Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,669,291 C→T S16L (TCG→TTG)  hcaB → 2,3‑dihydroxy‑2,3‑dihydrophenylpropionate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,669,2910CT93.6% 784.3 / 19.9 251S16L (TCG→TTG) hcaB2,3‑dihydroxy‑2,3‑dihydrophenylpropionate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (9/7);  new base T (128/107);  total (137/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 263 total aligned reads displayed.

GTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCT  >  NC_000913/2669201‑2669381
                                                                                          |                                                                                          
 ttGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTggg                                                                                         <  1:8051511/93‑1 (MQ=255)
   gAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGAt                                                                                       >  1:9391916/1‑93 (MQ=255)
    aaGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGAtt                                                                                      >  1:831235/1‑93 (MQ=255)
    aaGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGAtt                                                                                      <  1:9485882/93‑1 (MQ=255)
    aaGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGAtt                                                                                      <  1:2700679/93‑1 (MQ=255)
     aGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTa                                                                                     <  1:9542348/93‑1 (MQ=255)
     aGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTa                                                                                     >  2:3701642/1‑93 (MQ=255)
     aGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTa                                                                                     <  2:1952726/93‑1 (MQ=255)
      ggtggtGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAg                                                                                    <  2:1333128/93‑1 (MQ=255)
      ggtggtGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAg                                                                                    <  2:348449/93‑1 (MQ=255)
        tggtgACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAggg                                                                                  >  1:7424658/1‑93 (MQ=255)
         ggtgACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGc                                                                                 <  2:1556374/93‑1 (MQ=255)
          gtgACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCt                                                                                <  2:2810580/93‑1 (MQ=255)
           tgACATTTTCATCGACTTACCGGAGGGGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTAACGGCGGCGGATTGGGATTAGGGGTg                                                                               >  1:1654867/1‑93 (MQ=255)
            gACATTTTCATCGACTTACCTGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTgg                                                                              <  1:4844335/93‑1 (MQ=255)
            gACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTgg                                                                              >  2:1389210/1‑93 (MQ=255)
             aCATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATACCGAGTCCATTTTTATTACCGGCGGCGGAGTGGGATTAtggctggc                                                                             >  2:436352/1‑93 (MQ=255)
             aCATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGgc                                                                             >  2:6484632/1‑93 (MQ=255)
                ttttCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGGGCt                                                                          >  1:3208867/1‑93 (MQ=255)
                ttttCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCt                                                                          >  1:3532723/1‑93 (MQ=255)
                ttttCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCt                                                                          >  1:1447442/1‑93 (MQ=255)
                 tttCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTg                                                                         <  2:6294757/93‑1 (MQ=255)
                  ttCATCGACTTACCGGAGGCGCAACCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTgg                                                                        <  2:6936093/93‑1 (MQ=255)
                   tCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTTCCGGCGGTGGATTTGGAGTAGTTCTGGCGCTGGt                                                                       >  1:8921912/1‑93 (MQ=255)
                   tCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGt                                                                       <  1:10024964/93‑1 (MQ=255)
                    cATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTc                                                                      <  2:868983/93‑1 (MQ=255)
                     aTCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCg                                                                     >  2:1061541/1‑93 (MQ=255)
                      tCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGa                                                                    >  2:746697/1‑93 (MQ=255)
                      tCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGa                                                                    >  1:7116361/1‑93 (MQ=255)
                      tCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGa                                                                    >  1:6564860/1‑93 (MQ=255)
                       cGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAg                                                                   <  1:8422431/93‑1 (MQ=255)
                        gACTTACCGGAGGCGCAGCCATGAGCTATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGc                                                                  <  2:3597764/93‑1 (MQ=255)
                        gACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGc                                                                  >  2:7068360/1‑93 (MQ=255)
                          cTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGa                                                                >  1:679548/1‑93 (MQ=255)
                           ttACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGAt                                                               >  1:6398602/1‑93 (MQ=255)
                           ttACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGAt                                                               >  2:8138067/1‑93 (MQ=255)
                           ttACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGAt                                                               >  1:7582176/1‑93 (MQ=255)
                           ttACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGAt                                                               <  1:6061326/93‑1 (MQ=255)
                               cGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTAt                                                           <  2:2869237/93‑1 (MQ=255)
                                     cgcAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGGTTGGGATTAGGGCTGGCGCTGGTCGAGCGCTTTATCggaga                                                     >  2:4211446/1‑93 (MQ=255)
                                      gcAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCgaagaa                                                    >  1:8607574/1‑93 (MQ=255)
                                      gcAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCgaagaa                                                    <  2:3077216/93‑1 (MQ=255)
                                      gcAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGGTTAGGGCTGGCGCTGGTCGAGCGATTTATCggagaa                                                    >  1:3848084/1‑93 (MQ=255)
                                       cAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGaagaag                                                   >  2:3251152/1‑93 (MQ=255)
                                       cAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGaagaag                                                   >  2:7999296/1‑93 (MQ=255)
                                         gcaaTGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGACGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAGGgc                                                 <  2:8977530/90‑1 (MQ=255)
                                          ccATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGgcg                                                <  1:8841118/93‑1 (MQ=255)
                                           cATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGgcgg                                               >  1:5265611/1‑92 (MQ=255)
                                             tGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGgcgcgc                                             >  2:6352926/1‑93 (MQ=255)
                                              gAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGAGCTGGTCGAGCGATCTATCGAAGAAGGCGCGCa                                            >  1:9937010/1‑93 (MQ=255)
                                                gCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAgg                                          <  2:8415538/93‑1 (MQ=255)
                                                gCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAgg                                          >  1:7779066/1‑93 (MQ=255)
                                                gCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAgg                                          >  2:5331007/1‑93 (MQ=255)
                                                 cGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGt                                         <  2:4065907/93‑1 (MQ=255)
                                                 cGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGt                                         <  2:9899937/93‑1 (MQ=255)
                                                  gATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGtt                                        >  1:5336618/1‑93 (MQ=255)
                                                  gATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGtt                                        >  1:9282957/1‑93 (MQ=255)
                                                   aTCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTg                                       >  2:5021335/1‑93 (MQ=255)
                                                   aTCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGGTg                                       >  2:1470723/1‑93 (MQ=255)
                                                   aTCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGAGCAGGTTg                                       >  1:9900183/1‑93 (MQ=255)
                                                    tCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGc                                      >  1:7861969/1‑93 (MQ=255)
                                                    tCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGc                                      >  1:7191164/1‑93 (MQ=255)
                                                    tCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGc                                      >  2:6059918/1‑93 (MQ=255)
                                                    tCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGc                                      <  1:4646086/93‑1 (MQ=255)
                                                     cTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGcc                                     >  1:420851/1‑93 (MQ=255)
                                                      tGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCa                                    <  1:3019892/93‑1 (MQ=255)
                                                      tGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCa                                    >  2:1519266/1‑93 (MQ=255)
                                                      tGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCa                                    <  2:9242821/93‑1 (MQ=255)
                                                       gCATAACGAGTCCATTTTTATTGCCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCAc                                   <  2:3829184/93‑1 (MQ=255)
                                                          tAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCt                                >  1:2003880/1‑93 (MQ=255)
                                                          tAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGCTGCCACGcg                                >  1:7605459/1‑92 (MQ=255)
                                                           aaCGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTg                               <  1:2249062/93‑1 (MQ=255)
                                                            aCGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTgg                              >  2:8466068/1‑93 (MQ=255)
                                                             cGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTggc                             >  2:9472067/1‑92 (MQ=255)
                                                               aGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAAc                           <  2:4149235/93‑1 (MQ=255)
                                                                 tCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTg                         <  1:9741123/93‑1 (MQ=255)
                                                                 tCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTg                         >  1:438214/1‑93 (MQ=255)
                                                                  ccATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGt                        <  2:4951432/93‑1 (MQ=255)
                                                                  ccATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGt                        <  1:5515959/93‑1 (MQ=255)
                                                                  ccATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACACTGGAACTGt                        <  2:6734229/93‑1 (MQ=255)
                                                                    aTTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTcg                      >  2:1781496/1‑93 (MQ=255)
                                                                     tttttATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTcgg                     >  1:4545267/1‑93 (MQ=255)
                                                                     tttttATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTcgg                     <  1:6145947/93‑1 (MQ=255)
                                                                      ttttATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTcggc                    <  2:2031285/93‑1 (MQ=255)
                                                                         tattaCCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCggcggc                 >  2:2580038/1‑93 (MQ=255)
                                                                          attaCCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTTCCACGCTGGAACTGTCGGCGGCa                >  2:5534241/1‑93 (MQ=255)
                                                                          attaCCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCa                >  1:2097123/1‑93 (MQ=255)
                                                                           ttaCCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCTATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCaa               <  2:9597186/93‑1 (MQ=255)
                                                                           ttaCCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCaa               >  1:1052591/1‑93 (MQ=255)
                                                                            taCCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCaaa              >  1:3421346/1‑93 (MQ=255)
                                                                              ccGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAg            >  2:9678693/1‑93 (MQ=255)
                                                                                 gcggcggATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCg         >  1:4735381/1‑93 (MQ=255)
                                                                                   ggcggATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGcc       <  2:2475104/93‑1 (MQ=255)
                                                                                   ggcggATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGcc       >  1:5087509/1‑93 (MQ=255)
                                                                                     cggATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAg     <  1:5733553/93‑1 (MQ=255)
                                                                                      ggATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGt    >  1:582311/1‑93 (MQ=255)
                                                                                       gATTGGGATTAGGGCTGGCGCTGGTCTAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGACAGTc   >  2:4502726/1‑93 (MQ=255)
                                                                                       gATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTc   >  1:9789782/1‑93 (MQ=255)
                                                                                       gATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTc   <  1:5993102/93‑1 (MQ=255)
                                                                                        aTCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCt  <  1:4257912/93‑1 (MQ=255)
                                                                                          |                                                                                          
GTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCT  >  NC_000913/2669201‑2669381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCG  >  NC_000913/2669198‑2669384
                                                                                             |                                                                                             
CACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATT                                                                                               <  SRR4302540.3050581/94‑1 (MQ=60)
CACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATT                                                                                               >  SRR4302540.2857451/1‑94 (MQ=60)
CACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATT                                                                                               <  SRR4302540.1912937/94‑1 (MQ=60)
 ACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTG                                                                                              <  SRR4302540.7379126/94‑1 (MQ=60)
  CGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGG                                                                                             <  SRR4302540.3567174/94‑1 (MQ=60)
    TTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGA                                                                                           <  SRR4302540.8145025/94‑1 (MQ=60)
     TGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGAT                                                                                          <  SRR4302540.4507878/94‑1 (MQ=60)
     TGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGAT                                                                                          >  SRR4302540.9503677/1‑94 (MQ=60)
      GAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATT                                                                                         >  SRR4302540.840064/1‑94 (MQ=60)
       AAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTA                                                                                        <  SRR4302540.2729824/94‑1 (MQ=60)
       AAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTA                                                                                        <  SRR4302540.9598349/94‑1 (MQ=60)
       AAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTA                                                                                        <  SRR4302540.4785014/94‑1 (MQ=60)
        AGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAG                                                                                       >  SRR4302540.5420564/1‑94 (MQ=60)
        AGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAG                                                                                       <  SRR4302540.257419/94‑1 (MQ=60)
        AGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAG                                                                                       <  SRR4302540.9655257/94‑1 (MQ=60)
         GGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGG                                                                                      >  SRR4302540.5263023/1‑94 (MQ=60)
         GGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGG                                                                                      <  SRR4302540.8328814/94‑1 (MQ=60)
         GGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGG                                                                                      <  SRR4302540.3462488/94‑1 (MQ=60)
         GGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGGCCATTTTTATTACCGGCGGCGGATTGGGATTAGG                                                                                      >  SRR4302540.5773959/1‑94 (MQ=60)
          GTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGG                                                                                     >  SRR4302540.6483569/1‑94 (MQ=60)
          GTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGG                                                                                     >  SRR4302540.7507365/1‑94 (MQ=60)
             GTGACATTTTCATCGACTTACCGGAGGGGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTG                                                                                  >  SRR4302540.1672896/1‑94 (MQ=60)
             GTGACATTTTCATCGACTTACCGGAGGGGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTAACGGCGGCGGATTGGGATTAGGGGTG                                                                                  >  SRR4302540.1672897/1‑94 (MQ=60)
             GTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTG                                                                                  <  SRR4302540.9634017/94‑1 (MQ=60)
              TGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGTGGATTGGGATTAGGGCTGG                                                                                 <  SRR4302540.8727826/94‑1 (MQ=60)
               GACATTTTCATCGACTTACCTGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGC                                                                                <  SRR4302540.4896228/94‑1 (MQ=60)
                 CATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGC                                                                              >  SRR4302540.5119670/1‑94 (MQ=60)
                 CATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGC                                                                              >  SRR4302540.9380963/1‑94 (MQ=60)
                  ATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGGGCT                                                                             >  SRR4302540.3243710/1‑94 (MQ=60)
                  ATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCT                                                                             >  SRR4302540.1463180/1‑94 (MQ=60)
                  ATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCT                                                                             >  SRR4302540.1195229/1‑94 (MQ=60)
                  ATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCT                                                                             >  SRR4302540.3571285/1‑94 (MQ=60)
                  ATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCG                                                                             >  SRR4302540.2143266/1‑94 (MQ=60)
                  ATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCG                                                                             >  SRR4302540.2924115/1‑94 (MQ=60)
                    TTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGG                                                                           <  SRR4302540.4620257/94‑1 (MQ=60)
                     TTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTTCCGGCGGTGGATTTGGAGTAGTTCTGGCGCTGGT                                                                          >  SRR4302540.9025368/1‑94 (MQ=60)
                      TCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTC                                                                         <  SRR4302540.10141614/94‑1 (MQ=60)
                       CATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCG                                                                        >  SRR4302540.8448899/1‑94 (MQ=60)
                        ATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGA                                                                       >  SRR4302540.9837732/1‑94 (MQ=60)
                        ATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGA                                                                       >  SRR4302540.6637615/1‑94 (MQ=60)
                        ATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGA                                                                       >  SRR4302540.7195582/1‑94 (MQ=60)
                          CGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGC                                                                     <  SRR4302540.8520191/94‑1 (MQ=60)
                           GACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCG                                                                    <  SRR4302540.1022359/94‑1 (MQ=60)
                           GACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCG                                                                    >  SRR4302540.4407488/1‑94 (MQ=60)
                            ACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGA                                                                   >  SRR4302540.686828/1‑94 (MQ=60)
                            ACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGA                                                                   >  SRR4302540.7586694/1‑94 (MQ=60)
                             CTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGAT                                                                  >  SRR4302540.6469835/1‑94 (MQ=60)
                             CTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGAT                                                                  >  SRR4302540.7667296/1‑94 (MQ=60)
                             CTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGAT                                                                  <  SRR4302540.9890/94‑1 (MQ=60)
                             CTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGAT                                                                  >  SRR4302540.1703898/1‑94 (MQ=60)
                             CTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGAT                                                                  >  SRR4302540.2085632/1‑94 (MQ=60)
                             CTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGGCCATTTTTATTACCGGCGGCGGGTTGGGATTAGAGCTGGCGCTTGCCGAGCGAT                                                                  >  SRR4302540.7667297/1‑94 (MQ=60)
                              TTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATT                                                                 <  SRR4302540.6128692/94‑1 (MQ=60)
                               TACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTTGGATTAGGGCTGGCGCTGGTCGAGCGATTT                                                                >  SRR4302540.340598/1‑94 (MQ=60)
                               TACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTT                                                                >  SRR4302540.1387836/1‑94 (MQ=60)
                                ACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTAGAGCGATTTA                                                               >  SRR4302540.8773425/1‑94 (MQ=60)
                                 CCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTAT                                                              >  SRR4302540.9526257/1‑94 (MQ=60)
                                  CGGAGACGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATC                                                             <  SRR4302540.5231643/94‑1 (MQ=60)
                                   GGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCG                                                            <  SRR4302540.4373510/94‑1 (MQ=60)
                                     AGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAA                                                          >  SRR4302540.5949574/1‑94 (MQ=60)
                                       GCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGA                                                        <  SRR4302540.8546178/94‑1 (MQ=60)
                                       GCCCAGGCATGAGCGATCTGCATAACGAGTCCATTTTTATGACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGA                                                        <  SRR4302540.8546177/94‑1 (MQ=60)
                                        CGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAA                                                       <  SRR4302540.5844232/94‑1 (MQ=60)
                                        CGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAA                                                       >  SRR4302540.8707598/1‑94 (MQ=60)
                                        CGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAA                                                       <  SRR4302540.9123391/94‑1 (MQ=60)
                                        CGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGGTTAGGGCTGGCGCTGGTCGAGCGATTTATCGGAGAA                                                       >  SRR4302540.3889929/1‑94 (MQ=60)
                                         GCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAG                                                      >  SRR4302540.4757453/1‑94 (MQ=60)
                                           AGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGC                                                    >  SRR4302540.9874046/1‑94 (MQ=60)
                                            GCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCG                                                   <  SRR4302540.3167324/94‑1 (MQ=60)
                                            GCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCG                                                   <  SRR4302540.481307/94‑1 (MQ=60)
                                             CCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGG                                                  >  SRR4302540.5323075/1‑94 (MQ=60)
                                             CCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGC                                                  <  SRR4302540.8943380/94‑1 (MQ=60)
                                             CCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGC                                                  <  SRR4302540.10018272/94‑1 (MQ=60)
                                              CATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCG                                                 <  SRR4302540.6175295/94‑1 (MQ=60)
                                               ATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGC                                                >  SRR4302540.533141/1‑94 (MQ=60)
                                                TGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCA                                               <  SRR4302540.2029929/94‑1 (MQ=60)
                                                TGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCA                                               >  SRR4302540.9432161/1‑94 (MQ=60)
                                                TGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGAGCTGGTCGAGCGATCTATCGAAGAAGGCGCGCA                                               >  SRR4302540.10052967/1‑94 (MQ=60)
                                                  AGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGG                                             >  SRR4302540.7869999/1‑94 (MQ=60)
                                                    CGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCCGGTT                                           >  SRR4302540.6828486/1‑94 (MQ=60)
                                                    CGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTT                                           >  SRR4302540.9393905/1‑94 (MQ=60)
                                                    CGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTT                                           >  SRR4302540.6828487/1‑94 (MQ=60)
                                                    CGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTT                                           >  SRR4302540.6927818/1‑94 (MQ=60)
                                                    CGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTT                                           >  SRR4302540.5395113/1‑94 (MQ=60)
                                                    CGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTT                                           >  SRR4302540.9512002/1‑94 (MQ=60)
                                                    CGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTT                                           >  SRR4302540.7817363/1‑94 (MQ=60)
                                                     GATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTG                                          >  SRR4302540.4303016/1‑94 (MQ=60)
                                                     GATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGAGCAGGTTG                                          >  SRR4302540.10015841/1‑94 (MQ=60)
                                                      ATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGC                                         >  SRR4302540.7953826/1‑94 (MQ=60)
                                                      ATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGC                                         >  SRR4302540.5910738/1‑94 (MQ=60)
                                                      ATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGC                                         >  SRR4302540.7271319/1‑94 (MQ=60)
                                                       TCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCC                                        >  SRR4302540.425280/1‑94 (MQ=60)
                                                       TCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCC                                        <  SRR4302540.4695815/94‑1 (MQ=60)
                                                         TGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCAC                                      >  SRR4302540.6118584/1‑94 (MQ=60)
                                                         TGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCAC                                      <  SRR4302540.2760444/94‑1 (MQ=60)
                                                         TGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCAC                                      <  SRR4302540.3053084/94‑1 (MQ=60)
                                                            ATAACGAGTCCATTTTTATTACCGGCGGCGGGTTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCGACGCT                                   >  SRR4302540.9148809/1‑94 (MQ=60)
                                                            ATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCT                                   >  SRR4302540.7306597/1‑94 (MQ=60)
                                                            ATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCT                                   >  SRR4302540.2025570/1‑94 (MQ=60)
                                                            ATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCT                                   >  SRR4302540.9006411/1‑94 (MQ=60)
                                                            ATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGCTGCCACGCG                                   >  SRR4302540.7690921/1‑94 (MQ=60)
                                                              AACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGG                                 <  SRR4302540.2273210/94‑1 (MQ=60)
                                                               ACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGA                                >  SRR4302540.9954839/1‑94 (MQ=60)
                                                                 GAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAAC                              <  SRR4302540.3845745/94‑1 (MQ=60)
                                                                  AGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACT                             >  SRR4302540.7440809/1‑94 (MQ=60)
                                                                  AGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACT                             <  SRR4302540.6935282/94‑1 (MQ=60)
                                                                  AGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACT                             >  SRR4302540.7659789/1‑94 (MQ=60)
                                                                  AGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACT                             <  SRR4302540.6336048/94‑1 (MQ=60)
                                                                   GTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTG                            >  SRR4302540.442868/1‑94 (MQ=60)
                                                                   GTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTG                            >  SRR4302540.454307/1‑94 (MQ=60)
                                                                    TCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGT                           <  SRR4302540.9855470/94‑1 (MQ=60)
                                                                    TCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGT                           <  SRR4302540.3357229/94‑1 (MQ=60)
                                                                    TCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGC                           >  SRR4302540.789654/1‑94 (MQ=60)
                                                                     CCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTC                          <  SRR4302540.7202201/94‑1 (MQ=60)
                                                                     CCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTC                          <  SRR4302540.5576713/94‑1 (MQ=60)
                                                                     CCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTC                          >  SRR4302540.493946/1‑94 (MQ=60)
                                                                      CATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCG                         >  SRR4302540.3998731/1‑94 (MQ=60)
                                                                      CATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCG                         >  SRR4302540.2357015/1‑94 (MQ=60)
                                                                      CATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCG                         <  SRR4302540.810964/94‑1 (MQ=60)
                                                                       ATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGG                        <  SRR4302540.8117445/94‑1 (MQ=60)
                                                                       ATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGG                        >  SRR4302540.4594452/1‑94 (MQ=60)
                                                                        TTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGC                       <  SRR4302540.6214402/94‑1 (MQ=60)
                                                                           TTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGC                    >  SRR4302540.4116971/1‑94 (MQ=60)
                                                                            TATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCA                   >  SRR4302540.2119638/1‑94 (MQ=60)
                                                                             ATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAA                  >  SRR4302540.1063304/1‑94 (MQ=60)
                                                                              TTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAA                 >  SRR4302540.3458551/1‑94 (MQ=60)
                                                                                ACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAG               >  SRR4302540.5650256/1‑94 (MQ=60)
                                                                                  CGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGTAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTC             <  SRR4302540.629528/94‑1 (MQ=60)
                                                                                  CGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTC             <  SRR4302540.3658531/94‑1 (MQ=60)
                                                                                  CGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTC             <  SRR4302540.9629224/94‑1 (MQ=60)
                                                                                   GGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCG            >  SRR4302540.4785936/1‑94 (MQ=60)
                                                                                   GGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGCACTGTCGGCGGCAAAAGTCG            >  SRR4302540.5418177/1‑94 (MQ=60)
                                                                                    GCGGCGGATTGGGATTAGGGCTGTCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGC           <  SRR4302540.7744536/94‑1 (MQ=60)
                                                                                     CGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCC          >  SRR4302540.5142518/1‑94 (MQ=60)
                                                                                     CGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCC          <  SRR4302540.8175694/94‑1 (MQ=60)
                                                                                       GCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAG        <  SRR4302540.1689276/94‑1 (MQ=60)
                                                                                        CGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGT       <  SRR4302540.5796709/94‑1 (MQ=60)
                                                                                        CGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGT       >  SRR4302540.588607/1‑94 (MQ=60)
                                                                                        CGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGT       >  SRR4302540.9935569/1‑94 (MQ=60)
                                                                                         GGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTC      >  SRR4302540.9904466/1‑94 (MQ=60)
                                                                                          GATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCT     <  SRR4302540.6059494/94‑1 (MQ=60)
                                                                                          GATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCT     <  SRR4302540.6081195/94‑1 (MQ=60)
                                                                                          GATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCT     >  SRR4302540.7619825/1‑94 (MQ=60)
                                                                                           ATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGGCGCCAGTCTG    >  SRR4302540.3704688/1‑94 (MQ=60)
                                                                                           ATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTG    <  SRR4302540.4304232/94‑1 (MQ=60)
                                                                                             TGGGATTAGGGGTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGACCTGTCGGCGGCAAAAGTCGCCAGTCTGCG  <  SRR4302540.6915937/94‑1 (MQ=60)
                                                                                             TGGGAGTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCG  <  SRR4302540.7314685/94‑1 (MQ=60)
                                                                                             |                                                                                             
CACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCG  >  NC_000913/2669198‑2669384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑