Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 1624 250.6 12620048 98.0% 12367647 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,977,219 T→C H59H (CAT→CAC)  lysR → LysR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,977,2190TC99.6% 719.9 / ‑4.8 223H59H (CAT→CAC) lysRLysR
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (92/130);  total (93/130)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.58e-01

Only 100 of 228 total aligned reads displayed.

ACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGC  >  NC_000913/2977130‑2977309
                                                                                         |                                                                                          
  cTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTAc                                                                                       <  2:3397941/93‑1 (MQ=255)
  cTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTAc                                                                                       >  1:2009618/1‑93 (MQ=255)
  cTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTAc                                                                                       <  2:3155720/93‑1 (MQ=255)
   tCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTAcc                                                                                      >  1:4655846/1‑93 (MQ=255)
   tCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTAcc                                                                                      <  2:2768901/93‑1 (MQ=255)
          cAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAg                                                                               <  2:2061411/93‑1 (MQ=255)
          cAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAg                                                                               <  2:113557/93‑1 (MQ=255)
          cAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAg                                                                               <  2:5541244/93‑1 (MQ=255)
           aaCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAgg                                                                              >  2:5809451/1‑93 (MQ=255)
           aaCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAgg                                                                              >  1:481199/1‑93 (MQ=255)
           aaCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAgg                                                                              >  1:3242213/1‑93 (MQ=255)
            aCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGa                                                                             >  2:3732444/1‑93 (MQ=255)
            aCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGa                                                                             <  1:5183038/93‑1 (MQ=255)
            aCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGa                                                                             >  2:1975579/1‑93 (MQ=255)
              cGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACt                                                                           <  2:3479838/93‑1 (MQ=255)
               gTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTg                                                                          <  2:5334000/93‑1 (MQ=255)
               gTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTg                                                                          <  1:3218521/93‑1 (MQ=255)
               gTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTg                                                                          <  2:4842492/93‑1 (MQ=255)
                tCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGc                                                                         <  1:4042439/93‑1 (MQ=255)
                  aGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGt                                                                       >  2:2901590/1‑93 (MQ=255)
                   gCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTc                                                                      <  2:4769696/93‑1 (MQ=255)
                   gCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTc                                                                      >  2:3789394/1‑93 (MQ=255)
                   gCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTc                                                                      <  2:2555004/93‑1 (MQ=255)
                   gCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCATGCAAGGACTGCGTc                                                                      <  1:412193/93‑1 (MQ=255)
                     cgcgAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTg                                                                    >  1:4218971/1‑93 (MQ=255)
                      gcgAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGt                                                                   <  2:1481384/93‑1 (MQ=255)
                      gcgAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGt                                                                   <  2:5068122/93‑1 (MQ=255)
                      gcgAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGt                                                                   >  2:831525/1‑93 (MQ=255)
                        gAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTTTTTGAGCGCGTACGTGGGCGATTACACCCTACCGGGCAAGGACTGCGTCTGttt                                                                 <  2:5387869/93‑1 (MQ=255)
                        gAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGttt                                                                 >  1:3697097/1‑93 (MQ=255)
                           cTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTgaa                                                              >  1:5409535/1‑93 (MQ=255)
                           cTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTgaa                                                              >  1:5952118/1‑93 (MQ=255)
                            ttGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTgaag                                                             <  2:4686728/93‑1 (MQ=255)
                             tGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTgaaga                                                            >  1:4539597/1‑93 (MQ=255)
                             tGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTgaaga                                                            <  1:985447/93‑1 (MQ=255)
                               cgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGaagaag                                                          <  1:5786123/93‑1 (MQ=255)
                               cgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGCACTGCGTCTGTTTGaagaag                                                          <  2:6064910/93‑1 (MQ=255)
                                gcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGt                                                         >  2:5262254/1‑93 (MQ=255)
                                 cgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTg                                                        <  2:4384826/93‑1 (MQ=255)
                                  gcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGc                                                       <  2:1863876/93‑1 (MQ=255)
                                  gcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGc                                                       >  1:6012831/1‑93 (MQ=255)
                                   cTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCa                                                      <  1:2307380/93‑1 (MQ=255)
                                    tttGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCaa                                                     <  2:4332610/93‑1 (MQ=255)
                                     ttGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAAc                                                    <  2:3358331/93‑1 (MQ=255)
                                       gagaAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGa                                                  >  1:33494/1‑93 (MQ=255)
                                       gagaAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGa                                                  <  2:2489633/93‑1 (MQ=255)
                                         gaAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATc                                                >  2:2440870/1‑93 (MQ=255)
                                          aaGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATcc                                               <  1:5004418/93‑1 (MQ=255)
                                           aGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCt                                              <  2:107291/93‑1 (MQ=255)
                                              tGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGt                                           <  2:6093136/93‑1 (MQ=255)
                                               gATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGGAACGATCCTGGTa                                          >  2:1617741/1‑93 (MQ=255)
                                               gATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTa                                          <  1:2359631/93‑1 (MQ=255)
                                                aTCGGGCTGAAATTGTTTGAGCTCGTACGTGGGCGATTACGCCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAATTGCAACGATCCTGGTAc                                         <  2:4878776/93‑1 (MQ=255)
                                                aTCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTAc                                         >  2:1689898/1‑93 (MQ=255)
                                                aTCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTAc                                         >  1:2831835/1‑93 (MQ=255)
                                                 tCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACg                                        <  2:1248638/93‑1 (MQ=255)
                                                    ggCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGAc                                     <  2:4470804/93‑1 (MQ=255)
                                                     gCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACt                                    <  1:1024915/93‑1 (MQ=255)
                                                        gAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGa                                 >  1:5663343/1‑93 (MQ=255)
                                                         aaaTTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGAt                                >  1:5859667/1‑93 (MQ=255)
                                                         aaaTTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACAATCCTGGTACTGACTGGAt                                <  2:6107891/93‑1 (MQ=255)
                                                          aaTTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATc                               <  2:5514529/93‑1 (MQ=255)
                                                          aaTTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATc                               <  2:3619192/93‑1 (MQ=255)
                                                          aaTTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATc                               <  1:4619917/93‑1 (MQ=255)
                                                          aaTTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATc                               >  1:5223826/1‑93 (MQ=255)
                                                           aTTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCg                              >  2:4629341/1‑93 (MQ=255)
                                                             tGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCa                            <  2:4055920/93‑1 (MQ=255)
                                                              gTTTGAGCGCGTACGTGGGCGATTACCCCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAt                           <  1:4309468/93‑1 (MQ=255)
                                                              gTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAt                           <  1:4688504/93‑1 (MQ=255)
                                                              gTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAt                           >  1:513709/1‑93 (MQ=255)
                                                              gTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAt                           <  2:6264491/93‑1 (MQ=255)
                                                              gTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAt                           <  2:4726184/93‑1 (MQ=255)
                                                               tttGAGCGCGTACGTGGGCGATTACGCCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAtt                          <  2:950550/93‑1 (MQ=255)
                                                               tttGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAtt                          <  2:804385/93‑1 (MQ=255)
                                                                 tGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGt                        <  1:1460638/93‑1 (MQ=255)
                                                                  gAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTc                       <  2:1794303/93‑1 (MQ=255)
                                                                   aGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCa                      <  2:5702544/93‑1 (MQ=255)
                                                                   aGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTAGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCa                      >  2:6213215/1‑93 (MQ=255)
                                                                    gcgcGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAg                     >  1:2135378/1‑93 (MQ=255)
                                                                    gcgcGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAg                     <  1:2404981/93‑1 (MQ=255)
                                                                     cgcgTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAgc                    <  1:1597887/93‑1 (MQ=255)
                                                                        ttACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgcc                 <  1:297659/92‑1 (MQ=255)
                                                                        gTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgcc                 >  1:6136017/1‑93 (MQ=255)
                                                                        gTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgcc                 >  1:6217569/1‑93 (MQ=255)
                                                                        gTACGTGGGAGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgcc                 >  1:4078908/1‑93 (MQ=255)
                                                                         tACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgccg                >  2:5960868/1‑93 (MQ=255)
                                                                         tACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgccg                >  2:1759713/1‑93 (MQ=255)
                                                                         tACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgccg                >  1:1357434/1‑93 (MQ=255)
                                                                           cGTGGGCGATTTCACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTACAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCa              <  2:1479652/93‑1 (MQ=255)
                                                                              gggCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGaa           <  1:448156/93‑1 (MQ=255)
                                                                                 cGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGt        <  2:5007797/93‑1 (MQ=255)
                                                                                  gATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTc       <  1:3607186/93‑1 (MQ=255)
                                                                                  gATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTc       <  2:6240018/93‑1 (MQ=255)
                                                                                  gATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTc       <  2:2074229/93‑1 (MQ=255)
                                                                                   aTTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCt      >  1:1700628/1‑93 (MQ=255)
                                                                                    ttACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTg     >  1:1889655/1‑93 (MQ=255)
                                                                                    ttACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTg     <  1:6165707/93‑1 (MQ=255)
                                                                                     tACACCCTCCCGTGCAAGTACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGTACTGGATTGCATTGTCACCGCCGCAGAAAGTCTgc    <  2:3042772/93‑1 (MQ=255)
                                                                                     tACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTgc    <  1:547144/93‑1 (MQ=255)
                                                                                     tACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTgc    >  2:199938/1‑93 (MQ=255)
                                                                                         |                                                                                          
ACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGC  >  NC_000913/2977130‑2977309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CACACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAG  >  NC_000913/2977127‑2977312
                                                                                            |                                                                                             
CACACCTCACAGCCAACCGTCAGCCGCGAGCTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACC                                                                                              <  SRR4302533.3301814/94‑1 (MQ=60)
CACACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACC                                                                                              >  SRR4302533.5207346/1‑94 (MQ=60)
 ACACCTCACACCCAACCGTCAGCCGCGAACTTGCGCTCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCC                                                                                             <  SRR4302533.997591/94‑1 (MQ=60)
  CACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCT                                                                                            >  SRR4302533.3705647/1‑94 (MQ=60)
    CCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTAC                                                                                          >  SRR4302533.2021996/1‑94 (MQ=60)
    CCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTAC                                                                                          >  SRR4302533.2190728/1‑94 (MQ=60)
     CTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACC                                                                                         >  SRR4302533.4684058/1‑94 (MQ=60)
      TCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCG                                                                                        <  SRR4302533.545377/94‑1 (MQ=60)
       CACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGT                                                                                       <  SRR4302533.5018531/94‑1 (MQ=60)
         CAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGC                                                                                     >  SRR4302533.2124072/1‑94 (MQ=60)
          AGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCA                                                                                    <  SRR4302533.4095755/94‑1 (MQ=60)
            CCAGCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAG                                                                                  <  SRR4302533.4056910/94‑1 (MQ=60)
             CAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGG                                                                                 >  SRR4302533.484074/1‑94 (MQ=60)
             CAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGG                                                                                 >  SRR4302533.3262194/1‑94 (MQ=60)
              AACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGA                                                                                >  SRR4302533.2389738/1‑94 (MQ=60)
              AACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTCAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGA                                                                                >  SRR4302533.1148421/1‑94 (MQ=60)
               ACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGAC                                                                               >  SRR4302533.3431439/1‑94 (MQ=60)
               ACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGAC                                                                               <  SRR4302533.5214331/94‑1 (MQ=60)
                  GTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGC                                                                            <  SRR4302533.3238366/94‑1 (MQ=60)
                   TCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCG                                                                           >  SRR4302533.3288913/1‑94 (MQ=60)
                   TCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCG                                                                           <  SRR4302533.915936/94‑1 (MQ=60)
                   TCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCG                                                                           <  SRR4302533.4067043/94‑1 (MQ=60)
                     AGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTC                                                                         >  SRR4302533.4705014/1‑94 (MQ=60)
                      GCCGCGAACTTGCGCGCTTTGAGAAGTTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCT                                                                        <  SRR4302533.5764137/94‑1 (MQ=60)
                      GCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCATGCAAGGACTGCGTCT                                                                        <  SRR4302533.414667/94‑1 (MQ=60)
                       CCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTG                                                                       >  SRR4302533.4244486/1‑94 (MQ=60)
                        CGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGT                                                                      <  SRR4302533.4632294/94‑1 (MQ=60)
                        CGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGT                                                                      >  SRR4302533.2475900/1‑94 (MQ=60)
                          CGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTT                                                                    >  SRR4302533.3719809/1‑94 (MQ=60)
                           GAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTG                                                                   >  SRR4302533.588024/1‑94 (MQ=60)
                            AACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGA                                                                  >  SRR4302533.4527939/1‑94 (MQ=60)
                             ACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAA                                                                 >  SRR4302533.5442051/1‑94 (MQ=60)
                             ACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAA                                                                 >  SRR4302533.5988122/1‑94 (MQ=60)
                               TTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGA                                                               >  SRR4302533.4567082/1‑94 (MQ=60)
                                TGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAA                                                              <  SRR4302533.991217/94‑1 (MQ=60)
                                TGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAA                                                              >  SRR4302533.5899983/1‑94 (MQ=60)
                                  CGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGT                                                            <  SRR4302533.5821072/94‑1 (MQ=60)
                                    CGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGC                                                          >  SRR4302533.6049259/1‑94 (MQ=60)
                                      CTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAA                                                        <  SRR4302533.2321674/94‑1 (MQ=60)
                                       TTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAAC                                                       <  SRR4302533.5043091/94‑1 (MQ=60)
                                        TTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACG                                                      <  SRR4302533.3288979/94‑1 (MQ=60)
                                        TTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACG                                                      >  SRR4302533.6113346/1‑94 (MQ=60)
                                         TGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGA                                                     >  SRR4302533.33722/1‑94 (MQ=60)
                                         TGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGA                                                     >  SRR4302533.3244755/1‑94 (MQ=60)
                                          GAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGAT                                                    >  SRR4302533.4676243/1‑94 (MQ=60)
                                          GAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGAT                                                    <  SRR4302533.4107290/94‑1 (MQ=60)
                                            GAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCC                                                  <  SRR4302533.4263335/94‑1 (MQ=60)
                                             AAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCT                                                 <  SRR4302533.5034747/94‑1 (MQ=60)
                                             AAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCT                                                 <  SRR4302533.1537719/94‑1 (MQ=60)
                                              AGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTG                                                >  SRR4302533.5404883/1‑94 (MQ=60)
                                               GGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGG                                               <  SRR4302533.1416114/94‑1 (MQ=60)
                                                  GATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTAC                                            <  SRR4302533.2374214/94‑1 (MQ=60)
                                                  GATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTAC                                            >  SRR4302533.2849258/1‑94 (MQ=60)
                                                  GATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTAC                                            <  SRR4302533.4781436/94‑1 (MQ=60)
                                                    TCGGTGTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGG                                          <  SRR4302533.2907768/94‑1 (MQ=60)
                                                     CGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGA                                         >  SRR4302533.4843574/1‑94 (MQ=60)
                                                       GGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACT                                       <  SRR4302533.5181849/94‑1 (MQ=60)
                                                       GGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCATGCCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACT                                       <  SRR4302533.2370713/94‑1 (MQ=60)
                                                        GCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTG                                      <  SRR4302533.1030940/94‑1 (MQ=60)
                                                        GCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTG                                      <  SRR4302533.5248866/94‑1 (MQ=60)
                                                          TGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGA                                    >  SRR4302533.61815/1‑94 (MQ=60)
                                                          TGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGA                                    >  SRR4302533.5697561/1‑94 (MQ=60)
                                                          TGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGA                                    >  SRR4302533.383565/1‑94 (MQ=60)
                                                           GAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGAT                                   >  SRR4302533.5895090/1‑94 (MQ=60)
                                                           GAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGAT                                   <  SRR4302533.2575913/94‑1 (MQ=60)
                                                            AAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATC                                  <  SRR4302533.1641832/94‑1 (MQ=60)
                                                            AAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATC                                  >  SRR4302533.654411/1‑94 (MQ=60)
                                                            AAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATC                                  >  SRR4302533.5255252/1‑94 (MQ=60)
                                                            AAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATC                                  <  SRR4302533.5766326/94‑1 (MQ=60)
                                                             AATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCG                                 <  SRR4302533.4647915/94‑1 (MQ=60)
                                                                TGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAT                              >  SRR4302533.516762/1‑94 (MQ=60)
                                                                TGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAT                              >  SRR4302533.5624165/1‑94 (MQ=60)
                                                                 GTTTGAGCGCGTACGTGGGCGATTACCCCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATT                             <  SRR4302533.4335410/94‑1 (MQ=60)
                                                                 GTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATT                             <  SRR4302533.4716926/94‑1 (MQ=60)
                                                                    TGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTC                          <  SRR4302533.1469441/94‑1 (MQ=60)
                                                                    TGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTC                          <  SRR4302533.1641196/94‑1 (MQ=60)
                                                                     GAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCA                         <  SRR4302533.6026086/94‑1 (MQ=60)
                                                                      AGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAG                        <  SRR4302533.4224903/94‑1 (MQ=60)
                                                                      AGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAG                        >  SRR4302533.2148544/1‑94 (MQ=60)
                                                                      AGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAG                        >  SRR4302533.1998482/1‑94 (MQ=60)
                                                                       GCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGC                       <  SRR4302533.2419832/94‑1 (MQ=60)
                                                                       GCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGC                       <  SRR4302533.4333821/94‑1 (MQ=60)
                                                                        CGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCG                      <  SRR4302533.1607561/94‑1 (MQ=60)
                                                                         GCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGC                     >  SRR4302533.6120597/1‑94 (MQ=60)
                                                                          CGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCC                    >  SRR4302533.6172986/1‑94 (MQ=60)
                                                                          CGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCC                    >  SRR4302533.6254851/1‑94 (MQ=60)
                                                                          CGTACGTGGGAGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCC                    >  SRR4302533.4103739/1‑94 (MQ=60)
                                                                           TTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCG                   <  SRR4302533.299482/94‑1 (MQ=60)
                                                                           GTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCG                   >  SRR4302533.1747069/1‑94 (MQ=60)
                                                                           GTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCG                   >  SRR4302533.1365592/1‑94 (MQ=60)
                                                                               GTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGA               >  SRR4302533.6245673/1‑94 (MQ=60)
                                                                                 GGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAGCGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAA             <  SRR4302533.4472386/94‑1 (MQ=60)
                                                                                 GGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAA             <  SRR4302533.450835/94‑1 (MQ=60)
                                                                                  GGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAG            >  SRR4302533.3236398/1‑94 (MQ=60)
                                                                                  GGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAG            <  SRR4302533.2895918/94‑1 (MQ=60)
                                                                                    CGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTC          <  SRR4302533.4642316/94‑1 (MQ=60)
                                                                                     GATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCT         >  SRR4302533.1710936/1‑94 (MQ=60)
                                                                                     GATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCT         <  SRR4302533.3629392/94‑1 (MQ=60)
                                                                                     GATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCT         >  SRR4302533.5377260/1‑94 (MQ=60)
                                                                                      ATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTG        >  SRR4302533.1901195/1‑94 (MQ=60)
                                                                                       TTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGC       <  SRR4302533.4684175/94‑1 (MQ=60)
                                                                                       TTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGC       <  SRR4302533.6202768/94‑1 (MQ=60)
                                                                                        TACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCG      <  SRR4302533.550393/94‑1 (MQ=60)
                                                                                         ACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGC     >  SRR4302533.2301761/1‑94 (MQ=60)
                                                                                            CCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAG  >  SRR4302533.4274412/1‑94 (MQ=60)
                                                                                            |                                                                                             
CACACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGCGAG  >  NC_000913/2977127‑2977312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑