Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,665,031	A→G	V279A (GTG→GCG)	hcaT ←	HcaT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,665,031	0	A	G	100.0%	695.5 / NA	218	V279A (GTG→GCG)	hcaT	HcaT
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (98/120); total (98/120)

Only 100 of 229 total aligned reads displayed.

GGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCC  >  NC_000913/2664942‑2665120

ggTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTa                                                                                        >  1:820033/1‑93 (MQ=255)

 gTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTAc                                                                                       >  2:5101222/1‑93 (MQ=255)

 gTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTAc                                                                                       <  2:5335123/93‑1 (MQ=255)

  tGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTAcg                                                                                      >  1:153742/1‑93 (MQ=255)

   ggCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTAcgc                                                                                     <  2:224820/93‑1 (MQ=255)

    gCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTAcgcc                                                                                    <  1:6023748/93‑1 (MQ=255)

     cAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTAcgccg                                                                                   <  2:1991759/93‑1 (MQ=255)

     cAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTAcgccg                                                                                   >  2:2726375/1‑93 (MQ=255)

      aGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTAcgccgc                                                                                  >  2:187389/1‑93 (MQ=255)

      aGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTAcgccgc                                                                                  >  2:5648244/1‑93 (MQ=255)

       gACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCa                                                                                 >  2:5385047/1‑93 (MQ=255)

       gACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCa                                                                                 >  2:359245/1‑93 (MQ=255)

       gACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCa                                                                                 >  2:4881915/1‑93 (MQ=255)

       gACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCa                                                                                 <  1:218492/93‑1 (MQ=255)

       gACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCa                                                                                 >  1:1420662/1‑93 (MQ=255)

        aCCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCaa                                                                                <  1:109440/93‑1 (MQ=255)

         ccGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCaaa                                                                               <  1:5741027/93‑1 (MQ=255)

          cGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAGCGCCGTAGTTGCTCCCATAATGCCCCAGCGCGTTACGCCGCAAAt                                                                              <  1:2257095/93‑1 (MQ=255)

          cGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAAt                                                                              <  1:322991/93‑1 (MQ=255)

           gTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATc                                                                             <  2:1188165/93‑1 (MQ=255)

           gTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATc                                                                             >  1:1786580/1‑93 (MQ=255)

           gTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATc                                                                             <  1:6303923/93‑1 (MQ=255)

            tGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCg                                                                            >  2:2183231/1‑93 (MQ=255)

             gAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGc                                                                           <  1:1292313/93‑1 (MQ=255)

             gAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGc                                                                           >  2:3452514/1‑93 (MQ=255)

               aGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCg                                                                         <  1:2523270/93‑1 (MQ=255)

                   gCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGAt                                                                     <  1:5366276/93‑1 (MQ=255)

                     cGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCa                                                                   >  1:2398173/1‑93 (MQ=255)

                      gCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCaa                                                                  >  2:2951384/1‑93 (MQ=255)

                       cAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAAc                                                                 >  2:4964430/1‑93 (MQ=255)

                       cAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAAc                                                                 >  2:3803773/1‑93 (MQ=255)

                       cAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAAc                                                                 >  1:6230294/1‑93 (MQ=255)

                          tGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGc                                                              >  1:1685282/1‑93 (MQ=255)

                          tGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGc                                                              >  2:3985242/1‑93 (MQ=255)

                          tGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGc                                                              >  2:3206875/1‑93 (MQ=255)

                           gCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCa                                                             >  1:3793058/1‑93 (MQ=255)

                           gCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCa                                                             >  2:193847/1‑93 (MQ=255)

                              gAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCatat                                                          <  1:3342058/93‑1 (MQ=255)

                              gAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCatat                                                          <  2:5195333/93‑1 (MQ=255)

                               aaTTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATc                                                         <  2:1107930/93‑1 (MQ=255)

                                 tttGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATcgc                                                       >  2:135577/1‑93 (MQ=255)

                                 tttGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATcgc                                                       >  2:5930348/1‑93 (MQ=255)

                                 tttGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATcgc                                                       >  1:879280/1‑93 (MQ=255)

                                      accacTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCa                                                  >  2:3746958/1‑93 (MQ=255)

                                        cacTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACt                                                >  1:4134452/1‑93 (MQ=255)

                                         acTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTa                                               <  2:2763123/93‑1 (MQ=255)

                                          cTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTAc                                              <  2:3679848/93‑1 (MQ=255)

                                          cTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTAc                                              >  2:6241105/1‑93 (MQ=255)

                                            aTCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACaa                                            <  2:1588486/93‑1 (MQ=255)

                                            aTCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACaa                                            >  1:4995304/1‑93 (MQ=255)

                                            aTCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACaa                                            >  1:5495966/1‑93 (MQ=255)

                                            aTCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACaa                                            <  1:108655/93‑1 (MQ=255)

                                            aTCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACaa                                            <  1:4075692/93‑1 (MQ=255)

                                            aTCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACaa                                            >  2:428226/1‑93 (MQ=255)

                                              cAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACTCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAAcg                                          <  2:2894145/93‑1 (MQ=255)

                                               aaCCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAAcgg                                         <  2:2443970/93‑1 (MQ=255)

                                                aCCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAAcggc                                        <  1:20144/93‑1 (MQ=255)

                                                aCCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAAaggc                                        >  2:3351962/1‑93 (MQ=255)

                                                  cACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAAcggcgg                                      <  1:4531533/93‑1 (MQ=255)

                                                    cGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGaa                                    >  1:5429332/1‑93 (MQ=255)

                                                    cGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGaa                                    <  2:2223860/93‑1 (MQ=255)

                                                    cGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGaa                                    <  2:5958108/93‑1 (MQ=255)

                                                     ggCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGaaa                                   <  1:394710/93‑1 (MQ=255)

                                                     ggCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGaaa                                   <  2:5294192/93‑1 (MQ=255)

                                                         aCGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGtt                               <  1:5158140/93‑1 (MQ=255)

                                                         aCGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGtt                               >  2:4805797/1‑93 (MQ=255)

                                                          cGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGttt                              >  2:4332508/1‑93 (MQ=255)

                                                          cGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGttt                              <  2:844035/93‑1 (MQ=255)

                                                          cGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGttt                              <  1:5723260/93‑1 (MQ=255)

                                                           ggcGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGATATCAACAGCATATCGCGTGAACTACAACGGCGGAAAAGTtta                             <  2:3060417/91‑1 (MQ=255)

                                                           gCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTtta                             >  2:1017783/1‑93 (MQ=255)

                                                           gCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTtta                             >  2:2590336/1‑93 (MQ=255)

                                                            ccGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTttat                            <  2:2115847/93‑1 (MQ=255)

                                                            ccGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTttat                            <  1:4709160/93‑1 (MQ=255)

                                                            ccGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTttat                            >  1:3192293/1‑93 (MQ=255)

                                                              gTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTttatta                          <  1:2277034/93‑1 (MQ=255)

                                                              gTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTttatta                          >  2:4225703/1‑93 (MQ=255)

                                                               tAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAGATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTAc                         <  1:405527/93‑1 (MQ=255)

                                                               tAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTAc                         <  1:1001468/93‑1 (MQ=255)

                                                                aGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACt                        >  1:2888175/1‑93 (MQ=255)

                                                                aGTTGCTCCCATAATCCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACt                        <  2:877512/93‑1 (MQ=255)

                                                                  ttGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCa                      <  1:2663197/93‑1 (MQ=255)

                                                                    gCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAgc                    <  2:2333326/93‑1 (MQ=255)

                                                                    gCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAgc                    >  2:547757/1‑93 (MQ=255)

                                                                      tCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAgcgc                  >  1:2979427/1‑93 (MQ=255)

                                                                       cccATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCa                 >  1:2458310/1‑93 (MQ=255)

                                                                       cccATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCa                 >  1:1191043/1‑93 (MQ=255)

                                                                       cccATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCa                 >  2:2270092/1‑93 (MQ=255)

                                                                          aTAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAg              <  2:5429332/93‑1 (MQ=255)

                                                                          aTAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAg              <  1:5552602/93‑1 (MQ=255)

                                                                            aaTGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGAt            <  1:5807273/93‑1 (MQ=255)

                                                                             aTGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATa           >  1:3986547/1‑93 (MQ=255)

                                                                               gCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAAt         <  2:5274832/93‑1 (MQ=255)

                                                                                ccccAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATg        <  2:1260116/93‑1 (MQ=255)

                                                                                  ccAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGAc      >  1:2804479/1‑93 (MQ=255)

                                                                                   cAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACt     >  1:4416698/1‑93 (MQ=255)

                                                                                   cAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACt     >  1:5518024/1‑93 (MQ=255)

                                                                                     gcgcgcTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTc   >  1:3823108/1‑93 (MQ=255)

                                                                                     gcgcgcTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTc   <  1:367197/93‑1 (MQ=255)

                                                                                      cgcgCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTcc  <  1:1351739/93‑1 (MQ=255)

GGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCC  >  NC_000913/2664942‑2665120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCA  >  NC_000913/2664941‑2665124

AGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTA                                                                                            >  SRR4302533.2713205/1‑94 (MQ=60)

AGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTA                                                                                            >  SRR4302533.824841/1‑94 (MQ=60)

 GGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTAC                                                                                           <  SRR4302533.6161312/94‑1 (MQ=60)

  GTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACG                                                                                          >  SRR4302533.154723/1‑94 (MQ=60)

    GGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCC                                                                                        <  SRR4302533.38531/94‑1 (MQ=60)

    GGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCC                                                                                        <  SRR4302533.5548655/94‑1 (MQ=60)

    GGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCC                                                                                        >  SRR4302533.5975607/1‑94 (MQ=60)

     GCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCG                                                                                       <  SRR4302533.904030/94‑1 (MQ=60)

     GCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCG                                                                                       <  SRR4302533.570044/94‑1 (MQ=60)

     GCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCG                                                                                       <  SRR4302533.6060252/94‑1 (MQ=60)

      CAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGC                                                                                      <  SRR4302533.1229009/94‑1 (MQ=60)

      CAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGC                                                                                      >  SRR4302533.5863079/1‑94 (MQ=60)

       AGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCA                                                                                     >  SRR4302533.1429257/1‑94 (MQ=60)

        GACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAA                                                                                    <  SRR4302533.219860/94‑1 (MQ=60)

         ACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAA                                                                                   <  SRR4302533.110136/94‑1 (MQ=60)

          CCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAAT                                                                                  <  SRR4302533.4979150/94‑1 (MQ=60)

          CCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAAT                                                                                  <  SRR4302533.2520601/94‑1 (MQ=60)

          CCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAAT                                                                                  <  SRR4302533.5775670/94‑1 (MQ=60)

           CGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAGCGCCGTAGTTGCTCCCATAATGCCCCAGCGCGTTACGCCGCAAATC                                                                                 <  SRR4302533.2271061/94‑1 (MQ=60)

           CGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATC                                                                                 <  SRR4302533.324962/94‑1 (MQ=60)

           CGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATC                                                                                 >  SRR4302533.1797441/1‑94 (MQ=60)

            GTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCG                                                                                <  SRR4302533.6341615/94‑1 (MQ=60)

            GTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCG                                                                                <  SRR4302533.1170016/94‑1 (MQ=60)

              GAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCC                                                                              <  SRR4302533.1300096/94‑1 (MQ=60)

                AGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGA                                                                            <  SRR4302533.2538815/94‑1 (MQ=60)

                 GGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAG                                                                           <  SRR4302533.413604/94‑1 (MQ=60)

                  GTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGA                                                                          <  SRR4302533.1204236/94‑1 (MQ=60)

                  GTGCCGCAATGCAGAATTCGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCCCTACGCCGCAAATCGGCGAGA                                                                          >  SRR4302533.241997/1‑94 (MQ=60)

                    GCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATC                                                                        <  SRR4302533.5398510/94‑1 (MQ=60)

                     CCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCA                                                                       >  SRR4302533.2412976/1‑94 (MQ=60)

                      CGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAA                                                                      >  SRR4302533.2040986/1‑94 (MQ=60)

                       GCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAAC                                                                     >  SRR4302533.6267607/1‑94 (MQ=60)

                        CAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACA                                                                    >  SRR4302533.5760009/1‑94 (MQ=60)

                        CAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACA                                                                    <  SRR4302533.5786764/94‑1 (MQ=60)

                         AATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAG                                                                   >  SRR4302533.878879/1‑94 (MQ=60)

                         AATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAG                                                                   <  SRR4302533.834776/94‑1 (MQ=60)

                          ATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGC                                                                  >  SRR4302533.3799427/1‑94 (MQ=60)

                          ATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGC                                                                  >  SRR4302533.1695491/1‑94 (MQ=60)

                           TGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCA                                                                 >  SRR4302533.3816274/1‑94 (MQ=60)

                            GCAGAATTTGCACCACTATCAACCATGGCAACGCCGGAGCTGCGCCTATTATGCCTCAGCGCGCTACGCCGCAATTCGCCGAGATCAACAGCAT                                                                >  SRR4302533.6335846/1‑94 (MQ=53)

                             CAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATA                                                               >  SRR4302533.4088017/1‑94 (MQ=60)

                              AGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATAT                                                              <  SRR4302533.377898/94‑1 (MQ=60)

                               GAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATC                                                             <  SRR4302533.3362715/94‑1 (MQ=60)

                               GAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATC                                                             <  SRR4302533.3118356/94‑1 (MQ=60)

                                AATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCG                                                            <  SRR4302533.2736125/94‑1 (MQ=60)

                                AATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCG                                                            <  SRR4302533.4072987/94‑1 (MQ=60)

                                AATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCG                                                            >  SRR4302533.4532144/1‑94 (MQ=60)

                                AATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCG                                                            <  SRR4302533.5579292/94‑1 (MQ=60)

                                 ATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGC                                                           >  SRR4302533.884441/1‑94 (MQ=60)

                                      CACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCA                                                      <  SRR4302533.2976276/94‑1 (MQ=60)

                                        CCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACT                                                    >  SRR4302533.4159547/1‑94 (MQ=60)

                                         CACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGGGATTAACACCATATCGCAGGCACTA                                                   >  SRR4302533.4192679/1‑94 (MQ=60)

                                          ACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCGCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTAA                                                  >  SRR4302533.1465699/1‑94 (MQ=60)

                                           CTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACA                                                 >  SRR4302533.172841/1‑94 (MQ=60)

                                            TATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAA                                                >  SRR4302533.5529114/1‑94 (MQ=60)

                                            TATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAA                                                <  SRR4302533.5169753/94‑1 (MQ=60)

                                            TATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAA                                                >  SRR4302533.5025587/1‑94 (MQ=60)

                                             ATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAAC                                               <  SRR4302533.109340/94‑1 (MQ=60)

                                             ATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAAC                                               <  SRR4302533.4100503/94‑1 (MQ=60)

                                              TCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACG                                              <  SRR4302533.4736647/94‑1 (MQ=60)

                                                AACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGC                                            >  SRR4302533.1097040/1‑94 (MQ=60)

                                                AACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGC                                            <  SRR4302533.5593108/94‑1 (MQ=60)

                                                 ACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCG                                           <  SRR4302533.20281/94‑1 (MQ=60)

                                                  CCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGG                                          <  SRR4302533.3822431/94‑1 (MQ=60)

                                                   CACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGA                                         >  SRR4302533.5412952/1‑94 (MQ=60)

                                                   CACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGA                                         <  SRR4302533.4558972/94‑1 (MQ=60)

                                                    ACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAA                                        <  SRR4302533.2058907/94‑1 (MQ=60)

                                                    ACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAA                                        >  SRR4302533.5462032/1‑94 (MQ=60)

                                                      GGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAA                                      <  SRR4302533.397104/94‑1 (MQ=60)

                                                       GCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAG                                     <  SRR4302533.3503180/94‑1 (MQ=60)

                                                          ACGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTT                                  <  SRR4302533.5189278/94‑1 (MQ=60)

                                                           CGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTA                                 <  SRR4302533.4934111/94‑1 (MQ=60)

                                                           CGCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTA                                 <  SRR4302533.5757813/94‑1 (MQ=60)

                                                            GCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTAT                                >  SRR4302533.3983111/1‑94 (MQ=60)

                                                            GCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTAT                                <  SRR4302533.5446613/94‑1 (MQ=60)

                                                            GCCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTAT                                >  SRR4302533.3211931/1‑94 (MQ=60)

                                                             CCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATT                               <  SRR4302533.2824913/94‑1 (MQ=60)

                                                             CCGTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATT                               <  SRR4302533.4737702/94‑1 (MQ=60)

                                                               GTAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTAC                             <  SRR4302533.2291135/94‑1 (MQ=60)

                                                                TAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAGATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACT                            <  SRR4302533.407964/94‑1 (MQ=60)

                                                                TAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACT                            >  SRR4302533.2905929/1‑94 (MQ=60)

                                                                TAGTTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACT                            <  SRR4302533.1007315/94‑1 (MQ=60)

                                                                   TTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAG                         <  SRR4302533.2607608/94‑1 (MQ=60)

                                                                   TTGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAG                         <  SRR4302533.2679547/94‑1 (MQ=60)

                                                                    TGCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGC                        <  SRR4302533.4386124/94‑1 (MQ=60)

                                                                     GCTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCG                       <  SRR4302533.2264723/94‑1 (MQ=60)

                                                                      CTCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGC                      >  SRR4302533.2997658/1‑94 (MQ=60)

                                                                       TCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCA                     >  SRR4302533.2473478/1‑94 (MQ=60)

                                                                       TCCCATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCA                     >  SRR4302533.1198199/1‑94 (MQ=60)

                                                                          CATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAG                  <  SRR4302533.185285/94‑1 (MQ=60)

                                                                           ATAATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGA                 <  SRR4302533.5586134/94‑1 (MQ=60)

                                                                             AATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATA               >  SRR4302533.4010828/1‑94 (MQ=60)

                                                                             AATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATA               <  SRR4302533.5842339/94‑1 (MQ=60)

                                                                             AATGCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATA               <  SRR4302533.528155/94‑1 (MQ=60)

                                                                                GCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTNTTACTCAGCGCAAAGATAATG            >  SRR4302533.2325154/1‑94 (MQ=60)

                                                                                GCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATG            <  SRR4302533.1537949/94‑1 (MQ=60)

                                                                                GCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATG            <  SRR4302533.1073383/94‑1 (MQ=60)

                                                                                GCCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATG            <  SRR4302533.661090/94‑1 (MQ=60)

                                                                                 CCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGTAAAAGTTTATTACTCAGCGCAAAGATAATGA           <  SRR4302533.2287285/94‑1 (MQ=60)

                                                                                 CCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGA           <  SRR4302533.1309027/94‑1 (MQ=60)

                                                                                 CCCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGAGGAAAAGTTTATTACTCAGCGCAAAGATAATGC           >  SRR4302533.1622600/1‑94 (MQ=60)

                                                                                  CCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGAC          <  SRR4302533.3247381/94‑1 (MQ=60)

                                                                                  CCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGAC          >  SRR4302533.5471380/1‑94 (MQ=60)

                                                                                  CCCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGAC          >  SRR4302533.2821738/1‑94 (MQ=60)

                                                                                   CCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACT         >  SRR4302533.5551341/1‑94 (MQ=60)

                                                                                   CCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACT         >  SRR4302533.1374762/1‑94 (MQ=60)

                                                                                   CCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACT         >  SRR4302533.2335870/1‑94 (MQ=60)

                                                                                   CCAGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACT         >  SRR4302533.4443402/1‑94 (MQ=60)

                                                                                     AGCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTC       >  SRR4302533.3846482/1‑94 (MQ=60)

                                                                                      GCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCC      <  SRR4302533.369418/94‑1 (MQ=60)

                                                                                      GCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCC      <  SRR4302533.3981941/94‑1 (MQ=60)

                                                                                      GCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCC      <  SRR4302533.3262516/94‑1 (MQ=60)

                                                                                      GCGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCC      <  SRR4302533.3089469/94‑1 (MQ=60)

                                                                                       CGCGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCG     <  SRR4302533.1359868/94‑1 (MQ=60)

                                                                                         CGCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCC   >  SRR4302533.3431384/1‑94 (MQ=60)

                                                                                          GCTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCA  >  SRR4302533.6198641/1‑94 (MQ=60)

AGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTGCACCACTATCAACCACGGCAACGCCGTAGTTGCTCCCATAATGCCCCAGCGCACTACGCCGCAAATCGCCGAGATCAACAGCATATCGCGTGCACTACAACGGCGGAAAAGTTTATTACTCAGCGCAAAGATAATGACTTCCGCCA  >  NC_000913/2664941‑2665124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`