Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,969,429 C→T A186A (GCC→GCT wecC → UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,969,4290CT94.2% 1250.3 / 29.9 381A186A (GCC→GCTwecCUDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (13/9);  new base T (182/177);  total (195/186)
Fisher's exact test for biased strand distribution p-value = 5.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.69e-01

Only 100 of 409 total aligned reads displayed.

ACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCCCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGAAAA  >  NC_000913/3969338‑3969518
                                                                                           |                                                                                         
  tGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTcgc                                                                                        >  2:7106140/1‑93 (MQ=255)
    cccGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATTACGCCGGTTTGTTCGGACCgcgc                                                                                      >  2:6393883/1‑93 (MQ=255)
    cccGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCgcgc                                                                                      >  1:1688202/1‑93 (MQ=255)
     ccGGAACGCGTGTTAGCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCCCGCGcc                                                                                     <  1:7613466/93‑1 (MQ=255)
     ccGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGcc                                                                                     <  2:9437267/93‑1 (MQ=255)
      cGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCa                                                                                    >  1:9277412/1‑93 (MQ=255)
      cGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCCCGCGCCa                                                                                    >  2:4307784/1‑93 (MQ=255)
       ggAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGcccg                                                                                   >  2:4481756/1‑91 (MQ=255)
       ggAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAg                                                                                   <  2:4678884/93‑1 (MQ=255)
       ggAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAg                                                                                   >  1:9371450/1‑93 (MQ=255)
        gAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGGTCGCGCCCGc                                                                                  >  1:199795/1‑93 (MQ=255)
        gAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGc                                                                                  >  2:7890472/1‑93 (MQ=255)
        gAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGc                                                                                  >  2:9151659/1‑93 (MQ=255)
        gAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGc                                                                                  >  1:6542479/1‑93 (MQ=255)
         aaCGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCg                                                                                 >  1:7089169/1‑93 (MQ=255)
         aaCGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGAAGCCGGTTTGTTCGGCTCGCGCCAGCg                                                                                 >  2:3833938/1‑93 (MQ=255)
         aaCGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGGATGACGCCGGTTTGTTCGGCTCCCGCCACCg                                                                                 >  1:9451612/1‑93 (MQ=255)
          aCGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGa                                                                                <  1:7240825/93‑1 (MQ=255)
             cgTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACt                                                                             >  2:1057268/1‑93 (MQ=255)
             cgTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACt                                                                             <  1:4918739/93‑1 (MQ=255)
              gtgtTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTg                                                                            >  2:7723823/1‑93 (MQ=255)
               tgtTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGt                                                                           >  2:2516645/1‑93 (MQ=255)
               tgtTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGt                                                                           >  2:6899964/1‑93 (MQ=255)
                gtTACCAGGACAGGGAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGGACCGTa                                                                          >  2:4104578/1‑93 (MQ=255)
                 ttgccAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTAc                                                                         <  1:2882814/90‑1 (MQ=255)
                 ttACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTAc                                                                         >  2:8803240/1‑93 (MQ=255)
                 ttACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTAc                                                                         <  2:3120944/93‑1 (MQ=255)
                   aCCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACaa                                                                       <  1:803814/93‑1 (MQ=255)
                   aCCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACaa                                                                       <  2:819638/93‑1 (MQ=255)
                     cAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACaaaa                                                                     >  2:6155042/1‑93 (MQ=255)
                         aCAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCCCGCGCCAGCGAACTGTACAAAAtttt                                                                 >  2:7312012/1‑93 (MQ=255)
                          cAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTc                                                                >  1:7760794/1‑93 (MQ=255)
                           aGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTcc                                                               <  1:4645619/93‑1 (MQ=255)
                            ggTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCt                                                              <  1:5738604/93‑1 (MQ=255)
                             gTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGCACTGTACAAAATTTTACTc                                                             >  1:4138937/1‑93 (MQ=255)
                              tAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCg                                                            >  1:1549227/1‑93 (MQ=255)
                               aaTGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGa                                                           <  1:5021548/93‑1 (MQ=255)
                                aTGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGaa                                                          <  2:5397622/93‑1 (MQ=255)
                                aTGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGaa                                                          <  2:3588006/93‑1 (MQ=255)
                                  ggTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAACATTTTCCTCGAAgg                                                        >  2:9635555/1‑93 (MQ=255)
                                  ggTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAgg                                                        <  1:7034616/93‑1 (MQ=255)
                                   gTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGt                                                       <  2:548495/93‑1 (MQ=255)
                                    tCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTg                                                      <  1:7945614/93‑1 (MQ=255)
                                     cGAGCTGATTAAAATCGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGa                                                     <  1:232102/93‑1 (MQ=255)
                                     cGAGCTGATGAGAGGCGATCGCGTGATTGCTGGTACGACGCCGGTTTGTTCGCCCCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGa                                                     <  2:6285586/93‑1 (MQ=255)
                                       aGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAgt                                                   <  2:3286499/93‑1 (MQ=255)
                                       aGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAgt                                                   <  1:1463629/93‑1 (MQ=255)
                                       aGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACCCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAgt                                                   <  1:5444292/93‑1 (MQ=255)
                                        gCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAgtg                                                  >  2:1570994/1‑93 (MQ=255)
                                         cTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAgtgt                                                 >  1:9742381/1‑93 (MQ=255)
                                         cTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAgtgt                                                 >  2:6650980/1‑93 (MQ=255)
                                         cTGATTAAAAACGATCGCGGGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAgtgt                                                 <  1:7348219/93‑1 (MQ=255)
                                          tGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAgtgtg                                                >  1:2969085/1‑93 (MQ=255)
                                           gATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAgtgtgt                                               >  1:9163554/1‑93 (MQ=255)
                                              tAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTgtcgt                                            <  2:1427695/93‑1 (MQ=255)
                                                   aCGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTa                                       <  1:4475569/93‑1 (MQ=255)
                                                    cGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTaa                                      <  1:3611905/93‑1 (MQ=255)
                                                    cGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTaa                                      >  2:7056309/1‑93 (MQ=255)
                                                     gATCGGGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAAc                                     <  2:7959029/93‑1 (MQ=255)
                                                     gATCGCGTAATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAAc                                     <  2:8891143/93‑1 (MQ=255)
                                                      aTCGCGTGATTGGTTGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACt                                    <  2:2154741/93‑1 (MQ=255)
                                                      aTCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGGTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACt                                    >  1:696278/1‑93 (MQ=255)
                                                      aTCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGGGTCTCGGCACTAACt                                    >  1:3328088/1‑93 (MQ=255)
                                                        cgcgTGATTGGTGTTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGCACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACGAACTcg                                  <  1:5694741/93‑1 (MQ=255)
                                                            tGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGa                              >  1:2497367/1‑93 (MQ=255)
                                                            tGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCAAAGGTGAGTGTGTCGTCACTAACTCGCGGa                              <  1:5289010/93‑1 (MQ=255)
                                                              aTTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGAcg                            <  1:6856520/93‑1 (MQ=255)
                                                               ttGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGAcgg                           <  1:5679029/93‑1 (MQ=255)
                                                               ttGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGAcgg                           >  2:4789665/1‑93 (MQ=255)
                                                                tggtggTATGACGCGGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGAcggc                          <  2:453891/93‑1 (MQ=255)
                                                                 ggtggtATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGAcggcg                         <  1:5915626/93‑1 (MQ=255)
                                                                 ggtggtATGACGCCGGTTTGTTCGGCCCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGAcggcg                         >  1:1901424/1‑93 (MQ=255)
                                                                  gtggtATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGAcggcgg                        <  1:2803538/93‑1 (MQ=255)
                                                                   tggtATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCTTCACTAACTCGCGGACGGCGGa                       <  2:5264506/93‑1 (MQ=255)
                                                                    ggtATGACGCCGGTTTGTTCGGCTCGGGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGAGGGCGGaa                      >  1:3047303/1‑93 (MQ=255)
                                                                    ggtATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGaa                      <  1:10009354/93‑1 (MQ=255)
                                                                      tATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAAt                    >  1:1812318/1‑93 (MQ=255)
                                                                      tATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAAt                    >  2:3011424/1‑93 (MQ=255)
                                                                        tGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAAtgt                  <  1:4072054/93‑1 (MQ=255)
                                                                        tGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAAtgt                  >  2:1757157/1‑93 (MQ=255)
                                                                         gACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAAtgtg                 >  2:7630392/1‑93 (MQ=255)
                                                                         gACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAAtgtg                 <  2:1258082/93‑1 (MQ=255)
                                                                          aCGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATgtgt                >  2:5059063/1‑93 (MQ=255)
                                                                          aCGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATgtgt                <  1:882926/93‑1 (MQ=255)
                                                                          aCGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATgtgt                <  2:6932083/93‑1 (MQ=255)
                                                                           cGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTa               <  1:8463396/93‑1 (MQ=255)
                                                                           cGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTa               <  1:6305456/93‑1 (MQ=255)
                                                                           cGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTa               >  1:4195873/1‑93 (MQ=255)
                                                                           cGCCGGTTTGTTCGGCCCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTa               <  2:9837727/93‑1 (MQ=255)
                                                                            gCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGGCGGCGGGAATGTGTaa              >  1:6164439/1‑93 (MQ=255)
                                                                            gCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTaa              >  2:27238/1‑93 (MQ=255)
                                                                             ccGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAg             >  1:8638567/1‑93 (MQ=255)
                                                                              cGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGc            >  2:6350461/1‑93 (MQ=255)
                                                                                gTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTc          >  2:5889102/1‑93 (MQ=255)
                                                                                gTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTc          <  2:5750083/93‑1 (MQ=255)
                                                                                gTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTc          <  1:324062/93‑1 (MQ=255)
                                                                                gTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTc          >  1:1244915/1‑93 (MQ=255)
                                                                                    gTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCg      >  2:2381965/1‑93 (MQ=255)
                                                                                        ggCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCCCCGaaaa  <  2:8407328/93‑1 (MQ=255)
                                                                                        ggCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGaaaa  <  1:4728093/93‑1 (MQ=255)
                                                                                           |                                                                                         
ACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCCCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGAAAA  >  NC_000913/3969338‑3969518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 231 total aligned reads displayed.
TTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCCCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGAAAACAGC  >  NC_000913/3969336‑3969522
                                                                                             |                                                                                             
TTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCT                                                                                               <  SRR4302540.5999637/94‑1 (MQ=60)
TTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCT                                                                                               >  SRR4302540.4467988/1‑94 (MQ=60)
TTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCT                                                                                               <  SRR4302540.7610707/94‑1 (MQ=60)
TTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCT                                                                                               <  SRR4302540.1316480/94‑1 (MQ=60)
  ACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCG                                                                                             <  SRR4302540.6262075/94‑1 (MQ=60)
  ACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCG                                                                                             >  SRR4302540.8064835/1‑94 (MQ=60)
   CTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGTGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGC                                                                                            >  SRR4302540.937518/1‑94 (MQ=60)
     GCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGC                                                                                          >  SRR4302540.8078001/1‑94 (MQ=60)
     GCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGC                                                                                          >  SRR4302540.8521692/1‑94 (MQ=60)
     GCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGC                                                                                          >  SRR4302540.1706511/1‑94 (MQ=60)
      CCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCC                                                                                         >  SRR4302540.1034768/1‑94 (MQ=60)
       CCGGAACGCGTGTTAGCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCCCGCGCCA                                                                                        <  SRR4302540.7699051/94‑1 (MQ=60)
       CCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCC                                                                                        >  SRR4302540.8693617/1‑94 (MQ=60)
       CCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCA                                                                                        >  SRR4302540.9388319/1‑94 (MQ=60)
        CGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAG                                                                                       <  SRR4302540.9721330/94‑1 (MQ=60)
        CGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAG                                                                                       <  SRR4302540.4129767/94‑1 (MQ=60)
        CGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAG                                                                                       >  SRR4302540.9483025/1‑94 (MQ=60)
         ggagcggtTGTTACCAGTACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGC                                                                                      <  SRR4302540.4128221/86‑1 (MQ=60)
         GGAACGCTGGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGC                                                                                      <  SRR4302540.4215846/94‑1 (MQ=60)
         GGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCGGGTTTGTTCGGCTCGCGCCAGC                                                                                      <  SRR4302540.1838299/94‑1 (MQ=60)
         GGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGGTCGCGCCCGC                                                                                      >  SRR4302540.201767/1‑94 (MQ=60)
         GGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGC                                                                                      <  SRR4302540.4886899/94‑1 (MQ=60)
         GGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGC                                                                                      >  SRR4302540.6615079/1‑94 (MQ=60)
          GAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCG                                                                                     >  SRR4302540.7168056/1‑94 (MQ=60)
          GAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGGATGACGCCGGTTTGTTCGGCTCCCGCCACCG                                                                                     >  SRR4302540.9563799/1‑94 (MQ=60)
           AACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCCCGCGCCAGCGA                                                                                    >  SRR4302540.2758365/1‑94 (MQ=60)
            ACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGAGGCCGGTTTGTTCGGCTCGCGCCAGCGAT                                                                                   >  SRR4302540.9499913/1‑94 (MQ=60)
            ACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAA                                                                                   >  SRR4302540.9449167/1‑94 (MQ=60)
             CGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAAC                                                                                  <  SRR4302540.7807553/94‑1 (MQ=60)
              GCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACT                                                                                 >  SRR4302540.5068576/1‑94 (MQ=60)
               CGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTG                                                                                >  SRR4302540.8866332/1‑94 (MQ=60)
               CGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTG                                                                                <  SRR4302540.331983/94‑1 (MQ=60)
               CGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTG                                                                                <  SRR4302540.4971717/94‑1 (MQ=60)
               CGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTG                                                                                >  SRR4302540.6829690/1‑94 (MQ=60)
               CGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCCCGCGCCAGCGAACTG                                                                                <  SRR4302540.7048623/94‑1 (MQ=60)
                GTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAAatct                                                                               >  SRR4302540.1365302/1‑90 (MQ=60)
                GTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGT                                                                               <  SRR4302540.1847786/94‑1 (MQ=60)
                GTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGT                                                                               >  SRR4302540.6362065/1‑94 (MQ=60)
                GTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGT                                                                               >  SRR4302540.182700/1‑94 (MQ=60)
                GTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGT                                                                               >  SRR4302540.3956475/1‑94 (MQ=60)
                 TGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTA                                                                              >  SRR4302540.8461824/1‑94 (MQ=60)
                  GTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTAC                                                                             >  SRR4302540.6165736/1‑94 (MQ=60)
                  GTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTAC                                                                             <  SRR4302540.7129130/94‑1 (MQ=60)
                  GTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTAC                                                                             >  SRR4302540.7419805/1‑94 (MQ=60)
                   TTGCCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACA                                                                            <  SRR4302540.2914186/94‑1 (MQ=60)
                   TTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACA                                                                            <  SRR4302540.2914187/94‑1 (MQ=60)
                   TTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACA                                                                            <  SRR4302540.1638704/94‑1 (MQ=60)
                     ACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAA                                                                          >  SRR4302540.8713067/1‑94 (MQ=60)
                      CCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAA                                                                         <  SRR4302540.1018559/94‑1 (MQ=60)
                        AGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATT                                                                       >  SRR4302540.3021357/1‑94 (MQ=60)
                         GGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGCGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTTCAAAATTT                                                                      >  SRR4302540.5875029/1‑94 (MQ=60)
                           ACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTC                                                                    >  SRR4302540.7851484/1‑94 (MQ=60)
                            GAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCC                                                                   <  SRR4302540.7941574/94‑1 (MQ=60)
                             AGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCT                                                                  >  SRR4302540.775318/1‑94 (MQ=60)
                             AGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCT                                                                  <  SRR4302540.9529802/94‑1 (MQ=60)
                             AGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCT                                                                  <  SRR4302540.6988975/94‑1 (MQ=60)
                             AGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCT                                                                  <  SRR4302540.4695346/94‑1 (MQ=60)
                              GGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTC                                                                 <  SRR4302540.5801616/94‑1 (MQ=60)
                              GGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTC                                                                 >  SRR4302540.6503083/1‑94 (MQ=60)
                              GGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTC                                                                 <  SRR4302540.5801857/94‑1 (MQ=60)
                              GGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTC                                                                 >  SRR4302540.8039098/1‑94 (MQ=60)
                               GTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTTGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCCGCGAACTGTACACAATTTTCCTCG                                                                >  SRR4302540.7667100/1‑94 (MQ=60)
                               GTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCG                                                                >  SRR4302540.1566310/1‑94 (MQ=60)
                               GTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCG                                                                <  SRR4302540.4165583/94‑1 (MQ=60)
                               GTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCG                                                                >  SRR4302540.7667101/1‑94 (MQ=60)
                               GTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCG                                                                <  SRR4302540.4543828/94‑1 (MQ=60)
                                TAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGGCAGCGAACTGTACAAAATTTTCCTCGA                                                               >  SRR4302540.4012957/1‑94 (MQ=60)
                                TAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGA                                                               <  SRR4302540.889299/94‑1 (MQ=60)
                                TAATGGTCGAGCTGATTAAAAACGATCGCGCGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGA                                                               <  SRR4302540.5444082/94‑1 (MQ=60)
                                 AATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAA                                                              <  SRR4302540.3101739/94‑1 (MQ=60)
                                 AATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAA                                                              >  SRR4302540.5934947/1‑94 (MQ=60)
                                 AATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAA                                                              <  SRR4302540.4586886/94‑1 (MQ=60)
                                 AATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAA                                                              <  SRR4302540.5075905/94‑1 (MQ=60)
                                 AATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCCCGCGCCAGCGAACTGTACAAAATTTTCCTCGAG                                                              >  SRR4302540.2859177/1‑94 (MQ=60)
                                 AATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCCCGCGCCAGCGAACTGTACAAAATTTTCCTCGAA                                                              >  SRR4302540.2859178/1‑94 (MQ=60)
                                 AATGGCCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAA                                                              >  SRR4302540.7159728/1‑94 (MQ=60)
                                  ATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAG                                                             <  SRR4302540.4545467/94‑1 (MQ=60)
                                   TGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCCGCGAACTGTACAACATTCTCCTCGAAGG                                                            >  SRR4302540.3615985/1‑94 (MQ=60)
                                    GGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGCACTGTACAAAATTTTtctcgcaggg                                                           >  SRR4302540.7281412/1‑84 (MQ=60)
                                    GGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGT                                                           <  SRR4302540.6275811/94‑1 (MQ=60)
                                    GGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGT                                                           <  SRR4302540.7125326/94‑1 (MQ=60)
                                    GGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGT                                                           >  SRR4302540.5868296/1‑94 (MQ=60)
                                      TCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGA                                                         <  SRR4302540.8038228/94‑1 (MQ=60)
                                       TGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAG                                                        <  SRR4302540.6431981/94‑1 (MQ=60)
                                       CGAGCTGATTAAAATCGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAG                                                        <  SRR4302540.234344/94‑1 (MQ=60)
                                       CGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAG                                                        <  SRR4302540.9608687/94‑1 (MQ=60)
                                       CGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAG                                                        <  SRR4302540.6664243/94‑1 (MQ=60)
                                       CGAGCTGATTAAAAACGATCGCGTGATTGGTGGGATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTTCTCGAAGGTGAG                                                        >  SRR4302540.5371319/1‑94 (MQ=60)
                                        GAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGT                                                       <  SRR4302540.1957618/94‑1 (MQ=60)
                                         AGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTG                                                      >  SRR4302540.1625394/1‑94 (MQ=60)
                                         AGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTG                                                      <  SRR4302540.1479587/94‑1 (MQ=60)
                                         AGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTG                                                      <  SRR4302540.1479588/94‑1 (MQ=60)
                                         AGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTG                                                      <  SRR4302540.1479589/94‑1 (MQ=60)
                                         AGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCCCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTG                                                      >  SRR4302540.5742270/1‑94 (MQ=60)
                                         AGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACCCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTG                                                      <  SRR4302540.5504202/94‑1 (MQ=60)
                                          ggtgattaaacacggtcgcgtgatgtgtggtatgtgtcctGTTTGTGCGGCTCGCGCCAGCGAACGGTACAAAATTTTCCTCGAAGGTGAGTGT                                                     <  SRR4302540.2152253/54‑1 (MQ=54)
                                          GCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGT                                                     >  SRR4302540.9856728/1‑94 (MQ=60)
                                          GCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGT                                                     <  SRR4302540.9249828/94‑1 (MQ=60)
                                          GCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGT                                                     <  SRR4302540.1009152/94‑1 (MQ=60)
                                           CTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTG                                                    >  SRR4302540.3001878/1‑94 (MQ=60)
                                           CTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTG                                                    >  SRR4302540.5750396/1‑94 (MQ=60)
                                           CTGATTAAAAACGATCGCGTGATAGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTG                                                    >  SRR4302540.769712/1‑94 (MQ=60)
                                           CTGATTAAAAACGATCGCGGGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTG                                                    <  SRR4302540.7430199/94‑1 (MQ=60)
                                            TGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGT                                                   >  SRR4302540.9273517/1‑94 (MQ=60)
                                            CGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGT                                                   <  SRR4302540.3287387/94‑1 (MQ=60)
                                             GATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTC                                                  >  SRR4302540.1634147/1‑94 (MQ=60)
                                             GATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTC                                                  <  SRR4302540.1134332/94‑1 (MQ=60)
                                              ATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCG                                                 >  SRR4302540.5663144/1‑94 (MQ=60)
                                              ATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCG                                                 >  SRR4302540.1968763/1‑94 (MQ=60)
                                                TAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTC                                               >  SRR4302540.860512/1‑94 (MQ=60)
                                                 AAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCA                                              <  SRR4302540.4821301/94‑1 (MQ=60)
                                                  AAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCAC                                             >  SRR4302540.7598544/1‑94 (MQ=60)
                                                   AAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACT                                            >  SRR4302540.7223699/1‑94 (MQ=60)
                                                     ACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAA                                          <  SRR4302540.4524120/94‑1 (MQ=60)
                                                      CGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAAC                                         >  SRR4302540.5615020/1‑94 (MQ=60)
                                                      CGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAAC                                         <  SRR4302540.3651391/94‑1 (MQ=60)
                                                       GATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGGTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACT                                        >  SRR4302540.703751/1‑94 (MQ=60)
                                                       GATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACT                                        >  SRR4302540.6872008/1‑94 (MQ=60)
                                                       GATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACT                                        >  SRR4302540.2812771/1‑94 (MQ=60)
                                                       GATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACT                                        <  SRR4302540.9307376/94‑1 (MQ=60)
                                                       GATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGGGTCTCGGCACTAACT                                        >  SRR4302540.3364618/1‑94 (MQ=60)
                                                       GATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCCCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACT                                        >  SRR4302540.2385158/1‑94 (MQ=60)
                                                        ATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTC                                       <  SRR4302540.9286223/94‑1 (MQ=60)
                                                        ATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTC                                       <  SRR4302540.3225555/94‑1 (MQ=60)
                                                        ATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTC                                       >  SRR4302540.5003056/1‑94 (MQ=60)
                                                        ATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTC                                       <  SRR4302540.4225496/94‑1 (MQ=60)
                                                        ATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTC                                       <  SRR4302540.4225495/94‑1 (MQ=60)
                                                         TCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCG                                      <  SRR4302540.7084241/94‑1 (MQ=60)
                                                          CGCGTGATTGGTGTTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGCACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACGAACTCGC                                     <  SRR4302540.5757444/94‑1 (MQ=60)
                                                          CGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGC                                     >  SRR4302540.6044499/1‑94 (MQ=60)
                                                           GCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCG                                    <  SRR4302540.10053189/94‑1 (MQ=60)
                                                           GCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCG                                    <  SRR4302540.5777813/94‑1 (MQ=60)
                                                             GTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGA                                  >  SRR4302540.4338454/1‑94 (MQ=60)
                                                             GTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGA                                  >  SRR4302540.2524293/1‑94 (MQ=60)
                                                             GTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGA                                  >  SRR4302540.2524292/1‑94 (MQ=60)
                                                             GTGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGA                                  <  SRR4302540.2026113/94‑1 (MQ=60)
                                                              TGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGAC                                 <  SRR4302540.8649297/94‑1 (MQ=60)
                                                              TGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGAC                                 <  SRR4302540.5154850/94‑1 (MQ=60)
                                                              TGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGAC                                 >  SRR4302540.6778695/1‑94 (MQ=60)
                                                              TGATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCAAAGGTGAGTGTGTCGTCACTAACTCGCGGAC                                 <  SRR4302540.5346816/94‑1 (MQ=60)
                                                                ATTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGG                               <  SRR4302540.6932490/94‑1 (MQ=60)
                                                                 TTGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGC                              <  SRR4302540.5741556/94‑1 (MQ=60)
                                                                  TGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCCCTAACTCGCGGACGGCG                             >  SRR4302540.1372940/1‑94 (MQ=60)
                                                                  TGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCG                             <  SRR4302540.4695899/94‑1 (MQ=60)
                                                                  TGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCG                             <  SRR4302540.2942783/94‑1 (MQ=60)
                                                                  TGGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCG                             <  SRR4302540.3625254/94‑1 (MQ=60)
                                                                  TGGTGGTATGACGCCGGTTTGTTCGGCCCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCG                             >  SRR4302540.1922215/1‑94 (MQ=60)
                                                                   GGTGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGG                            <  SRR4302540.5980977/94‑1 (MQ=60)
                                                                     TGGTATGACGCCGGTTTGTTCGGCTCGGGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGAGGGCGGAA                          >  SRR4302540.3080741/1‑94 (MQ=60)
                                                                     TGGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAA                          >  SRR4302540.6230662/1‑94 (MQ=60)
                                                                      GGTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAA                         <  SRR4302540.10125908/94‑1 (MQ=60)
                                                                       GTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAAT                        <  SRR4302540.2973312/94‑1 (MQ=60)
                                                                       GTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAAT                        >  SRR4302540.1832096/1‑94 (MQ=60)
                                                                       GTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAAT                        >  SRR4302540.2912081/1‑94 (MQ=60)
                                                                       GTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAAT                        <  SRR4302540.306083/94‑1 (MQ=60)
                                                                       GTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAAT                        >  SRR4302540.3468540/1‑94 (MQ=60)
                                                                       GTATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAAT                        <  SRR4302540.7185143/94‑1 (MQ=60)
                                                                        TATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATG                       <  SRR4302540.6339347/94‑1 (MQ=60)
                                                                         ATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGT                      >  SRR4302540.9156939/1‑94 (MQ=60)
                                                                         ATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGT                      >  SRR4302540.4788121/1‑94 (MQ=60)
                                                                         ATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGT                      <  SRR4302540.9354870/94‑1 (MQ=60)
                                                                         ATGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGG                      >  SRR4302540.7357208/1‑94 (MQ=60)
                                                                          TGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTG                     <  SRR4302540.497646/94‑1 (MQ=60)
                                                                          TGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTG                     <  SRR4302540.2065487/94‑1 (MQ=60)
                                                                          TGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTG                     <  SRR4302540.4115949/94‑1 (MQ=60)
                                                                          TGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTG                     >  SRR4302540.5446786/1‑94 (MQ=60)
                                                                          TGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTG                     <  SRR4302540.4115950/94‑1 (MQ=60)
                                                                          TGACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTG                     <  SRR4302540.4394546/94‑1 (MQ=60)
                                                                          TGACGCCGGTTTGTTCGGCCCGCGCCAGCGGACTGTACAAAATTTTCCTCGAAGGCGAGGGGGTCGTCACTAACTCGCGGACGGCGGACATGTG                     >  SRR4302540.9309019/1‑94 (MQ=60)
                                                                           GACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGT                    >  SRR4302540.4844839/1‑94 (MQ=60)
                                                                            ACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTA                   >  SRR4302540.4241514/1‑94 (MQ=60)
                                                                            ACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTA                   <  SRR4302540.892291/94‑1 (MQ=60)
                                                                            ACGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTA                   <  SRR4302540.9474468/94‑1 (MQ=60)
                                                                             CGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAA                  <  SRR4302540.8561668/94‑1 (MQ=60)
                                                                             CGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAA                  <  SRR4302540.6375673/94‑1 (MQ=60)
                                                                             CGCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAA                  <  SRR4302540.5788414/94‑1 (MQ=60)
                                                                              GCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAG                 <  SRR4302540.4734105/94‑1 (MQ=60)
                                                                              GCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAG                 >  SRR4302540.3341349/1‑94 (MQ=60)
                                                                              GCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAG                 >  SRR4302540.8738912/1‑94 (MQ=60)
                                                                              GCCGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAG                 <  SRR4302540.5088646/94‑1 (MQ=60)
                                                                                CGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCT               <  SRR4302540.9767724/94‑1 (MQ=60)
                                                                                CGGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAAATCGCGGACGGCGGAAATGTGTgagat               >  SRR4302540.4729704/1‑89 (MQ=60)
                                                                                 GGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTC              >  SRR4302540.6959547/1‑94 (MQ=60)
                                                                                 GGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTC              >  SRR4302540.1257616/1‑94 (MQ=60)
                                                                                 GGTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTC              >  SRR4302540.8369893/1‑94 (MQ=60)
                                                                                  GTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCA             >  SRR4302540.1838261/1‑94 (MQ=60)
                                                                                  GTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCA             >  SRR4302540.8000399/1‑94 (MQ=60)
                                                                                  GTTTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCA             <  SRR4302540.327310/94‑1 (MQ=60)
                                                                                    TTGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACC           <  SRR4302540.2282548/94‑1 (MQ=60)
                                                                                     TGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCG          >  SRR4302540.8782094/1‑94 (MQ=60)
                                                                                     TGTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCG          >  SRR4302540.2399922/1‑94 (MQ=60)
                                                                                      GTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGA         >  SRR4302540.7098993/1‑94 (MQ=60)
                                                                                      GTTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGA         >  SRR4302540.3837171/1‑94 (MQ=60)
                                                                                       TTCGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGAA        >  SRR4302540.1825181/1‑94 (MQ=60)
                                                                                         CGGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGCGTGTGTCGTCACTAACTCGCCGACGGCGGAAATGTGTAAGCTCCCCGCAAA      >  SRR4302540.360682/1‑94 (MQ=60)
                                                                                          GGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGAAAAC     <  SRR4302540.4778588/94‑1 (MQ=60)
                                                                                          GGCTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGAAAAC     <  SRR4302540.301236/94‑1 (MQ=60)
                                                                                          GGCCCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGAAAAC     <  SRR4302540.7393657/94‑1 (MQ=60)
                                                                                            CTCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGAAAACAG   >  SRR4302540.5723964/1‑94 (MQ=60)
                                                                                             TCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGAAAACAGC  >  SRR4302540.5453248/1‑94 (MQ=60)
                                                                                             |                                                                                             
TTACTGCCCGGAACGCGTGTTACCAGGACAGGTAATGGTCGAGCTGATTAAAAACGATCGCGTGATTGGTGGTATGACGCCGGTTTGTTCGGCCCGCGCCAGCGAACTGTACAAAATTTTCCTCGAAGGTGAGTGTGTCGTCACTAACTCGCGGACGGCGGAAATGTGTAAGCTCACCGAAAACAGC  >  NC_000913/3969336‑3969522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: