Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,645,360 T→C I1650V (ATT→GTT)  b2520 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,645,3600TC100.0% 606.3 / NA 186I1650V (ATT→GTT) b2520hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (89/97);  total (89/97)

Only 100 of 203 total aligned reads displayed.

ACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGC  >  NC_000913/2645270‑2645448
                                                                                          |                                                                                        
 cGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGGCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACcag                                                                                       <  1:225420/93‑1 (MQ=255)
  gCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACcagc                                                                                      <  2:4419307/93‑1 (MQ=255)
   gtGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTTACAACcagca                                                                                     <  2:2381896/92‑1 (MQ=255)
   cTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACcagca                                                                                     <  2:1148697/93‑1 (MQ=255)
   cTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACcagca                                                                                     <  2:2784866/93‑1 (MQ=255)
     gCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGa                                                                                   <  1:1921842/93‑1 (MQ=255)
         gAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCtt                                                                               >  2:620905/1‑93 (MQ=255)
         gAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCtt                                                                               <  1:2747567/93‑1 (MQ=255)
          aaaaaGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTc                                                                              <  2:762662/93‑1 (MQ=255)
          aaaaaGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTc                                                                              <  1:175721/93‑1 (MQ=255)
          aaaaaGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTc                                                                              <  1:3903813/93‑1 (MQ=255)
           aaaaGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCa                                                                             <  1:5134342/93‑1 (MQ=255)
            aaaGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAg                                                                            <  1:3849517/93‑1 (MQ=255)
             aaGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGc                                                                           <  1:664338/93‑1 (MQ=255)
              aGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGcc                                                                          <  2:4953896/93‑1 (MQ=255)
              aGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGcc                                                                          >  2:5768456/1‑93 (MQ=255)
              aGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGcc                                                                          <  2:433740/93‑1 (MQ=255)
              aGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGcc                                                                          <  2:6175202/93‑1 (MQ=255)
               ggAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCcg                                                                         >  1:2146737/1‑93 (MQ=255)
               ggAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTAGGTTAACCAGCGAGGCATTTACGGTCTGACAACCCGCAGATCTTCAGCcg                                                                         >  1:4431470/1‑93 (MQ=255)
                 agaAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCcgcg                                                                       <  1:347035/93‑1 (MQ=255)
                  gaAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCgcgc                                                                      >  2:5490917/1‑93 (MQ=255)
                     aGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCgg                                                                   <  1:2017978/93‑1 (MQ=255)
                     aGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCgg                                                                   >  1:1404687/1‑93 (MQ=255)
                      gggCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGt                                                                  <  2:3141532/93‑1 (MQ=255)
                       ggCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACCACCAGCAGATCTTCCGCCGCGGCGGTc                                                                 >  1:2576345/1‑93 (MQ=255)
                          gcgGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTcgcc                                                              <  2:2668206/93‑1 (MQ=255)
                          gcgGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGGcgcc                                                              >  1:6288834/1‑93 (MQ=255)
                           cgGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTcgccc                                                             <  1:264530/93‑1 (MQ=255)
                             gCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTcgcccgc                                                           <  1:1225368/93‑1 (MQ=255)
                               tGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCa                                                         >  2:5124852/1‑93 (MQ=255)
                                   aaCGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTgg                                                     >  2:4136/1‑93 (MQ=255)
                                   aaCGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTgg                                                     <  2:875761/93‑1 (MQ=255)
                                   aaCGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTgg                                                     >  2:4908846/1‑93 (MQ=255)
                                   aaCGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTgg                                                     >  1:3711414/1‑93 (MQ=255)
                                    aCGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTggg                                                    <  1:3093513/93‑1 (MQ=255)
                                      gTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGa                                                  <  2:1318740/93‑1 (MQ=255)
                                       ttATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGaa                                                 >  1:3798600/1‑93 (MQ=255)
                                        tATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAac                                                >  2:1482519/1‑93 (MQ=255)
                                         aTCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAaca                                               <  2:1971455/93‑1 (MQ=255)
                                          tCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAacat                                              >  1:5896189/1‑93 (MQ=255)
                                          tCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAacat                                              >  2:3966064/1‑93 (MQ=255)
                                           ccAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCACCGGTCGCCCGCCACTGGGGAacata                                             >  1:5579125/1‑93 (MQ=255)
                                            cggcaccCTCGTTTGGTTAACAAGCGGGGCATTGCCGGTCTGACAACCAGCAGCTCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAacatac                                            <  2:1228895/91‑1 (MQ=255)
                                             agcagcCTCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAacataca                                           <  1:82492/93‑1 (MQ=255)
                                             agcagcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAacataca                                           <  1:2282535/93‑1 (MQ=255)
                                             agcagcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAacataca                                           >  2:5592167/1‑93 (MQ=255)
                                              gcagcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCTCCGGTCGCCCGCCATTGGGGCacatacat                                          >  2:4527675/1‑93 (MQ=255)
                                              gcagcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGTTCGCCCGCCATTGGGGAacatacat                                          >  2:4811079/1‑93 (MQ=255)
                                               gagcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACAtt                                         <  2:5015689/92‑1 (MQ=255)
                                               cagtCGCGTTTGGTTAACGATCAAGGCATTTACGGTCTGACAACCAGCAGATCTTCCGCCGCGCCGGTCGCCCGCCATTGGGGAACATACAtt                                         <  2:3204801/93‑1 (MQ=255)
                                               cagcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACAtt                                         <  1:2200198/93‑1 (MQ=255)
                                               cagcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACAtt                                         <  2:2990471/93‑1 (MQ=255)
                                                 gcCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGa                                       <  1:3632562/93‑1 (MQ=255)
                                                 gcCGCGTTTGGTGAACAAGCGAGGCATTGACGGTCTGACAACCAGCAGATCTTCAGCCGCGCGGGTCGCCCGCCATTGGGGAACATACATTGa                                       <  2:4859439/93‑1 (MQ=255)
                                                  ccGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGAt                                      >  1:4647706/1‑93 (MQ=255)
                                                     cgTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTcc                                   >  1:4493247/1‑93 (MQ=255)
                                                     cgTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTcc                                   >  2:6057194/1‑93 (MQ=255)
                                                     cgTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTcc                                   <  2:1680647/93‑1 (MQ=255)
                                                      gTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTcca                                  >  1:3818941/1‑93 (MQ=255)
                                                      gTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTcca                                  >  1:1974282/1‑93 (MQ=255)
                                                      gTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTcca                                  >  2:1641649/1‑93 (MQ=255)
                                                      gTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTcca                                  >  1:4126364/1‑93 (MQ=255)
                                                      gTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTcca                                  <  2:2833724/93‑1 (MQ=255)
                                                       tttGGTTAACAAGCGAGGCATTTACGTTCTGACACCCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTccac                                 <  1:5699500/93‑1 (MQ=255)
                                                        ttGGTTAACAAGCGAGGCATTTACGTTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTccacc                                <  2:3628684/93‑1 (MQ=255)
                                                        ttGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTcgacc                                >  1:5393480/1‑93 (MQ=255)
                                                        ttGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTccacc                                <  2:530928/93‑1 (MQ=255)
                                                        ttGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTccacc                                <  1:5745035/93‑1 (MQ=255)
                                                        ttGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTccacc                                <  1:299001/93‑1 (MQ=255)
                                                        tgggTAAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGATCGCCCGCCATTGGGGAACATACATTGATTccacc                                <  1:1710963/91‑1 (MQ=255)
                                                         tGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTccacca                               <  1:4593529/93‑1 (MQ=255)
                                                         tGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTccacca                               <  1:1045481/93‑1 (MQ=255)
                                                              aaCAAGCGAGTCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGt                          <  1:4935448/93‑1 (MQ=255)
                                                              aaCAAGCGAGGCATTTACGCTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGt                          <  1:358230/93‑1 (MQ=255)
                                                               aCAAGCGAGTCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGtt                         <  1:2910618/93‑1 (MQ=255)
                                                               aCAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGtt                         <  2:5557501/93‑1 (MQ=255)
                                                                 aaGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGc                       <  2:4726276/93‑1 (MQ=255)
                                                                   gCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCgg                     <  1:2834943/93‑1 (MQ=255)
                                                                     gAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTa                   >  1:1793195/1‑93 (MQ=255)
                                                                     gAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTa                   >  2:1040879/1‑93 (MQ=255)
                                                                      aGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTAc                  <  1:1332454/93‑1 (MQ=255)
                                                                       ggCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTAcc                 <  2:1763033/93‑1 (MQ=255)
                                                                         cATTTACGGTCTGACAACCGGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTg               <  1:4718275/93‑1 (MQ=255)
                                                                          aTTTCCGGTCTGAGAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGa              <  2:4356198/93‑1 (MQ=255)
                                                                          aTTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGa              >  2:4584484/1‑93 (MQ=255)
                                                                           tttACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGAt             >  2:3892001/1‑93 (MQ=255)
                                                                           tttACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGAt             >  1:1595035/1‑93 (MQ=255)
                                                                           tttACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGAt             >  1:6133859/1‑93 (MQ=255)
                                                                                ggTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGtt        >  1:4783715/1‑93 (MQ=255)
                                                                                ggTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGtt        >  1:3290533/1‑93 (MQ=255)
                                                                                 gTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTc       <  2:3687933/93‑1 (MQ=255)
                                                                                  tCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTcc      <  2:402750/93‑1 (MQ=255)
                                                                                   cTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTccc     <  2:54652/93‑1 (MQ=255)
                                                                                    taaCAACCGGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCg    <  2:2665131/91‑1 (MQ=255)
                                                                                    tGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCg    <  2:3316459/93‑1 (MQ=255)
                                                                                    tGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCg    >  2:4261011/1‑93 (MQ=255)
                                                                                     gACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATGCATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCgg   <  1:3382004/93‑1 (MQ=255)
                                                                                     gACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCgg   <  2:1791217/93‑1 (MQ=255)
                                                                                     gACAACCAGCAGACCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCgg   <  1:2077358/93‑1 (MQ=255)
                                                                                          |                                                                                        
ACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGC  >  NC_000913/2645270‑2645448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCAC  >  NC_000913/2645268‑2645453
                                                                                            |                                                                                             
CCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACC                                                                                              <  SRR4302533.5890220/94‑1 (MQ=60)
CCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACC                                                                                              <  SRR4302533.3576153/94‑1 (MQ=60)
CCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACC                                                                                              <  SRR4302533.3198401/94‑1 (MQ=60)
 CACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCA                                                                                             <  SRR4302533.5907144/94‑1 (MQ=60)
 CACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCA                                                                                             >  SRR4302533.4630481/1‑94 (MQ=60)
  ACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAG                                                                                            <  SRR4302533.1187384/94‑1 (MQ=60)
  ACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAG                                                                                            <  SRR4302533.5859477/94‑1 (MQ=60)
   CGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGGCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGC                                                                                           <  SRR4302533.226826/94‑1 (MQ=60)
   CGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGC                                                                                           <  SRR4302533.3390911/94‑1 (MQ=60)
    GCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCA                                                                                          >  SRR4302533.704414/1‑94 (MQ=60)
    GCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCA                                                                                          <  SRR4302533.4710209/94‑1 (MQ=60)
     CTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAG                                                                                         <  SRR4302533.3085626/94‑1 (MQ=60)
     CTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAG                                                                                         >  SRR4302533.3813017/1‑94 (MQ=60)
      TGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGA                                                                                        >  SRR4302533.4799275/1‑94 (MQ=60)
       GCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGAT                                                                                       <  SRR4302533.1740057/94‑1 (MQ=60)
       GCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGAT                                                                                       <  SRR4302533.1933571/94‑1 (MQ=60)
         AAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCT                                                                                     <  SRR4302533.4463244/94‑1 (MQ=60)
         AAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCT                                                                                     >  SRR4302533.2019988/1‑94 (MQ=60)
           GAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTC                                                                                   <  SRR4302533.2764410/94‑1 (MQ=60)
            AAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCA                                                                                  <  SRR4302533.3927655/94‑1 (MQ=60)
            AAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCA                                                                                  <  SRR4302533.176827/94‑1 (MQ=60)
            AAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCA                                                                                  <  SRR4302533.2214606/94‑1 (MQ=60)
            AAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTGTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCA                                                                                  <  SRR4302533.3927656/94‑1 (MQ=60)
             AAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAG                                                                                 <  SRR4302533.5165362/94‑1 (MQ=60)
             AAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAG                                                                                 <  SRR4302533.541460/94‑1 (MQ=60)
              AAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGC                                                                                <  SRR4302533.3873025/94‑1 (MQ=60)
               AAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCC                                                                               <  SRR4302533.668260/94‑1 (MQ=60)
               AAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCC                                                                               <  SRR4302533.2288401/94‑1 (MQ=60)
                AGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCG                                                                              >  SRR4302533.2159959/1‑94 (MQ=60)
                AGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTAGGTTAACCAGCGAGGCATTTACGGTCTGACAACCCGCAGATCTTCAGCCG                                                                              >  SRR4302533.4458260/1‑94 (MQ=60)
                 GGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGC                                                                             >  SRR4302533.1516228/1‑94 (MQ=60)
                  GAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCG                                                                            <  SRR4302533.2676537/94‑1 (MQ=60)
                   AGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGC                                                                           <  SRR4302533.349154/94‑1 (MQ=60)
                   AGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGC                                                                           >  SRR4302533.2557286/1‑94 (MQ=60)
                   AGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGATAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGC                                                                           >  SRR4302533.3411246/1‑94 (MQ=60)
                    GAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCC                                                                          <  SRR4302533.2200007/94‑1 (MQ=60)
                     AAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCG                                                                         <  SRR4302533.2782842/94‑1 (MQ=60)
                      AAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGG                                                                        >  SRR4302533.6280527/1‑94 (MQ=60)
                      AAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGG                                                                        >  SRR4302533.1694602/1‑94 (MQ=60)
                      AAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGG                                                                        >  SRR4302533.3239263/1‑94 (MQ=60)
                      AAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGG                                                                        >  SRR4302533.1413186/1‑94 (MQ=60)
                       AGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGT                                                                       >  SRR4302533.421598/1‑94 (MQ=60)
                       AGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGT                                                                       <  SRR4302533.2030412/94‑1 (MQ=60)
                        GGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACCACCAGCAGATCTTCCGCCGCGGCGGTC                                                                      >  SRR4302533.2592195/1‑94 (MQ=60)
                        GGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTC                                                                      <  SRR4302533.5443386/94‑1 (MQ=60)
                           CGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGGCGCC                                                                   >  SRR4302533.6326346/1‑94 (MQ=60)
                            GCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCC                                                                  >  SRR4302533.2636702/1‑94 (MQ=60)
                             CGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCG                                                                 <  SRR4302533.266136/94‑1 (MQ=60)
                             CGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCG                                                                 <  SRR4302533.2976722/94‑1 (MQ=60)
                              GGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGC                                                                >  SRR4302533.5736347/1‑94 (MQ=60)
                               GCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCC                                                               <  SRR4302533.1232711/94‑1 (MQ=60)
                                CGGCCAACTTTACCCAGCACCCGCGTTTGGTAAACAAGCGAGGCATTTACGGTCTGACACCCGGCAGATCTTCACCCGCGCCGGTCGCCCGCCA                                                              <  SRR4302533.5541460/94‑1 (MQ=60)
                                 TGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTCCGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCAT                                                             <  SRR4302533.1235868/94‑1 (MQ=60)
                                  GCCAACGTTATCCAGCAGCGGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATT                                                            <  SRR4302533.3844855/94‑1 (MQ=60)
                                   CCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTG                                                           <  SRR4302533.4432261/94‑1 (MQ=60)
                                   CCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTG                                                           >  SRR4302533.177681/1‑94 (MQ=60)
                                    caacnanatcgagcatcccctTTTGGTTAACAAGCAAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGG                                                          <  SRR4302533.5589124/73‑1 (MQ=60)
                                    CAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGG                                                          <  SRR4302533.4959293/94‑1 (MQ=60)
                                    CAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGG                                                          >  SRR4302533.3734205/1‑94 (MQ=60)
                                    CAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGACGGTCGCCCGCCATTGG                                                          >  SRR4302533.3830434/1‑94 (MQ=60)
                                     AACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGG                                                         >  SRR4302533.5684861/1‑94 (MQ=60)
                                      ACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGG                                                        <  SRR4302533.3112531/94‑1 (MQ=60)
                                        GTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAA                                                      >  SRR4302533.4591815/1‑94 (MQ=60)
                                        GTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAA                                                      >  SRR4302533.3821853/1‑94 (MQ=60)
                                           ATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACAT                                                   >  SRR4302533.5931793/1‑94 (MQ=60)
                                            TCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCACCGGTCGCCCGCCACTGGGGAACATA                                                  >  SRR4302533.5612842/1‑94 (MQ=60)
                                               AGCAGCCTCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACAT                                               <  SRR4302533.83015/94‑1 (MQ=60)
                                               AGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACAT                                               <  SRR4302533.2296665/94‑1 (MQ=60)
                                                 CAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTG                                             >  SRR4302533.973392/1‑94 (MQ=60)
                                                 CAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTG                                             <  SRR4302533.2213752/94‑1 (MQ=60)
                                                  AGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGA                                            >  SRR4302533.2057150/1‑94 (MQ=60)
                                                  AGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGA                                            >  SRR4302533.1352655/1‑94 (MQ=60)
                                                   GCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGAT                                           <  SRR4302533.3654910/94‑1 (MQ=60)
                                                   GCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGAT                                           >  SRR4302533.4675868/1‑94 (MQ=60)
                                                      GCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCC                                        >  SRR4302533.4520460/1‑94 (MQ=60)
                                                       CGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCA                                       >  SRR4302533.1986370/1‑94 (MQ=60)
                                                       CGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCA                                       >  SRR4302533.3904063/1‑94 (MQ=60)
                                                       CGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCA                                       >  SRR4302533.3842295/1‑94 (MQ=60)
                                                       CGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCA                                       >  SRR4302533.4151412/1‑94 (MQ=60)
                                                         TTTGGTTAACAAGCGAGGCATTTACGTTCTGACACCCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACC                                     <  SRR4302533.5733920/94‑1 (MQ=60)
                                                         TTTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCGACC                                     >  SRR4302533.5425884/1‑94 (MQ=60)
                                                          TTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCA                                    <  SRR4302533.5779701/94‑1 (MQ=60)
                                                          TTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCA                                    >  SRR4302533.2853823/1‑94 (MQ=60)
                                                          TTGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCA                                    <  SRR4302533.300832/94‑1 (MQ=60)
                                                          TGGGTAAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGATCGCCCGCCATTGGGGAACATACATTGATTCCACCA                                    <  SRR4302533.1721332/94‑1 (MQ=60)
                                                           TGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCAT                                   <  SRR4302533.1051626/94‑1 (MQ=60)
                                                           TGGTTAACAAGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCAT                                   <  SRR4302533.4621354/94‑1 (MQ=60)
                                                              TTAACAACCGAGACATTTACGGTCTAACAACCAGCAGATCTTCAGCCGCGCCGCTCCCCCGCCATTGGGGAACATACATTGATTCCACCATCGG                                <  SRR4302533.3162366/94‑1 (MQ=60)
                                                                AACAAGCGAGTCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTT                              <  SRR4302533.4965333/94‑1 (MQ=60)
                                                                AACAAGCGAGGCATTTACGCTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTT                              <  SRR4302533.360413/94‑1 (MQ=60)
                                                                 ACAAGCGAGTCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTG                             <  SRR4302533.2928487/94‑1 (MQ=60)
                                                                    AGCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGG                          <  SRR4302533.1806773/94‑1 (MQ=60)
                                                                     GCGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGT                         <  SRR4302533.2852391/94‑1 (MQ=60)
                                                                      CGAGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTA                        >  SRR4302533.1804106/1‑94 (MQ=60)
                                                                        AGGCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACC                      <  SRR4302533.1340451/94‑1 (MQ=60)
                                                                          GCATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTG                    >  SRR4302533.6334834/1‑94 (MQ=60)
                                                                           CATTTACGGTCTGACAACCGGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGA                   <  SRR4302533.4746887/94‑1 (MQ=60)
                                                                           CATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGA                   >  SRR4302533.2150632/1‑94 (MQ=60)
                                                                            ATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGAT                  >  SRR4302533.6170815/1‑94 (MQ=60)
                                                                            ATTTACGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGAT                  >  SRR4302533.1604688/1‑94 (MQ=60)
                                                                                 CGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTT             <  SRR4302533.5036725/94‑1 (MQ=60)
                                                                                 CGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTT             >  SRR4302533.4812692/1‑94 (MQ=60)
                                                                                 CGGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTT             >  SRR4302533.3310847/1‑94 (MQ=60)
                                                                                  GGTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTC            >  SRR4302533.1939175/1‑94 (MQ=60)
                                                                                   GTCTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCC           <  SRR4302533.3649623/94‑1 (MQ=60)
                                                                                     CTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCG         >  SRR4302533.3660363/1‑94 (MQ=60)
                                                                                     CTGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCG         >  SRR4302533.6126703/1‑94 (MQ=60)
                                                                                      TGACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGG        >  SRR4302533.3539096/1‑94 (MQ=60)
                                                                                       GACAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATGCATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGC       <  SRR4302533.3402881/94‑1 (MQ=60)
                                                                                       GACAACCAGCAGACCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGC       <  SRR4302533.2090143/94‑1 (MQ=60)
                                                                                         CAACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGT     >  SRR4302533.5198742/1‑94 (MQ=60)
                                                                                          AACCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTC    >  SRR4302533.2335850/1‑94 (MQ=60)
                                                                                            CCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCAC  >  SRR4302533.2390769/1‑94 (MQ=60)
                                                                                            |                                                                                             
CCACGCTGCAAGAAAAAGGAGAAAGGGCGCGGCTGCCAACGTTATCCAGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCAC  >  NC_000913/2645268‑2645453

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑