Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,977,219 T→C H59H (CAT→CAC)  lysR → LysR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,977,2190TC99.5% 1277.2 / ‑3.4 394H59H (CAT→CAC) lysRLysR
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base C (157/235);  total (157/237)
Fisher's exact test for biased strand distribution p-value = 5.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.14e-01

Only 100 of 409 total aligned reads displayed.

CACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGC  >  NC_000913/2977129‑2977307
                                                                                          |                                                                                        
cacCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACAccc                                                                                        <  2:10037386/93‑1 (MQ=255)
cacCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACAccc                                                                                        <  2:7147382/93‑1 (MQ=255)
   cTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTAc                                                                                     <  1:9443517/93‑1 (MQ=255)
   cTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTAc                                                                                     <  1:428096/93‑1 (MQ=255)
   cTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTAc                                                                                     <  2:2840857/93‑1 (MQ=255)
    tCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTAcc                                                                                    <  2:9371375/93‑1 (MQ=255)
    tCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTAcc                                                                                    <  2:2075920/93‑1 (MQ=255)
    tCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTAcc                                                                                    <  2:2603074/93‑1 (MQ=255)
     cacaGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCg                                                                                   <  2:8684728/93‑1 (MQ=255)
        aGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGc                                                                                >  2:1909057/1‑93 (MQ=255)
         gCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCa                                                                               <  1:6816702/93‑1 (MQ=255)
           cAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTTCCGTGCAAg                                                                             <  2:8557045/93‑1 (MQ=255)
            aaCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAgg                                                                            <  1:6507915/93‑1 (MQ=255)
            aaCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAgg                                                                            <  1:876949/93‑1 (MQ=255)
            aaCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAgg                                                                            >  1:5039005/1‑93 (MQ=255)
            aaCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAgg                                                                            <  1:2022529/93‑1 (MQ=255)
             aCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGa                                                                           >  2:3482154/1‑93 (MQ=255)
             aCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGa                                                                           <  2:8299785/93‑1 (MQ=255)
             aCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGa                                                                           >  2:1280844/1‑93 (MQ=255)
             aCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGa                                                                           <  2:9519450/93‑1 (MQ=255)
              ccGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGTCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGAc                                                                          <  1:2900402/93‑1 (MQ=255)
               cGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACt                                                                         <  1:778323/93‑1 (MQ=255)
                gTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTg                                                                        >  2:3149460/1‑93 (MQ=255)
                gTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTg                                                                        <  2:1576417/93‑1 (MQ=255)
                gTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTg                                                                        >  2:987160/1‑93 (MQ=255)
                 tCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGc                                                                       <  1:9194772/93‑1 (MQ=255)
                  cAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCg                                                                      >  1:2108143/1‑93 (MQ=255)
                   aGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGt                                                                     <  2:3229233/93‑1 (MQ=255)
                   aGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGt                                                                     >  1:1828508/1‑93 (MQ=255)
                    gCCGCGATCGTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTc                                                                    <  2:1104208/93‑1 (MQ=255)
                    gCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTc                                                                    <  1:7292963/93‑1 (MQ=255)
                    gCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTc                                                                    <  1:2639785/93‑1 (MQ=255)
                    gCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTc                                                                    >  2:1690392/1‑93 (MQ=255)
                       gcgAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGt                                                                 >  2:7848930/1‑93 (MQ=255)
                        cgAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGtt                                                                <  1:4494273/93‑1 (MQ=255)
                         gAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACGCCCTACCGTGCAAGGACTGCGTCTGttt                                                               <  2:8805367/93‑1 (MQ=255)
                         gAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGttt                                                               <  2:5735823/93‑1 (MQ=255)
                            cTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTgaa                                                            >  2:137033/1‑93 (MQ=255)
                             ttGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTgaag                                                           >  2:6103565/1‑93 (MQ=255)
                             ttGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTgaag                                                           >  1:368078/1‑93 (MQ=255)
                              tGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTgaaga                                                          <  2:7356309/93‑1 (MQ=255)
                               gcgcgcTTTGAGAAGGTTATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTgaagaa                                                         <  2:4365966/93‑1 (MQ=255)
                                 gcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGt                                                       <  2:3380197/93‑1 (MQ=255)
                                    cTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCa                                                    >  2:639877/1‑93 (MQ=255)
                                     tttGAGAAGGTGATCGGGCTGAAATTGTTTGATCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCaa                                                   >  1:9392409/1‑93 (MQ=255)
                                     tttGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCaa                                                   <  1:1408041/93‑1 (MQ=255)
                                      ttGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAAc                                                  <  2:5584819/93‑1 (MQ=255)
                                      ttGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAAc                                                  <  1:6271736/93‑1 (MQ=255)
                                      ttGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAAc                                                  <  1:6976744/93‑1 (MQ=255)
                                        gagaAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGa                                                <  2:5452014/93‑1 (MQ=255)
                                        gagaAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGa                                                <  2:6017837/93‑1 (MQ=255)
                                         agaAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCACCGAt                                               >  1:8459713/1‑93 (MQ=255)
                                         agaAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGAt                                               >  1:8535905/1‑93 (MQ=255)
                                         agaAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGAt                                               >  2:309318/1‑93 (MQ=255)
                                          gagggTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGAGCAAGGACTGCGGCTGTTTGAAGAAGTGCAACGATc                                              >  2:4542295/4‑93 (MQ=255)
                                          gaAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATc                                              <  2:6288918/93‑1 (MQ=255)
                                          gaAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATc                                              <  1:4326335/93‑1 (MQ=255)
                                          gaAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATc                                              <  1:6655959/93‑1 (MQ=255)
                                          gaAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATc                                              >  2:9136016/1‑93 (MQ=255)
                                           aaGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATcc                                             <  2:1471709/93‑1 (MQ=255)
                                           aaGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATcc                                             <  1:4441476/93‑1 (MQ=255)
                                           aaGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATcc                                             <  1:3430994/93‑1 (MQ=255)
                                            aGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCt                                            >  1:9767960/1‑93 (MQ=255)
                                             ggTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTg                                           <  1:8912265/93‑1 (MQ=255)
                                               tGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGCCTGACTCTGTTTGAAGAAGTGCAACGATCCTgga                                         >  1:769598/1‑92 (MQ=255)
                                                    gggCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGa                                    >  2:8187282/1‑93 (MQ=255)
                                                     ggCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGAc                                   <  1:4733490/93‑1 (MQ=255)
                                                        tGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTgg                                <  1:2336146/93‑1 (MQ=255)
                                                        tGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTgg                                >  1:10043851/1‑93 (MQ=255)
                                                         gAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGa                               >  2:5150223/1‑93 (MQ=255)
                                                          aaaTTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGAt                              >  2:4654612/1‑93 (MQ=255)
                                                           aaTTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATc                             <  2:3720415/93‑1 (MQ=255)
                                                           aaTTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATc                             <  1:1747469/93‑1 (MQ=255)
                                                           aaTTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATc                             <  2:5264605/93‑1 (MQ=255)
                                                           aaNTGTTTGNGCGCGTACGTGNNCGNTTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATc                             <  1:605527/93‑1 (MQ=255)
                                                            aTTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCg                            <  2:5001018/93‑1 (MQ=255)
                                                              tGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCa                          >  2:4210796/1‑93 (MQ=255)
                                                              tGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCa                          >  1:1323042/1‑93 (MQ=255)
                                                               gTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAt                         <  1:8159364/93‑1 (MQ=255)
                                                                 ttGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTg                       <  2:2934542/93‑1 (MQ=255)
                                                                   gAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTc                     <  2:6716174/93‑1 (MQ=255)
                                                                    aGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCa                    <  2:8427677/93‑1 (MQ=255)
                                                                    aGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCa                    >  1:5453614/1‑93 (MQ=255)
                                                                    aGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCa                    >  2:7698305/1‑93 (MQ=255)
                                                                       gcgTACGTGTGCGATTACACCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGTACTGGATCGCATTGTCAgcg                 <  2:6837273/93‑1 (MQ=255)
                                                                       gcgTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAgcg                 >  2:2322712/1‑93 (MQ=255)
                                                                          tACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgccg              >  2:4115920/1‑93 (MQ=255)
                                                                          tACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgccg              >  1:5208640/1‑93 (MQ=255)
                                                                             gTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAg           <  1:1137345/93‑1 (MQ=255)
                                                                               gggCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGaa         >  1:302912/1‑93 (MQ=255)
                                                                                ggCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGaaa        >  1:95611/1‑93 (MQ=255)
                                                                                ggCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGaaa        <  1:4823685/93‑1 (MQ=255)
                                                                                 gCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAg       <  1:3335446/93‑1 (MQ=255)
                                                                                 gCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAg       >  2:8898540/1‑93 (MQ=255)
                                                                                   gATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTc     <  1:5885215/93‑1 (MQ=255)
                                                                                   gATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTc     <  1:3874074/93‑1 (MQ=255)
                                                                                   gATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTc     <  1:3984533/93‑1 (MQ=255)
                                                                                    aTTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCt    >  1:8229326/1‑93 (MQ=255)
                                                                                     ttACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTg   >  1:7872019/1‑93 (MQ=255)
                                                                                     ttACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTg   <  1:8352801/93‑1 (MQ=255)
                                                                                          |                                                                                        
CACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGC  >  NC_000913/2977129‑2977307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 216 total aligned reads displayed.
ACACACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAG  >  NC_000913/2977126‑2977312
                                                                                             |                                                                                             
ACACACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACAC                                                                                               <  SRR4302540.9278945/94‑1 (MQ=60)
ACACACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACAC                                                                                               <  SRR4302540.4661926/94‑1 (MQ=60)
 CACACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACC                                                                                              <  SRR4302540.3734132/94‑1 (MQ=60)
  ccgCCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCC                                                                                             <  SRR4302540.2878724/91‑1 (MQ=60)
  ccacctccgAGCCACCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCC                                                                                             <  SRR4302540.9917849/85‑1 (MQ=60)
  ACACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCC                                                                                             <  SRR4302540.2667419/94‑1 (MQ=60)
  ACACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCC                                                                                             <  SRR4302540.7165868/94‑1 (MQ=60)
  ACACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCC                                                                                             >  SRR4302540.9531672/1‑94 (MQ=60)
   CACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCT                                                                                            <  SRR4302540.7886811/94‑1 (MQ=60)
   CACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCT                                                                                            <  SRR4302540.649610/94‑1 (MQ=60)
   CACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCT                                                                                            >  SRR4302540.9561518/1‑94 (MQ=60)
   CACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCT                                                                                            >  SRR4302540.9561519/1‑94 (MQ=60)
     CCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTAC                                                                                          <  SRR4302540.4344840/94‑1 (MQ=60)
      CTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACC                                                                                         <  SRR4302540.432598/94‑1 (MQ=60)
      CTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACC                                                                                         <  SRR4302540.7838319/94‑1 (MQ=60)
      CTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACC                                                                                         >  SRR4302540.1216646/1‑94 (MQ=60)
         ACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTG                                                                                      >  SRR4302540.138162/1‑94 (MQ=60)
         ACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTG                                                                                      >  SRR4302540.8364323/1‑94 (MQ=60)
         ACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTG                                                                                      >  SRR4302540.8517790/1‑94 (MQ=60)
            GCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAG                                                                                   >  SRR4302540.8426469/1‑94 (MQ=60)
            GCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAA                                                                                   <  SRR4302540.6892153/94‑1 (MQ=60)
             CCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAG                                                                                  >  SRR4302540.458964/1‑94 (MQ=60)
             CCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAG                                                                                  >  SRR4302540.9840134/1‑94 (MQ=60)
              CAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGG                                                                                 >  SRR4302540.5093513/1‑94 (MQ=60)
              CAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGG                                                                                 >  SRR4302540.138171/1‑94 (MQ=60)
              CAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGGGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGG                                                                                 >  SRR4302540.166193/1‑94 (MQ=60)
               AACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGA                                                                                >  SRR4302540.7092858/1‑94 (MQ=60)
               AACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGA                                                                                <  SRR4302540.886260/94‑1 (MQ=60)
               AACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGA                                                                                <  SRR4302540.2044389/94‑1 (MQ=60)
               AACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGA                                                                                >  SRR4302540.7286521/1‑94 (MQ=60)
               AACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGA                                                                                <  SRR4302540.6580234/94‑1 (MQ=60)
                ACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGAC                                                                               <  SRR4302540.4533040/94‑1 (MQ=60)
                ACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGAC                                                                               <  SRR4302540.4533041/94‑1 (MQ=60)
                 CCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGTCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACT                                                                              <  SRR4302540.2932052/94‑1 (MQ=60)
                 CCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACT                                                                              <  SRR4302540.2932050/94‑1 (MQ=60)
                 CCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACT                                                                              <  SRR4302540.353702/94‑1 (MQ=60)
                 CCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACT                                                                              <  SRR4302540.9552486/94‑1 (MQ=60)
                  CGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTG                                                                             <  SRR4302540.786621/94‑1 (MQ=60)
                  CGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTG                                                                             <  SRR4302540.6165785/94‑1 (MQ=60)
                  CGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTG                                                                             >  SRR4302540.1268523/1‑94 (MQ=60)
                   GTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGG                                                                            >  SRR4302540.1889483/1‑94 (MQ=60)
                   GTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGC                                                                            <  SRR4302540.7053914/94‑1 (MQ=60)
                    TCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCG                                                                           >  SRR4302540.2130768/1‑94 (MQ=60)
                    TCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCG                                                                           <  SRR4302540.9305061/94‑1 (MQ=60)
                     CAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGT                                                                          >  SRR4302540.1848509/1‑94 (MQ=60)
                      AGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTC                                                                         >  SRR4302540.3624807/1‑94 (MQ=60)
                       GCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCT                                                                        <  SRR4302540.2668387/94‑1 (MQ=60)
                       GCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCT                                                                        <  SRR4302540.7374349/94‑1 (MQ=60)
                       GCCGCGAACTTGCGCGCTTTGAGAAGGTGATCAGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTATCGTGCAAGGACTGCGTCT                                                                        <  SRR4302540.4358254/94‑1 (MQ=60)
                        GCGCGAACTTGCGGGCTTTGAGAAGGTGGTCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTG                                                                       <  SRR4302540.2117208/94‑1 (MQ=60)
                        CCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGCGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTG                                                                       >  SRR4302540.1262213/1‑94 (MQ=60)
                         CGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGT                                                                      <  SRR4302540.9147209/94‑1 (MQ=60)
                          GCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTT                                                                     <  SRR4302540.4468203/94‑1 (MQ=60)
                          GCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTT                                                                     <  SRR4302540.5167047/94‑1 (MQ=60)
                           CGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTT                                                                    >  SRR4302540.5785548/1‑94 (MQ=60)
                           CGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTT                                                                    <  SRR4302540.4543000/94‑1 (MQ=60)
                            GAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTG                                                                   <  SRR4302540.5385904/94‑1 (MQ=60)
                             AACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGA                                                                  >  SRR4302540.6681919/1‑94 (MQ=60)
                             AACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGA                                                                  >  SRR4302540.6681917/1‑94 (MQ=60)
                              ACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAA                                                                 <  SRR4302540.9540345/94‑1 (MQ=60)
                              ACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAA                                                                 >  SRR4302540.319429/1‑94 (MQ=60)
                              ACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAA                                                                 >  SRR4302540.7604847/1‑94 (MQ=60)
                              ACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAA                                                                 <  SRR4302540.2544013/94‑1 (MQ=60)
                               CTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTaagc                                                                >  SRR4302540.4337278/1‑90 (MQ=60)
                               CTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAG                                                                >  SRR4302540.371870/1‑94 (MQ=60)
                               CTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAG                                                                <  SRR4302540.1804894/94‑1 (MQ=60)
                               CTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAG                                                                <  SRR4302540.8836729/94‑1 (MQ=60)
                                TTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGCAGA                                                               >  SRR4302540.3937113/1‑94 (MQ=60)
                                TTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGA                                                               >  SRR4302540.475934/1‑94 (MQ=60)
                                 TGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAA                                                              <  SRR4302540.252731/94‑1 (MQ=60)
                                 TGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAA                                                              <  SRR4302540.138464/94‑1 (MQ=60)
                                   CGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGT                                                            <  SRR4302540.9336863/94‑1 (MQ=60)
                                    GCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTG                                                           >  SRR4302540.6705737/1‑94 (MQ=60)
                                      GCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCA                                                         >  SRR4302540.3394226/1‑94 (MQ=60)
                                      GCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCA                                                         <  SRR4302540.8256663/94‑1 (MQ=60)
                                      GCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCA                                                         <  SRR4302540.817842/94‑1 (MQ=60)
                                       CTTTGAGAAGGTGATCGGGCTGAAATTGTTTGATCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAA                                                        >  SRR4302540.9504179/1‑94 (MQ=60)
                                        atgGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACCCCCTGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAAC                                                       <  SRR4302540.8270019/91‑1 (MQ=60)
                                        TTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAAC                                                       <  SRR4302540.9943237/94‑1 (MQ=60)
                                        TTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAAC                                                       >  SRR4302540.8567811/1‑94 (MQ=60)
                                        TTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAAC                                                       <  SRR4302540.8270020/94‑1 (MQ=60)
                                        TTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAAC                                                       <  SRR4302540.2985581/94‑1 (MQ=60)
                                        TGTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAAC                                                       <  SRR4302540.9806680/94‑1 (MQ=60)
                                         TTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTGCGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACG                                                      <  SRR4302540.5884880/94‑1 (MQ=60)
                                         TTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACG                                                      <  SRR4302540.7054262/94‑1 (MQ=60)
                                         TTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACG                                                      >  SRR4302540.1052058/1‑94 (MQ=60)
                                         TTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACG                                                      <  SRR4302540.6341518/94‑1 (MQ=60)
                                         TTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACG                                                      <  SRR4302540.9645460/94‑1 (MQ=60)
                                           GAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGAT                                                    >  SRR4302540.8635089/1‑94 (MQ=60)
                                           GAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGAT                                                    <  SRR4302540.1897940/94‑1 (MQ=60)
                                            AGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATC                                                   >  SRR4302540.4981740/1‑94 (MQ=60)
                                            AGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATC                                                   >  SRR4302540.1613782/1‑94 (MQ=60)
                                             GAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCC                                                  <  SRR4302540.4373436/94‑1 (MQ=60)
                                             GAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCC                                                  <  SRR4302540.4760837/94‑1 (MQ=60)
                                             GAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCC                                                  <  SRR4302540.6729567/94‑1 (MQ=60)
                                              AAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCT                                                 <  SRR4302540.4080948/94‑1 (MQ=60)
                                              AAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCT                                                 <  SRR4302540.4489608/94‑1 (MQ=60)
                                              AAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCT                                                 <  SRR4302540.3468285/94‑1 (MQ=60)
                                              AAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCT                                                 <  SRR4302540.4489609/94‑1 (MQ=60)
                                              AAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCT                                                 <  SRR4302540.9438472/94‑1 (MQ=60)
                                              AAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCT                                                 >  SRR4302540.9882503/1‑94 (MQ=60)
                                               AGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTG                                                <  SRR4302540.7909413/94‑1 (MQ=60)
                                               AGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTG                                                <  SRR4302540.7963935/94‑1 (MQ=60)
                                                GGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACGGCGTCTGTTTGAAGAAGTGCAACGATCCTGG                                               <  SRR4302540.9015471/94‑1 (MQ=60)
                                                 GTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGCCTGACTCTGTTTGAAGAAGTGCAACGATCCTGGA                                              >  SRR4302540.777790/1‑94 (MQ=60)
                                                 GTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGT                                              >  SRR4302540.348726/1‑94 (MQ=60)
                                                 GTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGT                                              >  SRR4302540.777793/1‑94 (MQ=60)
                                                 GTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGT                                              <  SRR4302540.6018827/94‑1 (MQ=60)
                                                  TGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTA                                             <  SRR4302540.9280419/94‑1 (MQ=60)
                                                  TGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTA                                             >  SRR4302540.9730929/1‑94 (MQ=60)
                                                   GATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTAC                                            >  SRR4302540.6047950/1‑94 (MQ=60)
                                                   GATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTAC                                            >  SRR4302540.676174/1‑94 (MQ=60)
                                                   GATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTAC                                            >  SRR4302540.4210523/1‑94 (MQ=60)
                                                   GATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGATTGAAGAAGTGCAACGATCCTGGTAC                                            >  SRR4302540.700638/1‑94 (MQ=60)
                                                    ATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACG                                           <  SRR4302540.5084074/94‑1 (MQ=60)
                                                     TCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGG                                          <  SRR4302540.213000/94‑1 (MQ=60)
                                                      CGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGA                                         >  SRR4302540.9365964/1‑94 (MQ=60)
                                                       GGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGTAACGATCCTGGTACGGAG                                        >  SRR4302540.9157318/1‑94 (MQ=60)
                                                       GGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGAC                                        >  SRR4302540.1954212/1‑94 (MQ=60)
                                                       GGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGAC                                        >  SRR4302540.3821167/1‑94 (MQ=60)
                                                       GGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGAC                                        >  SRR4302540.3743635/1‑94 (MQ=60)
                                                       GGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGAC                                        >  SRR4302540.8667899/1‑94 (MQ=60)
                                                        GGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACT                                       <  SRR4302540.4784034/94‑1 (MQ=60)
                                                         GCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTG                                      >  SRR4302540.9906371/1‑94 (MQ=60)
                                                         GCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTG                                      <  SRR4302540.8760230/94‑1 (MQ=60)
                                                          CTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGG                                     <  SRR4302540.3010678/94‑1 (MQ=60)
                                                          CTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGG                                     >  SRR4302540.10160620/1‑94 (MQ=60)
                                                           TGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGA                                    >  SRR4302540.6191775/1‑94 (MQ=60)
                                                           TGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGA                                    <  SRR4302540.2361323/94‑1 (MQ=60)
                                                           TGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGA                                    >  SRR4302540.6547189/1‑94 (MQ=60)
                                                            GAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGAT                                   <  SRR4302540.3113653/94‑1 (MQ=60)
                                                             AAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATC                                  <  SRR4302540.5520703/94‑1 (MQ=60)
                                                             AAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATC                                  <  SRR4302540.6957293/94‑1 (MQ=60)
                                                             AAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATC                                  <  SRR4302540.9482779/94‑1 (MQ=60)
                                                             AAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATC                                  >  SRR4302540.753633/1‑94 (MQ=60)
                                                             AAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATC                                  <  SRR4302540.7560109/94‑1 (MQ=60)
                                                             AAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATC                                  <  SRR4302540.3900197/94‑1 (MQ=60)
                                                             AAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATC                                  <  SRR4302540.4990359/94‑1 (MQ=60)
                                                              AATTGTTTGAGCGCGTGCGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCG                                 <  SRR4302540.1766391/94‑1 (MQ=60)
                                                              AATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCG                                 <  SRR4302540.2661052/94‑1 (MQ=60)
                                                              AATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCG                                 <  SRR4302540.1766390/94‑1 (MQ=60)
                                                              AANTGTTTGNGCGCGTACGTGNNCGNTTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCG                                 <  SRR4302540.612005/94‑1 (MQ=60)
                                                               ATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGC                                <  SRR4302540.5105259/94‑1 (MQ=60)
                                                               ATTGTTTGAGAGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGACTAAGTGCAACGATACTGGTACAGTCTGGATCGC                                >  SRR4302540.8053583/1‑94 (MQ=60)
                                                                TTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCA                               <  SRR4302540.3140903/94‑1 (MQ=60)
                                                                TTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCA                               >  SRR4302540.1336832/1‑94 (MQ=60)
                                                                TTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCA                               >  SRR4302540.1336833/1‑94 (MQ=60)
                                                                 TGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAT                              <  SRR4302540.3399227/94‑1 (MQ=60)
                                                                 TGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAT                              <  SRR4302540.7999650/94‑1 (MQ=60)
                                                                  GTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATT                             <  SRR4302540.8254105/94‑1 (MQ=60)
                                                                   TTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTG                            >  SRR4302540.2092987/1‑94 (MQ=60)
                                                                   TTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTG                            >  SRR4302540.6797882/1‑94 (MQ=60)
                                                                   GTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTG                            <  SRR4302540.3010314/94‑1 (MQ=60)
                                                                     TGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTC                          <  SRR4302540.8475293/94‑1 (MQ=60)
                                                                      GAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCA                         <  SRR4302540.5964602/94‑1 (MQ=60)
                                                                      GAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCA                         <  SRR4302540.3462238/94‑1 (MQ=60)
                                                                      GAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCA                         >  SRR4302540.5513635/1‑94 (MQ=60)
                                                                       AGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAG                        <  SRR4302540.4118773/94‑1 (MQ=60)
                                                                       AGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAG                        <  SRR4302540.2165431/94‑1 (MQ=60)
                                                                        GCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGC                       <  SRR4302540.4820547/94‑1 (MQ=60)
                                                                         CGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGCAGTGCAACGATCCTGGTACGGACTGGCTCGCATTGTCAGAG                      >  SRR4302540.9860988/1‑94 (MQ=60)
                                                                          GCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGC                     >  SRR4302540.8399680/1‑94 (MQ=60)
                                                                          GCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGC                     <  SRR4302540.1750471/94‑1 (MQ=60)
                                                                           CGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCC                    <  SRR4302540.8965468/94‑1 (MQ=60)
                                                                           CGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCC                    >  SRR4302540.6396800/1‑94 (MQ=60)
                                                                            GTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCG                   >  SRR4302540.5265383/1‑94 (MQ=60)
                                                                            GTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCG                   <  SRR4302540.5180091/94‑1 (MQ=60)
                                                                            GTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCG                   >  SRR4302540.1058854/1‑94 (MQ=60)
                                                                            GTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCG                   >  SRR4302540.1058856/1‑94 (MQ=60)
                                                                            GTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCG                   >  SRR4302540.4459666/1‑94 (MQ=60)
                                                                              ACGTGGGCGATTACTCCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCA                 <  SRR4302540.5324849/94‑1 (MQ=60)
                                                                               CGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAG                >  SRR4302540.3958884/1‑94 (MQ=60)
                                                                               CGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAG                <  SRR4302540.4013786/94‑1 (MQ=60)
                                                                                GTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTATGGACTGGATCGCATTGTCAGCGCCGCAGA               >  SRR4302540.7343203/1‑94 (MQ=60)
                                                                                GTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGA               <  SRR4302540.1149215/94‑1 (MQ=60)
                                                                                GTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGA               <  SRR4302540.2352494/94‑1 (MQ=60)
                                                                                 TGGGCGATTACACCCTCCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAA              <  SRR4302540.8624879/94‑1 (MQ=60)
                                                                                 TGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAA              <  SRR4302540.4798278/94‑1 (MQ=60)
                                                                                 TGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAA              >  SRR4302540.305919/1‑94 (MQ=60)
                                                                                  GGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAA             >  SRR4302540.1773779/1‑94 (MQ=60)
                                                                                  GGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAA             >  SRR4302540.96623/1‑94 (MQ=60)
                                                                                   GGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAG            <  SRR4302540.4875331/94‑1 (MQ=60)
                                                                                   GGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAG            <  SRR4302540.7811051/94‑1 (MQ=60)
                                                                                    GCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGT           <  SRR4302540.3372076/94‑1 (MQ=60)
                                                                                     CGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTC          <  SRR4302540.5020293/94‑1 (MQ=60)
                                                                                      GATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCT         <  SRR4302540.4027441/94‑1 (MQ=60)
                                                                                      GATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCT         >  SRR4302540.8324984/1‑94 (MQ=60)
                                                                                      GATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCT         <  SRR4302540.5950237/94‑1 (MQ=60)
                                                                                       ATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTG        <  SRR4302540.2817761/94‑1 (MQ=60)
                                                                                       ATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTG        >  SRR4302540.7963972/1‑94 (MQ=60)
                                                                                        TTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGC       <  SRR4302540.8409931/94‑1 (MQ=60)
                                                                                        TTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGC       <  SRR4302540.8449745/94‑1 (MQ=60)
                                                                                        TTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGC       <  SRR4302540.8449746/94‑1 (MQ=60)
                                                                                        TTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGC       >  SRR4302540.8613767/1‑94 (MQ=60)
                                                                                         TACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCG      <  SRR4302540.8976516/94‑1 (MQ=60)
                                                                                          ACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTCGATCGCATTGTCAGCGCCGCAGAAAGGCTGCGC     >  SRR4302540.319842/1‑94 (MQ=60)
                                                                                           CACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCG    <  SRR4302540.6295487/94‑1 (MQ=60)
                                                                                            ACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGG   >  SRR4302540.321195/1‑94 (MQ=60)
                                                                                             CCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAG  <  SRR4302540.866332/94‑1 (MQ=60)
                                                                                             CCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAG  >  SRR4302540.588906/1‑94 (MQ=60)
                                                                                             |                                                                                             
ACACACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAG  >  NC_000913/2977126‑2977312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑