCCACCGTACCGCAAATCATTGCATCGGCTCCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCG                                                                                  <  SRR4302540.10077539/94‑1 (MQ=60)

             CACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGC                                                                                 >  SRR4302540.1712380/1‑94 (MQ=60)

             CACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCT‑CGCC                                                                                >  SRR4302540.7826165/1‑94 (MQ=60)

             CACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGAGCCCGCTGCGC                                                                                 >  SRR4302540.7826166/1‑94 (MQ=60)

              ACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCC                                                                                >  SRR4302540.572989/1‑94 (MQ=60)

              ACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCC                                                                                >  SRR4302540.7357924/1‑94 (MQ=60)

              ACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCC                                                                                >  SRR4302540.7028361/1‑94 (MQ=60)

               CCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTTAACCATGATCGCGCCGATCACTGTCGGGTTACTCATCTGCGCCCGCCGCCCCT                                                                               >  SRR4302540.7589541/1‑94 (MQ=60)

               CCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGACCCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCT                                                                               <  SRR4302540.6944902/94‑1 (MQ=60)

                CCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTG                                                                              >  SRR4302540.6639449/1‑94 (MQ=60)

                 CACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGT                                                                             >  SRR4302540.9210937/1‑94 (MQ=60)

                  ACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTT                                                                            >  SRR4302540.5354975/1‑94 (MQ=60)

                  ACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTT                                                                            >  SRR4302540.7416868/1‑94 (MQ=60)

                  ACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTT                                                                            >  SRR4302540.8283288/1‑94 (MQ=60)

                  ACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTT                                                                            >  SRR4302540.9493936/1‑94 (MQ=60)

                   CCGTCCCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCAGGATCGCGCCGATCCCTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTC                                                                           <  SRR4302540.5461046/94‑1 (MQ=60)

                   CCGTACCGCAAATCATTGCATGGGCTCCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGACCGGCGCCCGCTGCGCCTGTTC                                                                           <  SRR4302540.3091940/94‑1 (MQ=60)

                    CGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCC                                                                          >  SRR4302540.5986230/1‑94 (MQ=60)

                     gtcccaaaaaccatgccttcgcctcCCCCACGCGGACCCATGATCGCGCCTATCATTGTGGGGTTACTGACCGGCGCCCGCTGCGCCTGTTCCT                                                                         <  SRR4302540.4379143/69‑1 (MQ=30)

                      TACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTG                                                                        >  SRR4302540.8158783/1‑94 (MQ=60)

                         CGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGG                                                                     >  SRR4302540.2189892/1‑94 (MQ=60)

                          GCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTG                                                                    >  SRR4302540.4048414/1‑94 (MQ=60)

                            AAATCATTGCATGGGCTTCCCCCCGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGAC                                                                  <  SRR4302540.8741954/94‑1 (MQ=60)

                            AAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGAC                                                                  >  SRR4302540.2682295/1‑94 (MQ=60)

                            AAATCATTGCATCGGCTCCCCCCCGCTGACCCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGAC                                                                  <  SRR4302540.6314068/94‑1 (MQ=60)

                            AAATCATTGCATCGGCTCCCCCCCGCTGAACCATGACCGCGCCGATCACGGTGGGGTTCCTGATCGCCGCCCGCTGCGCCTGTTCCTGAGTGAC                                                                  <  SRR4302540.853334/94‑1 (MQ=60)

                            AAATCATTGCATCGCCCCCCCCACGCTAACCCATGCCCGCGCCGATCCCTGTGGGGTTACTGATCGGCCCCCGCTGCGCCTGTTCCTGAGTGAC                                                                  <  SRR4302540.7262711/94‑1 (MQ=32)

                             attcggtgcgtccgcctcgccgcgcacaaccaggagcggGCCGAGCACTGTCGGGTTACTAACCGGCGCCCGCTGCGCCTGTTCCTGAGTGACG                                                                 <  SRR4302540.1281508/55‑1 (MQ=40)

                               TCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCC                                                               <  SRR4302540.1706790/94‑1 (MQ=60)

                                  TTGCTTCGGCTCCCCCACGCTGACCCATGACCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCG                                                            <  SRR4302540.9408925/94‑1 (MQ=60)

                                  TTGCAGCGGCTCCCCCCCGCTGACCCATGATCGCGCCGATCACGGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCG                                                            <  SRR4302540.773596/94‑1 (MQ=60)

                                   TGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGA                                                           >  SRR4302540.4435768/1‑94 (MQ=60)

                                      ATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGC                                                        >  SRR4302540.1296727/1‑94 (MQ=60)

                                      ATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGC                                                        >  SRR4302540.3697099/1‑94 (MQ=60)

                                       TCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCT                                                       <  SRR4302540.1585536/94‑1 (MQ=60)

                                       TCGGCTCCCCCCCGCTGAACCAGGATCGCGCCGATCCCTGTGGGGTTATTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCT                                                       <  SRR4302540.9876402/94‑1 (MQ=60)

                                         GGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTC                                                     >  SRR4302540.8639774/1‑94 (MQ=60)

                                         GGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTC                                                     >  SRR4302540.4046486/1‑94 (MQ=60)

                                           CTCCCCCCCGCTGAACCATGACCGCGCGGATCACTGTGGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCAT                                                   <  SRR4302540.5139552/94‑1 (MQ=60)

                                           CTCCCCCACGGTAAACCATGACCGCGCCTATCTCTGGCGGTTTACTGGGCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCAT                                                   <  SRR4302540.3096768/94‑1 (MQ=40)

                                            TTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATG                                                  >  SRR4302540.4905987/1‑94 (MQ=60)

                                             TCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATCA                                                 >  SRR4302540.2156640/1‑94 (MQ=60)

                                             CCCCCCCGCTGACCCATGACCGCGCGGATCACTGGCGGGTTACTGACGGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGA                                                 <  SRR4302540.2396232/94‑1 (MQ=60)

                                             CCCCCACGCTGAACCATGATCGCGCCGCTCACTGTGGGGTTACTGTTCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGA                                                 <  SRR4302540.5284796/94‑1 (MQ=60)

                                               CCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATC                                               >  SRR4302540.4885934/1‑94 (MQ=60)

                                                CCACGCTGACCCAGGATCGCGCCGATCGCTGTGGGGTTACGGAGCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCT                                              <  SRR4302540.7749557/94‑1 (MQ=60)

                                                 CACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTG                                             >  SRR4302540.9922910/1‑94 (MQ=60)

                                                 CACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTG                                             >  SRR4302540.5608528/1‑94 (MQ=60)

                                                 CACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTG                                             >  SRR4302540.5608527/1‑94 (MQ=60)

                                                 CACGCTGAACCATGATCGCCCCGATCACTGTCGGGGTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTG                                             >  SRR4302540.9922911/1‑94 (MQ=60)

                                                  CCGCTGACCCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGG                                            <  SRR4302540.7093062/94‑1 (MQ=60)

                                                  ACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGG                                            >  SRR4302540.10061959/1‑94 (MQ=60)

                                                   CGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGA                                           >  SRR4302540.7181905/1‑94 (MQ=60)

                                                    GCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAA                                          >  SRR4302540.128923/1‑94 (MQ=60)

                                                     CTGACCCATGATCGCGCCGATCCCTGGCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAG                                         <  SRR4302540.5428832/94‑1 (MQ=60)

                                                      tgacccaggacggggcgGATCACTGTCGGGTTGCTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGT                                        <  SRR4302540.8748378/77‑1 (MQ=60)

                                                      TGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGT                                        >  SRR4302540.6411558/1‑94 (MQ=60)

                                                         ACCATGGTCGCCCCGATCCCTGTGGGTTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATT                                     <  SRR4302540.7754077/94‑1 (MQ=60)

                                                           CATGGTCGCGGCGATCACTGTCGGGTTACTGCTCGGCGCCCGCTGCGCCTGTCCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCG                                   <  SRR4302540.7107039/94‑1 (MQ=60)

                                                           CATGGTCGCGCCGATCCCTGTGGGGTTATTACTCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCG                                   <  SRR4302540.5071458/94‑1 (MQ=60)

                                                           CATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCCCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCG                                   <  SRR4302540.7107037/94‑1 (MQ=60)

                                                            agcacCGCGCCGATCACTGGCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGACTGACGCCGCGACCCTTCATGATCTGGAAGTATTCGG                                  <  SRR4302540.1789582/89‑1 (MQ=60)

                                                            ATGATCGCGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGG                                  >  SRR4302540.6191426/1‑94 (MQ=60)

                                                             tgcgCGCGCCGATCCCGGTCGGGTTACTGATCGGCCCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGT                                 <  SRR4302540.3224504/90‑1 (MQ=60)

                                                             tcacCGCGCCGATCACTGTCGGTTTACTGATGGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGT                                 <  SRR4302540.9430737/90‑1 (MQ=60)

                                                             TGATCGCGCCGATCACTGTGGGGTTACGGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGT                                 <  SRR4302540.3061320/94‑1 (MQ=60)

                                                               cgcgcgccgatccgggtgGGGTTACTGGTCGGCCCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCC                               <  SRR4302540.389424/76‑1 (MQ=60)

                                                               accccccgtatctttgggggtTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCC                               <  SRR4302540.3835227/73‑1 (MQ=60)

                                                                TCGCGCCGATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCA                              >  SRR4302540.8106687/1‑94 (MQ=60)

                                                                 CGCGCCGATCACTGTCGGTTTACTGATCGGCCCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAG                             <  SRR4302540.1801659/94‑1 (MQ=60)

                                                                   CGCCGATCCCTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTA                           <  SRR4302540.1275345/94‑1 (MQ=60)

                                                                   CGCCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTA                           >  SRR4302540.964679/1‑94 (MQ=60)

                                                                     CCGATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACT                         >  SRR4302540.2366586/1‑94 (MQ=60)

                                                                       GATCCCTGGCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCT                       <  SRR4302540.3172773/94‑1 (MQ=60)

                                                                       GATCACTGTCGGGTTACTGATCGGCGCCCGCTGGGCCTGTTCCTGAGTGAGGCCGAGACGCTTCATGATCTGNAAGTATTCGGTCCAGTACTCT                       >  SRR4302540.7691258/1‑94 (MQ=60)

                                                                       GATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGNAAGTATTCGGTCCAGTACTCT                       >  SRR4302540.7691257/1‑94 (MQ=60)

                                                                       GATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCT                       >  SRR4302540.8868536/1‑94 (MQ=60)

                                                                       GATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCT                       >  SRR4302540.6502977/1‑94 (MQ=60)

                                                                       GATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCT                       >  SRR4302540.7827538/1‑94 (MQ=60)

                                                                       GATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCT                       >  SRR4302540.4513442/1‑94 (MQ=60)

                                                                       GATCACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGGATTCGGTCCAGTACTCT                       >  SRR4302540.7827539/1‑94 (MQ=60)

                                                                          CACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTA                    >  SRR4302540.2133860/1‑94 (MQ=60)

                                                                          CACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTA                    >  SRR4302540.4385023/1‑94 (MQ=60)

                                                                           CCTGTCGGGTTACTGAGCGGCCCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAA                   <  SRR4302540.8690483/94‑1 (MQ=60)

                                                                           ACTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAA                   >  SRR4302540.6735684/1‑94 (MQ=60)

                                                                            CTGTGGGTTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAA                  <  SRR4302540.6873934/94‑1 (MQ=60)

                                                                            CTGTCGTGTTACTGAGCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAA                  <  SRR4302540.8475187/94‑1 (MQ=60)

                                                                            CTGTCGGTTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAA                  <  SRR4302540.10137980/94‑1 (MQ=60)

                                                                            CTGTCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAA                  >  SRR4302540.178150/1‑94 (MQ=60)

                                                                             TGCCGGGTTACTGACGGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAG                 <  SRR4302540.9619479/94‑1 (MQ=60)

                                                                              gttggtttctTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGC                <  SRR4302540.7089079/84‑1 (MQ=60)

                                                                               TCGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCG               >  SRR4302540.9290148/1‑94 (MQ=60)

                                                                                CGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGC              >  SRR4302540.7405758/1‑94 (MQ=60)

                                                                                CGGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGC              >  SRR4302540.6745278/1‑94 (MQ=60)

                                                                                 GGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCG             <  SRR4302540.1613298/94‑1 (MQ=60)

                                                                                 GGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCG             >  SRR4302540.6415238/1‑94 (MQ=60)

                                                                                 GGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCG             >  SRR4302540.6415237/1‑94 (MQ=60)

                                                                                 GGGTTACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCG             >  SRR4302540.9542382/1‑94 (MQ=60)

                                                                                   GTTACTGCGCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGA           <  SRR4302540.6233151/94‑1 (MQ=60)

                                                                                    TTACTGACCGCCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGAT          <  SRR4302540.8227429/94‑1 (MQ=60)

                                                                                      ACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCG        >  SRR4302540.6624663/1‑94 (MQ=60)

                                                                                      ACTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCG        <  SRR4302540.9937750/94‑1 (MQ=60)

                                                                                       CTGATCGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGG       >  SRR4302540.8797427/1‑94 (MQ=60)

                                                                                            CGGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCG  <  SRR4302540.3473599/94‑1 (MQ=60)

TGTTCATGATAATCACCCACCGTACCGCAAATCATTGCATCGGCTTCCCCACGCTGAACCATGATCGCGCCGATCACTGTCGGGTTACTGATCAGCGCCCGCTGCGCCTGTTCCTGAGTGACGCCGCGACGCTTCATGATCTGGAAGTATTCGGTCCAGTACTCTTTAAAGCGCGGATCGGATTCG  >  NC_000913/2574710‑2574895

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`