Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 245 579.6 28867798 97.7% 28203838 96.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,969,103 2 bp→GC coding (232‑233/1263 nt) wecC → UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,969,1030CG100.0% 1967.8 / NA 513R78A (CGC→GCC) wecCUDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (255/258);  total (255/258)
*NC_0009133,969,1040GC99.6% 1875.9 / NA 493R78A (CGC→GCC) wecCUDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (245/245);  minor base T (2/0);  total (247/246)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 530 total aligned reads displayed.

CATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCAG  >  NC_000913/3969013‑3969193
                                                                                          ||                                                                                         
 atatCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggc                                                                                      >  2:6837780/1‑96 (MQ=255)
  tatCGTCGAACCTGATTTGGCGAGTGTAGTAAAACCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggct                                                                                     <  1:1002209/96‑1 (MQ=255)
  tatCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggct                                                                                     >  1:8181963/1‑96 (MQ=255)
  tatCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggct                                                                                     <  2:8158214/96‑1 (MQ=255)
  tatCGTCGAACCTGATATGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggct                                                                                     <  1:10341980/96‑1 (MQ=255)
    tcgtcgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGa                                                                                   <  2:5161461/96‑1 (MQ=255)
     cgtcgACCCAGATTTGCCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAt                                                                                  <  1:7867574/96‑1 (MQ=255)
      gtcgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAtt                                                                                 >  2:8974609/1‑96 (MQ=255)
      gtcgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAtt                                                                                 <  2:8507990/96‑1 (MQ=255)
      gtcgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAtt                                                                                 >  2:2479983/1‑96 (MQ=255)
       tcgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTg                                                                                >  2:5517426/1‑96 (MQ=255)
       tcgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTg                                                                                >  1:1740494/1‑96 (MQ=255)
        cgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc                                                                               >  1:2864882/1‑96 (MQ=255)
        cgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc                                                                               >  1:6668602/1‑96 (MQ=255)
         gAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCt                                                                              >  1:5318018/1‑96 (MQ=255)
         gAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTAGCTGATTGCt                                                                              >  1:13943715/1‑96 (MQ=255)
          aaCCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTg                                                                             >  1:11958144/1‑96 (MQ=255)
            ccTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTa                                                                           <  1:566183/96‑1 (MQ=255)
              tGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGAGGCCTGGCTGATTGCTGTAcc                                                                         >  1:35607/1‑96 (MQ=255)
              tGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGGTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAcc                                                                         >  2:5635618/1‑96 (MQ=255)
               gATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAccc                                                                        <  1:2253814/96‑1 (MQ=255)
                aTTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCa                                                                       >  1:13539970/1‑96 (MQ=255)
                    ggCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGcc                                                                   >  2:10943182/1‑96 (MQ=255)
                    gacGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGGCGTACCCAAGcc                                                                   >  1:11103104/3‑96 (MQ=255)
                     gtgAGTATAGTAAAAACTACCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCg                                                                  <  1:6011081/94‑1 (MQ=255)
                     gCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCg                                                                  >  1:455832/1‑96 (MQ=255)
                      cGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGTGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGt                                                                 >  1:13019198/1‑96 (MQ=255)
                      cGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGt                                                                 >  1:129050/1‑96 (MQ=255)
                      cGAGGGTAGTAAAAACTGCCGGAGAAGGCGGTTTTTTACGAGCGATCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCGCGCCGt                                                                 >  1:5889890/1‑96 (MQ=255)
                       gAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAACCACGCCGtt                                                                >  2:9298994/1‑96 (MQ=255)
                        aGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCCCGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTTCCCACGCCGttt                                                               <  1:6488472/96‑1 (MQ=255)
                        aGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGttt                                                               >  1:4044776/1‑96 (MQ=255)
                        aGTGTAGTAAAAACAAACGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGGACCCACGCCGttt                                                               >  1:12378405/1‑96 (MQ=255)
                         gtgtAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAGTGCTCTGCCCACGCCGTTTa                                                              >  1:4670753/1‑96 (MQ=255)
                          tgtATTAAAAACTGCGGTAGAGGGCGTTTTTTTACGAGCGGGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTaa                                                             <  2:346911/96‑1 (MQ=255)
                           gtagtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAg                                                            >  1:6733561/1‑96 (MQ=255)
                            tagtaAAAACTGCCGTAGAAGGCGGTTTTTTAGGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAgg                                                           <  2:2894189/96‑1 (MQ=255)
                            tagaaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAgg                                                           <  2:4002635/96‑1 (MQ=255)
                              gtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGc                                                         <  1:5487715/96‑1 (MQ=255)
                              gaaaaacCTGCCGAAGAGGGCGTTTTTTAACAGGCAGGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGc                                                         <  2:2373864/94‑1 (MQ=255)
                                aaaaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGa                                                       >  1:1621444/1‑96 (MQ=255)
                                aaaaaCTGCCGTAAAAGGCGTTTTTTTACGTGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGa                                                       <  1:4987450/96‑1 (MQ=255)
                                 aaaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGAt                                                      >  1:8479288/1‑96 (MQ=255)
                                     cTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATg                                                  <  1:512143/96‑1 (MQ=255)
                                     cTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATg                                                  <  2:11921885/96‑1 (MQ=255)
                                        ccGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGc                                               <  2:7302965/96‑1 (MQ=255)
                                         cGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGcc                                              >  1:9528577/1‑96 (MQ=255)
                                           tAGAAGGCGGTTTTTTACGAGCGAGCCCGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTTTACCCACGCCGTTTAAGGGCGATCATGAGCCAg                                            <  2:6636668/96‑1 (MQ=255)
                                             gAAGGCGGTTTTTTACGAGCGAGCACGACGACAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGAGATCATGAGCCAGat                                          >  1:720378/1‑96 (MQ=255)
                                              aaGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata                                         <  1:14344063/96‑1 (MQ=255)
                                                   ggTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAcc                                    <  2:14241390/96‑1 (MQ=255)
                                                      tgtttACGAGCGACCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTCCCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAc                                 <  2:9614133/94‑1 (MQ=255)
                                                       ttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACg                                <  2:11051983/96‑1 (MQ=255)
                                                       ttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACg                                <  2:7736502/96‑1 (MQ=255)
                                                       ttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACg                                >  2:1370510/1‑96 (MQ=255)
                                                       ttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACg                                <  2:13961694/96‑1 (MQ=255)
                                                       tattACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACg                                >  1:10827802/3‑96 (MQ=255)
                                                        tatACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGt                               >  1:10689662/3‑96 (MQ=255)
                                                          tACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTg                             <  2:3186604/96‑1 (MQ=255)
                                                          tACGAGCGAGCACGAAGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTg                             <  1:4286705/96‑1 (MQ=255)
                                                            cgagcgagCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGTGCGATCATGAGCCAGATATGACCTACGTTGaa                           >  1:8802895/1‑96 (MQ=255)
                                                            cgagcgagCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGaa                           <  2:362398/96‑1 (MQ=255)
                                                            cgagcgagCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGaa                           <  2:6085922/96‑1 (MQ=255)
                                                            cgagcgagCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGaa                           >  2:14361503/1‑96 (MQ=255)
                                                             gagcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAt                          >  2:12619526/1‑96 (MQ=255)
                                                              agcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATc                         <  2:8276070/96‑1 (MQ=255)
                                                               gcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCg                        <  1:8674675/96‑1 (MQ=255)
                                                               gcatgcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCg                        <  1:975947/92‑1 (MQ=255)
                                                                cgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCgg                       >  2:1122880/1‑96 (MQ=255)
                                                                cgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCgg                       <  2:1740494/96‑1 (MQ=255)
                                                                 gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgc                      >  2:10490743/1‑96 (MQ=255)
                                                                 gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAAGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgc                      >  2:4173255/1‑96 (MQ=255)
                                                                  agcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgct                     >  1:6264954/1‑96 (MQ=255)
                                                                   gcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctg                    >  1:11254352/1‑96 (MQ=255)
                                                                   gcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctg                    >  2:12227714/1‑96 (MQ=255)
                                                                   gcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGATTAAGGGCGATCATGAGCCAGATATGACCTCCGTTGAATCGgctg                    <  2:13138967/96‑1 (MQ=255)
                                                                     acgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctgct                  <  1:6980543/96‑1 (MQ=255)
                                                                     acgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctgct                  >  1:13293408/1‑96 (MQ=255)
                                                                     acgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctgct                  >  1:3333457/1‑96 (MQ=255)
                                                                      cgcggCCAGTTGAAGCGGATGACTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctc                 <  2:461780/92‑1 (MQ=255)
                                                                      cgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctc                 <  1:11843980/96‑1 (MQ=255)
                                                                      cgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctc                 <  1:11438729/96‑1 (MQ=255)
                                                                      cgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctc                 >  2:1950778/1‑96 (MQ=255)
                                                                      cgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctc                 >  1:14055378/1‑96 (MQ=255)
                                                                       gacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCTCGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcg                <  2:5889890/96‑1 (MQ=255)
                                                                       gacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACACACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcg                >  2:10170619/1‑96 (MQ=255)
                                                                        acgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTGAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgcccgc               >  2:10304652/1‑96 (MQ=255)
                                                                         cgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcgct              <  2:2132005/96‑1 (MQ=255)
                                                                           ccAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTcc            >  2:7103278/1‑96 (MQ=255)
                                                                            cAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCa           >  2:2579081/1‑96 (MQ=255)
                                                                            cAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCa           >  1:9114346/1‑96 (MQ=255)
                                                                            cAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCACGCGCCa           >  1:3021311/1‑96 (MQ=255)
                                                                            cAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCGGCTCGCTCCa           >  2:5363480/1‑96 (MQ=255)
                                                                             aGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTccct          >  2:8653311/1‑94 (MQ=255)
                                                                              gTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCAtt         <  2:9122665/96‑1 (MQ=255)
                                                                              gTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCAtt         <  2:1621444/96‑1 (MQ=255)
                                                                              gTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCAtt         <  2:13410310/96‑1 (MQ=255)
                                                                               ttGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTg        >  1:255752/1‑96 (MQ=255)
                                                                                tGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTgc       <  2:11638962/96‑1 (MQ=255)
                                                                                 gAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTgcg      >  2:7938889/1‑96 (MQ=255)
                                                                                          ||                                                                                         
CATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCAG  >  NC_000913/3969013‑3969193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 294 total aligned reads displayed.
TGGCGAAATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCAGTGCTGA  >  NC_000913/3969003‑3969199
                                                                                                     |                                                                                                
TGGCGAAATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACAGCCGTAGAAGGCGGGTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAACGGATG                                                                                                  >  SRR4302518.7148853/1‑101 (MQ=60)
 GGCGAAATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCCCGACGCCAGTTGAAGCGGANNN                                                                                                 >  SRR4302518.14622149/1‑101 (MQ=60)
     AAATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGG                                                                                             <  SRR4302518.7029914/101‑1 (MQ=60)
     AAATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGG                                                                                             <  SRR4302518.4540147/101‑1 (MQ=60)
     AAATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGG                                                                                             >  SRR4302518.37877/1‑101 (MQ=60)
       NNNCATATCGTCGAACCTGATATGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCT                                                                                           <  SRR4302518.10828562/101‑1 (MQ=60)
       ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAACCTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCT                                                                                           <  SRR4302518.1041455/101‑1 (MQ=60)
          NNNATCGTCGAACCTGATTTGGCGAGTGTAGTAAAACCTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAT                                                                                        <  SRR4302518.13986948/101‑1 (MQ=60)
          CATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCCGTTGAAACGGAGGCCTGGCTGAT                                                                                        >  SRR4302518.4618189/1‑101 (MQ=60)
          CATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAT                                                                                        <  SRR4302518.5473492/101‑1 (MQ=60)
           ATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATT                                                                                       >  SRR4302518.2046895/1‑101 (MQ=60)
            TATCGTCGGACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGANNN                                                                                      >  SRR4302518.13585854/1‑101 (MQ=60)
            TATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGANNN                                                                                      >  SRR4302518.8586824/1‑101 (MQ=60)
             ATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATNNN                                                                                     >  SRR4302518.8591805/1‑101 (MQ=60)
              NNNTCGATCCTGATTTGGCGAGTGTAGTAATACCTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCTCGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCT                                                                                    <  SRR4302518.11724227/101‑1 (MQ=60)
               ccgcgatcctgtTTTGGCGAGTGTAGTAAAAACTGCCGAAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTG                                                                                   <  SRR4302518.515936/89‑1 (MQ=60)
                NNNGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGT                                                                                  <  SRR4302518.13669542/101‑1 (MQ=60)
                GTCGAACCTGATTTGGCGCGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCNNN                                                                                  >  SRR4302518.9016769/1‑101 (MQ=60)
                GTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTTGCTGATTGCTGT                                                                                  >  SRR4302518.5961539/1‑101 (MQ=60)
                GTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGT                                                                                  >  SRR4302518.5917310/1‑101 (MQ=60)
                GTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGT                                                                                  >  SRR4302518.6357920/1‑101 (MQ=60)
                GTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCNNN                                                                                  >  SRR4302518.12463445/1‑101 (MQ=60)
                 TCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTA                                                                                 <  SRR4302518.1005970/101‑1 (MQ=60)
                  ggacccggtttgGGCGAGTGTAGAAAAAACTGCCGTAGAAGGCGTTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAC                                                                                <  SRR4302518.2682896/89‑1 (MQ=60)
                  TGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGNNN                                                                                >  SRR4302518.14409446/1‑101 (MQ=60)
                  CGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAC                                                                                >  SRR4302518.2999930/1‑101 (MQ=60)
                  CGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTGGAAGCGGATGCCTGGCTGATTGCTGNNN                                                                                >  SRR4302518.13130724/1‑101 (MQ=60)
                   GAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACC                                                                               >  SRR4302518.6603908/1‑101 (MQ=60)
                   GAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACC                                                                               <  SRR4302518.5612562/101‑1 (MQ=60)
                   GAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACC                                                                               >  SRR4302518.5590782/1‑101 (MQ=60)
                   GAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACC                                                                               >  SRR4302518.5050965/1‑101 (MQ=60)
                    NNNCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCC                                                                              <  SRR4302518.13602974/101‑1 (MQ=60)
                    AACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTANNN                                                                              >  SRR4302518.12528401/1‑101 (MQ=60)
                    AACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCC                                                                              <  SRR4302518.2274492/101‑1 (MQ=60)
                    AACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCC                                                                              <  SRR4302518.819926/101‑1 (MQ=60)
                     ACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACNNN                                                                             >  SRR4302518.9189934/1‑101 (MQ=60)
                     ACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCA                                                                             <  SRR4302518.7343660/101‑1 (MQ=60)
                      NNNGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAT                                                                            <  SRR4302518.11832356/101‑1 (MQ=60)
                      CCTGATTTGGCGAGGGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCGGGCTGATTGCTGTA‑‑CACGC                                                                          >  SRR4302518.5803081/1‑101 (MQ=60)
                      CCGGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAC                                                                            <  SRR4302518.5705874/101‑1 (MQ=60)
                       NNNATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACG                                                                           <  SRR4302518.12207364/101‑1 (MQ=60)
                       NNNATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACG                                                                           <  SRR4302518.8907359/101‑1 (MQ=60)
                       CTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACG                                                                           <  SRR4302518.4355753/101‑1 (MQ=60)
                        nnngtatgtgcagtgtaaaaaatattgcctgtggcggcGTTTTTTACGGAGCGAGCCCGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTTCCCACG                                                                           <  SRR4302518.12589554/63‑1 (MQ=35)
                        TGATTTGGCGAGTGTAGTAAAACATGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGC                                                                          <  SRR4302518.6122110/101‑1 (MQ=60)
                        TGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGAGGCCTGGCTGATTGCTGTACCCCCGC                                                                          >  SRR4302518.37023/1‑101 (MQ=60)
                         tattgggcgggtgaAGTAAAAATTGCCGTAGAAGGCGGTTTTTTAG‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCC                                                                         <  SRR4302518.5464905/87‑1 (MQ=60)
                         NNNTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCC                                                                         <  SRR4302518.10936503/101‑1 (MQ=60)
                         GTTTTGGCGAGTGTAGTAAAAACTGCCGTAGAGGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCC                                                                         <  SRR4302518.5318807/101‑1 (MQ=60)
                         GATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACNNN                                                                         >  SRR4302518.14251550/1‑101 (MQ=60)
                          ATTTGGCGAGTGTGGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGNNN                                                                        >  SRR4302518.11489749/1‑101 (MQ=60)
                           TTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGT                                                                       >  SRR4302518.5774051/1‑101 (MQ=60)
                           TTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGT                                                                       >  SRR4302518.6152801/1‑101 (MQ=60)
                            nnnggGAGTGTAGTAAAACCTGCCGTAGAAGGCGGTTTTTTAC‑GAGGGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTT                                                                      <  SRR4302518.10413811/96‑1 (MQ=60)
                            TTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCNNN                                                                      >  SRR4302518.13920474/1‑101 (MQ=60)
                            TAGGCGAGTGTAGTAAAAACTGCCGGAGAAGGCGGTTTTTTAC‑GAGCGAGCACGGCGCCAGTTGAAGCAGATGCCTGGCTGATGCCTGTACCCACGCCNNN                                                                      >  SRR4302518.11239994/1‑101 (MQ=60)
                            NNNGCGAGTGTAGTAAAAACTGCCGTAGAGGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGCT                                                                      <  SRR4302518.11208677/101‑1 (MQ=60)
                             TGGCTAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCCCGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTTCCCACGCCGTTT                                                                     <  SRR4302518.6820678/101‑1 (MQ=60)
                              GTCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGGTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTTTACCCACTCCGTNNN                                                                    >  SRR4302518.11233125/1‑101 (MQ=60)
                              GGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTNNN                                                                    >  SRR4302518.10149956/1‑101 (MQ=60)
                              GACGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGGCGTACCCAAGCCGTNNN                                                                    >  SRR4302518.11622523/1‑101 (MQ=60)
                               GCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTgag                                                                   >  SRR4302518.473859/1‑98 (MQ=60)
                               GCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTNNN                                                                   >  SRR4302518.7426807/1‑101 (MQ=60)
                                nnngtgtagcaaaacctgcaccAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAG                                                                  <  SRR4302518.13387272/79‑1 (MQ=60)
                                NNNGTGTAGTAAAAACTGCCTTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAG                                                                  <  SRR4302518.10859786/101‑1 (MQ=60)
                                CGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGTGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTNNN                                                                  >  SRR4302518.13622145/1‑101 (MQ=60)
                                CGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACTCACGCCGTTTNNN                                                                  >  SRR4302518.12469711/1‑101 (MQ=60)
                                CGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAG                                                                  <  SRR4302518.7034141/101‑1 (MQ=60)
                                CGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAG                                                                  >  SRR4302518.735814/1‑101 (MQ=60)
                                CGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGAACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCGGTTTAGG                                                                  >  SRR4302518.7133442/1‑101 (MQ=60)
                                CGAGGGTAGTAAAAACTGCCGGAGAAGGCGGTTTTTTAC‑GAGCGATCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCGAGCCGTTTAAG                                                                  >  SRR4302518.6188376/1‑101 (MQ=60)
                                 GAGTGTATAAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGG                                                                 <  SRR4302518.3163039/101‑1 (MQ=60)
                                 GAGTGTAGTAAAACCTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGG                                                                 <  SRR4302518.1177508/101‑1 (MQ=60)
                                 GAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGG                                                                 >  SRR4302518.899157/1‑101 (MQ=60)
                                 GAGTGTAGTAAAAACTGCCGTAGAAGGCGGGTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGG                                                                 >  SRR4302518.5902890/1‑101 (MQ=60)
                                  NNNGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGG                                                                <  SRR4302518.13957721/101‑1 (MQ=60)
                                  CGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCGGTACCCACGCCtctcaannn                                                                >  SRR4302518.11936971/1‑92 (MQ=60)
                                  AGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAANNN                                                                >  SRR4302518.10036680/1‑101 (MQ=60)
                                  AGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAANNN                                                                >  SRR4302518.8684002/1‑101 (MQ=60)
                                  AGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAANNN                                                                >  SRR4302518.8710499/1‑101 (MQ=60)
                                  AGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGG                                                                >  SRR4302518.4239625/1‑101 (MQ=60)
                                  AGTGTAGTAAAAACAAACGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGGACCCACGCCGTTTAANNN                                                                >  SRR4302518.12962210/1‑101 (MQ=60)
                                   GTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTTCTGTACCCACGCCGTTTAAGTGC                                                               >  SRR4302518.7129769/1‑101 (MQ=60)
                                   GTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGNNN                                                               >  SRR4302518.13754099/1‑101 (MQ=60)
                                   GTGAAATAAAAACTGCCGTATAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGC                                                               <  SRR4302518.3137277/101‑1 (MQ=60)
                                     NNNGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGA                                                             <  SRR4302518.9146515/101‑1 (MQ=60)
                                     GGAGTAAAAACTGCCGTAAAAGGCGTTTTTTTAC‑GTGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGA                                                             <  SRR4302518.5246523/101‑1 (MQ=60)
                                      TAGTAAAACCTGCCGTAGAAGTCGGTTTTCTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGAT                                                            <  SRR4302518.5286991/101‑1 (MQ=60)
                                      TAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCNNN                                                            >  SRR4302518.12803366/1‑101 (MQ=60)
                                        GTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCA                                                          >  SRR4302518.4898421/1‑101 (MQ=60)
                                        GTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGANNN                                                          >  SRR4302518.11352975/1‑101 (MQ=60)
                                        GTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGANNN                                                          >  SRR4302518.8435032/1‑101 (MQ=60)
                                        GTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAGTGCTGTACCCACGCCGTTTAAGGGCGATCT                                                          >  SRR4302518.179315/1‑101 (MQ=60)
                                          AAAATCTGCCGTAGAAGGCGGCTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATG                                                        <  SRR4302518.6973319/101‑1 (MQ=60)
                                          AAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCNNN                                                        >  SRR4302518.14665030/1‑101 (MQ=60)
                                          AAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATG                                                        >  SRR4302518.1692438/1‑101 (MQ=60)
                                          AAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATG                                                        <  SRR4302518.532279/101‑1 (MQ=60)
                                          AAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTGAGGGGCGATCAGG                                                        >  SRR4302518.1240542/1‑101 (MQ=60)
                                           nnncctgcggtggaagcCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGA                                                       <  SRR4302518.8188859/84‑1 (MQ=60)
                                           NNNACTCCCGTAGAGGGCGTTTTTTTCC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGA                                                       <  SRR4302518.7527200/101‑1 (MQ=60)
                                           AAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCANNN                                                       >  SRR4302518.13349778/1‑101 (MQ=60)
                                           AAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCANNN                                                       >  SRR4302518.8893749/1‑101 (MQ=60)
                                            NNNCTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAC                                                      <  SRR4302518.11962563/101‑1 (MQ=60)
                                              ACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCC                                                    <  SRR4302518.6667739/101‑1 (MQ=60)
                                              ACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCC                                                    >  SRR4302518.6848711/1‑101 (MQ=60)
                                               NNNCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCA                                                   <  SRR4302518.9385800/101‑1 (MQ=60)
                                               CTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGNNN                                                   >  SRR4302518.9972519/1‑101 (MQ=60)
                                               CTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGNNN                                                   >  SRR4302518.8395214/1‑101 (MQ=60)
                                               CTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCA                                                   <  SRR4302518.1815139/101‑1 (MQ=60)
                                                TGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAG                                                  <  SRR4302518.4226058/101‑1 (MQ=60)
                                                NNNCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCTG                                                  <  SRR4302518.12362957/101‑1 (MQ=60)
                                                 GCCGTAGAAGGCGGTTTTTGAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGA                                                 <  SRR4302518.6007426/101‑1 (MQ=60)
                                                  CCGTAGAAGGCGTTTTTTAAG‑GAGCGACCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGAT                                                <  SRR4302518.2747176/101‑1 (MQ=60)
                                                  CCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCGCGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCANNN                                                >  SRR4302518.11756412/1‑101 (MQ=60)
                                                  CCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCCCGCCGTTTAAGGGCGATCATGAGCCAGAT                                                <  SRR4302518.6334799/101‑1 (MQ=60)
                                                   NNNAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATA                                               <  SRR4302518.15008076/101‑1 (MQ=60)
                                                   CGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATA                                               <  SRR4302518.162040/101‑1 (MQ=60)
                                                    nnnaAAGGCGGTTTTTTAA‑GAGCGATCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATAT                                              <  SRR4302518.12857742/97‑1 (MQ=60)
                                                    NNNGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATTT                                              <  SRR4302518.12391925/101‑1 (MQ=60)
                                                    GTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATAT                                              <  SRR4302518.5692935/101‑1 (MQ=60)
                                                    GTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGANNN                                              >  SRR4302518.7967534/1‑101 (MQ=60)
                                                     TAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATG                                             >  SRR4302518.6254641/1‑101 (MQ=60)
                                                     TAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATG                                             <  SRR4302518.3393004/101‑1 (MQ=60)
                                                      AGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGA                                            >  SRR4302518.7296898/1‑101 (MQ=60)
                                                      AGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGGTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGC                                            >  SRR4302518.4761389/1‑101 (MQ=60)
                                                       GAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAC                                           >  SRR4302518.6996154/1‑101 (MQ=60)
                                                        AAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGNNN                                          >  SRR4302518.14600677/1‑101 (MQ=60)
                                                        AAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACC                                          <  SRR4302518.3553440/101‑1 (MQ=60)
                                                        AAGGCGGATTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGgtcattagcaaggtataacc                                          >  SRR4302518.1525442/1‑81 (MQ=60)
                                                         AGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGANNN                                         >  SRR4302518.9522423/1‑101 (MQ=60)
                                                         AGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCT                                         <  SRR4302518.3697582/101‑1 (MQ=60)
                                                          GGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTA                                        >  SRR4302518.5360530/1‑101 (MQ=60)
                                                          GGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTA                                        >  SRR4302518.6195340/1‑101 (MQ=60)
                                                          GGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTA                                        <  SRR4302518.1755582/101‑1 (MQ=60)
                                                           GCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAC                                       <  SRR4302518.2365683/101‑1 (MQ=60)
                                                              GTTTTTTAG‑GGGCGGCCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTT                                    <  SRR4302518.2468226/101‑1 (MQ=60)
                                                                 TTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAA                                 <  SRR4302518.6725154/101‑1 (MQ=60)
                                                                 TATTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTNNN                                 >  SRR4302518.11336217/1‑101 (MQ=60)
                                                                  TTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGNNN                                >  SRR4302518.14690617/1‑101 (MQ=60)
                                                                  TTTAA‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAT                                <  SRR4302518.3114820/101‑1 (MQ=60)
                                                                  TATAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGNNN                                >  SRR4302518.11191801/1‑101 (MQ=60)
                                                                   TTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTCCCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATC                               <  SRR4302518.4632622/101‑1 (MQ=60)
                                                                    tggcgggcggGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATC                               <  SRR4302518.4970767/91‑1 (MQ=60)
                                                                    NNN‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATTG                              <  SRR4302518.14938086/101‑1 (MQ=60)
                                                                    NNN‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCG                              <  SRR4302518.9890878/101‑1 (MQ=60)
                                                                    NNN‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCG                              <  SRR4302518.9096631/101‑1 (MQ=60)
                                                                      C‑TAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCNNN                            >  SRR4302518.11570492/1‑101 (MQ=60)
                                                                      C‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGTGCGATCATGAGCCAGATATGACCTACGTTGAATCNNN                            >  SRR4302518.9230115/1‑101 (MQ=60)
                                                                      A‑GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGC                            <  SRR4302518.4009914/101‑1 (MQ=60)
                                                                        NNNCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGTT                           <  SRR4302518.7766744/101‑1 (MQ=60)
                                                                        GGGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATNNGNCNNNNATGACCTnnnnnnnnnnnnnn                           >  SRR4302518.10989730/1‑87 (MQ=60)
                                                                        GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGNNN                           >  SRR4302518.9567196/1‑101 (MQ=60)
                                                                        GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCT                           <  SRR4302518.2405878/101‑1 (MQ=60)
                                                                        GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCT                           <  SRR4302518.3585064/101‑1 (MQ=60)
                                                                         AGCGAGGACGACGCCAGTTGAAGCGGAGGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTG                          >  SRR4302518.3338894/1‑101 (MQ=60)
                                                                         AGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTG                          <  SRR4302518.7078310/101‑1 (MQ=60)
                                                                           NNNGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTCTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCT                        <  SRR4302518.10747114/101‑1 (MQ=60)
                                                                           CGAGCACGGCGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTNNN                        >  SRR4302518.13558180/1‑101 (MQ=60)
                                                                            NNNCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTC                       <  SRR4302518.9521365/101‑1 (MQ=60)
                                                                            GAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTC                       >  SRR4302518.4882844/1‑101 (MQ=60)
                                                                            GACCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTC                       <  SRR4302518.2582973/101‑1 (MQ=60)
                                                                             AGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCG                      >  SRR4302518.6584274/1‑101 (MQ=60)
                                                                             AGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCNNN                      >  SRR4302518.14274937/1‑101 (MQ=60)
                                                                             AGCACGACGCCAGTTGAAGCGGATGCCTGGATGAGTGCTGTACCAACGCCGTTTAAGGGCGATCGTGAGCCAGATATGACTTACGTGGAATCGGCTGgnnn                      >  SRR4302518.9379986/1‑97 (MQ=60)
                                                                              NNNCGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTGCCCACGCCGTTTAAGCGCGATCATGAGCAAGATATGACCTACGTTGAATCGGCTGCTCGC                     <  SRR4302518.10698684/101‑1 (MQ=60)
                                                                              GCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTNNN                     >  SRR4302518.11781087/1‑101 (MQ=60)
                                                                                ACGCCGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTC                   <  SRR4302518.4803541/101‑1 (MQ=60)
                                                                                ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGNNN                   >  SRR4302518.13905826/1‑101 (MQ=60)
                                                                                ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGNNN                   >  SRR4302518.11260617/1‑101 (MQ=60)
                                                                                ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTC                   >  SRR4302518.3488779/1‑101 (MQ=60)
                                                                                  GACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCA                 >  SRR4302518.1864408/1‑101 (MQ=60)
                                                                                   GCGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCAT                <  SRR4302518.2828462/101‑1 (MQ=60)
                                                                                   ACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCAT                >  SRR4302518.2898708/1‑101 (MQ=60)
                                                                                    nnnctGTTGAAGCGGATGCCTGGCTGATTTCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCACT               <  SRR4302518.10086561/96‑1 (MQ=60)
                                                                                    NNNCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATC               <  SRR4302518.14517677/101‑1 (MQ=60)
                                                                                    CGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATT               <  SRR4302518.6232709/101‑1 (MQ=60)
                                                                                     GCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCCCCANNN              >  SRR4302518.13501498/1‑101 (MQ=60)
                                                                                      NNNGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGC             <  SRR4302518.7576949/101‑1 (MQ=60)
                                                                                      CCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGGCCCATTGC             >  SRR4302518.3008722/1‑101 (MQ=60)
                                                                                      CCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATCGC             >  SRR4302518.5486504/1‑101 (MQ=60)
                                                                                       NNNTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCG            <  SRR4302518.14976648/101‑1 (MQ=60)
                                                                                       CGGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCCCGCTCCATTNNN            >  SRR4302518.12041368/1‑101 (MQ=60)
                                                                                       CAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCG            <  SRR4302518.2730422/101‑1 (MQ=60)
                                                                                       CAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCG            >  SRR4302518.4262146/1‑101 (MQ=60)
                                                                                        NNNTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGC           <  SRR4302518.9581845/101‑1 (MQ=60)
                                                                                        AGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTctgc           >  SRR4302518.5155488/1‑97 (MQ=60)
                                                                                         GTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCC          >  SRR4302518.2460981/1‑101 (MQ=60)
                                                                                         GTTGAAGCGGAGGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTGGCTCCATTGCGCC          >  SRR4302518.1593609/1‑101 (MQ=60)
                                                                                         GCTGAAGCGGATGCCTGGCTGAATGCTGTACACACGCCGAGTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGAC          <  SRR4302518.2287818/101‑1 (MQ=60)
                                                                                          TTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGGCA         >  SRR4302518.266073/1‑101 (MQ=60)
                                                                                          TTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCA         >  SRR4302518.1017150/1‑101 (MQ=60)
                                                                                           TGACGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCAG        <  SRR4302518.911023/101‑1 (MQ=60)
                                                                                            GAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCAGT       >  SRR4302518.4584177/1‑101 (MQ=60)
                                                                                            GAAGAGGATGCCTGGCTGTTTACTGTACCCACGCCGTTTCAGGGCGATCATGAGCCATATATGACCTCCGTTGAATCGGCTGCTCGCTCCATTGCGCCAGT       <  SRR4302518.5388147/101‑1 (MQ=60)
                                                                                             AAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCAGgc      >  SRR4302518.7067983/1‑99 (MQ=60)
                                                                                              AGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCAGTGC     <  SRR4302518.3699386/101‑1 (MQ=60)
                                                                                               GCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCAGTGCT    <  SRR4302518.4295219/101‑1 (MQ=60)
                                                                                                CGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCAGTGCTG   >  SRR4302518.639158/1‑101 (MQ=60)
                                                                                                CGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCGCGCTCCATGGCGCCAGTGNNN   >  SRR4302518.14855880/1‑101 (MQ=60)
                                                                                                 GGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCCCCATTGCGCCAGTGCTGA  >  SRR4302518.4439104/1‑101 (MQ=60)
                                                                                                     |                                                                                                
TGGCGAAATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTAC‑GAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCAGTGCTGA  >  NC_000913/3969003‑3969199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑