Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 1548 369.5 20095359 96.9% 19472402 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,978,702 C→T S68F (TCC→TTC)  yifK → putative amino acid/amine transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,978,7020CT99.3% 1588.3 / NA 436S68F (TCC→TTC) yifKputative amino acid/amine transport protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (219/214);  minor base G (0/3);  total (219/217)
Fisher's exact test for biased strand distribution p-value = 1.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 465 total aligned reads displayed.

TGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTCCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGT  >  NC_000913/3978612‑3978791
                                                                                          |                                                                                         
tGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTtcc                                                                                         >  1:5341862/1‑93 (MQ=255)
tGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTtcc                                                                                         >  1:9569426/1‑93 (MQ=255)
tGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTtcc                                                                                         <  2:3551575/93‑1 (MQ=255)
 gAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCt                                                                                        <  1:5571128/93‑1 (MQ=255)
 gAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCt                                                                                        >  1:8436739/1‑93 (MQ=255)
 gAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCt                                                                                        <  1:8259276/93‑1 (MQ=255)
    aTGGGCCGGCCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGa                                                                                     <  1:2447372/93‑1 (MQ=255)
     tGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGaa                                                                                    <  2:3298062/93‑1 (MQ=255)
     tGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGaa                                                                                    <  2:4055489/93‑1 (MQ=255)
      gggccgggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAac                                                                                   <  2:1013663/93‑1 (MQ=255)
      gggccgggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAac                                                                                   <  2:2734448/93‑1 (MQ=255)
        gccgggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccg                                                                                 <  1:8590574/93‑1 (MQ=255)
        gccgggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccg                                                                                 <  2:6123709/93‑1 (MQ=255)
          cgggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggt                                                                               <  1:8125006/93‑1 (MQ=255)
            ggccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggtta                                                                             <  2:4904283/93‑1 (MQ=255)
             gccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttac                                                                            <  1:8563942/93‑1 (MQ=255)
             gccATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttac                                                                            >  1:6281212/1‑93 (MQ=255)
               cATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccg                                                                          >  2:5194010/1‑93 (MQ=255)
                aTCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGCAATGTTGTTTCTCGCaccggttaccgg                                                                         >  2:53095/1‑93 (MQ=255)
                  ccGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAaccggttaccggtt                                                                       <  1:10004693/93‑1 (MQ=255)
                   cGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttc                                                                      <  1:4939529/93‑1 (MQ=255)
                   cGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttc                                                                      <  1:591144/93‑1 (MQ=255)
                    gTATTGTTGGCCTATATAATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcg                                                                     <  1:7497828/93‑1 (MQ=255)
                     tATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgt                                                                    <  1:9097833/93‑1 (MQ=255)
                      aTTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgtt                                                                   >  1:7150618/1‑93 (MQ=255)
                       ttgttgGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgttc                                                                  >  2:2996225/1‑93 (MQ=255)
                       ttgttgGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgttc                                                                  >  1:4875604/1‑93 (MQ=255)
                       ttgttgGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCACCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGgttcgttc                                                                  <  2:382972/93‑1 (MQ=255)
                         gttgGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGc                                                                >  2:7247470/1‑93 (MQ=255)
                             gCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGtt                                                            <  2:117071/93‑1 (MQ=255)
                             gCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGtt                                                            <  2:235823/93‑1 (MQ=255)
                              ccTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGttt                                                           >  1:2248012/1‑93 (MQ=255)
                              ccTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGttt                                                           <  2:3090256/93‑1 (MQ=255)
                               cTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTa                                                          <  1:2897747/93‑1 (MQ=255)
                               cTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTa                                                          >  2:9217614/1‑93 (MQ=255)
                                tataTCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTAt                                                         >  2:2632991/1‑93 (MQ=255)
                                tataTCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTAt                                                         >  1:7909753/1‑93 (MQ=255)
                                tataTCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTAt                                                         <  1:9926239/93‑1 (MQ=255)
                                tataTCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTAt                                                         >  1:2699819/1‑93 (MQ=255)
                                  tatCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATgc                                                       <  1:9419928/93‑1 (MQ=255)
                                   atcatcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATgcg                                                      >  2:7302229/1‑93 (MQ=255)
                                    tcatcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATgcgc                                                     >  2:8402211/1‑93 (MQ=255)
                                     catcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCa                                                    >  2:8119469/1‑93 (MQ=255)
                                     catcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCa                                                    <  2:949975/93‑1 (MQ=255)
                                       tcGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATc                                                  >  1:4185774/1‑93 (MQ=255)
                                         gCCTGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGt                                                <  2:4294691/93‑1 (MQ=255)
                                          ccGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGtt                                               <  1:2780142/93‑1 (MQ=255)
                                           cGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTCCCGGTTCGTTCGCCGTTTATGCGCATCGTta                                              <  2:8591105/93‑1 (MQ=255)
                                            gggCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtat                                             <  2:9683787/93‑1 (MQ=255)
                                            gggCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtat                                             >  1:7757015/1‑93 (MQ=255)
                                            gggCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtat                                             >  2:2164171/1‑93 (MQ=255)
                                             ggCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGATATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtata                                            >  1:5397680/1‑93 (MQ=255)
                                             ggCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtata                                            >  1:5176871/1‑93 (MQ=255)
                                             ggCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTtata                                            <  1:3985885/93‑1 (MQ=255)
                                              gCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTatat                                           <  2:9851728/93‑1 (MQ=255)
                                                tGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGa                                         >  2:2543962/1‑93 (MQ=255)
                                                 gTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAg                                        <  2:1611194/93‑1 (MQ=255)
                                                 gTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAg                                        >  2:5959627/1‑93 (MQ=255)
                                                 gTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAg                                        >  1:4294410/1‑93 (MQ=255)
                                                 gTTCGCCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGCTCGTTCGCCGTTTTTGCGCCTCGTTATATTAg                                        >  2:7592375/1‑93 (MQ=255)
                                                  ttCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTCTATGAGc                                       >  2:7865331/1‑93 (MQ=255)
                                                  ttCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGc                                       <  2:6557467/93‑1 (MQ=255)
                                                  ttCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGc                                       >  1:4477857/1‑93 (MQ=255)
                                                  ttCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGc                                       <  1:3688536/93‑1 (MQ=255)
                                                   tCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGcc                                      >  2:3674784/1‑93 (MQ=255)
                                                   tCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGcc                                      <  1:661839/93‑1 (MQ=255)
                                                    tgTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGccc                                     <  2:1997361/92‑1 (MQ=255)
                                                    cGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGccc                                     >  2:4468561/1‑93 (MQ=255)
                                                     gTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCg                                    <  2:3353208/93‑1 (MQ=255)
                                                      tCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGt                                   >  1:703246/1‑93 (MQ=255)
                                                       cTTTTTCATCATGAGTTCATTGGACGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCgaa                                  <  1:8934135/93‑3 (MQ=255)
                                                        tttttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTc                                 <  1:6196268/93‑1 (MQ=255)
                                                         ttttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCt                                >  1:6423050/1‑93 (MQ=255)
                                                          tttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCtt                               >  2:7625203/1‑93 (MQ=255)
                                                           ttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCttt                              >  2:6979649/1‑93 (MQ=255)
                                                           ttCATCATGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCttt                              <  2:6781090/93‑1 (MQ=255)
                                                            tcatcaTGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTg                             <  1:1100641/93‑1 (MQ=255)
                                                              atcatGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGCTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGc                           >  1:1355024/1‑93 (MQ=255)
                                                                  tGCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAtc                       >  1:9824064/1‑93 (MQ=255)
                                                                  tGCGTTCAATGGGCGAAATGTTGGTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAtc                       <  2:5855347/93‑1 (MQ=255)
                                                                   gctgtCAATGTGCGATATGTTGTGCTTCGAACCTGTTCCCGGTTCGTTCGCCGTTTATGCGCAGCGTTATATGAGCCCGTTCTTTGGCTAtct                      <  1:3590564/89‑1 (MQ=255)
                                                                   gCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAtct                      <  2:9012423/93‑1 (MQ=255)
                                                                   gCGTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAtct                      <  2:7444116/93‑1 (MQ=255)
                                                                     gTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCa                    <  1:4643552/93‑1 (MQ=255)
                                                                     gTTCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCa                    <  1:7435067/93‑1 (MQ=255)
                                                                      ttCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCAc                   >  1:7684569/1‑93 (MQ=255)
                                                                      ttCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCAc                   >  2:5440188/1‑93 (MQ=255)
                                                                      ttCAAGGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCAc                   <  1:1877300/93‑1 (MQ=255)
                                                                       tCAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCAcc                  >  2:359411/1‑93 (MQ=255)
                                                                        cAATGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCg                 >  2:6270745/1‑93 (MQ=255)
                                                                          aTGGGCGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGcc               >  2:4688266/1‑93 (MQ=255)
                                                                               cGAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTc          <  2:565007/93‑1 (MQ=255)
                                                                                gAAATGTTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCt         >  2:4193775/1‑93 (MQ=255)
                                                                                    tgttgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACt     <  1:8747611/93‑1 (MQ=255)
                                                                                    tgttgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACt     <  1:3258359/93‑1 (MQ=255)
                                                                                    tgttgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACt     >  2:1414330/1‑93 (MQ=255)
                                                                                     gttgttCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTg    <  1:6521005/93‑1 (MQ=255)
                                                                                      ttgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTgg   <  1:9160671/93‑1 (MQ=255)
                                                                                      ttgtTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTgg   >  2:8791977/1‑93 (MQ=255)
                                                                                      ttgtGCCTCGAACCGGTTACCGGTGCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTgg   <  1:1662807/93‑1 (MQ=255)
                                                                                          |                                                                                         
TGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATGTTGTCCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGT  >  NC_000913/3978612‑3978791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 252 total aligned reads displayed.
CCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTCCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTAT  >  NC_000913/3978609‑3978795
                                                                                              |                                                                                             
 CCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGAGAAATG‑TTGTTC                                                                                              >  SRR4302540.5815291/1‑94 (MQ=60)
  CTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCC                                                                                             <  SRR4302540.4103403/94‑1 (MQ=60)
  CTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCC                                                                                             >  SRR4302540.5400449/1‑94 (MQ=60)
  CTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCC                                                                                             >  SRR4302540.9682493/1‑94 (MQ=60)
   TGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCT                                                                                            >  SRR4302540.2037140/1‑94 (MQ=60)
   TGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCT                                                                                            >  SRR4302540.8534685/1‑94 (MQ=60)
   CGAAAGGGGCCGGGCCATCCGTATTGTTGTCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCT                                                                                            <  SRR4302540.2900539/94‑1 (MQ=60)
    GAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTC                                                                                           <  SRR4302540.8355256/94‑1 (MQ=60)
    GAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTC                                                                                           <  SRR4302540.8247629/94‑1 (MQ=60)
    GAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTC                                                                                           <  SRR4302540.8355255/94‑1 (MQ=60)
     AAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCG                                                                                          >  SRR4302540.89333/1‑94 (MQ=60)
     AAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCG                                                                                          >  SRR4302540.2284336/1‑94 (MQ=60)
     AAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCG                                                                                          >  SRR4302540.1356258/1‑94 (MQ=60)
     AAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCG                                                                                          >  SRR4302540.1055943/1‑94 (MQ=60)
      AATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGA                                                                                         >  SRR4302540.8539213/1‑94 (MQ=60)
       ATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAA                                                                                        >  SRR4302540.760459/1‑94 (MQ=60)
       ATGGGCCGGCCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAA                                                                                        <  SRR4302540.2473725/94‑1 (MQ=60)
         GGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACC                                                                                      >  SRR4302540.6303420/1‑94 (MQ=60)
           GCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGG                                                                                    <  SRR4302540.8690442/94‑1 (MQ=60)
            CCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGT                                                                                   <  SRR4302540.7569370/94‑1 (MQ=60)
            CCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGT                                                                                   <  SRR4302540.8357808/94‑1 (MQ=60)
             CGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTT                                                                                  <  SRR4302540.8219306/94‑1 (MQ=60)
              GGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTA                                                                                 <  SRR4302540.868800/94‑1 (MQ=60)
               GGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGCACCGGTTAC                                                                                >  SRR4302540.6466243/1‑94 (MQ=60)
               GGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTAC                                                                                <  SRR4302540.2840717/94‑1 (MQ=60)
               GGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTAC                                                                                >  SRR4302540.6466244/1‑94 (MQ=60)
               GGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTAC                                                                                >  SRR4302540.6351111/1‑94 (MQ=60)
               GGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAACG‑TTGTTCCTCGAACCGGTTAC                                                                                >  SRR4302540.2533662/1‑94 (MQ=60)
               GGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGGCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTAC                                                                                <  SRR4302540.6549351/94‑1 (MQ=60)
                GCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACC                                                                               <  SRR4302540.8663396/94‑1 (MQ=60)
                 CCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACaggtacccg                                                                              >  SRR4302540.3484122/1‑85 (MQ=60)
                 CCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCG                                                                              >  SRR4302540.3484123/1‑94 (MQ=60)
                  CATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGCTTACCGG                                                                             >  SRR4302540.8556591/1‑94 (MQ=60)
                   ATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGT                                                                            >  SRR4302540.5081438/1‑94 (MQ=60)
                   ATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGT                                                                            >  SRR4302540.5564245/1‑94 (MQ=60)
                   ATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGT                                                                            >  SRR4302540.5852792/1‑94 (MQ=60)
                    TCCGTATTGTTTGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTT                                                                           <  SRR4302540.2713015/94‑1 (MQ=60)
                    TCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTT                                                                           <  SRR4302540.9169950/94‑1 (MQ=60)
                    TCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTT                                                                           >  SRR4302540.4844572/1‑94 (MQ=60)
                     CCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGTGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTC                                                                          >  SRR4302540.7623610/1‑94 (MQ=60)
                     CCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTC                                                                          <  SRR4302540.10121223/94‑1 (MQ=60)
                     CCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTC                                                                          <  SRR4302540.9181569/94‑1 (MQ=60)
                      CGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCG                                                                         <  SRR4302540.597513/94‑1 (MQ=60)
                      CGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCG                                                                         <  SRR4302540.1332165/94‑1 (MQ=60)
                       GTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGACCCGGTTACCGGTTCGT                                                                        >  SRR4302540.1462174/1‑94 (MQ=60)
                       GTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGT                                                                        >  SRR4302540.2857099/1‑94 (MQ=60)
                       GTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGT                                                                        <  SRR4302540.7581597/94‑1 (MQ=60)
                       GTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGT                                                                        >  SRR4302540.6928804/1‑94 (MQ=60)
                       GTATTGTTGGCCTATATAATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGT                                                                        <  SRR4302540.7581598/94‑1 (MQ=60)
                        TATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTT                                                                       <  SRR4302540.9207145/94‑1 (MQ=60)
                        TATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTT                                                                       >  SRR4302540.7429655/1‑94 (MQ=60)
                        TATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTT                                                                       >  SRR4302540.7230303/1‑94 (MQ=60)
                        TATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTT                                                                       >  SRR4302540.9629523/1‑94 (MQ=60)
                         ATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTC                                                                      <  SRR4302540.4968129/94‑1 (MQ=60)
                         ATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTC                                                                      <  SRR4302540.9684518/94‑1 (MQ=60)
                         ATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTC                                                                      >  SRR4302540.8156457/1‑94 (MQ=60)
                         ATTGGTGGCCTATATCATCGCCGGGCTGTGCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTC                                                                      <  SRR4302540.4968128/94‑1 (MQ=60)
                           TGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGC                                                                    >  SRR4302540.10160555/1‑94 (MQ=60)
                           TGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGC                                                                    >  SRR4302540.4129832/1‑94 (MQ=60)
                             TTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCG                                                                  <  SRR4302540.9335538/94‑1 (MQ=60)
                             TTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCG                                                                  <  SRR4302540.1727254/94‑1 (MQ=60)
                              TGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGT                                                                 >  SRR4302540.5012304/1‑94 (MQ=60)
                               GGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTT                                                                >  SRR4302540.689063/1‑94 (MQ=60)
                               GGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTT                                                                >  SRR4302540.3663709/1‑94 (MQ=60)
                               GGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTT                                                                >  SRR4302540.8853559/1‑94 (MQ=60)
                               GGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTT                                                                >  SRR4302540.842925/1‑94 (MQ=60)
                                GCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTT                                                               >  SRR4302540.2272157/1‑94 (MQ=60)
                                GCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTT                                                               >  SRR4302540.2002395/1‑94 (MQ=60)
                                  CTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTAT                                                             >  SRR4302540.2728957/1‑94 (MQ=60)
                                  CTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTAT                                                             >  SRR4302540.8001987/1‑94 (MQ=60)
                                  CTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTAT                                                             <  SRR4302540.2929355/94‑1 (MQ=60)
                                   TATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTGCCGGTTCGGTCGCCGTTTATG                                                            <  SRR4302540.7159569/94‑1 (MQ=60)
                                   TATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATG                                                            <  SRR4302540.10042123/94‑1 (MQ=60)
                                   TATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATG                                                            <  SRR4302540.10042124/94‑1 (MQ=60)
                                    ATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGC                                                           >  SRR4302540.9204970/1‑94 (MQ=60)
                                    ATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGC                                                           >  SRR4302540.4830557/1‑94 (MQ=60)
                                     TATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCG                                                          <  SRR4302540.9531894/94‑1 (MQ=60)
                                      ATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGC                                                         >  SRR4302540.4048450/1‑94 (MQ=60)
                                        CATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCAT                                                       <  SRR4302540.8732163/94‑1 (MQ=60)
                                         CTCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGTGCATC                                                      <  SRR4302540.963733/94‑1 (MQ=60)
                                         CTCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATC                                                      <  SRR4302540.8673557/94‑1 (MQ=60)
                                         ATCGCCGGTCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATC                                                      <  SRR4302540.3778731/94‑1 (MQ=60)
                                         ATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATC                                                      >  SRR4302540.4231266/1‑94 (MQ=60)
                                         ATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGGTACCGGTTCGTTCGCCGTTTATGCGCATC                                                      >  SRR4302540.3769007/1‑94 (MQ=60)
                                          TCGCCGGGGTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCG                                                     <  SRR4302540.6311059/94‑1 (MQ=60)
                                          TCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGAGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCG                                                     <  SRR4302540.9773160/94‑1 (MQ=60)
                                            GCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTT                                                   >  SRR4302540.326399/1‑94 (MQ=60)
                                             CCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTA                                                  <  SRR4302540.2810180/94‑1 (MQ=60)
                                              CGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTAT                                                 >  SRR4302540.7540807/1‑94 (MQ=60)
                                              CGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTAT                                                 >  SRR4302540.7847653/1‑94 (MQ=60)
                                              CGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTAT                                                 >  SRR4302540.7847654/1‑94 (MQ=60)
                                              CGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGCATTGTTTGCCGTTTCTGCGCATCGTTAT                                                 >  SRR4302540.8212691/1‑94 (MQ=60)
                                               GGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTtgcggcttggtttat                                                >  SRR4302540.4256837/1‑79 (MQ=60)
                                               GGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATA                                                >  SRR4302540.5457026/1‑94 (MQ=60)
                                               GGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATA                                                >  SRR4302540.5457027/1‑94 (MQ=60)
                                                GGCTTTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATAT                                               <  SRR4302540.6518608/94‑1 (MQ=60)
                                                GGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATAT                                               <  SRR4302540.6518607/94‑1 (MQ=60)
                                                GGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATAT                                               <  SRR4302540.4028804/94‑1 (MQ=60)
                                                GGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATAT                                               <  SRR4302540.819278/94‑1 (MQ=60)
                                                 GCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATG                                              >  SRR4302540.4935123/1‑94 (MQ=60)
                                                 GCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATG                                              >  SRR4302540.7178468/1‑94 (MQ=60)
                                                 GCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATG                                              <  SRR4302540.5506727/94‑1 (MQ=60)
                                                  CTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGGACTG‑TTGTTCCTCGAACGGGTTGCGGGTTGGTTCGCGGTTTTTGCGCATCGTgttgtgc                                             >  SRR4302540.1050966/1‑87 (MQ=40)
                                                  CTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGA                                             <  SRR4302540.5758354/94‑1 (MQ=60)
                                                  CTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGA                                             <  SRR4302540.4240250/94‑1 (MQ=60)
                                                   TGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAG                                            >  SRR4302540.4341133/1‑94 (MQ=60)
                                                    TTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGC                                           <  SRR4302540.993900/94‑1 (MQ=60)
                                                    GTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGC                                           >  SRR4302540.4526428/1‑94 (MQ=60)
                                                     gtggtctgtgTCATCATGCGTTCAATGGGCGAAATG‑TTGTGCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCC                                          <  SRR4302540.2748925/84‑1 (MQ=60)
                                                     TTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCC                                          <  SRR4302540.3728835/94‑1 (MQ=60)
                                                     TTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCC                                          >  SRR4302540.4218062/1‑94 (MQ=60)
                                                     TTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCC                                          >  SRR4302540.268977/1‑94 (MQ=60)
                                                      TCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCC                                         <  SRR4302540.7516751/94‑1 (MQ=60)
                                                      TCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCC                                         <  SRR4302540.8323767/94‑1 (MQ=60)
                                                      TCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCC                                         <  SRR4302540.668926/94‑1 (MQ=60)
                                                      TCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCC                                         <  SRR4302540.2502137/94‑1 (MQ=60)
                                                       CGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCG                                        >  SRR4302540.921131/1‑94 (MQ=60)
                                                        GTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCGGTTTATGCGCATCGTTATATGAGCCCGT                                       >  SRR4302540.6543335/1‑94 (MQ=60)
                                                         TCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTT                                      >  SRR4302540.8659779/1‑94 (MQ=60)
                                                          CTTTTTCATCTTGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGGTC                                     >  SRR4302540.924865/1‑94 (MQ=60)
                                                          CTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTC                                     >  SRR4302540.5411136/1‑94 (MQ=60)
                                                          CTTTTTCATCATGAGTTCATTGGACGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGaac                                     <  SRR4302540.9038588/94‑4 (MQ=60)
                                                           TTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCT                                    <  SRR4302540.6265255/94‑1 (MQ=60)
                                                           TTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCT                                    <  SRR4302540.713577/94‑1 (MQ=60)
                                                           TTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCT                                    <  SRR4302540.4681980/94‑1 (MQ=60)
                                                           TTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCT                                    <  SRR4302540.1682095/94‑1 (MQ=60)
                                                             TTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTT                                  <  SRR4302540.5286359/94‑1 (MQ=60)
                                                             TTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTT                                  >  SRR4302540.9662421/1‑94 (MQ=60)
                                                             TTTCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTT                                  <  SRR4302540.3224596/94‑1 (MQ=60)
                                                               TCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGG                                >  SRR4302540.6651594/1‑94 (MQ=60)
                                                               TCATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGG                                <  SRR4302540.1111982/94‑1 (MQ=60)
                                                                CATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCGGTTTAGGCGCATCGTTATATGAGCCCGTTCTTTGGT                               >  SRR4302540.7474803/1‑94 (MQ=60)
                                                                CATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGC                               <  SRR4302540.8670621/94‑1 (MQ=60)
                                                                CATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGC                               >  SRR4302540.385334/1‑94 (MQ=60)
                                                                CATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGCTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGC                               >  SRR4302540.1369293/1‑94 (MQ=60)
                                                                 ATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCT                              >  SRR4302540.530359/1‑94 (MQ=60)
                                                                 ATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCT                              >  SRR4302540.7777517/1‑94 (MQ=60)
                                                                 ATCATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTGTATGCGCATCGTTATTTGAGCCCGTTCTTTGGCT                              >  SRR4302540.7777518/1‑94 (MQ=60)
                                                                   CATGCGTTCAATGGGCGAAATG‑TTGTTGCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTAT                            <  SRR4302540.6684914/94‑1 (MQ=60)
                                                                    ATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATC                           >  SRR4302540.6191807/1‑94 (MQ=60)
                                                                    ATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATC                           >  SRR4302540.9938994/1‑94 (MQ=60)
                                                                    ATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATC                           <  SRR4302540.5192398/94‑1 (MQ=60)
                                                                    ATGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATC                           >  SRR4302540.8704218/1‑94 (MQ=60)
                                                                     TGCGTTTAATGGGCGAAATGTTTGTCCC‑CGAACCGGTTACCGGGTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCT                          <  SRR4302540.6134674/94‑1 (MQ=60)
                                                                     TGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCT                          <  SRR4302540.4757852/94‑1 (MQ=60)
                                                                     TGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCT                          <  SRR4302540.2742898/94‑1 (MQ=60)
                                                                     TGCGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCT                          <  SRR4302540.4937476/94‑1 (MQ=60)
                                                                      gctgTCAATGTGCGATATG‑TTGTGCTTCGAACCTGTTCCCGGTTCGTTCGCCGTTTATGCGCAGCGTTATATGAGCCCGTTCTTTGGCTATCTC                         <  SRR4302540.3629785/90‑1 (MQ=60)
                                                                       CGTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCA                        >  SRR4302540.4504177/1‑94 (MQ=60)
                                                                        GTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCGTCGTTATATGAGCCCGTTCTTTGGCTATCTCAC                       <  SRR4302540.701968/94‑1 (MQ=60)
                                                                        GTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCAC                       <  SRR4302540.4693262/94‑1 (MQ=60)
                                                                        GTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCAC                       >  SRR4302540.7771697/1‑94 (MQ=60)
                                                                        GTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCAC                       <  SRR4302540.7678007/94‑1 (MQ=60)
                                                                        GTTCAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCAC                       <  SRR4302540.7517901/94‑1 (MQ=60)
                                                                         TTCAATGGGCGAGATG‑TTGTGCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACC                      <  SRR4302540.3092051/94‑1 (MQ=60)
                                                                         TTCAAGGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACC                      <  SRR4302540.1897860/94‑1 (MQ=60)
                                                                           CAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGC                    >  SRR4302540.4562162/1‑94 (MQ=60)
                                                                           CAATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGC                    >  SRR4302540.10041242/1‑94 (MQ=60)
                                                                             ATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCT                  <  SRR4302540.5845658/94‑1 (MQ=60)
                                                                             ATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCT                  >  SRR4302540.6624815/1‑94 (MQ=60)
                                                                             ATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCT                  <  SRR4302540.2790305/94‑1 (MQ=60)
                                                                             ATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCT                  <  SRR4302540.4519913/94‑1 (MQ=60)
                                                                             ATGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCT                  >  SRR4302540.7170418/1‑94 (MQ=60)
                                                                             ATGGGCGAAATG‑TTGTTCCTCGAACCGGGTACCGGTTCGGTCGCCGCTTATGCGCCTCGTTATATCAGCCCGTTCTTTGGCTATCTCACCGCCT                  >  SRR4302540.6115938/1‑94 (MQ=60)
                                                                              TGGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTG                 >  SRR4302540.7331892/1‑94 (MQ=60)
                                                                               GGGCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGG                <  SRR4302540.1836001/94‑1 (MQ=60)
                                                                                 GCGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTC              >  SRR4302540.7917053/1‑94 (MQ=60)
                                                                                  CGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCT             >  SRR4302540.5115718/1‑94 (MQ=60)
                                                                                  CGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCT             >  SRR4302540.8113513/1‑94 (MQ=60)
                                                                                  CGAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCT             >  SRR4302540.4335551/1‑94 (MQ=60)
                                                                                   GAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTT            >  SRR4302540.9531303/1‑94 (MQ=60)
                                                                                   GAAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCACGTTCTTTGGCTATCTCACCGCCTGGTCTT            >  SRR4302540.3453517/1‑94 (MQ=60)
                                                                                    AAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTA           >  SRR4302540.5475707/1‑94 (MQ=60)
                                                                                    AAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTA           >  SRR4302540.2222610/1‑94 (MQ=60)
                                                                                    AAATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGGTCTTTGGCTATCTCACCGCCTGGTCTTA           >  SRR4302540.3647815/1‑94 (MQ=60)
                                                                                     AATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTAC          <  SRR4302540.9905461/94‑1 (MQ=60)
                                                                                     AATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTAC          >  SRR4302540.1429715/1‑94 (MQ=60)
                                                                                      ATG‑TTGTTCCTCGCACAGGTTACCGGTTCGTTCGCCCTGTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACT         <  SRR4302540.9248408/94‑1 (MQ=60)
                                                                                      ATG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACT         >  SRR4302540.10051638/1‑94 (MQ=60)
                                                                                       TG‑TTGTTCCTCGAACCGGTTGCCGATTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTG        <  SRR4302540.2444197/94‑1 (MQ=60)
                                                                                       TG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTG        <  SRR4302540.3293890/94‑1 (MQ=60)
                                                                                       TG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTG        <  SRR4302540.8849122/94‑1 (MQ=60)
                                                                                       TG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTG        <  SRR4302540.6335060/94‑1 (MQ=60)
                                                                                       TG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTG        >  SRR4302540.1248832/1‑94 (MQ=60)
                                                                                       TG‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTG        <  SRR4302540.4372597/94‑1 (MQ=60)
                                                                                        G‑TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGG       >  SRR4302540.6439309/1‑94 (MQ=60)
                                                                                          TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGT      <  SRR4302540.9270618/94‑1 (MQ=60)
                                                                                          TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGT      >  SRR4302540.5292065/1‑94 (MQ=60)
                                                                                          TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGT      <  SRR4302540.908903/94‑1 (MQ=60)
                                                                                          TTGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGT      <  SRR4302540.7899402/94‑1 (MQ=60)
                                                                                          TTGTGCCTCGAACCGGTTACCGGTGCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGT      <  SRR4302540.1680912/94‑1 (MQ=60)
                                                                                           TGTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTT     >  SRR4302540.1348211/1‑94 (MQ=60)
                                                                                            GTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTT    <  SRR4302540.4684204/94‑1 (MQ=60)
                                                                                            GTTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTT    <  SRR4302540.1267699/94‑1 (MQ=60)
                                                                                             TTCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTA   >  SRR4302540.3343782/1‑94 (MQ=60)
                                                                                              TCCTCGAAGCGGTTACCGTTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTAT  <  SRR4302540.9107628/94‑1 (MQ=60)
                                                                                              TCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTAT  <  SRR4302540.3681753/94‑1 (MQ=60)
                                                                                              TCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTAT  <  SRR4302540.4282542/94‑1 (MQ=60)
                                                                                              TCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTAT  <  SRR4302540.5337553/94‑1 (MQ=60)
                                                                                              TCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTAT  >  SRR4302540.4681604/1‑94 (MQ=60)
                                                                                              |                                                                                             
CCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAATG‑TTGTCCCTCGAACCGGTTACCGGTTCGTTCGCCGTTTATGCGCATCGTTATATGAGCCCGTTCTTTGGCTATCTCACCGCCTGGTCTTACTGGTTTAT  >  NC_000913/3978609‑3978795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑