Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,644,603 (G)6→5 coding (743/2313 nt) pbpC ← putative peptidoglycan enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,644,5980G.100.0% 935.4 / NA 203coding (748/2313 nt)pbpCputative peptidoglycan enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (109/94);  total (109/94)

Only 100 of 224 total aligned reads displayed.

AAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTA  >  NC_000913/2644506‑2644686
                                                                                            |                                                                                        
aaGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCg                                                                                          <  2:2776192/93‑1 (MQ=255)
aaGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCg                                                                                          <  1:2376670/93‑1 (MQ=255)
 aGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCgg                                                                                         <  2:2175110/93‑1 (MQ=255)
   aCCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCgggg                                                                                       >  2:4766000/1‑93 (MQ=255)
   aCCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCgggg                                                                                       >  2:343607/1‑93 (MQ=255)
       gCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCgggggcca                                                                                   >  1:4623551/1‑90 (MQ=255)
         aTCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGgccagc                                                                                >  2:1535278/1‑93 (MQ=255)
         aTCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGgccagc                                                                                >  1:1724411/1‑93 (MQ=255)
         aTCCAACGTAGGAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGgccagc                                                                                >  2:5848461/1‑93 (MQ=255)
             aaCGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGa                                                                            >  2:4285980/1‑93 (MQ=255)
             aaCGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGa                                                                            <  2:4100062/93‑1 (MQ=255)
             aaCGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCAGTTGTC‑GGGGGCCAGCCAGa                                                                            <  2:6145915/93‑1 (MQ=255)
              aCGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGAt                                                                           >  2:3989420/1‑93 (MQ=255)
              aCGTAGTAGTGATATTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGAt                                                                           <  1:3602831/93‑1 (MQ=255)
                gtagtaGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATgg                                                                         >  1:4342546/1‑93 (MQ=255)
                gtagtaGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATgg                                                                         <  1:5180858/93‑1 (MQ=255)
                gtagtaGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATgg                                                                         <  1:4738768/93‑1 (MQ=255)
                 tagtagTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATggg                                                                        <  1:3695456/93‑1 (MQ=255)
                 tagtagTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATggg                                                                        <  1:4759689/93‑1 (MQ=255)
                 tagtagTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATggg                                                                        >  2:5378412/1‑93 (MQ=255)
                 tagtagTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATggg                                                                        >  1:1051925/1‑93 (MQ=255)
                   gtagtGATTTTATCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGtt                                                                      <  1:3336315/93‑1 (MQ=255)
                      gtGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGttctt                                                                   >  2:1461406/1‑93 (MQ=255)
                       tGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGttcttc                                                                  <  2:2044666/93‑1 (MQ=255)
                       tGATTTTGTCGCTTTTGCTTTTACCGAGAATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGttcttc                                                                  >  1:1283150/1‑93 (MQ=255)
                          ttttGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCt                                                               >  1:1564076/1‑93 (MQ=255)
                          ttttGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCt                                                               <  1:2000671/93‑1 (MQ=255)
                            ttGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTg                                                             <  1:2320243/93‑1 (MQ=255)
                            ttGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTg                                                             >  2:1153677/1‑93 (MQ=255)
                             tGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGa                                                            <  1:1609544/93‑1 (MQ=255)
                              gTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGAc                                                           >  2:5082606/1‑93 (MQ=255)
                                  cttttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCtt                                                       <  1:3702627/93‑1 (MQ=255)
                                  cttttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCtt                                                       >  2:1007242/1‑93 (MQ=255)
                                   ttttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCttt                                                      >  2:2436565/1‑93 (MQ=255)
                                   ttttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCttt                                                      >  1:5740774/1‑93 (MQ=255)
                                    tttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCtttt                                                     <  1:4205523/93‑1 (MQ=255)
                                    tttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCtttt                                                     <  2:1084539/93‑1 (MQ=255)
                                     ttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTa                                                    >  2:1744005/1‑93 (MQ=255)
                                     ttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTa                                                    <  1:385441/93‑1 (MQ=255)
                                     ttgcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTa                                                    >  1:3158880/1‑93 (MQ=255)
                                       tcttttACCGAGCATCATGCGCGAAACCAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGCCTCTTTTAcc                                                  <  1:1115084/92‑1 (MQ=255)
                                       gcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTAcc                                                  >  2:2552463/1‑93 (MQ=255)
                                       gcttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTAcc                                                  >  2:2049865/1‑93 (MQ=255)
                                        cttttACCGAGCATCATGCGCGAACACAACGGAGCCAATTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCt                                                 <  2:6304528/93‑1 (MQ=255)
                                        cttttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCt                                                 <  2:1879947/93‑1 (MQ=255)
                                          tttACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGc                                               <  1:4078441/93‑1 (MQ=255)
                                           ttACCGAGCATCATGCGCGAAAACAGTGGTGCCAGTTGCGCCATTTG‑CCGGGTGACAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCt                                              <  2:1820258/93‑1 (MQ=255)
                                            tACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTc                                             <  2:3591231/93‑1 (MQ=255)
                                             aCCGAGCATCATGCGCGAAAACCGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCa                                            <  2:3426986/93‑1 (MQ=255)
                                             aCCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCa                                            >  1:4157948/1‑93 (MQ=255)
                                             aCCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCa                                            <  2:3060331/93‑1 (MQ=255)
                                               cGAGCATCATGCGCTAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGTTTCTTCCCTTGACTCTTTTACCTGCTCACg                                          <  2:3739431/93‑1 (MQ=255)
                                               cGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACg                                          >  2:5065383/1‑93 (MQ=255)
                                               cGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACg                                          >  2:6287501/1‑93 (MQ=255)
                                                gAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACgg                                         >  2:3897546/1‑93 (MQ=255)
                                                gAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACgg                                         >  1:6047923/1‑93 (MQ=255)
                                                gAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCGGCTCACgg                                         <  2:6129373/93‑1 (MQ=255)
                                                 aGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACggg                                        >  1:2742865/1‑93 (MQ=255)
                                                  gCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGa                                       <  1:5238455/93‑1 (MQ=255)
                                                   catcatGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGAc                                      >  1:1812352/1‑93 (MQ=255)
                                                    atcatGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGAcc                                     >  1:5350839/1‑93 (MQ=255)
                                                    atcatGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGAcc                                     <  2:1655472/93‑1 (MQ=255)
                                                     tcatGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACca                                    >  2:3595862/1‑93 (MQ=255)
                                                     tcatGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACca                                    >  2:562571/1‑93 (MQ=255)
                                                     tcatGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACca                                    <  2:3359527/93‑1 (MQ=255)
                                                      catGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACcac                                   <  1:2481120/93‑1 (MQ=255)
                                                      catGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACcac                                   <  1:2125934/93‑1 (MQ=255)
                                                       atGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACcaca                                  >  2:211533/1‑93 (MQ=255)
                                                        tGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACcacac                                 <  2:5330749/93‑1 (MQ=255)
                                                        tGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACcacac                                 >  2:2919555/1‑93 (MQ=255)
                                                         gcgcGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACcacaca                                >  2:625515/1‑93 (MQ=255)
                                                           gcgAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACAcc                              >  2:5123682/1‑93 (MQ=255)
                                                            cgAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCt                             <  1:1004190/93‑1 (MQ=255)
                                                             gAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCtt                            >  1:4437385/1‑93 (MQ=255)
                                                              aaaaCAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGCCCTTTTTACTTGCTCCCGGACCCACACCCCttc                           >  1:2672260/1‑92 (MQ=255)
                                                               aagcAGCGGTGCCAGTTGCGGCATGTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGc                          <  2:4173683/90‑1 (MQ=255)
                                                               aaaCAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGc                          <  2:16961/93‑1 (MQ=255)
                                                                 aCAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCAc                        >  2:5528089/1‑93 (MQ=255)
                                                                 aCAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCAc                        <  2:4534786/93‑1 (MQ=255)
                                                                     cGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGcc                    <  1:4506790/93‑1 (MQ=255)
                                                                     cGGTGCCAGTTGCGACATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGcc                    <  1:5474576/93‑1 (MQ=255)
                                                                       gTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCAt                  >  2:4349928/1‑93 (MQ=255)
                                                                        tGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATc                 <  1:5497767/93‑1 (MQ=255)
                                                                         gCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTAACGGGACCACACACCTTGCACGGCCATcc                >  2:6187689/1‑93 (MQ=255)
                                                                          ccAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCg               <  2:4118863/93‑1 (MQ=255)
                                                                            aGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGtt             <  1:5017918/93‑1 (MQ=255)
                                                                             gTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTCCCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTc            <  2:1984537/93‑1 (MQ=255)
                                                                             gTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTc            >  2:783633/1‑93 (MQ=255)
                                                                             gTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTc            >  1:3822828/1‑93 (MQ=255)
                                                                              ttGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCg           >  1:371510/1‑93 (MQ=255)
                                                                              ttGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGCTCg           >  2:2628213/1‑93 (MQ=255)
                                                                               tGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGa          <  1:746673/93‑1 (MQ=255)
                                                                                   gCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCAc      >  1:1412767/1‑93 (MQ=255)
                                                                                    cATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACt     >  2:1270261/1‑93 (MQ=255)
                                                                                     aTTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCCCGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACtt    >  2:2666620/1‑93 (MQ=255)
                                                                                     aTTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACtt    >  1:6253748/1‑93 (MQ=255)
                                                                                     aTTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACtt    >  1:2864928/1‑93 (MQ=255)
                                                                                      tttGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACttt   >  2:1601644/1‑93 (MQ=255)
                                                                                      tttGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACttt   >  1:2992073/1‑93 (MQ=255)
                                                                                      tttGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACttt   >  1:3273799/1‑93 (MQ=255)
                                                                                            |                                                                                        
AAGACCGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTA  >  NC_000913/2644506‑2644686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGG  >  NC_000913/2644511‑2644696
                                                                                            |                                                                                             
CGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGCC                                                                                              <  SRR4302533.4535475/94‑1 (MQ=60)
 GGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCA                                                                                            >  SRR4302533.4651575/1‑94 (MQ=60)
   CATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGC                                                                                          >  SRR4302533.1734873/1‑94 (MQ=60)
    ATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCC                                                                                         >  SRR4302533.5256069/1‑94 (MQ=60)
    ATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCC                                                                                         >  SRR4302533.5417222/1‑94 (MQ=60)
    ATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCC                                                                                         >  SRR4302533.1495655/1‑94 (MQ=60)
     TCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCA                                                                                        <  SRR4302533.2964754/94‑1 (MQ=60)
      CCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAG                                                                                       >  SRR4302533.3575510/1‑94 (MQ=60)
        AACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGAT                                                                                     >  SRR4302533.3806788/1‑94 (MQ=60)
        AACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGAT                                                                                     <  SRR4302533.3126159/94‑1 (MQ=60)
         ACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATG                                                                                    >  SRR4302533.2380426/1‑94 (MQ=60)
         ACGTAGTAGTGATATTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATG                                                                                    <  SRR4302533.3625004/94‑1 (MQ=60)
          CGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGG                                                                                   >  SRR4302533.4368716/1‑94 (MQ=60)
           GTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGG                                                                                  <  SRR4302533.4767500/94‑1 (MQ=60)
           GTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGG                                                                                  <  SRR4302533.5212145/94‑1 (MQ=60)
           GTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGG                                                                                  >  SRR4302533.1058118/1‑94 (MQ=60)
            TAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGT                                                                                 <  SRR4302533.4788563/94‑1 (MQ=60)
            TAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGT                                                                                 <  SRR4302533.3718156/94‑1 (MQ=60)
             AGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTT                                                                                >  SRR4302533.1775898/1‑94 (MQ=60)
              GTAGTGATTTTATCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTC                                                                               <  SRR4302533.3356931/94‑1 (MQ=60)
               TAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCT                                                                              >  SRR4302533.2968883/1‑94 (MQ=60)
                 GTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTC                                                                            <  SRR4302533.409032/94‑1 (MQ=60)
                 GTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTC                                                                            >  SRR4302533.4163426/1‑94 (MQ=60)
                 GTGATTTTGTCGCTTTTGCTTTTACCGAGAATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTC                                                                            >  SRR4302533.1290870/1‑94 (MQ=60)
                  TGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCC                                                                           <  SRR4302533.4771839/94‑1 (MQ=60)
                   TATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCC                                                                          >  SRR4302533.2130912/1‑94 (MQ=60)
                   GATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCC                                                                          <  SRR4302533.4472677/94‑1 (MQ=60)
                   GATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCC                                                                          >  SRR4302533.2855841/1‑94 (MQ=60)
                    ATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCT                                                                         <  SRR4302533.4859085/94‑1 (MQ=60)
                    ATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCT                                                                         >  SRR4302533.1573490/1‑94 (MQ=60)
                     TTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTT                                                                        <  SRR4302533.2012988/94‑1 (MQ=60)
                     TTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTT                                                                        >  SRR4302533.4396875/1‑94 (MQ=60)
                      TTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTG                                                                       <  SRR4302533.3748834/94‑1 (MQ=60)
                       TTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGA                                                                      <  SRR4302533.2334608/94‑1 (MQ=60)
                        TGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGAC                                                                     <  SRR4302533.1619305/94‑1 (MQ=60)
                          TCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTC                                                                   >  SRR4302533.3459238/1‑94 (MQ=60)
                           CGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCT                                                                  >  SRR4302533.1639914/1‑94 (MQ=60)
                             CTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTT                                                                >  SRR4302533.5775416/1‑94 (MQ=60)
                             CTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTT                                                                <  SRR4302533.3725365/94‑1 (MQ=60)
                             CTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTT                                                                >  SRR4302533.3226239/1‑94 (MQ=60)
                              TTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTT                                                               >  SRR4302533.4306093/1‑94 (MQ=60)
                               TTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTA                                                              >  SRR4302533.3178320/1‑94 (MQ=60)
                               TTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTA                                                              <  SRR4302533.4230963/94‑1 (MQ=60)
                                TTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTAC                                                             <  SRR4302533.387766/94‑1 (MQ=60)
                                  TCTTTTACCGAGCATCATGCGCGAAACCAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGCCTCTTTTACCT                                                           <  SRR4302533.1121696/94‑1 (MQ=60)
                                   CTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTG                                                          <  SRR4302533.2466045/94‑1 (MQ=60)
                                     TTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCT                                                        <  SRR4302533.4103268/94‑1 (MQ=60)
                                       TACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCA                                                      >  SRR4302533.4183143/1‑94 (MQ=60)
                                        ACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCAC                                                     <  SRR4302533.1397174/94‑1 (MQ=60)
                                          CGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGG                                                   >  SRR4302533.6084569/1‑94 (MQ=60)
                                           GAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGG                                                  >  SRR4302533.5527366/1‑94 (MQ=60)
                                           GAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGG                                                  <  SRR4302533.3656270/94‑1 (MQ=60)
                                           GAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGG                                                  >  SRR4302533.2759682/1‑94 (MQ=60)
                                            AGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGA                                                 >  SRR4302533.5694683/1‑94 (MQ=60)
                                             GCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGAC                                                >  SRR4302533.1823422/1‑94 (MQ=60)
                                             GCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGAC                                                <  SRR4302533.1968646/94‑1 (MQ=60)
                                             GCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGAC                                                <  SRR4302533.5269952/94‑1 (MQ=60)
                                              CATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACC                                               >  SRR4302533.5382946/1‑94 (MQ=60)
                                               ATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCCTTTGTC‑GGGGGCCAGCCAGATTGGGTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCA                                              >  SRR4302533.77072/1‑94 (MQ=60)
                                               ATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCA                                              >  SRR4302533.2258835/1‑94 (MQ=60)
                                               ATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCA                                              >  SRR4302533.6247920/1‑94 (MQ=60)
                                               ATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCA                                              >  SRR4302533.5619992/1‑94 (MQ=60)
                                               ATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCA                                              >  SRR4302533.2566716/1‑94 (MQ=60)
                                                TCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCAC                                             <  SRR4302533.5883787/94‑1 (MQ=60)
                                                 CATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACA                                            <  SRR4302533.2139040/94‑1 (MQ=60)
                                                 CATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACA                                            <  SRR4302533.329981/94‑1 (MQ=60)
                                                 CATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACA                                            <  SRR4302533.2496421/94‑1 (MQ=60)
                                                       CGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTT                                      <  SRR4302533.1010063/94‑1 (MQ=60)
                                                       CGAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTT                                      >  SRR4302533.4464208/1‑94 (MQ=60)
                                                        GAAAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGccctttttacttgctcccggacccacaccccttc                                     >  SRR4302533.2688655/1‑60 (MQ=60)
                                                          AAACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCA                                   >  SRR4302533.1228828/1‑94 (MQ=60)
                                                            ACAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACG                                 <  SRR4302533.471934/94‑1 (MQ=60)
                                                            ACAGCGGTGCCAGTTGCGGCATTTGGC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACG                                 <  SRR4302533.2434758/94‑1 (MQ=60)
                                                             CAGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGG                                <  SRR4302533.3724778/94‑1 (MQ=60)
                                                              AGCGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGC                               >  SRR4302533.106210/1‑94 (MQ=60)
                                                                CGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCA                             >  SRR4302533.5368787/1‑94 (MQ=60)
                                                                CGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCA                             >  SRR4302533.5219522/1‑94 (MQ=60)
                                                                CGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCA                             <  SRR4302533.4534085/94‑1 (MQ=60)
                                                                CGGTGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCA                             <  SRR4302533.1856831/94‑1 (MQ=60)
                                                                CGGTGCCAGTTGCGACATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCA                             <  SRR4302533.5507574/94‑1 (MQ=60)
                                                                   TGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCC                          >  SRR4302533.373565/1‑94 (MQ=60)
                                                                   TGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCC                          <  SRR4302533.646531/94‑1 (MQ=60)
                                                                   TGCCAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCC                          <  SRR4302533.5530925/94‑1 (MQ=60)
                                                                      CAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTT                       <  SRR4302533.911576/94‑1 (MQ=60)
                                                                      CAGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTT                       <  SRR4302533.2703626/94‑1 (MQ=60)
                                                                       AGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTC                      <  SRR4302533.4738726/94‑1 (MQ=60)
                                                                       AGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTC                      >  SRR4302533.3846199/1‑94 (MQ=60)
                                                                       AGTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTC                      <  SRR4302533.5048312/94‑1 (MQ=60)
                                                                        GTTGCGGCATTTGTCGGGGG‑‑CAGGCAGATGGGGTCTTCCCTTGACTCTTTTACCTGCTCACCGGAACACACACCTTGCACGGCCATCCGTTCGG                    >  SRR4302533.2924245/1‑94 (MQ=60)
                                                                        GTTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCG                     >  SRR4302533.373751/1‑94 (MQ=60)
                                                                         TTGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGA                    >  SRR4302533.3849359/1‑94 (MQ=60)
                                                                          TGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAG                   <  SRR4302533.4347244/94‑1 (MQ=60)
                                                                          TGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAG                   <  SRR4302533.751067/94‑1 (MQ=60)
                                                                          TGCGGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAG                   >  SRR4302533.1463211/1‑94 (MQ=60)
                                                                             GGCATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCAC                >  SRR4302533.1421311/1‑94 (MQ=60)
                                                                               CATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTT              >  SRR4302533.6291152/1‑94 (MQ=60)
                                                                               CATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTT              >  SRR4302533.2882554/1‑94 (MQ=60)
                                                                                ATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTT             >  SRR4302533.3294007/1‑94 (MQ=60)
                                                                                ATTTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTT             >  SRR4302533.3010368/1‑94 (MQ=60)
                                                                                  TTGTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTAT           >  SRR4302533.4756019/1‑94 (MQ=60)
                                                                                    GTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTA         <  SRR4302533.3994828/94‑1 (MQ=60)
                                                                                    GTC‑GGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTA         <  SRR4302533.3854632/94‑1 (MQ=60)
                                                                                      tcGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTAC        <  SRR4302533.2913114/92‑1 (MQ=60)
                                                                                      tcGGGGGCCAGCCAGATGGCTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGCTCGAGCACTTTATTAC        <  SRR4302533.5726066/92‑1 (MQ=60)
                                                                                       CGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACG       >  SRR4302533.1407119/1‑94 (MQ=60)
                                                                                         GGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCG     <  SRR4302533.2677430/94‑1 (MQ=60)
                                                                                         GGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCG     <  SRR4302533.548597/94‑1 (MQ=60)
                                                                                          GGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGC    >  SRR4302533.1718464/1‑94 (MQ=60)
                                                                                           GGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCG   >  SRR4302533.3251302/1‑94 (MQ=60)
                                                                                            GCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGG  <  SRR4302533.4662914/94‑1 (MQ=60)
                                                                                            |                                                                                             
CGGCATCCAACGTAGTAGTGATTTTGTCGCTTTTGCTTTTACCGAGCATCATGCGCGAAAACAGCGGTGCCAGTTGCGGCATTTGTCGGGGGGCCAGCCAGATGGGTTCTTCCCTTGACTCTTTTACCTGCTCACGGGACCACACACCTTGCACGGCCATCCGTTCGAGCACTTTATTACGCGCGG  >  NC_000913/2644511‑2644696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑