Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,645,360 T→C I1650V (ATT→GTT)  b2520 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,645,3600TC99.1% 748.2 / ‑4.4 232I1650V (ATT→GTT) b2520hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (110/119);  total (112/120)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 248 total aligned reads displayed.

ACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCG  >  NC_000913/2645270‑2645449
                                                                                          |                                                                                         
 cGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACcag                                                                                        <  2:10026334/93‑1 (MQ=255)
 cGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACcag                                                                                        >  1:8027205/1‑93 (MQ=255)
 cGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACcag                                                                                        <  1:2414449/93‑1 (MQ=255)
  gCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACcagc                                                                                       <  1:2241518/93‑1 (MQ=255)
  gCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACcagc                                                                                       <  2:2822077/93‑1 (MQ=255)
  gCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACcagc                                                                                       <  2:836977/93‑1 (MQ=255)
   cTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACcagca                                                                                      <  2:5174348/93‑1 (MQ=255)
   cTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACcagca                                                                                      >  2:4038887/1‑93 (MQ=255)
   cTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACaagca                                                                                      >  1:8704667/1‑93 (MQ=255)
    tGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACcagcag                                                                                     <  1:5901158/93‑1 (MQ=255)
    tGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACcagcag                                                                                     <  2:1707231/93‑1 (MQ=255)
      cAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGAt                                                                                   <  1:8768331/93‑1 (MQ=255)
      cAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGAt                                                                                   <  1:8124882/93‑1 (MQ=255)
      cAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGAt                                                                                   <  2:4826835/93‑1 (MQ=255)
       aaGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATc                                                                                  <  2:3545050/93‑1 (MQ=255)
       aaGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGGTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATc                                                                                  >  2:1947075/1‑93 (MQ=255)
       aaGAAAAAGGAGAAAAGGCGCGGCTGCCAACGTTCTCCAGCAGCCGCGATTGGTTAACAAGCGAGGCATTTACCGTATGACAATCAGGAGGTc                                                                                  >  2:7051239/1‑93 (MQ=255)
         aaaaaaGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCtt                                                                                <  1:3450496/92‑1 (MQ=255)
          aaaaaGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTc                                                                               <  2:6341105/93‑1 (MQ=255)
           aaaaGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCa                                                                              <  1:504372/93‑1 (MQ=255)
            aaaGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAg                                                                             <  2:6736484/93‑1 (MQ=255)
            aaaGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAg                                                                             <  2:4546391/93‑1 (MQ=255)
              aGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGcc                                                                           >  2:5884502/1‑93 (MQ=255)
               ggAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCcg                                                                          >  1:596048/1‑93 (MQ=255)
               ggAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCcg                                                                          <  1:7224203/93‑1 (MQ=255)
               ggAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCcg                                                                          <  1:5188014/93‑1 (MQ=255)
                gagaAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCcgc                                                                         <  2:6470851/93‑1 (MQ=255)
                  gaAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCgcgc                                                                       >  2:3845707/1‑93 (MQ=255)
                  gaAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCgcgc                                                                       >  2:9428297/1‑93 (MQ=255)
                   aaaGGGCGCGGCTGCCAACGTTATCCAGCAGCCTCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGcc                                                                      <  1:3567567/93‑1 (MQ=255)
                   aaaGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGcc                                                                      >  1:544312/1‑93 (MQ=255)
                   aaaGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGcc                                                                      <  2:9818627/93‑1 (MQ=255)
                   aaaGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGcc                                                                      >  2:2402572/1‑93 (MQ=255)
                     aGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCgg                                                                    <  2:3160605/93‑1 (MQ=255)
                      gggCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGt                                                                   <  2:3364990/93‑1 (MQ=255)
                          gcgGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTcgcc                                                               <  1:4234971/93‑1 (MQ=255)
                          gcgGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTcgcc                                                               <  2:1397450/93‑1 (MQ=255)
                               tGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCa                                                          >  1:9454222/1‑93 (MQ=255)
                               caccAACGTGAGCCAGCGCCTGCGTTGGGTTAACAAGCGAGGCTTTTACGGTCTAACAACGGGCAGCTCTTCACCCGCGCCTGTCCCCCGCCa                                                          <  2:841401/91‑1 (MQ=255)
                                gCCAACTTTCTCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCAt                                                         <  2:7178819/93‑1 (MQ=255)
                                gCCAACTTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCAt                                                         <  1:1036929/93‑1 (MQ=255)
                                cccAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCAt                                                         <  2:7058467/92‑1 (MQ=255)
                                 ccAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCAtt                                                        <  2:9530088/93‑1 (MQ=255)
                                    aCGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTggg                                                     <  2:8731041/93‑1 (MQ=255)
                                       ttATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGaa                                                  >  1:1290276/1‑93 (MQ=255)
                                       ttATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGaa                                                  <  1:8417583/93‑1 (MQ=255)
                                       taaTCCCGCACCCGCGTTTGTTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGaa                                                  <  2:2001109/91‑1 (MQ=255)
                                        tATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAac                                                 >  1:76036/1‑93 (MQ=255)
                                          tCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCACCCGCCATTGGGGAacat                                               >  2:4208455/1‑93 (MQ=255)
                                            cagcagCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAacatac                                             >  1:8542156/1‑93 (MQ=255)
                                            cagcagCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAacatac                                             >  2:8093005/1‑93 (MQ=255)
                                             agcagcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAacataca                                            <  2:7476744/93‑1 (MQ=255)
                                             agcagcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAacataca                                            <  2:5010408/93‑1 (MQ=255)
                                              gcagcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAacatacat                                           >  2:662473/1‑93 (MQ=255)
                                              gcagcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAacatacat                                           <  2:3468007/93‑1 (MQ=255)
                                                agcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTg                                         >  2:8844587/1‑93 (MQ=255)
                                                 gcCTCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGa                                        <  2:5639425/93‑1 (MQ=255)
                                                 gcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGa                                        >  2:9785126/1‑93 (MQ=255)
                                                 gcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGa                                        >  1:4480414/1‑93 (MQ=255)
                                                  ccGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGAt                                       >  1:4391795/1‑93 (MQ=255)
                                                  ccGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGAt                                       <  1:4036377/93‑1 (MQ=255)
                                                   cgcgTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGAtt                                      >  2:249150/1‑93 (MQ=255)
                                                    gcgTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTc                                     >  2:8571069/1‑93 (MQ=255)
                                                     cgTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTcc                                    <  2:5319931/93‑1 (MQ=255)
                                                      gTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTcca                                   <  1:3635648/93‑1 (MQ=255)
                                                       tttGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATCCATTGATTcaac                                  >  2:2656164/1‑93 (MQ=255)
                                                          ggTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCAt                               <  1:9321832/93‑1 (MQ=255)
                                                           gTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATc                              >  2:3801455/1‑93 (MQ=255)
                                                             tAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCgg                            <  2:2300180/93‑1 (MQ=255)
                                                               aCAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGtt                          <  1:2095026/93‑1 (MQ=255)
                                                               aCAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGtt                          <  1:9666899/93‑1 (MQ=255)
                                                               aCAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGtt                          >  1:9114289/1‑93 (MQ=255)
                                                               aCAAGCGAGGCATTTACGGTCTGACAACCAGCAGATATTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGtt                          <  1:4225562/93‑1 (MQ=255)
                                                                cAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTg                         >  1:5682873/1‑93 (MQ=255)
                                                                 aaGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGc                        >  1:7802437/1‑93 (MQ=255)
                                                                   gCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCgg                      >  1:5316687/1‑93 (MQ=255)
                                                                   gCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCgg                      >  1:1408969/1‑93 (MQ=255)
                                                                   gCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCgg                      >  2:4266717/1‑93 (MQ=255)
                                                                      aGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTAc                   >  1:6982740/1‑93 (MQ=255)
                                                                      aGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTAc                   <  2:1835442/93‑1 (MQ=255)
                                                                      aGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTAc                   >  1:2185472/1‑93 (MQ=255)
                                                                           tttACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGAt              >  2:3196470/1‑93 (MQ=255)
                                                                           tttACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGAt              >  1:6301667/1‑93 (MQ=255)
                                                                           tttACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGAt              >  2:6670288/1‑93 (MQ=255)
                                                                           tttACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGAt              >  1:1790898/1‑93 (MQ=255)
                                                                           tttACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGAt              >  2:1544319/1‑93 (MQ=255)
                                                                           tttACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGAt              >  2:8494131/1‑93 (MQ=255)
                                                                           tttACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGAt              >  1:21276/1‑93 (MQ=255)
                                                                            ttACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATa             >  2:3239571/1‑93 (MQ=255)
                                                                            ttACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCG‑CCGCCATTGGGGGACATTCCATGATTCCAACATCGGGTGCGGGACCTGATaa            >  2:1409877/1‑92 (MQ=255)
                                                                            ttACGGGCTGACAACCAGCAGATATTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATa             <  2:6909610/93‑1 (MQ=255)
                                                                             tACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATAc            <  1:484300/93‑1 (MQ=255)
                                                                             tACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATAc            >  1:3194136/1‑93 (MQ=255)
                                                                               cGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGt          >  2:602920/1‑93 (MQ=255)
                                                                                ggTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGtt         <  2:8324192/93‑1 (MQ=255)
                                                                                ggTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGtt         <  1:2695541/93‑1 (MQ=255)
                                                                                  tCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTcc       <  1:4462487/93‑1 (MQ=255)
                                                                                  tCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTcc       >  1:9769633/1‑93 (MQ=255)
                                                                                   cTGACACCCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTccc      <  1:1576109/93‑1 (MQ=255)
                                                                                    tGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCg     >  2:293837/1‑93 (MQ=255)
                                                                                          |                                                                                         
ACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCG  >  NC_000913/2645270‑2645449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCA  >  NC_000913/2645267‑2645452
                                                                                             |                                                                                            
CCCACGCTGCGAGAAAAGGTATAAAGGGCGCGGCTGCCATCGTCATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAAC                                                                                              <  SRR4302540.2035138/94‑1 (MQ=60)
CCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAAC                                                                                              >  SRR4302540.9073647/1‑94 (MQ=60)
CCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAAC                                                                                              <  SRR4302540.3446792/94‑1 (MQ=60)
CCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAAC                                                                                              <  SRR4302540.697967/94‑1 (MQ=60)
CCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAAC                                                                                              <  SRR4302540.2862673/94‑1 (MQ=60)
CCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAAC                                                                                              >  SRR4302540.3725104/1‑94 (MQ=60)
CCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAAC                                                                                              >  SRR4302540.69058/1‑94 (MQ=60)
 CCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACC                                                                                             >  SRR4302540.1313255/1‑94 (MQ=60)
  CACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCA                                                                                            <  SRR4302540.9558931/94‑1 (MQ=60)
  CACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCA                                                                                            <  SRR4302540.9640456/94‑1 (MQ=60)
   ACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAG                                                                                           >  SRR4302540.8120552/1‑94 (MQ=60)
    CGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGC                                                                                          <  SRR4302540.2440473/94‑1 (MQ=60)
     GCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCA                                                                                         <  SRR4302540.2265608/94‑1 (MQ=60)
     GCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCA                                                                                         <  SRR4302540.1336026/94‑1 (MQ=60)
     GCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACAAGCA                                                                                         >  SRR4302540.8805708/1‑94 (MQ=60)
      CTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAG                                                                                        >  SRR4302540.2295080/1‑94 (MQ=60)
       TGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGA                                                                                       >  SRR4302540.3281192/1‑94 (MQ=60)
       TGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGA                                                                                       <  SRR4302540.5966324/94‑1 (MQ=60)
       TGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGA                                                                                       >  SRR4302540.9335765/1‑94 (MQ=60)
        GCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTTACAACCAGCAGAT                                                                                      >  SRR4302540.5119603/1‑94 (MQ=60)
        GCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGAT                                                                                      >  SRR4302540.9487077/1‑94 (MQ=60)
         CAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATC                                                                                     <  SRR4302540.8219181/94‑1 (MQ=60)
         CAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATC                                                                                     <  SRR4302540.8869961/94‑1 (MQ=60)
          AAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCT                                                                                    <  SRR4302540.8598769/94‑1 (MQ=60)
          AAGAAAAAGGAGAAAGGGCGCGGCGGCCATCGTTATCCAGCAGCCACGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCT                                                                                    <  SRR4302540.7247356/94‑1 (MQ=60)
            AAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTC                                                                                  <  SRR4302540.3488030/94‑1 (MQ=60)
             AAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCA                                                                                 <  SRR4302540.4924509/94‑1 (MQ=60)
              AAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAG                                                                                <  SRR4302540.509774/94‑1 (MQ=60)
              AAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAG                                                                                >  SRR4302540.5881792/1‑94 (MQ=60)
               AAAGGAGAAAGGGCGCGGCTGCCAGCGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCAATTACGGTCTGACAACCAGCAGATCTTCAGC                                                                               <  SRR4302540.9070182/94‑1 (MQ=60)
               AAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGC                                                                               >  SRR4302540.9707834/1‑94 (MQ=60)
               AAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGC                                                                               <  SRR4302540.2510849/94‑1 (MQ=60)
                AAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCC                                                                              <  SRR4302540.2113424/94‑1 (MQ=60)
                 AGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCG                                                                             >  SRR4302540.602448/1‑94 (MQ=60)
                  GGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGC                                                                            <  SRR4302540.7304744/94‑1 (MQ=60)
                  GGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGC                                                                            <  SRR4302540.5244493/94‑1 (MQ=60)
                   GAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCG                                                                           <  SRR4302540.5493371/94‑1 (MQ=60)
                     GAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCC                                                                         >  SRR4302540.550231/1‑94 (MQ=60)
                     GAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAAACAGCAGATCTTAAGCCGCGCC                                                                         >  SRR4302540.5404410/1‑94 (MQ=60)
                      AAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCTCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCG                                                                        <  SRR4302540.3606535/94‑1 (MQ=60)
                       AAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGG                                                                       <  SRR4302540.1934911/94‑1 (MQ=60)
                        AGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGT                                                                      <  SRR4302540.1617799/94‑1 (MQ=60)
                        AGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCCCCGGC                                                                      >  SRR4302540.2256385/1‑94 (MQ=60)
                            CGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCC                                                                  <  SRR4302540.6754671/94‑1 (MQ=60)
                            CGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCC                                                                  <  SRR4302540.3541856/94‑1 (MQ=60)
                             GCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCC                                                                 <  SRR4302540.3528325/94‑1 (MQ=60)
                             GCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCC                                                                 <  SRR4302540.4281054/94‑1 (MQ=60)
                             GCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCC                                                                 >  SRR4302540.4497281/1‑94 (MQ=60)
                             GCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCC                                                                 <  SRR4302540.4517184/94‑1 (MQ=60)
                               GGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGC                                                               >  SRR4302540.902647/1‑94 (MQ=60)
                                GCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCC                                                              >  SRR4302540.5102221/1‑94 (MQ=60)
                                 CTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCA                                                             >  SRR4302540.9566431/1‑94 (MQ=60)
                                   GCCAACTTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATT                                                           <  SRR4302540.1047473/94‑1 (MQ=60)
                                     caagtttacccagcagccgctgtgGGTTAACAAGCGAGGCATTTGCGTTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGG                                                         <  SRR4302540.7848380/70‑1 (MQ=60)
                                     CAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGG                                                         <  SRR4302540.7848379/94‑1 (MQ=60)
                                     CAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGG                                                         <  SRR4302540.9638548/94‑1 (MQ=60)
                                      AACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGG                                                        <  SRR4302540.8783499/94‑1 (MQ=60)
                                      AACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGG                                                        <  SRR4302540.3227128/94‑1 (MQ=60)
                                       ACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTAACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGG                                                       <  SRR4302540.4274673/94‑1 (MQ=60)
                                         GTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAA                                                     >  SRR4302540.1303610/1‑94 (MQ=60)
                                         GTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAA                                                     <  SRR4302540.6934682/94‑1 (MQ=60)
                                          TTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAAC                                                    <  SRR4302540.8515292/94‑1 (MQ=60)
                                          TTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAAC                                                    >  SRR4302540.76825/1‑94 (MQ=60)
                                            ATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGCTCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACAT                                                  >  SRR4302540.10023828/1‑94 (MQ=60)
                                              CCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATAC                                                >  SRR4302540.8641406/1‑94 (MQ=60)
                                              CCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATAC                                                <  SRR4302540.9860146/94‑1 (MQ=60)
                                                AGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACAT                                              <  SRR4302540.438480/94‑1 (MQ=60)
                                                   AGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGA                                           >  SRR4302540.2150834/1‑94 (MQ=60)
                                                   AGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGA                                           >  SRR4302540.4529001/1‑94 (MQ=60)
                                                    GCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGAT                                          >  SRR4302540.8247497/1‑94 (MQ=60)
                                                    GCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGAT                                          >  SRR4302540.4439456/1‑94 (MQ=60)
                                                     CCTCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATT                                         <  SRR4302540.4079898/94‑1 (MQ=60)
                                                     CCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATT                                         <  SRR4302540.4079897/94‑1 (MQ=60)
                                                      CGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTC                                        >  SRR4302540.8698429/1‑94 (MQ=60)
                                                       GCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCC                                       >  SRR4302540.5990851/1‑94 (MQ=60)
                                                         GTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTTATTCCAC                                     >  SRR4302540.8390408/1‑94 (MQ=60)
                                                         GTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCAC                                     <  SRR4302540.3675445/94‑1 (MQ=60)
                                                          TTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACC                                    >  SRR4302540.528247/1‑94 (MQ=60)
                                                           TTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCA                                   <  SRR4302540.1545967/94‑1 (MQ=60)
                                                           TTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCA                                   <  SRR4302540.4277483/94‑1 (MQ=60)
                                                             GGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATC                                 >  SRR4302540.9796635/1‑94 (MQ=60)
                                                             GGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATC                                 <  SRR4302540.8071529/94‑1 (MQ=60)
                                                             GGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATC                                 <  SRR4302540.9433050/94‑1 (MQ=60)
                                                              GTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCG                                <  SRR4302540.9550218/94‑1 (MQ=60)
                                                                 AACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTT                             <  SRR4302540.1587924/94‑1 (MQ=60)
                                                                 AACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTT                             >  SRR4302540.9223834/1‑94 (MQ=60)
                                                                  ACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTG                            <  SRR4302540.9780670/94‑1 (MQ=60)
                                                                  ACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTG                            >  SRR4302540.5745441/1‑94 (MQ=60)
                                                                  ACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTG                            <  SRR4302540.2117524/94‑1 (MQ=60)
                                                                  ACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATATTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTG                            <  SRR4302540.4271562/94‑1 (MQ=60)
                                                                   CAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGCACATACATTAATTCCACCATCGTTTGC                           >  SRR4302540.7893627/1‑94 (MQ=60)
                                                                   CAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGC                           >  SRR4302540.7893625/1‑94 (MQ=60)
                                                                     AGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGG                         >  SRR4302540.1424146/1‑94 (MQ=60)
                                                                     AGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGG                         >  SRR4302540.5374899/1‑94 (MQ=60)
                                                                      GCGAGCCATTTACGCTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGT                        <  SRR4302540.7364/94‑1 (MQ=60)
                                                                        GAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTAC                      >  SRR4302540.2209063/1‑94 (MQ=60)
                                                                        GAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTAC                      >  SRR4302540.2153586/1‑94 (MQ=60)
                                                                        GAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTAC                      >  SRR4302540.7060293/1‑94 (MQ=60)
                                                                         AGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACC                     <  SRR4302540.2409545/94‑1 (MQ=60)
                                                                             CTTTACGCTCTGACAACCAGCAGATCTTCGGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGAT                 <  SRR4302540.4794242/94‑1 (MQ=60)
                                                                             ATTTACGGTCTGACAACCAGCAGTTCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGAT                 <  SRR4302540.2143384/94‑1 (MQ=60)
                                                                             ATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGAT                 >  SRR4302540.21511/1‑94 (MQ=60)
                                                                             ATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGAT                 >  SRR4302540.1810363/1‑94 (MQ=60)
                                                                             ATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGAT                 >  SRR4302540.6371847/1‑94 (MQ=60)
                                                                             ATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGAT                 >  SRR4302540.6314490/1‑94 (MQ=60)
                                                                             ATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGAT                 >  SRR4302540.4000648/1‑94 (MQ=60)
                                                                              TTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATA                >  SRR4302540.2949350/1‑94 (MQ=60)
                                                                               TTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATAC               >  SRR4302540.3228825/1‑94 (MQ=60)
                                                                               TTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATAC               <  SRR4302540.1272755/94‑1 (MQ=60)
                                                                                TACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACG              <  SRR4302540.489479/94‑1 (MQ=60)
                                                                                  CGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTT            <  SRR4302540.5691556/94‑1 (MQ=60)
                                                                                  CGGTCTGACAACCAGCAGACCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTT            <  SRR4302540.4057556/94‑1 (MQ=60)
                                                                                   GGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTC           <  SRR4302540.2724642/94‑1 (MQ=60)
                                                                                    GTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCC          >  SRR4302540.9884184/1‑94 (MQ=60)
                                                                                    GTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCC          >  SRR4302540.8827720/1‑94 (MQ=60)
                                                                                    GTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCC          <  SRR4302540.3656700/94‑1 (MQ=60)
                                                                                     TCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCC         <  SRR4302540.4510862/94‑1 (MQ=60)
                                                                                      CTGACACCCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCG        <  SRR4302540.1593354/94‑1 (MQ=60)
                                                                                        GACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGC      <  SRR4302540.4497680/94‑1 (MQ=60)
                                                                                        GACAACCAGCAGACCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGC      <  SRR4302540.7512239/94‑1 (MQ=60)
                                                                                         ACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCG     <  SRR4302540.6017215/94‑1 (MQ=60)
                                                                                         ACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCG     >  SRR4302540.4727244/1‑94 (MQ=60)
                                                                                           AACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTC   <  SRR4302540.7386852/94‑1 (MQ=60)
                                                                                           AACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTC   >  SRR4302540.554838/1‑94 (MQ=60)
                                                                                           AACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTC   <  SRR4302540.5139417/94‑1 (MQ=60)
                                                                                            ACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCA  <  SRR4302540.8718914/94‑1 (MQ=60)
                                                                                             |                                                                                            
CCCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCA  >  NC_000913/2645267‑2645452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑