Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	1624	250.6	12620048	98.0%	12367647	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,964,041	A→G	T4A (ACC→GCC)	rho →	transcription termination factor Rho

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,964,041	0	A	G	100.0%	855.7 / NA	263	T4A (ACC→GCC)	rho	transcription termination factor Rho
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (116/147); total (116/147)

Only 100 of 265 total aligned reads displayed.

TAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGA  >  NC_000913/3963951‑3964129

tAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGcc                                                                                        <  2:5121213/93‑1 (MQ=255)

tAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGcc                                                                                        >  1:1941708/1‑93 (MQ=255)

tAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGcc                                                                                        >  1:781306/1‑93 (MQ=255)

tAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGcc                                                                                        <  1:5645652/93‑1 (MQ=255)

 aaaCAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCg                                                                                       >  2:516538/1‑93 (MQ=255)

   aCAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGaa                                                                                     <  1:3053697/93‑1 (MQ=255)

   aCAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGaa                                                                                     <  1:992349/93‑1 (MQ=255)

      ggCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTa                                                                                  >  1:1140866/1‑93 (MQ=255)

      ggCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTa                                                                                  >  1:5454774/1‑93 (MQ=255)

       gCATGGATGACCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTaa                                                                                 <  2:661647/93‑1 (MQ=255)

       gCATGAATAATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTaa                                                                                 <  2:565622/93‑1 (MQ=255)

        cATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTaaa                                                                                <  1:5096168/93‑1 (MQ=255)

         aTGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAg                                                                               <  2:183753/93‑1 (MQ=255)

          tgcatgatCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGa                                                                              <  1:2878824/90‑1 (MQ=255)

          tGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGa                                                                              >  2:4117663/1‑93 (MQ=255)

          tGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGa                                                                              <  1:1372173/93‑1 (MQ=255)

          tGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGa                                                                              <  1:4706384/93‑1 (MQ=255)

          tGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGa                                                                              >  1:4416097/1‑93 (MQ=255)

          tGGATGACCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGa                                                                              <  1:1211263/93‑1 (MQ=255)

           ggATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGaa                                                                             <  1:3468064/93‑1 (MQ=255)

            gatgatCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAAt                                                                            >  2:2531754/1‑93 (MQ=255)

            gatgatCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAAt                                                                            >  2:5782421/1‑93 (MQ=255)

             atgatCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATa                                                                           <  1:2344408/93‑1 (MQ=255)

             atgatCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATa                                                                           <  2:1055441/93‑1 (MQ=255)

             atgatCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATa                                                                           <  1:50609/93‑1 (MQ=255)

             atgatCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATa                                                                           <  2:4441585/93‑1 (MQ=255)

             atgacCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATa                                                                           <  2:3280543/93‑1 (MQ=255)

                atCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGc                                                                        >  1:6058538/1‑93 (MQ=255)

                 tCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGcc                                                                       >  2:2716499/1‑93 (MQ=255)

                 tCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGcc                                                                       <  1:1620791/93‑1 (MQ=255)

                  ccTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCg                                                                      <  2:2908777/93‑1 (MQ=255)

                   cTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCgg                                                                     <  2:1480190/93‑1 (MQ=255)

                   cTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCgg                                                                     <  2:6286479/93‑1 (MQ=255)

                    tGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGt                                                                    >  1:2198138/1‑93 (MQ=255)

                    tGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGt                                                                    <  2:3664172/93‑1 (MQ=255)

                    tGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATT‑AAGAATACGCCGGtt                                                                   >  1:98791/1‑93 (MQ=255)

                       cATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTc                                                                 <  1:5407301/93‑1 (MQ=255)

                       cATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTc                                                                 >  1:4095925/1‑93 (MQ=255)

                        aTACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCt                                                                >  2:4907567/1‑93 (MQ=255)

                        aTACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCt                                                                <  2:3575668/93‑1 (MQ=255)

                        aTACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCt                                                                >  1:3898227/1‑93 (MQ=255)

                         tACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTg                                                               >  1:6305255/1‑93 (MQ=255)

                          aCCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGa                                                              >  1:4259512/1‑93 (MQ=255)

                           ccATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAg                                                             >  1:896168/1‑93 (MQ=255)

                            cATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGc                                                            <  1:2162898/93‑1 (MQ=255)

                            cATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGc                                                            <  2:2485781/93‑1 (MQ=255)

                             aTTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCt                                                           >  2:3840284/1‑93 (MQ=255)

                              ttCATAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTg                                                          >  1:666495/1‑93 (MQ=255)

                                cacaACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGAt                                                        >  2:3234169/1‑93 (MQ=255)

                                cacaACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGAt                                                        <  2:894585/93‑1 (MQ=255)

                                cacaACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGAt                                                        >  2:5333882/1‑93 (MQ=255)

                                  caacaTTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCa                                                      <  1:1244387/93‑1 (MQ=255)

                                   aacaTTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCAc                                                     <  2:4819617/93‑1 (MQ=255)

                                   aacaTTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCAc                                                     <  2:3438850/93‑1 (MQ=255)

                                       ttAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACtctc                                                 <  1:4798353/93‑1 (MQ=255)

                                       ttAAGTTCGAGAGTTACCCCAAGTTTAAGACCTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACtctc                                                 <  1:4385269/93‑1 (MQ=255)

                                        tAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCg                                                <  1:5529099/93‑1 (MQ=255)

                                        tAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCg                                                <  1:3956440/93‑1 (MQ=255)

                                         aaGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCgg                                               <  2:1198259/93‑1 (MQ=255)

                                           gTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCg                                             >  1:2721500/1‑93 (MQ=255)

                                           gTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCg                                             >  2:608840/1‑93 (MQ=255)

                                               gagaTTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGaaaa                                         >  2:3760460/1‑93 (MQ=255)

                                               gagaTTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGaaaa                                         <  2:417791/93‑1 (MQ=255)

                                               gagaTTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGaaaa                                         <  1:3629044/93‑1 (MQ=255)

                                                agaTTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAat                                        >  1:5127712/1‑93 (MQ=255)

                                                 gaTTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAata                                       <  2:1707028/93‑1 (MQ=255)

                                                   tttACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATg                                     <  2:4700991/93‑1 (MQ=255)

                                                   tttACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATg                                     <  1:5935274/93‑1 (MQ=255)

                                                    ttACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATgg                                    <  1:4510201/93‑1 (MQ=255)

                                                      aCCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATgggg                                  <  1:3812927/93‑1 (MQ=255)

                                                       ccccAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGc                                 <  2:5832464/93‑1 (MQ=255)

                                                          cAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTgg                              >  1:3252845/1‑93 (MQ=255)

                                                            aGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGaa                            <  2:4874840/93‑1 (MQ=255)

                                                             gTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGaaa                           >  1:3508456/1‑93 (MQ=255)

                                                             gTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGaaa                           >  1:58585/1‑93 (MQ=255)

                                                              tttAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGaaaa                          <  2:1889356/93‑1 (MQ=255)

                                                                tAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAAcc                        <  2:2849847/93‑1 (MQ=255)

                                                                tAAGAACTCACACCACTATGAATCTTGCCGAATAAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAAcc                        <  2:1964236/93‑1 (MQ=255)

                                                                 aaGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCt                       <  1:4026477/93‑1 (MQ=255)

                                                                    aaCTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGc                    <  1:4169113/93‑1 (MQ=255)

                                                                    aaCTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGc                    <  2:2645648/93‑1 (MQ=255)

                                                                            ccacTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGCAAATATGGGGCTGGAAAACCTGGCTCGTATGc            >  2:244016/1‑93 (MQ=255)

                                                                            ccacTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTAtcc            <  2:3973721/93‑3 (MQ=255)

                                                                            ccacTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGc            <  2:2144070/93‑1 (MQ=255)

                                                                              acTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGt          >  1:312915/1‑93 (MQ=255)

                                                                               cTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTa         >  2:1779787/1‑93 (MQ=255)

                                                                               cTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTa         <  2:1410072/93‑1 (MQ=255)

                                                                               cTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTa         >  1:1755656/1‑93 (MQ=255)

                                                                               cTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTa         >  1:3298727/1‑93 (MQ=255)

                                                                                tATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTaa        <  1:1422018/93‑1 (MQ=255)

                                                                                tATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTaa        <  2:5955198/93‑1 (MQ=255)

                                                                                tATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTaa        <  1:2664961/93‑1 (MQ=255)

                                                                                 aTGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAg       >  2:439267/1‑93 (MQ=255)

                                                                                 aTGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGGATTGGTAAg       >  2:1120692/1‑93 (MQ=255)

                                                                                  tGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGc      >  1:5622111/1‑93 (MQ=255)

                                                                                  tGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGc      <  2:4318392/93‑1 (MQ=255)

                                                                                  tGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGc      <  2:75870/93‑1 (MQ=255)

                                                                                  tGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGc      >  2:458505/1‑93 (MQ=255)

                                                                                   gAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCa     <  1:5225474/93‑1 (MQ=255)

                                                                                      tCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGGAAGCAGGa  <  2:6131461/93‑1 (MQ=255)

TAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGA  >  NC_000913/3963951‑3964129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTA  >  NC_000913/3963948‑3964134

CCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTG                                                                                               <  SRR4302533.5547441/94‑1 (MQ=60)

CCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTG                                                                                               >  SRR4302533.3098226/1‑94 (MQ=60)

CCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTG                                                                                               >  SRR4302533.682778/1‑94 (MQ=60)

CCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTG                                                                                               <  SRR4302533.4600459/94‑1 (MQ=60)

 CGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGC                                                                                              >  SRR4302533.156607/1‑94 (MQ=60)

  GTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCC                                                                                             >  SRR4302533.1953551/1‑94 (MQ=60)

  GTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCC                                                                                             >  SRR4302533.785907/1‑94 (MQ=60)

  GTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCC                                                                                             <  SRR4302533.6236327/94‑1 (MQ=60)

   TAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCG                                                                                            <  SRR4302533.5679784/94‑1 (MQ=60)

    AAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGA                                                                                           <  SRR4302533.4116688/94‑1 (MQ=60)

    AAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGA                                                                                           >  SRR4302533.615111/1‑94 (MQ=60)

    AAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGA                                                                                           <  SRR4302533.5362524/94‑1 (MQ=60)

     AACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAA                                                                                          >  SRR4302533.233823/1‑94 (MQ=60)

      ACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAAT                                                                                         <  SRR4302533.998151/94‑1 (MQ=60)

      ACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAAT                                                                                         <  SRR4302533.5196256/94‑1 (MQ=60)

      ACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAAT                                                                                         >  SRR4302533.5635200/1‑94 (MQ=60)

      ACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAAT                                                                                         <  SRR4302533.3072405/94‑1 (MQ=60)

       CAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATT                                                                                        >  SRR4302533.5769888/1‑94 (MQ=60)

        AGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTA                                                                                       >  SRR4302533.5487637/1‑94 (MQ=60)

        AGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTA                                                                                       >  SRR4302533.1147660/1‑94 (MQ=60)

        AGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTA                                                                                       >  SRR4302533.995069/1‑94 (MQ=60)

           CATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAG                                                                                    <  SRR4302533.5126961/94‑1 (MQ=60)

            ATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGA                                                                                   >  SRR4302533.4442764/1‑94 (MQ=60)

            ATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGA                                                                                   >  SRR4302533.3557227/1‑94 (MQ=60)

            ATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGA                                                                                   >  SRR4302533.2728439/1‑94 (MQ=60)

             TGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAA                                                                                  <  SRR4302533.4734908/94‑1 (MQ=60)

             TGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAA                                                                                  <  SRR4302533.1380425/94‑1 (MQ=60)

             TGGATGACCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAA                                                                                  <  SRR4302533.1218533/94‑1 (MQ=60)

             TGCATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAA                                                                                  <  SRR4302533.2896535/94‑1 (MQ=60)

              GGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAAT                                                                                 <  SRR4302533.3489472/94‑1 (MQ=60)

               GATGCTCCTGCCATACCATTCACAACATTTAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATA                                                                                <  SRR4302533.4216885/94‑1 (MQ=60)

               GATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATA                                                                                <  SRR4302533.3437445/94‑1 (MQ=60)

               GATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATA                                                                                <  SRR4302533.4031769/94‑1 (MQ=60)

                ATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATAC                                                                               <  SRR4302533.5664376/94‑1 (MQ=60)

                ATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATAC                                                                               <  SRR4302533.50955/94‑1 (MQ=60)

                ATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATAC                                                                               <  SRR4302533.2358917/94‑1 (MQ=60)

                ATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATAC                                                                               <  SRR4302533.1324582/94‑1 (MQ=60)

                  GATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGC                                                                             >  SRR4302533.6095218/1‑94 (MQ=60)

                  GATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGC                                                                             <  SRR4302533.452422/94‑1 (MQ=60)

                   ATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCC                                                                            >  SRR4302533.3498878/1‑94 (MQ=60)

                   ATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCC                                                                            <  SRR4302533.1534841/94‑1 (MQ=60)

                   ATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCC                                                                            >  SRR4302533.6086509/1‑94 (MQ=60)

                    TCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCG                                                                           <  SRR4302533.1630610/94‑1 (MQ=60)

                    TCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCG                                                                           <  SRR4302533.3876286/94‑1 (MQ=60)

                    TCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCG                                                                           <  SRR4302533.709383/94‑1 (MQ=60)

                     CCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGG                                                                          >  SRR4302533.5238497/1‑94 (MQ=60)

                      CTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGT                                                                         >  SRR4302533.2211680/1‑94 (MQ=60)

                      CTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGT                                                                         >  SRR4302533.4626753/1‑94 (MQ=60)

                      CTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATT‑AAGAATACGCCGGTT                                                                        >  SRR4302533.99403/1‑94 (MQ=60)

                        GCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTT                                                                       >  SRR4302533.3227484/1‑94 (MQ=60)

                        GCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTT                                                                       <  SRR4302533.4020385/94‑1 (MQ=60)

                         CCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTC                                                                      <  SRR4302533.5921044/94‑1 (MQ=60)

                         CCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTC                                                                      >  SRR4302533.4120859/1‑94 (MQ=60)

                          CATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCT                                                                     <  SRR4302533.1488204/94‑1 (MQ=60)

                          CATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCT                                                                     <  SRR4302533.3502441/94‑1 (MQ=60)

                          CATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCT                                                                     <  SRR4302533.5439795/94‑1 (MQ=60)

                          CATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCT                                                                     >  SRR4302533.3922010/1‑94 (MQ=60)

                           ATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTG                                                                    <  SRR4302533.3293402/94‑1 (MQ=60)

                           ATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTG                                                                    >  SRR4302533.1427354/1‑94 (MQ=60)

                           ATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTG                                                                    >  SRR4302533.3944665/1‑94 (MQ=60)

                           ATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTG                                                                    >  SRR4302533.5428447/1‑94 (MQ=60)

                           ATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTG                                                                    >  SRR4302533.2406780/1‑94 (MQ=60)

                           ATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTG                                                                    >  SRR4302533.6342985/1‑94 (MQ=60)

                           ATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTG                                                                    >  SRR4302533.636324/1‑94 (MQ=60)

                            TACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGA                                                                   >  SRR4302533.4285182/1‑94 (MQ=60)

                             ACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAG                                                                  >  SRR4302533.901431/1‑94 (MQ=60)

                              CCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGC                                                                 >  SRR4302533.949022/1‑94 (MQ=60)

                               CATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCT                                                                >  SRR4302533.2016069/1‑94 (MQ=60)

                               CATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCT                                                                <  SRR4302533.2176207/94‑1 (MQ=60)

                                ATTCATAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTG                                                               >  SRR4302533.670433/1‑94 (MQ=60)

                                ATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTG                                                               >  SRR4302533.3026006/1‑94 (MQ=60)

                                   CACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATC                                                            <  SRR4302533.5100737/94‑1 (MQ=60)

                                     CAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCAC                                                          <  SRR4302533.1251818/94‑1 (MQ=60)

                                     CAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCAC                                                          >  SRR4302533.125074/1‑94 (MQ=60)

                                      AACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACT                                                         <  SRR4302533.260333/94‑1 (MQ=60)

                                       ACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTC                                                        >  SRR4302533.1823368/1‑94 (MQ=60)

                                        CATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCT                                                       <  SRR4302533.76681/94‑1 (MQ=60)

                                        CATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCT                                                       <  SRR4302533.2140457/94‑1 (MQ=60)

                                         ATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTC                                                      >  SRR4302533.1793142/1‑94 (MQ=60)

                                          TTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCG                                                     <  SRR4302533.2207219/94‑1 (MQ=60)

                                          TTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCG                                                     <  SRR4302533.4827416/94‑1 (MQ=60)

                                          TTAAGTTCGAGAGTTACCCCAAGTTTAAGACCTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCG                                                     <  SRR4302533.4411747/94‑1 (MQ=60)

                                           TAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGG                                                    <  SRR4302533.5562480/94‑1 (MQ=60)

                                           TAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGG                                                    <  SRR4302533.3980556/94‑1 (MQ=60)

                                             AGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCG                                                  >  SRR4302533.2738202/1‑94 (MQ=60)

                                                TCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAA                                               >  SRR4302533.2957669/1‑94 (MQ=60)

                                                  GAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAAT                                             <  SRR4302533.4439785/94‑1 (MQ=60)

                                                  GAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAAT                                             <  SRR4302533.3651374/94‑1 (MQ=60)

                                                  GAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAAT                                             >  SRR4302533.5158690/1‑94 (MQ=60)

                                                   AGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATA                                            >  SRR4302533.2345565/1‑94 (MQ=60)

                                                   AGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATA                                            >  SRR4302533.2780784/1‑94 (MQ=60)

                                                   AGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATA                                            <  SRR4302533.838957/94‑1 (MQ=60)

                                                      TTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAACTATGG                                         >  SRR4302533.5140947/1‑94 (MQ=60)

                                                      TTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGG                                         <  SRR4302533.5971156/94‑1 (MQ=60)

                                                       TTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGG                                        <  SRR4302533.4537515/94‑1 (MQ=60)

                                                         ACCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGC                                      <  SRR4302533.3836244/94‑1 (MQ=60)

                                                          CCCCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCT                                     >  SRR4302533.4541730/1‑94 (MQ=60)

                                                            CCAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGG                                   >  SRR4302533.3272904/1‑94 (MQ=60)

                                                             CAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGA                                  >  SRR4302533.3850134/1‑94 (MQ=60)

                                                             CAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGA                                  >  SRR4302533.5049446/1‑94 (MQ=60)

                                                             CAAGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGA                                  <  SRR4302533.1735925/94‑1 (MQ=60)

                                                               AGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAA                                >  SRR4302533.58971/1‑94 (MQ=60)

                                                               AGTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAA                                >  SRR4302533.3530088/1‑94 (MQ=60)

                                                                GTTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAA                               <  SRR4302533.475366/94‑1 (MQ=60)

                                                                 TTTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAAC                              >  SRR4302533.4883722/1‑94 (MQ=60)

                                                                  TTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACC                             <  SRR4302533.580374/94‑1 (MQ=60)

                                                                  TTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACC                             <  SRR4302533.1237591/94‑1 (MQ=60)

                                                                  TTAAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACC                             >  SRR4302533.6065454/1‑94 (MQ=60)

                                                                    AAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTG                           >  SRR4302533.1177166/1‑94 (MQ=60)

                                                                    AAGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTG                           <  SRR4302533.4050987/94‑1 (MQ=60)

                                                                     AGAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGG                          <  SRR4302533.5473143/94‑1 (MQ=60)

                                                                      GAACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGC                         <  SRR4302533.6330492/94‑1 (MQ=60)

                                                                       AACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCT                        <  SRR4302533.4194378/94‑1 (MQ=60)

                                                                       AACTCACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCT                        <  SRR4302533.99425/94‑1 (MQ=60)

                                                                            ACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTAT                   <  SRR4302533.5531668/94‑1 (MQ=60)

                                                                            ACACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTAT                   >  SRR4302533.3747047/1‑94 (MQ=60)

                                                                             CACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGGTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATG                  >  SRR4302533.4113431/1‑94 (MQ=60)

                                                                             CACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATG                  <  SRR4302533.3277905/94‑1 (MQ=60)

                                                                              ACCACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGC                 >  SRR4302533.4159026/1‑94 (MQ=60)

                                                                                CACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGT               <  SRR4302533.832060/94‑1 (MQ=60)

                                                                                CACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGT               >  SRR4302533.314825/1‑94 (MQ=60)

                                                                                 ACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTA              <  SRR4302533.5114166/94‑1 (MQ=60)

                                                                                 ACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTA              >  SRR4302533.1766352/1‑94 (MQ=60)

                                                                                 ACTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTA              >  SRR4302533.3319082/1‑94 (MQ=60)

                                                                                  CTATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAA             <  SRR4302533.1399606/94‑1 (MQ=60)

                                                                                   TATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAG            <  SRR4302533.2681315/94‑1 (MQ=60)

                                                                                   TATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAG            <  SRR4302533.3104054/94‑1 (MQ=60)

                                                                                   TATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAG            >  SRR4302533.4361870/1‑94 (MQ=60)

                                                                                   TATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAG            <  SRR4302533.1430616/94‑1 (MQ=60)

                                                                                   TATGAATCTTGCCGAATTAAAGAATACGCCGGGTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAG            >  SRR4302533.3151087/1‑94 (MQ=60)

                                                                                    ATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGC           >  SRR4302533.5496699/1‑94 (MQ=60)

                                                                                    ATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGC           >  SRR4302533.5656093/1‑94 (MQ=60)

                                                                                    ATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGC           <  SRR4302533.4248258/94‑1 (MQ=60)

                                                                                    ATGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGC           <  SRR4302533.2877345/94‑1 (MQ=60)

                                                                                     TGAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCCTATGCGTAAGCA          >  SRR4302533.3595812/1‑94 (MQ=60)

                                                                                      GAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAG         <  SRR4302533.5256919/94‑1 (MQ=60)

                                                                                      GAATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAG         <  SRR4302533.4294136/94‑1 (MQ=60)

                                                                                        ATCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGA       >  SRR4302533.5577548/1‑94 (MQ=60)

                                                                                         TCTTGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGAC      <  SRR4302533.4534559/94‑1 (MQ=60)

                                                                                            TGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATT   >  SRR4302533.5724699/1‑94 (MQ=60)

                                                                                            TGCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATT   >  SRR4302533.134597/1‑94 (MQ=60)

                                                                                             GCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTA  <  SRR4302533.5880005/94‑1 (MQ=60)

                                                                                             GCCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTA  >  SRR4302533.4953571/1‑94 (MQ=60)

CCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAAATATGGGGCTGGAAAACCTGGCTCGTATGCGTAAGCAGGACATTA  >  NC_000913/3963948‑3964134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`