Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,969,103 2 bp→GC coding (232‑233/1263 nt) wecC → UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,969,1030CG99.8% 1346.0 / NA 421R78A (CGC→GCC) wecCUDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (166/254);  total (166/255)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009133,969,1040GC99.8% 1314.5 / NA 409R78A (CGC→GCC) wecCUDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (159/249);  minor base A (1/0);  total (160/249)
Fisher's exact test for biased strand distribution p-value = 3.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 428 total aligned reads displayed.

ATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGC  >  NC_000913/3969016‑3969190
                                                                                       ||                                                                                      
atCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctgg                                                                                    >  2:532225/1‑93 (MQ=255)
  cgtcgAACCTGATTTGGGGAGTGTAGAAAAAACTGCCGTAGAAGGCGTTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggct                                                                                  <  2:9192025/93‑1 (MQ=255)
  cgtcgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggct                                                                                  >  1:5160013/1‑93 (MQ=255)
  cgtcgAACCTGATTGGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggct                                                                                  <  2:6166064/93‑1 (MQ=255)
   gtcgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggctg                                                                                 <  1:876322/93‑1 (MQ=255)
    tcgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGa                                                                                >  2:9728135/1‑93 (MQ=255)
    tcgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGa                                                                                >  1:9950609/1‑93 (MQ=255)
    ccgAACCTCATTTGGCAATTGTAGTAAAACCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGa                                                                                <  2:7639239/92‑1 (MQ=255)
     cgAACCTGATTTGGCGCGTGTAGTAAAAACTGCCGTAGCAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGgatgat                                                                               >  2:6733843/1‑93 (MQ=255)
     cgAACCTGATTGGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAt                                                                               <  1:4355412/93‑1 (MQ=255)
     cgAACATAATTGGGCGAGTGTAAAAAAAACTGCCGTAGAGGGGGTTTTTTTACGAGCGATCACGACGCCAGTTGAAGCGGATGCCTGGCTGAt                                                                               <  2:5441797/93‑1 (MQ=255)
      gacccTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAtt                                                                              <  2:2790228/90‑1 (MQ=255)
      gAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAtt                                                                              <  1:698209/93‑1 (MQ=255)
      gAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAtt                                                                              <  1:3309993/93‑1 (MQ=255)
      gAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAtt                                                                              <  2:5255375/93‑1 (MQ=255)
       aaCCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTg                                                                             >  2:2964804/1‑93 (MQ=255)
        gctagATTGGGCGAGTGAAAAAAAAACTGCAGTAGAGGGCGTTTTTTTACGGGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc                                                                            <  2:8830902/89‑1 (MQ=255)
        aCCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc                                                                            >  1:8146570/1‑93 (MQ=255)
        aCCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc                                                                            >  2:9362972/1‑93 (MQ=255)
          cttaTTTGGCGAGTGTAGAAAAAACTGCGGAAAAAGGCGGTTTTTAGCGAGCGCCTACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTg                                                                          <  1:5702427/90‑1 (MQ=255)
          cTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTg                                                                          >  1:312614/1‑93 (MQ=255)
          cTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTg                                                                          >  2:319361/1‑93 (MQ=255)
           tGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGt                                                                         <  1:3039271/93‑1 (MQ=255)
           tGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGt                                                                         >  1:4698949/1‑93 (MQ=255)
           tGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATAGCTGt                                                                         >  1:9398314/1‑93 (MQ=255)
            gATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTa                                                                        <  1:2665479/93‑1 (MQ=255)
                tGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTATGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCa                                                                    <  1:640298/93‑1 (MQ=255)
                tGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCa                                                                    <  1:4878044/93‑1 (MQ=255)
                 ggCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAc                                                                   <  1:7922379/93‑1 (MQ=255)
                   cGAGTGTAGTAAAAACTACCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGc                                                                 >  2:636185/1‑93 (MQ=255)
                    gAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGcc                                                                >  1:5568769/1‑93 (MQ=255)
                    gAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGcc                                                                >  2:7380067/1‑93 (MQ=255)
                    gAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGcc                                                                >  1:10002353/1‑93 (MQ=255)
                       tgtAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGtt                                                             <  1:4387557/93‑1 (MQ=255)
                       tgtAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGtt                                                             >  1:2463761/1‑93 (MQ=255)
                        gtagtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGttt                                                            >  2:4552242/1‑93 (MQ=255)
                        gtagtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGACTGGCTGATTGCTGTACCCACGCCGttt                                                            >  2:1302964/1‑93 (MQ=255)
                          agtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCTAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTaa                                                          <  2:8241903/93‑1 (MQ=255)
                             aaaaaCTGCAGTAGAAGGGGGTTTTTTACGAGAGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAggg                                                       <  1:1911166/93‑1 (MQ=255)
                              aaaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGc                                                      <  2:5108448/93‑1 (MQ=255)
                              aaaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGc                                                      >  2:5916408/1‑93 (MQ=255)
                              aaaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGc                                                      <  1:585555/93‑1 (MQ=255)
                                accTGCCGTAAAGGGAGTTTTTTTAAGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGa                                                    <  2:9233809/91‑1 (MQ=255)
                                aaCTGCCGTAGAAGACGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGa                                                    <  2:8300140/93‑1 (MQ=255)
                                 aCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGAt                                                   >  2:4723173/1‑93 (MQ=255)
                                 aCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGAt                                                   <  1:908038/93‑1 (MQ=255)
                                  cTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATc                                                  >  2:878285/1‑93 (MQ=255)
                                  cTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATc                                                  >  2:6452544/1‑93 (MQ=255)
                                   ggCCGTAGAGGGCGGTTTTTAACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCa                                                 <  2:2082274/92‑1 (MQ=255)
                                     ccGTAGAAGGCGTTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATg                                               <  1:2059023/93‑1 (MQ=255)
                                      cGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGa                                              <  1:9198302/93‑1 (MQ=255)
                                       gTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAg                                             >  1:5489241/1‑93 (MQ=255)
                                       gTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAg                                             >  1:5011379/1‑93 (MQ=255)
                                        tAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGc                                            >  1:2219781/1‑93 (MQ=255)
                                          gAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCa                                          >  1:1200193/1‑93 (MQ=255)
                                           aaGGTGTTTTTTGACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAg                                         <  1:3922212/93‑1 (MQ=255)
                                           aaGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAg                                         <  2:1567852/93‑1 (MQ=255)
                                            aGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGa                                        >  2:7971349/1‑93 (MQ=255)
                                              ggttttttttACGGGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata                                      <  2:1908947/89‑1 (MQ=255)
                                              gctgTTTTTTACAAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTCTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata                                      <  2:1384624/90‑1 (MQ=255)
                                              gCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata                                      <  2:1188250/93‑1 (MQ=255)
                                              gCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata                                      >  1:8614860/1‑93 (MQ=255)
                                              gCGGTTTTTCACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata                                      <  2:8523993/93‑1 (MQ=255)
                                               gggTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGatat                                     <  1:4037582/92‑1 (MQ=255)
                                               cGGTTTTTTACGAGGGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGatat                                     <  2:8054617/93‑1 (MQ=255)
                                               cGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGACCCAGatat                                     >  1:8667241/1‑93 (MQ=255)
                                                ggTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATg                                    <  1:9709668/93‑1 (MQ=255)
                                                 gTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGa                                   >  2:3928705/1‑93 (MQ=255)
                                                  gtttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAc                                  <  1:1101658/92‑1 (MQ=255)
                                                   tttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAcc                                 <  2:5733601/93‑1 (MQ=255)
                                                    ttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCt                                <  1:6814533/93‑1 (MQ=255)
                                                    ttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCt                                >  1:7259627/1‑93 (MQ=255)
                                                     tttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTa                               <  2:6081216/93‑1 (MQ=255)
                                                          gagcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTg                          <  1:7706437/93‑1 (MQ=255)
                                                            gcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGaa                        <  2:2452162/93‑1 (MQ=255)
                                                             cgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAt                       >  1:2375134/1‑93 (MQ=255)
                                                             cgaccACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAt                       <  1:7149899/93‑1 (MQ=255)
                                                              gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATc                      <  2:3531465/93‑1 (MQ=255)
                                                              gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATc                      <  2:7577180/93‑1 (MQ=255)
                                                                  acgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgct                  <  2:4891109/93‑1 (MQ=255)
                                                                  acgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCCGTACCCACGCCGTTTAATGGCGATCATGAGCCATATCTCACCTACGTTTAATCGgcc                  >  2:8463395/1‑92 (MQ=255)
                                                                   cgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctg                 >  1:4217194/1‑93 (MQ=255)
                                                                   cgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctg                 <  1:672002/93‑1 (MQ=255)
                                                                    gacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctgc                >  1:7447354/1‑93 (MQ=255)
                                                                     acgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctgct               >  1:6845815/1‑93 (MQ=255)
                                                                     acgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctgct               <  1:6102902/93‑1 (MQ=255)
                                                                       gCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcg             >  1:9781385/1‑93 (MQ=255)
                                                                       gCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcg             <  1:6943015/93‑1 (MQ=255)
                                                                        ccAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcgc            <  1:9647541/93‑1 (MQ=255)
                                                                        ccAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcgc            <  1:846305/93‑1 (MQ=255)
                                                                        ccAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcgc            <  1:1659507/93‑1 (MQ=255)
                                                                          aGTTGAGGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcgctc          >  2:6988895/1‑93 (MQ=255)
                                                                           gTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTcc         >  2:4275161/1‑93 (MQ=255)
                                                                           gTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTcc         >  2:951532/1‑93 (MQ=255)
                                                                            ttGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCa        <  1:8945866/93‑1 (MQ=255)
                                                                             tGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCAt       <  1:1579749/93‑1 (MQ=255)
                                                                              gAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCAtt      <  2:4077031/93‑1 (MQ=255)
                                                                               aaGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTg     <  1:2715605/93‑1 (MQ=255)
                                                                               aaGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTg     <  1:1364712/93‑1 (MQ=255)
                                                                                 gCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTgcg   <  2:9007401/93‑1 (MQ=255)
                                                                                       ||                                                                                      
ATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGC  >  NC_000913/3969016‑3969190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 240 total aligned reads displayed.
ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCA  >  NC_000913/3969010‑3969192
                                                                                                 |                                                                                         
ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATG                                                                                           >  SRR4302540.2944105/1‑94 (MQ=60)
ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATG                                                                                           <  SRR4302540.981614/94‑1 (MQ=60)
ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATG                                                                                           >  SRR4302540.9116776/1‑94 (MQ=60)
     TATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGG                                                                                      >  SRR4302540.4065178/1‑94 (MQ=60)
       TCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCT                                                                                    >  SRR4302540.5216101/1‑94 (MQ=60)
        CGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTG                                                                                   <  SRR4302540.8730357/94‑1 (MQ=60)
        CGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTG                                                                                   >  SRR4302540.9839079/1‑94 (MQ=60)
         GTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGA                                                                                  >  SRR4302540.10066779/1‑94 (MQ=60)
         GTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGA                                                                                  <  SRR4302540.885625/94‑1 (MQ=60)
          TCGAACGTGATTGGGGGATTGTAGAAAAAACTGCCGAAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGAT                                                                                 <  SRR4302540.2083925/94‑1 (MQ=60)
          TCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGAT                                                                                 >  SRR4302540.1149213/1‑94 (MQ=60)
          TCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGAT                                                                                 >  SRR4302540.3155049/1‑94 (MQ=60)
          TCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGAT                                                                                 <  SRR4302540.850833/94‑1 (MQ=60)
           CGAACCTGATTGGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATT                                                                                <  SRR4302540.4402785/94‑1 (MQ=60)
            GAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTG                                                                               <  SRR4302540.3346255/94‑1 (MQ=60)
            GAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTG                                                                               <  SRR4302540.705698/94‑1 (MQ=60)
            GAACCTGATTTGGCGAGTGTAGTAAAAACAGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTG                                                                               >  SRR4302540.2375974/1‑94 (MQ=60)
             AACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑CCGACGCCAGTTGAAGCGGATGCCTGGCTGATTGT                                                                              >  SRR4302540.2114553/1‑94 (MQ=60)
             AACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGC                                                                              >  SRR4302540.3074142/1‑94 (MQ=60)
             AACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGC                                                                              >  SRR4302540.8241143/1‑94 (MQ=60)
             AACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGC                                                                              <  SRR4302540.6798095/94‑1 (MQ=60)
              ACTTGATTGGGCGAGTGTAAAAAAAACGCCGGTAGAAGGCGTTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCT                                                                             <  SRR4302540.7209939/94‑1 (MQ=50)
              ACCTGATTGGGCGAGTGTAGTAAAAGCTGCCGTAGAAGGTGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCT                                                                             <  SRR4302540.5584878/94‑1 (MQ=60)
               CCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTG                                                                            >  SRR4302540.9881541/1‑94 (MQ=60)
               CCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTG                                                                            >  SRR4302540.1620671/1‑94 (MQ=60)
               CCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTG                                                                            >  SRR4302540.315726/1‑94 (MQ=60)
                CTTATTTGGCGAGTGTAGAAAAAACTGCGGAAAAAGGCGGTTTTT‑‑‑‑AGCGAGCGCCTACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGT                                                                           <  SRR4302540.5765214/94‑1 (MQ=28)
                CTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGT                                                                           <  SRR4302540.6742982/94‑1 (MQ=60)
                CTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGT                                                                           <  SRR4302540.2809669/94‑1 (MQ=60)
                CTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGT                                                                           <  SRR4302540.8734907/94‑1 (MQ=60)
                CTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATAGCTGT                                                                           >  SRR4302540.9510132/1‑94 (MQ=60)
                CTGATTGGGGGAGTGTAGAAAAAGCGGCGGTAGAAGGTGTTTTTTGACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGT                                                                           <  SRR4302540.7399395/94‑1 (MQ=34)
                 TGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTA                                                                          <  SRR4302540.1553999/94‑1 (MQ=60)
                  GATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAC                                                                         >  SRR4302540.159581/1‑94 (MQ=60)
                  GATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAC                                                                         <  SRR4302540.2694377/94‑1 (MQ=60)
                   tttttGCGAGTGTAGGAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACC                                                                        <  SRR4302540.8642137/89‑1 (MQ=60)
                   tttggggggtgtaaaaaaaaacggcggaAGAAGGCTGTTTTTTACGGGCGGGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACC                                                                        <  SRR4302540.1923025/66‑1 (MQ=50)
                   ATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACC                                                                        <  SRR4302540.8642139/94‑1 (MQ=60)
                   ATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACC                                                                        <  SRR4302540.5064414/94‑1 (MQ=60)
                      TGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTATGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAC                                                                     <  SRR4302540.647120/94‑1 (MQ=60)
                      TGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAC                                                                     <  SRR4302540.4930413/94‑1 (MQ=60)
                      TGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAC                                                                     <  SRR4302540.7104997/94‑1 (MQ=60)
                      TGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAC                                                                     <  SRR4302540.1684575/94‑1 (MQ=60)
                       GGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACG                                                                    <  SRR4302540.6606780/94‑1 (MQ=60)
                       GGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACG                                                                    <  SRR4302540.6407419/94‑1 (MQ=60)
                       GGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACG                                                                    <  SRR4302540.8631281/94‑1 (MQ=60)
                       GGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACG                                                                    >  SRR4302540.126273/1‑94 (MQ=60)
                       GGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACG                                                                    <  SRR4302540.8028076/94‑1 (MQ=60)
                       GGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACG                                                                    <  SRR4302540.8014728/94‑1 (MQ=60)
                        GCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGC                                                                   <  SRR4302540.5304411/94‑1 (MQ=60)
                         CGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCC                                                                  >  SRR4302540.433257/1‑94 (MQ=60)
                         CGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCC                                                                  >  SRR4302540.5037335/1‑94 (MQ=60)
                         CGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCC                                                                  >  SRR4302540.5630090/1‑94 (MQ=60)
                         CGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCC                                                                  >  SRR4302540.10118869/1‑94 (MQ=60)
                         CGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCC                                                                  >  SRR4302540.10118870/1‑94 (MQ=60)
                          GAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAGGCCG                                                                 >  SRR4302540.2177930/1‑94 (MQ=60)
                          GAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCG                                                                 >  SRR4302540.4471181/1‑94 (MQ=60)
                          GAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCG                                                                 >  SRR4302540.9109841/1‑94 (MQ=60)
                           AGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGT                                                                <  SRR4302540.8890251/94‑1 (MQ=60)
                            GTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTaagcgctagatcggaag                                                               >  SRR4302540.7004611/1‑77 (MQ=60)
                            GTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTT                                                               >  SRR4302540.5467106/1‑94 (MQ=60)
                            GTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTT                                                               >  SRR4302540.2490279/1‑94 (MQ=60)
                            GTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTT                                                               >  SRR4302540.7458341/1‑94 (MQ=60)
                             TGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTT                                                              >  SRR4302540.2500776/1‑94 (MQ=60)
                             TGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTT                                                              <  SRR4302540.4435183/94‑1 (MQ=60)
                             TGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTT                                                              >  SRR4302540.1270977/1‑94 (MQ=60)
                             TGTAGAAAAAACGGCCGTAGAGGGCGTTTTTTGACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTGCCCACGCCGTTT                                                              <  SRR4302540.7730433/94‑1 (MQ=60)
                             TGTAAAAAAAACTGCGGAAGAGGGCGTTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTT                                                              <  SRR4302540.7507575/94‑1 (MQ=60)
                              GTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTA                                                             >  SRR4302540.5576817/1‑94 (MQ=60)
                              GTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTA                                                             <  SRR4302540.2476903/94‑1 (MQ=60)
                              GTAGTAAAAACTGCCGTACAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTA                                                             >  SRR4302540.813381/1‑94 (MQ=60)
                               TAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAA                                                            <  SRR4302540.4826618/94‑1 (MQ=60)
                                AGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAG                                                           >  SRR4302540.876516/1‑94 (MQ=60)
                                AGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAG                                                           >  SRR4302540.1114813/1‑94 (MQ=60)
                                 GTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGG                                                          >  SRR4302540.5645339/1‑94 (MQ=60)
                                 GTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGG                                                          >  SRR4302540.5398503/1‑94 (MQ=60)
                                 GTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGG                                                          >  SRR4302540.9772315/1‑94 (MQ=60)
                                 GTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGG                                                          <  SRR4302540.9816473/94‑1 (MQ=60)
                                  TAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGG                                                         <  SRR4302540.8751485/94‑1 (MQ=60)
                                  AAAAAACTGCCGTAGAGGGCGGTTTTTTACGAGCGACC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGG                                                         <  SRR4302540.3969481/94‑1 (MQ=60)
                                   AAAATCCGCCGTAGAAAGGGTTTTTTGACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGC                                                        <  SRR4302540.2415717/94‑1 (MQ=60)
                                   AAAAACTGCAGTAGAAGGGGGTTTTTTACGAGAGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGC                                                        <  SRR4302540.1932046/94‑1 (MQ=60)
                                    AAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCG                                                       <  SRR4302540.10080967/94‑1 (MQ=60)
                                      AACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGAT                                                     <  SRR4302540.2841571/94‑1 (MQ=60)
                                      AACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGAT                                                     <  SRR4302540.1435747/94‑1 (MQ=60)
                                       ACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATC                                                    <  SRR4302540.368159/94‑1 (MQ=60)
                                       ACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATC                                                    >  SRR4302540.603133/1‑94 (MQ=60)
                                        CTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCA                                                   <  SRR4302540.2707581/94‑1 (MQ=60)
                                         TGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCAT                                                  >  SRR4302540.4160915/1‑94 (MQ=60)
                                         TGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCAT                                                  <  SRR4302540.1752638/94‑1 (MQ=60)
                                         TGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCAT                                                  <  SRR4302540.2290161/94‑1 (MQ=60)
                                         CGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCAT                                                  <  SRR4302540.1596431/94‑1 (MQ=60)
                                           CCGTAGAAGGCGTTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGA                                                <  SRR4302540.2081187/94‑1 (MQ=60)
                                           CCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGA                                                >  SRR4302540.323051/1‑94 (MQ=60)
                                           CCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGA                                                <  SRR4302540.863420/94‑1 (MQ=60)
                                            CGTAGAGGGCTTTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAG                                               <  SRR4302540.5530865/94‑1 (MQ=60)
                                            CGTAGAAGGCGTTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAG                                               <  SRR4302540.9308639/94‑1 (MQ=60)
                                            CGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAG                                               >  SRR4302540.2035131/1‑94 (MQ=60)
                                            CGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAG                                               >  SRR4302540.5065640/1‑94 (MQ=60)
                                            CGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAG                                               <  SRR4302540.9308640/94‑1 (MQ=60)
                                            CGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAG                                               <  SRR4302540.2670517/94‑1 (MQ=60)
                                            CGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAG                                               >  SRR4302540.4777426/1‑94 (MQ=60)
                                             GTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGC                                              >  SRR4302540.9294612/1‑94 (MQ=60)
                                             GTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGC                                              <  SRR4302540.4413643/94‑1 (MQ=60)
                                               AGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCA                                            <  SRR4302540.7859765/94‑1 (MQ=60)
                                               AGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCA                                            >  SRR4302540.1212537/1‑94 (MQ=60)
                                               AGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCA                                            <  SRR4302540.7083218/94‑1 (MQ=60)
                                                GAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAG                                           <  SRR4302540.7054771/94‑1 (MQ=60)
                                                GAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAG                                           <  SRR4302540.7057014/94‑1 (MQ=60)
                                                GAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAG                                           <  SRR4302540.9815439/94‑1 (MQ=60)
                                                 AAGGTGTTTTTTGACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGA                                          <  SRR4302540.3964662/94‑1 (MQ=60)
                                                 AAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGA                                          >  SRR4302540.2411763/1‑94 (MQ=60)
                                                  agggtgtttttgaggcGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGACGCCTGGCTGATTGCTGTCCCCACGCCGTTTAAGGGCGATCATGAGCCAGAT                                         <  SRR4302540.2266001/78‑1 (MQ=60)
                                                  AGGGGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGAT                                         <  SRR4302540.3904851/94‑1 (MQ=60)
                                                  AGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGAT                                         <  SRR4302540.421316/94‑1 (MQ=60)
                                                  AGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGAT                                         <  SRR4302540.6914754/94‑1 (MQ=60)
                                                  AGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGAT                                         <  SRR4302540.9425008/94‑1 (MQ=60)
                                                  AGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAACCACGCCGGTTAAGGGCGATCATGAGACAGAT                                         >  SRR4302540.5080842/1‑94 (MQ=60)
                                                   GGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATA                                        >  SRR4302540.7816153/1‑94 (MQ=60)
                                                   GGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCATTTAAGGGCGATCATGAGCCAGATA                                        >  SRR4302540.4429755/1‑94 (MQ=60)
                                                    GCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATAT                                       <  SRR4302540.690916/94‑1 (MQ=60)
                                                    GCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATAT                                       <  SRR4302540.8103436/94‑1 (MQ=60)
                                                    GCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGACCCAGATAT                                       >  SRR4302540.8767920/1‑94 (MQ=60)
                                                    GCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCGGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATAT                                       <  SRR4302540.9290538/94‑1 (MQ=60)
                                                     gttTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATG                                      <  SRR4302540.7314432/91‑1 (MQ=60)
                                                     gaGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATG                                      <  SRR4302540.6806692/92‑1 (MQ=60)
                                                     GGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATG                                      <  SRR4302540.4081112/94‑1 (MQ=60)
                                                     CGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATG                                      <  SRR4302540.8845066/94‑1 (MQ=60)
                                                      GGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGA                                     >  SRR4302540.5613702/1‑94 (MQ=60)
                                                       GTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAC                                    <  SRR4302540.3164638/94‑1 (MQ=60)
                                                        TTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACC                                   <  SRR4302540.4807172/94‑1 (MQ=60)
                                                        GTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACC                                   <  SRR4302540.1113008/94‑1 (MQ=60)
                                                         TTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCT                                  >  SRR4302540.7340631/1‑94 (MQ=60)
                                                         TTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCT                                  <  SRR4302540.4812446/94‑1 (MQ=60)
                                                          TTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTA                                 <  SRR4302540.6889959/94‑1 (MQ=60)
                                                           TTGACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAC                                <  SRR4302540.165000/94‑1 (MQ=60)
                                                           TATACGGGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAC                                <  SRR4302540.7324077/94‑1 (MQ=60)
                                                            TTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACG                               <  SRR4302540.83525/94‑1 (MQ=60)
                                                            TTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACG                               <  SRR4302540.5863391/94‑1 (MQ=60)
                                                            TTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACG                               <  SRR4302540.4710889/94‑1 (MQ=60)
                                                            TTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACG                               <  SRR4302540.9348902/94‑1 (MQ=60)
                                                               CGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTG                            <  SRR4302540.6020159/94‑1 (MQ=60)
                                                               CGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTG                            <  SRR4302540.6020158/94‑1 (MQ=60)
                                                                GAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGA                           <  SRR4302540.7794763/94‑1 (MQ=60)
                                                                   CGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATC                        <  SRR4302540.571706/94‑1 (MQ=60)
                                                                   CGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATC                        >  SRR4302540.8013350/1‑94 (MQ=60)
                                                                   CGACC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATC                        <  SRR4302540.7229577/94‑1 (MQ=60)
                                                                    GAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCG                       <  SRR4302540.2101780/94‑1 (MQ=60)
                                                                    GAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCG                       >  SRR4302540.5091403/1‑94 (MQ=60)
                                                                     AGC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGG                      <  SRR4302540.3271021/94‑1 (MQ=60)
                                                                      GC‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGC                     >  SRR4302540.1100128/1‑94 (MQ=60)
                                                                       C‑‑‑‑ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCT                    <  SRR4302540.7594977/94‑1 (MQ=60)
                                                                            ACGACGCCAGTTGAAGCGGATGCCTGGCTGCTTGCTGTACCCACGCCGTTTAACGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTG                   >  SRR4302540.5838131/1‑94 (MQ=60)
                                                                            ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTG                   >  SRR4302540.4263104/1‑94 (MQ=60)
                                                                            ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTG                   <  SRR4302540.9585586/94‑1 (MQ=60)
                                                                            ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTG                   >  SRR4302540.5838132/1‑94 (MQ=60)
                                                                             CGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGC                  <  SRR4302540.679204/94‑1 (MQ=60)
                                                                             CGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGC                  >  SRR4302540.7530341/1‑94 (MQ=60)
                                                                             CGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGC                  <  SRR4302540.6317272/94‑1 (MQ=60)
                                                                             CGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGC                  <  SRR4302540.2610151/94‑1 (MQ=60)
                                                                              GACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCT                 >  SRR4302540.6109895/1‑94 (MQ=60)
                                                                              GACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCT                 >  SRR4302540.6921654/1‑94 (MQ=60)
                                                                              GACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCT                 >  SRR4302540.1221123/1‑94 (MQ=60)
                                                                              GACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCT                 >  SRR4302540.4345906/1‑94 (MQ=60)
                                                                              GACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCT                 >  SRR4302540.1995625/1‑94 (MQ=60)
                                                                               ACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTC                >  SRR4302540.9092602/1‑94 (MQ=60)
                                                                               ACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTC                <  SRR4302540.6170860/94‑1 (MQ=60)
                                                                               ACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTC                >  SRR4302540.9958005/1‑94 (MQ=60)
                                                                                CGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCG               >  SRR4302540.9896019/1‑94 (MQ=60)
                                                                                CGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCG               <  SRR4302540.954902/94‑1 (MQ=60)
                                                                                CGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCG               <  SRR4302540.474609/94‑1 (MQ=60)
                                                                                 GCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGC              <  SRR4302540.7020298/94‑1 (MQ=60)
                                                                                 GCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGC              <  SRR4302540.9118273/94‑1 (MQ=60)
                                                                                 GCCAGTGGAAGCGGATGCCTTGCTTATTGCTGTGCCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGC              <  SRR4302540.7020299/94‑1 (MQ=60)
                                                                                  CCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCT             <  SRR4302540.1677587/94‑1 (MQ=60)
                                                                                  CCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCT             <  SRR4302540.9761153/94‑1 (MQ=60)
                                                                                  CCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCT             <  SRR4302540.855284/94‑1 (MQ=60)
                                                                                   CAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTC            <  SRR4302540.9827132/94‑1 (MQ=60)
                                                                                    AGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCC           >  SRR4302540.2308223/1‑94 (MQ=60)
                                                                                    AGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCC           >  SRR4302540.2308225/1‑94 (MQ=60)
                                                                                    AGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCC           >  SRR4302540.679940/1‑94 (MQ=60)
                                                                                    AGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCC           <  SRR4302540.7647968/94‑1 (MQ=60)
                                                                                     GTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCC          >  SRR4302540.9015001/1‑94 (MQ=60)
                                                                                     GTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCC          >  SRR4302540.2700758/1‑94 (MQ=60)
                                                                                     GTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCA          >  SRR4302540.4274409/1‑94 (MQ=60)
                                                                                      TTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCAT         <  SRR4302540.10031568/94‑1 (MQ=60)
                                                                                      TTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCAT         <  SRR4302540.4416584/94‑1 (MQ=60)
                                                                                       TGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATT        <  SRR4302540.8955508/94‑1 (MQ=60)
                                                                                       TGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATT        <  SRR4302540.7011037/94‑1 (MQ=60)
                                                                                       TGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATT        <  SRR4302540.278514/94‑1 (MQ=60)
                                                                                        GAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCTATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTG       >  SRR4302540.9152114/1‑94 (MQ=60)
                                                                                        GAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTG       <  SRR4302540.9715941/94‑1 (MQ=60)
                                                                                        GAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTG       <  SRR4302540.4186301/94‑1 (MQ=60)
                                                                                         AAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGC      <  SRR4302540.2744890/94‑1 (MQ=60)
                                                                                         AAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGC      <  SRR4302540.3146766/94‑1 (MQ=60)
                                                                                         AAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGC      <  SRR4302540.1379135/94‑1 (MQ=60)
                                                                                          AGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCG     <  SRR4302540.3985024/94‑1 (MQ=60)
                                                                                             GGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCA  <  SRR4302540.191551/94‑1 (MQ=60)
                                                                                             GGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCA  >  SRR4302540.1159309/1‑94 (MQ=60)
                                                                                             GGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCA  <  SRR4302540.1423960/94‑1 (MQ=60)
                                                                                                 |                                                                                         
ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGC‑‑‑‑ACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCA  >  NC_000913/3969010‑3969192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑