Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,711,991 T→C L359P (CTG→CCG)  srmB → ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,711,9910TC100.0% 933.7 / NA 284L359P (CTG→CCG) srmBATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (132/152);  total (132/152)

Only 100 of 306 total aligned reads displayed.

GCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAA  >  NC_000913/2711901‑2712080
                                                                                          |                                                                                         
gCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATctgccgc                                                                                         >  2:173478/1‑93 (MQ=255)
  gATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATctgccgctg                                                                                       >  1:3948932/1‑93 (MQ=255)
  gATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATctgccgctg                                                                                       >  1:3191273/1‑93 (MQ=255)
   aTACTTATTTGCCCCGTTGCGGACGTAACGCGAGAGTAGGTCGTAAAGTCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCggg                                                                                      <  2:9078374/93‑1 (MQ=255)
   aTACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTgg                                                                                      >  2:8374918/1‑93 (MQ=255)
    tACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGGCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTggg                                                                                     >  2:2804072/1‑93 (MQ=255)
      cTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTa                                                                                   <  2:710513/93‑1 (MQ=255)
       ttATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTaa                                                                                  <  1:83760/93‑1 (MQ=255)
         aTTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAg                                                                                <  2:6664836/93‑1 (MQ=255)
         aTTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAg                                                                                <  1:9810532/93‑1 (MQ=255)
         aTTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAg                                                                                <  1:6236454/93‑1 (MQ=255)
          tttGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGt                                                                               <  2:2014388/93‑1 (MQ=255)
           ttGCGCCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTa                                                                              <  1:2717891/93‑1 (MQ=255)
           ttGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTa                                                                              <  1:3433597/93‑1 (MQ=255)
           tggCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTa                                                                              <  1:4267572/91‑1 (MQ=255)
            tGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAg                                                                             <  1:4807271/93‑1 (MQ=255)
            tGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAg                                                                             >  1:5488264/1‑93 (MQ=255)
             gCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAgg                                                                            >  2:6433057/1‑93 (MQ=255)
              cACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGc                                                                           <  2:3872943/93‑1 (MQ=255)
               aCCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGcc                                                                          >  2:7718939/1‑93 (MQ=255)
               aCCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGcc                                                                          <  2:7448853/93‑1 (MQ=255)
                ccGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCg                                                                         <  1:5727247/93‑1 (MQ=255)
                 cGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGc                                                                        >  1:7182371/1‑93 (MQ=255)
                  gTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCt                                                                       >  2:3360286/1‑93 (MQ=255)
                  gTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCt                                                                       >  2:2731837/1‑93 (MQ=255)
                   tATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCta                                                                      <  2:4625872/93‑1 (MQ=255)
                   tATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGAGCAAGCCCATGGCCATCTGCCACTGGGCAAAGTAGGCCGCta                                                                      >  2:1803191/1‑93 (MQ=255)
                    aTCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCtat                                                                     <  2:8440188/93‑1 (MQ=255)
                     tCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCtata                                                                    <  1:6940252/93‑1 (MQ=255)
                      cGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTatat                                                                   >  2:6107648/1‑93 (MQ=255)
                       ggACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATAtt                                                                  <  1:2036225/93‑1 (MQ=255)
                         aCGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGa                                                                <  1:1490443/93‑1 (MQ=255)
                           gTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAag                                                              >  1:6302765/1‑93 (MQ=255)
                           gTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAag                                                              <  1:3721613/93‑1 (MQ=255)
                            tACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAaga                                                             <  1:8584571/93‑1 (MQ=255)
                            tACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAaga                                                             >  2:2514443/1‑93 (MQ=255)
                            tACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAaga                                                             >  2:8053566/1‑93 (MQ=255)
                             aCCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAagag                                                            >  1:4995818/1‑93 (MQ=255)
                              ccGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGc                                                           <  1:5530567/93‑1 (MQ=255)
                                gcgcgcgcCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCa                                                         <  2:234711/93‑1 (MQ=255)
                                 cgcgcgcCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCaa                                                        >  2:9572958/1‑93 (MQ=255)
                                 cgcgcgcCGGTCGTAAAGGCACCGCAATTTCGCTGGTCGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCaa                                                        >  2:8914320/1‑93 (MQ=255)
                                    gcgcCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTa                                                     >  2:2245129/1‑93 (MQ=255)
                                    gcgcCGGTCGTAAAGGCACCGCAATTCCGCTCGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTa                                                     <  1:3249389/93‑1 (MQ=255)
                                     cgcCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATaaa                                                    <  2:10016561/93‑1 (MQ=255)
                                     cgcCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTaa                                                    >  2:1161318/1‑93 (MQ=255)
                                       ccGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGTCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAg                                                  >  1:377740/1‑93 (MQ=255)
                                       ccGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAg                                                  >  2:2984096/1‑93 (MQ=255)
                                       ccGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAg                                                  >  2:5792102/1‑93 (MQ=255)
                                       ccGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAg                                                  <  2:7565558/93‑1 (MQ=255)
                                         ggTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCt                                                >  2:1321157/1‑93 (MQ=255)
                                         ggTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCt                                                >  1:640081/1‑93 (MQ=255)
                                          gTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTc                                               >  1:4719220/1‑93 (MQ=255)
                                             gTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCGGCTATATTGAAAAGCCAATTAAAGATctct                                            >  1:6664721/1‑90 (MQ=255)
                                             gTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTcgcg                                            >  2:8543675/1‑93 (MQ=255)
                                               aaaGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGtt                                          <  2:6984293/93‑1 (MQ=255)
                                               aaaGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGtt                                          >  1:7686170/1‑93 (MQ=255)
                                                aaGGCCCCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTa                                         <  2:1929357/93‑1 (MQ=255)
                                                aaGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTa                                         <  1:9436972/93‑1 (MQ=255)
                                                 aGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTAt                                        <  2:788420/93‑1 (MQ=255)
                                                   gCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATtg                                      >  1:6813079/1‑93 (MQ=255)
                                                   gCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATtg                                      <  2:631901/93‑1 (MQ=255)
                                                    cACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATtga                                     <  1:9355152/93‑1 (MQ=255)
                                                     aCCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATtgat                                    <  2:5023975/93‑1 (MQ=255)
                                                      ccGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATtgatg                                   >  2:6928578/1‑93 (MQ=255)
                                                      ccGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATtgatg                                   >  1:2943160/1‑93 (MQ=255)
                                                       cGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATtgatga                                  <  2:6850424/93‑1 (MQ=255)
                                                       cGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATGGAAGAGCCAATTAAAGCTCGCGTTATtgatga                                  <  1:1578355/93‑1 (MQ=255)
                                                        gCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAg                                 >  2:6585199/1‑93 (MQ=255)
                                                         cAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGt                                <  1:604671/93‑1 (MQ=255)
                                                         cAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGt                                <  1:10027364/93‑1 (MQ=255)
                                                         cAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGt                                <  2:592289/93‑1 (MQ=255)
                                                           aTTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTa                              <  1:4659030/93‑1 (MQ=255)
                                                            tttCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTAc                             >  1:4045425/1‑93 (MQ=255)
                                                            tttCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTAc                             <  1:7444519/93‑1 (MQ=255)
                                                             ttCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACg                            <  2:5289475/93‑1 (MQ=255)
                                                               cGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGcc                          <  1:5139101/93‑1 (MQ=255)
                                                                gCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGccc                         >  2:1633566/1‑93 (MQ=255)
                                                                gCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGccc                         <  2:6003415/93‑1 (MQ=255)
                                                                gCTGGTGGAAGCCCATGACCATCTGCCGCTGGGGAAAGTAGGCCGCTATATTGAAGACCCAATTAAAGCTCGCGTTATTGATGAGTTATGccc                         >  1:7786105/1‑93 (MQ=255)
                                                                 cTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTCCGCCCg                        >  1:8802847/1‑93 (MQ=255)
                                                                  tggtggAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGa                       >  1:231641/1‑93 (MQ=255)
                                                                    gtggAAGCCCATGACCATCTGGCGCTGGGTAAACTAGTCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGaaa                     <  1:1403278/93‑1 (MQ=255)
                                                                     tggAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGaaaa                    <  2:5527641/93‑1 (MQ=255)
                                                                           cccATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTg              <  1:7047698/93‑1 (MQ=255)
                                                                           cccATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTg              <  1:2606783/93‑1 (MQ=255)
                                                                            ccATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTgc             <  1:971723/93‑1 (MQ=255)
                                                                            ccATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTgc             <  1:9054276/93‑1 (MQ=255)
                                                                            ccATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTgc             <  1:4377471/93‑1 (MQ=255)
                                                                            ccATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTgc             <  1:10046232/93‑1 (MQ=255)
                                                                             cATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTgcg            >  2:7310225/1‑93 (MQ=255)
                                                                             cATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTgcg            <  2:3595360/93‑1 (MQ=255)
                                                                               tGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTgcgcg          >  2:2901152/1‑92 (MQ=255)
                                                                                 aCCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCaa        >  2:5336064/1‑93 (MQ=255)
                                                                                 aCCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGATCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCac        >  2:5656700/1‑92 (MQ=255)
                                                                                  ccATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAg       >  2:7345790/1‑93 (MQ=255)
                                                                                  ccATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAg       <  2:4035526/93‑1 (MQ=255)
                                                                                     tCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGa    >  2:4415723/1‑93 (MQ=255)
                                                                                      ctgccgctgGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGaa   <  1:9168819/93‑1 (MQ=255)
                                                                                       tgccgctgGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGaaa  <  2:7744368/93‑1 (MQ=255)
                                                                                          |                                                                                         
GCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAA  >  NC_000913/2711901‑2712080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCA  >  NC_000913/2711898‑2712084
                                                                                             |                                                                                             
GTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCC                                                                                               >  SRR4302540.8671457/1‑94 (MQ=60)
    CGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTG                                                                                           >  SRR4302540.3991570/1‑94 (MQ=60)
    CGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTG                                                                                           >  SRR4302540.3225937/1‑94 (MQ=60)
    CGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTG                                                                                           >  SRR4302540.2403973/1‑94 (MQ=60)
    CGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTG                                                                                           >  SRR4302540.233336/1‑94 (MQ=60)
       TACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGT                                                                                        <  SRR4302540.3168656/94‑1 (MQ=60)
         CTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAA                                                                                      >  SRR4302540.4998596/1‑94 (MQ=60)
         CTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAA                                                                                      >  SRR4302540.2657838/1‑94 (MQ=60)
         CTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAA                                                                                      >  SRR4302540.7044313/1‑94 (MQ=60)
          TTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAA                                                                                     <  SRR4302540.84642/94‑1 (MQ=60)
           TATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAG                                                                                    >  SRR4302540.9271523/1‑94 (MQ=60)
            ATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGT                                                                                   >  SRR4302540.1559097/1‑94 (MQ=60)
            ATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGT                                                                                   <  SRR4302540.3016774/94‑1 (MQ=60)
            ATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGT                                                                                   <  SRR4302540.9925347/94‑1 (MQ=60)
            ATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGT                                                                                   <  SRR4302540.6305823/94‑1 (MQ=60)
            ATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGA                                                                                   >  SRR4302540.7755230/1‑94 (MQ=60)
             TTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTA                                                                                  <  SRR4302540.9918179/94‑1 (MQ=60)
             TTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTA                                                                                  <  SRR4302540.4784393/94‑1 (MQ=60)
              TTGCGCCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAG                                                                                 <  SRR4302540.2747196/94‑1 (MQ=60)
              TTGCCCCGTGTCGGGCGTCCCGCGCGCGCCGGTCGTAACGGCCCCGCCATTTCGCTGGTGGCAGCCCATGCCCATCTGCCGCTGGGTCAAGTAG                                                                                 <  SRR4302540.7815449/94‑1 (MQ=34)
              TTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAG                                                                                 <  SRR4302540.3470925/94‑1 (MQ=60)
              TTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAG                                                                                 <  SRR4302540.339563/94‑1 (MQ=60)
              TTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAG                                                                                 >  SRR4302540.5548681/1‑94 (MQ=60)
              TGGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAG                                                                                 <  SRR4302540.4313988/94‑1 (MQ=60)
               TGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGG                                                                                <  SRR4302540.4858723/94‑1 (MQ=60)
                GCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGC                                                                               >  SRR4302540.4506896/1‑94 (MQ=60)
                GCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGC                                                                               <  SRR4302540.7251848/94‑1 (MQ=60)
                 CACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCC                                                                              >  SRR4302540.8798514/1‑94 (MQ=60)
                 CACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCC                                                                              >  SRR4302540.8798515/1‑94 (MQ=60)
                   CCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGC                                                                            <  SRR4302540.5790317/94‑1 (MQ=60)
                   CCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGC                                                                            >  SRR4302540.7262445/1‑94 (MQ=60)
                    CGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCT                                                                           <  SRR4302540.9780596/94‑1 (MQ=60)
                     GTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTA                                                                          >  SRR4302540.6983014/1‑94 (MQ=60)
                     GTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTA                                                                          >  SRR4302540.5657327/1‑94 (MQ=60)
                      TATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTAT                                                                         <  SRR4302540.6208126/94‑1 (MQ=60)
                       tgCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATA                                                                        <  SRR4302540.1014049/92‑1 (MQ=60)
                       ATCTGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATA                                                                        <  SRR4302540.1014048/94‑1 (MQ=60)
                       ATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATA                                                                        >  SRR4302540.5451650/1‑94 (MQ=60)
                        TCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATAT                                                                       <  SRR4302540.5581878/94‑1 (MQ=60)
                        TCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATAT                                                                       <  SRR4302540.5581879/94‑1 (MQ=60)
                        TCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATAT                                                                       >  SRR4302540.3660944/1‑94 (MQ=60)
                        TCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATAT                                                                       >  SRR4302540.3767177/1‑94 (MQ=60)
                        TCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATAT                                                                       <  SRR4302540.7017506/94‑1 (MQ=60)
                        TCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATAT                                                                       >  SRR4302540.4363367/1‑94 (MQ=60)
                         CGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATT                                                                      >  SRR4302540.434503/1‑94 (MQ=60)
                          GGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTG                                                                     >  SRR4302540.995282/1‑94 (MQ=60)
                          GGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTG                                                                     <  SRR4302540.4709953/94‑1 (MQ=60)
                          GGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTG                                                                     <  SRR4302540.2058182/94‑1 (MQ=60)
                            GCGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAA                                                                   <  SRR4302540.6956444/94‑1 (MQ=60)
                            ACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAA                                                                   <  SRR4302540.1506830/94‑1 (MQ=60)
                            ACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAA                                                                   <  SRR4302540.1506831/94‑1 (MQ=60)
                            ACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAA                                                                   <  SRR4302540.8613988/94‑1 (MQ=60)
                            ACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAA                                                                   <  SRR4302540.63219/94‑1 (MQ=60)
                             CGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAG                                                                  <  SRR4302540.711323/94‑1 (MQ=60)
                             CGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAG                                                                  >  SRR4302540.6372953/1‑94 (MQ=60)
                             CGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAG                                                                  <  SRR4302540.4438270/94‑1 (MQ=60)
                             CGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAG                                                                  >  SRR4302540.9500772/1‑94 (MQ=60)
                             CGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAG                                                                  >  SRR4302540.9500774/1‑94 (MQ=60)
                              GTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGA                                                                 <  SRR4302540.6760423/94‑1 (MQ=60)
                              GTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGA                                                                 <  SRR4302540.1501485/94‑1 (MQ=60)
                              GTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGA                                                                 <  SRR4302540.327757/94‑1 (MQ=60)
                              GTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGA                                                                 <  SRR4302540.4931115/94‑1 (MQ=60)
                              GTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGA                                                                 <  SRR4302540.3762217/94‑1 (MQ=60)
                               TACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAG                                                                <  SRR4302540.8684370/94‑1 (MQ=60)
                               TACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAG                                                                >  SRR4302540.5049901/1‑94 (MQ=60)
                                 CCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCC                                                              >  SRR4302540.269273/1‑94 (MQ=60)
                                 CCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCC                                                              <  SRR4302540.5591477/94‑1 (MQ=60)
                                 CCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCC                                                              <  SRR4302540.9436025/94‑1 (MQ=60)
                                   GCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAA                                                            >  SRR4302540.6482977/1‑94 (MQ=60)
                                     GCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCACCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATT                                                          <  SRR4302540.6726361/94‑1 (MQ=60)
                                       GCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAA                                                        <  SRR4302540.3284782/94‑1 (MQ=60)
                                       GCGCCGGTCGTAAAGGCACCGCAATTCCGCTCGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAA                                                        <  SRR4302540.3284781/94‑1 (MQ=60)
                                         GCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGTCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAG                                                      >  SRR4302540.381645/1‑94 (MQ=60)
                                         GCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAG                                                      <  SRR4302540.4114301/94‑1 (MQ=60)
                                         GCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAG                                                      >  SRR4302540.6406296/1‑94 (MQ=60)
                                           CGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCTGCTATATTGAAGAGCCAATTAAAGCT                                                    >  SRR4302540.3237632/1‑94 (MQ=60)
                                           CGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCT                                                    >  SRR4302540.646900/1‑94 (MQ=60)
                                            GGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTC                                                   >  SRR4302540.4769627/1‑94 (MQ=60)
                                              TCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGC                                                 <  SRR4302540.2959513/94‑1 (MQ=60)
                                              TCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGC                                                 <  SRR4302540.570766/94‑1 (MQ=60)
                                               CGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCGGCTATATTGAAAAGCCAATTAAAGatctct                                                >  SRR4302540.6738390/1‑88 (MQ=60)
                                               CGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCG                                                >  SRR4302540.9268881/1‑94 (MQ=60)
                                               CGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCG                                                >  SRR4302540.3684387/1‑94 (MQ=60)
                                                GTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGT                                               <  SRR4302540.6045772/94‑1 (MQ=60)
                                                 TAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTT                                              >  SRR4302540.7773368/1‑94 (MQ=60)
                                                  AAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGAACATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTA                                             >  SRR4302540.6317041/1‑94 (MQ=60)
                                                   AAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAACGGTCGCGTTAT                                            >  SRR4302540.7582724/1‑94 (MQ=60)
                                                   AAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTAT                                            <  SRR4302540.9549048/94‑1 (MQ=60)
                                                   AAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTAT                                            >  SRR4302540.4098017/1‑94 (MQ=60)
                                                   AAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTAT                                            >  SRR4302540.5168982/1‑94 (MQ=60)
                                                   AAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTAT                                            <  SRR4302540.3891617/94‑1 (MQ=60)
                                                   AAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCCATATTGAAGAGCCAATTAAAGCTCGCGTTAT                                            <  SRR4302540.8911259/94‑1 (MQ=60)
                                                     GGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTG                                          >  SRR4302540.6888476/1‑94 (MQ=60)
                                                     GGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTG                                          <  SRR4302540.1713183/94‑1 (MQ=60)
                                                       CACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGAT                                        <  SRR4302540.914271/94‑1 (MQ=60)
                                                       CACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGAT                                        <  SRR4302540.9930284/94‑1 (MQ=60)
                                                       CACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGAT                                        <  SRR4302540.9466573/94‑1 (MQ=60)
                                                       CACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGAT                                        >  SRR4302540.6232710/1‑94 (MQ=60)
                                                       CACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGAT                                        >  SRR4302540.4710885/1‑94 (MQ=60)
                                                        ACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATG                                       >  SRR4302540.3901719/1‑94 (MQ=60)
                                                        ACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATG                                       >  SRR4302540.2975513/1‑94 (MQ=60)
                                                        ACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATG                                       >  SRR4302540.10840/1‑94 (MQ=60)
                                                        ACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATG                                       >  SRR4302540.5055503/1‑94 (MQ=60)
                                                        ACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGATCGCGTTATTGATG                                       >  SRR4302540.7276827/1‑94 (MQ=60)
                                                         CCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGA                                      <  SRR4302540.5925538/94‑1 (MQ=60)
                                                         CCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGA                                      >  SRR4302540.478356/1‑94 (MQ=60)
                                                          CGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAG                                     <  SRR4302540.8553827/94‑1 (MQ=60)
                                                          CGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATGGAAGAGCCAATTAAAGCTCGCGTTATTGATGAG                                     <  SRR4302540.1595612/94‑1 (MQ=60)
                                                            CAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTT                                   <  SRR4302540.10144032/94‑1 (MQ=60)
                                                            CAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTT                                   <  SRR4302540.611141/94‑1 (MQ=60)
                                                              ATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTAC                                 >  SRR4302540.4089039/1‑94 (MQ=60)
                                                              ATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTAC                                 <  SRR4302540.4708895/94‑1 (MQ=60)
                                                              ATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTAC                                 <  SRR4302540.731724/94‑1 (MQ=60)
                                                              ATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTAC                                 <  SRR4302540.7096846/94‑1 (MQ=60)
                                                               TTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACG                                <  SRR4302540.7527463/94‑1 (MQ=60)
                                                                  CGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCC                             <  SRR4302540.5194770/94‑1 (MQ=60)
                                                                  CGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGGAAAGTAGGCCGCTATATTGAAGACCCAATTAAAGCTCGCGTTATTGATGAGTTATGCCC                             >  SRR4302540.7877119/1‑94 (MQ=60)
                                                                   GCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTCCGCCCG                            >  SRR4302540.8904610/1‑94 (MQ=60)
                                                                   GCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCG                            >  SRR4302540.2012895/1‑94 (MQ=60)
                                                                   GCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCG                            <  SRR4302540.8237011/94‑1 (MQ=60)
                                                                    CTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGA                           >  SRR4302540.233878/1‑94 (MQ=60)
                                                                     TGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAA                          <  SRR4302540.4004059/94‑1 (MQ=60)
                                                                       GTGGAAGCCCATGACCATCTGGCGCTGGGTAAACTAGTCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAA                        <  SRR4302540.1418385/94‑1 (MQ=60)
                                                                       GTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAA                        <  SRR4302540.1418384/94‑1 (MQ=60)
                                                                       GTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAA                        <  SRR4302540.6464039/94‑1 (MQ=60)
                                                                        TGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAAC                       >  SRR4302540.7177473/1‑94 (MQ=60)
                                                                        GGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAAC                       <  SRR4302540.7838228/94‑1 (MQ=60)
                                                                             GCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTG                  >  SRR4302540.2792613/1‑94 (MQ=60)
                                                                              CCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGC                 <  SRR4302540.7126074/94‑1 (MQ=60)
                                                                              CCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGC                 <  SRR4302540.2634917/94‑1 (MQ=60)
                                                                               CCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCG                <  SRR4302540.10163018/94‑1 (MQ=60)
                                                                               CCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCG                <  SRR4302540.981719/94‑1 (MQ=60)
                                                                               CCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCG                <  SRR4302540.9162970/94‑1 (MQ=60)
                                                                               CCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCG                <  SRR4302540.4425004/94‑1 (MQ=60)
                                                                                 ATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCC              >  SRR4302540.1583414/1‑94 (MQ=60)
                                                                                 ATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCC              >  SRR4302540.2305737/1‑94 (MQ=60)
                                                                                 ATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCC              >  SRR4302540.9290378/1‑94 (MQ=60)
                                                                                  TGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCA             <  SRR4302540.8125073/94‑1 (MQ=60)
                                                                                  TGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCA             >  SRR4302540.4103407/1‑94 (MQ=60)
                                                                                    ACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAG           <  SRR4302540.3768530/94‑1 (MQ=60)
                                                                                     CCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGATCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGC          >  SRR4302540.5314780/1‑94 (MQ=60)
                                                                                      CATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCG         >  SRR4302540.973116/1‑94 (MQ=60)
                                                                                      CATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCG         >  SRR4302540.9875646/1‑94 (MQ=60)
                                                                                      CATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCG         <  SRR4302540.4010345/94‑1 (MQ=60)
                                                                                        TCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAA       <  SRR4302540.680648/94‑1 (MQ=60)
                                                                                         CTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAA      <  SRR4302540.9278818/94‑1 (MQ=60)
                                                                                         CTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAA      <  SRR4302540.2573369/94‑1 (MQ=60)
                                                                                          TGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAA     >  SRR4302540.4506921/1‑94 (MQ=60)
                                                                                           GCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAG    <  SRR4302540.3997359/94‑1 (MQ=60)
                                                                                            CCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGC   <  SRR4302540.4812411/94‑1 (MQ=60)
                                                                                             CGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCA  >  SRR4302540.4450541/1‑94 (MQ=60)
                                                                                             CGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCA  <  SRR4302540.2984146/94‑1 (MQ=60)
                                                                                             |                                                                                             
GTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCA  >  NC_000913/2711898‑2712084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑