Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	1548	369.5	20095359	96.9%	19472402	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,980,297	(C)_8→9	noncoding (79/87 nt)	leuT →	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,980,289	1	.	C	83.5%	721.6 / 151.3	284	noncoding (71/87 nt)	leuT	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base . (19/28); new base C (115/122); total (134/150)
Fisher's exact test for biased strand distribution p-value = 3.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 358 total aligned reads displayed.

CATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTT  >  NC_000913/3980197‑3980381

cATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑t                                                                                                 >  1:9855882/1‑93 (MQ=255)

cATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑t                                                                                                 >  2:1470875/1‑93 (MQ=255)

cATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑t                                                                                                 >  2:6367754/1‑93 (MQ=255)

  ttattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc                                                                                             >  1:6429906/1‑93 (MQ=255)

  ttattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc                                                                                             >  1:7571904/1‑93 (MQ=255)

  ttattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc                                                                                             >  2:7072915/1‑93 (MQ=255)

   tattaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc‑c                                                                                           >  2:1010271/1‑93 (MQ=255)

     ttaGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAAG‑‑T‑‑cc‑ccc                                                                                         >  2:9574456/1‑93 (MQ=255)

      taGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc‑cccc                                                                                        <  1:764432/93‑1 (MQ=255)

        gAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGGCGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc‑cccccc                                                                                      <  2:1715815/93‑1 (MQ=255)

        gAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc‑ccccgc                                                                                      <  2:2988272/93‑3 (MQ=255)

        gAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc‑cccccc                                                                                      <  2:4080050/93‑1 (MQ=255)

        gAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc‑cccccc                                                                                      >  1:1283228/1‑93 (MQ=255)

        gAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc‑cccccc                                                                                      >  1:1204090/1‑93 (MQ=255)

          aGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc‑ccccccct                                                                                    <  1:2268264/93‑3 (MQ=255)

          aGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc‑ccccccct                                                                                    <  2:7502/93‑3 (MQ=255)

          aGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGCGGGGTCAAG‑‑T‑‑gc‑cccccccc                                                                                    >  2:3671242/1‑91 (MQ=14)

           gTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc‑ccccccctc                                                                                   >  2:9529981/1‑90 (MQ=255)

            ttGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc‑ccccccctcg                                                                                  >  1:5097185/1‑89 (MQ=255)

            ttGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑cc‑ccccccctcg                                                                                  >  2:8637209/1‑89 (MQ=255)

                  cAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGcaccac                                                                             <  1:1095522/93‑1 (MQ=16)

                   aaTGCGAAGGTGGCTGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑CC‑CCCCCCTCGCCCCACGa                                                                           <  1:9090834/93‑1 (MQ=255)

                    aggCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGa                                                                           <  1:7774696/91‑1 (MQ=255)

                    aTGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGa                                                                           >  1:1462828/1‑93 (MQ=255)

                      gCGAAGGTGGCGGAATTGGAAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACt                                                                         <  2:8960542/93‑1 (MQ=255)

                       cGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACtt                                                                        <  1:3818634/93‑1 (MQ=255)

                        gAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACttt                                                                       <  2:4652069/93‑1 (MQ=255)

                             tGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGC‑C‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGa                                                                  <  1:8155174/93‑1 (MQ=255)

                             tGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGa                                                                  <  2:9328797/93‑1 (MQ=255)

                               gCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCCCC‑CCCCCCTCGCACCACGACTTTAAAGaa                                                                 <  2:5305879/93‑1 (MQ=14)

                               gCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAAAAt                                                                >  1:4202800/1‑93 (MQ=255)

                                cGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAAtt                                                               <  2:5615636/93‑1 (MQ=255)

                                  gAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGa                                                             >  1:8296014/1‑93 (MQ=255)

                                  gAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGa                                                             >  1:4443447/1‑93 (MQ=255)

                                  gAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGaa                                                            >  2:2784278/1‑93 (MQ=255)

                                    aTTGGTAGACGCGCTAGCTTCAGGTTTTAGTGCCCTTACGGACGTGGGGGTTCAAGC‑C‑‑CC‑CCCCCCTCGCACCCCGACTTTAAAGAATTGAAc                                                           <  2:4677293/93‑1 (MQ=17)

                                    aTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAAc                                                           <  2:347433/93‑1 (MQ=255)

                                    aTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACt                                                          >  1:2890085/1‑93 (MQ=255)

                                     ttGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGTGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACt                                                          >  2:2415002/1‑93 (MQ=255)

                                      tGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TCCCC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACt                                                          <  1:6687468/93‑1 (MQ=17)

                                       ggTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGCTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTaa                                                        >  1:5272634/1‑93 (MQ=255)

                                          aGACGCGCTCGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTaaaaa                                                     <  1:5688778/93‑1 (MQ=255)

                                          aGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTaaaaa                                                     >  1:7390251/1‑93 (MQ=255)

                                          aGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTaaaaa                                                     <  1:9424218/93‑1 (MQ=255)

                                           gACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAAt                                                    >  1:6982472/1‑93 (MQ=255)

                                            aCGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAAtt                                                   >  1:9303469/1‑93 (MQ=255)

                                            aCGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCCCGCACCACGACTTTTAAGAATTGAACTAAAAAtt                                                   >  1:5980101/1‑93 (MQ=18)

                                               cgcTAGCTTCAGGTGTTAGTGTCCTTACGGACGTCGGGGGTCAAG‑‑TC‑CC‑CCCCCCTCGCCCCACGACCTTAAAGAATTTAACTAAAAATTTaa                                                >  1:4885400/1‑93 (MQ=18)

                                                 cTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAGC‑C‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaa                                              <  2:8266652/93‑1 (MQ=255)

                                                 cTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑CCACCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaa                                              >  1:3150402/1‑93 (MQ=255)

                                                 cTAGCTTCAGGGGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑CCACCCCCCTCGCAACACGACCTTAAAGAAATGAACTAAAAATTCaaaa                                              >  1:5765502/1‑93 (MQ=18)

                                                  tAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaaa                                             <  2:9831619/93‑1 (MQ=255)

                                                  tAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaaa                                             <  2:7319810/93‑1 (MQ=255)

                                                  tAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCaaaaa                                             >  2:5147530/1‑93 (MQ=255)

                                                   aGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAg                                            <  1:3080410/93‑1 (MQ=255)

                                                    gCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGc                                           <  1:9608465/93‑1 (MQ=255)

                                                      ttCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAg                                         >  2:658858/1‑93 (MQ=255)

                                                       tCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGt                                        <  1:6791214/93‑1 (MQ=255)

                                                        cAGGTGTTAGTGTCCTTACGGACGTGTGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTa                                       <  2:9133312/93‑1 (MQ=255)

                                                        cAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTa                                       <  2:5845432/93‑1 (MQ=255)

                                                        cAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTa                                       <  1:8727229/93‑1 (MQ=255)

                                                        cAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTa                                       <  2:1897926/93‑1 (MQ=255)

                                                         aGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAt                                      <  2:1576138/93‑1 (MQ=255)

                                                          ggTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAtt                                     <  2:2672384/93‑1 (MQ=255)

                                                          ggTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAtt                                     >  1:3385240/1‑93 (MQ=255)

                                                          ggTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAtt                                     >  2:4568071/1‑93 (MQ=255)

                                                          ggTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑G‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAttt                                    <  2:7968334/93‑1 (MQ=255)

                                                           gtgtTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAttt                                    <  1:3936342/93‑1 (MQ=255)

                                                           gtgtTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAttt                                    <  1:2037292/93‑1 (MQ=255)

                                                           gtgtTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTc                                   >  1:5403870/1‑93 (MQ=255)

                                                            tgtTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTc                                   <  2:1220292/93‑1 (MQ=255)

                                                             gtTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCg                                  <  2:3884534/93‑1 (MQ=255)

                                                               tAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGc                                <  1:2686068/93‑1 (MQ=255)

                                                               tAGTGTCCTTACGGACGTGGGGGGTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGc                                <  1:3334615/93‑1 (MQ=255)

                                                                aGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGa                              <  2:5426187/93‑1 (MQ=255)

                                                                aGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGa                              <  1:7975298/93‑1 (MQ=255)

                                                                 gtgtCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGa                              <  2:4352917/93‑1 (MQ=255)

                                                                 gtgtCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGa                              <  1:3059367/93‑1 (MQ=255)

                                                                 gtgtCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAg                             <  1:6927142/93‑1 (MQ=255)

                                                                    tCCTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTa                           <  2:1769570/93‑1 (MQ=255)

                                                                    gccTTACGGACGTGGGGGTTCAAG‑‑T‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAg                          <  1:2832333/92‑1 (MQ=255)

                                                                     ccTTACGGACGTGGGGGTTCAAG‑‑TCCCC‑CCCCCCTCCCACCCCGACTTTTAAAAATTTAAATAAAAAATTAAAAAACAGTATTTTGGCGAgga                           >  1:8968266/1‑91 (MQ=12)

                                                                     ccTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAg                          <  1:1342550/93‑1 (MQ=255)

                                                                     ccTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAg                          <  1:9910923/93‑1 (MQ=255)

                                                                      cTTACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgc                         <  1:8802786/93‑1 (MQ=255)

                                                                       ttACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgcg                        <  1:864918/93‑1 (MQ=255)

                                                                        tACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgcgc                       >  1:10022965/1‑93 (MQ=255)

                                                                        tACGGACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAgcga                       >  1:2462480/1‑92 (MQ=255)

                                                                          cGGACGTGGGGGTTCAAG‑‑TCCCC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCa                      <  2:3188170/93‑1 (MQ=39)

                                                                            gACGTGGGGGTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCCGCt                   <  2:8453762/93‑1 (MQ=255)

                                                                             gcGTGGGGGTTCAAGCCC‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCt                   <  2:6123861/92‑1 (MQ=255)

                                                                                 gggggTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTa              <  1:1840608/93‑1 (MQ=255)

                                                                                 gggggTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTa              <  1:6546340/93‑1 (MQ=255)

                                                                                 gggggTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTa              <  1:169463/93‑1 (MQ=255)

                                                                                  ggggTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAg             >  1:4760695/1‑93 (MQ=255)

                                                                                  ggggTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAg             <  2:6946753/93‑1 (MQ=255)

                                                                                    ggTTCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAgcg           >  2:6761316/1‑93 (MQ=255)

                                                                                    ggTTCAAG‑‑T‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAgcgc          >  1:6112544/1‑93 (MQ=255)

                                                                                      ttCAAG‑‑TC‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGCAGCGCAGCTTGGTAGCGCa         >  1:6219563/1‑93 (MQ=255)

                                                                                           g‑‑T‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGt   >  2:8177696/1‑93 (MQ=255)

CATTATTAGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAAG‑‑T‑‑CC‑CCCCCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTT  >  NC_000913/3980197‑3980381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 226 total aligned reads displayed.

AGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑T‑‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAG  >  NC_000913/3980204‑3980390

AGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGCCGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑T‑‑CC‑C‑CC‑CCC                                                                                               <  SRR4302540.127019/94‑1 (MQ=33)

AGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑T‑‑CC‑C‑CC‑CCC                                                                                               >  SRR4302540.3167437/1‑94 (MQ=34)

AGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑T‑‑CC‑C‑CC‑CCC                                                                                               >  SRR4302540.1296457/1‑94 (MQ=34)

AGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑T‑‑CC‑C‑CC‑CCC                                                                                               >  SRR4302540.1216459/1‑94 (MQ=34)

   AGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑T‑‑CC‑C‑CC‑CCCctc                                                                                            <  SRR4302540.2292599/94‑4 (MQ=27)

   AGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑T‑‑CC‑C‑CC‑CCCctc                                                                                            >  SRR4302540.10105328/1‑91 (MQ=27)

    GTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCG                                                                                            >  SRR4302540.5152343/1‑94 (MQ=23)

    GTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑T‑‑CC‑C‑CC‑CCCTCGC                                                                                           <  SRR4302540.7389149/94‑1 (MQ=24)

     TTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGC                                                                                           >  SRR4302540.6949028/1‑94 (MQ=20)

       GTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTTAA‑‑G‑T‑‑GC‑C‑CT‑CCCTCTCACC                                                                                        >  SRR4302540.5521293/1‑94 (MQ=8)

       GTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTGAA‑‑G‑T‑‑CC‑C‑CC‑CCCccccccc                                                                                        >  SRR4302540.3798132/1‑87 (MQ=10)

         GACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCA                                                                                       <  SRR4302540.1673909/94‑1 (MQ=5)

           CAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACG                                                                                     <  SRR4302540.1106785/94‑1 (MQ=5)

           CAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACG                                                                                     <  SRR4302540.1321605/94‑1 (MQ=5)

           CAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACG                                                                                     >  SRR4302540.8668202/1‑94 (MQ=5)

            AATGCGAAGGTGGCTGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑T‑‑CC‑C‑CC‑CCCTCGCCCCACGAC                                                                                   <  SRR4302540.9200051/94‑1 (MQ=5)

            AATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TCCCC‑C‑CC‑CCCTCCCACCACG                                                                                     >  SRR4302540.6412910/1‑94 (MQ=0)

            AATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑T‑‑CC‑C‑CC‑CCCctcgcccccaga                                                                                   >  SRR4302540.3069724/1‑82 (MQ=0)

            AATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGA                                                                                    >  SRR4302540.1478773/1‑94 (MQ=6)

             ATGCGAGGGTGGCGGATTTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTAACGGCCGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGAC                                                                                   <  SRR4302540.9066210/94‑1 (MQ=3)

             ATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGAC                                                                                   >  SRR4302540.779688/1‑94 (MQ=5)

             AGGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGAC                                                                                   <  SRR4302540.7865582/94‑1 (MQ=5)

              TGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TG‑CC‑C‑CC‑CCCCCGCACCACcaat                                                                                  >  SRR4302540.4236040/1‑90 (MQ=0)

              TGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCCCGACT                                                                                  >  SRR4302540.4920458/1‑94 (MQ=0)

              TGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACT                                                                                  <  SRR4302540.8014285/94‑1 (MQ=7)

              AGCGAAGGTTGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACT                                                                                  <  SRR4302540.3386346/94‑1 (MQ=7)

               GCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTT                                                                                 <  SRR4302540.7023638/94‑1 (MQ=10)

                TGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAA‑‑G‑T‑‑CC‑C‑CC‑CCCCCGCACCACGCCTTTA                                                                               >  SRR4302540.5529134/1‑94 (MQ=2)

                CGAAGGTGGCGGAATTTGTAGACGCGCTATCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTT                                                                                <  SRR4302540.2084032/94‑1 (MQ=11)

                CGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTT                                                                                <  SRR4302540.9506081/94‑1 (MQ=12)

                CGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTT                                                                                <  SRR4302540.3860202/94‑1 (MQ=12)

                CGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAA‑‑G‑T‑‑CC‑C‑CC‑CCCctcgcccccccccctt                                                                               >  SRR4302540.7649126/1‑78 (MQ=0)

                 GAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCCCGACTTTA                                                                               >  SRR4302540.2003560/1‑94 (MQ=4)

                 GAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTA                                                                               >  SRR4302540.5537880/1‑94 (MQ=20)

                 GAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTA                                                                               <  SRR4302540.579749/94‑1 (MQ=20)

                 GAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑T‑‑CC‑C‑CC‑CCCctcccacccccccgttt                                                                              >  SRR4302540.3906996/1‑77 (MQ=0)

                 GAAGGCGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTA                                                                               <  SRR4302540.3408223/94‑1 (MQ=19)

                  AAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAA                                                                              >  SRR4302540.1675093/1‑94 (MQ=22)

                  AAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAA                                                                              >  SRR4302540.8342598/1‑94 (MQ=22)

                   AGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAA                                                                             <  SRR4302540.6122586/94‑1 (MQ=25)

                   AGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCCCGCACCACGACCTTAAA                                                                             >  SRR4302540.2257785/1‑94 (MQ=0)

                   AGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑T‑‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAG                                                                            <  SRR4302540.3884803/94‑1 (MQ=29)

                    GGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAG                                                                            <  SRR4302540.8323117/94‑1 (MQ=27)

                    GGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAG                                                                            >  SRR4302540.3461858/1‑94 (MQ=27)

                    GGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAG                                                                            <  SRR4302540.8368492/94‑1 (MQ=27)

                    GGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAG                                                                            >  SRR4302540.1296805/1‑94 (MQ=27)

                    GGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAG                                                                            >  SRR4302540.1296806/1‑94 (MQ=27)

                    GGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAA                                                                            >  SRR4302540.7548979/1‑94 (MQ=24)

                     GTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TCCCC‑C‑CC‑CCCCCGCACCACGACTTTAAAG                                                                            >  SRR4302540.6447569/1‑94 (MQ=9)

                      TGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑GCC‑‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAA                                                                          <  SRR4302540.8249861/94‑1 (MQ=30)

                      GGGCGGAATTGGTAGTCCCGCTAGCTTCAGGTGTTCGTGTCCTTACGGACGTGGGGTTTCAACCC‑C‑‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGA                                                                           <  SRR4302540.5352007/94‑1 (MQ=22)

                      GGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAA                                                                          <  SRR4302540.4866654/94‑1 (MQ=32)

                       GGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAAAAT                                                                         >  SRR4302540.4248537/1‑94 (MQ=24)

                       GGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAA‑‑G‑T‑‑CC‑C‑CC‑CCCctccccccaccactttaaaaaat                                                                        >  SRR4302540.7207813/1‑71 (MQ=0)

                        GCGGAATTGGTAGACGCGCTGGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑GCC‑‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATT                                                                        <  SRR4302540.3042121/94‑1 (MQ=34)

                          GGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGA                                                                      >  SRR4302540.4491604/1‑94 (MQ=42)

                          GGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGA                                                                      >  SRR4302540.10058600/1‑94 (MQ=42)

                          GGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGA                                                                      >  SRR4302540.8392343/1‑94 (MQ=42)

                          GGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGA                                                                      <  SRR4302540.7676443/94‑1 (MQ=42)

                          GGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTTAA‑‑G‑T‑‑CC‑C‑CC‑CCCcccccccccccacctttaaaaaatta                                                                     >  SRR4302540.7438443/1‑68 (MQ=0)

                          GGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGA                                                                      >  SRR4302540.4342627/1‑94 (MQ=40)

                          GGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGGTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGA                                                                      >  SRR4302540.9110060/1‑94 (MQ=40)

                           GAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAA                                                                     >  SRR4302540.1247318/1‑94 (MQ=44)

                           GAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAA                                                                     <  SRR4302540.6624379/94‑1 (MQ=44)

                           GAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAA                                                                     <  SRR4302540.822713/94‑1 (MQ=44)

                            AATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAAC                                                                    <  SRR4302540.2648045/94‑1 (MQ=47)

                             ATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑T‑‑CC‑CGCC‑CCCTTGCACCCCGCCTTTACAGAATTGAACA                                                                   >  SRR4302540.1415140/1‑94 (MQ=5)

                             ATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGGGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACT                                                                   <  SRR4302540.11346/94‑1 (MQ=47)

                               TGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TCCCC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTA                                                                  <  SRR4302540.6761307/94‑1 (MQ=51)

                               TGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAA                                                                 >  SRR4302540.272134/1‑94 (MQ=54)

                               TGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGCTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAA                                                                 >  SRR4302540.5330186/1‑94 (MQ=52)

                                GGTAGACGCGCTAGGTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TCCCC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAA                                                                 <  SRR4302540.9415887/94‑1 (MQ=51)

                                 GTGGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACTTGGGGGTTCAA‑‑G‑T‑‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAA                                                              <  SRR4302540.5426894/94‑1 (MQ=60)

                                 GTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑T‑‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAA                                                              <  SRR4302540.6230377/94‑1 (MQ=60)

                                  TAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAA                                                              >  SRR4302540.7472593/1‑94 (MQ=60)

                                  TAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAA                                                              <  SRR4302540.9083639/94‑1 (MQ=60)

                                  GAGACGCGCTAGCTTCAGGTCTTAGTGTCCTGACGGACGGGGGGGGTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAA                                                              <  SRR4302540.480758/94‑1 (MQ=51)

                                   AGGCGCGCTAGGTTCAGGTGTTAGTGTCCTTACGGAAGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAAT                                                             <  SRR4302540.7835832/94‑1 (MQ=57)

                                   AGACGCGCTCGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAAT                                                             <  SRR4302540.5751396/94‑1 (MQ=60)

                                   AGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAAT                                                             >  SRR4302540.7060024/1‑94 (MQ=60)

                                   AGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAAT                                                             <  SRR4302540.5756704/94‑1 (MQ=60)

                                   AGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAAT                                                             <  SRR4302540.9536208/94‑1 (MQ=60)

                                   AGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑T‑‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATT                                                            >  SRR4302540.3252442/1‑94 (MQ=60)

                                    GACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATT                                                            >  SRR4302540.6326640/1‑94 (MQ=60)

                                    GACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATT                                                            >  SRR4302540.9414593/1‑94 (MQ=60)

                                    GACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCCCGCACCACGACTTTTAAGAATTGAACTAAAAATT                                                            >  SRR4302540.6046307/1‑94 (MQ=40)

                                      CGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TCCCC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTC                                                           <  SRR4302540.9731846/94‑1 (MQ=60)

                                      CGCCCTAGCTTCAGTGGTTAGGGTCCGTGCGGACGTGGTGGGTCAA‑‑GCT‑‑CC‑C‑CC‑CCCTTGCACCACGACTTTAAAGAATTGAACTAAAAATTCA                                                          <  SRR4302540.7856258/94‑1 (MQ=38)

                                       GCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTCGGGGGTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCCCCACGACCTTAAAGAATTTAACTAAAAATTTAA                                                         >  SRR4302540.4937879/1‑94 (MQ=25)

                                        CGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTTAAAAATTGAACTAAAAATTCAAA                                                        >  SRR4302540.2398569/1‑94 (MQ=47)

                                        CGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAA                                                        <  SRR4302540.4205772/94‑1 (MQ=60)

                                         GCTAGCTTCAGGTTTTAGTGTCCTTACGGACTTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAA                                                       <  SRR4302540.2908852/94‑1 (MQ=60)

                                         GCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑T‑‑CCAC‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAA                                                       >  SRR4302540.3184709/1‑94 (MQ=60)

                                         GCTAGCTTCAGGGGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑T‑‑CCAC‑CC‑CCCTCGCAACACGACCTTAAAGAAATGAACTAAAAATTCAAAA                                                       >  SRR4302540.5829162/1‑94 (MQ=41)

                                          CTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAA                                                      <  SRR4302540.5159328/94‑1 (MQ=60)

                                          CTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAA                                                      >  SRR4302540.9930809/1‑94 (MQ=60)

                                          CTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACCACTTTAAAGAATTGCAATAAAAATTCAAAAA                                                      >  SRR4302540.8239188/1‑94 (MQ=39)

                                            AGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGC                                                    <  SRR4302540.3114214/94‑1 (MQ=60)

                                             GCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCA                                                   >  SRR4302540.1050687/1‑94 (MQ=60)

                                             GCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCA                                                   <  SRR4302540.9721771/94‑1 (MQ=60)

                                              CTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAG                                                  <  SRR4302540.7887891/94‑1 (MQ=60)

                                              CTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAG                                                  >  SRR4302540.4469525/1‑94 (MQ=60)

                                               TTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TCCCC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAG                                                  >  SRR4302540.3089692/1‑94 (MQ=60)

                                               TTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCGCGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGT                                                 <  SRR4302540.5180025/94‑1 (MQ=60)

                                                TCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTA                                                <  SRR4302540.6866321/94‑1 (MQ=60)

                                                 CAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAT                                               <  SRR4302540.7031983/94‑1 (MQ=60)

                                                 CAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTAT                                               <  SRR4302540.8828574/94‑1 (MQ=60)

                                                  AGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATT                                              >  SRR4302540.3422187/1‑94 (MQ=60)

                                                  AGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGAATT                                              >  SRR4302540.2884441/1‑94 (MQ=60)

                                                   GGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTT                                             <  SRR4302540.6919613/94‑1 (MQ=60)

                                                   GGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑T‑‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTC                                            >  SRR4302540.5463281/1‑94 (MQ=60)

                                                   GGTGTGAGTGTCATCGCGGACGTGGGTGTTCAA‑‑G‑G‑‑CC‑C‑CCACCCTCGCACCACGACTGTAAAGAATTGAATTAAAAATTCAAAAAGCAGTATTT                                             <  SRR4302540.6919623/94‑1 (MQ=55)

                                                    GTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTC                                            <  SRR4302540.8811385/94‑1 (MQ=60)

                                                    GTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTC                                            >  SRR4302540.1669398/1‑94 (MQ=60)

                                                    GTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTC                                            <  SRR4302540.2059256/94‑1 (MQ=60)

                                                    GTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTC                                            <  SRR4302540.6920873/94‑1 (MQ=60)

                                                    GTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTC                                            <  SRR4302540.3978883/94‑1 (MQ=60)

                                                     TGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCG                                           >  SRR4302540.6203607/1‑94 (MQ=60)

                                                      GTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGG                                          >  SRR4302540.2884907/1‑94 (MQ=60)

                                                        TAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCG                                        <  SRR4302540.2715105/94‑1 (MQ=60)

                                                        TAGTGTCCTTACGGACGTGGGGGGTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCG                                        <  SRR4302540.3371233/94‑1 (MQ=60)

                                                         AGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑T‑‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAG                                      <  SRR4302540.8068213/94‑1 (MQ=60)

                                                          GTGTCCTTACGGACGTGGGGGTTCAG‑‑G‑T‑‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGT                                     <  SRR4302540.8881430/94‑1 (MQ=60)

                                                          GTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGGACTAAAAATTCAAAAAGCAGTATTTCGGCGAG                                      >  SRR4302540.6804542/1‑94 (MQ=60)

                                                          GTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAG                                      >  SRR4302540.6732054/1‑94 (MQ=60)

                                                          GTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAG                                      <  SRR4302540.3092933/94‑1 (MQ=60)

                                                          GTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAAAATTGAACTAAAAATTAAAAAAGCAGTATTTCGGCGAG                                      >  SRR4302540.6962072/1‑94 (MQ=60)

                                                          GTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑T‑‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGT                                     <  SRR4302540.7004233/94‑1 (MQ=60)

                                                           ttgtgttcctacgggcggtggggttccaggccCC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGC                                         <  SRR4302540.816072/62‑1 (MQ=60)

                                                           TGTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGT                                     <  SRR4302540.5239036/94‑1 (MQ=60)

                                                            GTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTA                                    >  SRR4302540.2433345/1‑94 (MQ=60)

                                                            GTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTA                                    <  SRR4302540.36038/94‑1 (MQ=60)

                                                            GTCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGGA                                    >  SRR4302540.2664663/1‑94 (MQ=60)

                                                             TCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAG                                   >  SRR4302540.3148614/1‑94 (MQ=60)

                                                             TCCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAG                                   <  SRR4302540.2839930/94‑1 (MQ=60)

                                                             GCCTTACGGACGTGGGGGTTCAA‑‑G‑T‑‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGC                                  <  SRR4302540.2862966/94‑1 (MQ=60)

                                                              CCTTACGGACGTGGGGGTTCAA‑‑GCC‑‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGC                                  <  SRR4302540.3083473/94‑1 (MQ=60)

                                                              CCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGC                                  >  SRR4302540.9492995/1‑94 (MQ=60)

                                                              CCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGC                                  <  SRR4302540.10026683/94‑1 (MQ=60)

                                                              CCTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCCCCCCCCCCGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGcgaggaagc                                  >  SRR4302540.4612292/1‑85 (MQ=52)

                                                               CTTGCGGCCGTGGGGGTTCAAG‑G‑C‑‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCG                                 <  SRR4302540.8904549/94‑1 (MQ=60)

                                                               CTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCG                                 >  SRR4302540.9824145/1‑94 (MQ=60)

                                                               CTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCG                                 <  SRR4302540.8904548/94‑1 (MQ=60)

                                                               CTTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGAAGCG                                 >  SRR4302540.3313699/1‑94 (MQ=60)

                                                                TTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGC                                <  SRR4302540.874103/94‑1 (MQ=60)

                                                                TTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGC                                >  SRR4302540.10139599/1‑94 (MQ=60)

                                                                TTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGC                                >  SRR4302540.1458091/1‑94 (MQ=60)

                                                                TTACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGA                                >  SRR4302540.2488982/1‑94 (MQ=60)

                                                                 TACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCA                               <  SRR4302540.7747794/94‑1 (MQ=60)

                                                                 TACGGACGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCA                               <  SRR4302540.5020228/94‑1 (MQ=60)

                                                                       CGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGG                         >  SRR4302540.1728043/1‑94 (MQ=60)

                                                                       CGTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGG                         >  SRR4302540.6584572/1‑94 (MQ=60)

                                                                        GTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCCCCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGT                        >  SRR4302540.5927570/1‑94 (MQ=60)

                                                                        GTGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGT                        >  SRR4302540.7666554/1‑94 (MQ=60)

                                                                         TGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTTAAAAATTGAACTAAAAATTCAAAAAACAGTATTTCGGCGAGTAGCGCAGCTTTGTA                       >  SRR4302540.1878483/1‑94 (MQ=60)

                                                                         TGGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTA                       >  SRR4302540.6737450/1‑94 (MQ=60)

                                                                         TGGGGGGTCAA‑‑G‑TCCCC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGT                        <  SRR4302540.3642720/94‑1 (MQ=60)

                                                                          GGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAG                      >  SRR4302540.4811498/1‑94 (MQ=60)

                                                                          GGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAG                      <  SRR4302540.1860773/94‑1 (MQ=60)

                                                                          GGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAG                      <  SRR4302540.171160/94‑1 (MQ=60)

                                                                          GGGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAG                      <  SRR4302540.6618966/94‑1 (MQ=60)

                                                                          GGGGGTTCAA‑‑G‑T‑‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGC                     <  SRR4302540.3095700/94‑1 (MQ=60)

                                                                           GGGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGC                     >  SRR4302540.6194509/1‑94 (MQ=60)

                                                                            GGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCG                    <  SRR4302540.8416316/94‑1 (MQ=60)

                                                                            GGGTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCG                    <  SRR4302540.9216784/94‑1 (MQ=60)

                                                                            GGGTTCAA‑‑G‑T‑‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGC                   >  SRR4302540.6180612/1‑94 (MQ=60)

                                                                              GTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCA                  >  SRR4302540.9337800/1‑94 (MQ=60)

                                                                              GTTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGCAGCGCAGCTTGGTAGCGCA                  >  SRR4302540.6288754/1‑94 (MQ=60)

                                                                               TTCAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAA                 >  SRR4302540.9689995/1‑94 (MQ=60)

                                                                                 CAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACT               >  SRR4302540.3647586/1‑94 (MQ=60)

                                                                                 CAA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACG               >  SRR4302540.5939846/1‑94 (MQ=60)

                                                                                  AA‑‑G‑TCCCC‑C‑CC‑CCCTCGCACCACAACTTTAAAGAATTGAACTAAAAATTCAAAAAGAAGTATTTCGGCGAGTAGCGCAGCTTGGTAGGGCAACG               >  SRR4302540.9612865/1‑94 (MQ=60)

                                                                                  AA‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTG              <  SRR4302540.5220356/94‑1 (MQ=60)

                                                                                   A‑‑G‑TC‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGG             >  SRR4302540.6822744/1‑94 (MQ=60)

                                                                                      G‑C‑‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTT           <  SRR4302540.2906392/94‑1 (MQ=60)

                                                                                        C‑‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTT          <  SRR4302540.9775075/94‑1 (MQ=60)

                                                                                        C‑‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTT          <  SRR4302540.8737543/94‑1 (MQ=60)

                                                                                        C‑‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTT          <  SRR4302540.3714381/94‑1 (MQ=60)

                                                                                        C‑‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTT          <  SRR4302540.880156/94‑1 (MQ=60)

                                                                                         tcCC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTT           >  SRR4302540.2610993/3‑94 (MQ=60)

                                                                                           CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTG         <  SRR4302540.2538968/94‑1 (MQ=60)

                                                                                            C‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGG        >  SRR4302540.3581520/1‑94 (MQ=60)

                                                                                            C‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGG        >  SRR4302540.8618415/1‑94 (MQ=60)

                                                                                              C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGG       >  SRR4302540.5448887/1‑94 (MQ=60)

                                                                                                CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGA      <  SRR4302540.770375/94‑1 (MQ=60)

                                                                                                CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGA      <  SRR4302540.7710274/94‑1 (MQ=60)

                                                                                                 C‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGAC     <  SRR4302540.7479926/94‑1 (MQ=60)

                                                                                                   CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTCGAGCAGCTTGGTAGCGCATCTGGTTTGGGACC    >  SRR4302540.482026/1‑94 (MQ=60)

                                                                                                   CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACC    >  SRR4302540.387710/1‑94 (MQ=60)

                                                                                                   CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACC    >  SRR4302540.1081914/1‑94 (MQ=60)

                                                                                                   CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACC    >  SRR4302540.8982634/1‑94 (MQ=60)

                                                                                                   CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACC    <  SRR4302540.8862421/94‑1 (MQ=60)

                                                                                                   CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACC    >  SRR4302540.482027/1‑94 (MQ=60)

                                                                                                    CCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCA   >  SRR4302540.3811167/1‑94 (MQ=60)

                                                                                                    CCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCA   >  SRR4302540.30156/1‑94 (MQ=60)

                                                                                                    CCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCA   >  SRR4302540.215282/1‑94 (MQ=60)

                                                                                                     CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAG  <  SRR4302540.5501368/94‑1 (MQ=60)

                                                                                                     CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAG  <  SRR4302540.1078558/94‑1 (MQ=60)

                                                                                                     CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAG  <  SRR4302540.5432841/94‑1 (MQ=60)

                                                                                                     CTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAG  >  SRR4302540.3214191/1‑94 (MQ=60)

AGAAGTTGTGACAATGCGAAGGTGGCGGAATTGGTAGACGCGCTAGCTTCAGGTGTTAGTGTCCTTACGGACGTGGGGGTTCAA‑‑G‑T‑‑CC‑C‑CC‑CCCTCGCACCACGACTTTAAAGAATTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAG  >  NC_000913/3980204‑3980390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`