Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,825,102	T→C	F230L (TTC→CTC)	srlE →	PTS system glucitol/sorbitol‑specific enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,825,102	0	T	C	100.0%	800.8 / NA	247	F230L (TTC→CTC)	srlE	PTS system glucitol/sorbitol‑specific enzyme IIBC component
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (118/129); total (118/129)

Only 100 of 265 total aligned reads displayed.

ATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCTTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTA  >  NC_000913/2825012‑2825191

aTTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTttcctc                                                                                         <  1:5319939/93‑1 (MQ=255)

aTTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTttcctc                                                                                         <  1:5568032/93‑1 (MQ=255)

 ttGCCCACGGTCTTGCTCCGATGGCGAGCGATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCg                                                                                        <  2:2875949/93‑1 (MQ=255)

     ccACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGccc                                                                                    <  1:9752155/93‑1 (MQ=255)

      cACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCa                                                                                   >  2:2337013/1‑93 (MQ=255)

      cACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCa                                                                                   <  2:2219968/93‑1 (MQ=255)

       ccGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAg                                                                                  <  1:4612296/92‑1 (MQ=255)

       aCGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAg                                                                                  <  2:8879470/93‑1 (MQ=255)

        cGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTTCTTTCACCTTTCCTCGGCCCAgg                                                                                 >  2:7208472/1‑93 (MQ=255)

        cGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAgg                                                                                 <  2:3680024/93‑1 (MQ=255)

         ggTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgc                                                                                >  2:947031/1‑93 (MQ=255)

         ggTCTGGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgc                                                                                <  1:4094408/93‑1 (MQ=255)

            cTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCa                                                                             <  2:7464680/93‑1 (MQ=255)

            cTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCa                                                                             <  1:9870309/93‑1 (MQ=255)

             ttGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAg                                                                            >  2:1538561/1‑93 (MQ=255)

             ttGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAg                                                                            >  2:2435458/1‑93 (MQ=255)

                cTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTa                                                                         <  2:6857286/93‑1 (MQ=255)

                cTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTa                                                                         <  2:3880973/93‑1 (MQ=255)

                 cccGCGGGCCAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTAt                                                                        <  1:290343/92‑1 (MQ=255)

                  ccGCTGGCGAGGCATCCGCTCGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATc                                                                       <  1:3343533/93‑1 (MQ=255)

                    gCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGc                                                                     <  1:9103937/93‑1 (MQ=255)

                    gCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGc                                                                     <  2:4302613/93‑1 (MQ=255)

                     cTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGGGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcc                                                                    >  1:1909899/1‑92 (MQ=255)

                      tGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcac                                                                   >  1:8491222/1‑93 (MQ=255)

                      tGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcac                                                                   <  1:8287189/93‑1 (MQ=255)

                       ggCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcaca                                                                  >  2:8110275/1‑93 (MQ=255)

                       ggCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcaca                                                                  <  2:1044224/93‑1 (MQ=255)

                            gCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTa                                                             >  1:5802307/1‑93 (MQ=255)

                             ccATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTAt                                                            <  2:7885459/93‑1 (MQ=255)

                             ccATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTAt                                                            <  2:682570/93‑1 (MQ=255)

                             ccATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTAt                                                            <  2:8606020/93‑1 (MQ=255)

                             ccATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTAt                                                            <  2:1249492/93‑1 (MQ=255)

                              cATCCGCTTGGTCTGGTCATGATGGCGCTCAACTGCTCCTTCCCAGTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATc                                                           <  2:5398641/93‑1 (MQ=255)

                              cATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATc                                                           <  2:6390463/93‑1 (MQ=255)

                              cATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATc                                                           <  2:846418/93‑1 (MQ=255)

                                tCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCgg                                                         >  2:8306000/1‑93 (MQ=255)

                                 ccACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCTCAGGCGCAGTTATCGCACAGGTTATCGGc                                                        <  2:6217667/93‑1 (MQ=255)

                                 ccACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGc                                                        >  1:9302605/1‑93 (MQ=255)

                                 ccACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGc                                                        >  2:382168/1‑93 (MQ=255)

                                  cACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCg                                                       >  1:9260537/1‑93 (MQ=255)

                                   aCTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGt                                                      >  2:1982910/1‑93 (MQ=255)

                                   aCTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGt                                                      >  2:4727486/1‑93 (MQ=255)

                                   aCTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGt                                                      <  1:1382616/93‑1 (MQ=255)

                                   aCTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGt                                                      >  1:7837601/1‑93 (MQ=255)

                                     tGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTat                                                    <  2:7957121/93‑1 (MQ=255)

                                       ggTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattg                                                  >  2:5967182/1‑93 (MQ=255)

                                        gTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattga                                                 <  2:3838880/93‑1 (MQ=255)

                                         tCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgat                                                <  1:8699013/93‑1 (MQ=255)

                                          cTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgatt                                               <  1:7687649/93‑1 (MQ=255)

                                           tGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTatttattg                                              >  2:1901497/1‑93 (MQ=255)

                                           tGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTGTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgattg                                              <  2:1686775/93‑1 (MQ=255)

                                             gTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGc                                            <  2:9252199/93‑1 (MQ=255)

                                              tCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCg                                           <  1:5013515/93‑1 (MQ=255)

                                                 tGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGc                                        >  2:1273405/1‑93 (MQ=255)

                                                 tGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGc                                        <  2:9898596/93‑1 (MQ=255)

                                                  gCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCa                                       <  2:8723639/93‑1 (MQ=255)

                                                  gCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCa                                       <  1:2387810/93‑1 (MQ=255)

                                                   cTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAg                                      <  1:739229/93‑1 (MQ=255)

                                                   cTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAg                                      <  1:7033520/93‑1 (MQ=255)

                                                     ggCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAt                                    <  1:6349354/93‑1 (MQ=255)

                                                     ggCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAt                                    >  1:1978885/1‑93 (MQ=255)

                                                     ggCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAt                                    <  1:664695/93‑1 (MQ=255)

                                                       cgcTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTg                                  >  2:2958764/1‑93 (MQ=255)

                                                        gcTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTgg                                 <  1:3907266/93‑1 (MQ=255)

                                                         cTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGt                                <  1:8221894/93‑1 (MQ=255)

                                                         cTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGt                                >  1:4056416/1‑93 (MQ=255)

                                                           cATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtct                              >  1:2891131/1‑93 (MQ=255)

                                                            ctCTGCTCCTTCCCACTGCTTTCACCTTTCCGCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtctc                             <  1:5655103/92‑1 (MQ=255)

                                                            aTCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtctc                             <  1:4645298/93‑1 (MQ=255)

                                                             tCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCg                            <  1:1698400/93‑1 (MQ=255)

                                                             tCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCg                            >  1:4342803/1‑93 (MQ=255)

                                                             tCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCg                            >  1:7767453/1‑93 (MQ=255)

                                                                gctccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCa                         >  2:4998261/3‑93 (MQ=255)

                                                                 ctccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCaa                        <  2:2740671/92‑1 (MQ=255)

                                                                  tccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAat                       >  2:6169328/1‑93 (MQ=255)

                                                                  tccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAat                       <  1:8065899/93‑1 (MQ=255)

                                                                  tccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCCCAGTTATCGCCCAGGTTATCGGCGCATTGATTTGCGTACAGATTCAGCTCCGCAat                       >  2:7362657/1‑93 (MQ=255)

                                                                   ccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGGGTATTAATTGGCGTGCAGATTGGTCTCGGCAata                      >  1:743679/1‑93 (MQ=255)

                                                                     ttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATAtt                    >  1:372014/1‑93 (MQ=255)

                                                                     ttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATAtt                    >  2:1196150/1‑93 (MQ=255)

                                                                      tccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTc                   <  1:646558/93‑1 (MQ=255)

                                                                      tccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTc                   <  1:767469/93‑1 (MQ=255)

                                                                       cccACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTcc                  >  1:8837999/1‑93 (MQ=255)

                                                                        ccACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTccg                 <  1:2061064/93‑1 (MQ=255)

                                                                        ccACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTccg                 <  1:3047651/93‑1 (MQ=255)

                                                                         cACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTccgc                <  2:7583143/93‑1 (MQ=255)

                                                                         cACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTccgc                <  1:2504427/93‑1 (MQ=255)

                                                                           cTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTccgccg              >  2:7061477/1‑93 (MQ=255)

                                                                           cTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTccgccg              >  2:6932294/1‑93 (MQ=255)

                                                                             gCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCa            >  1:552695/1‑93 (MQ=255)

                                                                              cTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCAt           >  1:2829825/1‑93 (MQ=255)

                                                                                ttCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCt         <  2:5665113/93‑1 (MQ=255)

                                                                                ttCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCt         >  1:5180024/1‑93 (MQ=255)

                                                                                 tCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTg        >  2:904304/1‑93 (MQ=255)

                                                                                  cACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTgg       >  1:8120079/1‑93 (MQ=255)

                                                                                  cACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTgg       >  2:5132632/1‑93 (MQ=255)

                                                                                   cccTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGc      <  2:330888/92‑1 (MQ=255)

                                                                                     cTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCtt    <  2:4836308/93‑1 (MQ=255)

                                                                                      tttCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCttt   >  1:5971374/1‑93 (MQ=255)

                                                                                       ttcctcGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTa  >  2:4575726/1‑93 (MQ=255)

ATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCTTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTA  >  NC_000913/2825012‑2825191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCTTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGG  >  NC_000913/2825009‑2825195

TGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCCTCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCC                                                                                               >  SRR4302540.8044871/1‑94 (MQ=60)

TGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCC                                                                                               <  SRR4302540.4957391/94‑1 (MQ=60)

 GGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCT                                                                                              <  SRR4302540.3529054/94‑1 (MQ=60)

 GGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCT                                                                                              <  SRR4302540.5355488/94‑1 (MQ=60)

  GATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGGTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTC                                                                                             >  SRR4302540.4902086/1‑94 (MQ=60)

  GATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTC                                                                                             <  SRR4302540.1068373/94‑1 (MQ=60)

  GATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTC                                                                                             <  SRR4302540.8506300/94‑1 (MQ=60)

  GATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTC                                                                                             <  SRR4302540.8506301/94‑1 (MQ=60)

   ATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCG                                                                                            <  SRR4302540.5629344/94‑1 (MQ=60)

   ATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCG                                                                                            <  SRR4302540.5378198/94‑1 (MQ=60)

   ATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCG                                                                                            <  SRR4302540.39160/94‑1 (MQ=60)

      GCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCC                                                                                         >  SRR4302540.8098222/1‑94 (MQ=60)

       CCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCC                                                                                        >  SRR4302540.3197208/1‑94 (MQ=60)

        CCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCA                                                                                       <  SRR4302540.9866583/94‑1 (MQ=60)

          CCGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGG                                                                                     <  SRR4302540.4661791/94‑1 (MQ=60)

          ACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGG                                                                                     >  SRR4302540.1008633/1‑94 (MQ=60)

            GGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCG                                                                                   >  SRR4302540.8896938/1‑94 (MQ=60)

            GGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCG                                                                                   <  SRR4302540.4691109/94‑1 (MQ=60)

            GGTCTGGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCG                                                                                   <  SRR4302540.4138583/94‑1 (MQ=60)

              tctttgtgcgcttgccAGCCATGCACTGGGTCTTGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCA                                                                                 <  SRR4302540.2516309/78‑1 (MQ=60)

               CTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAG                                                                                >  SRR4302540.377245/1‑94 (MQ=60)

               CTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAG                                                                                <  SRR4302540.9985586/94‑1 (MQ=60)

               CTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAG                                                                                >  SRR4302540.1873163/1‑94 (MQ=60)

                TTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGT                                                                               <  SRR4302540.4383213/94‑1 (MQ=60)

                 TGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTT                                                                              <  SRR4302540.2353845/94‑1 (MQ=60)

                 TGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTT                                                                              >  SRR4302540.5808556/1‑94 (MQ=60)

                   GTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTAT                                                                            <  SRR4302540.9400180/94‑1 (MQ=60)

                    cccgcgggccAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATC                                                                           <  SRR4302540.293214/84‑1 (MQ=60)

                    TCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATC                                                                           <  SRR4302540.293213/94‑1 (MQ=60)

                     CCGCTGGCGAGGCATCCGCTCGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCG                                                                          <  SRR4302540.3380220/94‑1 (MQ=60)

                     CCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCG                                                                          >  SRR4302540.3197499/1‑94 (MQ=60)

                      CGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGC                                                                         >  SRR4302540.7540665/1‑94 (MQ=60)

                      CGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGC                                                                         >  SRR4302540.7540667/1‑94 (MQ=60)

                       GCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGGGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCC                                                                        >  SRR4302540.1930766/1‑94 (MQ=60)

                       GCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCA                                                                        <  SRR4302540.9213334/94‑1 (MQ=60)

                        CTGGGGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCAC                                                                       <  SRR4302540.1958625/94‑1 (MQ=60)

                        CTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCAC                                                                       >  SRR4302540.8789396/1‑94 (MQ=60)

                        CTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCAC                                                                       <  SRR4302540.4128797/94‑1 (MQ=60)

                        CTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCAC                                                                       >  SRR4302540.8589881/1‑94 (MQ=60)

                         TGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACA                                                                      <  SRR4302540.8383433/94‑1 (MQ=60)

                          GGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAG                                                                     <  SRR4302540.6159492/94‑1 (MQ=60)

                              AGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTA                                                                 >  SRR4302540.5866418/1‑94 (MQ=60)

                              AGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTA                                                                 >  SRR4302540.5866417/1‑94 (MQ=60)

                               GCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTAT                                                                <  SRR4302540.7070173/94‑1 (MQ=60)

                                CCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATC                                                               <  SRR4302540.8166183/94‑1 (MQ=60)

                                 CATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCG                                                              >  SRR4302540.7310220/1‑94 (MQ=60)

                                 CATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCG                                                              >  SRR4302540.7310219/1‑94 (MQ=60)

                                 CATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCG                                                              >  SRR4302540.648196/1‑94 (MQ=60)

                                   TCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGC                                                            >  SRR4302540.9413718/1‑94 (MQ=60)

                                   TCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGC                                                            >  SRR4302540.5622157/1‑94 (MQ=60)

                                    CCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCG                                                           >  SRR4302540.9371321/1‑94 (MQ=60)

                                     CACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGT                                                          <  SRR4302540.204032/94‑1 (MQ=60)

                                     CACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGT                                                          >  SRR4302540.7929170/1‑94 (MQ=60)

                                     CACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGT                                                          >  SRR4302540.1869781/1‑94 (MQ=60)

                                      ACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTA                                                         <  SRR4302540.1397374/94‑1 (MQ=60)

                                       CTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTAT                                                        >  SRR4302540.6599558/1‑94 (MQ=60)

                                         GGGTCTGGTCATGCTGGCGCTCATCTGCCCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTG                                                      >  SRR4302540.8768122/1‑94 (MQ=60)

                                          GGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGA                                                     >  SRR4302540.9970214/1‑94 (MQ=60)

                                          GGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGA                                                     >  SRR4302540.6524955/1‑94 (MQ=60)

                                           GTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGAT                                                    >  SRR4302540.4427157/1‑94 (MQ=60)

                                            TCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATT                                                   <  SRR4302540.8799994/94‑1 (MQ=60)

                                            GCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATT                                                   <  SRR4302540.2689286/94‑1 (MQ=60)

                                             CTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTG                                                  <  SRR4302540.6178822/94‑1 (MQ=60)

                                             CTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTG                                                  <  SRR4302540.781612/94‑1 (MQ=60)

                                             CTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTG                                                  <  SRR4302540.9747565/94‑1 (MQ=60)

                                             CTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTG                                                  <  SRR4302540.7774914/94‑1 (MQ=60)

                                               TGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGCTATCGCACAGGTTATCGGCGTATTGATTGGC                                                <  SRR4302540.9878719/94‑1 (MQ=60)

                                                GTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAAGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCG                                               <  SRR4302540.6829958/94‑1 (MQ=60)

                                                 TCATGCTGGCGCTTATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGT                                              <  SRR4302540.8338052/94‑1 (MQ=60)

                                                 TCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGT                                              <  SRR4302540.5067797/94‑1 (MQ=60)

                                                   ATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGC                                            >  SRR4302540.854144/1‑94 (MQ=60)

                                                   ATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGA                                            >  SRR4302540.4034082/1‑94 (MQ=60)

                                                     GCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAG                                          <  SRR4302540.2413546/94‑1 (MQ=60)

                                                      CTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGA                                         <  SRR4302540.7111695/94‑1 (MQ=60)

                                                      CTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGA                                         <  SRR4302540.747121/94‑1 (MQ=60)

                                                       TGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAT                                        >  SRR4302540.2000356/1‑94 (MQ=60)

                                                        GGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATT                                       <  SRR4302540.671824/94‑1 (MQ=60)

                                                        GGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATT                                       <  SRR4302540.6420061/94‑1 (MQ=60)

                                                        GGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATT                                       <  SRR4302540.8305937/94‑1 (MQ=60)

                                                         GCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTG                                      <  SRR4302540.4219785/94‑1 (MQ=60)

                                                          CGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGG                                     >  SRR4302540.2073/1‑94 (MQ=60)

                                                           GCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGT                                    <  SRR4302540.3949621/94‑1 (MQ=60)

                                                           GCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGT                                    >  SRR4302540.4100150/1‑94 (MQ=60)

                                                            CTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTC                                   <  SRR4302540.8317466/94‑1 (MQ=60)

                                                            CTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTC                                   >  SRR4302540.5706023/1‑94 (MQ=60)

                                                             TCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCT                                  >  SRR4302540.2113092/1‑94 (MQ=60)

                                                             TCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCT                                  >  SRR4302540.349095/1‑94 (MQ=60)

                                                             TCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCT                                  >  SRR4302540.2922638/1‑94 (MQ=60)

                                                             TCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCT                                  >  SRR4302540.2922637/1‑94 (MQ=60)

                                                              catcgtctcattccgcctgtTTTCACCGTTCCTCGGCCCAGGCGCAGTTATCGCAAAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTC                                 <  SRR4302540.7946563/74‑1 (MQ=60)

                                                              CATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTC                                 <  SRR4302540.7946562/94‑1 (MQ=60)

                                                              CATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTC                                 >  SRR4302540.2717165/1‑94 (MQ=60)

                                                               CTCTGCTCCTTCCCACTGCTTTCACCTTTCCGCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCG                                <  SRR4302540.5717371/94‑1 (MQ=60)

                                                               ATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCG                                >  SRR4302540.291710/1‑94 (MQ=60)

                                                               ATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCG                                >  SRR4302540.7858261/1‑94 (MQ=60)

                                                               ATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCG                                >  SRR4302540.4390075/1‑94 (MQ=60)

                                                               ATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCG                                <  SRR4302540.5710896/94‑1 (MQ=60)

                                                               ATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCG                                <  SRR4302540.4695021/94‑1 (MQ=60)

                                                               ATCTGCTCCTTCCCACTGCTTGCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCG                                >  SRR4302540.7858262/1‑94 (MQ=60)

                                                                cattctccttccctctgcttctcCCTTTCCTCGGCCGAGGCGCAGTTATCGCACATGGTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGG                               <  SRR4302540.7544293/71‑1 (MQ=60)

                                                                TCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGG                               <  SRR4302540.1716799/94‑1 (MQ=60)

                                                                   GCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAA                            >  SRR4302540.1426661/1‑94 (MQ=60)

                                                                     TCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGGGTATTAATTGGCGTGCAGATTGGTCTCGGCAATA                          >  SRR4302540.751606/1‑94 (MQ=60)

                                                                     TCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATA                          >  SRR4302540.751605/1‑94 (MQ=60)

                                                                     TCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATA                          <  SRR4302540.6032033/94‑1 (MQ=60)

                                                                     TCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATA                          >  SRR4302540.5049191/1‑94 (MQ=60)

                                                                     TCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATA                          <  SRR4302540.8159503/94‑1 (MQ=60)

                                                                      CCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATAT                         <  SRR4302540.508639/94‑1 (MQ=60)

                                                                       CTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATT                        >  SRR4302540.5488266/1‑94 (MQ=60)

                                                                       CTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATT                        >  SRR4302540.375858/1‑94 (MQ=60)

                                                                         TCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCC                      >  SRR4302540.8940222/1‑94 (MQ=60)

                                                                         TCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCC                      <  SRR4302540.9236320/94‑1 (MQ=60)

                                                                         TCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCC                      <  SRR4302540.653445/94‑1 (MQ=60)

                                                                         TCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCC                      <  SRR4302540.775645/94‑1 (MQ=60)

                                                                         TCCCACTGCTGTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCC                      <  SRR4302540.8302425/94‑1 (MQ=60)

                                                                          CCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCG                     <  SRR4302540.3036668/94‑1 (MQ=60)

                                                                          CCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCG                     >  SRR4302540.5099050/1‑94 (MQ=60)

                                                                           CCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGC                    <  SRR4302540.2083244/94‑1 (MQ=60)

                                                                           CCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGC                    <  SRR4302540.3081090/94‑1 (MQ=60)

                                                                            CACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCC                   <  SRR4302540.2531456/94‑1 (MQ=60)

                                                                             CCTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCG                  <  SRR4302540.7756788/94‑1 (MQ=60)

                                                                             ACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCG                  >  SRR4302540.7766305/1‑94 (MQ=60)

                                                                             ACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCG                  >  SRR4302540.7766306/1‑94 (MQ=60)

                                                                              CTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGC                 <  SRR4302540.8347568/94‑1 (MQ=60)

                                                                              CTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGC                 >  SRR4302540.4038105/1‑94 (MQ=60)

                                                                               TGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCA                >  SRR4302540.558722/1‑94 (MQ=60)

                                                                               TGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCA                <  SRR4302540.7865969/94‑1 (MQ=60)

                                                                                GCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGGTTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCAT               <  SRR4302540.5554896/94‑1 (MQ=60)

                                                                                GCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCAT               >  SRR4302540.2860434/1‑94 (MQ=60)

                                                                                  TTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCT             >  SRR4302540.5236401/1‑94 (MQ=60)

                                                                                   TTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTG            <  SRR4302540.9570807/94‑1 (MQ=60)

                                                                                   TTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTG            <  SRR4302540.4474093/94‑1 (MQ=60)

                                                                                    TCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGG           >  SRR4302540.8214315/1‑94 (MQ=60)

                                                                                    TCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGG           <  SRR4302540.2509023/94‑1 (MQ=60)

                                                                                     CACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTAGTGATTGGCGGGCAGATTGGCCGCGCACATATTCGGCCGCATCTGGC          >  SRR4302540.9251515/1‑94 (MQ=60)

                                                                                       CCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTT        <  SRR4302540.5269930/94‑1 (MQ=60)

                                                                                        CTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTT       <  SRR4302540.8966641/94‑1 (MQ=60)

                                                                                        CTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTT       <  SRR4302540.8966636/94‑1 (MQ=60)

                                                                                        CTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTT       >  SRR4302540.6037466/1‑94 (MQ=60)

                                                                                          TTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTAC     <  SRR4302540.2974316/94‑1 (MQ=60)

                                                                                           TCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACC    <  SRR4302540.5416361/94‑1 (MQ=60)

                                                                                           TCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACC    >  SRR4302540.2141791/1‑94 (MQ=60)

                                                                                            CCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCG   <  SRR4302540.5566830/94‑1 (MQ=60)

                                                                                             CTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGG  <  SRR4302540.9178513/94‑1 (MQ=60)

                                                                                             CTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGG  <  SRR4302540.3672086/94‑1 (MQ=60)

TGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCTTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCGG  >  NC_000913/2825009‑2825195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`