Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,579,447 T→C S598P (TCG→CCG)  tktB → transketolase 2 isozyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,579,4470TC99.6% 774.4 / ‑4.9 244S598P (TCG→CCG) tktBtransketolase 2 isozyme
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (125/118);  total (125/119)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

Only 100 of 250 total aligned reads displayed.

GAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGC  >  NC_000913/2579357‑2579535
                                                                                          |                                                                                         
gagaAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCg                                                                                         >  1:143272/1‑93 (MQ=255)
gagaAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCg                                                                                         >  2:958120/1‑93 (MQ=255)
gagaAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCg                                                                                         >  2:8670476/1‑93 (MQ=255)
  gaAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGgt                                                                                       >  1:3566441/1‑93 (MQ=255)
   aaaTTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGgtg                                                                                      <  2:2323658/93‑1 (MQ=255)
    aaTTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGgtgt                                                                                     <  2:1700546/93‑1 (MQ=255)
    aaTTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGgtgt                                                                                     >  2:8749649/1‑93 (MQ=255)
           aGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTc                                                                              <  1:5876132/93‑1 (MQ=255)
            ggAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCt                                                                             >  2:3224506/1‑93 (MQ=255)
            ggAGAAGGACGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGGCTATCGGGAGCCGGTGTTGGCTTCt                                                                             >  2:8244473/1‑93 (MQ=255)
             gagaAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGGCTTCTa                                                                            >  2:7995788/1‑93 (MQ=255)
             gagaAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTa                                                                            >  1:1701603/1‑93 (MQ=255)
              agaAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTaa                                                                           >  2:6640578/1‑93 (MQ=255)
              agaAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTaa                                                                           <  1:2027166/93‑1 (MQ=255)
              agaAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTaa                                                                           <  2:5272154/93‑1 (MQ=255)
              agaAGGTAGCAATGTACGAGTAGTTTCCCTGCCCTCGACAGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTaa                                                                           <  2:838642/93‑1 (MQ=255)
                aaGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACg                                                                         <  2:4133456/93‑1 (MQ=255)
                 aGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGt                                                                        >  2:802050/1‑93 (MQ=255)
                 aGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGt                                                                        >  1:5707012/1‑93 (MQ=255)
                  ggTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGtt                                                                       >  1:3006596/1‑93 (MQ=255)
                   gTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTg                                                                      >  2:9908278/1‑93 (MQ=255)
                       cAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGc                                                                  >  2:2527709/1‑93 (MQ=255)
                        aaTGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCt                                                                 >  2:3618676/1‑93 (MQ=255)
                        aaTGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCt                                                                 <  1:9490409/93‑1 (MQ=255)
                         aTGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTc                                                                <  1:3072330/93‑1 (MQ=255)
                         aTGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTc                                                                <  2:2256/93‑1 (MQ=255)
                          tGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTcc                                                               >  2:6143298/1‑92 (MQ=255)
                           gTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGCATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTcgg                                                              >  1:1911803/1‑92 (MQ=255)
                            tACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTTCGGCTcgc‑g                                                            >  1:702170/1‑93 (MQ=255)
                            tACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTcgc‑g                                                            >  2:3002965/1‑93 (MQ=255)
                             aCGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑Gt                                                           <  1:7873039/93‑1 (MQ=255)
                              cgcgTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTg                                                          <  2:1964637/93‑1 (MQ=255)
                              cgcgTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTg                                                          <  1:9729763/93‑1 (MQ=255)
                               gcgTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑CTgg                                                         >  1:9612204/1‑93 (MQ=255)
                                  tAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCgg                                                      >  2:5062007/1‑93 (MQ=255)
                                  tAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCgg                                                      <  1:1012380/93‑1 (MQ=255)
                                     tttCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGgggg                                                   >  1:430450/1‑93 (MQ=255)
                                     tttCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCCC‑GTGGCGGTgg                                                   >  1:4087892/1‑93 (MQ=255)
                                       tCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGaa                                                 <  1:6402720/93‑1 (MQ=255)
                                       tCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGaa                                                 <  2:6943205/93‑1 (MQ=255)
                                        cccTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGagg                                                >  1:3191240/1‑91 (MQ=255)
                                        cccTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAg                                                <  1:7188520/93‑1 (MQ=255)
                                          cttcccTCGACCGATATTTTCCACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCa                                              <  1:2811522/90‑1 (MQ=255)
                                          cTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCa                                              <  1:7297711/93‑1 (MQ=255)
                                           tGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAg                                             <  2:5780955/93‑1 (MQ=255)
                                           tGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAg                                             <  1:4604304/93‑1 (MQ=255)
                                           tGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGAGTTGCCTTCTAACGTTGCGGCTCGC‑GCGGGGGGGGGAGCAg                                             >  1:9618108/1‑93 (MQ=255)
                                            gCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGGAGCAgg                                            >  2:5938687/1‑93 (MQ=255)
                                             cccTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGt                                           <  1:4184491/93‑1 (MQ=255)
                                              ccTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTa                                          <  1:1051363/93‑1 (MQ=255)
                                              ccTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTa                                          <  2:3781069/93‑1 (MQ=255)
                                               cTCGACCGTTATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCTCGGTGGGGGT‑GAAGCAGCTAt                                         >  2:8630047/1‑93 (MQ=255)
                                               cTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGGGGGAGCAGGGAt                                         >  1:4729622/1‑93 (MQ=255)
                                                tCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGGAtt                                        >  1:2316811/1‑93 (MQ=255)
                                                tCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGGGGGAGGAGGGAtt                                        >  2:9765928/1‑93 (MQ=255)
                                                   aCCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGGAGCAGGTATTGcc                                     >  2:6411830/1‑93 (MQ=255)
                                                   aCCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGGGGGAGGAGGTATTGcc                                     >  2:5417669/1‑93 (MQ=255)
                                                   aCCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGGGGGAGCAGGGATTGcc                                     >  2:430294/1‑93 (MQ=255)
                                                    ccGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGGGGGAGCAGGGATTGCCg                                    >  1:3246184/1‑93 (MQ=255)
                                                     cGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCgg                                   >  1:525991/1‑92 (MQ=255)
                                                      gATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGGAGCAGGTATTGCCGAt                                  >  1:8412336/1‑93 (MQ=255)
                                                       atatTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGGGGGAGCAGGTATTGCCGAtt                                 >  2:7405299/1‑93 (MQ=255)
                                                        tatTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTa                                <  2:2871580/93‑1 (MQ=255)
                                                        tatTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTa                                <  1:9269911/93‑1 (MQ=255)
                                                        tatTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGGGGAAGCAGGTATTGCCGATTa                                >  1:9138076/1‑93 (MQ=255)
                                                          ttttCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGGAGCAGGTATTGCCGATTACt                              >  1:1441257/1‑93 (MQ=255)
                                                          ttttCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGAGGAAGCAGGTATTGCCGATTACt                              >  2:4974659/1‑93 (MQ=255)
                                                             tCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGt                           <  2:7685930/93‑1 (MQ=255)
                                                              cGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTa                          <  1:6006197/93‑1 (MQ=255)
                                                                aCGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACa                        <  2:6210538/93‑1 (MQ=255)
                                                                  gCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAg                      <  1:7280608/93‑1 (MQ=255)
                                                                  gCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAg                      <  2:118475/93‑1 (MQ=255)
                                                                   cccAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGt                     >  2:1152556/1‑93 (MQ=255)
                                                                    ccAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTa                    <  2:1468444/93‑1 (MQ=255)
                                                                    ccAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTa                    <  1:857090/93‑1 (MQ=255)
                                                                     cAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGGAt                   >  1:3812446/1‑93 (MQ=255)
                                                                     cAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑CGGGCGGTGGGAGCAGGCGTTGCCGATTTCTGGAACCAGTAt                   >  1:2566924/1‑93 (MQ=255)
                                                                       ggATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGGATTGCCGATTACTGGTACAAGTATGt                 >  2:7692933/1‑93 (MQ=255)
                                                                       ggATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGGATTGCCGATTACTGGTACAAGTATGt                 >  1:6784554/1‑93 (MQ=255)
                                                                       ggATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGGATTGCCGATTACTGGGACAAGTATGt                 >  1:9378196/1‑93 (MQ=255)
                                                                        gATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGtt                <  1:6297646/93‑1 (MQ=255)
                                                                        gATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGtt                <  2:2498664/93‑1 (MQ=255)
                                                                         aTGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTg               <  1:4671762/93‑1 (MQ=255)
                                                                         aTGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGGATTGCCGATTACTGGTACAAGTATGTTg               >  1:9849898/1‑93 (MQ=255)
                                                                          tGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTgg              <  1:3960260/93‑1 (MQ=255)
                                                                          tGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGGATTGCCGATTACTGGTACAAGTATGTTgg              >  1:2518005/1‑93 (MQ=255)
                                                                           gAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGt             <  1:8882530/93‑1 (MQ=255)
                                                                            aGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTc            >  1:9033535/1‑93 (MQ=255)
                                                                               aaTATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTTGTCTGa         >  2:3156177/1‑93 (MQ=255)
                                                                               aaTATCGGGAGCCGGTGGTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGa         <  1:7074950/93‑1 (MQ=255)
                                                                                atatCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGaa        <  1:4512826/93‑1 (MQ=255)
                                                                                atatCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGaa        <  1:8279138/93‑1 (MQ=255)
                                                                                 tatCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGaaa       >  2:7033245/1‑93 (MQ=255)
                                                                                 tatCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGaaa       >  1:2158620/1‑93 (MQ=255)
                                                                                 tatCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGGATGTTGGGCTGaaa       >  2:1376617/1‑93 (MQ=255)
                                                                                 tatCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGGATTGCCGATTACTGGTACAAGGATGTTGGTCTGaaa       >  1:59485/1‑93 (MQ=255)
                                                                                 tatCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGGGGGAGCAGGTATTGCCGGTTACTTGTTCCAGCATGGTGGGCTGaaa       >  2:7479234/1‑93 (MQ=255)
                                                                                  atCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAg      <  2:8744229/93‑1 (MQ=255)
                                                                                  atCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAg      <  2:9320714/93‑1 (MQ=255)
                                                                                      ggAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGc  <  1:5075063/93‑1 (MQ=255)
                                                                                          |                                                                                         
GAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGC‑GTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGC  >  NC_000913/2579357‑2579535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTG  >  NC_000913/2579355‑2579540
                                                                                            |                                                                                             
CAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCC                                                                                              >  SRR4302540.4563283/1‑94 (MQ=60)
 AGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCG                                                                                             >  SRR4302540.2278730/1‑94 (MQ=60)
 AGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCG                                                                                             >  SRR4302540.144763/1‑94 (MQ=60)
 AGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCG                                                                                             >  SRR4302540.144764/1‑94 (MQ=60)
 AGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCG                                                                                             >  SRR4302540.3138940/1‑94 (MQ=60)
 AGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCG                                                                                             <  SRR4302540.1726449/94‑1 (MQ=60)
   AGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGT                                                                                           >  SRR4302540.2552388/1‑94 (MQ=60)
   AGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGT                                                                                           >  SRR4302540.3605402/1‑94 (MQ=60)
   AGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGT                                                                                           <  SRR4302540.9695555/94‑1 (MQ=60)
    GAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTG                                                                                          <  SRR4302540.3263746/94‑1 (MQ=60)
     AAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGT                                                                                         <  SRR4302540.8580461/94‑1 (MQ=60)
      AATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTT                                                                                        <  SRR4302540.4069276/94‑1 (MQ=60)
       ATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTG                                                                                       <  SRR4302540.5521911/94‑1 (MQ=60)
        TTAGCATGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGC                                                                                      <  SRR4302540.8704431/94‑1 (MQ=60)
          AGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCT                                                                                    >  SRR4302540.1531300/1‑94 (MQ=60)
          AGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCT                                                                                    >  SRR4302540.4368386/1‑94 (MQ=60)
            CAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTC                                                                                  >  SRR4302540.3174308/1‑94 (MQ=60)
            CAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGACGAGGAATATCGGGAGCCTGTGTTCCCTTC                                                                                  >  SRR4302540.5967370/1‑94 (MQ=60)
             AGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCT                                                                                 >  SRR4302540.1693200/1‑94 (MQ=60)
             AGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCT                                                                                 <  SRR4302540.5941085/94‑1 (MQ=60)
             AGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCT                                                                                 <  SRR4302540.7885843/94‑1 (MQ=60)
              GGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTA                                                                                >  SRR4302540.1720024/1‑94 (MQ=60)
              GGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTA                                                                                <  SRR4302540.7111935/94‑1 (MQ=60)
                AGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAAC                                                                              <  SRR4302540.2049059/94‑1 (MQ=60)
                AGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAAC                                                                              <  SRR4302540.7440777/94‑1 (MQ=60)
                 GAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACG                                                                             <  SRR4302540.1395206/94‑1 (MQ=60)
                  AAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGT                                                                            >  SRR4302540.5769842/1‑94 (MQ=60)
                   AGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGTAGCCGGTGTTGCCTTCTAACGTT                                                                           >  SRR4302540.4826710/1‑94 (MQ=60)
                   AGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTT                                                                           >  SRR4302540.3039693/1‑94 (MQ=60)
                      TCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCG                                                                        <  SRR4302540.6734572/94‑1 (MQ=60)
                          AATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTC                                                                    >  SRR4302540.2440534/1‑94 (MQ=60)
                          AATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTC                                                                    >  SRR4302540.8420082/1‑94 (MQ=60)
                          AATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTC                                                                    <  SRR4302540.9602914/94‑1 (MQ=60)
                           ATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCG                                                                   <  SRR4302540.10093663/94‑1 (MQ=60)
                           ATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCG                                                                   <  SRR4302540.3106040/94‑1 (MQ=60)
                           ATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCC                                                                   >  SRR4302540.1191436/1‑94 (MQ=60)
                            TGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGCATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGG                                                                  >  SRR4302540.1932689/1‑94 (MQ=60)
                             GTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTTCGGCTCGCG                                                                 >  SRR4302540.709706/1‑94 (MQ=60)
                             GTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCG                                                                 <  SRR4302540.3967105/94‑1 (MQ=60)
                               ACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTG                                                               <  SRR4302540.7965004/94‑1 (MQ=60)
                               ACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTG                                                               <  SRR4302540.9731318/94‑1 (MQ=60)
                               ACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTG                                                               <  SRR4302540.9733906/94‑1 (MQ=60)
                                CGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGG                                                              <  SRR4302540.9844021/94‑1 (MQ=60)
                                CGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCCTGG                                                              >  SRR4302540.9725539/1‑94 (MQ=60)
                                 GCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCccggggg                                                             >  SRR4302540.2794470/1‑87 (MQ=60)
                                 GCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGC                                                             <  SRR4302540.4672195/94‑1 (MQ=60)
                                    TAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGgggg                                                          >  SRR4302540.5823870/1‑90 (MQ=60)
                                    TAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGT                                                          <  SRR4302540.1022642/94‑1 (MQ=60)
                                     AGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTG                                                         <  SRR4302540.9646657/94‑1 (MQ=60)
                                      GTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGGGG                                                        >  SRR4302540.3821789/1‑94 (MQ=60)
                                      GTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGGGG                                                        >  SRR4302540.434999/1‑94 (MQ=60)
                                      GTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCCCGTGGCGGTGG                                                        >  SRR4302540.4131978/1‑94 (MQ=60)
                                       TTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGA                                                       <  SRR4302540.5996995/94‑1 (MQ=60)
                                       TTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCCCGTGGggggggg                                                       >  SRR4302540.7323736/1‑87 (MQ=60)
                                        TTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAA                                                      <  SRR4302540.4577193/94‑1 (MQ=60)
                                         TCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAGG                                                     >  SRR4302540.3225904/1‑94 (MQ=60)
                                         TCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAG                                                     <  SRR4302540.6473990/94‑1 (MQ=60)
                                          CCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGC                                                    <  SRR4302540.7268651/94‑1 (MQ=60)
                                          CCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGGGGGAGC                                                    >  SRR4302540.7375488/1‑94 (MQ=60)
                                            CTTCCCTCGACCGATATTTTCCACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAG                                                  <  SRR4302540.2841882/94‑1 (MQ=60)
                                            CTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAG                                                  <  SRR4302540.2841883/94‑1 (MQ=60)
                                            CTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAG                                                  <  SRR4302540.7379160/94‑1 (MQ=60)
                                            CTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAG                                                  <  SRR4302540.465668/94‑1 (MQ=60)
                                            CTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGAGTTGCCTTCTAACGTTGCGGCTCGCGcgggggggggagcag                                                  >  SRR4302540.9731493/1‑79 (MQ=60)
                                             TGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGG                                                 <  SRR4302540.4653764/94‑1 (MQ=60)
                                             TGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGG                                                 <  SRR4302540.4144253/94‑1 (MQ=60)
                                              GCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGT                                                <  SRR4302540.4218612/94‑1 (MQ=60)
                                               CCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTA                                               <  SRR4302540.4229962/94‑1 (MQ=60)
                                               CCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGGGGAAGCAGGTA                                               >  SRR4302540.5140077/1‑94 (MQ=60)
                                                CCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTAT                                              <  SRR4302540.4748971/94‑1 (MQ=60)
                                                CCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTAT                                              <  SRR4302540.1062060/94‑1 (MQ=60)
                                                CCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTAT                                              >  SRR4302540.3088156/1‑94 (MQ=60)
                                                CCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGGGGGAGCAGGGAT                                              >  SRR4302540.4780131/1‑94 (MQ=60)
                                                CCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGGGGCGGggggaggcgggat                                              >  SRR4302540.2183416/1‑81 (MQ=60)
                                                CCTCGACCGATATTTACGACGCACAGGATGAGGAATATCGGAAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTAT                                              <  SRR4302540.9061632/94‑1 (MQ=60)
                                                 CTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGGATT                                             >  SRR4302540.2341754/1‑94 (MQ=60)
                                                    GACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCC                                          <  SRR4302540.3899073/94‑1 (MQ=60)
                                                     ACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCG                                         <  SRR4302540.4925630/94‑1 (MQ=60)
                                                     ACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGGGGGAGCAGGGATTGCCG                                         >  SRR4302540.3281530/1‑94 (MQ=60)
                                                      CCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGG                                        >  SRR4302540.531675/1‑94 (MQ=60)
                                                       CGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGGAGCAGGTATTGCCGAT                                       >  SRR4302540.8509978/1‑94 (MQ=60)
                                                        GATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGGATTGCCcgtt                                      >  SRR4302540.3414582/1‑90 (MQ=60)
                                                         ATATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGGGGAAGCAGGTATTGCCGATTA                                     >  SRR4302540.9247874/1‑94 (MQ=60)
                                                          TATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTAC                                    <  SRR4302540.9380764/94‑1 (MQ=60)
                                                           ATTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGGAGCAGGTATTGCCGATTACT                                   >  SRR4302540.1456903/1‑94 (MQ=60)
                                                            TTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGGAGCAGGTATTGCCGATTACTG                                  >  SRR4302540.6420778/1‑94 (MQ=60)
                                                            TTTTCGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTG                                  <  SRR4302540.7150845/94‑1 (MQ=60)
                                                                CGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTAC                              <  SRR4302540.1970216/94‑1 (MQ=60)
                                                                CGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTAC                              <  SRR4302540.6072786/94‑1 (MQ=60)
                                                                CGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGCGGCGGTGGAAGCAGAGATTGCCGATTATTGGTAA                              >  SRR4302540.2151209/1‑94 (MQ=60)
                                                                AGACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTAC                              <  SRR4302540.9155229/94‑1 (MQ=60)
                                                                 GACGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACA                             <  SRR4302540.3574454/94‑1 (MQ=60)
                                                                 GACGCCCAGGATGAGGAATATCGGGAGCCGGGGTTGCCTTCTAACGTTGCGGCTCCCGTGGCGGGGGGAGAAGGTATTGGCGATTACTGGTACA                             >  SRR4302540.9295022/1‑94 (MQ=60)
                                                                   CGCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGGGGAAGCAGGTATTGCCGATTACTGGTACACG                           >  SRR4302540.8845626/1‑94 (MQ=60)
                                                                    GCCCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGT                          <  SRR4302540.7361848/94‑1 (MQ=60)
                                                                      CCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTAT                        <  SRR4302540.5638080/94‑1 (MQ=60)
                                                                      CCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTAT                        <  SRR4302540.866193/94‑1 (MQ=60)
                                                                      CCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGGAT                        >  SRR4302540.3853961/1‑94 (MQ=60)
                                                                      CCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGGGGCGGTGGAAGCAGGGATTGCCGATTACTGGGACAAGTAT                        >  SRR4302540.9925771/1‑94 (MQ=60)
                                                                      CCAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCCGGGCGGTGGGAGCAGGCGTTGCCGATTTCTGGAACCAGTAT                        >  SRR4302540.2594597/1‑94 (MQ=55)
                                                                       CAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATG                       <  SRR4302540.1992612/94‑1 (MQ=60)
                                                                       CAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATG                       <  SRR4302540.2856596/94‑1 (MQ=60)
                                                                       CAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGGGGAAGCAGGGATTGCCGATTACTGGTACAAGTATG                       >  SRR4302540.2298110/1‑94 (MQ=60)
                                                                       CAGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTAGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATG                       >  SRR4302540.428696/1‑94 (MQ=60)
                                                                        AGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGGATTGCCGATTACTGGTACAAGTATGT                      >  SRR4302540.6859575/1‑94 (MQ=60)
                                                                        AGGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGGATTGCCGATTACTGGGACAAGTATGT                      >  SRR4302540.9489831/1‑94 (MQ=60)
                                                                         GGATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTT                     <  SRR4302540.9466/94‑1 (MQ=60)
                                                                          GATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTG                    <  SRR4302540.9766312/94‑1 (MQ=60)
                                                                          GATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTG                    <  SRR4302540.6367773/94‑1 (MQ=60)
                                                                          GATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGGATTGCCGATTACTGGTACAAGTATGTTG                    >  SRR4302540.9965031/1‑94 (MQ=60)
                                                                           ATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGGAGCAGGGATTGCCGATTACTGGTACAAGGATGTTGG                   >  SRR4302540.62515/1‑94 (MQ=60)
                                                                           ATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGG                   <  SRR4302540.4721762/94‑1 (MQ=60)
                                                                           ATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGG                   <  SRR4302540.7241218/94‑1 (MQ=60)
                                                                           ATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGG                   <  SRR4302540.9430333/94‑1 (MQ=60)
                                                                           ATGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGGATTGCCGATTACTGGTACAAGTATGTTGG                   >  SRR4302540.2545198/1‑94 (MQ=60)
                                                                            TGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCcaggcggtgggatcaggggttgaccattactggttccagtatggtggg                  >  SRR4302540.7306401/1‑46 (MQ=60)
                                                                            TGAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGT                  <  SRR4302540.4002977/94‑1 (MQ=60)
                                                                             GAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTC                 <  SRR4302540.8985332/94‑1 (MQ=60)
                                                                             GAGGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTC                 >  SRR4302540.9141945/1‑94 (MQ=60)
                                                                               GGAATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGGATGTTGGTCTG               >  SRR4302540.9565343/1‑94 (MQ=60)
                                                                                 AATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAA             <  SRR4302540.1032857/94‑1 (MQ=60)
                                                                                 AATATCGGGAGCCGGTGGTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAA             <  SRR4302540.7153678/94‑1 (MQ=60)
                                                                                  ATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGggggtatggcctatcactggtgcccggcttgtgggtggcaa            >  SRR4302540.60363/1‑53 (MQ=60)
                                                                                  ATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACCAGTATGTTGGTCTGAAA            >  SRR4302540.921751/1‑94 (MQ=60)
                                                                                  ATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAA            >  SRR4302540.4498307/1‑94 (MQ=60)
                                                                                  ATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAA            <  SRR4302540.4561721/94‑1 (MQ=60)
                                                                                  ATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAA            >  SRR4302540.2181864/1‑94 (MQ=60)
                                                                                  ATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAA            <  SRR4302540.8375299/94‑1 (MQ=60)
                                                                                  ATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGGATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAA            >  SRR4302540.6283031/1‑94 (MQ=60)
                                                                                  ATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGGATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAA            >  SRR4302540.60364/1‑94 (MQ=60)
                                                                                  ATATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGGATTGCCGATTACTGGTACAAGGATGTTGGTCTGAAA            >  SRR4302540.60087/1‑94 (MQ=60)
                                                                                   TATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAG           <  SRR4302540.7037648/94‑1 (MQ=60)
                                                                                   TATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAG           <  SRR4302540.6725458/94‑1 (MQ=60)
                                                                                   TATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAG           <  SRR4302540.8355875/94‑1 (MQ=60)
                                                                                   TATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGGATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAG           >  SRR4302540.5962704/1‑94 (MQ=60)
                                                                                    ATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGCGGCGGTGGAAGCAGGAATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGG          >  SRR4302540.2577337/1‑94 (MQ=60)
                                                                                    ATCGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCCGGTGGCGGTGGAAGCAGGCATTGTCGATTACTGGTTCGAGTATGTGGGTCTGcaggg          >  SRR4302540.7536979/1‑89 (MQ=55)
                                                                                      CGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGG        <  SRR4302540.8496915/94‑1 (MQ=60)
                                                                                      CGGGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGGATGTTGGTCTGAAAGGGG        >  SRR4302540.1725356/1‑94 (MQ=60)
                                                                                        GGAGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCA      <  SRR4302540.5129931/94‑1 (MQ=60)
                                                                                          AGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGAATAATGGTACAAGTATGTTTGTCTGAAAGGGGAAAT    >  SRR4302540.7240690/1‑94 (MQ=60)
                                                                                          AGCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGGATTGCCGATTACTGGTACAAGGATGTTGGTCTGAAAGGGGCAAT    >  SRR4302540.3961344/1‑94 (MQ=60)
                                                                                           GCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATT   <  SRR4302540.5630463/94‑1 (MQ=60)
                                                                                           GCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATT   <  SRR4302540.8772857/94‑1 (MQ=60)
                                                                                           GCCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATT   <  SRR4302540.8353082/94‑1 (MQ=60)
                                                                                            CCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTG  <  SRR4302540.9870552/94‑1 (MQ=60)
                                                                                            |                                                                                             
CAGAGAAATTAGCAGGAGAAGGTCGCAATGTACGCGTAGTTTCCCTGCCCTCGACCGATATTTTCGACGCCCAGGATGAGGAATATCGGGAGTCGGTGTTGCCTTCTAACGTTGCGGCTCGCGTGGCGGTGGAAGCAGGTATTGCCGATTACTGGTACAAGTATGTTGGTCTGAAAGGGGCAATTG  >  NC_000913/2579355‑2579540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑