Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,631,832 (T)6→5 coding (259/1467 nt) guaB ← IMP dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,631,8270T.99.7% 1395.5 / ‑3.1 308coding (264/1467 nt)guaBIMP dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base . (152/155);  total (153/155)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01

Only 100 of 337 total aligned reads displayed.

GCAAAACCGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGTTTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAA  >  NC_000913/2631735‑2631915
                                                                                            |                                                                                        
gCAAAACCGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCgtgt                                                                                          >  1:2700546/1‑93 (MQ=255)
 cAAAACCGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGtt                                                                                         >  1:5104267/1‑93 (MQ=255)
     aCCGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCCGCTTCGTGtttttc                                                                                     >  2:572514/1‑92 (MQ=255)
        gTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGtttttcaca                                                                                  <  1:598062/93‑5 (MQ=255)
        gTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGtttttcaca                                                                                  >  1:2189419/1‑89 (MQ=255)
         ttACGCTCGGTCAGTTCTTTCACTTAGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCacac                                                                                >  1:4930464/1‑93 (MQ=255)
           aCGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACgg                                                                              >  2:2621609/1‑93 (MQ=255)
           aCGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACgg                                                                              >  1:5703947/1‑93 (MQ=255)
              cTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGa                                                                           <  2:651361/93‑1 (MQ=255)
               tCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGaa                                                                          >  1:8606603/1‑93 (MQ=255)
                cGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAAc                                                                         <  1:548031/93‑1 (MQ=255)
                 ggTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAAct                                                                        >  1:1644488/1‑93 (MQ=255)
                 ggTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAAct                                                                        >  2:2561966/1‑93 (MQ=255)
                   tCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAActtc                                                                      <  2:7592057/93‑1 (MQ=255)
                     aGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAActtctt                                                                    >  2:2984169/1‑93 (MQ=255)
                     aGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAActtctt                                                                    <  2:7044232/93‑1 (MQ=255)
                      gTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCTCACGGCGAACttcttc                                                                   <  2:9559756/93‑1 (MQ=255)
                      gTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACttcttc                                                                   <  2:3183758/93‑1 (MQ=255)
                      gTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACttcttc                                                                   >  2:886142/1‑93 (MQ=255)
                       ttCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTtcttct                                                                  >  2:513665/1‑93 (MQ=255)
                       ttCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTtcttct                                                                  >  2:4426103/1‑93 (MQ=255)
                         cTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGc                                                                >  2:3786720/1‑93 (MQ=255)
                          tttCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGcc                                                               <  1:3406547/93‑1 (MQ=255)
                          tttCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGcc                                                               <  2:4477868/93‑1 (MQ=255)
                           ttCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCt                                                              <  1:373221/93‑1 (MQ=255)
                           ttCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCt                                                              <  1:8873418/93‑1 (MQ=255)
                             cccTTCGCGCAGCGGCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTgg                                                            <  2:1529670/91‑1 (MQ=255)
                              aCTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGc                                                           <  1:3880800/93‑1 (MQ=255)
                               cTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCATATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCg                                                          >  2:5923969/1‑93 (MQ=255)
                                ttCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGt                                                         <  1:9807351/93‑1 (MQ=255)
                                 tCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGtt                                                        <  2:9541234/93‑1 (MQ=255)
                                  cgcgCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTc                                                       <  1:2147947/93‑1 (MQ=255)
                                    cgcAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCaa                                                     <  1:6386984/93‑1 (MQ=255)
                                    cgcAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCaa                                                     <  1:6728688/93‑1 (MQ=255)
                                     gcAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAAt                                                    >  1:241777/1‑93 (MQ=255)
                                      cAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATg                                                   >  1:8678858/1‑93 (MQ=255)
                                        gCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCACTGGa                                                 >  2:1437925/1‑93 (MQ=255)
                                        gCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGa                                                 >  2:9356032/1‑93 (MQ=255)
                                         cgtcgtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGAc                                                <  1:812612/93‑1 (MQ=255)
                                          gtcgtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACa                                               <  2:20625/93‑1 (MQ=255)
                                          gtcgtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACa                                               <  1:324139/93‑1 (MQ=255)
                                          gtcgtGGGTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACa                                               <  1:6874228/93‑1 (MQ=255)
                                           tcgtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACAt                                              >  2:3799954/1‑93 (MQ=255)
                                           tcgtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACAt                                              >  1:2934469/1‑93 (MQ=255)
                                            cgtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATg                                             <  2:424537/93‑1 (MQ=255)
                                            cgtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATg                                             <  2:1409312/93‑1 (MQ=255)
                                             gtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGt                                            <  2:891538/93‑1 (MQ=255)
                                             gtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGt                                            >  2:7975253/1‑93 (MQ=255)
                                             gtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGt                                            <  2:7214003/93‑1 (MQ=255)
                                             gtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGt                                            >  2:1253726/1‑93 (MQ=255)
                                               ggTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGttt                                          >  2:4579050/1‑93 (MQ=255)
                                                gTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGtttt                                         <  2:9344104/93‑1 (MQ=255)
                                                gTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGtttt                                         >  1:1142307/1‑93 (MQ=255)
                                                gTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGtttt                                         >  1:3635920/1‑93 (MQ=255)
                                                gTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGtttt                                         >  1:3169739/1‑93 (MQ=255)
                                                  tGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTtg                                       <  2:4880422/93‑1 (MQ=255)
                                                   ggCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTtgt                                      <  1:1961438/93‑1 (MQ=255)
                                                      aGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGa                                   <  2:3738847/93‑1 (MQ=255)
                                                       gAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGAt                                  >  1:7980838/1‑93 (MQ=255)
                                                       aaaCAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGAt                                  <  1:3521525/92‑1 (MQ=255)
                                                         aCAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATaa                                <  2:6230858/93‑1 (MQ=255)
                                                         aCAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATaa                                <  1:4803869/93‑1 (MQ=255)
                                                          cAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATaaa                               <  1:10045586/93‑1 (MQ=255)
                                                           aGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATaagg                              >  1:7753039/1‑91 (MQ=255)
                                                           aGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAg                              >  2:7843322/1‑93 (MQ=255)
                                                            gTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGc                             >  1:1242728/1‑93 (MQ=255)
                                                             tCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGcc                            >  1:4259248/1‑93 (MQ=255)
                                                               tGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGa                          >  1:5442266/1‑93 (MQ=255)
                                                               tGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGa                          >  2:5412497/1‑93 (MQ=255)
                                                                gCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGAt                         <  1:8270344/93‑1 (MQ=255)
                                                                 cGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATa                        <  1:4364974/93‑1 (MQ=255)
                                                                  ggATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATAc                       >  2:4343346/1‑93 (MQ=255)
                                                                   gATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATAcc                      >  2:9136013/1‑93 (MQ=255)
                                                                   gATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATAcc                      <  1:753953/93‑1 (MQ=255)
                                                                   gATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATAcc                      >  1:4451321/1‑93 (MQ=255)
                                                                    aTCAGTCACCACACCAGATTCGTGTTTTTTCACACGGCGAACTTCTTCTGACTGGCGTTCAATGTACATGTTTTTGCGGATAAAGCCGATAcc                      >  1:9219610/1‑93 (MQ=255)
                                                                     tCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGc                    >  1:4034120/1‑93 (MQ=255)
                                                                      cAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGcc                   <  1:7420594/93‑1 (MQ=255)
                                                                       aGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCt                  <  2:2403862/93‑1 (MQ=255)
                                                                        gTCACCCCACCAGATTCGTG‑TTTTTCACACGTCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCtt                 >  2:3511563/1‑93 (MQ=255)
                                                                         tCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTc                <  2:5104266/93‑1 (MQ=255)
                                                                         tCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTc                >  1:1712900/1‑93 (MQ=255)
                                                                          caccacaccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTcc               <  1:5660096/93‑1 (MQ=255)
                                                                          caccacaccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTcc               <  1:6237028/93‑1 (MQ=255)
                                                                             cacaccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCTCCTTCCTGa            >  2:8871799/1‑93 (MQ=255)
                                                                             cacaccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGa            <  1:9160956/93‑1 (MQ=255)
                                                                              acaccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAg           >  2:6860708/1‑93 (MQ=255)
                                                                               caccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGc          >  2:8450726/1‑93 (MQ=255)
                                                                               caccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGc          >  2:1928963/1‑93 (MQ=255)
                                                                                 ccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCa        >  1:7203369/1‑93 (MQ=255)
                                                                                  caGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCag       <  1:8773873/93‑1 (MQ=255)
                                                                                   cgATTCGTG‑TTTTTCACACGGCGAGCTTCCTCGGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCaga      <  2:2193993/92‑1 (MQ=255)
                                                                                   aGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCaga      <  1:5562540/93‑1 (MQ=255)
                                                                                   aGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCaga      <  1:6612560/93‑1 (MQ=255)
                                                                                     aTTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGc    <  2:6434416/93‑1 (MQ=255)
                                                                                     aTTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGc    <  1:6049871/93‑1 (MQ=255)
                                                                                     aTTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGc    <  1:6483330/93‑1 (MQ=255)
                                                                                       tCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCac  >  2:6081686/1‑92 (MQ=255)
                                                                                       tCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCaa  <  2:5285882/93‑1 (MQ=255)
                                                                                       tCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCaa  >  2:8125457/1‑93 (MQ=255)
                                                                                            |                                                                                        
GCAAAACCGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGTTTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAA  >  NC_000913/2631735‑2631915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGTTTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAGCCAGGCG  >  NC_000913/2631741‑2631925
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CCGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCAC                                                                                            <  SRR4302540.1903031/94‑1 (MQ=60)
 CGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACA                                                                                           >  SRR4302540.3487196/1‑94 (MQ=60)
 CGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACA                                                                                           >  SRR4302540.2213060/1‑94 (MQ=60)
  GTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACAC                                                                                          >  SRR4302540.2179188/1‑94 (MQ=60)
  GTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACAC                                                                                          >  SRR4302540.7766110/1‑94 (MQ=60)
  GTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACAC                                                                                          <  SRR4302540.7642603/94‑1 (MQ=60)
  GTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACAC                                                                                          <  SRR4302540.604480/94‑1 (MQ=60)
  GTTACGCTCGGTCAGTTCTTTCACTTAGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACAC                                                                                          >  SRR4302540.4983611/1‑94 (MQ=60)
   TTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACG                                                                                         >  SRR4302540.5599235/1‑94 (MQ=60)
    TACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGG                                                                                        >  SRR4302540.5766749/1‑94 (MQ=60)
    TACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGG                                                                                        >  SRR4302540.8830905/1‑94 (MQ=60)
     ACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGC                                                                                       >  SRR4302540.10162544/1‑94 (MQ=60)
     ACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGC                                                                                       <  SRR4302540.8791881/94‑1 (MQ=60)
      CGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCG                                                                                      >  SRR4302540.5147375/1‑94 (MQ=60)
       GCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGA                                                                                     <  SRR4302540.5762489/94‑1 (MQ=60)
        CTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAA                                                                                    >  SRR4302540.8706608/1‑94 (MQ=60)
         TCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAAC                                                                                   >  SRR4302540.5518785/1‑94 (MQ=60)
          CGGTGAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACT                                                                                  <  SRR4302540.4234419/94‑1 (MQ=60)
          CGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACT                                                                                  >  SRR4302540.1662450/1‑94 (MQ=60)
          CGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACT                                                                                  <  SRR4302540.554000/94‑1 (MQ=60)
          CGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACT                                                                                  >  SRR4302540.9523509/1‑94 (MQ=60)
           GGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTT                                                                                 >  SRR4302540.3540437/1‑94 (MQ=60)
           GGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTT                                                                                 >  SRR4302540.2009607/1‑94 (MQ=60)
             TCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCT                                                                               >  SRR4302540.7506680/1‑94 (MQ=60)
               AGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGGACTTCTTC                                                                             >  SRR4302540.5656238/1‑94 (MQ=60)
                ggtcgttcaatgCGCGCAGCGTCGTGGGTGGCAGCACACGCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCT                                                                            <  SRR4302540.3830236/82‑1 (MQ=60)
                GTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCT                                                                            >  SRR4302540.4511663/1‑94 (MQ=60)
                GTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCT                                                                            <  SRR4302540.2817667/94‑1 (MQ=60)
                GTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCT                                                                            <  SRR4302540.3830237/94‑1 (MQ=60)
                GTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCT                                                                            <  SRR4302540.3830235/94‑1 (MQ=60)
                GTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCT                                                                            >  SRR4302540.4511665/1‑94 (MQ=60)
                GTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCT                                                                            <  SRR4302540.4648299/94‑1 (MQ=60)
                  TCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGC                                                                          <  SRR4302540.8142942/94‑1 (MQ=60)
                  TCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGC                                                                          >  SRR4302540.4323359/1‑94 (MQ=60)
                   CTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCC                                                                         >  SRR4302540.5886522/1‑94 (MQ=60)
                    TTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCT                                                                        >  SRR4302540.2276520/1‑94 (MQ=60)
                    TTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCT                                                                        <  SRR4302540.3443666/94‑1 (MQ=60)
                    TTTCACTGCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCT                                                                        <  SRR4302540.5035644/94‑1 (MQ=60)
                     TTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTG                                                                       <  SRR4302540.8976112/94‑1 (MQ=60)
                     TTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTG                                                                       <  SRR4302540.377079/94‑1 (MQ=60)
                      TCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGG                                                                      <  SRR4302540.1195574/94‑1 (MQ=60)
                        ACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCG                                                                    >  SRR4302540.1694689/1‑94 (MQ=60)
                        ACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCG                                                                    <  SRR4302540.3922969/94‑1 (MQ=60)
                          TTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTT                                                                  <  SRR4302540.9922144/94‑1 (MQ=60)
                          TTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTT                                                                  <  SRR4302540.3709702/94‑1 (MQ=60)
                            CGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCA                                                                <  SRR4302540.2171044/94‑1 (MQ=60)
                             GCGCAGCGTCGTGGTTGGCAGAACAGTCGGCGGATCAGTCACCACACCAGATGCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAA                                                               <  SRR4302540.10017146/94‑1 (MQ=60)
                              CGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAAT                                                              <  SRR4302540.6458083/94‑1 (MQ=60)
                              CGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAAT                                                              <  SRR4302540.6802980/94‑1 (MQ=60)
                              CGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAAT                                                              >  SRR4302540.244114/1‑94 (MQ=60)
                               GCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATG                                                             >  SRR4302540.8779651/1‑94 (MQ=60)
                                CAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGG                                                            <  SRR4302540.3432662/94‑1 (MQ=60)
                                CAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGG                                                            <  SRR4302540.3016427/94‑1 (MQ=60)
                                 AGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGA                                                           >  SRR4302540.495296/1‑94 (MQ=60)
                                   CGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACA                                                         <  SRR4302540.821241/94‑1 (MQ=60)
                                    GTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACAT                                                        <  SRR4302540.327387/94‑1 (MQ=60)
                                    GTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACAT                                                        >  SRR4302540.9317107/1‑94 (MQ=60)
                                    GTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACAT                                                        >  SRR4302540.2966670/1‑94 (MQ=60)
                                    GTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACAT                                                        >  SRR4302540.684892/1‑94 (MQ=60)
                                    GTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACAT                                                        <  SRR4302540.7878045/94‑1 (MQ=60)
                                    GTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACAT                                                        >  SRR4302540.6479063/1‑94 (MQ=60)
                                    GTCGTGGGTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACAT                                                        <  SRR4302540.6950415/94‑1 (MQ=60)
                                     TCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATG                                                       >  SRR4302540.8234793/1‑94 (MQ=60)
                                      CGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGGACTTCTTCTGCCTGGCGTTCAATGGACATGT                                                      >  SRR4302540.7547749/1‑94 (MQ=60)
                                      CGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGT                                                      <  SRR4302540.6985807/94‑1 (MQ=60)
                                       GTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTT                                                     >  SRR4302540.8499301/1‑94 (MQ=60)
                                       GTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTT                                                     >  SRR4302540.6374523/1‑94 (MQ=60)
                                       GTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGGTCAATGGACATGTT                                                     >  SRR4302540.9046561/1‑94 (MQ=60)
                                        TGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTT                                                    <  SRR4302540.3153298/94‑1 (MQ=60)
                                         GGTTGGCAGAACAGTCTGCGGATCAGTCACCACGCCAGATTCGTG‑TTTTTCGCACGGCGAACTGCTTCTGCCTGGCGTTCAATGGACATGTTTT                                                   <  SRR4302540.3288239/94‑1 (MQ=60)
                                         GGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTT                                                   >  SRR4302540.3204198/1‑94 (MQ=60)
                                         GGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTT                                                   >  SRR4302540.3675722/1‑94 (MQ=60)
                                         GGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTT                                                   >  SRR4302540.1154246/1‑94 (MQ=60)
                                          GTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTT                                                  >  SRR4302540.3888024/1‑94 (MQ=60)
                                          GTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTT                                                  <  SRR4302540.2482332/94‑1 (MQ=60)
                                           TTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTG                                                 >  SRR4302540.6034314/1‑94 (MQ=60)
                                            TGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGT                                                >  SRR4302540.7138609/1‑94 (MQ=60)
                                             GGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTG                                               <  SRR4302540.1982752/94‑1 (MQ=60)
                                              GCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGG                                              <  SRR4302540.44739/94‑1 (MQ=60)
                                              GCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGG                                              >  SRR4302540.2208577/1‑94 (MQ=60)
                                                AGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGAT                                            >  SRR4302540.8073793/1‑94 (MQ=60)
                                                AGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGAT                                            >  SRR4302540.8402107/1‑94 (MQ=60)
                                                 AAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATA                                           <  SRR4302540.3559907/94‑1 (MQ=60)
                                                  AACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAA                                          <  SRR4302540.2674074/94‑1 (MQ=60)
                                                   ACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAA                                         <  SRR4302540.4855281/94‑1 (MQ=60)
                                                   ACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAA                                         >  SRR4302540.910100/1‑94 (MQ=60)
                                                    CAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAGG                                        >  SRR4302540.7843585/1‑94 (MQ=60)
                                                    CAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAG                                        <  SRR4302540.10162367/94‑1 (MQ=60)
                                                     AGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGC                                       >  SRR4302540.3514754/1‑94 (MQ=60)
                                                     AGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGC                                       <  SRR4302540.1564488/94‑1 (MQ=60)
                                                     AGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGC                                       >  SRR4302540.1255405/1‑94 (MQ=60)
                                                      GTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCC                                      >  SRR4302540.8277298/1‑94 (MQ=60)
                                                      GTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCC                                      >  SRR4302540.4305580/1‑94 (MQ=60)
                                                      GTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCC                                      <  SRR4302540.6015975/94‑1 (MQ=60)
                                                      GTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGCCGTTCAATGGACATGTTTTTGTGGATAAAGCC                                      >  SRR4302540.8277297/1‑94 (MQ=60)
                                                       TCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCACTGGACATGTTTTTGTGGATAAAGCAG                                     >  SRR4302540.3591742/1‑94 (MQ=60)
                                                       TCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCG                                     >  SRR4302540.3591741/1‑94 (MQ=60)
                                                       TCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCG                                     <  SRR4302540.681471/94‑1 (MQ=60)
                                                        tnngnnnannagtcaccncnnnngnntngtgTTTTTCACANGGCGAACTTNTTCNGCCNGGCGTNCAATGGACATGTTTTTGTGGATAAAGCCG                                     <  SRR4302540.1192963/63‑1 (MQ=60)
                                                        CTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGA                                    >  SRR4302540.5502147/1‑94 (MQ=60)
                                                        CTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGA                                    >  SRR4302540.5502148/1‑94 (MQ=60)
                                                        CTGCGGATCAGTCACCACACCAGATTCGTG‑CTTTTCACACGGCGACCTTCTTCTGCCTGGCGTTCAATGGACATGGTTTTGCGGATCAAGCCGA                                    >  SRR4302540.321987/1‑94 (MQ=60)
                                                         TGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGAT                                   >  SRR4302540.6745401/1‑94 (MQ=60)
                                                         TGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGAT                                   <  SRR4302540.645647/94‑1 (MQ=60)
                                                          GCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATA                                  <  SRR4302540.8366437/94‑1 (MQ=60)
                                                          GCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATA                                  <  SRR4302540.4394742/94‑1 (MQ=60)
                                                          GCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATA                                  <  SRR4302540.6081094/94‑1 (MQ=60)
                                                          GCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATA                                  <  SRR4302540.9134184/94‑1 (MQ=60)
                                                          GCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATA                                  <  SRR4302540.1197736/94‑1 (MQ=60)
                                                           CGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATAC                                 <  SRR4302540.6632561/94‑1 (MQ=60)
                                                           CGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATAC                                 >  SRR4302540.6775541/1‑94 (MQ=60)
                                                           CGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATAC                                 >  SRR4302540.6991630/1‑94 (MQ=60)
                                                           CGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATAC                                 <  SRR4302540.5556761/94‑1 (MQ=60)
                                                           CGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATAC                                 <  SRR4302540.4412415/94‑1 (MQ=60)
                                                            GGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACC                                >  SRR4302540.6418762/1‑94 (MQ=60)
                                                            GGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACC                                >  SRR4302540.4499576/1‑94 (MQ=60)
                                                             GATCAGTCACCACACCAGATTCGTGTTTTTTCACACGGCGAACTTCTTCTGACTGGCGTTCAATGTACATGTTTTTGCGGATAAAGCCGATACC                                >  SRR4302540.9330193/1‑94 (MQ=60)
                                                             GATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCG                               <  SRR4302540.761989/94‑1 (MQ=60)
                                                             GATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCG                               <  SRR4302540.1764651/94‑1 (MQ=60)
                                                             GATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCG                               >  SRR4302540.1610687/1‑94 (MQ=60)
                                                              ATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGC                              >  SRR4302540.4077620/1‑94 (MQ=60)
                                                                CAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCT                            <  SRR4302540.4263629/94‑1 (MQ=60)
                                                                CAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCT                            <  SRR4302540.7503246/94‑1 (MQ=60)
                                                                 AGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTT                           >  SRR4302540.8908659/1‑94 (MQ=60)
                                                                 AGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTT                           <  SRR4302540.7638152/94‑1 (MQ=60)
                                                                 AGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTT                           <  SRR4302540.3958930/94‑1 (MQ=60)
                                                                 AGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTT                           >  SRR4302540.8908660/1‑94 (MQ=60)
                                                                  GTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTC                          >  SRR4302540.1731406/1‑94 (MQ=60)
                                                                  GTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTC                          >  SRR4302540.4068107/1‑94 (MQ=60)
                                                                  GTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTC                          >  SRR4302540.1731405/1‑94 (MQ=60)
                                                                   TCACCCCCCCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGCCATGTTTTTGTGGATAAAGCCGATACCGCCTTCC                         <  SRR4302540.4187/94‑1 (MQ=60)
                                                                   TCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCC                         <  SRR4302540.926891/94‑1 (MQ=60)
                                                                    CACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCT                        <  SRR4302540.6306416/94‑1 (MQ=60)
                                                                    CACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCT                        <  SRR4302540.4482937/94‑1 (MQ=60)
                                                                    CACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCT                        <  SRR4302540.5722417/94‑1 (MQ=60)
                                                                    CACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCT                        <  SRR4302540.8127416/94‑1 (MQ=60)
                                                                    CACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCT                        <  SRR4302540.6306415/94‑1 (MQ=60)
                                                                    CACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCT                        <  SRR4302540.6622780/94‑1 (MQ=60)
                                                                      CCACCCCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGA                      <  SRR4302540.8745122/94‑1 (MQ=60)
                                                                      CCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGA                      <  SRR4302540.8745124/94‑1 (MQ=60)
                                                                      CCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGA                      >  SRR4302540.1080024/1‑94 (MQ=60)
                                                                      CCACACCAGATTCGTG‑TCTTTGACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGA                      <  SRR4302540.8745121/94‑1 (MQ=60)
                                                                       CACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAG                     <  SRR4302540.9270904/94‑1 (MQ=60)
                                                                       CACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAG                     <  SRR4302540.5663387/94‑1 (MQ=60)
                                                                        CCACCAGATTCGTG‑TTTTTCACACTGCGAACTTCTTCTTCCTGGCGGTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGC                    <  SRR4302540.5413070/94‑1 (MQ=60)
                                                                        CCACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGC                    <  SRR4302540.8063445/94‑1 (MQ=60)
                                                                        ACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGC                    >  SRR4302540.9493542/1‑94 (MQ=60)
                                                                         CACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCC                   >  SRR4302540.3745448/1‑94 (MQ=60)
                                                                         CACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCC                   >  SRR4302540.1524328/1‑94 (MQ=60)
                                                                         CACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCC                   <  SRR4302540.5760735/94‑1 (MQ=60)
                                                                          ACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCA                  >  SRR4302540.7283676/1‑94 (MQ=60)
                                                                          ACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCA                  >  SRR4302540.6775582/1‑94 (MQ=60)
                                                                           CCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAG                 <  SRR4302540.5700052/94‑1 (MQ=60)
                                                                            CAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGA                <  SRR4302540.8875519/94‑1 (MQ=60)
                                                                             AGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAG               <  SRR4302540.5623796/94‑1 (MQ=60)
                                                                             AGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAG               >  SRR4302540.2132255/1‑94 (MQ=60)
                                                                             AGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAG               <  SRR4302540.6685783/94‑1 (MQ=60)
                                                                             AGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAG               >  SRR4302540.3008688/1‑94 (MQ=60)
                                                                             AGATTCGTG‑TTTTTCACACGGCGAACTTCTGCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAG               <  SRR4302540.2665120/94‑1 (MQ=60)
                                                                              GATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGC              >  SRR4302540.7494875/1‑94 (MQ=60)
                                                                              GATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGC              >  SRR4302540.3266021/1‑94 (MQ=60)
                                                                               ATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCA             <  SRR4302540.6117103/94‑1 (MQ=60)
                                                                               ATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCA             <  SRR4302540.6555442/94‑1 (MQ=60)
                                                                                 TCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAAT           <  SRR4302540.6239029/94‑1 (MQ=60)
                                                                                  CGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATA          <  SRR4302540.4927952/94‑1 (MQ=60)
                                                                                  CGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATA          >  SRR4302540.9140769/1‑94 (MQ=60)
                                                                                   GTG‑TTTTTCACACGGCGCACTTCTTCTGCCTGGCGTTCCATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGACAGAGCAATAC         >  SRR4302540.427465/1‑94 (MQ=60)
                                                                                   GTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAG         >  SRR4302540.4542217/1‑94 (MQ=60)
                                                                                   GTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAG         >  SRR4302540.427466/1‑94 (MQ=60)
                                                                                   GTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAG         >  SRR4302540.427467/1‑94 (MQ=60)
                                                                                       TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAGCCA      >  SRR4302540.3753232/1‑94 (MQ=60)
                                                                                       TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAGCCA      <  SRR4302540.7827940/94‑1 (MQ=60)
                                                                                        TTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAGCCAG     <  SRR4302540.1288378/94‑1 (MQ=60)
                                                                                         TTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAGCCAGG    <  SRR4302540.8062133/94‑1 (MQ=60)
                                                                                         TTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAACCAGG    >  SRR4302540.5048859/1‑94 (MQ=60)
                                                                                           TCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAGCCAGGCG  <  SRR4302540.3936489/94‑1 (MQ=60)
                                                                                           TCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAGCCAGGCG  <  SRR4302540.7005675/94‑1 (MQ=60)
                                                                                           TCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAGCCAGGCG  <  SRR4302540.4583995/94‑1 (MQ=60)
                                                                                           |                                                                                             
CCGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGTTTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAGCCAGGCG  >  NC_000913/2631741‑2631925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑