Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,665,031	A→G	V279A (GTG→GCG)	hcaT ←	HcaT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,665,031	0	A	G	99.6%	702.1 / ‑4.9	224	V279A (GTG→GCG)	hcaT	HcaT
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (91/132); total (92/132)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.63e-01

Only 100 of 240 total aligned reads displayed.

AGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCC  >  NC_000913/2664941‑2665120

 ggTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTa                                                                                        >  2:6667776/1‑93 (MQ=255)

 ggTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTa                                                                                        >  1:5491232/1‑93 (MQ=255)

  gTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTAc                                                                                       >  1:8527491/1‑93 (MQ=255)

  gTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTAc                                                                                       <  2:2121586/93‑1 (MQ=255)

      cAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTAcgccg                                                                                   >  2:8671362/1‑93 (MQ=255)

       aGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTAcgccgc                                                                                  <  2:6220859/93‑1 (MQ=255)

        gACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCa                                                                                 >  1:8622749/1‑93 (MQ=255)

        gACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCa                                                                                 >  2:334289/1‑93 (MQ=255)

         aCCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCaa                                                                                >  2:6255824/1‑93 (MQ=255)

          ccGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCaaa                                                                               <  1:9421737/93‑1 (MQ=255)

          ccGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCaaa                                                                               <  2:3590090/93‑1 (MQ=255)

           cGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAAt                                                                              <  2:2875081/93‑1 (MQ=255)

            gTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATc                                                                             >  2:8410534/1‑93 (MQ=255)

             tGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCg                                                                            >  2:2613080/1‑93 (MQ=255)

                aGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCg                                                                         <  1:6408251/93‑1 (MQ=255)

                 ggTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCTAATCGCCga                                                                        <  2:6738158/93‑1 (MQ=255)

                 ggTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCga                                                                        <  2:1263378/93‑1 (MQ=255)

                  gTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCgag                                                                       >  2:7589869/1‑93 (MQ=255)

                   tGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCgaga                                                                      <  1:4896040/93‑1 (MQ=255)

                       gCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCaa                                                                  <  1:1008735/93‑1 (MQ=255)

                       gCAAGGCAGAATTTCCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCaa                                                                  <  1:8217271/93‑1 (MQ=255)

                        cAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAAc                                                                 <  2:10043360/93‑1 (MQ=255)

                         aaTGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGCTCAACa                                                                >  1:8109176/1‑93 (MQ=255)

                           tGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGc                                                              >  1:6858669/1‑93 (MQ=255)

                            gCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCa                                                             >  1:9909877/1‑93 (MQ=255)

                            gCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCa                                                             <  2:7360685/93‑1 (MQ=255)

                              aGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCata                                                           <  1:3026446/93‑1 (MQ=255)

                              aGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCata                                                           <  2:9219267/93‑1 (MQ=255)

                              aGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATG‑CCCAGCGCGCTACGCCGCCAATCGCCGAGATCAACAGCatat                                                          >  1:3074590/1‑93 (MQ=255)

                               gAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCatat                                                          <  1:923634/93‑1 (MQ=255)

                               gAATTTGCACCACTATCAACCACGACAACGCCATAGTTACACCCATAATGCCCCAGCGCGCAACGCAGCAAATCACCGAGATCAACCGCaaat                                                          <  2:7718539/93‑1 (MQ=255)

                               gAATTGGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCatat                                                          <  1:4857127/93‑1 (MQ=255)

                                aaTTTTCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATc                                                         <  2:4990413/93‑1 (MQ=255)

                                aaTTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATc                                                         <  2:187689/93‑1 (MQ=255)

                                 aTTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATcg                                                        <  1:2628879/93‑1 (MQ=255)

                                  tttGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATcgc                                                       >  2:9834506/1‑93 (MQ=255)

                                  tttGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATcgc                                                       >  1:4814772/1‑93 (MQ=255)

                                       cccacTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCa                                                  <  2:2230483/92‑1 (MQ=255)

                                       cccacTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCa                                                  <  1:4195026/92‑1 (MQ=255)

                                       accacTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCa                                                  >  1:7184408/1‑93 (MQ=255)

                                        ccacTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCAc                                                 >  2:1799772/1‑93 (MQ=255)

                                        ccacTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCAc                                                 <  1:2878247/93‑1 (MQ=255)

                                           cTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTAc                                              >  2:9673967/1‑93 (MQ=255)

                                            tATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACa                                             >  1:5038681/1‑93 (MQ=255)

                                             aTCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACaa                                            >  1:27359/1‑93 (MQ=255)

                                             aTCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACaa                                            <  2:6458034/93‑1 (MQ=255)

                                               cAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAAcg                                          <  2:8145192/93‑1 (MQ=255)

                                                 aCCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAAcggc                                        >  1:2085766/1‑93 (MQ=255)

                                                  ccACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAAcggcg                                       >  2:8012453/1‑93 (MQ=255)

                                                   cACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAAcggcgg                                      >  2:6787008/1‑93 (MQ=255)

                                                    aCGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGa                                     <  1:4316833/93‑1 (MQ=255)

                                                      ggCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGaaa                                   >  1:9648813/1‑93 (MQ=255)

                                                       gCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGaaaa                                  >  1:1197179/1‑93 (MQ=255)

                                                         aaCGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGt                                <  1:446837/93‑1 (MQ=255)

                                                         aaCGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGt                                >  2:9597/1‑93 (MQ=255)

                                                          aCGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGtt                               <  2:5720565/93‑1 (MQ=255)

                                                          aCGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGtt                               >  2:7270641/1‑93 (MQ=255)

                                                           cGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGttt                              <  1:7858304/93‑1 (MQ=255)

                                                           cGCCGTAGTGCCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGttt                              <  2:3041217/93‑1 (MQ=255)

                                                             ccGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTttat                            <  1:1734022/93‑1 (MQ=255)

                                                               gTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTttattc                          >  2:5274523/1‑92 (MQ=255)

                                                               gTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTttatta                          >  1:6550829/1‑93 (MQ=255)

                                                               gTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTttatta                          <  1:2662558/93‑1 (MQ=255)

                                                               gTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTttatta                          >  1:9148140/1‑93 (MQ=255)

                                                                tAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTAc                         <  2:4325327/93‑1 (MQ=255)

                                                                tAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTAc                         >  2:911546/1‑93 (MQ=255)

                                                                 aGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACt                        <  2:4429418/93‑1 (MQ=255)

                                                                 aGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACt                        >  1:3378493/1‑93 (MQ=255)

                                                                  gTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTc                       >  1:4576507/1‑93 (MQ=255)

                                                                   ttGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTATTcc                      >  1:3142838/1‑92 (MQ=255)

                                                                    tGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAg                     <  1:5284442/93‑1 (MQ=255)

                                                                     gCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAgc                    <  1:6075327/93‑1 (MQ=255)

                                                                       taccATAATGCCTCAGCGCGCTGCGCCGCAGATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAgcgc                  <  1:9962101/91‑1 (MQ=255)

                                                                        cccATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCa                 >  1:8728325/1‑93 (MQ=255)

                                                                         ccATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCaa                >  2:7887815/1‑93 (MQ=255)

                                                                         ccATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCaa                <  2:5010531/93‑1 (MQ=255)

                                                                           aTAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAg              <  2:1660977/93‑1 (MQ=255)

                                                                           aTAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAg              <  2:6622807/93‑1 (MQ=255)

                                                                            tAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGa             >  2:5879902/1‑93 (MQ=255)

                                                                            tAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAAAGGCGGCAAAGTTTATTACTCAGCGCAAAGa             >  2:5413545/1‑93 (MQ=255)

                                                                              aTGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATa           <  1:4417456/93‑1 (MQ=255)

                                                                              aTGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATa           <  2:852234/93‑1 (MQ=255)

                                                                              aTGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATa           <  2:3814370/93‑1 (MQ=255)

                                                                               tGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATaa          <  1:2571841/93‑1 (MQ=255)

                                                                               tGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATaa          <  1:2418394/93‑1 (MQ=255)

                                                                               tGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATaa          <  1:5591586/93‑1 (MQ=255)

                                                                               tGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATaa          <  2:8805112/93‑1 (MQ=255)

                                                                                 ccccAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATg        <  1:2917113/93‑1 (MQ=255)

                                                                                   ccAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGAc      >  2:7603938/1‑93 (MQ=255)

                                                                                   ccAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGAc      <  2:1773908/93‑1 (MQ=255)

                                                                                   ccAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGAc      <  2:5455239/93‑1 (MQ=255)

                                                                                    cAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACt     <  1:7614661/93‑1 (MQ=255)

                                                                                    cAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACt     >  1:1480072/1‑93 (MQ=255)

                                                                                     aGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACtt    <  2:4541482/93‑1 (MQ=255)

                                                                                     aGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACtt    >  2:8237751/1‑93 (MQ=255)

                                                                                     aGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACtt    <  1:1296552/93‑1 (MQ=255)

                                                                                     aGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACtt    <  2:4862039/93‑1 (MQ=255)

                                                                                      gcgcgcTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTc   >  1:3218001/1‑93 (MQ=255)

                                                                                      gcgcgcTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTc   <  2:2322052/93‑1 (MQ=255)

                                                                                       cgcgCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTcc  <  1:742484/93‑1 (MQ=255)

AGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCC  >  NC_000913/2664941‑2665120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCA  >  NC_000913/2664938‑2665124

GCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCG                                                                                               >  SRR4302540.4990756/1‑94 (MQ=60)

 CCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGC                                                                                              >  SRR4302540.6115172/1‑94 (MQ=60)

  CAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCT                                                                                             <  SRR4302540.1480301/94‑1 (MQ=60)

   AGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTA                                                                                            >  SRR4302540.5551688/1‑94 (MQ=60)

    GGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTAC                                                                                           >  SRR4302540.8626574/1‑94 (MQ=60)

     GTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACG                                                                                          <  SRR4302540.4428565/94‑1 (MQ=60)

       GGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCC                                                                                        <  SRR4302540.1532801/94‑1 (MQ=60)

          AGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCA                                                                                     >  SRR4302540.8722915/1‑94 (MQ=60)

             CCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAAT                                                                                  <  SRR4302540.9533715/94‑1 (MQ=60)

             CCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAAT                                                                                  <  SRR4302540.7545954/94‑1 (MQ=60)

              CGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATC                                                                                 <  SRR4302540.4889885/94‑1 (MQ=60)

              CGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATC                                                                                 >  SRR4302540.3000430/1‑94 (MQ=60)

              CGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATC                                                                                 >  SRR4302540.5462893/1‑94 (MQ=60)

              CGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATC                                                                                 <  SRR4302540.815003/94‑1 (MQ=60)

               GTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCC                                                                                >  SRR4302540.4657274/1‑94 (MQ=60)

                  AAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCG                                                                             <  SRR4302540.7847037/94‑1 (MQ=60)

                   AGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGA                                                                            <  SRR4302540.6479572/94‑1 (MQ=60)

                    GGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAG                                                                           <  SRR4302540.4418449/94‑1 (MQ=60)

                     GTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGA                                                                          >  SRR4302540.5642200/1‑94 (MQ=60)

                      TGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGAT                                                                         <  SRR4302540.4948701/94‑1 (MQ=60)

                       GCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATC                                                                        <  SRR4302540.7481682/94‑1 (MQ=60)

                        GCGCAATGCAGAATTTGCACCTCTATCAGCCACGGCAGCGCCGTAGTTGCTCCCATAAGGCCCCAGCGCGCTACGCCGCAAATCTCCGAGATCA                                                                       <  SRR4302540.7154267/94‑1 (MQ=60)

                        CCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCA                                                                       >  SRR4302540.4555257/1‑94 (MQ=60)

                        CCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCA                                                                       <  SRR4302540.8886472/94‑1 (MQ=60)

                        CCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCA                                                                       >  SRR4302540.3025730/1‑94 (MQ=60)

                         CGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAA                                                                      <  SRR4302540.2700458/94‑1 (MQ=60)

                         CGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAA                                                                      >  SRR4302540.9297773/1‑94 (MQ=60)

                          GCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAAC                                                                     <  SRR4302540.8312781/94‑1 (MQ=60)

                          GCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAAC                                                                     <  SRR4302540.1018966/94‑1 (MQ=60)

                          GCAAGGCAGAATTTCCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAAC                                                                     <  SRR4302540.8312780/94‑1 (MQ=60)

                           CAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGCTCAACA                                                                    >  SRR4302540.8203296/1‑94 (MQ=60)

                           CAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACA                                                                    <  SRR4302540.8228012/94‑1 (MQ=60)

                            AATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAG                                                                   <  SRR4302540.7790251/94‑1 (MQ=60)

                             ATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGC                                                                  >  SRR4302540.6934673/1‑94 (MQ=60)

                             ATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGC                                                                  >  SRR4302540.5451100/1‑94 (MQ=60)

                              TGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCA                                                                 >  SRR4302540.10025627/1‑94 (MQ=60)

                                CAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATG‑CCCAGCGCGCTACGCCGCCAATCGCCGAGATCAACAGCATAT                                                              >  SRR4302540.3108316/1‑94 (MQ=60)

                                 AGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATAT                                                              <  SRR4302540.1189152/94‑1 (MQ=60)

                                 AGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATAT                                                              <  SRR4302540.3059687/94‑1 (MQ=60)

                                  GAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATC                                                             <  SRR4302540.4909201/94‑1 (MQ=60)

                                  GAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATC                                                             <  SRR4302540.933373/94‑1 (MQ=60)

                                  GAATTGGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATC                                                             <  SRR4302540.4909200/94‑1 (MQ=60)

                                   AATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCG                                                            <  SRR4302540.812233/94‑1 (MQ=60)

                                    ATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGC                                                           >  SRR4302540.4866304/1‑94 (MQ=60)

                                    ATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGC                                                           >  SRR4302540.10078006/1‑94 (MQ=60)

                                    ATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGC                                                           <  SRR4302540.2657328/94‑1 (MQ=60)

                                    ATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGC                                                           >  SRR4302540.2360962/1‑94 (MQ=60)

                                         CACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCA                                                      >  SRR4302540.7264500/1‑94 (MQ=60)

                                          CCCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCAC                                                     <  SRR4302540.4240659/94‑1 (MQ=60)

                                           CCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACT                                                    <  SRR4302540.2909548/94‑1 (MQ=60)

                                             CCTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTAC                                                  <  SRR4302540.3258698/94‑1 (MQ=60)

                                             ACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTAC                                                  >  SRR4302540.10097616/1‑94 (MQ=60)

                                              CTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGGTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGgacacca                                                 >  SRR4302540.3604089/1‑87 (MQ=60)

                                              CTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACA                                                 >  SRR4302540.6351175/1‑94 (MQ=60)

                                              CTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACA                                                 >  SRR4302540.5093188/1‑94 (MQ=60)

                                              CTATCAACCACGGCAACGCCGTAGTTGCCCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACA                                                 >  SRR4302540.937167/1‑94 (MQ=60)

                                               TATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAA                                                >  SRR4302540.27652/1‑94 (MQ=60)

                                                ATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAAC                                               >  SRR4302540.8332433/1‑94 (MQ=60)

                                                ATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAAC                                               <  SRR4302540.261581/94‑1 (MQ=60)

                                                ATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAAC                                               >  SRR4302540.8888193/1‑94 (MQ=60)

                                                  CAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGG                                             <  SRR4302540.7293992/94‑1 (MQ=60)

                                                   AACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGC                                            >  SRR4302540.2108174/1‑94 (MQ=60)

                                                       ACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAA                                        <  SRR4302540.4363831/94‑1 (MQ=60)

                                                        CGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAA                                       >  SRR4302540.9762436/1‑94 (MQ=60)

                                                         GGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAA                                      >  SRR4302540.1209496/1‑94 (MQ=60)

                                                          GCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAG                                     <  SRR4302540.2945255/94‑1 (MQ=60)

                                                          GCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGCCGGAAAAG                                     >  SRR4302540.2672246/1‑94 (MQ=60)

                                                           gcacggcggtagttccCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGT                                    <  SRR4302540.3762130/78‑1 (MQ=60)

                                                            AACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTT                                   <  SRR4302540.5194043/94‑1 (MQ=60)

                                                            AACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTT                                   <  SRR4302540.451600/94‑1 (MQ=60)

                                                            AACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTT                                   >  SRR4302540.6592823/1‑94 (MQ=60)

                                                             ACGCCGTATTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTT                                  <  SRR4302540.9449439/94‑1 (MQ=60)

                                                             ACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTT                                  <  SRR4302540.324109/94‑1 (MQ=60)

                                                              CGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTA                                 <  SRR4302540.7950122/94‑1 (MQ=60)

                                                              CGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTA                                 <  SRR4302540.6825777/94‑1 (MQ=60)

                                                                CCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATT                               <  SRR4302540.1752738/94‑1 (MQ=60)

                                                                CCGTAGTTGCTCCCATAATGCCCCAGCGCGCGACGCCGCAAAGCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATT                               <  SRR4302540.8697087/94‑1 (MQ=60)

                                                                 CGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTA                              >  SRR4302540.9258001/1‑94 (MQ=60)

                                                                 CGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTA                              >  SRR4302540.6623489/1‑94 (MQ=60)

                                                                  GTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTGTTAC                             >  SRR4302540.5747956/1‑94 (MQ=60)

                                                                  GTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTAC                             <  SRR4302540.2691439/94‑1 (MQ=60)

                                                                  GTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTAC                             >  SRR4302540.206452/1‑94 (MQ=60)

                                                                  GTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACAGCGGAAAAGATTATTAC                             >  SRR4302540.4477667/1‑94 (MQ=60)

                                                                   TAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACT                            >  SRR4302540.3415388/1‑94 (MQ=60)

                                                                   TAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACT                            <  SRR4302540.3171316/94‑1 (MQ=60)

                                                                   TAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACT                            <  SRR4302540.4072554/94‑1 (MQ=60)

                                                                    AGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTC                           >  SRR4302540.4625870/1‑94 (MQ=60)

                                                                     GTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTAttcc                          >  SRR4302540.3177099/1‑90 (MQ=60)

                                                                     GTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCA                          <  SRR4302540.2725456/94‑1 (MQ=60)

                                                                     GTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCA                          <  SRR4302540.1439310/94‑1 (MQ=60)

                                                                     GTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCA                          <  SRR4302540.698079/94‑1 (MQ=60)

                                                                      TTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAG                         <  SRR4302540.2549069/94‑1 (MQ=60)

                                                                       TGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGC                        <  SRR4302540.5342185/94‑1 (MQ=60)

                                                                        GCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCG                       <  SRR4302540.6142881/94‑1 (MQ=60)

                                                                          TCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAACGTTTATTACTCAGCGCA                     >  SRR4302540.8806547/1‑94 (MQ=60)

                                                                          TCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCA                     >  SRR4302540.8829682/1‑94 (MQ=60)

                                                                          TCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCA                     <  SRR4302540.10078339/94‑1 (MQ=60)

                                                                          TACCATAATGCCTCAGCGCGCTGCGCCGCAGATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCA                     <  SRR4302540.10078340/94‑1 (MQ=60)

                                                                           CCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAA                    >  SRR4302540.1439430/1‑94 (MQ=60)

                                                                             CATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAG                  <  SRR4302540.9850924/94‑1 (MQ=60)

                                                                              ATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGA                 >  SRR4302540.4795379/1‑94 (MQ=60)

                                                                              ATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGA                 >  SRR4302540.2655186/1‑94 (MQ=60)

                                                                              ATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGA                 >  SRR4302540.5912911/1‑94 (MQ=60)

                                                                              ATAATGCCCCAGCGCCCTAAGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGA                 <  SRR4302540.9566110/94‑1 (MQ=60)

                                                                                AATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATA               <  SRR4302540.4917325/94‑1 (MQ=60)

                                                                                 ATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAA              <  SRR4302540.4465320/94‑1 (MQ=60)

                                                                                 ATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAA              >  SRR4302540.310225/1‑94 (MQ=60)

                                                                                 ATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAA              <  SRR4302540.6648644/94‑1 (MQ=60)

                                                                                 ATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAA              >  SRR4302540.4994353/1‑94 (MQ=60)

                                                                                  TGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAAT             <  SRR4302540.2444460/94‑1 (MQ=60)

                                                                                  TGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAAT             <  SRR4302540.5653109/94‑1 (MQ=60)

                                                                                  TGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAAT             <  SRR4302540.2599566/94‑1 (MQ=60)

                                                                                    CCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGA           <  SRR4302540.2949038/94‑1 (MQ=60)

                                                                                      CCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACT         <  SRR4302540.2826866/94‑1 (MQ=60)

                                                                                      CCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACT         >  SRR4302540.1496284/1‑94 (MQ=60)

                                                                                       CAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTT        <  SRR4302540.7700263/94‑1 (MQ=60)

                                                                                        AGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTC       <  SRR4302540.1309975/94‑1 (MQ=60)

                                                                                        AGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTC       <  SRR4302540.1309976/94‑1 (MQ=60)

                                                                                        AGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTC       >  SRR4302540.3252953/1‑94 (MQ=60)

                                                                                         GCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCC      <  SRR4302540.4297323/94‑1 (MQ=60)

                                                                                         GCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCC      <  SRR4302540.3149367/94‑1 (MQ=60)

                                                                                         GCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCC      <  SRR4302540.1966758/94‑1 (MQ=60)

                                                                                          CGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG     <  SRR4302540.750405/94‑1 (MQ=60)

                                                                                             GCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGATTATTACTCAGCGCAAAGATAATGACTTCCGCCA  >  SRR4302540.5406290/1‑94 (MQ=60)

GCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCA  >  NC_000913/2664938‑2665124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`