Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,644,603 (G)6→5 coding (743/2313 nt) pbpC ← putative peptidoglycan enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,644,5980G.99.6% 1062.0 / ‑3.7 236coding (748/2313 nt)pbpCputative peptidoglycan enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base . (110/125);  total (110/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.07e-01

Only 100 of 269 total aligned reads displayed.

AAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTA  >  NC_000913/2644506‑2644686
                                                                                            |                                                                                        
aaGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCg                                                                                          >  2:2866962/1‑93 (MQ=255)
aaGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCg                                                                                          <  2:1032983/93‑1 (MQ=255)
 aGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCgg                                                                                         <  2:8256465/93‑1 (MQ=255)
 aGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCgg                                                                                         <  2:7784055/93‑1 (MQ=255)
  gACCGCCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCggg                                                                                        <  1:1499108/93‑1 (MQ=255)
    ccGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCggggg                                                                                      <  1:3279425/93‑1 (MQ=255)
     cGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCgggggc                                                                                     >  1:4458949/1‑92 (MQ=255)
      ggCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCgggggcc                                                                                    >  1:6095332/1‑91 (MQ=255)
      ggCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCgggggcc                                                                                    <  1:7815265/93‑3 (MQ=255)
       gCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCgggggcca                                                                                   >  1:6933374/1‑90 (MQ=255)
        cATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCgggggccag                                                                                  >  2:2164000/1‑89 (MQ=255)
         aTCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGgccag                                                                                 >  1:4731224/1‑93 (MQ=255)
         aTCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGgccagc                                                                                <  1:130497/93‑1 (MQ=255)
         aTCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGgccagc                                                                                >  1:2009732/1‑93 (MQ=255)
          tCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGgccagcc                                                                               <  2:9281683/93‑1 (MQ=255)
          tCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGCTGCGGCATTTGTC‑GGGGgccagcc                                                                               >  1:2416919/1‑93 (MQ=255)
           ccAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGgccagcca                                                                              >  1:7414196/1‑93 (MQ=255)
           ccAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGgccagcca                                                                              <  2:4503436/93‑1 (MQ=255)
            cAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGccagccag                                                                             >  1:7605684/1‑93 (MQ=255)
             aaCGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGTTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGa                                                                            <  1:3754220/93‑1 (MQ=255)
              aCGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCGGCCAGAt                                                                           >  1:2730123/1‑93 (MQ=255)
              aCGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGAt                                                                           <  1:70249/93‑1 (MQ=255)
              aCGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGAt                                                                           >  1:937434/1‑93 (MQ=255)
                 tagtagTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATggg                                                                        <  2:6656215/93‑1 (MQ=255)
                 tagtagTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATggg                                                                        >  2:4900630/1‑93 (MQ=255)
                 tagtagTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATggg                                                                        <  1:9524150/93‑1 (MQ=255)
                 tagtagTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATggg                                                                        >  1:9826639/1‑93 (MQ=255)
                  agtagtGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGt                                                                       <  2:544631/93‑1 (MQ=255)
                   gtagtGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGtt                                                                      >  1:4313723/1‑93 (MQ=255)
                    tagtGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGttc                                                                     >  2:1855675/1‑93 (MQ=255)
                     agtGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGttct                                                                    <  2:7876261/93‑1 (MQ=255)
                      gtGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGttctt                                                                   >  2:5588340/1‑93 (MQ=255)
                       tGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGttcttc                                                                  >  2:2527701/1‑93 (MQ=255)
                       tGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGttcttc                                                                  >  2:7671355/1‑93 (MQ=255)
                        gATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTcc                                                                 >  2:2311706/1‑93 (MQ=255)
                         aTTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTccc                                                                >  2:4148328/1‑93 (MQ=255)
                            ttGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTg                                                             >  1:4989848/1‑93 (MQ=255)
                             tGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGa                                                            <  1:2233463/93‑1 (MQ=255)
                             tGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGa                                                            <  2:9383381/93‑1 (MQ=255)
                              gTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGAc                                                           <  1:6057782/93‑1 (MQ=255)
                              gTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGAc                                                           <  1:5410041/93‑1 (MQ=255)
                                cGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGActc                                                         <  2:9176178/93‑1 (MQ=255)
                                cGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGActc                                                         >  2:5406825/1‑93 (MQ=255)
                                 gcttttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCTAGCCAGATGGGTTCTTCCCTTGActct                                                        <  1:1323237/93‑1 (MQ=255)
                                 gcttttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGActct                                                        >  1:2509832/1‑93 (MQ=255)
                                 gcttttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGActct                                                        >  2:6986533/1‑93 (MQ=255)
                                  cttttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCtt                                                       <  1:6335695/93‑1 (MQ=255)
                                   ttttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCttt                                                      <  1:7292328/93‑1 (MQ=255)
                                    tttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCtttt                                                     >  2:8076473/1‑93 (MQ=255)
                                    tttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCtttt                                                     >  1:9113964/1‑93 (MQ=255)
                                     ttgcttttACCGAGCATCATGCGCGAAAGCAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGTCCAGCCAGATGGGTTCTTCCCTTGACCCTTTTa                                                    <  2:1485421/93‑1 (MQ=255)
                                     ttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTa                                                    <  2:6043936/93‑1 (MQ=255)
                                     ttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTa                                                    >  1:6731902/1‑93 (MQ=255)
                                      tgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTAc                                                   <  2:3963369/93‑1 (MQ=255)
                                      tgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTCAc                                                   >  1:5090150/1‑93 (MQ=255)
                                        cttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCt                                                 >  1:9055315/1‑93 (MQ=255)
                                            tACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTc                                             >  2:1593723/1‑93 (MQ=255)
                                                gAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACgg                                         >  2:6823615/1‑93 (MQ=255)
                                                gAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACgg                                         <  2:5932165/93‑1 (MQ=255)
                                                 aGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACggg                                        >  1:5695897/1‑93 (MQ=255)
                                                  gCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGa                                       >  2:612333/1‑93 (MQ=255)
                                                   catcatGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGa                                       <  2:9723640/93‑1 (MQ=255)
                                                    atcatGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGAcc                                     <  1:9681067/93‑1 (MQ=255)
                                                    atcatGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGGTCTTCCCTTGACTCTTTTACCTGCTCACGGGAcc                                     >  2:9222917/1‑93 (MQ=255)
                                                     tcatGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTTCTCACGGAACca                                    >  1:1935297/1‑93 (MQ=255)
                                                     tcatGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACca                                    >  2:119642/1‑93 (MQ=255)
                                                     tcatGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACca                                    >  2:7143251/1‑93 (MQ=255)
                                                     tcatGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACca                                    >  2:5904803/1‑93 (MQ=255)
                                                       atGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACcaca                                  <  1:5893612/93‑1 (MQ=255)
                                                        tGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACcacac                                 <  1:4015239/93‑1 (MQ=255)
                                                         gcgcGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACcacaca                                <  2:3360435/93‑1 (MQ=255)
                                                         gcgcGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACcacaca                                <  2:3492124/93‑1 (MQ=255)
                                                         gcgcGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAATTGGGATCTTCCCTTTACTCTTTTACCTGCTCACGGGACcacaca                                >  2:8653321/1‑93 (MQ=255)
                                                           gcgAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACAcc                              <  1:2970433/93‑1 (MQ=255)
                                                           gcgAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACAcc                              <  1:171712/93‑1 (MQ=255)
                                                             gAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCtt                            <  1:4518518/93‑1 (MQ=255)
                                                             gAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCtt                            >  2:4326564/1‑93 (MQ=255)
                                                               acaCAGCGGTGCGAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGc                          <  1:8826640/91‑1 (MQ=255)
                                                                aaCAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCa                         <  1:4044773/93‑1 (MQ=255)
                                                                  cAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACg                       <  1:3578638/93‑1 (MQ=255)
                                                                    gCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGc                     <  1:212090/93‑1 (MQ=255)
                                                                     cGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGcc                    <  1:5794392/93‑1 (MQ=255)
                                                                      ggTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGATTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCa                   <  1:5334322/93‑1 (MQ=255)
                                                                       gTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCAt                  >  1:8283315/1‑93 (MQ=255)
                                                                        tGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATc                 >  1:6254601/1‑93 (MQ=255)
                                                                         gCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATcc                <  1:8530202/93‑1 (MQ=255)
                                                                         gCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATcc                <  2:4610149/93‑1 (MQ=255)
                                                                           cAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGt              >  2:1328746/1‑93 (MQ=255)
                                                                           cAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGt              <  1:5632704/93‑1 (MQ=255)
                                                                           cAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGt              <  2:7306058/93‑1 (MQ=255)
                                                                           cAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGt              <  2:6607333/93‑1 (MQ=255)
                                                                            aGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGtt             <  2:1555275/93‑1 (MQ=255)
                                                                            aGTTGCGGCATGTGGCGGGGGG‑AAGCAAGATGGGTTCTTCCCTTGACTCTTTTGCCTGCTCACGGGACCACACACCTTGCGCGGCCATCCGtt             <  1:3470388/93‑1 (MQ=255)
                                                                             gTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCCTCCGTTc            >  1:9001857/1‑93 (MQ=255)
                                                                             gTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTc            <  1:3836727/93‑1 (MQ=255)
                                                                              ttGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCg           >  2:6517709/1‑93 (MQ=255)
                                                                                   gCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGAACCACACACCTTGCACGGCCATCCGTTCGAGCAc      <  2:6372062/93‑1 (MQ=255)
                                                                                     aTTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACtt    >  2:5026801/1‑93 (MQ=255)
                                                                                      tttGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACttt   >  1:10026986/1‑93 (MQ=255)
                                                                                      tttGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACttt   <  1:4390618/93‑1 (MQ=255)
                                                                                            |                                                                                        
AAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTA  >  NC_000913/2644506‑2644686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCG  >  NC_000913/2644510‑2644695
                                                                                             |                                                                                            
CCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGC                                                                                              >  SRR4302540.4507284/1‑94 (MQ=60)
CCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGC                                                                                              <  SRR4302540.3315245/94‑1 (MQ=60)
 CGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGCC                                                                                             >  SRR4302540.5436654/1‑94 (MQ=60)
 CGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGCC                                                                                             >  SRR4302540.6163175/1‑94 (MQ=60)
 CGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGCC                                                                                             >  SRR4302540.2931689/1‑94 (MQ=60)
  GGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCA                                                                                           >  SRR4302540.7010533/1‑94 (MQ=60)
  GGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCA                                                                                           <  SRR4302540.7906596/94‑1 (MQ=60)
    CATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAG                                                                                          >  SRR4302540.4781748/1‑94 (MQ=60)
    CATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGC                                                                                         >  SRR4302540.2031466/1‑94 (MQ=60)
     ATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCC                                                                                        <  SRR4302540.131870/94‑1 (MQ=60)
     ATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGCTGCGGCATTTGTC‑GGGGGCCAGCC                                                                                        >  SRR4302540.2442963/1‑94 (MQ=60)
      TCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCA                                                                                       >  SRR4302540.7496779/1‑94 (MQ=60)
       CCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAG                                                                                      >  SRR4302540.8840239/1‑94 (MQ=60)
       CCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAG                                                                                      >  SRR4302540.7691149/1‑94 (MQ=60)
        CAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGA                                                                                     >  SRR4302540.1100384/1‑94 (MQ=60)
         AACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGTTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGAT                                                                                    <  SRR4302540.3795176/94‑1 (MQ=60)
         AACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCGGCCAGAT                                                                                    >  SRR4302540.2759580/1‑94 (MQ=60)
         AACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGAT                                                                                    >  SRR4302540.947252/1‑94 (MQ=60)
         AACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGAT                                                                                    <  SRR4302540.6255661/94‑1 (MQ=60)
          ACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATG                                                                                   <  SRR4302540.9713779/94‑1 (MQ=60)
          ACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATG                                                                                   <  SRR4302540.70961/94‑1 (MQ=60)
           CGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGG                                                                                  <  SRR4302540.9502909/94‑1 (MQ=60)
           CGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGG                                                                                  >  SRR4302540.4191369/1‑94 (MQ=60)
           CGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGG                                                                                  <  SRR4302540.9269375/94‑1 (MQ=60)
            GTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGG                                                                                 >  SRR4302540.9941591/1‑94 (MQ=60)
            GTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGG                                                                                 >  SRR4302540.5915061/1‑94 (MQ=60)
             TAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGT                                                                                <  SRR4302540.9636921/94‑1 (MQ=60)
              AGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTT                                                                               >  SRR4302540.4360688/1‑94 (MQ=60)
              AGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTT                                                                               >  SRR4302540.1604978/1‑94 (MQ=60)
               GTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAACCAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTC                                                                              <  SRR4302540.2424615/94‑1 (MQ=60)
                TAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCT                                                                             <  SRR4302540.1414340/94‑1 (MQ=60)
                TAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCT                                                                             <  SRR4302540.3643829/94‑1 (MQ=60)
                 AGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTT                                                                            <  SRR4302540.4943608/94‑1 (MQ=60)
                 AGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTT                                                                            <  SRR4302540.9132164/94‑1 (MQ=60)
                  GTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTC                                                                           <  SRR4302540.1876028/94‑1 (MQ=60)
                  GTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTC                                                                           >  SRR4302540.1969917/1‑94 (MQ=60)
                  GTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTC                                                                           >  SRR4302540.3403791/1‑94 (MQ=60)
                   TGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCC                                                                          >  SRR4302540.6054113/1‑94 (MQ=60)
                   TGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCC                                                                          >  SRR4302540.3553024/1‑94 (MQ=60)
                    GATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAATAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCC                                                                         >  SRR4302540.1852663/1‑94 (MQ=60)
                    GATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCC                                                                         >  SRR4302540.2401941/1‑94 (MQ=60)
                    GATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCC                                                                         <  SRR4302540.7694427/94‑1 (MQ=60)
                    GATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCC                                                                         >  SRR4302540.9511808/1‑94 (MQ=60)
                     ATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCT                                                                        >  SRR4302540.5713126/1‑94 (MQ=60)
                     ATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCT                                                                        >  SRR4302540.5819217/1‑94 (MQ=60)
                     ATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCT                                                                        <  SRR4302540.5933732/94‑1 (MQ=60)
                       TTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTG                                                                      >  SRR4302540.5043875/1‑94 (MQ=60)
                       TTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTG                                                                      <  SRR4302540.2718823/94‑1 (MQ=60)
                         TGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGAC                                                                    <  SRR4302540.2257500/94‑1 (MQ=60)
                          GTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACT                                                                   <  SRR4302540.5469526/94‑1 (MQ=60)
                          GTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACT                                                                   <  SRR4302540.6125111/94‑1 (MQ=60)
                            CGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCT                                                                 >  SRR4302540.2536936/1‑94 (MQ=60)
                             tgttgtgccTTTACCGAGCATCATGCGCCAAAACAGCGGTGCCAGTTGCCGCATTGGGC‑GGGTGCTAGCCAGATGGGTTCTTCCCTTGGCTCTT                                                                <  SRR4302540.1337033/85‑1 (MQ=43)
                             TCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTT                                                                <  SRR4302540.1337031/94‑1 (MQ=60)
                             GCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCTAGCCAGATGGGTTCTTCCCTTGACTCTT                                                                <  SRR4302540.1337032/94‑1 (MQ=60)
                              CTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTT                                                               <  SRR4302540.6406243/94‑1 (MQ=60)
                               TTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTT                                                              <  SRR4302540.7373710/94‑1 (MQ=60)
                               TTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTT                                                              >  SRR4302540.9223503/1‑94 (MQ=60)
                                TTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTA                                                             >  SRR4302540.6409332/1‑94 (MQ=60)
                                TTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTA                                                             >  SRR4302540.6806242/1‑94 (MQ=60)
                                TTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCAGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTA                                                             <  SRR4302540.405865/94‑1 (MQ=60)
                                 TTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTCAC                                                            >  SRR4302540.5145197/1‑94 (MQ=60)
                                   GCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCT                                                          >  SRR4302540.9164023/1‑94 (MQ=60)
                                    CTTTTACCTAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTG                                                         >  SRR4302540.7334660/1‑94 (MQ=60)
                                      TTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCT                                                       <  SRR4302540.1515181/94‑1 (MQ=60)
                                       TTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTC                                                      >  SRR4302540.3526626/1‑94 (MQ=60)
                                        TACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCA                                                     >  SRR4302540.9180253/1‑94 (MQ=60)
                                          CCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACG                                                   <  SRR4302540.9359550/94‑1 (MQ=60)
                                           CGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGG                                                  <  SRR4302540.5806279/94‑1 (MQ=60)
                                            GAGCATCATGCGGGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGG                                                 <  SRR4302540.2113044/94‑1 (MQ=60)
                                            GAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGG                                                 >  SRR4302540.5758610/1‑94 (MQ=60)
                                            GAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGG                                                 <  SRR4302540.7357816/94‑1 (MQ=60)
                                            GAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGG                                                 >  SRR4302540.4088340/1‑94 (MQ=60)
                                             AGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGA                                                >  SRR4302540.6157387/1‑94 (MQ=60)
                                             AGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGA                                                >  SRR4302540.6157386/1‑94 (MQ=60)
                                              GCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGAC                                               <  SRR4302540.8950450/94‑1 (MQ=60)
                                              GCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGAC                                               <  SRR4302540.4366256/94‑1 (MQ=60)
                                               CATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACC                                              <  SRR4302540.6538299/94‑1 (MQ=60)
                                               CATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACC                                              >  SRR4302540.8110802/1‑94 (MQ=60)
                                                ATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCTTTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCA                                             <  SRR4302540.9794974/94‑1 (MQ=60)
                                                ATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTTCTCACGGAACCA                                             >  SRR4302540.1956382/1‑94 (MQ=60)
                                                ATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCC                                             >  SRR4302540.4361588/1‑94 (MQ=60)
                                                ATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCA                                             >  SRR4302540.4927405/1‑94 (MQ=60)
                                                ATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCA                                             >  SRR4302540.8603844/1‑94 (MQ=60)
                                                ATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCA                                             <  SRR4302540.9794973/94‑1 (MQ=60)
                                                  CATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACA                                           <  SRR4302540.4731552/94‑1 (MQ=60)
                                                   ATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACAC                                          <  SRR4302540.5065834/94‑1 (MQ=60)
                                                   ATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACAC                                          <  SRR4302540.5958709/94‑1 (MQ=60)
                                                    TGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACA                                         <  SRR4302540.4058490/94‑1 (MQ=60)
                                                     GCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACAC                                        <  SRR4302540.3830998/94‑1 (MQ=60)
                                                       GCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCT                                      <  SRR4302540.3003238/94‑1 (MQ=60)
                                                       GCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCT                                      <  SRR4302540.173430/94‑1 (MQ=60)
                                                         GAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTG                                    >  SRR4302540.8503899/1‑94 (MQ=60)
                                                         GAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTG                                    <  SRR4302540.4567458/94‑1 (MQ=60)
                                                          AAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGCCTCTTTTACCTGCTCACGGGACCACACACCTTGC                                   <  SRR4302540.2724437/94‑1 (MQ=60)
                                                           ACACAGCGGTGCGAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCA                                  <  SRR4302540.8928714/94‑1 (MQ=60)
                                                            AGCAGCGGTGCGAGTTGCGGCATGTGTC‑GGGGGCCCGCCAGATGGGTTCTTCCCTGGAGTCTTTTACCTGCTCACGGGACCACACACCTTGCAC                                 <  SRR4302540.1021499/94‑1 (MQ=60)
                                                            AACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCAC                                 <  SRR4302540.4088380/94‑1 (MQ=60)
                                                            AACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCAC                                 <  SRR4302540.7027139/94‑1 (MQ=60)
                                                            AACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCAC                                 <  SRR4302540.3356655/94‑1 (MQ=60)
                                                            AACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCAC                                 <  SRR4302540.9161601/94‑1 (MQ=60)
                                                             ACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACG                                <  SRR4302540.63907/94‑1 (MQ=60)
                                                              CAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGG                               <  SRR4302540.3617727/94‑1 (MQ=60)
                                                                GCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCC                             <  SRR4302540.214170/94‑1 (MQ=60)
                                                                GCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCC                             >  SRR4302540.445007/1‑94 (MQ=60)
                                                                 CGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCA                            >  SRR4302540.4445542/1‑94 (MQ=60)
                                                                 CGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCA                            <  SRR4302540.467511/94‑1 (MQ=60)
                                                                 CGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCA                            <  SRR4302540.2449912/94‑1 (MQ=60)
                                                                 CGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCA                            <  SRR4302540.5858421/94‑1 (MQ=60)
                                                                  GGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGATTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCAT                           <  SRR4302540.5392783/94‑1 (MQ=60)
                                                                  GGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCAT                           >  SRR4302540.8379525/1‑94 (MQ=60)
                                                                   ggttgcagttggggcatttggcGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCAT                           <  SRR4302540.3325231/72‑1 (MQ=60)
                                                                   GTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATC                          <  SRR4302540.5148507/94‑1 (MQ=60)
                                                                   GTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATC                          >  SRR4302540.6324191/1‑94 (MQ=60)
                                                                    TGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCC                         <  SRR4302540.2180675/94‑1 (MQ=60)
                                                                     GCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCG                        >  SRR4302540.5575367/1‑94 (MQ=60)
                                                                     GCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCG                        <  SRR4302540.8629314/94‑1 (MQ=60)
                                                                     GCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCG                        >  SRR4302540.5575369/1‑94 (MQ=60)
                                                                      CCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTATTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGT                       >  SRR4302540.2021688/1‑94 (MQ=60)
                                                                       CAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTT                      <  SRR4302540.5694699/94‑1 (MQ=60)
                                                                        AGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCCTCCGTTC                     >  SRR4302540.9109858/1‑94 (MQ=60)
                                                                        AGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTC                     <  SRR4302540.7409456/94‑1 (MQ=60)
                                                                        AGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTC                     <  SRR4302540.4531811/94‑1 (MQ=60)
                                                                        AGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTC                     <  SRR4302540.6892457/94‑1 (MQ=60)
                                                                        AGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTC                     >  SRR4302540.9933751/1‑94 (MQ=60)
                                                                        AGTTGCGGCATGTGGCGGGGGG‑AAGCAAGATGGGTTCTTCCCTTGACTCTTTTGCCTGCTCACGGGACCACACACCTTGCGCGGCCATCCGTTC                     <  SRR4302540.3508169/94‑1 (MQ=60)
                                                                         GTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCG                    <  SRR4302540.3878462/94‑1 (MQ=60)
                                                                         GTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGCGACCACACACCTTGCACGGCCATCCGTTCG                    >  SRR4302540.7750573/1‑94 (MQ=60)
                                                                               GCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACT              >  SRR4302540.3261556/1‑94 (MQ=60)
                                                                               GCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACT              <  SRR4302540.10040208/94‑1 (MQ=60)
                                                                                CATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTT             >  SRR4302540.3620705/1‑94 (MQ=60)
                                                                                CATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTT             <  SRR4302540.2202455/94‑1 (MQ=60)
                                                                                CATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTT             <  SRR4302540.2907483/94‑1 (MQ=60)
                                                                                 ATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTT            >  SRR4302540.10143652/1‑94 (MQ=60)
                                                                                 ATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTT            <  SRR4302540.8244553/94‑1 (MQ=60)
                                                                                 ATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTT            >  SRR4302540.4023646/1‑94 (MQ=60)
                                                                                 ATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTT            <  SRR4302540.5466918/94‑1 (MQ=60)
                                                                                  TTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTA           <  SRR4302540.4438264/94‑1 (MQ=60)
                                                                                  TTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTA           >  SRR4302540.530952/1‑94 (MQ=60)
                                                                                   TTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTAT          <  SRR4302540.7535898/94‑1 (MQ=60)
                                                                                    TGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATT         <  SRR4302540.3832422/94‑1 (MQ=60)
                                                                                    TGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATT         >  SRR4302540.917928/1‑94 (MQ=60)
                                                                                    TGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATT         <  SRR4302540.7081084/94‑1 (MQ=60)
                                                                                       tcGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTAC       >  SRR4302540.1076723/3‑94 (MQ=60)
                                                                                       tcGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTAC       <  SRR4302540.5105573/92‑1 (MQ=60)
                                                                                        CGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACG      <  SRR4302540.4702899/94‑1 (MQ=60)
                                                                                        CGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACG      <  SRR4302540.4702898/94‑1 (MQ=60)
                                                                                        CGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACG      <  SRR4302540.7530369/94‑1 (MQ=60)
                                                                                        CGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACG      <  SRR4302540.7530370/94‑1 (MQ=60)
                                                                                        CGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACG      <  SRR4302540.2794386/94‑1 (MQ=60)
                                                                                           GGGCCAGCCAGATGGGTTCTTCTCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGC   <  SRR4302540.9973124/94‑1 (MQ=60)
                                                                                           GGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGC   >  SRR4302540.8337050/1‑94 (MQ=60)
                                                                                            GGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCG  <  SRR4302540.8803841/94‑1 (MQ=60)
                                                                                             |                                                                                            
CCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCG  >  NC_000913/2644510‑2644695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑