Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,711,991 T→C L359P (CTG→CCG)  srmB → ATP‑dependent RNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,711,9910TC100.0% 643.3 / NA 198L359P (CTG→CCG) srmBATP‑dependent RNA helicase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (89/109);  total (89/109)

Only 100 of 207 total aligned reads displayed.

GCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAA  >  NC_000913/2711901‑2712080
                                                                                          |                                                                                         
gCGGTATTTATTTACACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCCCCGCAATTTCGCTGGTGGAAGCCCATGCCCATctgccgc                                                                                         <  2:2397822/93‑1 (MQ=255)
gCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATctgccgc                                                                                         >  1:1041879/1‑93 (MQ=255)
  gATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATctgccgctg                                                                                       <  2:3186859/93‑1 (MQ=255)
    tACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTggg                                                                                     >  1:1280092/1‑93 (MQ=255)
     aCTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTggga                                                                                    >  2:381743/1‑92 (MQ=255)
      cTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTa                                                                                   >  2:2484791/1‑93 (MQ=255)
       ttATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTaa                                                                                  >  2:1102649/1‑93 (MQ=255)
       ttATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTaa                                                                                  >  1:1019672/1‑93 (MQ=255)
         aTTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAg                                                                                <  1:3806950/93‑1 (MQ=255)
         aTTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAg                                                                                <  1:466839/93‑1 (MQ=255)
           ttGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTa                                                                              <  1:6172106/93‑1 (MQ=255)
           ttGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTa                                                                              >  2:6157200/1‑93 (MQ=255)
           ttGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTa                                                                              <  1:1522684/93‑1 (MQ=255)
           ttGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTa                                                                              <  2:5150484/93‑1 (MQ=255)
            tGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAg                                                                             >  2:5313636/1‑93 (MQ=255)
            tGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAg                                                                             >  2:2546657/1‑93 (MQ=255)
             gCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAgg                                                                            <  1:3056716/93‑1 (MQ=255)
               cccGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGcc                                                                          <  1:245855/92‑1 (MQ=255)
                ccGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCg                                                                         <  2:214612/93‑1 (MQ=255)
                 cGTATCGGACGTCCCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGc                                                                        <  2:1010690/93‑1 (MQ=255)
                 cGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGc                                                                        <  2:5779575/93‑1 (MQ=255)
                 cGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGc                                                                        >  1:442956/1‑93 (MQ=255)
                 cGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGc                                                                        <  1:220678/93‑1 (MQ=255)
                 cGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGACGc                                                                        >  2:701552/1‑93 (MQ=255)
                   tATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCta                                                                      >  2:1381244/1‑93 (MQ=255)
                     tCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCtata                                                                    <  1:3204037/93‑1 (MQ=255)
                     tCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATCACCATCTGCCGCTGGGTAAAGTAGGCCGCtata                                                                    >  2:571706/1‑93 (MQ=255)
                      cGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTatat                                                                   <  2:2612859/93‑1 (MQ=255)
                      cGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTatat                                                                   <  2:5966503/93‑1 (MQ=255)
                      cGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTatat                                                                   <  1:3633963/93‑1 (MQ=255)
                        gACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTg                                                                 <  2:3541872/93‑1 (MQ=255)
                         gcGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCAGCTGCCGCTGGGTAAAGTAGGCCGCTATATTGa                                                                <  1:3168752/92‑1 (MQ=255)
                         aCGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGa                                                                <  1:4861456/93‑1 (MQ=255)
                           gTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAag                                                              <  2:3781541/93‑1 (MQ=255)
                           gTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAag                                                              <  2:5424379/93‑1 (MQ=255)
                              ccGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGc                                                           >  1:5021345/1‑93 (MQ=255)
                                  gcgcgcCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAAt                                                       <  1:1146232/93‑1 (MQ=255)
                                   cgcgcCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAAtt                                                      <  2:652557/93‑1 (MQ=255)
                                      gcCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTaaa                                                   <  2:1307982/93‑1 (MQ=255)
                                      gcCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTaaa                                                   <  2:3835922/93‑1 (MQ=255)
                                        cGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGc                                                 >  2:1197202/1‑93 (MQ=255)
                                          gTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTc                                               >  2:4812171/1‑93 (MQ=255)
                                           tCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTcg                                              <  1:4638606/93‑1 (MQ=255)
                                             gTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTcgcg                                            <  1:814413/93‑1 (MQ=255)
                                             gTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTcgcg                                            <  1:4196443/93‑1 (MQ=255)
                                              tAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGt                                           >  1:247285/1‑93 (MQ=255)
                                               aaaGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGtt                                          >  1:4120385/1‑93 (MQ=255)
                                               aaaGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGATCGCGtt                                          >  2:2629281/1‑93 (MQ=255)
                                                aaGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTa                                         <  2:4324262/93‑1 (MQ=255)
                                                 aGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTAt                                        <  1:5818459/93‑1 (MQ=255)
                                                 aGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTAt                                        >  1:4088977/1‑93 (MQ=255)
                                                  ggCACCGCAGTTTCGCTGGTGCATGCCCATTACCATCTGCGGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTAtt                                       <  2:2829069/93‑1 (MQ=255)
                                                    cACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATtga                                     <  2:353534/93‑1 (MQ=255)
                                                    cACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATtga                                     <  2:2119776/93‑1 (MQ=255)
                                                      ccGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATtgatg                                   >  2:5585386/1‑93 (MQ=255)
                                                      ccGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATtgatg                                   <  2:4528233/93‑1 (MQ=255)
                                                       cGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATtgatga                                  >  1:5533127/1‑93 (MQ=255)
                                                        gCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAg                                 <  2:818171/93‑1 (MQ=255)
                                                          aaTTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGtt                               <  2:1421109/93‑1 (MQ=255)
                                                          aaTTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGtt                               <  1:5971811/93‑1 (MQ=255)
                                                          aaTTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGtt                               >  1:511503/1‑93 (MQ=255)
                                                          aaTTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGtt                               <  1:5373954/93‑1 (MQ=255)
                                                           aTTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTa                              <  1:1810589/93‑1 (MQ=255)
                                                            tttCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTAc                             <  2:4416983/93‑1 (MQ=255)
                                                            tttCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTAc                             <  2:1161489/93‑1 (MQ=255)
                                                             ttCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACg                            <  2:4268703/93‑1 (MQ=255)
                                                               cGCTGGTGGAAGCCCATGACCATCTTCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGcc                          <  1:2666273/93‑1 (MQ=255)
                                                               cGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGcc                          >  1:3532938/1‑93 (MQ=255)
                                                                gCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGATCGCGTTATTGATGAGTTACGccc                         >  1:4512777/1‑93 (MQ=255)
                                                                  tggtggAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGa                       >  2:201952/1‑93 (MQ=255)
                                                                  tggtggAAGCCCATGACCATCTGCCGCTGGGAAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCGCGCGTTATTGATGAGTTACGCCCGa                       >  1:4558140/1‑93 (MQ=255)
                                                                   ggtggAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGaa                      <  1:2816718/93‑1 (MQ=255)
                                                                   ggtggAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGaa                      >  1:6097950/1‑93 (MQ=255)
                                                                    gtggAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGaaa                     <  1:1316680/93‑1 (MQ=255)
                                                                     tggAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGaaaa                    <  2:4022983/93‑1 (MQ=255)
                                                                     tggAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGaaaa                    <  1:1489371/93‑1 (MQ=255)
                                                                      ggAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAAc                   <  2:2273694/93‑1 (MQ=255)
                                                                      ggAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAAc                   <  2:1533323/93‑1 (MQ=255)
                                                                       gAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAAcg                  >  1:4934033/1‑93 (MQ=255)
                                                                        aaGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAAcgc                 <  2:170509/93‑1 (MQ=255)
                                                                        aaGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAAcgc                 >  2:4496670/1‑93 (MQ=255)
                                                                          gCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGt               <  1:3496706/93‑1 (MQ=255)
                                                                             cATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGGGTgcg            >  2:2952479/1‑93 (MQ=255)
                                                                             cATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTgcg            >  1:3665956/1‑93 (MQ=255)
                                                                             cATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGATCGCGTTATTGATGAGTTACGCCCGAAAACGCGTgcg            >  1:3031481/1‑93 (MQ=255)
                                                                               tGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGcc          <  2:4388473/93‑1 (MQ=255)
                                                                               tGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGcc          >  1:4521486/1‑93 (MQ=255)
                                                                               tGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGcc          <  2:5626172/93‑1 (MQ=255)
                                                                                 aCCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCaa        <  1:258463/93‑1 (MQ=255)
                                                                                 aCCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCaa        <  1:335622/93‑1 (MQ=255)
                                                                                 aCCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCaa        >  1:5453078/1‑93 (MQ=255)
                                                                                  ccATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAg       >  1:1454051/1‑93 (MQ=255)
                                                                                  ccATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAg       >  1:881359/1‑93 (MQ=255)
                                                                                   cATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGc      <  1:3879156/93‑1 (MQ=255)
                                                                                   cATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGc      >  2:2700936/1‑93 (MQ=255)
                                                                                     tCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGa    >  1:3946357/1‑93 (MQ=255)
                                                                                     tCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGa    >  2:5036660/1‑93 (MQ=255)
                                                                                     tCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGa    >  2:2173646/1‑93 (MQ=255)
                                                                                     tCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGa    >  2:4127971/1‑93 (MQ=255)
                                                                                       tgccgctgGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGaaa  >  2:2704320/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAA  >  NC_000913/2711901‑2712080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCA  >  NC_000913/2711898‑2712084
                                                                                             |                                                                                             
GTGGCGATACTTATTTGCACCGTATCGTACTTACCGCGCGCTCCGTTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCC                                                                                               <  SRR4302533.3603487/94‑1 (MQ=60)
GTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCC                                                                                               <  SRR4302533.5763414/94‑1 (MQ=60)
GTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCC                                                                                               <  SRR4302533.1240608/94‑1 (MQ=60)
  GGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGC                                                                                             >  SRR4302533.1048005/1‑94 (MQ=60)
      ATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGG                                                                                         >  SRR4302533.1287787/1‑94 (MQ=60)
         CTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAA                                                                                      >  SRR4302533.1025665/1‑94 (MQ=60)
          TTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAA                                                                                     >  SRR4302533.359579/1‑94 (MQ=60)
          TTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGAAAA                                                                                     >  SRR4302533.4218173/1‑94 (MQ=60)
           TATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAG                                                                                    <  SRR4302533.4202603/94‑1 (MQ=60)
            TTTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGT                                                                                   <  SRR4302533.5290690/94‑1 (MQ=60)
            ATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGT                                                                                   <  SRR4302533.3830242/94‑1 (MQ=60)
            ATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGT                                                                                   <  SRR4302533.469627/94‑1 (MQ=60)
              TTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAG                                                                                 <  SRR4302533.6209196/94‑1 (MQ=60)
              TTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAG                                                                                 <  SRR4302533.3772023/94‑1 (MQ=60)
              TTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAG                                                                                 <  SRR4302533.1531848/94‑1 (MQ=60)
               TGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGG                                                                                <  SRR4302533.5913594/94‑1 (MQ=60)
                GCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGC                                                                               <  SRR4302533.3075466/94‑1 (MQ=60)
                 CACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCC                                                                              >  SRR4302533.5504536/1‑94 (MQ=60)
                  CCCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCG                                                                             <  SRR4302533.247378/94‑1 (MQ=60)
                  ACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCG                                                                             >  SRR4302533.1313943/1‑94 (MQ=60)
                   CCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGC                                                                            >  SRR4302533.445603/1‑94 (MQ=60)
                   CCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGC                                                                            >  SRR4302533.816225/1‑94 (MQ=60)
                    CGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCT                                                                           <  SRR4302533.222063/94‑1 (MQ=60)
                      TATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTAT                                                                         <  SRR4302533.3436115/94‑1 (MQ=60)
                       ATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATA                                                                        <  SRR4302533.4675091/94‑1 (MQ=60)
                       ATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATA                                                                        <  SRR4302533.1519630/94‑1 (MQ=60)
                        TCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATAT                                                                       <  SRR4302533.454175/94‑1 (MQ=60)
                        TCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATAT                                                                       <  SRR4302533.3223758/94‑1 (MQ=60)
                         CGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATT                                                                      <  SRR4302533.3656315/94‑1 (MQ=60)
                         CGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATT                                                                      <  SRR4302533.1478468/94‑1 (MQ=60)
                            GCGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCAGCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAA                                                                   <  SRR4302533.3188253/94‑1 (MQ=60)
                            ACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAA                                                                   <  SRR4302533.4890922/94‑1 (MQ=60)
                             CGTACCGCGCGCGCCGGTCGTAAAGGCACCGCACTTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAG                                                                  >  SRR4302533.2655317/1‑94 (MQ=60)
                              GTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGA                                                                 <  SRR4302533.1034142/94‑1 (MQ=60)
                              GTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGA                                                                 <  SRR4302533.2259613/94‑1 (MQ=60)
                               TACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAG                                                                <  SRR4302533.3053675/94‑1 (MQ=60)
                                ACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGC                                                               >  SRR4302533.5051755/1‑94 (MQ=60)
                                  CGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCA                                                             <  SRR4302533.39236/94‑1 (MQ=60)
                                    CGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAAT                                                           <  SRR4302533.3674493/94‑1 (MQ=60)
                                     GCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATT                                                          <  SRR4302533.1153072/94‑1 (MQ=60)
                                          CCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGC                                                     <  SRR4302533.869863/94‑1 (MQ=60)
                                          CCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGC                                                     >  SRR4302533.4124775/1‑94 (MQ=60)
                                           CGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCT                                                    >  SRR4302533.1603750/1‑94 (MQ=60)
                                             GTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCG                                                  <  SRR4302533.2892084/94‑1 (MQ=60)
                                              TCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGC                                                 <  SRR4302533.4666715/94‑1 (MQ=60)
                                                GTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGT                                               <  SRR4302533.819180/94‑1 (MQ=60)
                                                GTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGT                                               <  SRR4302533.4221848/94‑1 (MQ=60)
                                                GTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGT                                               >  SRR4302533.248813/1‑94 (MQ=60)
                                                 TAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTT                                              >  SRR4302533.4145405/1‑94 (MQ=60)
                                                 TAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTT                                              <  SRR4302533.334539/94‑1 (MQ=60)
                                                  AAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTA                                             >  SRR4302533.5446604/1‑94 (MQ=60)
                                                   AAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTAT                                            <  SRR4302533.2743627/94‑1 (MQ=60)
                                                   AAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTAT                                            >  SRR4302533.4113872/1‑94 (MQ=60)
                                                   AAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTAT                                            >  SRR4302533.4559037/1‑94 (MQ=60)
                                                    AGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATT                                           <  SRR4302533.5853607/94‑1 (MQ=60)
                                                         CCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGA                                      >  SRR4302533.5566534/1‑94 (MQ=60)
                                                         CCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGA                                      <  SRR4302533.4049436/94‑1 (MQ=60)
                                                          CGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAG                                     >  SRR4302533.6206096/1‑94 (MQ=60)
                                                           GCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGT                                    <  SRR4302533.5090425/94‑1 (MQ=60)
                                                           GCAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGT                                    <  SRR4302533.3546024/94‑1 (MQ=60)
                                                            CAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTT                                   >  SRR4302533.514543/1‑94 (MQ=60)
                                                            CAATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTT                                   >  SRR4302533.6104699/1‑94 (MQ=60)
                                                             AATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTA                                  <  SRR4302533.5406247/94‑1 (MQ=60)
                                                             AATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTA                                  <  SRR4302533.6007959/94‑1 (MQ=60)
                                                              ATTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTAC                                 <  SRR4302533.1821652/94‑1 (MQ=60)
                                                               TTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACG                                >  SRR4302533.2028620/1‑94 (MQ=60)
                                                               TTTCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACG                                <  SRR4302533.99338/94‑1 (MQ=60)
                                                                TTCGCTGGTGTAAGCCCATTCCCATCCGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGC                               <  SRR4302533.1270418/94‑1 (MQ=60)
                                                                 TCGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCC                              >  SRR4302533.3554708/1‑94 (MQ=60)
                                                                  CGCTGGTGGAAGCCCATGACCATCTTCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCC                             <  SRR4302533.2682633/94‑1 (MQ=60)
                                                                  CGCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGATCGCGTTATTGATGAGTTACGCCC                             >  SRR4302533.4540106/1‑94 (MQ=60)
                                                                   GCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGGCCG                            >  SRR4302533.4332756/1‑94 (MQ=60)
                                                                   GCTGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCG                            >  SRR4302533.2962347/1‑94 (MQ=60)
                                                                    CTGGTGGAAGCCCATGACCATCTGCCGCTGGGAAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCGCGCGTTATTGATGAGTTACGCCCGA                           >  SRR4302533.4585783/1‑94 (MQ=60)
                                                                     TGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAA                          <  SRR4302533.3899018/94‑1 (MQ=60)
                                                                     TGGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAA                          >  SRR4302533.6134780/1‑94 (MQ=60)
                                                                      GGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAA                         >  SRR4302533.4643668/1‑94 (MQ=60)
                                                                      GGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAA                         <  SRR4302533.2834058/94‑1 (MQ=60)
                                                                      GGTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAA                         >  SRR4302533.1061027/1‑94 (MQ=60)
                                                                       GTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAA                        >  SRR4302533.5798436/1‑94 (MQ=60)
                                                                       GTGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAA                        <  SRR4302533.1324578/94‑1 (MQ=60)
                                                                        TGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAATAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAAC                       <  SRR4302533.4466499/94‑1 (MQ=60)
                                                                        TGGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAAC                       <  SRR4302533.1498355/94‑1 (MQ=60)
                                                                         GGAAGCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACG                      >  SRR4302533.4963911/1‑94 (MQ=60)
                                                                             GCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTG                  <  SRR4302533.5838632/94‑1 (MQ=60)
                                                                             GCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTG                  >  SRR4302533.2546926/1‑94 (MQ=60)
                                                                             GCCCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTG                  <  SRR4302533.3518268/94‑1 (MQ=60)
                                                                               CCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCG                >  SRR4302533.3688491/1‑94 (MQ=60)
                                                                               CCATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGATCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCG                >  SRR4302533.3050031/1‑94 (MQ=60)
                                                                                CATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGTCCGAAAACGCGTGCGC               >  SRR4302533.3195552/1‑94 (MQ=60)
                                                                                CATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGC               <  SRR4302533.143636/94‑1 (MQ=60)
                                                                                CATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGC               >  SRR4302533.2475503/1‑94 (MQ=60)
                                                                                 ATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCC              >  SRR4302533.4760412/1‑94 (MQ=60)
                                                                                 ATGACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCC              >  SRR4302533.4548864/1‑94 (MQ=60)
                                                                                   GACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAA            >  SRR4302533.3121079/1‑94 (MQ=60)
                                                                                   GACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAA            >  SRR4302533.5485923/1‑94 (MQ=60)
                                                                                   GACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAA            >  SRR4302533.4954311/1‑94 (MQ=60)
                                                                                    ACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAG           >  SRR4302533.1462821/1‑94 (MQ=60)
                                                                                    ACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAG           <  SRR4302533.260036/94‑1 (MQ=60)
                                                                                    ACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAG           <  SRR4302533.337673/94‑1 (MQ=60)
                                                                                    ACCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAG           >  SRR4302533.886535/1‑94 (MQ=60)
                                                                                     CCATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGC          <  SRR4302533.498782/94‑1 (MQ=60)
                                                                                      CATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCG         <  SRR4302533.3902827/94‑1 (MQ=60)
                                                                                       ATCTGCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGA        >  SRR4302533.3970425/1‑94 (MQ=60)
                                                                                           GCCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAG    <  SRR4302533.3264124/94‑1 (MQ=60)
                                                                                            CCGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGC   >  SRR4302533.1045768/1‑94 (MQ=60)
                                                                                             CGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCA  >  SRR4302533.4618818/1‑94 (MQ=60)
                                                                                             |                                                                                             
GTGGCGATACTTATTTGCACCGTATCGGACGTACCGCGCGCGCCGGTCGTAAAGGCACCGCAATTTCGCTGGTGGAAGCCCATGACCATCTGCTGCTGGGTAAAGTAGGCCGCTATATTGAAGAGCCAATTAAAGCTCGCGTTATTGATGAGTTACGCCCGAAAACGCGTGCGCCAAGCGAAAAGCA  >  NC_000913/2711898‑2712084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑