Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,661,865	T→A	L135Q (CTG→CAG)	suhB →	SuhB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,661,865	0	T	A	100.0%	908.5 / NA	247	L135Q (CTG→CAG)	suhB	SuhB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (118/129); total (118/129)

Only 100 of 256 total aligned reads displayed.

TTCGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTAC  >  NC_000913/2661775‑2661953

 tCGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGaa                                                                                       <  2:10009648/93‑1 (MQ=255)

 tCGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGaa                                                                                       <  2:3491269/93‑1 (MQ=255)

 tCGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGaa                                                                                       <  2:797866/93‑1 (MQ=255)

   gTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACg                                                                                     >  1:9512240/1‑93 (MQ=255)

   gTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACg                                                                                     >  2:8893937/1‑93 (MQ=255)

      tCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCt                                                                                  >  2:4127819/1‑93 (MQ=255)

      tCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCt                                                                                  >  1:5124656/1‑93 (MQ=255)

      tCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCt                                                                                  >  1:2921122/1‑93 (MQ=255)

       cAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCtc                                                                                 >  2:5425133/1‑92 (MQ=255)

        aaaGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTAc                                                                                <  1:9139333/93‑1 (MQ=255)

        aaaGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTAc                                                                                <  2:2048151/93‑1 (MQ=255)

         aaGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTAcc                                                                               <  2:8345777/93‑1 (MQ=255)

          aGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCg                                                                              >  2:3210259/1‑93 (MQ=255)

          aGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCg                                                                              >  2:6991481/1‑93 (MQ=255)

           ggCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGa                                                                             <  1:7021552/93‑1 (MQ=255)

            gCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGAc                                                                            <  2:2624273/93‑1 (MQ=255)

               gCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTgc                                                                         >  2:6717111/1‑93 (MQ=255)

               gCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTgc                                                                         <  2:6329877/93‑1 (MQ=255)

               gCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTgc                                                                         <  1:5843380/93‑1 (MQ=255)

                cACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGCCTgcg                                                                        >  2:4924139/1‑93 (MQ=255)

                cACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTgcg                                                                        >  2:6410244/1‑93 (MQ=255)

                cACCGAAGTTGCTGTGGTATACGATCCTATGCGGAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTgcg                                                                        <  2:461343/93‑1 (MQ=255)

                 aCCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTgcgc                                                                       >  2:1300357/1‑93 (MQ=255)

                  ccGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGcgcg                                                                      >  2:8568539/1‑93 (MQ=255)

                  ccGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGcgcg                                                                      >  1:1862511/1‑93 (MQ=255)

                    gAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGgc                                                                    <  1:1397766/93‑1 (MQ=255)

                    gAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGgc                                                                    >  1:7804140/1‑93 (MQ=255)

                    gAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGgc                                                                    <  1:8639711/93‑1 (MQ=255)

                    gAAGTTGCTGGGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGgc                                                                    <  1:3464970/93‑1 (MQ=255)

                     aaGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGgca                                                                   >  2:9847221/1‑93 (MQ=255)

                     aaGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGgca                                                                   <  1:3382364/93‑1 (MQ=255)

                     aaGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGgca                                                                   <  2:8291322/93‑1 (MQ=255)

                      aGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGgcag                                                                  <  1:7484406/93‑1 (MQ=255)

                       gTTGCTGTGGTATACGAGCCTTTGCGTAACGAACTGTTCCCCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTGCCGACTGCGCGgcagc                                                                 <  1:3268503/93‑1 (MQ=255)

                        ttGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGgcagca                                                                >  2:3366550/1‑93 (MQ=255)

                         tGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCAc                                                               <  2:3900944/93‑1 (MQ=255)

                            tgtgGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGc                                                            >  2:2364864/1‑93 (MQ=255)

                            tgtgGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGc                                                            >  2:4387733/1‑93 (MQ=255)

                                gTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTcgc                                                        <  1:1119618/93‑1 (MQ=255)

                                  ataCGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGa                                                      >  1:8260035/1‑93 (MQ=255)

                                   tccGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGGCTGCGCGGCAGCACCGCTCGCGAt                                                     <  1:7260388/91‑1 (MQ=255)

                                   taCCATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGAt                                                     <  2:7246532/93‑1 (MQ=255)

                                    aCGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGGGCGGCAGCACCGCTCGCGAtc                                                    >  1:9182507/1‑93 (MQ=255)

                                    aCGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGAtc                                                    <  1:2413865/93‑1 (MQ=255)

                                      gATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGAtctc                                                  <  1:4520848/93‑1 (MQ=255)

                                      gATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGAtctc                                                  <  2:1695163/93‑1 (MQ=255)

                                       aTCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCg                                                 >  2:9537755/1‑93 (MQ=255)

                                        accTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGa                                                <  2:8530515/92‑1 (MQ=255)

                                         ccTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGAc                                               >  1:3690903/1‑93 (MQ=255)

                                          cTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACg                                              >  1:9090956/1‑93 (MQ=255)

                                          cTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACg                                              >  2:2859514/1‑93 (MQ=255)

                                           tATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACgg                                             >  1:6710076/1‑93 (MQ=255)

                                           tATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACgg                                             <  1:6968469/93‑1 (MQ=255)

                                            aTGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGt                                            >  2:1204175/1‑93 (MQ=255)

                                            aTGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGt                                            >  1:964540/1‑93 (MQ=255)

                                             tGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTa                                           >  1:7734793/1‑93 (MQ=255)

                                             tGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTa                                           >  2:8194575/1‑93 (MQ=255)

                                             tGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTa                                           >  1:6091970/1‑93 (MQ=255)

                                                gTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGGACTa                                        >  2:2237226/1‑93 (MQ=255)

                                                 tAACGACCTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTAt                                       <  2:7680840/93‑1 (MQ=255)

                                                 tAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTAt                                       <  1:73818/93‑1 (MQ=255)

                                                  aaCGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTAtt                                      <  1:8007618/93‑1 (MQ=255)

                                                   aCGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTc                                     <  2:3045281/93‑1 (MQ=255)

                                                   aCGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTc                                     <  2:9707703/93‑1 (MQ=255)

                                                    cGAACTGTTCGCCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCt                                    <  2:3060336/93‑1 (MQ=255)

                                                    cGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCt                                    >  2:1539164/1‑93 (MQ=255)

                                                    cGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCt                                    <  1:8898065/93‑1 (MQ=255)

                                                    cGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCt                                    <  1:7120014/93‑1 (MQ=255)

                                                    cGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCt                                    >  2:4242602/1‑93 (MQ=255)

                                                     gAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTg                                   >  2:6942335/1‑93 (MQ=255)

                                                     gAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTg                                   <  2:8741666/93‑1 (MQ=255)

                                                          gTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGAc                              >  1:1381857/1‑93 (MQ=255)

                                                          gTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGAc                              >  2:7274052/1‑93 (MQ=255)

                                                          gTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGGACTATTCTGGCGAc                              >  1:8921197/1‑93 (MQ=255)

                                                           ttCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGAcc                             >  2:5262490/1‑93 (MQ=255)

                                                           ttCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGAcc                             >  2:7375782/1‑93 (MQ=255)

                                                              cccGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGc                          <  2:7775193/92‑1 (MQ=255)

                                                                   cACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTccc                     >  1:2624676/1‑93 (MQ=255)

                                                                    aCTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCg                    >  1:3995634/1‑93 (MQ=255)

                                                                     cTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGt                   >  1:6380950/1‑93 (MQ=255)

                                                                     cTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGt                   >  2:6678445/1‑93 (MQ=255)

                                                                     cTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGt                   >  2:1698737/1‑93 (MQ=255)

                                                                      tCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGtt                  >  2:8909746/1‑93 (MQ=255)

                                                                      tCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCCGCTTCCCGtt                  >  2:401368/1‑93 (MQ=255)

                                                                       cgcgGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTc                 >  1:3503480/1‑93 (MQ=255)

                                                                         cgGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCaa               <  2:5897586/93‑1 (MQ=255)

                                                                         cgGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCCGCTTCCCGTTCaa               >  1:8081705/1‑93 (MQ=255)

                                                                          ggTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGAGACCGGCTTCCCGTTCaaa              >  1:3061790/1‑93 (MQ=255)

                                                                             cAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGCTCAAAGCa           >  1:10002900/1‑93 (MQ=255)

                                                                               gggCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCaaa         <  2:2526267/93‑1 (MQ=255)

                                                                                ggCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCaaaa        >  1:4147933/1‑93 (MQ=255)

                                                                                  cgcACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACa      <  1:1658577/93‑1 (MQ=255)

                                                                                    cacaGCAGAACGGCTACCGGCTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGt    <  1:2812671/93‑1 (MQ=255)

                                                                                    cacaGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGt    <  2:5060309/93‑1 (MQ=255)

                                                                                    cacaGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGt    <  2:9318246/93‑1 (MQ=255)

                                                                                    cacaGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGt    <  2:330010/93‑1 (MQ=255)

                                                                                     acaGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTa   <  1:5713936/93‑1 (MQ=255)

                                                                                     acaGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGGTCAAAGCAAAACAGTa   >  1:7073587/1‑93 (MQ=255)

                                                                                      cagcagAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTAc  >  2:9096550/1‑93 (MQ=255)

                                                                                      cagcagAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTAc  >  1:9915664/1‑93 (MQ=255)

TTCGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTAC  >  NC_000913/2661775‑2661953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTGTTCGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTACGCCAC  >  NC_000913/2661772‑2661958

GTGTTCGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCA                                                                                               <  SRR4302540.290519/94‑1 (MQ=60)

CTGTTCGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCA                                                                                               <  SRR4302540.6702156/94‑1 (MQ=60)

 TGTTCGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAG                                                                                              <  SRR4302540.787725/94‑1 (MQ=60)

 TGTTCGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAG                                                                                              <  SRR4302540.4034113/94‑1 (MQ=60)

   TTCGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAA                                                                                            <  SRR4302540.7845619/94‑1 (MQ=60)

    TCGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAAC                                                                                           <  SRR4302540.6480806/94‑1 (MQ=60)

    TCGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAAC                                                                                           <  SRR4302540.1182582/94‑1 (MQ=60)

    TCGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAAC                                                                                           <  SRR4302540.7131233/94‑1 (MQ=60)

    TCGTACCAAAGGCCGCACCGAAGTTGCTGCGGTATACGATCCTATGCGAAACGAACTGTTCACCGCCCCTCGCGGTCAGGGCGCACAGCAGAAC                                                                                           <  SRR4302540.9174925/94‑1 (MQ=60)

     CGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACG                                                                                          >  SRR4302540.9624911/1‑94 (MQ=60)

      GTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGG                                                                                         >  SRR4302540.5902862/1‑94 (MQ=60)

        ATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCT                                                                                       >  SRR4302540.5180162/1‑94 (MQ=60)

        ATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCT                                                                                       >  SRR4302540.1237884/1‑94 (MQ=60)

        ATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCT                                                                                       >  SRR4302540.9278403/1‑94 (MQ=60)

        ATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCT                                                                                       >  SRR4302540.2953131/1‑94 (MQ=60)

          CAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTAC                                                                                     <  SRR4302540.8199762/94‑1 (MQ=60)

           AAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACC                                                                                    <  SRR4302540.9249137/94‑1 (MQ=60)

            AAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCG                                                                                   <  SRR4302540.3136619/94‑1 (MQ=60)

              GGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGAC                                                                                 <  SRR4302540.7099539/94‑1 (MQ=60)

               GCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACT                                                                                <  SRR4302540.2743159/94‑1 (MQ=60)

               GCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACT                                                                                <  SRR4302540.5520491/94‑1 (MQ=60)

                CCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTG                                                                               <  SRR4302540.763922/94‑1 (MQ=60)

                 CGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGC                                                                              <  SRR4302540.9347693/94‑1 (MQ=60)

                  GCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCG                                                                             <  SRR4302540.5908006/94‑1 (MQ=60)

                   CACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGC                                                                            >  SRR4302540.1464100/1‑94 (MQ=60)

                    ACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCG                                                                           >  SRR4302540.1882931/1‑94 (MQ=60)

                    ACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCG                                                                           >  SRR4302540.5625141/1‑94 (MQ=60)

                      CGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGC                                                                         >  SRR4302540.7895343/1‑94 (MQ=60)

                       GAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCA                                                                        <  SRR4302540.10163377/94‑1 (MQ=60)

                       GAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCA                                                                        <  SRR4302540.8740069/94‑1 (MQ=60)

                       GAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCA                                                                        <  SRR4302540.1412778/94‑1 (MQ=60)

                       GAAGTTGCTGGGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCA                                                                        <  SRR4302540.3502687/94‑1 (MQ=60)

                        AAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAG                                                                       <  SRR4302540.3419283/94‑1 (MQ=60)

                         AGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGC                                                                      >  SRR4302540.4070225/1‑94 (MQ=60)

                         AGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGC                                                                      <  SRR4302540.7567949/94‑1 (MQ=60)

                          GTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCA                                                                     <  SRR4302540.3304176/94‑1 (MQ=60)

                          GTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTAACGACTGCGCGGCAtaa                                                                     >  SRR4302540.814790/1‑91 (MQ=60)

                          GTTGCTGTGGTATACGAGCCTTTGCGTAACGAACTGTTCCCCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTGCCGACTGCGCGGCAGCA                                                                     <  SRR4302540.3304175/94‑1 (MQ=60)

                           TTGCGGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCAC                                                                    <  SRR4302540.7208475/94‑1 (MQ=60)

                            TGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACC                                                                   <  SRR4302540.3928257/94‑1 (MQ=60)

                            TGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACC                                                                   <  SRR4302540.345977/94‑1 (MQ=60)

                             ACTGTGGTAGAAGATCCTATGCGTAACGAACTGATCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCG                                                                  <  SRR4302540.3150093/94‑1 (MQ=60)

                               TGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCT                                                                >  SRR4302540.4580116/1‑94 (MQ=60)

                                 TGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCG                                                              >  SRR4302540.3300668/1‑94 (MQ=60)

                                   GTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCG                                                            <  SRR4302540.1131232/94‑1 (MQ=60)

                                    TATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGA                                                           >  SRR4302540.8356022/1‑94 (MQ=60)

                                     ATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGAT                                                          >  SRR4302540.795306/1‑94 (MQ=60)

                                      TCCGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGGCTGCGCGGCAGCACCGCTCGCGATC                                                         <  SRR4302540.7341401/94‑1 (MQ=60)

                                      TACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGGGCGGCAGCACCGCTCGCGATC                                                         >  SRR4302540.9292634/1‑94 (MQ=60)

                                      TACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATC                                                         >  SRR4302540.2020257/1‑94 (MQ=60)

                                      TACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATC                                                         >  SRR4302540.1424664/1‑94 (MQ=60)

                                      TACGATCCTATGCGTAACGAACTGTTCACCGCAACTCGCGGTCAGGGACCCAAGCAGGACGGCTATCGACTGCGCGGCAGCAGCGCTCGCGATC                                                         >  SRR4302540.1424663/1‑94 (MQ=53)

                                       ACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTGCCGACTGCGCGGCAGCACCGCTCGCGATCT                                                        <  SRR4302540.10015383/94‑1 (MQ=60)

                                       ACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCT                                                        <  SRR4302540.2439881/94‑1 (MQ=60)

                                         GATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCG                                                      <  SRR4302540.4569810/94‑1 (MQ=60)

                                         GATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCG                                                      <  SRR4302540.2865487/94‑1 (MQ=60)

                                         GATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCG                                                      >  SRR4302540.8397405/1‑94 (MQ=60)

                                          ATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGA                                                     >  SRR4302540.6289159/1‑94 (MQ=60)

                                           TCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGAC                                                    >  SRR4302540.9122227/1‑94 (MQ=60)

                                           TCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGAC                                                    >  SRR4302540.2879945/1‑94 (MQ=60)

                                           TCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGAC                                                    >  SRR4302540.3731214/1‑94 (MQ=60)

                                           TCCTATGCGGAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGAC                                                    <  SRR4302540.3630305/94‑1 (MQ=60)

                                            CCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACG                                                   >  SRR4302540.9200173/1‑94 (MQ=60)

                                            CCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACG                                                   <  SRR4302540.8314161/94‑1 (MQ=60)

                                             CTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGG                                                  >  SRR4302540.6784120/1‑94 (MQ=60)

                                              TATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGT                                                 <  SRR4302540.7045938/94‑1 (MQ=60)

                                              TATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGT                                                 >  SRR4302540.974500/1‑94 (MQ=60)

                                              TATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGT                                                 >  SRR4302540.8831155/1‑94 (MQ=60)

                                               ATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTA                                                >  SRR4302540.7825093/1‑94 (MQ=60)

                                               ATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTA                                                >  SRR4302540.6159772/1‑94 (MQ=60)

                                                  CGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTA                                             <  SRR4302540.4538600/94‑1 (MQ=60)

                                                  CGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTA                                             >  SRR4302540.4374407/1‑94 (MQ=60)

                                                   GTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTAT                                            >  SRR4302540.2500818/1‑94 (MQ=60)

                                                   GTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTAT                                            >  SRR4302540.3468355/1‑94 (MQ=60)

                                                   GTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTAT                                            >  SRR4302540.3510001/1‑94 (MQ=60)

                                                    TAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATT                                           <  SRR4302540.74585/94‑1 (MQ=60)

                                                    TAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTCGGCGGCAGCACCTCTCGCGTTCGCGTCTGTACTATT                                           >  SRR4302540.5179572/1‑94 (MQ=60)

                                                     AACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTC                                          >  SRR4302540.6465753/1‑94 (MQ=60)

                                                     AACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTC                                          <  SRR4302540.8100793/94‑1 (MQ=60)

                                                      ACGAACTGTTCGCCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCT                                         <  SRR4302540.9688243/94‑1 (MQ=60)

                                                      ACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCT                                         <  SRR4302540.10050696/94‑1 (MQ=60)

                                                       CGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTG                                        <  SRR4302540.140242/94‑1 (MQ=60)

                                                       CGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTG                                        <  SRR4302540.3635004/94‑1 (MQ=60)

                                                       CGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTG                                        <  SRR4302540.7199292/94‑1 (MQ=60)

                                                       CGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTG                                        <  SRR4302540.9001072/94‑1 (MQ=60)

                                                       CGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAAAGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTG                                        >  SRR4302540.2352053/1‑94 (MQ=60)

                                                          ACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCG                                     <  SRR4302540.7945475/94‑1 (MQ=60)

                                                            TGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGAC                                   >  SRR4302540.1396608/1‑94 (MQ=60)

                                                            TGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGAC                                   >  SRR4302540.7169899/1‑94 (MQ=60)

                                                            TGTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGGACTATTCTGGCGAC                                   >  SRR4302540.9024613/1‑94 (MQ=60)

                                                             GTTCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACC                                  <  SRR4302540.7430411/94‑1 (MQ=60)

                                                               TCACCGCCTCTCGCGGTCAGGGCGCACGGCATAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGG                                <  SRR4302540.6986782/94‑1 (MQ=60)

                                                               TCACCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGG                                <  SRR4302540.9911059/94‑1 (MQ=60)

                                                                  CCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTT                             <  SRR4302540.9012880/94‑1 (MQ=60)

                                                                  CCGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTT                             <  SRR4302540.7105443/94‑1 (MQ=60)

                                                                   CGCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTC                            <  SRR4302540.9134555/94‑1 (MQ=60)

                                                                    GCCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCC                           >  SRR4302540.2986891/1‑94 (MQ=60)

                                                                     CCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCC                          <  SRR4302540.2722499/94‑1 (MQ=60)

                                                                     CCACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCC                          >  SRR4302540.2653061/1‑94 (MQ=60)

                                                                      CACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCG                         >  SRR4302540.4038634/1‑94 (MQ=60)

                                                                       ACTCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGT                        >  SRR4302540.6451988/1‑94 (MQ=60)

                                                                         TCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTC                      >  SRR4302540.3541642/1‑94 (MQ=60)

                                                                         TCGCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTC                      <  SRR4302540.9273845/94‑1 (MQ=60)

                                                                           GCGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCCGCTTCCCGTTCAA                    >  SRR4302540.8175505/1‑94 (MQ=60)

                                                                            CGGTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGAGACCGGCTTCCCGTTCAAA                   >  SRR4302540.3095382/1‑94 (MQ=60)

                                                                              GTCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTCCTATTCTGGCGACCGGCTTCCCGTTCAAAGC                 >  SRR4302540.1687310/1‑94 (MQ=60)

                                                                               TCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGCTCAAAGCA                >  SRR4302540.10119419/1‑94 (MQ=60)

                                                                               GCAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCA                <  SRR4302540.7855715/94‑1 (MQ=60)

                                                                                CAGGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAA               <  SRR4302540.6008142/94‑1 (MQ=60)

                                                                                  GGGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAA             >  SRR4302540.4192840/1‑94 (MQ=60)

                                                                                   GGCGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAAC            <  SRR4302540.7093717/94‑1 (MQ=60)

                                                                                     CGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAG          <  SRR4302540.7518439/94‑1 (MQ=60)

                                                                                     CGCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAG          <  SRR4302540.1676650/94‑1 (MQ=60)

                                                                                      GCACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGT         <  SRR4302540.8852701/94‑1 (MQ=60)

                                                                                       CACAGCAGAACGGCTACCGGCTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTA        <  SRR4302540.2843041/94‑1 (MQ=60)

                                                                                       CACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTAGTATTGTGGCGACCGGGTTCCCGTTgacagcacaccagtc        >  SRR4302540.7152293/1‑79 (MQ=60)

                                                                                       CACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTA        <  SRR4302540.7316338/94‑1 (MQ=60)

                                                                                       CACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTA        >  SRR4302540.9494014/1‑94 (MQ=60)

                                                                                       CACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTA        <  SRR4302540.4108366/94‑1 (MQ=60)

                                                                                       CACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTA        >  SRR4302540.9225999/1‑94 (MQ=60)

                                                                                       CACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGGTCAAAGCAAAACAGTA        >  SRR4302540.7152291/1‑94 (MQ=60)

                                                                                        ACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTAC       >  SRR4302540.10031469/1‑94 (MQ=60)

                                                                                        ACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTAC       <  SRR4302540.6872585/94‑1 (MQ=60)

                                                                                        ACAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTAC       <  SRR4302540.5776832/94‑1 (MQ=60)

                                                                                         CAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTACG      <  SRR4302540.5951965/94‑1 (MQ=60)

                                                                                         CAGCAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTACG      <  SRR4302540.7467726/94‑1 (MQ=60)

                                                                                            CAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTACGCCA   >  SRR4302540.7837412/1‑94 (MQ=60)

                                                                                            CAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTACGCCA   <  SRR4302540.1830369/94‑1 (MQ=60)

                                                                                            CAGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTACGCCA   <  SRR4302540.7655636/94‑1 (MQ=60)

                                                                                             AGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTACGCCAC  <  SRR4302540.4589187/94‑1 (MQ=60)

                                                                                             AGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTACGCCAC  >  SRR4302540.3208338/1‑94 (MQ=60)

                                                                                             AGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTACGCCAC  >  SRR4302540.7033515/1‑94 (MQ=60)

                                                                                             AGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTACGCCAC  <  SRR4302540.7287851/94‑1 (MQ=60)

CTGTTCGTATCAAAGGCCGCACCGAAGTTGCTGTGGTATACGATCCTATGCGTAACGAACTGTTCACCGCCACTCGCGGTCAGGGCGCACAGCTGAACGGCTACCGACTGCGCGGCAGCACCGCTCGCGATCTCGACGGTACTATTCTGGCGACCGGCTTCCCGTTCAAAGCAAAACAGTACGCCAC  >  NC_000913/2661772‑2661958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`