Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,825,102	T→C	F230L (TTC→CTC)	srlE →	PTS system glucitol/sorbitol‑specific enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,825,102	0	T	C	100.0%	790.2 / NA	244	F230L (TTC→CTC)	srlE	PTS system glucitol/sorbitol‑specific enzyme IIBC component
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (97/147); total (97/147)

Only 100 of 261 total aligned reads displayed.

ATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCTTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTA  >  NC_000913/2825012‑2825191

aTTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTttcctc                                                                                         <  1:1677883/93‑1 (MQ=255)

 ttGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCg                                                                                        <  1:4921892/93‑1 (MQ=255)

    cccACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGcc                                                                                     <  1:5862495/93‑1 (MQ=255)

     ccACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGccc                                                                                    <  1:384562/93‑1 (MQ=255)

      cACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCa                                                                                   >  2:5729770/1‑93 (MQ=255)

        cGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAgg                                                                                 <  1:1057142/93‑1 (MQ=255)

        cGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAgg                                                                                 <  2:6194934/93‑1 (MQ=255)

         ggTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgc                                                                                <  2:5612583/93‑1 (MQ=255)

         ggTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgc                                                                                >  2:5645554/1‑93 (MQ=255)

          gTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgcg                                                                               >  1:4503780/1‑93 (MQ=255)

          gTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTAATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgcg                                                                               >  2:5025573/1‑93 (MQ=255)

           tCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgcgc                                                                              <  1:2316004/93‑1 (MQ=255)

              tGCTCCGATGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGt                                                                           <  2:2268567/93‑1 (MQ=255)

               gCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGtt                                                                          >  1:2013061/1‑93 (MQ=255)

               gCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGtt                                                                          >  1:2351337/1‑93 (MQ=255)

               gCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGtt                                                                          <  1:1559839/93‑1 (MQ=255)

                cTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTa                                                                         <  1:3672210/93‑1 (MQ=255)

                  ccGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATc                                                                       <  1:5764973/93‑1 (MQ=255)

                  ccGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATc                                                                       <  1:5042645/93‑1 (MQ=255)

                  ccGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATc                                                                       <  1:130793/93‑1 (MQ=255)

                     cTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGTAGTTATCGca                                                                    <  2:3202320/93‑1 (MQ=255)

                      tGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCTCACTGCTTTCACCCTTCCTCGGCCCATGCGCAGTTATCCcac                                                                   >  2:2996403/1‑93 (MQ=255)

                       ggCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcaca                                                                  <  2:4911321/93‑1 (MQ=255)

                       ggCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcaca                                                                  >  1:261856/1‑93 (MQ=255)

                       ggCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcaca                                                                  <  2:607874/93‑1 (MQ=255)

                        gCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAg                                                                 <  1:3737082/93‑1 (MQ=255)

                          gAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGt                                                               <  2:2357932/93‑1 (MQ=255)

                          gAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGt                                                               <  1:5364935/93‑1 (MQ=255)

                          gAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGt                                                               <  2:1294641/93‑1 (MQ=255)

                           aGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGtt                                                              <  1:3416253/93‑1 (MQ=255)

                            gCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTa                                                             <  2:4376719/93‑1 (MQ=255)

                            gCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTa                                                             <  1:609451/93‑1 (MQ=255)

                            gCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTa                                                             <  2:1834524/93‑1 (MQ=255)

                             ccATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTAt                                                            <  2:6292225/93‑1 (MQ=255)

                              cATCCACTGGGTCTGGTCATGCTGGCTCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATc                                                           <  2:5820651/93‑1 (MQ=255)

                              cATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATc                                                           >  2:3551739/1‑93 (MQ=255)

                              cATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATc                                                           >  2:4827337/1‑93 (MQ=255)

                              cATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATc                                                           <  1:1217610/93‑1 (MQ=255)

                                  cACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCATAGGTTATCGGCg                                                       >  1:3150206/1‑93 (MQ=255)

                                  cACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCg                                                       <  2:1155775/93‑1 (MQ=255)

                                  cACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCg                                                       >  1:899416/1‑93 (MQ=255)

                                   aCTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGt                                                      >  1:472763/1‑93 (MQ=255)

                                   aCTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGt                                                      <  2:1033011/93‑1 (MQ=255)

                                      gggTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTatt                                                   <  2:4880024/93‑1 (MQ=255)

                                           tGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgattg                                              <  1:5564659/93‑1 (MQ=255)

                                           tGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgattg                                              >  2:2133424/1‑93 (MQ=255)

                                              tCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCg                                           >  1:5478172/1‑93 (MQ=255)

                                                aTGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTg                                         >  2:2531434/1‑93 (MQ=255)

                                                  gCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCa                                       >  1:6280582/1‑93 (MQ=255)

                                                  gCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCa                                       <  2:6120972/93‑1 (MQ=255)

                                                  gCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCa                                       <  1:1096719/93‑1 (MQ=255)

                                                  gCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCa                                       >  1:4298878/1‑93 (MQ=255)

                                                     ggCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAt                                    <  1:1848177/93‑1 (MQ=255)

                                                     ggCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAt                                    <  2:5858551/93‑1 (MQ=255)

                                                      gcgcTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAtt                                   <  2:5477932/93‑1 (MQ=255)

                                                      gcgcTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCGCGGCCCAGGCGCAGTTATCGCTCAGGTTATCGGCGTATTGATTGGCGTGCAGAtt                                   <  2:449392/93‑1 (MQ=255)

                                                        gcTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTgg                                 <  2:550745/93‑1 (MQ=255)

                                                         cTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGt                                <  2:839251/93‑1 (MQ=255)

                                                         cTCATCTGCTCCTTCACACTGCTTTCACCTTTCCTGGGCCCAGGCGCAGTTATCGCACACGTTATCGGCGTATTGATTGGAGTGAAGATTggc                                >  2:686021/1‑92 (MQ=255)

                                                            aTCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtctc                             >  1:1167807/1‑93 (MQ=255)

                                                             tCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCg                            <  1:2649381/93‑1 (MQ=255)

                                                             tCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCg                            >  2:376496/1‑93 (MQ=255)

                                                                 ctccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCaa                        <  2:3006299/92‑1 (MQ=255)

                                                                  tccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAat                       >  1:4351788/1‑93 (MQ=255)

                                                                  tccttccCACTGCTTGCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAat                       <  2:5800019/93‑1 (MQ=255)

                                                                   ccttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAata                      <  2:4372190/93‑1 (MQ=255)

                                                                    cttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAatat                     >  1:3999481/1‑93 (MQ=255)

                                                                     ttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATAtt                    >  1:5209798/1‑93 (MQ=255)

                                                                     ttccCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATAtt                    >  1:4886371/1‑93 (MQ=255)

                                                                       cccACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTcc                  <  2:697717/93‑1 (MQ=255)

                                                                       cccACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTcc                  <  1:4228419/93‑1 (MQ=255)

                                                                       cccACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTcc                  <  1:2175977/93‑1 (MQ=255)

                                                                        ccACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTccg                 <  1:3545142/93‑1 (MQ=255)

                                                                         cACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTccgc                >  1:2974713/1‑93 (MQ=255)

                                                                         cACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTccgc                >  2:3721170/1‑93 (MQ=255)

                                                                            tGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCcgccgc             <  1:3191363/93‑1 (MQ=255)

                                                                            tGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTACCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCcgccgc             <  2:5106129/93‑1 (MQ=255)

                                                                             gCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCa            <  2:1535925/93‑1 (MQ=255)

                                                                              cTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCAt           >  2:275246/1‑93 (MQ=255)

                                                                              cTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCAt           >  1:5421262/1‑93 (MQ=255)

                                                                               tttCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATc          <  1:5964564/93‑1 (MQ=255)

                                                                               tttCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATc          >  1:4653630/1‑93 (MQ=255)

                                                                               tttCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATc          <  1:2793514/93‑1 (MQ=255)

                                                                                ttCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCt         <  1:1021511/93‑1 (MQ=255)

                                                                                ttCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCt         <  2:3502210/93‑1 (MQ=255)

                                                                                 tCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATTGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTg        >  2:1649892/1‑93 (MQ=255)

                                                                                 tCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTg        <  1:3948347/93‑1 (MQ=255)

                                                                                 tCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTg        <  1:2332516/93‑1 (MQ=255)

                                                                                 tCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTg        <  1:6259476/93‑1 (MQ=255)

                                                                                   aCCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGc      >  2:3305969/1‑93 (MQ=255)

                                                                                   aCCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGc      >  2:6010413/1‑93 (MQ=255)

                                                                                    ccTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCt     <  2:1715803/93‑1 (MQ=255)

                                                                                     cTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCtt    <  2:5187842/93‑1 (MQ=255)

                                                                                     cTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCtt    >  2:2461117/1‑93 (MQ=255)

                                                                                     cTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCtt    >  1:2748224/1‑93 (MQ=255)

                                                                                      tttCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCttt   <  2:4519249/93‑1 (MQ=255)

                                                                                      tttCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCttt   >  1:2472134/1‑93 (MQ=255)

                                                                                      tttCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCttt   >  1:711309/1‑93 (MQ=255)

                                                                                       ttcctcGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTa  >  2:3934861/1‑93 (MQ=255)

                                                                                       ttcctcGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTa  <  2:3619326/93‑1 (MQ=255)

ATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCTTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTA  >  NC_000913/2825012‑2825191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCTTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCG  >  NC_000913/2825009‑2825194

TGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCC                                                                                              >  SRR4302533.2282019/1‑94 (MQ=60)

TGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCC                                                                                              >  SRR4302533.3761881/1‑94 (MQ=60)

 GGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCT                                                                                             <  SRR4302533.3287686/94‑1 (MQ=60)

 GGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCT                                                                                             <  SRR4302533.340035/94‑1 (MQ=60)

 GGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCT                                                                                             <  SRR4302533.1270670/94‑1 (MQ=60)

  GATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTC                                                                                            >  SRR4302533.3370052/1‑94 (MQ=60)

   ATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCG                                                                                           <  SRR4302533.1180488/94‑1 (MQ=60)

   ATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCG                                                                                           <  SRR4302533.4875605/94‑1 (MQ=60)

   ATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCG                                                                                           <  SRR4302533.1688043/94‑1 (MQ=60)

    TTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGG                                                                                          >  SRR4302533.2153392/1‑94 (MQ=60)

    TTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGG                                                                                          <  SRR4302533.4951705/94‑1 (MQ=60)

     TGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGC                                                                                         <  SRR4302533.264871/94‑1 (MQ=60)

       CCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCC                                                                                       >  SRR4302533.38706/1‑94 (MQ=60)

       CCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCC                                                                                       <  SRR4302533.5897941/94‑1 (MQ=60)

        CCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCA                                                                                      <  SRR4302533.386883/94‑1 (MQ=60)

          ACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGG                                                                                    >  SRR4302533.5461962/1‑94 (MQ=60)

          ACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGG                                                                                    <  SRR4302533.5568177/94‑1 (MQ=60)

          ACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGC                                                                                    >  SRR4302533.1089343/1‑94 (MQ=60)

           CGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGC                                                                                   <  SRR4302533.1063358/94‑1 (MQ=60)

           CGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGC                                                                                   >  SRR4302533.4534554/1‑94 (MQ=60)

            GGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCG                                                                                  <  SRR4302533.4005681/94‑1 (MQ=60)

            GGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCG                                                                                  >  SRR4302533.4531063/1‑94 (MQ=60)

             GTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGC                                                                                 >  SRR4302533.2483269/1‑94 (MQ=60)

              TCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCA                                                                                <  SRR4302533.2330345/94‑1 (MQ=60)

               CTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAG                                                                               <  SRR4302533.1252083/94‑1 (MQ=60)

               CTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAG                                                                               <  SRR4302533.4985895/94‑1 (MQ=60)

                 TGCTCCGCTGTCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTT                                                                             <  SRR4302533.5658893/94‑1 (MQ=60)

                 TGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTT                                                                             <  SRR4302533.550604/94‑1 (MQ=60)

                 TGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTT                                                                             >  SRR4302533.2025460/1‑94 (MQ=60)

                 TGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTT                                                                             >  SRR4302533.2365883/1‑94 (MQ=60)

                  GCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTA                                                                            <  SRR4302533.1569235/94‑1 (MQ=60)

                  GCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTA                                                                            <  SRR4302533.352443/94‑1 (MQ=60)

                   CTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTAT                                                                           <  SRR4302533.3694778/94‑1 (MQ=60)

                    TCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATC                                                                          <  SRR4302533.3759154/94‑1 (MQ=60)

                    TCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATC                                                                          >  SRR4302533.483608/1‑94 (MQ=60)

                    GCCGCTGGCGAGACATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATC                                                                          <  SRR4302533.3905937/94‑1 (MQ=60)

                     CCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCG                                                                         <  SRR4302533.2803319/94‑1 (MQ=60)

                     CCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCG                                                                         <  SRR4302533.4313328/94‑1 (MQ=60)

                     CCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCG                                                                         <  SRR4302533.5073163/94‑1 (MQ=60)

                     CCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCG                                                                         <  SRR4302533.131612/94‑1 (MQ=60)

                     CCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCG                                                                         <  SRR4302533.5799763/94‑1 (MQ=60)

                       GCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCA                                                                       >  SRR4302533.5820584/1‑94 (MQ=60)

                       GCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTACCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCA                                                                       <  SRR4302533.4474866/94‑1 (MQ=60)

                         TGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACA                                                                     >  SRR4302533.263443/1‑94 (MQ=60)

                          GGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAG                                                                    <  SRR4302533.6300892/94‑1 (MQ=60)

                          GGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAG                                                                    <  SRR4302533.6094308/94‑1 (MQ=60)

                           GCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGG                                                                   <  SRR4302533.1097811/94‑1 (MQ=60)

                           GCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGG                                                                   <  SRR4302533.3760006/94‑1 (MQ=60)

                           GCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCAAGGCGCAGTTATCGCACAGG                                                                   <  SRR4302533.3188404/94‑1 (MQ=60)

                             GAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTT                                                                 <  SRR4302533.5397163/94‑1 (MQ=60)

                              AGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTA                                                                <  SRR4302533.3437359/94‑1 (MQ=60)

                               GCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTAT                                                               <  SRR4302533.613035/94‑1 (MQ=60)

                                 CATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCG                                                             <  SRR4302533.1224920/94‑1 (MQ=60)

                                 CATCCACTGGGGCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCG                                                             <  SRR4302533.1681245/94‑1 (MQ=60)

                                  ATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGG                                                            >  SRR4302533.3627519/1‑94 (MQ=60)

                                   TCCACTGGGTCTGGTCATGCTGTCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGC                                                           <  SRR4302533.2749700/94‑1 (MQ=60)

                                   TCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGC                                                           <  SRR4302533.2749702/94‑1 (MQ=60)

                                    CCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCATAGGTTATCGGCG                                                          >  SRR4302533.3169577/1‑94 (MQ=60)

                                    CCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCG                                                          >  SRR4302533.904695/1‑94 (MQ=60)

                                     CACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGT                                                         >  SRR4302533.475590/1‑94 (MQ=60)

                                       CTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTAT                                                       <  SRR4302533.2185247/94‑1 (MQ=60)

                                         GGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTG                                                     <  SRR4302533.4002062/94‑1 (MQ=60)

                                           GTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGAT                                                   <  SRR4302533.5711994/94‑1 (MQ=60)

                                             CTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTG                                                 <  SRR4302533.4937183/94‑1 (MQ=60)

                                             CTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTG                                                 >  SRR4302533.234449/1‑94 (MQ=60)

                                              TGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGG                                                <  SRR4302533.133078/94‑1 (MQ=60)

                                              TGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGG                                                <  SRR4302533.54171/94‑1 (MQ=60)

                                              TGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGG                                                <  SRR4302533.5598294/94‑1 (MQ=60)

                                               GGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGC                                               >  SRR4302533.5226402/1‑94 (MQ=60)

                                                GTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCG                                              <  SRR4302533.2513435/94‑1 (MQ=60)

                                                GTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCG                                              >  SRR4302533.4438034/1‑94 (MQ=60)

                                                GTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCG                                              <  SRR4302533.6306194/94‑1 (MQ=60)

                                                GTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCG                                              >  SRR4302533.5511196/1‑94 (MQ=60)

                                                 TCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGT                                             <  SRR4302533.2463522/94‑1 (MQ=60)

                                                    TGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCA                                          >  SRR4302533.4324733/1‑94 (MQ=60)

                                                    TGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCA                                          <  SRR4302533.6113661/94‑1 (MQ=60)

                                                    TGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCA                                          >  SRR4302533.6318070/1‑94 (MQ=60)

                                                     GCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAG                                         <  SRR4302533.1103214/94‑1 (MQ=60)

                                                       TGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAT                                       >  SRR4302533.1665604/1‑94 (MQ=60)

                                                        GGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATT                                      <  SRR4302533.1859449/94‑1 (MQ=60)

                                                         GCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACCGGTTATCGGCGTATTGATTGGCGTGCAGATTG                                     >  SRR4302533.2924870/1‑94 (MQ=60)

                                                          CGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGG                                    <  SRR4302533.971482/94‑1 (MQ=60)

                                                          CGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGG                                    >  SRR4302533.3462099/1‑94 (MQ=60)

                                                           GCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGT                                   >  SRR4302533.5988512/1‑94 (MQ=60)

                                                            CTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTC                                  <  SRR4302533.5453151/94‑1 (MQ=60)

                                                            CTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGATATCGGCGTATTGATTGGCGTGCAGATTGGTC                                  >  SRR4302533.1190930/1‑94 (MQ=60)

                                                              CATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTC                                >  SRR4302533.1174813/1‑94 (MQ=60)

                                                              CATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTC                                >  SRR4302533.1926257/1‑94 (MQ=60)

                                                               ATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCG                               >  SRR4302533.5145054/1‑94 (MQ=60)

                                                               ATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCG                               <  SRR4302533.2587542/94‑1 (MQ=60)

                                                                TCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGG                              <  SRR4302533.2665656/94‑1 (MQ=60)

                                                                 CTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGC                             >  SRR4302533.1309028/1‑94 (MQ=60)

                                                                   GCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGAGTGCAGATTGGTCTCGGCAA                           >  SRR4302533.1052043/1‑94 (MQ=60)

                                                                    CTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAAT                          >  SRR4302533.4378048/1‑94 (MQ=60)

                                                                     TCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATA                         <  SRR4302533.4138958/94‑1 (MQ=60)

                                                                     TCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATA                         >  SRR4302533.4294466/1‑94 (MQ=60)

                                                                     CCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATA                         <  SRR4302533.4728008/94‑1 (MQ=60)

                                                                      CCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATAT                        >  SRR4302533.3289512/1‑94 (MQ=60)

                                                                      CCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATAT                        >  SRR4302533.686370/1‑94 (MQ=60)

                                                                      CCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATAT                        >  SRR4302533.4023833/1‑94 (MQ=60)

                                                                       CTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATT                       >  SRR4302533.395889/1‑94 (MQ=60)

                                                                       CTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATT                       >  SRR4302533.4915977/1‑94 (MQ=60)

                                                                       CTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATT                       >  SRR4302533.2341684/1‑94 (MQ=60)

                                                                       CTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATT                       <  SRR4302533.4988933/94‑1 (MQ=60)

                                                                       CTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATT                       >  SRR4302533.5241178/1‑94 (MQ=60)

                                                                        TTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTC                      >  SRR4302533.185375/1‑94 (MQ=60)

                                                                          CCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCG                    <  SRR4302533.2189389/94‑1 (MQ=60)

                                                                          CCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCG                    <  SRR4302533.230082/94‑1 (MQ=60)

                                                                          CCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCG                    <  SRR4302533.4253984/94‑1 (MQ=60)

                                                                          CCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCG                    <  SRR4302533.3100466/94‑1 (MQ=60)

                                                                           CCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGC                   >  SRR4302533.2992913/1‑94 (MQ=60)

                                                                           CCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGC                   <  SRR4302533.3566992/94‑1 (MQ=60)

                                                                            CACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCC                  <  SRR4302533.1944840/94‑1 (MQ=60)

                                                                             CCTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCG                 <  SRR4302533.94984/94‑1 (MQ=60)

                                                                               TGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCA               <  SRR4302533.3210994/94‑1 (MQ=60)

                                                                               TGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCA               >  SRR4302533.3155486/1‑94 (MQ=60)

                                                                                GCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCAT              >  SRR4302533.5453884/1‑94 (MQ=60)

                                                                                 CTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATC             >  SRR4302533.4545536/1‑94 (MQ=60)

                                                                                 CTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATC             >  SRR4302533.4681828/1‑94 (MQ=60)

                                                                                 CTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATC             <  SRR4302533.5950260/94‑1 (MQ=60)

                                                                                  TTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCT            <  SRR4302533.6000656/94‑1 (MQ=60)

                                                                                  TTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCT            <  SRR4302533.2810697/94‑1 (MQ=60)

                                                                                   TTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTG           <  SRR4302533.1027513/94‑1 (MQ=60)

                                                                                    TCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGG          <  SRR4302533.2346957/94‑1 (MQ=60)

                                                                                    TCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGG          <  SRR4302533.6296902/94‑1 (MQ=60)

                                                                                    TCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGG          <  SRR4302533.3972424/94‑1 (MQ=60)

                                                                                     CACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGC         >  SRR4302533.375309/1‑94 (MQ=60)

                                                                                     CACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGC         <  SRR4302533.752587/94‑1 (MQ=60)

                                                                                      ACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCT        <  SRR4302533.778722/94‑1 (MQ=60)

                                                                                      ACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCT        >  SRR4302533.6227599/1‑94 (MQ=60)

                                                                                       CCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTT       >  SRR4302533.2765072/1‑94 (MQ=60)

                                                                                       CCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTT       >  SRR4302533.2361647/1‑94 (MQ=60)

                                                                                       CCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTT       <  SRR4302533.2927871/94‑1 (MQ=60)

                                                                                        CTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTT      <  SRR4302533.1164547/94‑1 (MQ=60)

                                                                                        CTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTT      >  SRR4302533.4331789/1‑94 (MQ=60)

                                                                                        CTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTT      >  SRR4302533.715509/1‑94 (MQ=60)

                                                                                        CTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTT      >  SRR4302533.2487378/1‑94 (MQ=60)

                                                                                         TTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTA     >  SRR4302533.534682/1‑94 (MQ=60)

                                                                                          TTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTAC    >  SRR4302533.5615980/1‑94 (MQ=60)

                                                                                           TCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACC   <  SRR4302533.570638/94‑1 (MQ=60)

                                                                                           TCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACC   >  SRR4302533.815962/1‑94 (MQ=60)

                                                                                            CCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCG  <  SRR4302533.3203579/94‑1 (MQ=60)

                                                                                            CCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCG  <  SRR4302533.1958803/94‑1 (MQ=60)

                                                                                            CCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCG  <  SRR4302533.3046858/94‑1 (MQ=60)

TGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCTTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCGGCAATATTCCGCCGCATCTGGCTTTACCG  >  NC_000913/2825009‑2825194

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`