Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,631,832 (T)6→5 coding (259/1467 nt) guaB ← IMP dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,631,8270T.100.0% 1001.1 / NA 216coding (264/1467 nt)guaBIMP dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (105/111);  total (105/111)

Only 100 of 247 total aligned reads displayed.

AAAACCGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGTTTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAA  >  NC_000913/2631737‑2631915
                                                                                          |                                                                                        
 aaaCCGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGtttt                                                                                       <  1:2406090/93‑1 (MQ=255)
  aaCCGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGttttt                                                                                      >  2:85200/1‑93 (MQ=255)
    ccGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGtttttca                                                                                    <  2:634106/93‑3 (MQ=255)
    ccGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGGTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTgttgttca                                                                                    >  2:131628/1‑91 (MQ=255)
     cGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGtttttcac                                                                                   <  1:1488049/93‑4 (MQ=255)
      gTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGtttttcaca                                                                                  >  2:2352436/1‑89 (MQ=255)
      gTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGtttttcaca                                                                                  >  2:2206539/1‑89 (MQ=255)
      gTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGtttttcaca                                                                                  <  1:6242915/93‑5 (MQ=255)
       ttACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCacac                                                                                <  1:406102/93‑1 (MQ=255)
       ttACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCacac                                                                                <  2:346860/93‑1 (MQ=255)
        tACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACg                                                                               <  1:3217667/93‑1 (MQ=255)
         tcGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACgg                                                                              <  1:3919532/92‑1 (MQ=255)
          cGCTCTGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGc                                                                             <  1:2845205/93‑1 (MQ=255)
          cGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCCCCACACCAGATTCGTG‑TTTTTCACACGGc                                                                             <  1:5583254/93‑1 (MQ=255)
          cGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGc                                                                             <  2:2781658/93‑1 (MQ=255)
          cGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGc                                                                             >  1:1811061/1‑93 (MQ=255)
             tCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTGACCACACCAGATTCGTG‑TTTTTCACACGGCGaa                                                                          >  1:5113292/1‑93 (MQ=255)
               ggTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAAct                                                                        >  2:1832874/1‑93 (MQ=255)
                 tCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAActtc                                                                      >  2:3714929/1‑93 (MQ=255)
                    gTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCCGATTCGTG‑TTTTTCACACGGCGAACttcttc                                                                   >  2:2853065/1‑93 (MQ=255)
                    gTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACttcttc                                                                   >  2:1521208/1‑93 (MQ=255)
                    gTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACttcttc                                                                   >  2:2441823/1‑93 (MQ=255)
                    gTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACttcttc                                                                   >  2:3139086/1‑93 (MQ=255)
                    gTTATTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACttcttc                                                                   <  2:1658739/93‑1 (MQ=255)
                      tCTTTCACTTCGCGCAGCGGCGTGGTATTCACAGCAGTCTTCGGATCAGTTACCACACCAGGTTCGTG‑ATTTTCACACGGCGAACTtgttcta                                                                 >  2:3848076/1‑92 (MQ=255)
                       cTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGc                                                                >  2:4161136/1‑93 (MQ=255)
                       cTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGc                                                                >  2:6198946/1‑93 (MQ=255)
                        tttCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGcc                                                               >  2:5082364/1‑93 (MQ=255)
                        tttCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGcc                                                               <  2:76415/93‑1 (MQ=255)
                        tttCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGcc                                                               <  1:4253448/93‑1 (MQ=255)
                        tttCACTACGCGCAGCGTCGTGGTTGGCAGACCAGTCTGCGGATCAGTCACCGCACCAGATTCGTG‑TTTTTCACGCGGCGAACTTCTTCTggc                                                               <  2:3320589/93‑3 (MQ=255)
                          tCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTg                                                             <  1:811717/93‑1 (MQ=255)
                            aCTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGc                                                           >  1:4755137/1‑93 (MQ=255)
                            aCTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGc                                                           <  1:3281702/93‑1 (MQ=255)
                             cTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCg                                                          <  1:1418873/93‑1 (MQ=255)
                              ttCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGt                                                         <  2:6039241/93‑1 (MQ=255)
                              ttCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGt                                                         >  1:3789892/1‑93 (MQ=255)
                               tCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGtt                                                        <  1:3847640/93‑1 (MQ=255)
                                cgcgCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTc                                                       <  2:1047023/93‑1 (MQ=255)
                                  cgcAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCaa                                                     <  2:2654637/93‑1 (MQ=255)
                                  cgcAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCaa                                                     <  2:2571513/93‑1 (MQ=255)
                                   gcAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAAt                                                    >  1:5129019/1‑93 (MQ=255)
                                   gcAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAAt                                                    >  2:5165643/1‑93 (MQ=255)
                                    cAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATg                                                   >  2:341469/1‑93 (MQ=255)
                                    cAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATg                                                   <  1:852947/93‑1 (MQ=255)
                                      gCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGa                                                 >  2:1739119/1‑93 (MQ=255)
                                       cgtcgtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGAc                                                <  2:5952691/93‑1 (MQ=255)
                                       cgtcgtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGAc                                                <  2:4600062/93‑1 (MQ=255)
                                        gtcgtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACa                                               <  1:4916823/93‑1 (MQ=255)
                                        gtcgtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACa                                               >  1:4456266/1‑93 (MQ=255)
                                         tcgtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACAt                                              >  1:530901/1‑93 (MQ=255)
                                         tcgtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACAt                                              >  1:5992740/1‑93 (MQ=255)
                                         tcgtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACAt                                              <  2:5564995/93‑1 (MQ=255)
                                          cgtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATg                                             <  1:884459/93‑1 (MQ=255)
                                          cgtGGTTGGCAGAACAGTCTGCGAATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATg                                             <  1:254532/93‑1 (MQ=255)
                                           gtGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGt                                            <  2:4832595/93‑1 (MQ=255)
                                            tGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGtt                                           >  1:5170180/1‑93 (MQ=255)
                                             ggTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGttt                                          <  2:1560698/93‑1 (MQ=255)
                                               ttGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGttttt                                        <  2:3008409/93‑1 (MQ=255)
                                                tGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTtg                                       <  2:4933261/93‑1 (MQ=255)
                                                 ggCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTtgt                                      <  1:1976657/93‑1 (MQ=255)
                                                 ggCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTtgt                                      <  2:3583043/93‑1 (MQ=255)
                                                 ggCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTtgt                                      <  1:5622588/93‑1 (MQ=255)
                                                   cAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTgg                                    >  1:2013478/1‑93 (MQ=255)
                                                   cAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTgg                                    <  2:1178013/93‑1 (MQ=255)
                                                    aGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGa                                   <  2:5058340/93‑1 (MQ=255)
                                                    aGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGa                                   <  2:5754594/93‑1 (MQ=255)
                                                     gAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGTGTTCAATGGACATGTTTTTGTGGAt                                  >  1:3133592/1‑93 (MQ=255)
                                                     gAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGAt                                  >  2:441893/1‑93 (MQ=255)
                                                     gAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGAt                                  <  2:694513/93‑1 (MQ=255)
                                                     gAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGAt                                  >  1:1619420/1‑93 (MQ=255)
                                                      aaCAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATa                                 <  1:2773531/93‑1 (MQ=255)
                                                      aaCAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATa                                 >  2:5915113/1‑93 (MQ=255)
                                                       aCAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATaa                                >  2:3070330/1‑93 (MQ=255)
                                                       aCAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATaa                                >  1:1376876/1‑93 (MQ=255)
                                                        cAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATaaa                               <  2:386946/93‑1 (MQ=255)
                                                        cAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATaaa                               <  2:3976603/93‑1 (MQ=255)
                                                        cAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATaaa                               >  2:558378/1‑93 (MQ=255)
                                                        cAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATaaa                               >  2:3234100/1‑93 (MQ=255)
                                                           tCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTTGATAAAGcc                            >  2:5422677/1‑93 (MQ=255)
                                                               cGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATa                        <  1:3579351/93‑1 (MQ=255)
                                                               cGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATa                        >  2:5264435/1‑93 (MQ=255)
                                                               cGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATa                        >  1:5830949/1‑93 (MQ=255)
                                                                ggATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATAc                       >  1:6159082/1‑93 (MQ=255)
                                                                 gATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATAcc                      <  1:1105421/93‑1 (MQ=255)
                                                                 gATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATAcc                      >  2:2239106/1‑93 (MQ=255)
                                                                        caccacaccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTcc               >  1:4387873/1‑93 (MQ=255)
                                                                        caccacaccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTcc               <  1:1062471/93‑1 (MQ=255)
                                                                         accacaccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCt              >  1:2874936/1‑93 (MQ=255)
                                                                          ccacaccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTg             <  1:1279574/93‑1 (MQ=255)
                                                                          ccacaccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTg             <  2:602192/93‑1 (MQ=255)
                                                                           cacaccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGa            <  1:2982654/93‑1 (MQ=255)
                                                                           cacaccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGa            <  2:1579144/93‑1 (MQ=255)
                                                                            acaccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCGTTCCTGAg           >  2:4516375/1‑93 (MQ=255)
                                                                             caccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGc          <  1:5352269/93‑1 (MQ=255)
                                                                               ccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCa        <  2:2002205/93‑1 (MQ=255)
                                                                               ccaGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACAGCCTTCCTGAGCCa        >  1:2111422/1‑93 (MQ=255)
                                                                                caGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCag       >  1:5480830/1‑93 (MQ=255)
                                                                                 aGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCaga      >  1:5677011/1‑93 (MQ=255)
                                                                                   aTTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGc    <  2:4037448/93‑1 (MQ=255)
                                                                                          |                                                                                        
AAAACCGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGTTTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAA  >  NC_000913/2631737‑2631915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGTTTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAGCCAGGCG  >  NC_000913/2631741‑2631925
                                                                                           |                                                                                             
CCGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCAC                                                                                            <  SRR4302533.3238637/94‑1 (MQ=60)
 CGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACA                                                                                           >  SRR4302533.4420408/1‑94 (MQ=60)
 CGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACA                                                                                           <  SRR4302533.1497026/94‑1 (MQ=60)
 CGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACA                                                                                           <  SRR4302533.1331644/94‑1 (MQ=60)
  GTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACAC                                                                                          <  SRR4302533.5727417/94‑1 (MQ=60)
  GTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACAC                                                                                          <  SRR4302533.1349011/94‑1 (MQ=60)
  GTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACAC                                                                                          <  SRR4302533.6280286/94‑1 (MQ=60)
  GTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACAC                                                                                          >  SRR4302533.2723438/1‑94 (MQ=60)
  CTTACGCTCGGTCAGTTCTTTCCCTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACAC                                                                                          <  SRR4302533.322782/94‑1 (MQ=60)
   TTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACG                                                                                         <  SRR4302533.5679020/94‑1 (MQ=60)
   TTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACG                                                                                         <  SRR4302533.408542/94‑1 (MQ=60)
    TACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGG                                                                                        <  SRR4302533.3237508/94‑1 (MQ=60)
     TCGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGC                                                                                       <  SRR4302533.3943469/94‑1 (MQ=60)
     ACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGC                                                                                       >  SRR4302533.1822125/1‑94 (MQ=60)
      CGCTCTGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCG                                                                                      <  SRR4302533.2862716/94‑1 (MQ=60)
      CGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCCCCACACCAGATTCGTG‑TTTTTCACACGGCG                                                                                      <  SRR4302533.5616989/94‑1 (MQ=60)
      CGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCG                                                                                      >  SRR4302533.4385055/1‑94 (MQ=60)
        CTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTGACCACACCAGATTCGTG‑TTTTTCACACGGCGAA                                                                                    >  SRR4302533.5144191/1‑94 (MQ=60)
        CTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAA                                                                                    >  SRR4302533.4789832/1‑94 (MQ=60)
        CTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACCGCGAA                                                                                    >  SRR4302533.2381745/1‑94 (MQ=60)
         TCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAAC                                                                                   >  SRR4302533.5519252/1‑94 (MQ=60)
          CGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACT                                                                                  <  SRR4302533.3835052/94‑1 (MQ=60)
           GGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTT                                                                                 >  SRR4302533.5976599/1‑94 (MQ=60)
             TCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCT                                                                               <  SRR4302533.4723398/94‑1 (MQ=60)
             TCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCT                                                                               <  SRR4302533.4354090/94‑1 (MQ=60)
             TCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCT                                                                               <  SRR4302533.818625/94‑1 (MQ=60)
              CAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTT                                                                              >  SRR4302533.4581800/1‑94 (MQ=60)
                 TTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTATTCTG                                                                           >  SRR4302533.2133853/1‑94 (MQ=60)
                  TCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGC                                                                          >  SRR4302533.5314853/1‑94 (MQ=60)
                  TCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGC                                                                          >  SRR4302533.2429939/1‑94 (MQ=60)
                    TTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCTGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCT                                                                        <  SRR4302533.889217/94‑1 (MQ=60)
                    TTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCT                                                                        >  SRR4302533.4065868/1‑94 (MQ=60)
                    TTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCT                                                                        <  SRR4302533.4279102/94‑1 (MQ=60)
                     TTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTG                                                                       <  SRR4302533.3650233/94‑1 (MQ=60)
                      TCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGG                                                                      <  SRR4302533.816473/94‑1 (MQ=60)
                       CACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGC                                                                     >  SRR4302533.4783992/1‑94 (MQ=60)
                        ACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCG                                                                    <  SRR4302533.3301963/94‑1 (MQ=60)
                         CTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGT                                                                   >  SRR4302533.3813098/1‑94 (MQ=60)
                         CTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGT                                                                   <  SRR4302533.1427458/94‑1 (MQ=60)
                           TCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTC                                                                 <  SRR4302533.3871134/94‑1 (MQ=60)
                             GCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAA                                                               >  SRR4302533.875039/1‑94 (MQ=60)
                              CGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAAT                                                              >  SRR4302533.5150998/1‑94 (MQ=60)
                              CGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAAT                                                              >  SRR4302533.5160001/1‑94 (MQ=60)
                               GCAGCGTCGTGGTTGGCAGAACAGTCTGCTGATCAGTCGCCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATG                                                             <  SRR4302533.6310477/94‑1 (MQ=60)
                                CAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGG                                                            <  SRR4302533.857940/94‑1 (MQ=60)
                                 AGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGA                                                           <  SRR4302533.5050652/94‑1 (MQ=60)
                                 AGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGA                                                           >  SRR4302533.3326144/1‑94 (MQ=60)
                                 AGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGA                                                           >  SRR4302533.1567947/1‑94 (MQ=60)
                                   CGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACA                                                         >  SRR4302533.1456301/1‑94 (MQ=60)
                                   CGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACA                                                         >  SRR4302533.4483214/1‑94 (MQ=60)
                                    GTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACAT                                                        >  SRR4302533.140129/1‑94 (MQ=60)
                                    GTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACAT                                                        >  SRR4302533.607801/1‑94 (MQ=60)
                                    GTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACAT                                                        >  SRR4302533.1665375/1‑94 (MQ=60)
                                    GTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACAT                                                        >  SRR4302533.6029041/1‑94 (MQ=60)
                                    GTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACAT                                                        >  SRR4302533.534059/1‑94 (MQ=60)
                                    GTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACAT                                                        <  SRR4302533.4946604/94‑1 (MQ=60)
                                      CGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGT                                                      <  SRR4302533.889655/94‑1 (MQ=60)
                                      CGTGGTTGGCAGAACAGTCTGCGAATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGT                                                      <  SRR4302533.256091/94‑1 (MQ=60)
                                       GTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTT                                                     >  SRR4302533.5201383/1‑94 (MQ=60)
                                       GTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTT                                                     <  SRR4302533.3104214/94‑1 (MQ=60)
                                        TGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTT                                                    <  SRR4302533.38243/94‑1 (MQ=60)
                                             GGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTG                                               <  SRR4302533.1988763/94‑1 (MQ=60)
                                             GGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTG                                               >  SRR4302533.1995001/1‑94 (MQ=60)
                                             GGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTG                                               <  SRR4302533.5656572/94‑1 (MQ=60)
                                              GCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGG                                              >  SRR4302533.2025884/1‑94 (MQ=60)
                                               CAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGA                                             >  SRR4302533.5472698/1‑94 (MQ=60)
                                                AGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGTGTTCAATGGACATGTTTTTGTGGAT                                            >  SRR4302533.3152859/1‑94 (MQ=60)
                                                AGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGAT                                            >  SRR4302533.1629229/1‑94 (MQ=60)
                                                  AACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAA                                          >  SRR4302533.1385160/1‑94 (MQ=60)
                                                  AACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAA                                          <  SRR4302533.2790558/94‑1 (MQ=60)
                                                  AACAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAA                                          >  SRR4302533.6250054/1‑94 (MQ=60)
                                                    CAGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAG                                        <  SRR4302533.3226422/94‑1 (MQ=60)
                                                     AGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGC                                       <  SRR4302533.2866162/94‑1 (MQ=60)
                                                     AGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGC                                       >  SRR4302533.1714122/1‑94 (MQ=60)
                                                     AGTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCCGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGC                                       >  SRR4302533.3350548/1‑94 (MQ=60)
                                                      GTCTGCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCC                                      <  SRR4302533.5832561/94‑1 (MQ=60)
                                                          GCGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATA                                  >  SRR4302533.5866192/1‑94 (MQ=60)
                                                           CGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATAC                                 >  SRR4302533.6196126/1‑94 (MQ=60)
                                                           CGGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATAC                                 <  SRR4302533.3601407/94‑1 (MQ=60)
                                                            GGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACC                                >  SRR4302533.293744/1‑94 (MQ=60)
                                                            GGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACC                                <  SRR4302533.5350714/94‑1 (MQ=60)
                                                            GGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACC                                >  SRR4302533.4961269/1‑94 (MQ=60)
                                                            GGATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACC                                <  SRR4302533.5617900/94‑1 (MQ=60)
                                                             GATCAGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCG                               <  SRR4302533.1111974/94‑1 (MQ=60)
                                                                 AGTCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTT                           <  SRR4302533.4080252/94‑1 (MQ=60)
                                                                   TCACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCC                         >  SRR4302533.4414363/1‑94 (MQ=60)
                                                                    CACTACACCAGATTCGTG‑TTTTTCACACGGCGTACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCT                        <  SRR4302533.5196836/94‑1 (MQ=60)
                                                                    CACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCT                        >  SRR4302533.2892624/1‑94 (MQ=60)
                                                                    CACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCT                        <  SRR4302533.5196835/94‑1 (MQ=60)
                                                                    CACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCT                        >  SRR4302533.2892625/1‑94 (MQ=60)
                                                                    CACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCT                        <  SRR4302533.711534/94‑1 (MQ=60)
                                                                    CACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCT                        >  SRR4302533.109813/1‑94 (MQ=60)
                                                                    CACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCT                        <  SRR4302533.1068715/94‑1 (MQ=60)
                                                                     ACCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTG                       >  SRR4302533.3223720/1‑94 (MQ=60)
                                                                      CCACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGA                      <  SRR4302533.1287266/94‑1 (MQ=60)
                                                                       CACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAG                     <  SRR4302533.1961549/94‑1 (MQ=60)
                                                                       CACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAG                     <  SRR4302533.2099857/94‑1 (MQ=60)
                                                                       CACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAG                     <  SRR4302533.5391839/94‑1 (MQ=60)
                                                                       CACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAG                     <  SRR4302533.3000898/94‑1 (MQ=60)
                                                                       CACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAG                     <  SRR4302533.2016422/94‑1 (MQ=60)
                                                                        ACACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGC                    <  SRR4302533.3897538/94‑1 (MQ=60)
                                                                         CACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCC                   >  SRR4302533.4117692/1‑94 (MQ=60)
                                                                         CACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCC                   <  SRR4302533.5384387/94‑1 (MQ=60)
                                                                          ACCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACAGCCTTCCTGAGCCA                  >  SRR4302533.2124445/1‑94 (MQ=60)
                                                                           CCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAG                 >  SRR4302533.5513873/1‑94 (MQ=60)
                                                                           CCAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAG                 >  SRR4302533.1495495/1‑94 (MQ=60)
                                                                            CAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGA                <  SRR4302533.2599850/94‑1 (MQ=60)
                                                                            CAGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGA                >  SRR4302533.5711296/1‑94 (MQ=60)
                                                                             AGATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAG               <  SRR4302533.113457/94‑1 (MQ=60)
                                                                              GATTCGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGC              >  SRR4302533.5652995/1‑94 (MQ=60)
                                                                                  CGTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATA          >  SRR4302533.2337463/1‑94 (MQ=60)
                                                                                   GTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAG         <  SRR4302533.5997317/94‑1 (MQ=60)
                                                                                   GTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAG         <  SRR4302533.2182311/94‑1 (MQ=60)
                                                                                   GTG‑TTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAG         <  SRR4302533.1444695/94‑1 (MQ=60)
                                                                                     tgTTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAGC        >  SRR4302533.3948155/3‑94 (MQ=60)
                                                                                     tgTTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAGC        >  SRR4302533.4147207/3‑94 (MQ=60)
                                                                                        TTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAGCCAG     <  SRR4302533.4498700/94‑1 (MQ=60)
                                                                                        TTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAGCCAG     <  SRR4302533.4151414/94‑1 (MQ=60)
                                                                                        TTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAGCCAG     >  SRR4302533.2048779/1‑94 (MQ=60)
                                                                                         TTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAGCCAGG    >  SRR4302533.4368912/1‑94 (MQ=60)
                                                                                           TCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAGCCAGGCG  <  SRR4302533.3727155/94‑1 (MQ=60)
                                                                                           |                                                                                             
CCGTTACGCTCGGTCAGTTCTTTCACTTCGCGCAGCGTCGTGGTTGGCAGAACAGTCTGCGGATCAGTCACCACACCAGATTCGTGTTTTTTCACACGGCGAACTTCTTCTGCCTGGCGTTCAATGGACATGTTTTTGTGGATAAAGCCGATACCGCCTTCCTGAGCCAGAGCAATAGCCAGGCG  >  NC_000913/2631741‑2631925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑