Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,964,041 A→G T4A (ACC→GCC)  rho → transcription termination factor Rho

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,964,0410AG99.8% 2000.7 / ‑5.2 615T4A (ACC→GCC) rhotranscription termination factor Rho
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (290/323);  total (290/325)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-01

Only 100 of 625 total aligned reads displayed.

TAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGAC  >  NC_000913/3963951‑3964130
                                                                                          |                                                                                         
tAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGcc                                                                                         <  1:3350399/93‑1 (MQ=255)
tAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGcc                                                                                         >  1:2918288/1‑93 (MQ=255)
  aaCAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGa                                                                                       <  1:2288190/93‑1 (MQ=255)
  aaCAGGCAGGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGa                                                                                       <  1:8341809/93‑1 (MQ=255)
    aaGGCATGGATGTCCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAAt                                                                                     <  2:2748744/92‑1 (MQ=255)
     aGGCATGGATGATCCTGCCATAACATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACAACTATGAATCTTGCCGAAta                                                                                    >  2:2520868/1‑92 (MQ=255)
      ggCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTa                                                                                   <  2:314099/93‑1 (MQ=255)
         aTGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAg                                                                                >  2:7225216/1‑93 (MQ=255)
           ggATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGaa                                                                              >  1:6991287/1‑93 (MQ=255)
           ggATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGaa                                                                              <  2:5865833/93‑1 (MQ=255)
           ggATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGaa                                                                              <  2:3605121/93‑1 (MQ=255)
           gaatgatCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGaa                                                                              <  1:9694003/91‑1 (MQ=255)
            gatgatCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAAt                                                                             >  2:6838883/1‑93 (MQ=255)
            gatgatCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAAt                                                                             <  2:4450557/93‑1 (MQ=255)
             atgatCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATa                                                                            <  2:9542679/93‑1 (MQ=255)
             atgatCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATa                                                                            <  2:5355279/93‑1 (MQ=255)
             atgatCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATa                                                                            <  2:6775977/93‑1 (MQ=255)
              tgatCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATAc                                                                           >  1:6849293/1‑93 (MQ=255)
                atCCTGCCATACCATTCACAACATTAAGTTCGAGGTTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGc                                                                         <  2:7678774/93‑1 (MQ=255)
                atCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGc                                                                         <  1:396120/93‑1 (MQ=255)
                atCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGc                                                                         >  2:8604860/1‑93 (MQ=255)
                    tGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGt                                                                     <  1:3432456/93‑1 (MQ=255)
                    tGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGt                                                                     >  2:6984212/1‑93 (MQ=255)
                     gCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGtt                                                                    >  1:756485/1‑93 (MQ=255)
                       cATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTc                                                                  <  2:3321923/93‑1 (MQ=255)
                       cATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTc                                                                  <  2:7921263/93‑1 (MQ=255)
                        aTACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCt                                                                 <  1:8787412/93‑1 (MQ=255)
                        aTACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCt                                                                 <  1:5106546/93‑1 (MQ=255)
                         tACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTg                                                                >  2:5676602/1‑93 (MQ=255)
                         tACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTg                                                                >  1:7690138/1‑93 (MQ=255)
                         tACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTg                                                                >  2:4301318/1‑93 (MQ=255)
                         tACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTg                                                                >  1:818018/1‑93 (MQ=255)
                           ccATTCACAACATTAAGTTCGAGATTTACCCCAAGGTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAg                                                              >  1:6345120/1‑93 (MQ=255)
                            cATTCACAACATTAAATTCGATATTTACTCCAAGTTTAAGAACTCACACCACCATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGc                                                             <  2:337678/93‑1 (MQ=255)
                               tCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGa                                                          >  1:4628745/1‑93 (MQ=255)
                               tCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGa                                                          >  1:8673125/1‑93 (MQ=255)
                                cacaACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGAt                                                         >  2:7573436/1‑93 (MQ=255)
                                 acaacaTTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATc                                                        <  1:10006156/93‑1 (MQ=255)
                                 acaacaTTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATc                                                        >  2:1845586/1‑93 (MQ=255)
                                 acaacaTTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATc                                                        <  1:9183698/93‑1 (MQ=255)
                                 acaacaTTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATc                                                        <  1:7997476/93‑1 (MQ=255)
                                    acaTTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCAct                                                     >  1:7268890/1‑93 (MQ=255)
                                    acaTTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCAct                                                     >  2:1541812/1‑93 (MQ=255)
                                     caTTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCActc                                                    <  1:1269297/93‑1 (MQ=255)
                                      aTTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCActct                                                   >  2:4599067/1‑93 (MQ=255)
                                      aTTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCActct                                                   >  2:2345099/1‑93 (MQ=255)
                                       ttAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACtctc                                                  >  1:3650061/1‑93 (MQ=255)
                                          aGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGc                                               <  1:2406558/93‑1 (MQ=255)
                                          aGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGc                                               <  1:3255191/93‑1 (MQ=255)
                                            ttCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGa                                             >  2:2212192/1‑93 (MQ=255)
                                              cGAGATTTACCCCAAGTAAAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGaaa                                           <  2:6291114/93‑1 (MQ=255)
                                               gagaTTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGaaaa                                          >  1:3875400/1‑93 (MQ=255)
                                                 gaTTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAata                                        <  1:7854600/93‑1 (MQ=255)
                                                 gaTTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAata                                        <  1:3509634/93‑1 (MQ=255)
                                                 gaTTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAata                                        <  1:667680/93‑1 (MQ=255)
                                                 gaTTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTCAGCTGATCACTCTCGGCGAAActa                                        >  1:1444238/1‑93 (MQ=255)
                                                 aaTTTTCCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCCCTCTCGGCGAAAata                                        <  2:6499923/92‑1 (MQ=255)
                                                  aTTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCTAAAatat                                       <  2:1698852/93‑1 (MQ=255)
                                                  aTTTACCCCAAGTTTAAGAACTCACACCACTACGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAatat                                       >  1:8971673/1‑93 (MQ=255)
                                                   tttACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATg                                      <  2:6137219/93‑1 (MQ=255)
                                                   tttACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATg                                      >  2:1438602/1‑93 (MQ=255)
                                                   tttACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATg                                      >  1:5504414/1‑93 (MQ=255)
                                                   tttACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATg                                      <  1:1556346/93‑1 (MQ=255)
                                                    ttACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATgg                                     >  2:7433124/1‑93 (MQ=255)
                                                     tACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATggg                                    <  2:6660011/93‑1 (MQ=255)
                                                      tccccAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATgggg                                   <  1:1832153/92‑1 (MQ=255)
                                                       ccccAAGTTTAAGACCTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGc                                  <  2:8812021/93‑1 (MQ=255)
                                                        cccAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCt                                 >  2:30232/1‑93 (MQ=255)
                                                        cccAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCt                                 >  2:9155742/1‑93 (MQ=255)
                                                         ccAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTg                                <  1:596432/93‑1 (MQ=255)
                                                         ccAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTg                                >  2:3190076/1‑93 (MQ=255)
                                                           aaGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGa                              <  1:5744826/93‑1 (MQ=255)
                                                            aGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCGCTCTCGGCGAAAATATGGGGCTGGaa                             >  1:4906479/1‑93 (MQ=255)
                                                             gTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGgaa                            >  1:94704/1‑93 (MQ=255)
                                                             gTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGaaa                            >  2:4922593/1‑93 (MQ=255)
                                                              tttAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGaaaa                           >  1:4248784/1‑93 (MQ=255)
                                                               ttAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACTCCGGGTTCTGAGCTGATACCTCTCGGCCAAAAGATGTGGCTGCAAAAc                          >  1:5319785/1‑93 (MQ=255)
                                                               ttAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAAc                          <  2:1339845/93‑1 (MQ=255)
                                                                    aaCTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGc                     >  1:2706622/1‑93 (MQ=255)
                                                                      cTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAGTATGGGGCTGGAAAACCTGGCTc                   >  2:5501252/1‑93 (MQ=255)
                                                                      cTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTc                   <  1:2140883/93‑1 (MQ=255)
                                                                      cTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTc                   <  2:8271898/93‑1 (MQ=255)
                                                                       tCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCg                  >  2:1131475/1‑93 (MQ=255)
                                                                        cacaCCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGt                 <  2:9814707/93‑1 (MQ=255)
                                                                         ccaccacTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTa                <  2:2031360/92‑1 (MQ=255)
                                                                         acacCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTa                <  2:8048035/93‑1 (MQ=255)
                                                                         acacCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTa                <  1:9115534/93‑1 (MQ=255)
                                                                          caccacTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTAt               <  1:1139098/93‑1 (MQ=255)
                                                                             cacTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCg            <  1:586507/93‑1 (MQ=255)
                                                                               cTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTa          <  2:4098778/93‑1 (MQ=255)
                                                                               cTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTa          <  2:3887550/93‑1 (MQ=255)
                                                                                tATGCATCTTGCAGACTTATAGAATACGCCGGTTTCTGAGCTGATCAATCTCGGCGAAAATATGGGGCTGGAAAAACTGGCTCATTTGCGTaa         <  2:8977891/93‑1 (MQ=255)
                                                                                tATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTaa         >  1:8475358/1‑93 (MQ=255)
                                                                                  tGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGc       >  2:2924091/1‑93 (MQ=255)
                                                                                  tGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGc       <  1:1706764/93‑1 (MQ=255)
                                                                                   gAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCa      >  1:1974121/1‑93 (MQ=255)
                                                                                     aTCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAgg    <  1:9912109/93‑1 (MQ=255)
                                                                                     aTCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAgg    >  2:9686057/1‑93 (MQ=255)
                                                                                      tCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGa   <  2:5048018/93‑1 (MQ=255)
                                                                                      tCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGa   <  2:9369534/93‑1 (MQ=255)
                                                                                          |                                                                                         
TAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGAC  >  NC_000913/3963951‑3964130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 330 total aligned reads displayed.
CCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTA  >  NC_000913/3963948‑3964134
                                                                                             |                                                                                             
CCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTG                                                                                               <  SRR4302540.3573800/94‑1 (MQ=60)
CCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTG                                                                                               <  SRR4302540.756474/94‑1 (MQ=60)
 CGTAATCAGGCAGGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGC                                                                                              <  SRR4302540.7978251/94‑1 (MQ=60)
  aaAAACAGGAATGAATGACCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCC                                                                                             <  SRR4302540.7248431/92‑1 (MQ=60)
  GTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCC                                                                                             >  SRR4302540.2950240/1‑94 (MQ=60)
  GTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCC                                                                                             >  SRR4302540.4866924/1‑94 (MQ=60)
  GTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCC                                                                                             >  SRR4302540.4956756/1‑94 (MQ=60)
  GTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCC                                                                                             >  SRR4302540.9048404/1‑94 (MQ=60)
  GTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCC                                                                                             >  SRR4302540.5662920/1‑94 (MQ=60)
  GTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCC                                                                                             <  SRR4302540.5739106/94‑1 (MQ=60)
  GTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCC                                                                                             >  SRR4302540.3433179/1‑94 (MQ=60)
    AAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGA                                                                                           >  SRR4302540.9242248/1‑94 (MQ=60)
    AAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGA                                                                                           >  SRR4302540.1480156/1‑94 (MQ=60)
     AACAGGCATGTATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAA                                                                                          <  SRR4302540.1154893/94‑1 (MQ=60)
     AACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAA                                                                                          <  SRR4302540.7711894/94‑1 (MQ=60)
     AACAGGCAGGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAA                                                                                          <  SRR4302540.8438633/94‑1 (MQ=60)
      ACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAAT                                                                                         <  SRR4302540.1822632/94‑1 (MQ=60)
        AGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTA                                                                                       >  SRR4302540.8141525/1‑94 (MQ=60)
         GTCATGGATGACCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAA                                                                                      <  SRR4302540.3720741/94‑1 (MQ=60)
         GGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAA                                                                                      >  SRR4302540.3562469/1‑94 (MQ=60)
            ATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGA                                                                                   >  SRR4302540.9156068/1‑94 (MQ=60)
            ATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGA                                                                                   >  SRR4302540.4463272/1‑94 (MQ=60)
            ATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGA                                                                                   >  SRR4302540.2168806/1‑94 (MQ=60)
            ATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGA                                                                                   >  SRR4302540.2463312/1‑94 (MQ=60)
            ATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGA                                                                                   >  SRR4302540.1817381/1‑94 (MQ=60)
            ATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGA                                                                                   >  SRR4302540.5724294/1‑94 (MQ=60)
            ATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGA                                                                                   >  SRR4302540.6530734/1‑94 (MQ=60)
            ATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGA                                                                                   >  SRR4302540.1405927/1‑94 (MQ=60)
             TGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAA                                                                                  <  SRR4302540.5962685/94‑1 (MQ=60)
             TGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAA                                                                                  >  SRR4302540.2117099/1‑94 (MQ=60)
             TGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAA                                                                                  <  SRR4302540.8338087/94‑1 (MQ=60)
             TGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCACAACTTTAAGAACTCACAACAATATGAATCTTGCCGAATTAAACAA                                                                                  >  SRR4302540.7068889/1‑94 (MQ=60)
             TGAATGAGCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAA                                                                                  <  SRR4302540.3044154/94‑1 (MQ=60)
              GGATGATCCTGCCATACCATTCACAACATTAAGATCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAAT                                                                                 <  SRR4302540.9808013/94‑1 (MQ=60)
              GGATGACCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAAT                                                                                 <  SRR4302540.5900414/94‑1 (MQ=60)
               GATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATA                                                                                <  SRR4302540.4221582/94‑1 (MQ=60)
               GATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATA                                                                                >  SRR4302540.6647443/1‑94 (MQ=60)
                ATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATAC                                                                               <  SRR4302540.6550617/94‑1 (MQ=60)
                ATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATAC                                                                               <  SRR4302540.3504825/94‑1 (MQ=60)
                 TGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACG                                                                              >  SRR4302540.8384073/1‑94 (MQ=60)
                 TGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACG                                                                              <  SRR4302540.167862/94‑1 (MQ=60)
                 TGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACG                                                                              >  SRR4302540.9670364/1‑94 (MQ=60)
                 TGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACG                                                                              <  SRR4302540.167861/94‑1 (MQ=60)
                  GATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGC                                                                             >  SRR4302540.9118408/1‑94 (MQ=60)
                  GATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGC                                                                             >  SRR4302540.9721578/1‑94 (MQ=60)
                   ATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCC                                                                            <  SRR4302540.8867667/94‑1 (MQ=60)
                   ACCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCC                                                                            <  SRR4302540.5280804/94‑1 (MQ=60)
                    TCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCG                                                                           <  SRR4302540.4698003/94‑1 (MQ=60)
                    CCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCG                                                                           <  SRR4302540.1900694/94‑1 (MQ=60)
                     CCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGG                                                                          <  SRR4302540.8321351/94‑1 (MQ=60)
                     CCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGG                                                                          <  SRR4302540.8321352/94‑1 (MQ=60)
                       TGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTT                                                                        >  SRR4302540.764543/1‑94 (MQ=60)
                        GCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTT                                                                       <  SRR4302540.2563363/94‑1 (MQ=60)
                        GCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTT                                                                       >  SRR4302540.3901308/1‑94 (MQ=60)
                        GCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTT                                                                       <  SRR4302540.7143731/94‑1 (MQ=60)
                         CCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTC                                                                      >  SRR4302540.3147625/1‑94 (MQ=60)
                         CCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTC                                                                      >  SRR4302540.5993416/1‑94 (MQ=60)
                         CCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTC                                                                      >  SRR4302540.331432/1‑94 (MQ=60)
                          CATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCT                                                                     <  SRR4302540.2967091/94‑1 (MQ=60)
                          CAGACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCCTGCCGAATTAAAGAATACGCCGGTTgcg                                                                     >  SRR4302540.9071395/1‑91 (MQ=60)
                           ATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCATACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTG                                                                    >  SRR4302540.8551576/1‑94 (MQ=60)
                           ATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTG                                                                    <  SRR4302540.8889098/94‑1 (MQ=60)
                           ATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTG                                                                    >  SRR4302540.20746/1‑94 (MQ=60)
                           ATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTG                                                                    >  SRR4302540.7702352/1‑94 (MQ=60)
                           ATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTG                                                                    >  SRR4302540.5297502/1‑94 (MQ=60)
                           ATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTG                                                                    >  SRR4302540.826710/1‑94 (MQ=60)
                           ATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTG                                                                    >  SRR4302540.9839491/1‑94 (MQ=60)
                           ATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCAGTTTCTG                                                                    <  SRR4302540.112651/94‑1 (MQ=60)
                             ACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAG                                                                  >  SRR4302540.517224/1‑94 (MQ=60)
                             ACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAG                                                                  <  SRR4302540.5378498/94‑1 (MQ=60)
                             ACCATTCACAACATTAAGTTCGAGATTTACCCCAAGGTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAG                                                                  >  SRR4302540.6415775/1‑94 (MQ=60)
                              CCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGC                                                                 >  SRR4302540.9139242/1‑94 (MQ=60)
                              CCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGC                                                                 >  SRR4302540.2367739/1‑94 (MQ=60)
                              CCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGC                                                                 >  SRR4302540.1121534/1‑94 (MQ=60)
                               CATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCT                                                                >  SRR4302540.1965595/1‑94 (MQ=60)
                               CATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCT                                                                >  SRR4302540.9230365/1‑94 (MQ=60)
                               CATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCT                                                                <  SRR4302540.1718740/94‑1 (MQ=60)
                                ATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTG                                                               >  SRR4302540.1944955/1‑94 (MQ=60)
                                 TTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGA                                                              <  SRR4302540.5903470/94‑1 (MQ=60)
                                 TTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGA                                                              >  SRR4302540.8773867/1‑94 (MQ=60)
                                 TTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGA                                                              >  SRR4302540.8960889/1‑94 (MQ=60)
                                 TTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGA                                                              >  SRR4302540.4678329/1‑94 (MQ=60)
                                  tgccaaaactaacttcgaaagttgccccaatggttAGAACTCACACCACTATGAATCTTGCCAAATGAAAGAATACTCCGGGTTCTGAGCTGAT                                                             <  SRR4302540.7300417/59‑1 (MQ=34)
                                  TCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGAT                                                             <  SRR4302540.7300418/94‑1 (MQ=60)
                                   CACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATC                                                            <  SRR4302540.949859/94‑1 (MQ=60)
                                   CACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATC                                                            <  SRR4302540.3474567/94‑1 (MQ=60)
                                   CACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATC                                                            <  SRR4302540.9501925/94‑1 (MQ=60)
                                   CACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATC                                                            <  SRR4302540.5960280/94‑1 (MQ=60)
                                    ACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCA                                                           <  SRR4302540.8090570/94‑1 (MQ=60)
                                     CAACATTAAGTTCGAGATTTACCCCAAGTTTAAGACCTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCAC                                                          <  SRR4302540.9620492/94‑1 (MQ=60)
                                      AACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACT                                                         >  SRR4302540.7194238/1‑94 (MQ=60)
                                      AACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACT                                                         >  SRR4302540.7350003/1‑94 (MQ=60)
                                        CATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCT                                                       >  SRR4302540.3397422/1‑94 (MQ=60)
                                         ATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTC                                                      <  SRR4302540.8668018/94‑1 (MQ=60)
                                         ATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTC                                                      <  SRR4302540.6760064/94‑1 (MQ=60)
                                         ATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTC                                                      >  SRR4302540.3690049/1‑94 (MQ=60)
                                          TTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCG                                                     <  SRR4302540.3393686/94‑1 (MQ=60)
                                          TTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCG                                                     <  SRR4302540.9123508/94‑1 (MQ=60)
                                           TAAGTTCGAGATTTACCCCAAGTTTAAGACCTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGG                                                    <  SRR4302540.928578/94‑1 (MQ=60)
                                            AAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGC                                                   >  SRR4302540.3505813/1‑94 (MQ=60)
                                            AAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGC                                                   >  SRR4302540.7914765/1‑94 (MQ=60)
                                             AGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCG                                                  <  SRR4302540.2432505/94‑1 (MQ=60)
                                             AGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCG                                                  <  SRR4302540.3318680/94‑1 (MQ=60)
                                             AGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCG                                                  <  SRR4302540.5402357/94‑1 (MQ=60)
                                             AGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCG                                                  <  SRR4302540.3290673/94‑1 (MQ=60)
                                                 CGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAA                                              >  SRR4302540.3917521/1‑94 (MQ=60)
                                                 CGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAAGCTTGTCGAATTAAAGAATGCGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAA                                              <  SRR4302540.10128056/94‑1 (MQ=60)
                                                  GAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAAT                                             <  SRR4302540.5166732/94‑1 (MQ=60)
                                                   AGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATA                                            >  SRR4302540.8030690/1‑94 (MQ=60)
                                                   AGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATA                                            >  SRR4302540.1459939/1‑94 (MQ=60)
                                                   AGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTCAGCTGATCACTCTCGGCGAAACTA                                            >  SRR4302540.1459938/1‑94 (MQ=60)
                                                    gatctccccccagtgTAAGACCTCACACCACTATGAGTCTTGCCGAATTAAAGAATACGCCGGTTTCTGACCTGATCACTCTCGGCGAAAATAT                                           <  SRR4302540.5011095/79‑1 (MQ=60)
                                                    GATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATAT                                           <  SRR4302540.674839/94‑1 (MQ=60)
                                                    GATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATAT                                           <  SRR4302540.10163015/94‑1 (MQ=60)
                                                    GATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATAT                                           <  SRR4302540.7946379/94‑1 (MQ=60)
                                                     ATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATG                                          >  SRR4302540.5565038/1‑94 (MQ=60)
                                                     ATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATG                                          <  SRR4302540.8481606/94‑1 (MQ=60)
                                                      TTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGG                                         <  SRR4302540.2378016/94‑1 (MQ=60)
                                                      TTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGG                                         <  SRR4302540.1573454/94‑1 (MQ=60)
                                                      TTNANCNCANGTTTAAGAACTNNCANCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGG                                         <  SRR4302540.457874/94‑1 (MQ=60)
                                                       TTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGG                                        >  SRR4302540.1551210/1‑94 (MQ=60)
                                                       TTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGG                                        <  SRR4302540.6832781/94‑1 (MQ=60)
                                                       TTACCCCAAGGTTAAGAACTCACACCACTATGAATTTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGG                                        <  SRR4302540.6832782/94‑1 (MQ=60)
                                                        TACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGG                                       <  SRR4302540.3541345/94‑1 (MQ=60)
                                                        TACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGG                                       <  SRR4302540.4910444/94‑1 (MQ=60)
                                                        TACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGG                                       <  SRR4302540.5135306/94‑1 (MQ=60)
                                                         TCCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGC                                      <  SRR4302540.1852198/94‑1 (MQ=60)
                                                         ACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGC                                      >  SRR4302540.233440/1‑94 (MQ=60)
                                                         ACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGC                                      <  SRR4302540.8592495/94‑1 (MQ=60)
                                                           CCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTG                                    <  SRR4302540.4010231/94‑1 (MQ=60)
                                                            CCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGG                                   <  SRR4302540.2913386/94‑1 (MQ=60)
                                                            CCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGG                                   <  SRR4302540.1354957/94‑1 (MQ=60)
                                                            CCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGG                                   >  SRR4302540.385297/1‑94 (MQ=60)
                                                            CCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGG                                   <  SRR4302540.602836/94‑1 (MQ=60)
                                                            CCAAGTGTAAAAACTCACACCACTATGAATCTTCACGAATTAAAGAATACGCCGGTTTCTGAGCTGATCCCTCTCGGCGAAAATATGGGGCTGG                                   <  SRR4302540.2913385/94‑1 (MQ=60)
                                                             CAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGA                                  <  SRR4302540.2082276/94‑1 (MQ=60)
                                                             CAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGA                                  >  SRR4302540.5626320/1‑94 (MQ=60)
                                                              AAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAA                                 >  SRR4302540.1714394/1‑94 (MQ=60)
                                                              AAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAA                                 <  SRR4302540.5032626/94‑1 (MQ=60)
                                                              AAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAA                                 >  SRR4302540.2927587/1‑94 (MQ=60)
                                                              AAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAA                                 >  SRR4302540.3587486/1‑94 (MQ=60)
                                                              AAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAA                                 >  SRR4302540.6141393/1‑94 (MQ=60)
                                                               AGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAA                                <  SRR4302540.7035062/94‑1 (MQ=60)
                                                               AGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAA                                >  SRR4302540.8760776/1‑94 (MQ=60)
                                                               AGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAA                                >  SRR4302540.2301706/1‑94 (MQ=60)
                                                                GTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAA                               >  SRR4302540.4295010/1‑94 (MQ=60)
                                                                GTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAA                               >  SRR4302540.345094/1‑94 (MQ=60)
                                                                 TTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAAC                              >  SRR4302540.1395931/1‑94 (MQ=60)
                                                                   TAAGAACTCACACCCCTATCAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCT                            <  SRR4302540.6055806/94‑1 (MQ=60)
                                                                   TAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCT                            >  SRR4302540.9392684/1‑94 (MQ=60)
                                                                   TAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCT                            <  SRR4302540.2127372/94‑1 (MQ=60)
                                                                    AAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTG                           >  SRR4302540.7519864/1‑94 (MQ=60)
                                                                    AAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTG                           <  SRR4302540.9270182/94‑1 (MQ=60)
                                                                    AAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTG                           >  SRR4302540.182452/1‑94 (MQ=60)
                                                                    AAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTG                           <  SRR4302540.5821751/94‑1 (MQ=60)
                                                                        ACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTC                       >  SRR4302540.7603677/1‑94 (MQ=60)
                                                                         CTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCG                      <  SRR4302540.2163889/94‑1 (MQ=60)
                                                                         CTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCG                      <  SRR4302540.356409/94‑1 (MQ=60)
                                                                         CTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCG                      <  SRR4302540.4206140/94‑1 (MQ=60)
                                                                         CTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCG                      >  SRR4302540.5514275/1‑94 (MQ=60)
                                                                           CACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGTCAATATGGGGCTGGAAAAACTGGCTCGTA                    >  SRR4302540.7851950/1‑94 (MQ=60)
                                                                            ACACCACTATGAATCTTGCCGAATTAAAGCATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTAT                   <  SRR4302540.8504799/94‑1 (MQ=60)
                                                                            ACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTAT                   <  SRR4302540.9225101/94‑1 (MQ=60)
                                                                            ACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTAT                   <  SRR4302540.9912071/94‑1 (MQ=60)
                                                                            ACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTAT                   <  SRR4302540.4000382/94‑1 (MQ=60)
                                                                            ACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTAT                   <  SRR4302540.7713954/94‑1 (MQ=60)
                                                                            ACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTAT                   <  SRR4302540.5020662/94‑1 (MQ=60)
                                                                            ACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTAT                   >  SRR4302540.6190706/1‑94 (MQ=60)
                                                                             CACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATG                  >  SRR4302540.692518/1‑94 (MQ=60)
                                                                              ACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGC                 <  SRR4302540.3951363/94‑1 (MQ=60)
                                                                               CCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCG                <  SRR4302540.1227055/94‑1 (MQ=60)
                                                                                CACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGT               <  SRR4302540.592837/94‑1 (MQ=60)
                                                                                 ACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTA              >  SRR4302540.6985884/1‑94 (MQ=60)
                                                                                  CTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAA             >  SRR4302540.8573790/1‑94 (MQ=60)
                                                                                  CTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAA             <  SRR4302540.4526108/94‑1 (MQ=60)
                                                                                    ATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGC           >  SRR4302540.3362794/1‑94 (MQ=60)
                                                                                    ATGAATCTTGCCGAATTAAAGAATACGCCGGTTACTGAGCTGATCTCTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGC           <  SRR4302540.9058577/94‑1 (MQ=60)
                                                                                     TGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCA          >  SRR4302540.7808154/1‑94 (MQ=60)
                                                                                     TGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCA          <  SRR4302540.7941454/94‑1 (MQ=60)
                                                                                      GAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAG         <  SRR4302540.2523296/94‑1 (MQ=60)
                                                                                      GAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAG         >  SRR4302540.1534298/1‑94 (MQ=60)
                                                                                      GAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAG         >  SRR4302540.1261292/1‑94 (MQ=60)
                                                                                      GAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAG         <  SRR4302540.4233823/94‑1 (MQ=60)
                                                                                      GAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAAACTGGCTCGTATGCGTAAGCAG         <  SRR4302540.2441475/94‑1 (MQ=60)
                                                                                       AATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGG        >  SRR4302540.10021101/1‑94 (MQ=60)
                                                                                       AATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGG        <  SRR4302540.8009803/94‑1 (MQ=60)
                                                                                        ATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTCTGCGTAAGCAGGA       >  SRR4302540.3949158/1‑94 (MQ=60)
                                                                                        ATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGA       >  SRR4302540.9170233/1‑94 (MQ=60)
                                                                                        ATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGA       <  SRR4302540.10027880/94‑1 (MQ=60)
                                                                                        ATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGA       <  SRR4302540.8165589/94‑1 (MQ=60)
                                                                                         TCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGAC      <  SRR4302540.8816380/94‑1 (MQ=60)
                                                                                          CTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACA     >  SRR4302540.6191346/1‑94 (MQ=60)
                                                                                          CTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACA     <  SRR4302540.2243750/94‑1 (MQ=60)
                                                                                          CTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACA     >  SRR4302540.106119/1‑94 (MQ=60)
                                                                                          CTTGCCGAATTAAAGAATAAGCCGGTTTCTGAGCTGATCACTCTCGGCGAACATATGGGGCTGGAAAACCTGGCTCGTGTCCGTAAGCAGGACT     >  SRR4302540.3129352/1‑94 (MQ=60)
                                                                                            TGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCGCTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATT   <  SRR4302540.5266561/94‑1 (MQ=60)
                                                                                            TGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATT   >  SRR4302540.2795121/1‑94 (MQ=60)
                                                                                            TGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATT   >  SRR4302540.9578174/1‑94 (MQ=60)
                                                                                            TGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATT   <  SRR4302540.7478730/94‑1 (MQ=60)
                                                                                             GCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTT  >  SRR4302540.1192031/1‑94 (MQ=60)
                                                                                             |                                                                                             
CCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTA  >  NC_000913/3963948‑3964134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑