Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,669,291 C→T S16L (TCG→TTG)  hcaB → 2,3‑dihydroxy‑2,3‑dihydrophenylpropionate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,669,2910CT100.0% 734.4 / NA 204S16L (TCG→TTG) hcaB2,3‑dihydroxy‑2,3‑dihydrophenylpropionate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (106/98);  total (106/98)

Only 100 of 217 total aligned reads displayed.

GTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTC  >  NC_000913/2669201‑2669380
                                                                                          |                                                                                         
gTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTgg                                                                                         >  1:3872147/1‑93 (MQ=255)
 ttGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTggg                                                                                        <  1:1430905/93‑1 (MQ=255)
 ttGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTggg                                                                                        <  1:5212104/93‑1 (MQ=255)
 ttGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTggg                                                                                        <  1:5258550/93‑1 (MQ=255)
  tGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGa                                                                                       >  2:459987/1‑93 (MQ=255)
    aaGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGAtt                                                                                     <  1:1899100/93‑1 (MQ=255)
    aaGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGAtt                                                                                     <  1:3282267/93‑1 (MQ=255)
     aGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTa                                                                                    >  1:4782434/1‑93 (MQ=255)
      ggtgttAACATTTTCATCGGCTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAg                                                                                   <  2:3254457/93‑1 (MQ=255)
      ggtggtGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAg                                                                                   <  2:1166565/93‑1 (MQ=255)
      ggtggtGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAg                                                                                   <  2:2148785/93‑1 (MQ=255)
       gtggtgACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGTGGATTGGGATTAgg                                                                                  <  2:3579910/93‑1 (MQ=255)
         ggtgACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGc                                                                                >  1:2622135/1‑93 (MQ=255)
         ggtgACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGc                                                                                >  2:6261310/1‑93 (MQ=255)
          gtgACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCt                                                                               >  1:5530318/1‑93 (MQ=255)
           tgACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTg                                                                              >  1:3172361/1‑93 (MQ=255)
           tgACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTg                                                                              >  2:2606247/1‑93 (MQ=255)
            gACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTgg                                                                             <  1:5974543/93‑1 (MQ=255)
            gACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTgg                                                                             >  1:3673951/1‑93 (MQ=255)
                ttttCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCt                                                                         <  1:1300767/93‑1 (MQ=255)
                ttttCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCt                                                                         >  1:2521206/1‑93 (MQ=255)
                ttttCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCt                                                                         >  1:1883585/1‑93 (MQ=255)
                  ttCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTgg                                                                       <  2:380306/93‑1 (MQ=255)
                   tCATCGCCTTACCGGAGGCGCAGCCAGGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGt                                                                      <  2:189720/93‑1 (MQ=255)
                    cATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGGTTGGGGTTATGGCTGGGGCTGGtg                                                                     >  1:2660826/1‑92 (MQ=255)
                    cATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTc                                                                     <  2:5569260/93‑1 (MQ=255)
                     aTCGACTTCCCGGAGGCGCAGCCATGAGCGATCTGCATAACTAGTCCATTTTTATTACCGGCGGCGAATTGGGATTAGGGCTGGCGCTGGTCg                                                                    <  1:4006356/93‑1 (MQ=255)
                     aTCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGGGCTGGTCg                                                                    >  2:2062271/1‑93 (MQ=255)
                      tCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGa                                                                   <  2:1962881/93‑1 (MQ=255)
                       cGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAg                                                                  <  2:2237819/93‑1 (MQ=255)
                       cGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAg                                                                  >  2:731904/1‑93 (MQ=255)
                        gACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGc                                                                 >  1:4352676/1‑93 (MQ=255)
                        gACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGc                                                                 >  1:5305222/1‑93 (MQ=255)
                        gACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGc                                                                 >  2:4065136/1‑93 (MQ=255)
                              ccGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTa                                                           <  1:4854611/93‑1 (MQ=255)
                               cGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTAt                                                          >  1:3553726/1‑93 (MQ=255)
                                ggAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATc                                                         >  2:4388615/1‑93 (MQ=255)
                                ggAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATc                                                         <  1:2785478/93‑1 (MQ=255)
                                ggAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGGCGAGCGATTTATc                                                         >  2:5512714/1‑93 (MQ=255)
                                 gAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCg                                                        <  2:1448905/93‑1 (MQ=255)
                                 gAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCg                                                        >  2:3302544/1‑93 (MQ=255)
                                   ggCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCgaa                                                      <  2:2389926/93‑1 (MQ=255)
                                   ggCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCgaa                                                      >  2:2689238/1‑93 (MQ=255)
                                      gcAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCgaagaa                                                   <  1:3525930/93‑1 (MQ=255)
                                      gcAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCgaagaa                                                   <  1:5074574/93‑1 (MQ=255)
                                       cAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGaagaag                                                  <  2:3113362/93‑1 (MQ=255)
                                        aGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAgg                                                 <  1:1009989/93‑1 (MQ=255)
                                         gCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAggg                                                >  1:1676569/1‑92 (MQ=255)
                                         gCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGgc                                                >  2:290176/1‑93 (MQ=255)
                                         gCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGgc                                                <  1:3367091/93‑1 (MQ=255)
                                         accATGAGCGATCTGCATAACGAGTCCATTTTTATTGCCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGgc                                                <  1:5935364/92‑1 (MQ=255)
                                          ccATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAgggg                                               >  2:4490389/1‑91 (MQ=255)
                                           cATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGgcgc                                              >  2:4918787/1‑93 (MQ=255)
                                           cATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGgcgc                                              <  2:6109862/93‑1 (MQ=255)
                                            cggAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGgcgcg                                             <  2:593535/91‑1 (MQ=255)
                                              gAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCa                                           <  1:1259342/93‑1 (MQ=255)
                                              gAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCa                                           <  2:1326369/93‑1 (MQ=255)
                                              gAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCa                                           <  2:4998724/93‑1 (MQ=255)
                                               aGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAg                                          <  2:3198263/93‑1 (MQ=255)
                                                gCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAgg                                         <  1:5349122/93‑1 (MQ=255)
                                                   aTCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTg                                      >  2:5170239/1‑93 (MQ=255)
                                                   aTCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTg                                      >  1:6077033/1‑93 (MQ=255)
                                                      tGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCa                                   >  1:3773558/1‑93 (MQ=255)
                                                       gCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCAc                                  >  1:73884/1‑93 (MQ=255)
                                                        cATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACg                                 <  1:2315131/93‑1 (MQ=255)
                                                         aTAACGAGTCCATTTTTATTACCGGCCGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGc                                <  2:5091544/93‑1 (MQ=255)
                                                          tAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCt                               >  2:1030156/1‑93 (MQ=255)
                                                          tAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCt                               <  2:1256675/93‑1 (MQ=255)
                                                            aCGAGTCCATTTTTTTTAACGGCGGCGGGTTGGGGTTTGGGGTTGGGCTGGGCGGGAGGTTTATCGGAGAAAGGGCGCCGGGTGCCACGcccg                             >  2:3705731/1‑90 (MQ=255)
                                                               aGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAAc                          <  1:1154168/93‑1 (MQ=255)
                                                               aGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAAc                          >  1:5388261/1‑93 (MQ=255)
                                                                gTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACt                         >  1:2073336/1‑93 (MQ=255)
                                                                gTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACt                         >  2:5047525/1‑93 (MQ=255)
                                                                  ccATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGt                       <  2:2644282/93‑1 (MQ=255)
                                                                   cATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTc                      <  2:2981472/93‑1 (MQ=255)
                                                                   cATTTTGATTACCGGCGGCGGATTGGGATGAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTc                      <  2:1988422/93‑1 (MQ=255)
                                                                    aTTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTcg                     <  2:2846036/93‑1 (MQ=255)
                                                                    aTTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTcg                     >  1:2958476/1‑93 (MQ=255)
                                                                    aTTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTcg                     <  1:3742055/93‑1 (MQ=255)
                                                                    aTTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTcg                     <  1:5453694/93‑1 (MQ=255)
                                                                    aTTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTcg                     <  1:563158/93‑1 (MQ=255)
                                                                     tttttATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTcgg                    <  2:6177502/93‑1 (MQ=255)
                                                                     tttttATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACGGTcgg                    <  2:5799315/93‑1 (MQ=255)
                                                                       tttATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTcggcg                  >  2:4751912/1‑93 (MQ=255)
                                                                          attaCCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCa               <  1:4613742/93‑1 (MQ=255)
                                                                           ttaCCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCaa              >  1:4045132/1‑93 (MQ=255)
                                                                            taCCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCaaa             >  2:5766922/1‑93 (MQ=255)
                                                                             aCCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCaaaa            <  1:5802109/93‑1 (MQ=255)
                                                                             aCCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGCCGGCGGCacaa            >  2:4115751/1‑93 (MQ=255)
                                                                              ccGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAg           >  2:1187770/1‑93 (MQ=255)
                                                                               cggcggcggATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGt          <  2:3349179/93‑1 (MQ=255)
                                                                                ggcggcggATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTc         <  1:4388750/93‑1 (MQ=255)
                                                                                 gcggcggATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCg        <  2:2522013/93‑1 (MQ=255)
                                                                                  cggcggATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGc       <  1:2448003/93‑1 (MQ=255)
                                                                                       gATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTc  >  2:1748462/1‑93 (MQ=255)
                                                                                       gATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTc  >  2:4230913/1‑93 (MQ=255)
                                                                                       gATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTc  <  2:3920338/93‑1 (MQ=255)
                                                                                       gATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTc  >  1:6242213/1‑93 (MQ=255)
                                                                                       gATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTc  >  2:853329/1‑93 (MQ=255)
                                                                                       gATTGGGATTAGGGCTGGCGATGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTc  >  2:6064372/1‑93 (MQ=255)
                                                                                          |                                                                                         
GTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTC  >  NC_000913/2669201‑2669380

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCG  >  NC_000913/2669200‑2669384
                                                                                           |                                                                                             
CGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGG                                                                                             >  SRR4302533.3895780/1‑94 (MQ=60)
CGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGG                                                                                             <  SRR4302533.3082879/94‑1 (MQ=60)
  TTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGA                                                                                           <  SRR4302533.5243492/94‑1 (MQ=60)
  TTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGA                                                                                           <  SRR4302533.5290124/94‑1 (MQ=60)
  TTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGA                                                                                           >  SRR4302533.3798813/1‑94 (MQ=60)
  TTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGA                                                                                           <  SRR4302533.1439554/94‑1 (MQ=60)
     AAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTA                                                                                        <  SRR4302533.1910696/94‑1 (MQ=60)
     AAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTA                                                                                        >  SRR4302533.4811409/1‑94 (MQ=60)
     AAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTA                                                                                        <  SRR4302533.3302531/94‑1 (MQ=60)
      AGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAG                                                                                       <  SRR4302533.2342092/94‑1 (MQ=60)
       GGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGG                                                                                      >  SRR4302533.6038772/1‑94 (MQ=60)
        GTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGG                                                                                     >  SRR4302533.4820249/1‑94 (MQ=60)
         TGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGC                                                                                    >  SRR4302533.2638230/1‑94 (MQ=60)
          GGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCT                                                                                   >  SRR4302533.5563704/1‑94 (MQ=60)
          GGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCT                                                                                   <  SRR4302533.1380471/94‑1 (MQ=60)
           GTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTG                                                                                  >  SRR4302533.3191891/1‑94 (MQ=60)
            TGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGG                                                                                 >  SRR4302533.3696531/1‑94 (MQ=60)
            TGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGG                                                                                 >  SRR4302533.3193596/1‑94 (MQ=60)
             GACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGC                                                                                <  SRR4302533.3435394/94‑1 (MQ=60)
             GACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGC                                                                                <  SRR4302533.6010705/94‑1 (MQ=60)
                ATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCT                                                                             >  SRR4302533.3972426/1‑94 (MQ=60)
                ATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCT                                                                             >  SRR4302533.1895088/1‑94 (MQ=60)
                ATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCT                                                                             >  SRR4302533.2536740/1‑94 (MQ=60)
                 TTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTG                                                                            <  SRR4302533.1308591/94‑1 (MQ=60)
                     CATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCG                                                                        <  SRR4302533.2072724/94‑1 (MQ=60)
                     CATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCG                                                                        >  SRR4302533.1273651/1‑94 (MQ=60)
                      ATCGACTTCCCGGAGGCGCAGCCATGAGCGATCTGCATAACTAGTCCATTTTTATTACCGGCGGCGAATTGGGATTAGGGCTGGCGCTGGTCGA                                                                       <  SRR4302533.4030747/94‑1 (MQ=60)
                      ATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGG                                                                       >  SRR4302533.5986274/1‑94 (MQ=60)
                      ATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGA                                                                       >  SRR4302533.3768947/1‑94 (MQ=60)
                        CGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGC                                                                     >  SRR4302533.5337016/1‑94 (MQ=60)
                        CGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGC                                                                     >  SRR4302533.4378942/1‑94 (MQ=60)
                          ACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGA                                                                   <  SRR4302533.5235220/94‑1 (MQ=60)
                          ACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGA                                                                   >  SRR4302533.3100362/1‑94 (MQ=60)
                             TACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTT                                                                >  SRR4302533.4227694/1‑94 (MQ=60)
                              ACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTA                                                               >  SRR4302533.4648838/1‑94 (MQ=60)
                               CCGGAGGCGCAGCCATGAGCTATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTAT                                                              <  SRR4302533.5267574/94‑1 (MQ=60)
                               CCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTAT                                                              >  SRR4302533.3575621/1‑94 (MQ=60)
                               CCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTAT                                                              <  SRR4302533.4884030/94‑1 (MQ=60)
                                CGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATC                                                             >  SRR4302533.2536319/1‑94 (MQ=60)
                                CGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATC                                                             >  SRR4302533.5707984/1‑94 (MQ=60)
                                CGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATC                                                             <  SRR4302533.5405657/94‑1 (MQ=60)
                                 GGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCG                                                            >  SRR4302533.2824627/1‑94 (MQ=60)
                                 GGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCG                                                            <  SRR4302533.2802593/94‑1 (MQ=60)
                                 GGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCG                                                            <  SRR4302533.2609542/94‑1 (MQ=60)
                                  GAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGA                                                           >  SRR4302533.2214456/1‑94 (MQ=60)
                                    GGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTGTTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAG                                                         <  SRR4302533.1813719/94‑1 (MQ=60)
                                     GCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGA                                                        <  SRR4302533.3801912/94‑1 (MQ=60)
                                       GCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAG                                                      <  SRR4302533.3547656/94‑1 (MQ=60)
                                       GCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAG                                                      <  SRR4302533.5105248/94‑1 (MQ=60)
                                        CAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGG                                                     >  SRR4302533.3750099/1‑94 (MQ=60)
                                         AGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGG                                                    >  SRR4302533.1686721/1‑94 (MQ=60)
                                         AGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGC                                                    >  SRR4302533.686065/1‑94 (MQ=60)
                                         AGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGC                                                    <  SRR4302533.1015895/94‑1 (MQ=60)
                                         AGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGC                                                    >  SRR4302533.3686034/1‑94 (MQ=60)
                                          GCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCG                                                   <  SRR4302533.3387888/94‑1 (MQ=60)
                                          ACCATGAGCGATCTGCATAACGAGTCCATTTTTATTGCCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCG                                                   <  SRR4302533.5971246/94‑1 (MQ=60)
                                            CATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCG                                                 <  SRR4302533.1694257/94‑1 (MQ=60)
                                            CATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCG                                                 >  SRR4302533.4395333/1‑94 (MQ=60)
                                             ATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGGGCGC                                                >  SRR4302533.6198849/1‑94 (MQ=60)
                                               GAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAG                                              <  SRR4302533.1266873/94‑1 (MQ=60)
                                                AGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGG                                             <  SRR4302533.3558500/94‑1 (MQ=60)
                                                 GCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGT                                            <  SRR4302533.5381214/94‑1 (MQ=60)
                                                 GCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGT                                            >  SRR4302533.4617157/1‑94 (MQ=60)
                                                   GATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTG                                          <  SRR4302533.1909601/94‑1 (MQ=60)
                                                   GATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTG                                          >  SRR4302533.6113786/1‑94 (MQ=60)
                                                     cgatctgctgtatTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCC                                        <  SRR4302533.4985076/81‑1 (MQ=60)
                                                      CTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCA                                       >  SRR4302533.3796690/1‑94 (MQ=60)
                                                       TGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCAC                                      >  SRR4302533.2570388/1‑94 (MQ=60)
                                                       TGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCAC                                      >  SRR4302533.4057931/1‑94 (MQ=60)
                                                       TGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCAC                                      >  SRR4302533.74360/1‑94 (MQ=60)
                                                       TGCATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCAC                                      >  SRR4302533.2712070/1‑94 (MQ=60)
                                                         CATAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGC                                    <  SRR4302533.2329470/94‑1 (MQ=60)
                                                           TAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGCGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTG                                  >  SRR4302533.5567687/1‑94 (MQ=60)
                                                           TAACGAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTG                                  <  SRR4302533.5316911/94‑1 (MQ=60)
                                                               GAGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAAC                              >  SRR4302533.5420639/1‑94 (MQ=60)
                                                                AGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACT                             >  SRR4302533.1124203/1‑94 (MQ=60)
                                                                AGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACT                             <  SRR4302533.1161086/94‑1 (MQ=60)
                                                                AGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACT                             >  SRR4302533.2086105/1‑94 (MQ=60)
                                                                AGTCCATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACT                             >  SRR4302533.3304102/1‑94 (MQ=60)
                                                                    CATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCG                         >  SRR4302533.2976559/1‑94 (MQ=60)
                                                                     ATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGG                        <  SRR4302533.566496/94‑1 (MQ=60)
                                                                     ATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGG                        <  SRR4302533.3765004/94‑1 (MQ=60)
                                                                     ATTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGG                        <  SRR4302533.5486544/94‑1 (MQ=60)
                                                                      TTTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGC                       <  SRR4302533.5526972/94‑1 (MQ=60)
                                                                       TTTTATTACCGGCGGCGGATTGGGATTAGGGCTGGCCCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCG                      <  SRR4302533.5379231/94‑1 (MQ=60)
                                                                           ATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAA                  <  SRR4302533.4641690/94‑1 (MQ=60)
                                                                           ATTACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAA                  >  SRR4302533.4069752/1‑94 (MQ=60)
                                                                              ACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAG               <  SRR4302533.4801793/94‑1 (MQ=60)
                                                                              ACCGGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAG               <  SRR4302533.5837157/94‑1 (MQ=60)
                                                                                 GGCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCG            <  SRR4302533.4415248/94‑1 (MQ=60)
                                                                                  GCGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGCAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGC           >  SRR4302533.935513/1‑94 (MQ=60)
                                                                                   CGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCC          >  SRR4302533.5367343/1‑94 (MQ=60)
                                                                                   CGGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCC          <  SRR4302533.2463111/94‑1 (MQ=60)
                                                                                    GGGGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTGATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCA         <  SRR4302533.589670/94‑1 (MQ=60)
                                                                                    GGCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCA         <  SRR4302533.1663314/94‑1 (MQ=60)
                                                                                     GCGGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAG        <  SRR4302533.1261819/94‑1 (MQ=60)
                                                                                       GGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTC      >  SRR4302533.5430894/1‑94 (MQ=60)
                                                                                       GGATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTC      >  SRR4302533.6279582/1‑94 (MQ=60)
                                                                                        GATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCT     <  SRR4302533.3344215/94‑1 (MQ=60)
                                                                                        GATTGGGACTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCT     <  SRR4302533.5842667/94‑1 (MQ=60)
                                                                                         ATTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTG    <  SRR4302533.3021319/94‑1 (MQ=60)
                                                                                          TTGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGC   <  SRR4302533.1750979/94‑1 (MQ=60)
                                                                                           TGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCG  >  SRR4302533.2880055/1‑94 (MQ=60)
                                                                                           TGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCG  >  SRR4302533.2952964/1‑94 (MQ=60)
                                                                                           TGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCG  >  SRR4302533.4175285/1‑94 (MQ=60)
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CGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTATCGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCG  >  NC_000913/2669200‑2669384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑